CN111066826A - Medicament for preventing and treating walnut rot and application thereof - Google Patents
Medicament for preventing and treating walnut rot and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G7/00—Botany in general
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- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
- A01N61/02—Mineral oils; Tar oils; Tar; Distillates, extracts or conversion products thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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Abstract
The invention discloses a medicament for preventing and treating walnut rot and application thereof. The prevention and treatment agent is prepared by mixing 35-45 parts of wood tar, 15-30 parts of pyroligneous, 8-14 parts of sophora alopecuroides total alkaloids, 2-8 parts of isoflavone lignans, 4-10 parts of astragalin and 10-20 parts of surfactant, and is combined with a physical method of sawing diseased branches, scraping diseased spots and spraying diluent to a walnut garden, so that the use amount of pesticide can be greatly reduced, no chemical pesticide component is added in the prevention and treatment agent, the prevention and treatment agent is pollution-free and low in cost, the wound surface of physical prevention and treatment is small, the agent can penetrate into a xylem, internal and external germs can be fully contacted with the agent, the exertion of pesticide effect is facilitated, the prevention and treatment effect on walnut rot is remarkable, and the prevention and treatment agent has popularization and application values.
Description
Technical Field
The invention relates to the field of disease and insect pest control of fruit trees, in particular to the technical field of preparation of a medicament for controlling walnut rot and application thereof.
Background
The walnut (Juglas regia L.) is an important woody oil tree species, has higher economic, ecological and social benefits, and the market demand is gradually increased and the cultivation area of the walnut is continuously enlarged along with the vigorous support of the nation on the woody oil industry in recent years. The walnut rot disease is a branch disease caused by pathogenic fungi, pathogenic bacteria overwinter in diseased parts by mycelium or conidiospore apparatus, conidiospore is generated in the next year, the propagation by wind, rain water, insects and the like is realized, the peak period of disease occurrence is in spring and autumn, when the air humidity is high, a large number of conidiospore are generated, the infection harm is carried out for many times, the disease mainly harms the cortex of branches, the rot of phloem is usually caused by the infection of the pathogenic bacteria, the nutrition absorption of trees is hindered, the walnut rot disease is caused because the rough management of a fruit grower on a walnut garden is blind, the pesticide is excessively depended, the fertilizer and water management is improper, the existence of the wound of the branch is ignored, the wrong treatment is carried out on the wound, the rot disease becomes a retransmission disease in walnut planting, the walnut garden planted in a sheet, the number of damaged plants can reach 50% of the total plants, and can reach more than 80% of the damaged plants, has become a non-negligible obstacle in the development of the walnut industry. The disease risk is very serious in many important walnut producing areas in China.
At present, the common methods for treating walnut rot disease include chemical prevention and control, physical prevention and control and biological prevention and control. The chemical pesticide prevention and treatment is a method with higher prevention and treatment utilization rate of walnut rot, common fungicides are usually used and matched with corresponding antibiotics for comprehensive prevention and treatment, and common methods comprise octopamine, agricultural streptomycin, thiophanate methyl, high-concentration caustic soda and the like, but the chemical pesticide remains trace pesticide protosome and toxic metabolites in organisms, agricultural and sideline products and the environment after being used, so that the environment is polluted; the physical method is mainly used for preventing and treating by pruning and scraping disease spots, but the mode of completely scraping the disease spots can damage the phloem and xylem of a trunk, influence the nutrient transportation of the trunk, possibly further reduce the tree vigor and cause secondary infection of decay germs, and the mode is suitable for walnut trees with small disease spots, small tree ages and vigorous early bearing stage, and is not suitable for walnut trees in full bearing stage and old and weak trees; the antagonism between microorganisms for biological control realizes the comprehensive control of pathogenic bacteria, but the cost is higher. According to the current treatment method for walnut rot, a prevention and treatment means which is environment-friendly, free of chemical pesticide components, pollution-free, obvious in prevention and treatment effect on walnut rot, low in cost and wide in application range is urgently needed.
Disclosure of Invention
Aiming at the technical current situation that the physical prevention and control medicament is used in a combined way in the treatment process of the walnut rot disease in the prior art, the invention aims to provide a medicament for preventing and controlling the walnut rot disease and application thereof. The method combines the provided prevention and treatment medicament with physical prevention and treatment, can greatly reduce the use amount of pesticide, does not add chemical pesticide components in the prevention and treatment medicament, is pollution-free and low in cost, has small wound surface during physical prevention and treatment, can ensure that internal and external germs are fully contacted with the medicament, is favorable for exerting the pesticide effect, can exert the advantages of the tree body to the maximum extent, promotes the formation of callus, finally achieves the double purposes of removing diseases and stabilizing yield, has obvious prevention and treatment effect on walnut canker, and has popularization and application values.
