CN1104861A - Pollen broken wall and nutrient content extracting technology - Google Patents
Pollen broken wall and nutrient content extracting technology Download PDFInfo
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- CN1104861A CN1104861A CN93104588A CN93104588A CN1104861A CN 1104861 A CN1104861 A CN 1104861A CN 93104588 A CN93104588 A CN 93104588A CN 93104588 A CN93104588 A CN 93104588A CN 1104861 A CN1104861 A CN 1104861A
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- pollen
- broken wall
- homogenate
- colloid mill
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Abstract
The intine and extine of pollen are broken through physical method and the refined pollen nutritive liquid is extracted through centrifugal filtration processing. The whole course is undergone under relative low temp environment. It has the advantage of completely breaking of intine and extine meanwhile the results of sterilizing and settlement are achieved. The pollen nutritive liquid is light yellow in colour, clear and transparent and possesses of the advantages of unique fragrance, nourishing and easy absorbed by humanbody.
Description
The present invention is a kind of about pollen broken wall and nutrition extraction new technology.
Pollen is phanerogamic male gametophyte, contains bioactivators such as abundant nutrition and enzyme, has medical treatment and health care, is a kind of natural food.But pollen wall is made up of hard sporopollenin, and is directly edible without the pollen of broken wall, not only has peculiar smell, and is difficult to fully be digested and assimilated by human body, is unfavorable for the development and use to pollen product.
Before the present invention, seen the report of pollen wall breaking technology, the general methods such as enzymolysis broken wall alternating temperature broken wall, mechanical breaking-wall method, freezing broken wall that adopt, but all there are some complex process, production efficiency is not high, shortcomings such as sporoderm-broken rate is low, being 85106076 patents state as the patent No., a kind of " pollen wall breaking technology " is not suitable for suitability for industrialized production, cost is higher, bioactivator is preserved complete inadequately, various bacteriums still can remain in the pollen material behind its broken wall, to shortcomings such as the easy inactivations of bioactivator.
The object of the present invention is to provide a kind of broken wall fully, and can have the characteristics that keep the natural pollen color, and can substitute sterilization, sterilization, and can keep bioactivator.
Pollen freeze thawing broken wall new technology provided by the present invention is characterized in pollen grain through behind the water logging multigelation, it is expanded shrink and morphosis changes.Also changed simultaneously inside and outside osmotic pressure and the concentration difference of pollen, pass through the grinding pressurized treatments of colloid mill again, extine fragmentation or germinal aperature ditch are extremely expanded broken, the pollen behind the broken wall is extracted refining pollen nutrient liquid through sheet frame pressure, filter and milipore filter again through the rough stoste that Separation of Solid and Liquid goes out.
A kind of pollen broken wall provided by the invention with extract nutrient solution new technology, its technology simple, be easy to grasp, the production efficiency height, broken wall reaches 98% fully, bioactivator is difficult for by the advantage of inactivation, is fit to suitability for industrialized production again.Simultaneously owing to adopted the ultrafiltration membrance filter technology, can extract through the refining pollen nutrient liquid after sterilization and the clarification, its fragrance is arranged, nutritiously easily directly be absorbed by human body, be a kind of pure natural high-grade nutrient health care food, also opened up wide prospect more for the development and use pollen product.
Be embodiments of the invention below:
1, learn from else's experience 5 kilograms in the selected raw material melissa powder of removing impurity adds 10 liters of distilled water, stirs to be placed in-20 ℃ of refrigerator-freezers or the freezer freezing 12-24 hour.
2, freezing pollen is placed under the normal temperature, melt through the running water circulation at its external container, placed-20 ℃ of refrigerator-freezers or the freezing 12-24 of freezer hour again, so multigelation is 3-4 time.
3, freezer being put in the above-mentioned homogenate of pollen after colloid mill repeated multiple times circular treatment through freeze thawing stores.
4, the pollen behind the broken wall by centrifuge with Separation of Solid and Liquid, collect rough aqueous extract (I) and will consolidate the shape residue again with 50% alcohol immersion sepage, remove ethanol and obtain ethanol extract (II), I, II two liquid are merged, through plate compression, select for use 1.5 ‰ milipore filters to extract and make with extra care pollen nutrient liquid or make crystalline powder again through freeze drying.
