CN110786438A - Segmented extraction method of moringa oleifera leaf effective components, and beverage preparation method containing moringa oleifera leaf effective components - Google Patents
Segmented extraction method of moringa oleifera leaf effective components, and beverage preparation method containing moringa oleifera leaf effective components Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the field of moringa oleifera extract and a production method thereof, in particular to a segmented extraction method of active ingredients of moringa oleifera leaves and a beverage containing the active ingredients of the moringa oleifera leaves and a preparation method of the beverage, comprising the following steps: feeding the raw material moringa leaves into a heat pump dryer for heat pump drying; crushing the dried moringa leaves into powder, and drying by hot air; mixing the powder and deionized water, stirring, homogenizing, continuously extracting the mixed solution with two tanks, finely filtering and ultrafiltering the water extract, spray drying to obtain water extract powder, centrifuging to obtain solid, mixing with ethanol, and ultrasonically extracting to obtain ethanol extract; evaporating ethanol in the ethanol leaching solution under low pressure, and drying to obtain ethanol extract powder. The method for extracting the active ingredients of the moringa leaves by sections can extract various effective nutrient substances, has high extraction rate, little damage to heat-sensitive and light-sensitive nutrient substances in the moringa leaves and high extraction rate of protein.
Description
Technical Field
The invention relates to the field of moringa oleifera extracts and production methods thereof, and particularly relates to a segmented extraction method of active ingredients of moringa oleifera leaves and a preparation method of drinks and drinks containing the active ingredients of the moringa oleifera leaves.
Background
The moringa oleifera as vegetable and food has the functions of improving nutrition and dietotherapy health care, and meanwhile, the moringa oleifera is widely applied to the aspects of medicine, health care and the like and is known as a living tree. The moringa oleifera contains rich nutrients, is widely applied to improving various diseases, has obvious effect, and is regarded as a saving star of modern medicine by scientists. All parts of moringa oleifera, such as leaves, roots, seeds, bark, fruits, flowers and immature pods, can be used as a medicine. The moringa leaves have good adjuvant treatment effect on hypertension, hyperlipidemia, diabetes and the like. Has effects in relaxing bowels, removing toxic substances, strengthening body constitution, caring skin; increasing the calcium content of bone, enhancing the ability of reluctant excitation, preventing diseases, improving sleep, enhancing memory and delaying aging; the moringa leaf powder contains various amino acids, mineral substances and vitamins, can repair tissue and organism injuries and correct cell functions, and is very beneficial to the growth of children. The moringa aqueous extract contains rich proteins, amino acids and other nutritional ingredients, can supplement proteins and amino acids required by a human body, can reduce the increase of blood sugar caused by streptomicin, reduce the percentage of hemoglobin HBA1C and reduce the lipid peroxidation level, and flavonoids and polyphenol in the ethanol extract in the moringa leaves have the effect of reducing the blood sugar and have high antioxidant activity.
The traditional moringa production and processing is also in simple rough processing, the moringa leaves are usually dried and crushed into powder, and the effective components in the moringa leaves are not effectively extracted and are difficult to be effectively absorbed by a human body; the existing fine processing extraction process has the following defects: only single nutrient substances (such as protein, polysaccharide, fat and the like) can be extracted, the synergistic effect of various nutrient substances cannot be achieved, and the nutrient substances are not extracted completely, so that waste is caused; the existing drying process usually adopts treatment methods such as hot air drying, sun drying or microblog drying, and obviously damages heat-sensitive and photosensitive nutrient substances in moringa leaves; the existing protein extraction mostly adopts a microwave extraction or composite protease extraction process, the extraction rate of microwave extraction is low, usually about thirty percent, while the extraction rate of the composite protease extraction process is slightly high, but the extraction efficiency is still not very high because the cellulose of cell walls is not decomposed and destroyed.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a segmented extraction method of the active ingredients of the moringa leaves, a beverage containing the active ingredients of the moringa leaves and a preparation method of the beverage, wherein the beverage can be used for extracting various effective nutrient substances, has high extraction rate, little damage to heat-sensitive and light-sensitive nutrient substances in the moringa leaves and high extraction rate of protein, and has various effects.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a segmented extraction method for active ingredients of moringa leaves is characterized by comprising the following steps:
step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 30-35 ℃, and the drying time is 3-4 hours;
step 2, crushing the dried moringa leaves obtained in the step 1 into powder;
step 3, carrying out hot air drying on the powder obtained in the step 2, wherein the drying temperature is 40-45 ℃, and the drying time is 1-2 hours;
and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: mixing and stirring at the weight ratio of 100-120 and homogenizing;
and 5, regulating the pH value of the homogenized mixed solution to 4-4.5, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the ratio of cellulase to the mixed solution 1: adding cellulase in a weight ratio of 100-: adding 100-110 weight percent of composite protease, centrifuging the mixed solution output by the second extraction tank to separate the solid and the liquid to obtain water extract;
step 6, carrying out fine filtration and ultrafiltration on the water extract, and carrying out spray drying to form water extract powder;
and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 20-30, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching solution;
and 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.