The invention adopts the main technical scheme that:
the invention provides a medicament for preventing and treating walnut rot, which comprises 35-45 parts of wood tar, 15-30 parts of pyroligneous, 8-14 parts of total alkaloids of sophora alopecuroides, 2-8 parts of isoflavone lignan, 4-10 parts of astragalin and 10-20 parts of surfactant in parts by weight.
Preferably, the walnut rot disease prevention and treatment agent comprises, by weight, 40 parts of wood tar, 25 parts of pyroligneous, 12 parts of total alkaloids of sophora alopecuroides, 6 parts of isoflavone lignans, 6 parts of astragalin and 15 parts of a surfactant.
Meanwhile, the invention specifically provides an application of the medicament for preventing and treating walnut rot, and the specific application method comprises the following technical steps:
(1) carrying out prevention treatment after walnut beginning to sprout in spring and walnut is harvested in autumn every year, sawing off diseased branches of walnut rot, scraping off tree body rot spots to expose xylem, coating prevention and treatment agents at diseased positions, and coating the disease prevention and treatment agents for preparing walnut rot disease prevention and treatment agents provided above with a straw curtain to wrap the coated positions;
(2) spraying diluted pesticide for preventing and treating walnut rot once every 3 days for 2-4 times.
According to the invention, the branches with the walnut rot disease are sawed off, the tree body rot disease spots are scraped, and the used tools are used for treating the affected part of the walnut rot disease each time, and the affected part is soaked in the prevention and treatment agent for reuse.
According to the invention, the tree body rotting scabs are scraped, the scabs on the trunk and phloem tissues at the position of 2-3cm of radius around the scabs are scraped together, and the xylem is exposed.
The diluted prevention and treatment medicament is a solution which is diluted by 40-60 times by adding water into the prevention and treatment medicament.
Preferably, in the invention, the diluted control agent is a 50-fold solution diluted by adding water into the control agent.
Preferably, the diluted control agent is sprayed to the walnut orchard with the rot disease once every 3 days for 3 times.
The invention is characterized in that tests prove that the six medicaments and corresponding preparation proportions are adopted for compounding to prepare the control medicament, although the six medicaments are commonly known medicaments by the public, the prior art does not provide the method for compounding the six medicaments for preparing the medicament for controlling the walnut rot disease to prepare the control medicament for controlling the walnut rot disease, and the control medicament for controlling the walnut rot disease is prepared without adding chemical pesticide components, has no pollution, has obvious control effect on the walnut rot disease and has low cost; the walnut rot disease prevention and control agent is combined with the prevention and control method provided by the application, so that the wound of a walnut tree is small, the agent can penetrate into xylem, internal and external germs can be fully contacted with the agent, the exertion of drug effect is facilitated, the whole walnut garden can be effectively prevented and controlled, the propagation and transfer of the pathogens are effectively prevented and controlled, meanwhile, the advantages of the tree body can be exerted to the greatest extent, the formation of callus is promoted, the dual purposes of disease removal and yield stabilization are finally achieved, and the problems that the walnut rot disease is not environment-friendly due to the use of chemical pesticides, the physical treatment is not thorough, and the walnut yield is influenced in the current treatment mode of the walnut rot disease are solved.
By implementing the specific inventive content of the present invention, the following specific beneficial effects can be achieved:
(1) the medicament for preventing and treating the walnut rot disease is prepared by compounding wood tar, pyroligneous, total alkaloids of sophora alopecuroides, isoflavone lignans, astragalin, a surfactant and the like in proportion, is free from adding chemical pesticide components, has no pollution, has obvious effect of preventing and treating the walnut rot disease, and is low in cost.
(2) The invention provides an application for preparing a medicament for preventing and treating walnut rot, which can greatly reduce the using amount of pesticides by combining the physical methods of sawing diseased branches, scraping diseased spots and spraying diluent to a walnut garden, has small wound surface for physical prevention and treatment, can ensure that the pesticides in the interior and the exterior can be fully contacted with the pesticides, is beneficial to exerting the pesticide effect, can effectively prevent and treat the whole walnut garden, effectively prevents and blocks the propagation and transfer of pathogenic bacteria, can exert the advantages of a tree body to the greatest extent, promotes the formation of callus, and finally achieves the dual purposes of removing diseases and stabilizing the yield, the recurrence rate of the diseased spots after the prevention and treatment by the prevention and treatment method is only 4 percent, the prevention and treatment effect is 96 percent, the yield of a single plant is 14.6kg, and the prevention and treatment effect on the walnut rot is obvious, has popularization and application value.
