CN111346913A - Plant-microorganism comprehensive remediation system for polluted soil and underground water - Google Patents
Plant-microorganism comprehensive remediation system for polluted soil and underground water Download PDFInfo
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Abstract
本发明提供一种植物‑微生物综合修复污染土壤及地下水系统,主要涉及环境修复技术领域。一种植物‑微生物综合修复污染土壤及地下水系统,包括上游注水井,下游抽水井,聚内交酯限制层,隔膜泵,营养物质注射井,氧气注入井,养物质及微生物填充区,隔水层。本发明的有益效果在于:本发明能够根据土壤与地下水污染分布情况,强制植物根系深入受污染区域的土壤及地下水,同时联合微生物的作用,提高根际微域污染物降解、迁移转化的效率,再结合地下水抽出‑回注方式,不仅增强地下水流通效率,提高土壤及地下水中污染物与植物根系及微生物的接触机率,而且增加地下水曝气,加速有机污染降解。
The invention provides a plant-microorganism comprehensive restoration of polluted soil and groundwater system, and mainly relates to the technical field of environmental restoration. A plant-microorganism integrated remediation system for polluted soil and groundwater, including an upstream injection well, a downstream pumping well, a polylactide restriction layer, a diaphragm pump, a nutrient injection well, an oxygen injection well, a nutrient and microbial filling area, and a water barrier Floor. The beneficial effects of the present invention are: according to the distribution of soil and groundwater pollution, the present invention can force the root system of plants to penetrate deep into the soil and groundwater of the polluted area, and at the same time combine the action of microorganisms to improve the efficiency of degradation, migration and transformation of pollutants in the rhizosphere microdomain, Combined with groundwater extraction-reinjection, it not only enhances groundwater circulation efficiency, increases the contact probability of pollutants in soil and groundwater with plant roots and microorganisms, but also increases groundwater aeration and accelerates the degradation of organic pollution.
Description
技术领域technical field
本发明主要涉及境修复技术,具体是一种植物-微生物综合修复污染土壤及地下水系统。The invention mainly relates to environmental restoration technology, in particular to a plant-microbe comprehensive restoration of polluted soil and groundwater system.
背景技术Background technique
随着我国人口的增长、工业产业化发展进程的加快和人均生活水平的提高,工农业生产和生活污染物大量进入土壤和地下水,对生态安全及人体健康造成极大威胁。2014年发布的《全国土壤污染状况调查公报》以及近年来诸多污染事件的曝光,都表明我国土壤与地下水污染问题日益突出,污染土壤与地下水的修复治理刻不容缓。With the growth of my country's population, the acceleration of industrialization development and the improvement of per capita living standards, a large number of industrial and agricultural production and domestic pollutants enter the soil and groundwater, posing a great threat to ecological security and human health. The "National Soil Pollution Survey Bulletin" released in 2014 and the exposure of many pollution incidents in recent years have shown that the problem of soil and groundwater pollution in my country has become increasingly prominent, and the restoration of contaminated soil and groundwater is urgent.
土壤与地下水的修复方式从根本上可分为物理修复、化学修复、生物修复3大类,而不同方法都有其优缺点,目前,污染物的修复正朝着绿色、环境友好的生物修复以及多元联合杂交的综合修复等技术方向发展。如申请公布号为CN102463253A的发明专利申请公开了一种植物微生物联合修复土壤及浅层地下水有机污染物系统,申请公布号为CN108706820A的发明专利申请公开了一种复合型土壤地下水循环处理装置,申请公布号为CN104801536A的发明专利申请公开了一种利用化学淋洗与微生物相结合修复卤代烃污染场地的方法,申请公布号为CN104692531A的发明专利申请公开了一种地下水重金属污染修复装置。Soil and groundwater remediation methods can be fundamentally divided into three categories: physical remediation, chemical remediation, and bioremediation. Different methods have their own advantages and disadvantages. At present, the remediation of pollutants is moving towards green, environment-friendly bioremediation and The development of technologies such as comprehensive repair of multiple combined hybridization. For example, the invention patent application with the application publication number CN102463253A discloses a plant-microbe joint restoration system for soil and shallow groundwater organic pollutants, and the invention patent application with the application publication number CN108706820A discloses a composite soil and groundwater circulation treatment device. The invention patent application with publication number CN104801536A discloses a method for remediating halogenated hydrocarbon contaminated sites by combining chemical leaching and microorganisms, and the invention patent application with publication number CN104692531A discloses a groundwater heavy metal pollution restoration device.
