CN110464867A - It is a kind of that peripheral nerve reparation and wound healing is promoted to merge the Piezoelectric anisotropy dressing and preparation method of load Chinese medicine excretion body - Google Patents
It is a kind of that peripheral nerve reparation and wound healing is promoted to merge the Piezoelectric anisotropy dressing and preparation method of load Chinese medicine excretion body Download PDFInfo
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Abstract
The present invention provides Piezoelectric anisotropy dressing and preparation method that a kind of promotion peripheral nerve reparation and wound healing merge load Chinese medicine excretion body, comprising the following steps: gelatin and methacrylic acid anhydride reactant are prepared methacrylated gelatin；Chinese medicine excretion body is prepared by differential centrifugation etc.；Gold nanorods, excretion body and methacrylated is gelatin-compounded, prepare conductive pregel system；Conductive pregel is compound with poly meta fluoroethylene piezoelectric film, it is prepared into Piezoelectric anisotropy dressing.Dressing prepared by the present invention is a kind of new pattern compress that piezoelectricity stimulation combination drug acts on, Kynoar generates potential and passes through Signa Gel, wound area is stimulated, it cooperates with Chinese medicine excretion body to promote wound healing and nerve regneration, can effectively solve the problem during current wound repair and neural restoration.Therefore, combine dressing of the present invention is with a wide range of applications in the treatment of wound and neural restoration.
Description
Technical field
The Piezoelectric anisotropy for merging load Chinese medicine excretion body the present invention relates to a kind of promotion peripheral nerve reparation and wound healing applies
Material and preparation method.
Background technique
Skin is the first line of defence and the maximum organ of human body of human body, has and the multiple functions such as feels, protects.So
And in daily life, skin suffers from the influence of a variety of extraneous factors, usually causes wound, and it is heavy to bring to a people and society
The pressure and burden of weight.However wound healing is a complexity and dynamic process, including Various Tissues and cell etc. are joined jointly
With adjusting.And the uncontrolled adjusting of wound repair process, cause after wound healing scar is serious, accessory organ cannot be newborn,
It causes to repair obstacle.Meanwhile skin surface is also dispersed with complicated neural network, mainly includes sensory nerve, autonomic nerve etc.
Different nervous systems, they play the various functions such as perception and Physiological effect jointly.Serious skin injury can destroy skin
The receptor of skin and the nerve fibre of associated system, lead to the missing of different skin sensory function.
Peripheral nerve repair is a kind of common clinical problem, and spontaneous Peripheral nerve repair is almost always incomplete,
And functional rehabilitation is poor.After peripheral nerve injury leads to neurologic defect, especially when there are biggish neural gaps (in human body
20mm or longer) when, suture neural residul end is difficult to carry out, and generally requires to be inserted into some form of graft between neural residul end
For bridging gap and supporting axon regeneration.It is the self mind of implantation currently used for the goldstandard therapy that peripheral nerve gap is repaired
It is usually the nerve segment not too important from another position portability function of body through graft.However, autologous nerve moves
Plant haves the shortcomings that intrinsic, and the supply including donor nerve is limited, needs to carry out second to perform the operation, donor nerve and receptor site
Between mismatch and institutional framework and the potential difference of size etc..
Mesenchymal stem cell is a kind of pluripotent cell, has the potentiality for being divided into various cell types, including fat
Cell, endothelial cell, osteoblast and cartilage cell and nerve cell etc..And due to mesenchymal stem cell abundance
And be easily isolated, mescenchymal stem cell is considered as one of most important source of human stem cell of regenerative medicine, and is also widely used for
Cell therapy and organizational project.However the stem cell of nature cannot be divided into specific Neural lineage cells type, but
It is influenced by some external factors, such as chemical growth factors, extracellular matrix components and electro photoluminescence etc., medulla mesenchyma
Stem cell can promote Peripheral nerve repair with differentiating into nerve cells.
Human body cell is inside and outside, and there is the ions of different kinds and concentrations.When skin damage, different ion on wound
It is different that gradient can generate epithelial voltage clamp, then generates endothelium electric field, and remains to the completion of skin regeneration process.Endothelium electric field is not
Skin Cell behavior is only adjusted in wound healing process, further promotes the regeneration of skin.Now studies have found that endothelium electric field can
It stimulates fibroblast proliferation and is divided into myofibroblast, keratinocyte cell migration and angiogenesis.
