CN111073997A - Kit for early and rapid screening of HPV16-E6 and HPV18-E6 - Google Patents
Kit for early and rapid screening of HPV16-E6 and HPV18-E6 Download PDFInfo
- Publication number
- CN111073997A CN111073997A CN201811221195.7A CN201811221195A CN111073997A CN 111073997 A CN111073997 A CN 111073997A CN 201811221195 A CN201811221195 A CN 201811221195A CN 111073997 A CN111073997 A CN 111073997A
- Authority
- CN
- China
- Prior art keywords
- hpv16
- hpv18
- kit
- detection
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012216 screening Methods 0.000 title claims abstract description 13
- 101100540311 Human papillomavirus type 16 E6 gene Proteins 0.000 title claims abstract description 10
- 101000954519 Human papillomavirus type 18 Protein E6 Proteins 0.000 title claims abstract description 9
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 16
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 16
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 16
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 241000341655 Human papillomavirus type 16 Species 0.000 claims abstract description 4
- 238000005070 sampling Methods 0.000 claims abstract 4
- 239000006166 lysate Substances 0.000 claims abstract 2
- 229920000742 Cotton Polymers 0.000 claims description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- 229960002685 biotin Drugs 0.000 claims description 3
- 235000020958 biotin Nutrition 0.000 claims description 3
- 239000011616 biotin Substances 0.000 claims description 3
- 238000005336 cracking Methods 0.000 claims description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 claims description 2
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 claims description 2
- 229960005156 digoxin Drugs 0.000 claims description 2
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims 2
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 17
- 238000000034 method Methods 0.000 abstract description 3
- 238000011529 RT qPCR Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 10
- 230000003321 amplification Effects 0.000 description 5
- 230000009089 cytolysis Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 206010008342 Cervix carcinoma Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 2
- 201000010881 cervical cancer Diseases 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000003317 immunochromatography Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 210000004877 mucosa Anatomy 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010014594 Heterogeneous Nuclear Ribonucleoprotein A1 Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012123 point-of-care testing Methods 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kit for quickly screening HPV16-E6 and HPV18-E6 at an early stage. The kit for rapidly detecting HPV16 and HPV18 is prepared by preparing a nucleic acid lysate, a nucleic acid cleaning solution and a normal-temperature PCR reaction solution, and does not depend on instruments and professional operators. The current HPV detection method in the market mainly adopts a qPCR method, the requirements on personnel and instruments are very high, a cervical sieve is needed during sampling, and medical personnel must be used for assisting sampling. The invention can rapidly detect HPV, is convenient for sampling and detection, can complete self-detection at home after being purchased by a user, and is particularly suitable for home detection and township hospitals lacking detection equipment.
Description
Technical Field
The invention belongs to the field of biological detection, relates to a medical detection consumable, and particularly relates to a kit for quickly screening HPV16-E6 and HPV18-E6 at an early stage.
Background
HPV is a small DNA virus without cell membranes, and about 170 HPV subtypes are currently recognized. HPV is divided into low-risk type and high-risk type, and HPV16 and HPV18 are the two most main high-risk types. Studies have demonstrated that 99.7% of cervical cancers are due to long-term persistent infection with HPV.
According to the guidance suggestion about promoting the construction of the hierarchical diagnosis and treatment system issued by the office of the state institute, the hierarchical diagnosis and treatment mode of the first diagnosis, the two-way referral, the quick and slow treatment and the up-down linkage of the basic level is gradually formed in 2020, and the hierarchical diagnosis and treatment system according with the state condition is basically established. The outline of development of women in China (2011-Yi 2020) pointed out that the incidence of 'two cancers' in women in China is controlled to be lower before 2020. Therefore, the cervical cancer screening method has huge market space in China in the future. The HPV detection product meeting the large-scale screening requirement needs to meet the requirements of controllable cost and simple and convenient operation, and is preferably a product which can produce results in a short time by a single person, namely the HPV detection product of POCT, and the product is also suitable for clinical application and development.