上述修复系统及方法仅适用于修复浅层地下水或地下水流通缓慢的情况,修复效率不高;采用抽出地表处理后回注的方式,无法改变抽出处理的拖尾效应,且适用的污染物种类较单一,不能很好的解决我国土壤与地下水污染的问题。The above remediation systems and methods are only suitable for remediation of shallow groundwater or slow groundwater circulation, and the remediation efficiency is not high; the method of extracting the surface for treatment and then reinjecting it cannot change the tailing effect of the extraction process, and the applicable pollutant types are relatively small. Single, can not solve the problem of soil and groundwater pollution in my country.
发明内容SUMMARY OF THE INVENTION
为解决现有技术的不足,本发明提供了一种植物-微生物综合修复污染土壤及地下水系统,本发明能够根据土壤与地下水污染分布情况,强制植物根系深入受污染区域的土壤及地下水,同时联合微生物的作用,提高根际微域污染物降解、迁移转化的效率,再结合地下水抽出-回注方式,不仅增强地下水流通效率,提高土壤及地下水中污染物与植物根系及微生物的接触机率,而且增加地下水曝气,加速有机污染降解。In order to solve the deficiencies of the prior art, the present invention provides a plant-microbe comprehensive remediation system for polluted soil and groundwater. The present invention can force the root system of plants to penetrate deep into the soil and groundwater in the polluted area according to the distribution of soil and groundwater pollution. The role of microorganisms can improve the efficiency of degradation, migration and transformation of pollutants in the rhizosphere micro-domain, and combined with groundwater extraction-reinjection, it not only enhances the efficiency of groundwater circulation, but also improves the contact probability of pollutants in soil and groundwater with plant roots and microorganisms. Increase groundwater aeration to accelerate the degradation of organic pollution.
本发明为实现上述目的,通过以下技术方案实现:The present invention is achieved by the following technical solutions in order to achieve the above object:
一种植物-微生物综合修复污染土壤及地下水系统,包括上游注水井,下游抽水井,隔膜泵,隔水层,所述下游抽水井下端设有养物质及微生物填充区,所述养物质及微生物填充区位于地下水位±0.5m,且不穿透隔水层,所述下游抽水井四周设置聚内交酯限制层、营养物质注射井与氧气注入井,所述营养物质注射井上开设注射孔、所述氧气注入井下方开设筛孔。A plant-microbe comprehensive remediation system for polluted soil and groundwater, comprising an upstream water injection well, a downstream pumping well, a diaphragm pump, and a water barrier, and a nutrient and microbial filling area is provided at the lower end of the downstream pumping well, and the nutrient and The filling area is located at the groundwater level ± 0.5m, and does not penetrate the aquifer. The downstream pumping well is surrounded by a polylactide restriction layer, a nutrient injection well and an oxygen injection well. The nutrient injection well is provided with injection holes, Screen holes are provided below the oxygen injection well.
如上所述,所述养物质及微生物填充区内采用放线菌密旋链霉菌Act12与生物炭作为填充物料。As mentioned above, in the nutrient and microorganism filling area, the actinomycetes Streptomyces triceps Act12 and biochar are used as filling materials.
如上所述,所述下游抽水井内种植植物。As mentioned above, plants are grown in the downstream pumping well.
如上所述,所述植物为桦树。As mentioned above, the plant is birch.
如上所述,所述筛孔外周设有3-4层纱网。As mentioned above, there are 3-4 layers of gauze on the periphery of the mesh.
如上所述,在不影响植物正常生长的前提下,在污染区域尽可能密集地种植植物。As mentioned above, plant plants as densely as possible in contaminated areas without affecting their normal growth.
