CN111302840A - Triple fermentation process method of pure plant source organic fertilizer - Google Patents

Triple fermentation process method of pure plant source organic fertilizer Download PDF

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Publication number
CN111302840A
CN111302840A CN202010283289.8A CN202010283289A CN111302840A CN 111302840 A CN111302840 A CN 111302840A CN 202010283289 A CN202010283289 A CN 202010283289A CN 111302840 A CN111302840 A CN 111302840A
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fermentation
organic fertilizer
pure plant
plant source
triple
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邓永议
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Jiangsu Ladybug Biotechnology Co ltd
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Jiangsu Ladybug Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/006Waste from chemical processing of material, e.g. diestillation, roasting, cooking
    • C05F5/008Waste from biochemical processing of material, e.g. fermentation, breweries
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F7/00Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023Methane
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/30Fuel from waste, e.g. synthetic alcohol or diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention relates to a triple fermentation process method of a pure plant source organic fertilizer, which is characterized by comprising the following steps: step S1, preparing raw materials; step S2, fermenting with yeast for the first time; step S3, carrying out second anaerobic fermentation; and step S4, performing high-temperature aerobic fermentation for the third time. The invention also discloses a pure plant source organic fertilizer produced by the triple fermentation process method of the pure plant source organic fertilizer. The triple fermentation process method of the pure plant source organic fertilizer disclosed by the invention is simple to operate, low in cost, small in occupied space, high in fermentation speed and good in effect, and after three times of fermentation, complex macromolecular organic matters are fully degraded into simple micromolecular organic matters, so that the absorption and utilization of soil microorganisms and plants are facilitated; when the prepared organic fertilizer is applied, the fertilizer does not need to be isolated from crop roots, the roots can be in direct contact with the fertilizer, the more the roots are in contact with the fertilizer, the better the growth promoting effect is.

Description

Triple fermentation process method of pure plant source organic fertilizer
Technical Field
The invention relates to the technical field of organic fertilizers, in particular to a triple fermentation process method of a pure plant source organic fertilizer.
Background
The yield of crops can be ensured only by applying fertilizers at regular time in the growth process, and the current agricultural application mainly adopts chemical fertilizers which have the effect of increasing the yield, but the large amount of the chemical fertilizers can cause serious damage to soil, such as environmental pollution, element imbalance, soil hardening and the like. The organic fertilizer can relieve the problems to a great extent, the raw materials of the organic fertilizer are cheap and easy to obtain, and the organic fertilizer generally uses substances with higher organic matters, such as livestock and poultry manure, agricultural product waste, garbage and the like. The organic fertilizer can improve the soil environment and improve the utilization efficiency of agricultural and sideline products, is a green and environment-friendly fertilizer and plays an increasingly important role in agricultural production.
The organic fertilizer is obtained by utilizing various organic substances in the nature and fermenting through microbial treatment. The production of organic fertilizer must be through the process of compost fermentation. Composting fermentation is a process in which organic matter is continuously degraded and stabilized under the action of microorganisms under certain conditions and a product suitable for land utilization is produced. Although the existing organic fertilizer fermentation has large scale of fermentation compost treatment, low cost and good fertilizer preparation efficiency, the existing organic fertilizer fermentation has the defects of low fermentation speed, large occupied area, poor treatment site environment, continuous turning by external force in the fermentation process and the like, and is difficult to automatically dry in the fermentation process. In addition, the fermentation method of the organic fertilizer in the prior art is complex, the fermentation time is long, and the prepared organic fertilizer has insufficient fertility and poor crop absorption.
The Chinese patent with application number 201510065924.4 discloses an organic fertilizer fermentation method, which comprises the steps of preparing fermentation bacteria for mixing, turning over organic fertilizers for fermentation, managing fermentation and the like, wherein the fermentation bacteria adopt spore bacteria, and the spore bacteria are high-temperature resistant and can generate a large amount of heat in the fermentation process, so that ova and germs in excrement are killed, and the excrement is dried, and the excrement temperature in the fermentation process reaches 90-100 ℃, so that organic matters in the excrement cannot be carbonized, the organic matter content in the organic fertilizer is higher, and the finished product is rich in nutrition. However, the organic fertilizer obtained by the fermentation method has limited improvement degree on organic matters and humus, and the growth promoting effect is required to be further improved.
