CN110927376B - 一种喹乙醇的磁免疫化学发光检测试剂盒及其应用 - Google Patents
一种喹乙醇的磁免疫化学发光检测试剂盒及其应用 Download PDFInfo
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- CN110927376B CN110927376B CN201910948224.8A CN201910948224A CN110927376B CN 110927376 B CN110927376 B CN 110927376B CN 201910948224 A CN201910948224 A CN 201910948224A CN 110927376 B CN110927376 B CN 110927376B
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- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G01N33/532—Production of labelled immunochemicals
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
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- Pathology (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
免疫抗原 | 检测抗原 | 抗血清效价 |
OLA-A-BSA实施例1 | OLA-A-OVA实施例1 | 512000 |
OLA-HS-BSA对比例1 | OLA-HS-OVA对比例1 | 102400 |
Claims (1)
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CN201910948224.8A CN110927376B (zh) | 2019-10-08 | 2019-10-08 | 一种喹乙醇的磁免疫化学发光检测试剂盒及其应用 |
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CN201910948224.8A CN110927376B (zh) | 2019-10-08 | 2019-10-08 | 一种喹乙醇的磁免疫化学发光检测试剂盒及其应用 |
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CN110927376A CN110927376A (zh) | 2020-03-27 |
CN110927376B true CN110927376B (zh) | 2024-04-30 |
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CN1888903A (zh) * | 2006-08-08 | 2007-01-03 | 中国农业科学院农业质量标准与检测技术研究所 | 一种检测喹乙醇的酶联免疫试剂盒 |
CN102621322A (zh) * | 2012-03-29 | 2012-08-01 | 北京维德维康生物技术有限公司 | 检测3-甲基喹噁啉-2-羧酸的试剂盒 |
CN103146652A (zh) * | 2013-02-19 | 2013-06-12 | 中国农业科学院兰州畜牧与兽药研究所 | 抗喹乙醇单克隆抗体及其杂交瘤细胞株、其制备方法及用于检测饲料中喹乙醇的试剂盒 |
CN105061339A (zh) * | 2015-07-02 | 2015-11-18 | 中国兽医药品监察所 | 一种半抗原、其人工抗原及其在检测喹乙醇残留标志物中的应用 |
CN107677807A (zh) * | 2016-09-30 | 2018-02-09 | 青岛大学 | 一种吉他霉素磁免疫化学发光检测试剂盒 |
-
2019
- 2019-10-08 CN CN201910948224.8A patent/CN110927376B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1888903A (zh) * | 2006-08-08 | 2007-01-03 | 中国农业科学院农业质量标准与检测技术研究所 | 一种检测喹乙醇的酶联免疫试剂盒 |
CN102621322A (zh) * | 2012-03-29 | 2012-08-01 | 北京维德维康生物技术有限公司 | 检测3-甲基喹噁啉-2-羧酸的试剂盒 |
CN103146652A (zh) * | 2013-02-19 | 2013-06-12 | 中国农业科学院兰州畜牧与兽药研究所 | 抗喹乙醇单克隆抗体及其杂交瘤细胞株、其制备方法及用于检测饲料中喹乙醇的试剂盒 |
CN105061339A (zh) * | 2015-07-02 | 2015-11-18 | 中国兽医药品监察所 | 一种半抗原、其人工抗原及其在检测喹乙醇残留标志物中的应用 |
CN107677807A (zh) * | 2016-09-30 | 2018-02-09 | 青岛大学 | 一种吉他霉素磁免疫化学发光检测试剂盒 |
Non-Patent Citations (1)
Title |
---|
喹乙醇mAb的制备及其dcTRFIA分析方法的建立;桑永玉;《全国优秀硕士学位论文全文数据库》;20150515;第1-68页 * |
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