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Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples.
All raw and auxiliary materials selected in the invention and selected preparation methods are well known and selected in the art, and all percentages referred to in the invention are percentages by weight unless otherwise indicated.
The first embodiment is as follows: used for preparing a medicament for preventing and treating walnut rot
The invention provides a medicament for preventing and treating walnut rot, which comprises 35-45 parts of wood tar, 15-30 parts of pyroligneous, 8-14 parts of total alkaloids of sophora alopecuroides, 2-8 parts of isoflavone lignan, 4-10 parts of astragalin and 10-20 parts of surfactant in parts by weight.
Example two: application of preparation for preventing and treating walnut rot
The invention particularly provides application of a medicament for preventing and treating walnut rot, which comprises the following steps:
(1) carrying out prevention treatment after walnut beginning to sprout in spring and walnut is harvested in autumn every year, sawing off diseased branches of walnut rot, scraping off tree body rot spots to expose xylem, coating prevention and treatment agents at diseased positions, and coating the disease prevention and treatment agents for preparing walnut rot disease prevention and treatment agents provided above with a straw curtain to wrap the coated positions;
(2) the diluted prevention and treatment agent is sprayed to the walnut orchard suffering from the rot once every 3 days for 2-4 times.
According to the invention, the branches with the walnut rot disease are sawed off, the tree body rot disease spots are scraped, and the used tools are used for treating the affected part of the walnut rot disease each time, and the affected part is soaked in the prevention and treatment agent for reuse.
According to the invention, the tree body rotting scabs are scraped, the scabs on the trunk and phloem tissues at the position of 2-3cm of radius around the scabs are scraped together, and the xylem is exposed.
The diluted prevention and treatment medicament is a solution which is diluted by 40-60 times by adding water into the prevention and treatment medicament.
Example three: used for preparing a medicament for preventing and treating walnut rot
Carrying out prevention treatment after walnut beginning to sprout in spring and walnut is harvested in autumn every year, sawing off diseased branches of walnut rot, scraping disease spots on a trunk together with phloem tissues at the position of 2-3cm of radius around the disease spots as the center to expose xylem, smearing prevention and treatment agents at diseased positions, wrapping the prevention and treatment agents with a straw curtain, and wrapping the smearing positions; spraying 40 times of control agent diluent in a walnut garden, and spraying for 2 times every 3 days; the pesticide for preventing and treating walnut rot disease comprises 3.5kg of wood tar, 1.5kg of pyroligneous, 1.4kg of total alkaloids of sophora alopecuroides, 0.8kg of isoflavone lignans, 0.8kg of astragalus membranaceus and 1.5kg of surfactant.
Example four: used for preparing a medicament for preventing and treating walnut rot
Carrying out prevention treatment after walnut beginning to sprout in spring and walnut is harvested in autumn every year, sawing off diseased branches of walnut rot, scraping disease spots on a trunk together with phloem tissues at the position of 2-3cm of radius around the disease spots as the center to expose xylem, smearing prevention and treatment agents at diseased positions, wrapping the prevention and treatment agents with a straw curtain, and wrapping the smearing positions; spraying 50 times of control agent diluent in a walnut orchard once every 3 days for 3 times; the pesticide for preventing and treating walnut rot disease comprises 4kg of wood tar, 2.5kg of pyroligneous, 1.2kg of total alkaloids of sophora alopecuroides, 0.6kg of isoflavone lignan, 0.6kg of astragalus membranaceus and 1.5kg of surfactant.
Example five: used for preparing a medicament for preventing and treating walnut rot
Carrying out prevention treatment after walnut beginning to sprout in spring and walnut is harvested in autumn every year, sawing off diseased branches of walnut rot, scraping disease spots on a trunk together with phloem tissues at the position of 2-3cm of radius around the disease spots as the center to expose xylem, smearing prevention and treatment agents at diseased positions, wrapping the prevention and treatment agents with a straw curtain, and wrapping the smearing positions; spraying 60 times of control agent diluent in a walnut orchard once every 3 days for 4 times; the pesticide for preventing and treating walnut rot disease comprises 4.5kg of wood tar, 2kg of pyroligneous, 0.8kg of total alkaloids of sophora alopecuroides, 0.2kg of isoflavone lignans, 1kg of astragalus membranaceus and 1kg of surfactant.