Therefore, it is important to find a more suitable organic fertilizer fermentation method.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a triple fermentation process method of a pure plant source organic fertilizer, the method has the advantages of simple operation, low cost, small occupied space, high fermentation speed and good effect, and after three times of fermentation, complex macromolecular organic matters are fully degraded into simple micromolecular organic matters, so that the absorption and utilization of soil microorganisms and plants are facilitated; when the prepared organic fertilizer is applied, the fertilizer does not need to be isolated from crop roots, the roots can be in direct contact with the fertilizer, the more the roots are in contact with the fertilizer, the better the growth promoting effect is.
In order to achieve the purpose, the invention adopts the technical scheme that: a triple fermentation process method of a pure plant source organic fertilizer is characterized by comprising the following steps:
step S1, raw material preparation: sequentially dehydrating and crushing cassava, and sieving with a 50-100 mesh sieve to obtain cassava powder;
step S2, first yeast fermentation: mixing the cassava powder prepared in the step S1 with a liquefying enzyme, adding the mixture into a liquefier for liquefying for 1-2 days, transferring the liquefied product into a saccharifying tank, adding the saccharifying enzyme, carrying out saccharification treatment at the temperature of 100 ℃ and 110 ℃ for 2-4 days, adding the saccharified product into a fermentation tank, inoculating Dangbaoli high-temperature resistant active dry yeast powder into the fermentation tank, fermenting for 1-2 days, and extracting ethanol through a rough distillation tower, a rectifying tower, a condenser and a molecular sieve adsorption tower in sequence during the fermentation process to obtain a byproduct cassava residue;
step S3, second anaerobic fermentation: mixing the byproduct cassava residue prepared in the step S2 with water, and then inoculating methanobacterium to obtain an anaerobic fermentation material; then adding the anaerobic fermentation material into a high-temperature anaerobic fermentation tank for high-temperature anaerobic fermentation for 2-3 days, storing gas generated in the fermentation process in a methane storage tank, obtaining a fermentation product through a settling tank, and passing the fermentation product through an extrusion type solid-liquid separation system to obtain biogas slurry and fermented cassava residue;
step S4, performing high-temperature aerobic fermentation for the third time: and (4) inoculating the fermented cassava residues prepared in the step (S3) with a mixed microbial inoculum, and carrying out high-temperature aerobic fermentation with the assistance of a loader, a turner and conveying and packaging system equipment to obtain the pure plant source organic fertilizer.
Preferably, the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the danbaoli high-temperature resistant active dry yeast powder in the step S2 is (500-.
Preferably, the mass ratio of the byproduct cassava residue, water and the methanobacterium in the step S3 is 1 (4-6) to (0.001-0.002).
Preferably, the temperature of the high-temperature anaerobic fermentation is 60-70 ℃.
Preferably, the mass ratio of the cassava residue after fermentation and the mixed microbial inoculum in the step S4 is (500- & ltSUB & gt 600- & gt) 1.
Preferably, the mixed microbial inoculum comprises an organic fertilizer decomposing agent, bacillus subtilis and bacillus licheniformis.
Preferably, the mass ratio of the organic fertilizer decomposing inoculant to the bacillus subtilis to the bacillus licheniformis is 1:0.3: 0.5.
Preferably, the high-temperature aerobic fermentation is carried out at the temperature of 60-70 ℃ for 20-30 days.
The invention also aims to provide a pure plant source organic fertilizer produced by the triple fermentation process method of the pure plant source organic fertilizer.
Further, the pure plant source organic fertilizer is one of a powder product or a particle product.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages: the triple fermentation process method of the pure plant source organic fertilizer provided by the invention has the advantages of simple operation, low cost, small occupied space, high fermentation speed and good effect, and after three times of fermentation, complex macromolecular organic matters are fully degraded into simple micromolecular organic matters, so that the triple fermentation process method is more beneficial to the absorption and utilization of soil microorganisms and plants; when the prepared organic fertilizer is applied, the fertilizer does not need to be isolated from crop roots, the roots can be in direct contact with the fertilizer, the more the roots are in contact with the fertilizer, the better the growth promoting effect is.