对比现有技术,本发明的有益效果是:Compared with the prior art, the beneficial effects of the present invention are:
(1)本发明利用植物-微生物原位联合修复污染土壤和地下水,根际微域的微生物可提高根系活力和根系对污染物胁迫的耐受性,促进植物生长,而植物生长过程中根系产生的分泌物等,又促进了微生物的生长繁殖,两者相辅相成,可充分发挥植物与微生物的功能优势,提高污染土壤及地下水的修复效率。该系统成本低、效果好,绿色、清洁、无二次污染;(1) The present invention utilizes plant-microbe in situ joint restoration of polluted soil and groundwater, and the microorganisms in the rhizosphere micro-domain can improve root vitality and root tolerance to pollutant stress, and promote plant growth. The secretions, etc., promote the growth and reproduction of microorganisms, and the two complement each other, which can give full play to the functional advantages of plants and microorganisms, and improve the remediation efficiency of polluted soil and groundwater. The system has low cost, good effect, green, clean and no secondary pollution;
(2)在植物、微生物生长过程中,通过营养物质注射井、氧气注入井,给植物、微生物提供营养与氧气,促进植物快速生长、微生物的迅速繁殖,有效提高修复效率;(2) During the growth process of plants and microorganisms, nutrients and oxygen are provided to plants and microorganisms through nutrient injection wells and oxygen injection wells, so as to promote the rapid growth of plants and the rapid reproduction of microorganisms, and effectively improve the repair efficiency;
(3)在植物根系四周使用了聚内交酯限制层,驱使植物根系向深层的污染区域生长,能满足深层土壤及地下水污染修复的需要。且该系统采用原位修复方式,避免了因单纯采用抽出-处理技术而造成污染物残留引起的拖尾效应。(3) A polylactide restriction layer is used around the plant root system, which drives the plant root system to grow to the deep polluted area, which can meet the needs of deep soil and groundwater pollution remediation. Moreover, the system adopts the in-situ repair method, which avoids the tailing effect caused by the residual pollutants caused by the simple extraction-processing technology.
(4)在植物的根部设置下游抽水井,通过隔膜泵,将下游污染地下水抽提至上游注水井中,利用地下水流,将土壤及地下水中污染物带至植物根系处,可提高土壤及地下水中污染物与植物根系及微生物的接触机率、增加地下水曝气,加速有机污染降解,避免污染羽向下游扩散,从而可实现包气带污染土壤、饱和带污染土壤及地下水的协同修复。(4) Set up a downstream pumping well at the root of the plant, through the diaphragm pump, the downstream polluted groundwater is extracted to the upstream injection well, and the groundwater flow is used to bring the pollutants in the soil and groundwater to the plant root system, which can improve soil and groundwater. The contact probability of pollutants with plant roots and microorganisms is increased, groundwater aeration is increased, the degradation of organic pollution is accelerated, and pollution plumes are prevented from spreading downstream, so as to realize the coordinated restoration of vadose-contaminated soil, saturated-contaminated soil and groundwater.
附图说明Description of drawings
附图1是本发明结构示意图;Accompanying drawing 1 is the structural representation of the present invention;
附图2是营养物质注射井与氧气注入井结构示意图;Accompanying drawing 2 is the structural schematic diagram of nutrient injection well and oxygen injection well;
附图中所示标号:1、树叶;2、上游注水井;3、下游抽水井;4、聚内交酯限制层;5、隔膜泵;6、营养物质注射井;7、氧气注入井;8、养物质及微生物填充区;9、未受污染土壤;10、污染土壤及地下水;11、地下水流向;12、隔水层;13、树根;14、注射孔;15、筛孔。Numerals shown in the drawings: 1, leaves; 2, upstream water injection well; 3, downstream pumping well; 4, polylactide restriction layer; 5, diaphragm pump; 6, nutrient injection well; 7, oxygen injection well; 8. Nutrient and microbial filling area; 9. Uncontaminated soil; 10. Contaminated soil and groundwater; 11. Groundwater flow direction; 12. Water barrier; 13. Tree root; 14. Injection hole;
具体实施方式Detailed ways
结合附图和具体实施例,对本发明作进一步说明。应理解,这些实施例仅用于说明本发明而不用于限制本发明的范围。此外应理解,在阅读了本发明讲授的内容之后,本领域技术人员可以对本发明作各种改动或修改,这些等价形式同样落于本申请所限定的范围。The present invention will be further described with reference to the accompanying drawings and specific embodiments. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the teaching content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the present application.
