CN110542749A - Landfill leachate toxicity detection method - Google Patents
Landfill leachate toxicity detection method Download PDFInfo
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- CN110542749A CN110542749A CN201910785436.9A CN201910785436A CN110542749A CN 110542749 A CN110542749 A CN 110542749A CN 201910785436 A CN201910785436 A CN 201910785436A CN 110542749 A CN110542749 A CN 110542749A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1031—Sampling from special places
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Abstract
the invention discloses a method for detecting the toxicity of landfill leachate, which specifically comprises the following steps: the method comprises the following steps: collecting the landfill leachate at the collection part of the landfill leachate guide pipe, wherein the collection is carried out for six times, the interval between every two collections is 20 minutes, after the collection is carried out for six times, all the collected leachate is poured into a plastic tank, holes are drilled on the periphery of the bottom of the plastic tank, after the leachate in the plastic tank is shaken, the leachate seeps out from the periphery of the plastic tank respectively, and the leachate is collected to obtain a final leachate sample; the invention relates to the technical field. According to the method for detecting the toxicity of the landfill leachate, the landfill leachate is collected at intervals in the step one, the collected leachate is collected after being mixed up, the reliability of a leachate sample can be greatly improved, meanwhile, two different environments of the clear water river leachate are adopted for contrast detection, and the persuasion of detection is greatly improved without being interfered by other environments.
Description
Technical Field
The invention relates to the technical field of landfill leachate, in particular to a method for detecting the toxicity of the landfill leachate.
Background
The landfill leachate is high-concentration organic wastewater which is formed by deducting the saturated water holding capacity of garbage and a soil covering layer from water contained in the garbage in a garbage landfill, rain, snow and water entering the landfill and other water and passing through the garbage layer and the soil covering layer. There is also water that accumulates to leak out of the waste ready for incineration. And an initial adjusting stage, namely filling the garbage into the landfill, and entering the initial adjusting stage at the stabilization stage of the landfill. In the stage, the easily degradable components in the garbage rapidly undergo aerobic biodegradation reaction with oxygen carried in the garbage to generate carbon dioxide (CO2) and water, and a certain amount of heat is released.
The standard of the existing landfill leachate detection method is not enough to be convinced, the detection structure is not accurate enough, whether other factors can influence the detection result or not can not be ensured, and the detection method is not comprehensive enough.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a landfill leachate toxicity detection method, and solves the problems that the calibration standard of the existing landfill leachate detection method is not enough to be convinced, the detection structure is not accurate enough, whether other factors can influence the detection result or not and the detection method is not comprehensive enough.
In order to achieve the purpose, the invention is realized by the following technical scheme: a method for detecting the toxicity of landfill leachate specifically comprises the following steps:
the method comprises the following steps: collecting the landfill leachate at the collection part of the landfill leachate guide pipe, wherein the collection is carried out for six times, the interval between every two collections is 20 minutes, after the collection is carried out for six times, all the collected leachate is poured into a plastic tank, holes are drilled on the periphery of the bottom of the plastic tank, after the leachate in the plastic tank is shaken, the leachate seeps out from the periphery of the plastic tank respectively, and the leachate is collected to obtain a final leachate sample;
Step two: uniformly and respectively placing two twenty hyphae in two different environments for growth, namely nontoxic clear water and an environment containing a percolate sample obtained in the step one;
step three: respectively observing the survival rates of the twenty hyphae under the two environments, and respectively observing and recording the survival rates once every two days;
step four: after ten days, statistics and analysis of data were carried out on survival of the silks in two different environments, and according to the formula: calculating the inhibition growth rate (%) (the diameter of a control colony-the diameter of a treated colony)/(the diameter of the control colony-the diameter of a bacterial cake) multiplied by 100 percent, calculating the inhibition growth rate of hyphae, taking the logarithm of a natural logarithm e of the concentration as an abscissa, and obtaining a pollutant toxicity intensity index by checking a conversion table of a biological statistic probability value;
step five: respectively putting substances X capable of precipitating with potential biological toxins in the percolate in two different environments, respectively taking out precipitates in the two environments, respectively weighing the mass of the two precipitates, and recording;
step six: and fifthly, calculating the recorded data, and deducing the indexes of the potential biological toxins in the leachate.