Detailed Description
A triple fermentation process method of a pure plant source organic fertilizer is characterized by comprising the following steps:
step S1, raw material preparation: sequentially dehydrating and crushing cassava, and sieving with a 50-100 mesh sieve to obtain cassava powder;
step S2, first yeast fermentation: mixing the cassava powder prepared in the step S1 with a liquefying enzyme, adding the mixture into a liquefier for liquefying for 1-2 days, transferring the liquefied product into a saccharifying tank, adding the saccharifying enzyme, carrying out saccharification treatment at the temperature of 100 ℃ and 110 ℃ for 2-4 days, adding the saccharified product into a fermentation tank, inoculating Dangbaoli high-temperature resistant active dry yeast powder into the fermentation tank, fermenting for 1-2 days, and extracting ethanol through a rough distillation tower, a rectifying tower, a condenser and a molecular sieve adsorption tower in sequence during the fermentation process to obtain a byproduct cassava residue;
step S3, second anaerobic fermentation: mixing the byproduct cassava residue prepared in the step S2 with water, and then inoculating methanobacterium to obtain an anaerobic fermentation material; then adding the anaerobic fermentation material into a high-temperature anaerobic fermentation tank for high-temperature anaerobic fermentation for 2-3 days, storing gas generated in the fermentation process in a methane storage tank, obtaining a fermentation product through a settling tank, and passing the fermentation product through an extrusion type solid-liquid separation system to obtain biogas slurry and fermented cassava residue;
step S4, performing high-temperature aerobic fermentation for the third time: and (4) inoculating the fermented cassava residues prepared in the step (S3) with a mixed microbial inoculum, and carrying out high-temperature aerobic fermentation with the assistance of a loader, a turner and conveying and packaging system equipment to obtain the pure plant source organic fertilizer.
Preferably, the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the danbaoli high-temperature resistant active dry yeast powder in the step S2 is (500-; in the step S3, the mass ratio of the byproduct manioc waste, water and methanobacterium is 1 (4-6) to 0.001-0.002; the temperature of the high-temperature anaerobic fermentation is 60-70 ℃; the mass ratio of the fermented cassava residues to the mixed microbial inoculum in the step S4 is (500) -600) to 1; the mixed microbial inoculum comprises an organic fertilizer decomposing agent, bacillus subtilis and bacillus licheniformis; the mass ratio of the organic fertilizer decomposing inoculant to the bacillus subtilis to the bacillus licheniformis is 1:0.3: 0.5.
Preferably, the high-temperature aerobic fermentation is carried out at the temperature of 60-70 ℃ for 20-30 days.
The invention also aims to provide a pure plant source organic fertilizer produced by the triple fermentation process method of the pure plant source organic fertilizer.
Further, the pure plant source organic fertilizer is one of a powder product or a particle product.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages: the triple fermentation process method of the pure plant source organic fertilizer provided by the invention has the advantages of simple operation, low cost, small occupied space, high fermentation speed and good effect, and after three times of fermentation, complex macromolecular organic matters are fully degraded into simple micromolecular organic matters, so that the triple fermentation process method is more beneficial to the absorption and utilization of soil microorganisms and plants; when the prepared organic fertilizer is applied, the fertilizer does not need to be isolated from crop roots, the roots can be in direct contact with the fertilizer, the more the roots are in contact with the fertilizer, the better the growth promoting effect is.
The invention will be further described with reference to specific examples, but the scope of protection of the invention is not limited thereto:
example 1
The embodiment 1 provides a pure plant source organic fertilizer triple fermentation process method, which is characterized by comprising the following steps:
step S1, raw material preparation: sequentially dehydrating and crushing cassava, and sieving with a 50-mesh sieve to obtain cassava powder;
step S2, first yeast fermentation: mixing the cassava flour prepared in the step S1 with a liquefying enzyme, adding the mixture into a liquefier for liquefying for 1 day, transferring a liquefied product into a saccharifying tank, adding the saccharifying enzyme, carrying out saccharification treatment at 100 ℃ for 2 days, adding the saccharified product into a fermentation tank, inoculating Dandeli high-temperature resistant active dry yeast powder into the fermentation tank, fermenting for 1 day, and extracting the ethanol through a coarse distillation tower, a rectification tower, a condenser and a molecular sieve adsorption tower in sequence during the fermentation process to obtain a byproduct cassava residue;
step S3, second anaerobic fermentation: mixing the byproduct cassava residue prepared in the step S2 with water, and then inoculating methanobacterium to obtain an anaerobic fermentation material; then adding the anaerobic fermentation material into a high-temperature anaerobic fermentation tank for high-temperature anaerobic fermentation for 2 days, storing gas generated in the fermentation process in a methane storage tank, obtaining a fermentation product through a settling tank, and passing the fermentation product through an extrusion type solid-liquid separation system to obtain biogas slurry and fermented cassava residues;
step S4, performing high-temperature aerobic fermentation for the third time: and (4) inoculating the fermented cassava residues prepared in the step (S3) with a mixed microbial inoculum, and carrying out high-temperature aerobic fermentation with the assistance of a loader, a turner and conveying and packaging system equipment to obtain the pure plant source organic fertilizer.