At present, the HPV screening in China mainly adopts a fluorescent quantitative PCR method, the time consumption is too long for extracting nucleic acid and detecting samples, the requirements on the technical performance of operators and the accuracy of instruments and equipment are very high, and the screening detection work can be carried out only in a professional hospital or a third-party detection platform.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a kit for early and rapid screening of HPV16-E6 and HPV 18-E6. A rapid nucleic acid extraction method, a normal-temperature nucleic acid amplification system and an immunochromatography technology are adopted to prepare a convenient and rapid HPV16-E6 and HPV18-E6 detection kit, the product does not depend on any detection instrument, and a user can detect the HPV at home.
S1, using a cotton swab to scrape the vaginal mucosa to obtain a detection sample, and obtaining a nucleic acid sample after cracking and cleaning;
s2, placing the nucleic acid sample into a normal-temperature nucleic acid amplification system, and standing for 30 minutes at normal temperature to obtain a solution to be detected after PCR amplification;
s3, inserting the immunochromatographic test paper into the liquid to be detected, standing for 5 minutes, and observing the result.
Compared with the prior art, the invention has the following beneficial effects:
the invention adopts HPV16-E6 and HPV18-E6 special labeled primers and combines three rapid detection technologies, thereby achieving the purpose of screening high-risk HPV16 and HPV18 simply and rapidly without depending on instruments and technicians.
Drawings
Fig. 1 is an essential part of the present invention.
The main reference numbers illustrate:
1: a cotton swab; 2: filtering paper; 3: a test strip; 4: a lysis solution; 5: cleaning fluid; 6: and (3) PCR reaction liquid.
FIG. 2 is a flow chart of the detection of the kit of the present invention.
The main reference numbers illustrate:
1: a cotton swab; 2: filtering paper; 3: a test strip; 4: a lysis solution; 5: cleaning fluid; 6: and (3) PCR reaction liquid.
Detailed Description
The invention is further described in detail in the following with reference to the drawings and the detailed description of the invention, which are not intended to limit the invention in any way.
Example 1.
The rapid extraction of nucleic acid in a sample to be detected comprises the following steps:
s1, collecting samples
Scraping the vaginal mucosa with cotton swab for 6-10 times to obtain mucosal epithelial cells.
S2 extraction of nucleic acids
A cotton swab is put into a tube 1 lysis solution, the components of the lysis solution (20 mM Tris [ pH 8.0], 25 mM NaCl, 2.5mM EDTA, 0.05% SDS) are immersed into the lysis solution by a piece of filter paper to combine nucleic acid, the filter paper is put into a cleaning solution after 3 seconds, the components of the cleaning solution (10 mM Tris with pH 8.0, 0.1% Tween-20) are added into a normal-temperature nucleic acid amplification tube after 3 seconds, and the filter paper is put into the normal-temperature nucleic acid amplification tube after 3 seconds.
S3 Normal temperature PCR
The normal-temperature nucleic acid amplification system comprises the following components: HPV16E6-F1: 5 '-BIO-TATGCTGTATGTGATAAATG-3'),
HPV16E6-R1: 5'-DIG-TGTTCTAATGTTGTTCCA-3'、HPV18E6-F1: 5'- BIO-GGTGCCAGAAACCGTTGA-3'、HPV18E6-F1: 5'-DIG-CTGCGTCGTTGGAGTCGT-3'、
Recombinase, single-strand binding protein, strand displacement DNA polymerase, PCR MIX and double distilled water; the PCR reaction was carried out at 37 ℃ for 30 minutes.
S4 immunochromatography reaction detection sample
Adding the BIOTIN antibody into the colloidal gold solution, wherein the solubility is 10 micrograms per milliliter, and spraying the BIOTIN antibody on the bonding pad according to the dosage of 4 microliters per centimeter; the analytical membrane had two bands, the antibody concentration was 0.4 mg/ml, and the two bands were C and T, respectively, C was BIONTIN antibody and T was DIGOXIN antibody. After the sample pad, the conjugate pad, the analytical membrane, and the absorbent pad were sequentially affixed and fixed, the sample pad was inserted into the reaction solution of S3, and the results were read within 5 minutes.