Electro photoluminescence can promote mesenchymal stem cells into neurons to break up, and can also promote the healing of wound.Therefore, conductive branch
Frame, conductive dressing etc., also gradually pay close attention to for researcher.On the one hand wherein conductive hydrogel has outstanding biocompatibility
With certain mechanical strength；On the one hand drug can also be contained, and passes through the release drug such as erosion or degradation under electro photoluminescence.This
A little characteristics make Signa Gel become outstanding pharmaceutical carrier.However, traditional Signa Gel often compound polypyrrole, carbon nanotube
Equal conducting polymers, it tends to be difficult to degrade.
The dressing of the conduction such as Signa Gel in use, needs that external equipment is combined to carry out electro photoluminescence.Most of electricity thorns
Swash building for equipment, external energy input is often needed to connect with conducting wire, it is complex.On the one hand, this increase treatment the step of and
On the other hand the requirement for the treatment of also brings additional economy and psychological burden to patient.Therefore, the electricity of no external equipment
Perhaps, stimulation therapy can clinically be used widely.
Piezoelectric material, be under pressure in a certain direction or pulling force effect and when deforming, internal positive and negative charge center
Polarization phenomena are generated with respect to transfer, can be generated charge on surface, then be generated potential difference.Piezoelectric material is usually used in sensing
Device, the fields such as generator, wherein most importantly inorganic piezoelectric material, the piezoelectric ceramics including the preparation of the materials such as PZT, calcium mine titanium
Deng.However as the study found that organic material also has these performances.
On the one hand Kynoar (Kynoar) and its copolymer have extremely strong piezoelectric effect, comparable to traditional
Inorganic piezoelectric material, while being organic matter again, have shown that there is good biocompatibility and mechanical performance through research.It is existing to grind
Study carefully, mainly using its potential difference for the stimulation of cell.However, being often difficult to reach by the performance of material itself merely
Good therapeutic effect.In clinical use, it is also necessary to the synergistic effect with drug, however the research in terms of the field, mesh
Preceding is still blank.
Summary of the invention
The pressure of a kind of promotion peripheral nerve reparation and wound healing merging load Chinese medicine excretion body is provided when the purpose of the present invention
Electric combine dressing and preparation method, to solve:
A. skin regeneration and nerve regneration problem in wound healing process；
B. electro photoluminescence needs external equipment problem；
C. piezoelectricity stimulation and drug synergism.
In order to achieve the above object, present invention employs following technical proposals:
The present invention discloses the piezoelectricity of a kind of promotion peripheral nerve reparation and wound healing merging load Chinese medicine excretion body first
The preparation method of combine dressing, comprising the following steps:
A. gelatin and methacrylic acid anhydride reactant are prepared into methacrylated gelatin；
B. it by differential centrifugation, density-gradient centrifugation method, prepares and purifies Chinese medicine excretion body；
C. methacrylated gelatin is dissolved in ultrapure water or phosphate buffered saline solution, then by gold nanorods and
The Chinese medicine excretion body and methacrylated of preparation are gelatin-compounded, and adulterate photosensitizer, are prepared into conductive pregel system；D.
Conductive pregel is spun on poly meta fluoroethylene piezoelectric film, then by photo-crosslinking, is prepared into combine dressing.As this hair
Bright preferred embodiment, in the step A, the grafting rate of methacrylated gelatin is 20-90%.
As a preferred solution of the present invention, in the step B, the source medicinal material of excretion body be ginseng, the fruit of glossy privet, Radix Salviae Miltiorrhizae,
One of glutinous rehmannia, west safflower, Chinese herbaceous peony are a variety of.
As a preferred solution of the present invention, the step B specifically:
1) medicinal material cleaned, then squeezed the juice；The juice squeezed crosses the sieve of 100-300 mesh, removes medicinal material residue；It will sieving
Juice carry out differential centrifugation, first centrifugal force be 1000-10000g under, be centrifuged 10-60 minutes, discard precipitating；Then will
Supernatant is centrifuged 10-120 minutes in the case where centrifugal force is 3000-30000g；Discard precipitating, by supernatant again centrifugal force be 10000-
Under 300000g, it is centrifuged 10-480 minutes；
2) precipitating for obtaining step 1) final step is resuspended with ultrapure water or phosphate buffered saline solution, is then passed through
Density gradient centrifugation purifying, density gradient solution are prepared by sucrose or CsCl, and each gradient solution concentration is 10-90%；Centrifugation
Power 100000-200000g, time are 10-360 minutes；
3) the excretion body in various concentration section is sucked out, is centrifuged at centrifugal force 50000-300000g, time 10-360
Minute, it is precipitated.It is resuspended with ultrapure water or phosphate buffered saline solution, obtains Chinese medicine excretion body.