Claims (3)
1, a kind of pollen broken wall and nutrition extractive technique, its step is a pollen of removing impurity with cleaning, is soaked in water, freeze thawing, colloid mill, homogenate, centrifugation go out pollen, handle through membrane filtration again, extract refining pollen nutrient liquid.
2, by the described process for breaking wall of pollen of claim 1, it is characterized in that adopting the colloid mill broken shell to circulate repeatedly repeatedly.
3, by the described nutrition extractive technique of claim 1, it is characterized in that separating solid-liquid through freeze thawing, homogenate, colloid mill, homogenate, centrifuge, pass through 31.5 ‰ ultrafiltration membrance filter after the rough stoste of collection pollen again.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN93104588A CN1104861A (en) | 1993-04-17 | 1993-04-17 | Pollen broken wall and nutrient content extracting technology |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN93104588A CN1104861A (en) | 1993-04-17 | 1993-04-17 | Pollen broken wall and nutrient content extracting technology |
Publications (1)
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CN1104861A true CN1104861A (en) | 1995-07-12 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN93104588A Pending CN1104861A (en) | 1993-04-17 | 1993-04-17 | Pollen broken wall and nutrient content extracting technology |
Country Status (1)
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CN (1) | CN1104861A (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101971939A (en) * | 2010-06-23 | 2011-02-16 | 安徽万山生物科技有限公司 | Process for making bee pollen freeze-dried powder through low-temperature high-pressure wall-breaking |
CN101971940A (en) * | 2010-06-23 | 2011-02-16 | 安徽万山生物科技有限公司 | Process for preparing low-temperature high-pressure wall-breaking pine pollen frozen powder capsule |
CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
CN102511706A (en) * | 2011-12-26 | 2012-06-27 | 重庆美滋滋生物科技有限公司 | Pollen honey manufacturing method |
CN101332219B (en) * | 2007-06-29 | 2012-06-27 | 浙江海正药业股份有限公司 | Broken pollen extract and extracting method and use thereof |
CN102793095A (en) * | 2012-08-21 | 2012-11-28 | 中国科学院西北高原生物研究所 | Rape bee pollen homogenizing wall-breaking method |
CN105866352A (en) * | 2016-06-08 | 2016-08-17 | 环境保护部南京环境科学研究所 | Method for evaluating bee toxicology effect of transgenic plant pollen, pollen extracting liquid and preparation method thereof |
-
1993
- 1993-04-17 CN CN93104588A patent/CN1104861A/en active Pending
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101332219B (en) * | 2007-06-29 | 2012-06-27 | 浙江海正药业股份有限公司 | Broken pollen extract and extracting method and use thereof |
CN101971939A (en) * | 2010-06-23 | 2011-02-16 | 安徽万山生物科技有限公司 | Process for making bee pollen freeze-dried powder through low-temperature high-pressure wall-breaking |
CN101971940A (en) * | 2010-06-23 | 2011-02-16 | 安徽万山生物科技有限公司 | Process for preparing low-temperature high-pressure wall-breaking pine pollen frozen powder capsule |
CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
CN102229973B (en) * | 2011-06-15 | 2013-05-01 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
CN102511706A (en) * | 2011-12-26 | 2012-06-27 | 重庆美滋滋生物科技有限公司 | Pollen honey manufacturing method |
CN102793095A (en) * | 2012-08-21 | 2012-11-28 | 中国科学院西北高原生物研究所 | Rape bee pollen homogenizing wall-breaking method |
CN105866352A (en) * | 2016-06-08 | 2016-08-17 | 环境保护部南京环境科学研究所 | Method for evaluating bee toxicology effect of transgenic plant pollen, pollen extracting liquid and preparation method thereof |
CN105866352B (en) * | 2016-06-08 | 2018-05-04 | 环境保护部南京环境科学研究所 | Genetically modified plants pollen is evaluated to the method for honeybee toxicological effect and pollen extracting liquid and preparation method thereof |
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