如图1-2所示,本发明所述一种植物-微生物综合修复污染土壤及地下水系统,包括上游注水井2,下游抽水井3,隔膜泵5,隔水层12,所述下游抽水井3下端设有养物质及微生物填充区8,所述养物质及微生物填充区8位于地下水位±0.5m,且不穿透隔水层12,所述下游抽水井3四周设置聚内交酯限制层4、营养物质注射井6与氧气注入井7,所述聚内交酯限制层4顶部与地面平齐,所述聚内交酯限制层4具有防腐、防渗的作用,能够限制植物根部的生长,驱使植物根系向污染区域生长,所述营养物质注射井6上开设注射孔14、所述氧气注入井7下方开设筛孔15,筛孔15便于氧气渗入植物根系,待植物具有独立存活能力后,使用隔膜泵5,将下游污染地下水抽提至上游注水井2中,通过地下水流,将污染土壤及地下水中的污染物带至植物根系处。As shown in Figures 1-2, a plant-microorganism comprehensive remediation system for polluted soil and groundwater according to the present invention includes an upstream injection well 2, a downstream pumping well 3, a diaphragm pump 5, an
具体的,所述养物质及微生物填充区8内采用放线菌密旋链霉菌Act12与生物炭作为填充物料。Act12是一种广谱的促生菌,可刺激植物根系发育及养分吸收,调整植物根域土壤微生态平衡,抑制有害微生物的生长,促进有益微生物的繁殖,改善微生物区系,能够提高根系活力和根系对污染物胁迫的耐受性,提高处理负荷;优化根基环境,促进植物根系发展,增强植物修复治理重金属污染土壤的能力。Specifically, the nutrient and microorganism filling area 8 uses actinomycetes Streptomyces triceps Act12 and biochar as filling materials. Act12 is a broad-spectrum growth-promoting bacteria that can stimulate plant root development and nutrient absorption, adjust the soil micro-ecological balance of plant roots, inhibit the growth of harmful microorganisms, promote the reproduction of beneficial microorganisms, improve microbial flora, and improve root vitality. and root tolerance to pollutant stress, increase the treatment load; optimize the root environment, promote the development of plant roots, and enhance the ability of phytoremediation to treat heavy metal-contaminated soil.
具体的,所述下游抽水井3内种植植物。植物可通过植物提取、挥发、过滤、降解、等作用修复土壤及地下水中的污染物,同时植物根系巨大的表面积可为微生物提供寄宿之处,植物向根系输送的氧气和释放的根系分泌物,可使根际微域中土壤养分、酶活性、湿度等产生相应变化,从而促进微生物的生长及活性。Specifically, plants are grown in the downstream pumping well 3 . Plants can remediate pollutants in soil and groundwater through plant extraction, volatilization, filtration, degradation, etc. At the same time, the huge surface area of plant roots can provide a host for microorganisms, and the oxygen transported by plants to the roots and the released root secretions, It can make corresponding changes in soil nutrients, enzyme activity and humidity in the rhizosphere micro-domain, thereby promoting the growth and activity of microorganisms.
具体的,所述植物为桦树。桦树具有较大的重金属(如锌、镉、铅、镍、砷、铬、汞、硒和铜等)吸收潜力,且可修复有机污染土壤,又可以产生大量的木材或生物质能源。Specifically, the plant is birch. Birch has a great potential to absorb heavy metals (such as zinc, cadmium, lead, nickel, arsenic, chromium, mercury, selenium and copper, etc.), and can repair organically polluted soil, and can also generate a large amount of wood or biomass energy.
具体的,所述筛孔15外周设有3-4层纱网。纱网能够有效防止土壤颗粒物堵塞筛孔。Specifically, the
具体的,在不影响植物正常生长的前提下,在污染区域尽可能密集地种植植物。Specifically, under the premise of not affecting the normal growth of plants, plants are planted as densely as possible in the polluted area.
实施例:Example:
以桦树为修复植物,进行如下试验。Using birch as the restoration plant, the following experiments were carried out.
一种以植物-微生物联合修复污染地下水的修复体系,具体包括如下步骤:A remediation system for jointly remediating polluted groundwater with plants and microorganisms specifically includes the following steps:
S1:以污染源为中心,对地下水中污染物的种类、浓度、污染深度及地下水的流向进行调查;S1: Taking the pollution source as the center, investigate the type, concentration, pollution depth and groundwater flow direction of the pollutants in the groundwater;
S2:选择地下水污染深度为10-15m的污染点位,用大直径螺旋钻技术开挖直径为1m,深度位于污染点位地下水位±0.5m的井;S2: Select a pollution point with a groundwater pollution depth of 10-15m, and use the large-diameter auger technology to excavate a well with a diameter of 1m and a depth of ±0.5m from the groundwater level of the pollution point;
S3:使用水平钻工艺在污染区域开挖通道,将植物生长所需的营养成分以及微生物(密旋链霉菌Act12和生物炭),放置于通道内,形成养物质及微生物填充区;S3: Use the horizontal drilling process to excavate a channel in the polluted area, and place the nutrients and microorganisms (Streptomyces triceps Act12 and biochar) required for plant growth in the channel to form a filling area with nutrients and microorganisms;
S4:在井的四周设置聚内交酯限制层,贴着井内部,顶部与地面平齐,在井的四周设置营养物质注射井、氧气注入井。S4: Set a polylactide restriction layer around the well, close to the inside of the well, the top is flush with the ground, and set up nutrient injection wells and oxygen injection wells around the well.