Preferably, the powder in the step 3 is dried by hot air and then stored in a thermostat at 25 ℃.
Preferably, the stirring in step 4 is carried out in a constant temperature magnetic stirrer, the stirring time is 1-1.5 hours, and the mixture is homogenized at 300kg/cm2 in a homogenizer.
Preferably, in the step 5, the set temperature of the first extraction tank is 45 ℃, the extraction time is 35min, the stirring speed during extraction is 70r/min, the enzyme is immediately deactivated by using a boiling water bath after the time is up, the set temperature of the second extraction tank is 50 ℃, the extraction time is 35min, the stirring is not performed during extraction, and the enzyme is immediately deactivated by using a boiling water bath after the time is up for 15 min.
Preferably, the ultrafiltration in step 6 is ceramic membrane ultrafiltration.
Preferably, the power of the ultrasonic extraction in the step 7 is 300W, the extraction temperature is 50 ℃, and the extraction time is 45 min.
A beverage containing the active ingredients of the moringa leaves is characterized in that water extract powder and ethanol extract powder extracted by the segmented extraction method of the active ingredients of the moringa leaves of any one of claims 1 to 6 are used for preparation, and the beverage comprises the following components in parts by weight:
10-20 parts of water extract powder, 5-10 parts of ethanol extract powder, 10-20 parts of medlar powder, 5-7 parts of sweet osmanthus powder, 8-10 parts of honeysuckle powder and 3-5 parts of liquorice powder.
Preferably, the composition comprises the following components in parts by weight: 15 parts of water extract powder, 7 parts of ethanol extract powder, 15 parts of medlar powder, 6 parts of sweet osmanthus powder, 9 parts of honeysuckle powder and 4 parts of liquorice powder.
A method for preparing the beverage containing the active ingredients of the moringa oleifera leaves in claim 7 or 8, wherein the method comprises the following steps: the method comprises the following steps:
firstly, drying medlar, sweet osmanthus, honeysuckle and liquorice by hot air at 45 ℃ respectively, and crushing into powder;
mixing the water extract powder, the ethanol extract powder, the medlar powder, the sweet osmanthus powder, the honeysuckle powder and the liquorice powder in proportion, and uniformly stirring;
and thirdly, packaging the mixed powder according to 10g per package.
From the above description, the segmented extraction method of the moringa oleifera leaf effective components and the preparation method of the beverage and the beverage containing the moringa oleifera leaf effective components provided by the invention have the following beneficial effects:
the water extraction method and the organic matter extraction method are adopted for extracting the nutrient substances in a segmented manner, so that various effective components such as protein, polysaccharide, amino acid, flavone, polyphenol and the like in the moringa leaves can be extracted, the nutrient substances are completely extracted, the utilization rate is high, and the drink prepared from various nutrient substances has various synergistic effects; the hot pump drying is adopted before crushing, the heat pump drying temperature is low, the damage to heat-sensitive and photosensitive nutrient substances in the moringa leaves is small, the contents of fat and polysaccharide in the moringa leaves are well kept, and the hot air drying is further adopted for a short time after crushing, so that the water content is further reduced, and the extraction rate of effective components in the subsequent steps is improved; the beverage prepared by the method has multiple composite effects, can supplement protein required by a human body, reduce blood sugar increase caused by streptomicin, and reduce lipid peroxidation level, and flavonoid substances and polyphenol in ethanol extract in moringa oleifera leaves have the effect of reducing blood sugar and have high antioxidant activity.
Detailed Description
The invention is further described below by means of specific embodiments.