Example six: used for preparing a medicament for preventing and treating walnut rot
Carrying out prevention treatment after walnut beginning to sprout in spring and walnut is harvested in autumn every year, sawing off diseased branches of walnut rot, scraping disease spots on a trunk together with phloem tissues at the position of 2-3cm of radius around the disease spots as the center to expose xylem, smearing prevention and treatment agents at diseased positions, wrapping the prevention and treatment agents with a straw curtain, and wrapping the smearing positions; spraying 40 times of control agent diluent in a walnut garden, and spraying for 2 times every 3 days; the pesticide for preventing and treating walnut rot disease comprises 4kg of wood tar, 3kg of pyroligneous, 1kg of total alkaloids of sophora alopecuroides, 0.4kg of isoflavone lignan, 0.4kg of astragalus membranaceus and 2kg of surfactant.
Example seven: used for preparing a medicament for preventing and treating walnut rot
Carrying out prevention treatment after walnut beginning to sprout in spring and walnut is harvested in autumn every year, sawing off diseased branches of walnut rot, scraping disease spots on a trunk together with phloem tissues at the position of 2-3cm of radius around the disease spots as the center to expose xylem, smearing prevention and treatment agents at diseased positions, wrapping the prevention and treatment agents with a straw curtain, and wrapping the smearing positions; spraying 40 times of control agent diluent in a walnut garden, and spraying 3 times in total once every 3 days; the pesticide for preventing and treating walnut rot disease comprises 4kg of wood tar, 3kg of pyroligneous, 0.8kg of total alkaloids of sophora alopecuroides, 0.2kg of isoflavone lignan, 1kg of astragalus membranaceus and 1kg of surfactant.
Example eight: optimization test for preparing medicament for preventing and treating walnut rot
Mixing wood tar, pyroligneous, total alkaloids of sophora alopecuroides, isoflavone lignans, astragalin and surfactant according to different proportions, wherein the proportions are 9 combinations, and the different proportions are shown in table 1, obtaining the control medicament for the walnut rot disease, treating the scab in 3 months, sawing off the diseased branches of the walnut rot disease, scraping the rot disease scab of the tree body, exposing the xylem, smearing the control medicament on the diseased part, wrapping the control medicament with a straw mat, and wrapping the smeared part; spraying 50 times of the diluent of the prevention and treatment agent in a walnut garden, and comparing the treatment effects of different prevention and treatment agent combinations by investigating the disease spot recurrence rate in 10 months.
Determination of the recurrence rate of lesions: randomly selecting 25 samples in the walnut garden, repeating the data for 3 times, investigating the recurrence condition of the disease spots, and calculating the recurrence rate of the disease spots, wherein the results are shown in table 1.
Table 1: influence of different combinations of prevention and treatment agents on recurrence rate of disease spots
In the optimization test of the formula of the walnut rot disease prevention and treatment agent, the results are shown in table 1, and the walnut rot disease prevention and treatment agent is prepared by mixing wood tar, wood vinegar, sophora alopecuroide total alkaloids, isoflavone lignans, astragalin and a surfactant according to different proportions, wherein the number of recurrent disease spots of the walnut rot disease prevention and treatment agent in the group E proportion is 1, the recurrence rate of the disease spots is 4%, and the recurrence rate is the lowest, so the optimal combination is selected: 40 parts of wood tar, 25 parts of pyroligneous, 12 parts of total alkaloids of sophora alopecuroides, 6 parts of isoflavone lignans, 6 parts of astragalus membranaceus and 15 parts of surfactant.
Example nine: application optimization test for preparing medicament for preventing and treating walnut rot
The test respectively explores the influence of scraping the range of rotten disease spots, preventing and treating the dilution double liquid of the medicament and spraying times on the recurrence rate of the rotten disease spots of the walnuts. A three-factor three-level orthogonal test is carried out on the basis of the single-factor test, and the factors and the levels of the orthogonal test are shown in a table 2.
Table 2: orthogonal test factor and horizon
The incidence and analysis of walnut rot disease spots under different control conditions by orthogonal optimization are shown in Table 3.
Table 3: results and analysis of orthogonal assays
As can be seen from Table 3, the dilution factor of the control agent has the greatest effect on the walnut rot disease within the range of experimental design. The best optimization condition obtained by combining the table 2 and the table 3 is A2B2C2Namely, the range of the rotten disease spots is scraped by 2-3cm, the control agent is diluted by 50 times, and the disease spots have the lowest recurrence rate when the control agent is sprayed for 3 times.