As a preferred solution of the present invention, the draw ratio of the gold nanorods is 1-5.
As a preferred solution of the present invention, in the conductive pregel system of the step C, methacrylated gelatin
Mass percent concentration is 5%-30%；The concentration of nanometer rods is 0.1-5mg/ml；The concentration of Chinese medicine excretion body is 1ng-10ug/
ml；Photosensitizer is one or both of LAP and I2959.
As a preferred solution of the present invention, the poly meta fluoroethylene piezoelectric film the preparation method comprises the following steps: passing through rotary coating
Method coating, is then annealed, polarizing is prepared into poly meta fluoroethylene piezoelectric film；Spin coating rear film with a thickness of 10-50 μm, move back
For the temperature of fire between 60-150 DEG C, poling temperature is 50-150 DEG C, and voltage is 80-200V/ μm, and the polarization time is 1-8 hours.
As a preferred solution of the present invention, in the step D, in conductive pregel system, the photo-crosslinking time is 10-360
Second, methacrylated gelatin is after spin coating with a thickness of 1-5mm.
The invention also discloses combine dressings made from the method.
The invention also discloses the combine dressings in intelligent response material, wound healing and neural restoration field
Using.
Due to the adoption of the above technical scheme, the present invention achieve it is following the utility model has the advantages that
1. the present invention is applied widely, do not need to reduce production cost using special material；
2. Signa Gel prepared by the present invention, there is good electric conductivity and mechanical strength, it is degradable and there is sustained release to make
With, and in vitro experiment, the cell adhesion ability and biocompatibility having；
3. Chinese medicine excretion body prepared by the present invention, Chinese medicine is derived from, comes that source range is wide, and preparation is easy, yield is high, has wide
Big medical and commercial prospect.And in vitro experiment, it was demonstrated that Proliferation of Bone Mesenchymal Stem Cells can be effectively promoted, moved
It moves, and promotes mesenchymal stem cell as neural class cell is broken up；
4. combine dressing prepared by the present invention can generate electro photoluminescence by itself, and contain release excretion body.One side
Face can induce stem cell to migrate to wound, acted on by excretion body and promote stem cell differentiation；On the one hand, Kynoar
The potential of generation is applied on stem cell by the conduction of Signa Gel, provides electro photoluminescence, and collaboration excretion body promotes dry together
Cell differentiating into nerve cells, and promote wound healing.
Detailed description of the invention
Fig. 1 is the hydrogen nuclear magnetic resonance spectrogram of gelatin and methacrylated gelatin；
Fig. 2 is the cell adhesion result figure of combine dressing；
Fig. 3 is the uv scan figure of gold nanorods；
Fig. 4 is the transmission electron microscope results figure of gold nanorods；
Fig. 5 is the dead coloration result figure of work of combine dressing；
Fig. 6 is the partial size potential diagram of excretion body；
Fig. 7 is the transmission electron microscope results figure of excretion body；
Fig. 8 is stem cell 1 day CCK-8 proliferation results figure after the administration of excretion body；
Fig. 9 is nestin (Nestin) the immunofluorescence results figure of stem cell after excretion body is administered 7 days；
Figure 10 is the rat wound healing result figure of combine dressing；
Figure 11 is the rat body weight result figure of combine dressing；
Figure 12 is rat skin neurofilament protein (NF-L) ImmunohistochemistryResults Results figure of combine dressing.
Specific embodiment
With reference to the accompanying drawing, the present invention is described in detail.
It is clear in order to be more clear the purpose of the present invention, technical solution and advantage, below in conjunction with drawings and examples,
The present invention will be described in further detail.Specific embodiment described herein is not used to limit for explaining only the invention
The fixed present invention.