A segmented extraction method of active ingredients of moringa leaves comprises the following steps:
step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 30-35 ℃, and the drying time is 3-4 hours;
step 2, crushing the dried moringa leaves obtained in the step 1 into powder;
step 3, carrying out hot air drying on the powder obtained in the step 2, wherein the drying temperature is 40-45 ℃, and the drying time is 1-2 hours;
and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: mixing and stirring at the weight ratio of 100-120 and homogenizing;
and 5, regulating the pH value of the homogenized mixed solution to 4-4.5, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the ratio of cellulase to the mixed solution 1: adding cellulase in a weight ratio of 100-: adding 100-110 weight percent of composite protease, centrifuging the mixed solution output by the second extraction tank to separate the solid and the liquid to obtain water extract;
step 6, carrying out fine filtration and ultrafiltration on the water extract, and carrying out spray drying to form water extract powder;
and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 20-30, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching solution;
and 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.
The first embodiment is as follows:
the segmented extraction method of the active ingredients of the moringa leaves comprises the following steps:
step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 32 ℃, and the drying time is 3.5 hours;
step 2, crushing the dried moringa leaves obtained in the step 1 into powder;
step 3, drying the powder obtained in the step 2 by hot air at 42 ℃ for 1.5 hours, and storing the dried powder in a thermostat at 25 ℃;
and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: 110, stirring in a constant-temperature magnetic stirrer for 1.2 hours, and homogenizing in a homogenizer at 300kg/cm 2;
and 5, regulating the pH value of the homogenized mixed solution to 4.2, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the pH value of the mixed solution is regulated according to the ratio of cellulase to the mixed solution 1: 105, adding cellulase, and mixing the compound protease and the mixed solution 1: 105, adding compound protease, centrifuging the mixed liquor output by the second extraction tank, and performing solid-liquid separation to obtain water extract, wherein the set temperature of the first extraction tank is 45 ℃, the extraction time is 35min, the stirring speed during extraction is 70r/min, the enzyme is immediately inactivated for 15min by using a boiling water bath after the time is up, the set temperature of the second extraction tank is 50 ℃, the extraction time is 35min, the stirring is not performed during extraction, and the enzyme is immediately inactivated for 15min by using the boiling water bath after the time is up.
Step 6, performing fine filtration and ultrafiltration on the water extract, wherein the fine filtration is to sequentially filter the water extract through 100-mesh and 40-mesh filter cloth, the ultrafiltration is to perform ultrafiltration through a ceramic membrane, and the filtered liquid is spray-dried to form water extract powder;
and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 25, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching liquor, wherein the ultrasonic extraction power is 300W, the extraction temperature is 50 ℃, and the extraction time is 45 min.
And 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.
The second embodiment is as follows:
the segmented extraction method of the active ingredients of the moringa leaves comprises the following steps:
step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 30 ℃, and the drying time is 3 hours;
step 2, crushing the dried moringa leaves obtained in the step 1 into powder;
step 3, drying the powder obtained in the step 2 by hot air at the drying temperature of 40 ℃ for 1 hour, and storing the dried powder in a thermostat at the temperature of 25 ℃ after the hot air drying;
and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: 100, stirring in a constant-temperature magnetic stirrer for 1 hour, and homogenizing in a homogenizer at 300kg/cm 2;
and 5, adjusting the pH value of the homogenized mixed solution to 4, and performing continuous double-irrigation extraction, wherein the ratio of cellulase to the mixed solution 1 in a first extraction tank is as follows: adding cellulase according to the weight ratio of 100, and adding the composite protease and the mixed solution 1: adding compound protease at a weight ratio of 100, centrifuging the mixed solution output by the second extraction tank, and carrying out solid-liquid separation to obtain water extract, wherein the temperature of the first extraction tank is set to be 45 ℃, the extraction time is 35min, the stirring speed during extraction is 70r/min, the enzyme is immediately inactivated for 15min by using a boiling water bath after the time is up, the temperature of the second extraction tank is set to be 50 ℃, the extraction time is 35min, the stirring is not carried out during extraction, and the enzyme is immediately inactivated for 15min by using the boiling water bath after the time is up.
Step 6, performing fine filtration and ultrafiltration on the water extract, wherein the fine filtration is to sequentially filter the water extract through 100-mesh and 40-mesh filter cloth, the ultrafiltration is to perform ultrafiltration through a ceramic membrane, and the filtered liquid is spray-dried to form water extract powder;
and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 20, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching liquor, wherein the ultrasonic extraction power is 300W, the extraction temperature is 50 ℃, and the extraction time is 45 min.
And 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.
