CN110628878B - 提高数字核酸扩增动态范围的ewod系统和方法 - Google Patents
提高数字核酸扩增动态范围的ewod系统和方法 Download PDFInfo
- Publication number
- CN110628878B CN110628878B CN201910495332.4A CN201910495332A CN110628878B CN 110628878 B CN110628878 B CN 110628878B CN 201910495332 A CN201910495332 A CN 201910495332A CN 110628878 B CN110628878 B CN 110628878B
- Authority
- CN
- China
- Prior art keywords
- sample
- droplet
- volume
- amplification
- ewod
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
- B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
- B01F33/30351—Micromixers using surface tension to mix, move or hold the fluids using hydrophilic/hydrophobic surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0427—Electrowetting
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16/014,006 US11198130B2 (en) | 2018-06-21 | 2018-06-21 | EWOD system and methods to increase dynamic range for digital nucleic acid amplification |
US16/014,006 | 2018-06-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110628878A CN110628878A (zh) | 2019-12-31 |
CN110628878B true CN110628878B (zh) | 2023-06-16 |
Family
ID=66776117
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910495332.4A Active CN110628878B (zh) | 2018-06-21 | 2019-06-06 | 提高数字核酸扩增动态范围的ewod系统和方法 |
Country Status (3)
Country | Link |
---|---|
US (1) | US11198130B2 (fr) |
EP (1) | EP3586965B1 (fr) |
CN (1) | CN110628878B (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11198130B2 (en) * | 2018-06-21 | 2021-12-14 | Sharp Life Science (Eu) Limited | EWOD system and methods to increase dynamic range for digital nucleic acid amplification |
CN113801923A (zh) * | 2021-10-20 | 2021-12-17 | 南京鼓楼医院 | 一种可直接用于血清标志物检测的数字滚环扩增检测方法 |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2570188A1 (fr) * | 2011-09-14 | 2013-03-20 | Sharp Kabushiki Kaisha | Dispositif à matrice active de régulation de fluide par électro-mouillage et diélectrophorèse et procédé d'entraînement |
WO2014043581A1 (fr) * | 2012-09-14 | 2014-03-20 | Life Technologies Corporation | Procédés et systèmes destinés à un concepteur d'expérience pcr numérique |
CN104450891A (zh) * | 2014-11-17 | 2015-03-25 | 中国科学院微生物研究所 | 基于微液滴的数字核酸扩增定量分析方法及系统 |
CN106460066A (zh) * | 2014-06-11 | 2017-02-22 | 赛普有限责任公司 | 通过液滴分选的核苷酸序列排除富集(needls) |
CN107666962A (zh) * | 2015-04-03 | 2018-02-06 | 雅培制药有限公司 | 用于样品分析的装置和方法 |
CN109863396A (zh) * | 2016-10-05 | 2019-06-07 | 雅培实验室 | 用于样品分析的装置和方法 |
EP3586965A1 (fr) * | 2018-06-21 | 2020-01-01 | Sharp Life Science (EU) Limited | Système et procédés ewod pour augmenter la plage dynamique d'amplification d'acide nucléique numérique |
CN110846388A (zh) * | 2018-08-21 | 2020-02-28 | 夏普生命科学(欧洲)有限公司 | 用于生物分析中的数字测定的微流控设备和方法 |
CN111569958A (zh) * | 2019-02-15 | 2020-08-25 | 夏普生命科学(欧洲)有限公司 | 通过使用ewod器件进行扩散的分子分离 |
WO2021048545A1 (fr) * | 2019-09-10 | 2021-03-18 | Oxford Nanopore Technologies Limited | Procédé, kit et système de synthèse de polynucléotides |
EP3812041A1 (fr) * | 2019-10-25 | 2021-04-28 | Sharp Life Science (EU) Limited | Procédé de fonctionnement d'un dispositif ewod avec appareil de détection |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6565727B1 (en) | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
US7163612B2 (en) | 2001-11-26 | 2007-01-16 | Keck Graduate Institute | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
WO2011020011A2 (fr) | 2009-08-13 | 2011-02-17 | Advanced Liquid Logic, Inc. | Actionneur à gouttelettes et techniques orientées gouttelettes |
WO2011057197A2 (fr) | 2009-11-06 | 2011-05-12 | Advanced Liquid Logic, Inc. | Actionneur de gouttelettes intégré pour électrophorèse sur gel et analyse moléculaire |
US8653832B2 (en) | 2010-07-06 | 2014-02-18 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
WO2015063767A1 (fr) | 2013-10-31 | 2015-05-07 | Yeda Research And Development Co. Ltd. | Synthèse de gènes et clonage acellulaire par microfluidique programmable |