Embodiment 1: a kind of piezoelectricity-Signa Gel promoting peripheral nerve reparation and the combine dressing for loading Chinese medicine excretion body
And preparation method thereof, comprising the following steps:
1) it by gelatin and methacrylic acid anhydride reactant, is dialysed 1 week with room temperature in the bag filter of 8k-14kDa, is then frozen later
It is dry, it is prepared into methacrylated gelatin；
2) first glossy privet is cleaned, juicing sieves with 100 mesh sieve；Again by differential centrifugation, successively with 1000g, 12000g from
After the heart, supernatant is taken to be centrifuged at 100000g with ultracentrifuge, then precipitating is resuspended with PBS.Density is equipped with sucrose
Then gradient solution surpassed at 200000g from 60 minutes；The excretion body for taking different component again is centrifuged 60 minutes at 50000g,
It is prepared into Chinese medicine excretion body；
3) pass through HAuCl4With CTAB, NaBH4It is prepared into seed solution, HAuCl4、CTAB、AgNO3, the Dutch treatment is for Cheng Shengchang
Solution, then by seed mediated growth method, in 25 DEG C, growth overnight, is prepared into gold nanorods；
4) methacrylated gelatin is dissolved with PBS, its concentration is made to reach 5%；Then gold nanorods are added, make it
Concentration reaches 1mg/ml；Chinese medicine excretion body is added, its ultimate density is made to reach 10 μ g/ml.It is blended into photosensitizer LAP, is obtained
Glue pregel system；
5) Kynoar powder monomer is taken, after being dissolved with anhydrous DMF, then rotary coating is annealed for 120 DEG C at film,
After annealing, 100 DEG C, under 120V/ μm of electric field, polarizes 8 hours, Kynoar is prepared, with a thickness of 10 μm；
6) it by hydrogel pregel system, is spun on Kynoar, then photo-crosslinking 60s, is prepared into combine dressing,
Wherein hydrogel thickness is 2mm；
It after preparing methacrylated gelatin, is dissolved in deuterated water, is then tested with Nuclear Magnetic Resonance, obtain phase
Close material1H-NMR spectrum determines the chemical structure of methacrylated gelatin；As shown in Figure 1, compared to gelatin, methyl
The peak (shown in black dotted lines frame) that there are chemical shifts between 5.2~5.6ppm in the H spectrum of acroleic acid esterification gelatin, represents
Hydrogen atom on double key carbon.And it is influenced by shadow shield effect and long range shielding effect, two on double key carbon hydrogen atom
It is in different chemical shifts
After being prepared into combine dressing, by combine dressing in super-clean bench ultraviolet irradiation 0.5 hour.It is dry thin with medulla mesenchyma
Born of the same parents' complete culture solution washes away alcohol and impregnates hydrogel, balances in 37 DEG C.By the mesenchymal stem cell of digestion with 1 ×
105The cell density of/mL is seeded on gel, is placed in 37 DEG C, 5%CO2Incubator in culture 24 hours after, sop up culture medium,
PBS is cleaned twice, then is impregnated and fixed after ten minutes with 4% paraformaldehyde, washs 2 with the PBS of the X-100 containing 0.1%Triton
It is secondary, about 5 minutes every time.With the PBS containing 5%BSA and 0.1%Triton X-100 according to the dilution proportion Actin- of 1:100
Tracker Green dyeing liquor, is added drop-wise on cell, and room temperature is washed twice with PBS again after being protected from light incubation 1h.500 μ l/ml are added
DAPI solution is incubated at room temperature 15 minutes, is removed DAPI solution and is immediately placed under Laser Scanning Confocal Microscope and sees after PBS cleaning twice
It examines.As shown in Fig. 2, cell normally sticks on the hydrogel, and cellular morphology is normal, stretches at fusiform after culture for 24 hours.
Embodiment 2: a kind of piezoelectricity-Signa Gel promoting peripheral nerve reparation and the combine dressing for loading Chinese medicine excretion body
And preparation method thereof, comprising the following steps:
1) it by gelatin and methacrylic acid anhydride reactant, is dialysed 2 weeks with room temperature in the bag filter of 8k-14kDa, is then frozen later
It is dry, it is prepared into methacrylated gelatin；
2) first Radix Salviae Miltiorrhizae is cleaned, 200 meshes are crossed in juicing；Again by differential centrifugation, successively with 2000g, 10000g from
After the heart, supernatant is taken to be centrifuged at 120000g with ultracentrifuge, then precipitating is resuspended with PBS.It is molten with CsCl gradient
Then liquid surpassed at 100000g from 60 minutes；The excretion body for taking different component again is centrifuged 60 minutes at 100000g, preparation
At Chinese medicine excretion body；
3) pass through HAuCl4With CTAB, NaBH4It is prepared into seed solution, HAuCl4、CTAB、AgNO3, the Dutch treatment is for Cheng Shengchang
Solution, then by seed mediated growth method, in 28 DEG C, growth overnight, is prepared into gold nanorods；
4) methacrylated gelatin is dissolved with PBS, its concentration is made to reach 10%；Then gold nanorods are added, make
Its concentration reaches 2mg/ml；Chinese medicine excretion body is added, its ultimate density is made to reach 1 μ g/ml.