The third concrete embodiment:
the segmented extraction method of the active ingredients of the moringa leaves comprises the following steps:
step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 35 ℃, and the drying time is 4 hours;
step 2, crushing the dried moringa leaves obtained in the step 1 into powder;
step 3, drying the powder obtained in the step 2 by hot air at the drying temperature of 45 ℃ for 2 hours, and storing the dried powder in a thermostat at the temperature of 25 ℃ after the hot air is dried;
and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: 120, stirring in a constant-temperature magnetic stirrer for 1.5 hours, and homogenizing in a homogenizer at 300kg/cm 2;
and 5, regulating the pH value of the homogenized mixed solution to 4.5, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the pH value of the mixed solution is regulated according to the ratio of cellulase to the mixed solution 1: 110, adding cellulase, and extracting in a second extraction tank according to the weight ratio of the compound protease to the mixed solution 1: 110, centrifuging the mixed solution output by the second extraction tank to separate solid and liquid to obtain water extract, wherein the first extraction tank is set at 45 ℃, the extraction time is 35min, the stirring speed is 70r/min during extraction, the enzyme is immediately inactivated for 15min by using a boiling water bath after the time is reached, the second extraction tank is set at 50 ℃, the extraction time is 35min, the stirring is not performed during extraction, and the enzyme is immediately inactivated for 15min by using the boiling water bath after the time is reached.
Step 6, performing fine filtration and ultrafiltration on the water extract, wherein the fine filtration is to sequentially filter the water extract through 100-mesh and 40-mesh filter cloth, the ultrafiltration is to perform ultrafiltration through a ceramic membrane, and the filtered liquid is spray-dried to form water extract powder;
and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 30, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching liquor, wherein the ultrasonic extraction power is 300W, the extraction temperature is 50 ℃, and the extraction time is 45 min.
And 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.
A beverage containing the active ingredients of the moringa leaves is prepared by using water extract powder (extracted in step 6) and ethanol extract powder (extracted in step 8) extracted by implementing the sectional extraction method of the active ingredients of the moringa leaves in one to three steps, and comprises the following components in parts by weight:
10-20 parts of water extract powder, 5-10 parts of ethanol extract powder, 10-20 parts of medlar powder, 5-7 parts of sweet osmanthus powder, 8-10 parts of honeysuckle powder and 3-5 parts of liquorice powder.
Preferably, the composition comprises the following components in parts by weight: 15 parts of water extract powder, 7 parts of ethanol extract powder, 15 parts of medlar powder, 6 parts of sweet osmanthus powder, 9 parts of honeysuckle powder and 4 parts of liquorice powder.
The water extract powder contains water soluble nutrients such as protein, amino acids, and polysaccharide; the ethanol extract contains nutrients easily soluble in organic solvent, such as flavone and polyphenol.
A preparation method of a beverage containing active ingredients of moringa leaves comprises the following steps:
firstly, drying medlar, sweet osmanthus, honeysuckle and liquorice by hot air at 45 ℃, crushing into powder, and sieving by a 50-mesh sieve;
mixing the water extract powder, the ethanol extract powder, the medlar powder, the sweet osmanthus powder, the honeysuckle powder and the liquorice powder in proportion, and uniformly stirring;
and thirdly, packaging the mixed powder according to 10g per package.
The water extraction method and the organic matter extraction method are adopted for extracting the nutrient substances in a segmented manner, so that various effective components such as protein, polysaccharide, amino acid, flavone, polyphenol and the like in the moringa leaves can be extracted, the nutrient substances are completely extracted, the utilization rate is high, and the drink prepared from various nutrient substances has various synergistic effects; the hot pump drying is adopted before crushing, the heat pump drying temperature is low, the damage to heat-sensitive and photosensitive nutrient substances in the moringa leaves is small, the contents of fat and polysaccharide in the moringa leaves are well kept, and the hot air drying is further adopted for a short time after crushing, so that the water content is further reduced, and the extraction rate of effective components in the subsequent steps is improved; the method adopts double-tank extraction of cellulase and compound protease, the cellulase firstly hydrolyzes and breaks cellulose of cell walls to fully release cell sap, the compound protease is further enzymolyzed, the extraction rate of protein can reach 65-70%, compared with the extraction methods of a single compound protease extraction method (the extraction rate is about 52-55%), a single cellulase extraction method (the extraction rate is about 40-45%), a microwave extraction method (the extraction rate is about 30-35%), and the like, the extraction rate is greatly improved, the beverage prepared by the method has various compound effects, not only can supplement protein required by a human body, but also can reduce blood sugar increase caused by streptomicin and reduce lipid peroxidation level, flavonoids and polyphenol in ethanol extract in moringa oleifera leaves have the function of reducing blood sugar, and has high antioxidant activity, and the packaged product processed by the preparation method of the beverage containing the active ingredients of the moringa oleifera leaves is convenient to brew, has high nutritive value.