Obtained by experimental optimization of the above series of examples: the medicament for preventing and treating walnut rot is prepared by mixing 40 parts of wood tar, 25 parts of pyroligneous, 12 parts of total alkaloids of sophora alopecuroides, 6 parts of isoflavone lignan, 6 parts of astragalin and 15 parts of surfactant; the disease-causing branches of the walnut rot disease are sawed off, disease spots on a trunk and phloem tissues at the positions with the disease spots as the centers and the radiuses of 2-3cm are scraped together to expose xylem, the disease positions are coated with the agent for preventing and treating the walnut rot disease, and grass curtains are wrapped and attached to prepare the agent for preventing and treating the walnut rot disease to wrap the coated positions; spraying 50 times of control agent diluent in a walnut orchard once every 3 days for 3 times.
Example ten: an application optimization test for preparing the medicament for preventing and treating the walnut rot disease is tested in a certain walnut planting base in West county in Xinjiang, and the test results of the five test groups are compared by setting 5 test groups, wherein the group A is a blank control group, the group B is the control method adopting the application, the group C is a single-use physical control method, the group D is a single-use chemical agent control method, the group E is a scheme II method in 'evaluation of different control methods for the walnut rot disease in Liaoxi area' which adopts the Zenghui in the prior art.
The prevention and treatment effect calculation formula is as follows:
the preventing and treating effect (%) is (CK lesion recurrence rate-lesion recurrence rate)/CK lesion recurrence rate 100%
Table 4: influence of different control methods on control of walnut rot
By comparing the four indexes of the number of recurrent lesions, the recurrence rate of the lesions, the prevention and treatment effect and the single plant yield, the results are shown in Table 4, as can be seen from different prevention and treatment methods, the disease spot recurrence rate of the B group prevention and treatment method is only 4 percent, the prevention and treatment effect is 96 percent, the single plant yield is 14.6kg, compared with the group C, the group D and the group E, the prevention and treatment effect and the single plant yield are greatly improved, the number of recurrent disease spots and the recurrence rate of the disease spots are lowest, the prevention and treatment effect is optimal, and the physical prevention and treatment of the wound surface is small, the medicament can penetrate into the xylem, so that internal and external pathogens are fully contacted with the medicament, the exertion of the medicament effect is facilitated, meanwhile, the method can also exert the advantages of the tree body to the maximum extent, promote the formation of callus, finally achieve the dual purposes of removing diseases and stabilizing yield, has obvious effect of preventing and treating walnut canker, and has popularization and application values.
The above examples are merely illustrative for clearly illustrating the present invention and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made while remaining within the scope of the present invention.
Claims (9)
1. The medicament for preventing and treating walnut rot is characterized by comprising, by weight, 35-45 parts of wood tar, 15-30 parts of pyroligneous, 8-14 parts of total alkaloids of sophora alopecuroides, 2-8 parts of isoflavone lignans, 4-10 parts of astragalus membranaceus and 10-20 parts of a surfactant.
2. The preparation of the medicament for preventing and treating walnut rot as claimed in claim 1, wherein the medicament comprises 40 parts of wood tar, 25 parts of pyroligneous, 12 parts of total alkaloids of sophora alopecuroides, 6 parts of isoflavone lignans, 6 parts of astragalin and 15 parts of surfactant in parts by weight.
3. Use of the agent for preventing and treating walnut rot as claimed in claim 1 in walnut planting.
4. An application of a medicament for preventing and treating walnut rot disease comprises the following technical steps:
(1) the walnut trees are treated by preventing and treating after the walnut trees begin to sprout in spring and are harvested in autumn every year, diseased branches of walnut rot disease are sawn off, tree body rot disease spots are scraped, xylem is exposed, a preventing and treating agent is coated on diseased positions, the provided agent for preventing and treating walnut rot disease is coated by a straw curtain, and the coated positions are coated;
(2) spraying diluted pesticide for preventing and treating walnut rot once every 3 days for 2-4 times.
5. The use of the agent for the preparation of a preventive agent against walnut decay disease according to claim 4, wherein the branches of walnut decay disease are sawn off, the rotten spots of the tree are scraped off, and the used tools are used for treating the rotten spots of walnut each time, and the rotten spots of walnut are soaked in the agent for the prevention and the treatment provided in claim 1 and then reused.
6. The use of the medicament for preparing a medicament for preventing and treating walnut rot as claimed in claim 4, wherein the disease spots of the tree body rot are scraped, and the disease spots on the trunk are scraped together with the phloem tissue at the 2-3cm radius around the disease spots as the center, so that the xylem is exposed.
7. The use of claim 4 for preparing a medicament for the prevention and treatment of walnut decay disease, wherein the diluted medicament is diluted by 40-60 times with water.
8. The use of claim 4 for preparing a medicament for the prevention and treatment of walnut rot, wherein the diluted medicament is diluted by 50 times with water.
9. The use of the agent for preventing and treating walnut rot as claimed in claim 4, wherein the diluted agent is sprayed once every 3 days for 3 times.
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