It is blended into photosensitizer LAP, obtains glue pregel system；
5) Kynoar powder monomer is taken, after being dissolved with anhydrous DMF, then rotary coating is annealed for 100 DEG C at film,
After annealing, 130 DEG C, under 120V/ μm of electric field, polarizes 4 hours, Kynoar is prepared, with a thickness of 20 μm；
6) it by hydrogel pregel system, is spun on Kynoar, then photo-crosslinking 30s, is prepared into combine dressing,
Wherein hydrogel thickness is 5mm；
The gold nanorods that preparation is completed first pass through the test of UV-vis absorption spectrum instrument, determine that gold nanorods exist
The absorption peak of 350-900nm；As shown in figure 3, gold nanorods altogether there are two absorption peak, respectively correspond the major diameter of gold nanorods and short
Diameter.
Then by gold nanorods solution, drop is on the copper mesh for being coated with carbon film, and drop passes through transmission on copper mesh after natural drying
Electronic Speculum observes its pattern.As shown in Figure 4: gold nanorods are in the club shaped structure of standard, and pattern is uniform, favorable dispersibility in water, warp
Measurement, gold nanorods draw ratio is about 4.2.
After being prepared into combine dressing, alcohol is washed away with mesenchymal stem cell complete culture solution and impregnates hydrogel, in
It is balanced in 37 DEG C.The mesenchymal stem cell of digestion is seeded on gel with the cell density of 1 × 105/mL, is placed in 37
DEG C, in the incubator of 5%CO2 after culture 48h, remove culture solution, PBS is cleaned 3 times.Addition is diluted to 2 μM and 8m respectively with PBS
Calcein AM and the PI solution of Μ, did not had cell, was protected from light incubation 30-45 minutes, was finally cleaned three times with PBS, by remnants'
Sample to be tested, is placed under laser confocal microscope observes immediately by reagent removal.Wherein living cells display is green, in dead cell
Nucleic acid substances be dyed to red.As shown in figure 5, cell shows green substantially in combine dressing after culture 48h, have no red, card
Bright combine dressing has good biocompatibility.
Embodiment 3: a kind of piezoelectricity-Signa Gel promoting peripheral nerve reparation and the combine dressing for loading Chinese medicine excretion body
And preparation method thereof, comprising the following steps:
1) by gelatin and methacrylic acid anhydride reactant, room temperature is dialysed 1 week in the bag filter of 8k-14kDa later, is then frozen
It is dry, it is prepared into methacrylated gelatin；
2) first glutinous rehmannia is cleaned, squeezes the juice, cross 150 meshes；Again by differential centrifugation, successively it is centrifuged with 2000g, 10000g
Later, it takes supernatant to be centrifuged at 150000g with ultracentrifuge, then precipitating is resuspended with PBS.With Sucrose gradient solutions,
Then surpass at 150000g from 120 minutes；The excretion body for taking different component again is centrifuged 60 minutes at 150000g, is prepared into
Chinese medicine excretion body；
3) pass through HAuCl4With CTAB, NaBH4It is prepared into seed solution, HAuCl4、CTAB、AgNO3, the Dutch treatment is for Cheng Shengchang
Solution, then by seed mediated growth method, in 30 DEG C, growth overnight, is prepared into gold nanorods
4) its concentration is made to reach 20% the dissolution of methacrylated gelatin ultrapure water；Then gold nanorods are added,
Its concentration is set to reach 2mg/ml；Chinese medicine excretion body is added, its ultimate density is made to reach 1 μ g/ml.It is blended into photosensitizer I2959,
Obtain glue pregel system；
5) Kynoar powder monomer is taken, after being dissolved with anhydrous DMF, then rotary coating is annealed for 120 DEG C at film,
After annealing, 115 DEG C, under 90V/ μm of electric field, polarize 8h, Kynoar is prepared, with a thickness of 20 μm；
6) it by hydrogel pregel system, is spun on Kynoar, then photo-crosslinking 60s, is prepared into combine dressing,
Wherein hydrogel thickness is 3mm；
The excretion body that preparation is completed is analyzed into its partial size current potential in Malvern particle size analyzer.As shown in fig. 6, excretion
The partial size and Potential distribution of body are uniform, wherein partial size be 144.1 ± 2.762nm, PDI 0.218, surface potential be -27.4 ±
0.451mV。
Then excretion liquid solution is dripped on the copper mesh for being coated with carbon film, is redyed later with uranium acetate, transmission electricity
Sem observation.As shown in fig. 7, excretion body is in standard pie structure.