In the step S2, the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the danbao li high-temperature resistant active dry yeast powder is 500:1:1: 1.
The mass ratio of the byproduct cassava residue, water and the methanobacterium in the step S3 is 1:4: 0.001.
The temperature of the high-temperature anaerobic fermentation is 60 ℃.
The mass ratio of the fermented cassava residue to the mixed microbial inoculum in the step S4 is 500: 1.
The mixed microbial inoculum comprises an organic fertilizer decomposing agent, bacillus subtilis and bacillus licheniformis; the mass ratio of the organic fertilizer decomposing inoculant to the bacillus subtilis to the bacillus licheniformis is 1:0.3: 0.5.
The temperature of the high-temperature aerobic fermentation is 60 ℃, and the fermentation time is 20 days.
A pure plant source organic fertilizer produced according to the triple fermentation process method of the pure plant source organic fertilizer; the pure plant source organic fertilizer is a powder product.
Example 2
Embodiment 2 provides a triple fermentation process method of a pure plant source organic fertilizer, which is basically the same as embodiment 1, except that in step S2, the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the danbao li high temperature resistant active dry yeast powder is 520:1:1: 1.2; the mass ratio of the byproduct cassava residue, water and the methanobacterium in the step S3 is 1:4.5: 0.0013; the temperature of the high-temperature anaerobic fermentation is 63 ℃; the mass ratio of the fermented cassava residue to the mixed microbial inoculum in the step S4 is 530: 1.
Example 3
Embodiment 3 provides a triple fermentation process method of a pure plant source organic fertilizer, which is basically the same as embodiment 1, except that the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the danbao li high-temperature resistant active dry yeast powder in the step S2 is 550:1:1: 1.5; the mass ratio of the byproduct cassava residue, water and the methanobacterium in the step S3 is 1:5: 0.0015; the temperature of the high-temperature anaerobic fermentation is 65 ℃; the mass ratio of the fermented cassava residue to the mixed microbial inoculum in the step S4 is 550: 1.
Example 4
Embodiment 4 provides a triple fermentation process method of a pure plant source organic fertilizer, which is basically the same as embodiment 1, except that in step S2, the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the danbao li high temperature resistant active dry yeast powder is 580:1:1: 1.8; the mass ratio of the byproduct cassava residue, water and the methanobacterium in the step S3 is 1:5.5: 0.0018; the temperature of the high-temperature anaerobic fermentation is 68 ℃; the mass ratio of the fermented cassava residue to the mixed microbial inoculum in the step S4 is 585: 1.
Example 5
Embodiment 5 provides a triple fermentation process method of a pure plant source organic fertilizer, which is basically the same as embodiment 1, except that the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the danbao li high-temperature resistant active dry yeast powder in the step S2 is 600:1:1: 2; the mass ratio of the byproduct cassava residue, water and the methanobacterium in the step S3 is 1:6: 0.002; the temperature of the high-temperature anaerobic fermentation is 70 ℃; the mass ratio of the fermented cassava residue to the mixed microbial inoculum in the step S4 is 600: 1.
Comparative example 1
Comparative example 1 provides a triple fermentation process of a pure plant-derived organic fertilizer, which is substantially the same as example 1 except that there is no first yeast fermentation of step S2.
Comparative example 2
Comparative example 2 provides a triple fermentation process of a pure plant-derived organic fertilizer, which is substantially the same as example 1 except that there is no second anaerobic fermentation of step S3.
Comparative example 3
Comparative example 3 provides a triple fermentation process of a pure plant source organic fertilizer, which is basically the same as that in example 1, except that the mixed microbial inoculum does not include an organic fertilizer decomposing agent.
In order to further illustrate the beneficial technical effects of the embodiments, the fertilizer efficiency of the pure plant source organic fertilizer produced by the triple fermentation process method of the pure plant source organic fertilizer in each of the embodiments 1 to 5 and the comparative examples 1 to 3 is tested, and the results are shown in table 1, and the test method is as follows: after the organic grape fruits are picked, digging holes with the depth of 40cm and the diameter of 40cm close to the original grape tree planting holes outwards, and applying various fertilizers for several times according to a fertilizing mode of one layer of soil and one layer of fertilizer, wherein the using amount of the fertilizer is 1800 kg/mu. After fertilization, respectively measuring the organic matter content (333 mmol/LKMNO) in the corresponding soil when the organic grapes are ripe in July of the next year4Oxidation colorimetry measurement) and the percent yield increase is calculated based on the yield of organic grapes (compared with applying a commercial common compound fertilizer as a benchmark).