Preferably, the two hyphae in the second step and the third step are grown in a consistent manner in other environments, such as temperature, humidity and sunlight saturation.
The invention provides a method for detecting the toxicity of landfill leachate. The method has the following beneficial effects: the method for detecting the toxicity of the landfill leachate comprises the following steps: collecting the landfill leachate at the collection part of the landfill leachate guide pipe, wherein the collection is carried out for six times, the interval between every two collections is 20 minutes, after the collection is carried out for six times, all the collected leachate is poured into a plastic tank, holes are drilled on the periphery of the bottom of the plastic tank, after the leachate in the plastic tank is shaken, the leachate seeps out from the periphery of the plastic tank respectively, and the leachate is collected to obtain a final leachate sample; step two: uniformly and respectively placing two twenty hyphae in two different environments for growth, namely nontoxic clear water and an environment containing a percolate sample obtained in the step one; step three: the survival rates of the twenty hyphae under the two environments are respectively observed, the two hyphae are observed once every two days and are respectively recorded for recording,
through collecting landfill leachate at intervals in step one to collect collecting leachate after obscuring, can improve the reliability of leachate sample greatly, adopt clear water river leachate two kinds of different environment to contrast simultaneously and detect, and guarantee not to receive the interference of other environment and improved the persuasion that detects greatly.
Drawings
FIG. 1 is a table illustrating statistics of data under two different environments according to the present invention;
Detailed Description
the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, an embodiment of the present invention provides a technical solution: a method for detecting the toxicity of landfill leachate specifically comprises the following steps:
the method comprises the following steps: collecting the landfill leachate at the collection part of the landfill leachate guide pipe, wherein the collection is carried out for six times, the interval between every two collections is 20 minutes, after the collection is carried out for six times, all the collected leachate is poured into a plastic tank, holes are drilled on the periphery of the bottom of the plastic tank, after the leachate in the plastic tank is shaken, the leachate seeps out from the periphery of the plastic tank respectively, and the leachate is collected to obtain a final leachate sample;
Step two: uniformly and respectively placing two twenty hyphae in two different environments for growth, namely nontoxic clear water and an environment containing a percolate sample obtained in the step one;
step three: respectively observing the survival rates of the twenty hyphae under the two environments, and respectively observing and recording the survival rates once every two days;
Step four: after ten days, statistics and analysis of data were carried out on survival of the silks in two different environments, and according to the formula: calculating the inhibition growth rate (%) (the diameter of a control colony-the diameter of a treated colony)/(the diameter of the control colony-the diameter of a bacterial cake) multiplied by 100 percent, calculating the inhibition growth rate of hyphae, taking the logarithm of a natural logarithm e of the concentration as an abscissa, and obtaining a pollutant toxicity intensity index by checking a conversion table of a biological statistic probability value;
Step five: respectively putting substances X capable of precipitating with potential biological toxins in the percolate in two different environments, respectively taking out precipitates in the two environments, respectively weighing the mass of the two precipitates, and recording;
step six: and fifthly, calculating the recorded data, and deducing the indexes of the potential biological toxins in the leachate.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (2)
1. a method for detecting the toxicity of landfill leachate is characterized by comprising the following steps: the method specifically comprises the following steps:
The method comprises the following steps: collecting the landfill leachate at the collection part of the landfill leachate guide pipe, wherein the collection is carried out for six times, the interval between every two collections is 20 minutes, after the collection is carried out for six times, all the collected leachate is poured into a plastic tank, holes are drilled on the periphery of the bottom of the plastic tank, after the leachate in the plastic tank is shaken, the leachate seeps out from the periphery of the plastic tank respectively, and the leachate is collected to obtain a final leachate sample;
step two: uniformly and respectively placing two twenty hyphae in two different environments for growth, namely nontoxic clear water and an environment containing a percolate sample obtained in the step one;
Step three: respectively observing the survival rates of the twenty hyphae under the two environments, and respectively observing and recording the survival rates once every two days;
Step four: after ten days, statistics and analysis of data were carried out on survival of the silks in two different environments, and according to the formula: calculating the inhibition growth rate (%) (the diameter of a control colony-the diameter of a treated colony)/(the diameter of the control colony-the diameter of a bacterial cake) multiplied by 100 percent, calculating the inhibition growth rate of hyphae, taking the logarithm of a natural logarithm e of the concentration as an abscissa, and obtaining a pollutant toxicity intensity index by checking a conversion table of a biological statistic probability value;
step five: respectively putting substances X capable of precipitating with potential biological toxins in the percolate in two different environments, respectively taking out precipitates in the two environments, respectively weighing the mass of the two precipitates, and recording;
step six: and fifthly, calculating the recorded data, and deducing the indexes of the potential biological toxins in the leachate.