The mesenchymal stem cell of the third generation is taken, with 5 × 103The density kind in/hole is cultivated 24 hours on 96 orifice plates,
The excretion body of various concentration is added into 96 orifice plates.It is put into after being incubated for 6 hours in cell incubator, culture solution is sucked out, PBS washes two
It is secondary, after being changed to the fresh fresh medium containing 10% serum by culture solution and continue culture 18 hours, culture solution is removed,
The 100 μ L of DMEM low sugar culture solution containing CCK-8 is added, is put into cell incubator culture 4 hours, is surveyed at microplate reader 475nm
Determine light absorption value.Calculate cell Proliferation percentage.As shown in figure 8, three kinds it is different organize other excretion bulk concentration cultures under, from low dense
High concentration is spent, excretion body has good proliferation function to mesenchymal stem cell, and proliferation rate is gradually increasing.
Embodiment 4: a kind of piezoelectricity-Signa Gel promoting peripheral nerve reparation and the combine dressing for loading Chinese medicine excretion body
And preparation method thereof, comprising the following steps:
1) by gelatin and methacrylic acid anhydride reactant, room temperature is dialysed 2 weeks in the bag filter of 8k-14kDa later, is then frozen
It is dry, it is prepared into methacrylated gelatin；
2) first ginseng is cleaned, 200 meshes are crossed in juicing；Again by differential centrifugation, successively with 3000g, 10000g from
After the heart, supernatant is taken to be centrifuged at 150000g with ultracentrifuge, then precipitating is resuspended with PBS.It is molten with saccharose gradient
Then liquid surpassed at 100000g from 120 minutes；The excretion body for taking different component again is centrifuged 60 minutes at 30000g, preparation
At Chinese medicine excretion body；
3) pass through HAuCl4With CTAB, NaBH4It is prepared into seed solution, HAuCl4、CTAB、AgNO3, the Dutch treatment is for Cheng Shengchang
Solution, then by seed mediated growth method, in 25 DEG C, growth overnight, is prepared into gold nanorods
4) its concentration is made to reach 10% the dissolution of methacrylated gelatin ultrapure water；Then gold nanorods are added,
Its concentration is set to reach 3mg/ml；Chinese medicine excretion body is added, its ultimate density is made to reach 2 μ g/ml.It is blended into photosensitizer LAP, is obtained
To glue pregel system；
5) Kynoar powder monomer is taken, after being dissolved with anhydrous DMF, then rotary coating is annealed for 120 DEG C at film,
After annealing, 100 DEG C, under 120V/ μm of electric field, polarize 8h, Kynoar is prepared, with a thickness of 30 μm；
6) it by hydrogel pregel system, is spun on Kynoar, then photo-crosslinking 120s, is prepared into compound apply
Material, wherein hydrogel thickness is 5mm；
By composite auxiliary material in super-clean bench, ultraviolet irradiation 0.5 hour, washed away with mesenchymal stem cell complete culture solution
Alcohol simultaneously impregnates hydrogel, balances in 37 DEG C.By the mesenchymal stem cell of digestion with 1 × 105The cell density of/mL connects
Kind is placed in 37 DEG C, 5%CO on gel2Incubator in every other day change a culture solution, culture is to after 14 days, by gel sample
Product, which take out, carries out nestin (Nestin) dyeing.The specific steps of which are as follows: twice with PBS soaking and washing hydrogel sample, being added
4% paraformaldehyde covers cell, and room temperature is fixed again with PBS soaking and washing three times after 15 minutes, and 0.1%Triton X-100 is saturating
Change 5 minutes, lowlenthal serum be added after cleaning and closes 30 minutes, primary antibody is added after removing liquid and cleaning and is placed in 4 DEG C of overnight incubations,
Fluorescence secondary antibody is added in the abundant soaking and washing of PBS five times, and 37 DEG C are protected from light incubation 1h, and DAPI is added in the abundant soaking and washing of PBS three times
Dyeing liquor covers cell, and room temperature dyes 10 minutes, and with PBS infiltration is added after the abundant soaking and washing of PBS three times, copolymerization is burnt micro-
Microscopy surveys observation.As shown in figure 9, cell after dyeing, shows strong green fluorescence, show dry thin on combine dressing
Born of the same parents, the expression of nestin height, mesenchymal stem cell are in Neural Differentiation trend.