US20180095067A1 (en) * | 2015-04-03 | 2018-04-05 | Abbott Laboratories | Devices and methods for sample analysis |
CA2982884C (fr) * | 2015-04-24 | 2021-03-30 | F. Hoffmann-La Roche Ag | Systemes et procedes de pcr numeriques utilisant la microfluidique numerique |
US9539573B1 (en) * | 2015-06-23 | 2017-01-10 | Sharp Kabushiki Kaisha | EWOD device with calibrated serial dilution function |
US11117134B2 (en) * | 2019-01-08 | 2021-09-14 | Sharp Life Science (Eu) Limited | Non-poissonian droplet partitioning using feedback |
-
2018
- 2018-06-21 US US16/014,006 patent/US11198130B2/en active Active
-
2019
- 2019-06-05 EP EP19178565.8A patent/EP3586965B1/fr active Active
- 2019-06-06 CN CN201910495332.4A patent/CN110628878B/zh active Active
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2570188A1 (fr) * | 2011-09-14 | 2013-03-20 | Sharp Kabushiki Kaisha | Dispositif à matrice active de régulation de fluide par électro-mouillage et diélectrophorèse et procédé d'entraînement |
WO2014043581A1 (fr) * | 2012-09-14 | 2014-03-20 | Life Technologies Corporation | Procédés et systèmes destinés à un concepteur d'expérience pcr numérique |
CN106460066A (zh) * | 2014-06-11 | 2017-02-22 | 赛普有限责任公司 | 通过液滴分选的核苷酸序列排除富集(needls) |
CN104450891A (zh) * | 2014-11-17 | 2015-03-25 | 中国科学院微生物研究所 | 基于微液滴的数字核酸扩增定量分析方法及系统 |
CN107666962A (zh) * | 2015-04-03 | 2018-02-06 | 雅培制药有限公司 | 用于样品分析的装置和方法 |
CN109863396A (zh) * | 2016-10-05 | 2019-06-07 | 雅培实验室 | 用于样品分析的装置和方法 |
EP3586965A1 (fr) * | 2018-06-21 | 2020-01-01 | Sharp Life Science (EU) Limited | Système et procédés ewod pour augmenter la plage dynamique d'amplification d'acide nucléique numérique |
CN110846388A (zh) * | 2018-08-21 | 2020-02-28 | 夏普生命科学(欧洲)有限公司 | 用于生物分析中的数字测定的微流控设备和方法 |
CN111569958A (zh) * | 2019-02-15 | 2020-08-25 | 夏普生命科学(欧洲)有限公司 | 通过使用ewod器件进行扩散的分子分离 |
WO2021048545A1 (fr) * | 2019-09-10 | 2021-03-18 | Oxford Nanopore Technologies Limited | Procédé, kit et système de synthèse de polynucléotides |
EP3812041A1 (fr) * | 2019-10-25 | 2021-04-28 | Sharp Life Science (EU) Limited | Procédé de fonctionnement d'un dispositif ewod avec appareil de détection |
Non-Patent Citations (4)
Title |
---|
Digital microfluidics: is a true lab-on-a-chip possible?;R. B. Fair;《Microfluid Nanofluid》;20070308;全文 * |
EWOD液体透镜动态响应测试与润湿机制研究;谢娜;《中国优秀博士学位论文全文数据库(电子期刊)》;20190115;全文 * |
一种基于数字微流控技术的PCR微芯片;牛嘉琦 等;《国外电子测量技术》;20170215;全文 * |
液体变焦透镜内芯的EWOD 特性的研究;栾辰 等;《科技天敌》;20100505;全文 * |
Also Published As
Publication number | Publication date |
---|---|
EP3586965A1 (fr) | 2020-01-01 |
CN110628878A (zh) | 2019-12-31 |
US20190388894A1 (en) | 2019-12-26 |
US11198130B2 (en) | 2021-12-14 |
EP3586965B1 (fr) | 2021-03-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107532128B (zh) | 使用数字微流体的数字pcr系统和方法 | |
US11915795B2 (en) | Method and device for digital high resolution melt | |
JP7490610B2 (ja) | 核酸分析および定量化のための方法ならびにシステム | |
CN110846388B (zh) | 用于生物分析中的数字测定的微流控设备和方法 | |
CN110628878B (zh) | 提高数字核酸扩增动态范围的ewod系统和方法 | |
US20230347353A1 (en) | Systems and methods for analyzing a biological sample | |
US20200384471A1 (en) | Microfluidic array for sample digitization | |
US20180258465A1 (en) | Methods and Devices for Performing Real Time Digital PCR | |
EP3353323B1 (fr) | Systèmes et procédés d'analyse d'acides nucléiques | |
Lee et al. | Development of a CCD-based fluorimeter for real-time PCR machine | |
US11351545B2 (en) | High speed nucleic acid melting analysis | |
Dhawan et al. | History of Digital PCR | |
KR20210145972A (ko) | 크리스퍼 진단법을 이용한 디지털 검출 방법 및 이의 장치 | |
García-Alonso et al. | Real-time Fluorescent Image Analysis of SlipChip-based Microfluidic Devices | |
JP2024502040A (ja) | 分析物分析のためのシステム及び方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: British Bridge Akers Applicant after: SHARP Kabushiki Kaisha Address before: oxford Applicant before: SHARP Kabushiki Kaisha |
|
GR01 | Patent grant | ||
GR01 | Patent grant |