Claims (3)
1. A kit for early and rapid screening of HPV16-E6 and HPV18-E6, which is characterized by comprising the following steps:
s1, sampling by a cotton swab, and quickly cracking in a cracking solution to obtain a nucleic acid sample;
s2, adsorbing the nucleic acid sample by filter paper, and washing in a washing solution;
s3, putting the filter paper into a rapid normal-temperature PCR tube to amplify HPV16 and 18;
s4, detecting the PCR product by using a colloidal gold test strip.
2. The kit for early rapid screening of HPV16-E6 and HPV18-E6 according to claim 1, wherein the upstream primer used in PCR is labeled with biotin and the downstream primer is labeled with digoxin.
3. The kit for early and rapid screening of HPV16-E6 and HPV18-E6 according to claim 1, which comprises a cotton swab, filter paper, a colloidal gold test strip, lysate, a cleaning solution and a normal temperature PCR reaction solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811221195.7A CN111073997A (en) | 2018-10-19 | 2018-10-19 | Kit for early and rapid screening of HPV16-E6 and HPV18-E6 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811221195.7A CN111073997A (en) | 2018-10-19 | 2018-10-19 | Kit for early and rapid screening of HPV16-E6 and HPV18-E6 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111073997A true CN111073997A (en) | 2020-04-28 |
Family
ID=70309164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811221195.7A Pending CN111073997A (en) | 2018-10-19 | 2018-10-19 | Kit for early and rapid screening of HPV16-E6 and HPV18-E6 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111073997A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113281510A (en) * | 2021-07-23 | 2021-08-20 | 深圳市中科先见医疗科技有限公司 | Antigen colloidal gold kit for rapid virus diagnosis |
| CN117074668A (en) * | 2022-10-31 | 2023-11-17 | 安徽艾赛尔智能科技有限公司 | HPV virus typing detection kit and preparation method thereof |
| CN117757991A (en) * | 2023-12-27 | 2024-03-26 | 海宁奥羚医学检验实验室有限公司 | POCT detection method for DNA and mRNA of HPV16/18 virus gene in cervical exfoliated cells |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104561384A (en) * | 2015-01-19 | 2015-04-29 | 南京爱必梦生物材料有限公司 | HPV detection kit |
| US20160076069A1 (en) * | 2013-03-15 | 2016-03-17 | Theranos, Inc. | Nucleic Acid Amplification |
| CN107177684A (en) * | 2017-06-15 | 2017-09-19 | 刘琳 | A kind of constant temperature nucleic acid amplification reaction reagent |
| CN107177699A (en) * | 2017-05-10 | 2017-09-19 | 宝瑞源生物技术(北京)有限公司 | A kind of human papilloma virus（HPV）Parting quick determination method |
| CN109628646A (en) * | 2019-01-24 | 2019-04-16 | 郑筱玮 | A kind of detection method of the E6 or E7 nucleic acid of HPV16 or HPV18 virus based on RPA |
| CN109943663A (en) * | 2019-03-22 | 2019-06-28 | 安徽深蓝医疗科技股份有限公司 | For recombinase amplification detection method, test strips and its application of 18 genotype of HPV 16 and HPV |
-
2018
- 2018-10-19 CN CN201811221195.7A patent/CN111073997A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160076069A1 (en) * | 2013-03-15 | 2016-03-17 | Theranos, Inc. | Nucleic Acid Amplification |
| CN104561384A (en) * | 2015-01-19 | 2015-04-29 | 南京爱必梦生物材料有限公司 | HPV detection kit |
| CN107177699A (en) * | 2017-05-10 | 2017-09-19 | 宝瑞源生物技术(北京)有限公司 | A kind of human papilloma virus（HPV）Parting quick determination method |
| CN107177684A (en) * | 2017-06-15 | 2017-09-19 | 刘琳 | A kind of constant temperature nucleic acid amplification reaction reagent |
| CN109628646A (en) * | 2019-01-24 | 2019-04-16 | 郑筱玮 | A kind of detection method of the E6 or E7 nucleic acid of HPV16 or HPV18 virus based on RPA |
| CN109943663A (en) * | 2019-03-22 | 2019-06-28 | 安徽深蓝医疗科技股份有限公司 | For recombinase amplification detection method, test strips and its application of 18 genotype of HPV 16 and HPV |
Non-Patent Citations (3)
| Title |
|---|