Embodiment 5: a kind of piezoelectricity-Signa Gel promoting peripheral nerve reparation and the combine dressing for loading Chinese medicine excretion body
And preparation method thereof, comprising the following steps:
1) by gelatin and methacrylic acid anhydride reactant, room temperature is dialysed 2 weeks in the bag filter of 8k-14kDa later, is then frozen
It is dry, it is prepared into methacrylated gelatin；
2) first west safflower is cleaned, 150 meshes are crossed in juicing；Again by differential centrifugation, successively with 3000g, 5000g from
After the heart, takes supernatant to be centrifuged at 100000g with ultracentrifuge, then precipitating is resuspended with ultrapure water.Use saccharose gradient
Then solution surpassed at 100000g from 60 minutes；The excretion body for taking different component again is centrifuged 60 minutes at 10000g, preparation
At Chinese medicine excretion body；
3) pass through HAuCl4With CTAB, NaBH4It is prepared into seed solution, HAuCl4、CTAB、AgNO3, the Dutch treatment is for Cheng Shengchang
Solution, then by seed mediated growth method, in 25 DEG C, growth overnight, is prepared into gold nanorods
4) its concentration is made to reach 15% the dissolution of methacrylated gelatin ultrapure water；Then gold nanorods are added,
Its concentration is set to reach 2mg/ml；Chinese medicine excretion body is added, its ultimate density is made to reach 0.1 μ g/ml.It is blended into photosensitizer LAP,
Obtain glue pregel system；
5) Kynoar powder monomer is taken, after being dissolved with anhydrous DMF, then rotary coating is annealed for 120 DEG C at film,
After annealing, 60 DEG C, under 120V/ μm of electric field, polarize 8h, Kynoar is prepared, with a thickness of 40 μm；
6) it by hydrogel pregel system, is spun on Kynoar, then photo-crosslinking 80s, is prepared into combine dressing,
Wherein hydrogel thickness is 3mm；
It establishes acute injury model: 48 cleaning grade SD male rats (weight 150-200g) being divided into 8 groups: blank control
Group, OMV solution group, SDF-1 protein solution group, OMV+SDF-1 mixed solution group, Gelma gel group, Gelma/SDF-1 gel
Group, Gelma/OMV gel group, Gelma/OMV/SDF-1 gel group.Every group 6, back hair is cut off after anesthesia, removes 1.5cm
The skin of × 1.5cm, and carried out disinfection with the tincture of iodine.Start to be administered within second day, be administered once every three days.Blank group every is every time
Give 200 μ L PBS, same every of three groups of solution groups 200 μ L solution of administration every time, gel group every every time administration 1.5cm ×
The gel film layer formulation of 1.5cm, and be fixed with sterile patch.And pay attention to observing the formation of the healing state and scar of the surface of a wound
Situation, every 3 days progress once photo takings, and the weight of wound area and rat is measured, calculate healing rate and changes of weight situation.
As shown in FIG. 10 and 11, the weight of composite auxiliary material group is obviously increased compared with blank control group with healing rate, wherein
6th day is in significant difference.Wherein the objective proof combine dressing of weight result and is cured to SD rat without harmful systemic effects
Obviously increasing for conjunction rate proves that compound Fu Liao Eng effectively improves the healing rate of wound.
Histotomy is placed in the reparation box for filling with EDTA antigen retrieval buffer (PH8.0) and carries out antigen retrieval, dripped
Add 3%BSA uniform fold tissue, room temperature is closed 30 minutes, and confining liquid is gently got rid of, and PBS is added dropwise by a certain percentage on slice
Neurofilament protein (NF-L) primary antibody prepared, slice lie against 4 DEG C of overnight incubations in wet box.Slide is placed in PBS to be shaken in decoloration
Washing 3 times, every time 5 minutes is shaken on bed.The secondary antibody that kind corresponding to primary antibody is added dropwise covers tissue, is protected from light 50 points of incubation at room temperature
Clock.After PBS sufficiently rinses 3 times, DAPI dye liquor is added dropwise, is protected from light incubation at room temperature 10 minutes.With after the abundant soaking and washing of PBS five times plus
Enter PBS infiltration, slice is observed under Laser Scanning Confocal Microscope and acquires image.As shown in figure 12, combine dressing group skin histology is cut
In piece, the expression of red fluorescence height, and at filiform, it is seen that the distribution of a large amount of neurofilament.Prove combine dressing can in vivo,
The differentiation of wound nerve is effectively promoted, peripheral nerve injury is repaired.