S5:在井内种植3年以上树龄的桦树,在污染区域以上述方式种植桦树,树矩为10m;S5: Plant birch trees over 3 years old in the well, and plant birch trees in the polluted area in the above manner, with a tree length of 10m;
S6:在桦树移栽后的初期,每周一次向根际浇水,每月一次通过营养物质注入井、氧气注入井注射营养物质及氧气,直至桦树能够正常生长;S6: In the early stage after the birch is transplanted, water the rhizosphere once a week, and inject nutrients and oxygen through the nutrient injection well and oxygen injection well once a month until the birch can grow normally;
S7:在植物根部设置下游抽水井,并在上游设置隔膜泵和上游注水井,使使用隔膜泵将下游受污染地下水抽至上游注水井,利用地下水流向,将下游地下水引至植物根部,利用植物-微生物综合修复系统对土壤和地下水进行修复。S7: Set up a downstream pumping well at the root of the plant, and set up a diaphragm pump and an upstream water injection well in the upstream, so that the diaphragm pump is used to pump the downstream contaminated groundwater to the upstream injection well, and the groundwater flow direction is used to lead the downstream groundwater to the plant roots, and use the plant - Microbial integrated remediation system to remediate soil and groundwater.
S8:检测各组织中的污染物含量及地下水中各污染物的浓度,由此判断桦树的修复情况。S8: Detect the pollutant content in each tissue and the concentration of each pollutant in the groundwater, thereby judging the restoration of birch trees.
每隔三个月,在地下水污染区域用抽提泵抽提地下水进行定期检测,检测数据显示,在桦树种植后的第四年,地下水污染物浓度达到《地下水质量标准(GB/T14848-2017)》Ⅳ类水标准(如表1)。此时,取种植四年的桦树的根、茎、叶组织进行微波消解,用ICP-AES方法测定各组织器官中的污染物含量,结果如表2、表3所示。Every three months, the groundwater is extracted with an extraction pump in the groundwater polluted area for regular testing. The test data shows that in the fourth year after the birch is planted, the concentration of groundwater pollutants has reached the "Groundwater Quality Standard (GB/T14848-2017"). ) "Class IV water standard (as shown in Table 1). At this time, the roots, stems and leaves of birch trees planted for four years were taken for microwave digestion, and the pollutant content in each tissue and organ was determined by ICP-AES method. The results are shown in Table 2 and Table 3.
表1 地下水污染物浓度(修复前)Table 1 Concentration of groundwater pollutants (before restoration)
表1Table 1
表2 地下水污染物浓度(修复后)Table 2 Concentration of groundwater pollutants (after restoration)
表2Table 2
表3桦树各器官浓度(修复后)Table 3 The concentration of each organ of birch (after repair)
表3table 3
从上述结果中可以看出,桦树对地下水中的重金属及有机物有较好的修复效果,且在各器官中的积累量各不相同。植物主要通过植物根系过滤、植物挥发、根际降解及与根际微生物的协同作用,共同修复污染地下水。It can be seen from the above results that birch has a good remediation effect on heavy metals and organic matter in groundwater, and the accumulation amount in each organ is different. Plants remediate polluted groundwater mainly through plant root filtration, plant volatilization, rhizosphere degradation, and synergy with rhizosphere microorganisms.
最后应说明的是:以上实施例仅用以说明本发明的技术方案,而非对其限制;尽管参照前述实施例对本发明进行了详细的说明,本领域的普通技术人员应当理解:其依然可以对前述各实施例所记载的技术方案进行修改,或者对其中部分技术特征进行等同替换;而这些修改或者替换,并不使相应技术方案的本质脱离本发明各实施例技术方案的精神和范围。Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand: it can still be Modifications are made to the technical solutions described in the foregoing embodiments, or some technical features thereof are equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
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