| BIAO MA: "Isothermal Method of a Recombinase Polymerase Amplification Assay for the Detection of Most Common High-Risk Human Papillomavirus Type 16 and Type 18 DNA", 《COMPARATIVE STUDY》 * |
| JINRONG WANG: "Internally controlled recombinase-aided amplification (IC-RAA) assays for the detection of human papillomavirus genotypes 16 and 18 using extracted DNA and samples treated with nucleic acid releasing agent", 《ARCHIVES OF VIROLOGY》 * |
| 王文文: "基于EuNPs的荧光侧流免疫层析联合双重RPA检测HPV16和HPV18的价值", 《浙江临床医学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113281510A (en) * | 2021-07-23 | 2021-08-20 | 深圳市中科先见医疗科技有限公司 | Antigen colloidal gold kit for rapid virus diagnosis |
| CN117074668A (en) * | 2022-10-31 | 2023-11-17 | 安徽艾赛尔智能科技有限公司 | HPV virus typing detection kit and preparation method thereof |
| CN117757991A (en) * | 2023-12-27 | 2024-03-26 | 海宁奥羚医学检验实验室有限公司 | POCT detection method for DNA and mRNA of HPV16/18 virus gene in cervical exfoliated cells |
| CN117757991B (en) * | 2023-12-27 | 2024-05-28 | 海宁奥羚医学检验实验室有限公司 | POCT detection method for DNA and mRNA of HPV16/18 virus gene in cervical exfoliated cells |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108411036B (en) | Nucleic acid detection kit and method for rapidly detecting influenza A and influenza B viruses | |
| CN102994651B (en) | Primer, probe and kit for fluorescence PCR (Polymerase Chain Reaction) detection of 18 high-risk human papilloma viruses | |
| CN111073997A (en) | Kit for early and rapid screening of HPV16-E6 and HPV18-E6 | |
| CN101487063B (en) | Human papilloma virus infection gene amplification fluorescent detection kit | |
| Persson et al. | High-risk human papillomavirus E6/E7 mRNA and L1 DNA as markers of residual/recurrent cervical intraepithelial neoplasia | |
| CN110628953B (en) | Kit for human papilloma virus typing detection | |
| CN108330215A (en) | A kind of primer and probe compositions detecting high-risk 16 types of HPV using RPA technologies | |
| CN104017907B (en) | A kind of HPV high-risk-type fluorescence PCR detection reagent kit | |
| CN107475389B (en) | Primer group, kit and method for detecting mycoplasma pneumoniae | |
| CN104017906B (en) | A kind of HPV high-risk-type parting fluorescence PCR detection kit | |
| WO2024152423A1 (en) | Portable device for collecting menstrual blood sample and hpv rapid detection system | |
| CN104561375A (en) | Isothermal amplification detection kit and detection method of new bunyavirus | |
| CN101619364A (en) | Method for detecting HPV and application of Phi29DNA polymerase in detecting HPV | |
| CN116042370A (en) | Novel coronavirus detection kit for home self-detection and detection method thereof | |
| CN105936941A (en) | Mycobacterium tuberculosis PCR-LFB detection kit | |
| CN109402298A (en) | A kind of kit for HPV infection in qualitative detection people's cervical exfoliated cell | |
| CN115747379A (en) | Real-time fluorescent nucleic acid isothermal amplification detection kit for herpes simplex virus types 1 and 2 and special primer and probe thereof | |
| CN111850129B (en) | Primer pair, kit and method for detecting stability of NR21 locus of microsatellite | |
| CN104745722A (en) | Primers, probe and kit used for detecting varicella zoster viruses (VZVs) | |
| CN110760615B (en) | II type herpes simplex virus detection kit and detection method | |
| CN113667775A (en) | DNA probe and test strip for HCV virus nucleic acid detection and application thereof | |
| US9487820B2 (en) | Methods of nucleic acid liquid-phase extraction and detection | |
| CN111560444A (en) | Bordetella pertussis nucleic acid detection kit | |
| CN111206117A (en) | Kit for detecting human immunodeficiency virus | |
| CN107227343A (en) | It is a kind of to detect the kit and its primer pair and probe for including incubation period tuberculosis infection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200428 |
|
| WD01 | Invention patent application deemed withdrawn after publication |