2. the method for detecting toxicity of landfill leachate according to claim 1, wherein the method comprises the following steps: the two hyphae in the second step and the third step grow to keep other environments consistent, such as temperature, humidity and sunlight saturation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910785436.9A CN110542749A (en) | 2019-08-23 | 2019-08-23 | Landfill leachate toxicity detection method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910785436.9A CN110542749A (en) | 2019-08-23 | 2019-08-23 | Landfill leachate toxicity detection method |
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| CN110542749A true CN110542749A (en) | 2019-12-06 |
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| CN201910785436.9A Withdrawn CN110542749A (en) | 2019-08-23 | 2019-08-23 | Landfill leachate toxicity detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115561417A (en) * | 2022-09-26 | 2023-01-03 | 安徽紫朔环境工程技术有限公司 | Leachate monitoring and early warning method for waste incineration power plant |
Citations (5)
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| CN102121935A (en) * | 2011-01-25 | 2011-07-13 | 宁波大学 | Method for detecting toxicity of characteristic toxic and harmful pollutants of aquatic products |
| CN102175606A (en) * | 2011-01-19 | 2011-09-07 | 西安建筑科技大学 | Method for detecting acute biological toxicity of sewage |
| CN105986000A (en) * | 2015-02-04 | 2016-10-05 | 中国科学院理化技术研究所 | Method for detecting biotoxicity of pollutant by mixed bacteria biosensor |
| CN106370633A (en) * | 2016-08-24 | 2017-02-01 | 中国环境科学研究院 | Measurement method of biodegradable humic acid in leachate |
| CN108120812A (en) * | 2017-12-14 | 2018-06-05 | 常州大学 | In a kind of dyeing waste water in groups biological toxicity tests and differentiate appraisal procedure |
-
2019
- 2019-08-23 CN CN201910785436.9A patent/CN110542749A/en not_active Withdrawn
Patent Citations (5)
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| CN102175606A (en) * | 2011-01-19 | 2011-09-07 | 西安建筑科技大学 | Method for detecting acute biological toxicity of sewage |
| CN102121935A (en) * | 2011-01-25 | 2011-07-13 | 宁波大学 | Method for detecting toxicity of characteristic toxic and harmful pollutants of aquatic products |
| CN105986000A (en) * | 2015-02-04 | 2016-10-05 | 中国科学院理化技术研究所 | Method for detecting biotoxicity of pollutant by mixed bacteria biosensor |
| CN106370633A (en) * | 2016-08-24 | 2017-02-01 | 中国环境科学研究院 | Measurement method of biodegradable humic acid in leachate |
| CN108120812A (en) * | 2017-12-14 | 2018-06-05 | 常州大学 | In a kind of dyeing waste water in groups biological toxicity tests and differentiate appraisal procedure |
Non-Patent Citations (4)
| Title |
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| 曾晓岚 等: "发光细菌法在渗滤液生物毒性测定中的应用", 《环境科学与技术》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115561417A (en) * | 2022-09-26 | 2023-01-03 | 安徽紫朔环境工程技术有限公司 | Leachate monitoring and early warning method for waste incineration power plant |
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