As can be seen from Table 1, the pure plant-derived organic fertilizer produced by the triple fermentation process of the pure plant-derived organic fertilizer of examples 1 to 5 has soil improvement and yield increase effects significantly superior to those of the comparative example, which are the result of synergistic effects of the components.
TABLE 1
Test items Organic matter content of soil Increase production
Unit of g/kg
Example 1 12.3 19.5
Example 2 12.5 19.9
Example 3 12.6 20.4
Example 4 13.0 20.8
Example 5 13.4 21.3
Comparative example 1 9.8 15.4
Comparative example 2 10.2 15.0
Comparative example 3 11.2 15.2
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (10)

1. A triple fermentation process method of a pure plant source organic fertilizer is characterized by comprising the following steps:
step S1, raw material preparation: sequentially dehydrating and crushing cassava, and sieving with a 50-100 mesh sieve to obtain cassava powder;
step S2, first yeast fermentation: mixing the cassava powder prepared in the step S1 with a liquefying enzyme, adding the mixture into a liquefier for liquefying for 1-2 days, transferring the liquefied product into a saccharifying tank, adding the saccharifying enzyme, carrying out saccharification treatment at the temperature of 100 ℃ and 110 ℃ for 2-4 days, adding the saccharified product into a fermentation tank, inoculating Dangbaoli high-temperature resistant active dry yeast powder into the fermentation tank, fermenting for 1-2 days, and extracting ethanol through a rough distillation tower, a rectifying tower, a condenser and a molecular sieve adsorption tower in sequence during the fermentation process to obtain a byproduct cassava residue;
step S3, second anaerobic fermentation: mixing the byproduct cassava residue prepared in the step S2 with water, and then inoculating methanobacterium to obtain an anaerobic fermentation material; then adding the anaerobic fermentation material into a high-temperature anaerobic fermentation tank for high-temperature anaerobic fermentation for 2-3 days, storing gas generated in the fermentation process in a methane storage tank, obtaining a fermentation product through a settling tank, and passing the fermentation product through an extrusion type solid-liquid separation system to obtain biogas slurry and fermented cassava residue;
step S4, performing high-temperature aerobic fermentation for the third time: and (4) inoculating the fermented cassava residues prepared in the step (S3) with a mixed microbial inoculum, and carrying out high-temperature aerobic fermentation with the assistance of a loader, a turner and conveying and packaging system equipment to obtain the pure plant source organic fertilizer.
2. The triple fermentation process method of pure plant source organic fertilizer as claimed in claim 1, wherein the mass ratio of the cassava powder, the liquefying enzyme, the saccharifying enzyme and the Danbao Li high temperature resistant active dry yeast powder in step S2 is (500) -600: 1 (1-2).
3. The triple fermentation process of pure plant source organic fertilizer as claimed in claim 1, wherein the mass ratio of the byproduct cassava residue, water and methanobacterium in step S3 is 1 (4-6): 0.001-0.002).
4. The triple fermentation process method of pure plant source organic fertilizer as claimed in claim 1, wherein the temperature of the high temperature anaerobic fermentation is 60-70 ℃.
5. The triple fermentation process method of pure plant source organic fertilizer as claimed in claim 1, wherein the mass ratio of the fermented cassava residue to the mixed microbial inoculum in step S4 is (500- & gt 600) & gt 1.
6. The triple fermentation process method of pure plant source organic fertilizer as claimed in claim 1, wherein the mixed microbial inoculum comprises organic fertilizer decomposing agent, bacillus subtilis and bacillus licheniformis.
7. The triple fermentation process method of the pure plant source organic fertilizer as claimed in claim 6, wherein the mass ratio of the organic fertilizer decomposing agent to the bacillus subtilis to the bacillus licheniformis is 1:0.3: 0.5.
8. The triple fermentation process method of pure plant source organic fertilizer as claimed in claim 1, wherein the temperature of the high temperature aerobic fermentation is 60-70 ℃, and the fermentation time is 20-30 days.
9. The pure plant source organic fertilizer produced by the triple fermentation process method of any one of claims 1 to 8.
10. The triple fermentation process of pure plant-source organic fertilizer as claimed in any one of claims 1-8, wherein the pure plant-source organic fertilizer is one of powder product or granular product.
CN202010283289.8A 2020-04-13 2020-04-13 Triple fermentation process method of pure plant source organic fertilizer Withdrawn CN111302840A (en)

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Application publication date: 20200619