The above description is only a few specific embodiments of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications made by the design concept should fall within the scope of the present invention.
Claims (9)
1. A segmented extraction method for active ingredients of moringa leaves is characterized by comprising the following steps:
step 1, feeding the raw material moringa leaves into a heat pump dryer for heat pump drying, wherein the drying temperature is 30-35 ℃, and the drying time is 3-4 hours;
step 2, crushing the dried moringa leaves obtained in the step 1 into powder;
step 3, carrying out hot air drying on the powder obtained in the step 2, wherein the drying temperature is 40-45 ℃, and the drying time is 1-2 hours;
and 4, mixing the powder obtained in the step 3 and deionized water according to the weight ratio of 1: mixing and stirring at the weight ratio of 100-120 and homogenizing;
and 5, regulating the pH value of the homogenized mixed solution to 4-4.5, and carrying out continuous double-irrigation extraction, wherein in the first extraction tank, the ratio of cellulase to the mixed solution 1: adding cellulase in a weight ratio of 100-: adding 100-110 weight percent of composite protease, centrifuging the mixed solution output by the second extraction tank to separate the solid and the liquid to obtain water extract;
step 6, carrying out fine filtration and ultrafiltration on the water extract, and carrying out spray drying to form water extract powder;
and 7, carrying out centrifugal separation on the solid obtained in the step 5 and ethanol according to the weight ratio of 1: 20-30, performing ultrasonic extraction, and centrifuging to separate solid from liquid to obtain ethanol leaching solution;
and 8, evaporating the ethanol in the ethanol leaching liquor at low pressure, and drying to obtain ethanol extract powder.
2. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: and 3, drying the powder in the step 3 by hot air, and storing in a thermostat at 25 ℃.
3. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: the stirring in the step 4 is carried out in a constant-temperature magnetic stirrer, the stirring time is 1-1.5 hours, and the mixture is homogenized under the condition of 300kg/cm2 by a homogenizer.
4. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: in the step 5, the set temperature of the first extraction tank is 45 ℃, the extraction time is 35min, the stirring speed during extraction is 70r/min, the enzyme is immediately inactivated by a boiling water bath for 15min after the time is up, the set temperature of the second extraction tank is 50 ℃, the extraction time is 35min, the enzyme is not stirred during extraction, and the enzyme is immediately inactivated by the boiling water bath for 15min after the time is up.
5. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: the ultrafiltration in the step 6 is ceramic membrane ultrafiltration.
6. The segmented extraction method of the active ingredients of the moringa leaves according to claim 1, which is characterized in that: the power of ultrasonic extraction in the step 7 is 300W, the extraction temperature is 50 ℃, and the extraction time is 45 min.
7. A beverage containing the active ingredients of the moringa leaves is characterized in that water extract powder and ethanol extract powder extracted by the segmented extraction method of the active ingredients of the moringa leaves of any one of claims 1 to 6 are used for preparation, and the beverage comprises the following components in parts by weight:
10-20 parts of water extract powder, 5-10 parts of ethanol extract powder, 10-20 parts of medlar powder, 5-7 parts of sweet osmanthus powder, 8-10 parts of honeysuckle powder and 3-5 parts of liquorice powder.
8. The beverage containing the moringa oleifera active ingredient according to claim 7, wherein: the paint comprises the following components in parts by weight: 15 parts of water extract powder, 7 parts of ethanol extract powder, 15 parts of medlar powder, 6 parts of sweet osmanthus powder, 9 parts of honeysuckle powder and 4 parts of liquorice powder.
9. A method for preparing the beverage containing the active ingredients of the moringa oleifera leaves in claim 7 or 8, wherein the method comprises the following steps: the method comprises the following steps:
firstly, drying medlar, sweet osmanthus, honeysuckle and liquorice by hot air at 45 ℃ respectively, and crushing into powder;
mixing the water extract powder, the ethanol extract powder, the medlar powder, the sweet osmanthus powder, the honeysuckle powder and the liquorice powder in proportion, and uniformly stirring;
and thirdly, packaging the mixed powder according to 10g per package.
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| EP4707290A1 (en) | 2024-09-06 | 2026-03-11 | Vitarbo AG | Protein and fiber-containing green sheet material preparation and method for producing same |
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| EP4707290A1 (en) | 2024-09-06 | 2026-03-11 | Vitarbo AG | Protein and fiber-containing green sheet material preparation and method for producing same |
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