Claims (10)
1. a kind of preparation method for the Piezoelectric anisotropy dressing for promoting peripheral nerve reparation and wound healing to merge load Chinese medicine excretion body,
Characterized by comprising the following steps:
A. gelatin and methacrylic acid anhydride reactant are prepared into methacrylated gelatin；
B. it by differential centrifugation, density-gradient centrifugation method, prepares and purifies Chinese medicine excretion body；
C. methacrylated gelatin is dissolved in ultrapure water or phosphate buffered saline solution, then by gold nanorods and preparation
Chinese medicine excretion body and methacrylated it is gelatin-compounded, and adulterate photosensitizer, be prepared into conductive pregel system；
D. conductive pregel is spun on poly meta fluoroethylene piezoelectric film, then by photo-crosslinking, is prepared into combine dressing.
2. a kind of piezoelectricity for promoting peripheral nerve reparation and wound healing merging load Chinese medicine excretion body according to claim 1
The preparation method of combine dressing, it is characterised in that: in the step A, the grafting rate of methacrylated gelatin is 20-
90%.
3. a kind of piezoelectricity for promoting peripheral nerve reparation and wound healing merging load Chinese medicine excretion body according to claim 1
The preparation method of combine dressing, it is characterised in that: in the step B, the source medicinal material of excretion body be ginseng, the fruit of glossy privet, Radix Salviae Miltiorrhizae,
One of glutinous rehmannia, west safflower, Chinese herbaceous peony are a variety of.
4. a kind of piezoelectricity for promoting peripheral nerve reparation and wound healing merging load Chinese medicine excretion body according to claim 1
The preparation method of combine dressing, it is characterised in that: the step B specifically:
1) medicinal material cleaned, then squeezed the juice；The juice squeezed crosses the sieve of 100-300 mesh, removes medicinal material residue；By the juice of sieving
Liquid carries out differential centrifugation, first in the case where centrifugal force is 1000-10000g, is centrifuged 10-60 minutes, discards precipitating；Then by supernatant
In the case where centrifugal force is 3000-30000g, it is centrifuged 10-120 minutes；Discard precipitating, by supernatant again centrifugal force be 10000-
Under 300000g, it is centrifuged 10-480 minutes；
2) precipitating for obtaining step 1) final step is resuspended with ultrapure water or phosphate buffered saline solution, then passes through density
Gradient centrifugation purification, density gradient solution are prepared by sucrose or CsCl；Centrifugal force 100000-200000g, time 10-360
Minute；
3) the excretion body in various concentration section is sucked out, is centrifuged at centrifugal force 50000-300000g, the time is 10-360 points
Clock is precipitated.It is resuspended with ultrapure water or phosphate buffered saline solution, obtains Chinese medicine excretion body.
5. a kind of piezoelectricity for promoting peripheral nerve reparation and wound healing merging load Chinese medicine excretion body according to claim 1
The preparation method of combine dressing, it is characterised in that: the draw ratio of the gold nanorods is 1-5.
6. a kind of piezoelectricity for promoting peripheral nerve reparation and wound healing merging load Chinese medicine excretion body according to claim 1
The preparation method of combine dressing, it is characterised in that: in the conductive pregel system of the step C, methacrylated gelatin
Mass percent concentration be 5%-30%；The concentration of nanometer rods is 0.1-5mg/ml；The concentration of Chinese medicine excretion body is 1ng-
10ug/ml；Photosensitizer is one or both of LAP and I2959.
7. a kind of piezoelectricity for promoting peripheral nerve reparation and wound healing merging load Chinese medicine excretion body according to claim 1
The preparation method of combine dressing, it is characterised in that: the poly meta fluoroethylene piezoelectric film the preparation method comprises the following steps: passing through rotary coating
Method coating, is then annealed, polarizing is prepared into poly meta fluoroethylene piezoelectric film；Spin coating rear film with a thickness of 10-50 μm, move back
For the temperature of fire between 60-150 DEG C, poling temperature is 50-150 DEG C, and voltage is 80-200V/ μm, and the polarization time is 1-8 hours.
8. a kind of piezoelectricity for promoting peripheral nerve reparation and wound healing merging load Chinese medicine excretion body according to claim 1
The preparation method of combine dressing, it is characterised in that: in the step D, in conductive pregel system, the photo-crosslinking time is 10-360
Second, methacrylated gelatin is after spin coating with a thickness of 1-5mm.
9. combine dressing made from a kind of -8 any the methods according to claim 1.
10. application of the combine dressing as claimed in claim 9 in intelligent response material, wound healing and neural restoration field.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949677A (en) * | 2018-07-05 | 2018-12-07 | 浙江大学 | Martynoside C and salviandic acid A are promoting Marrow Mesenchymal Stem Cells In Vitro proliferation and are inhibiting the application in replicative senescence |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1424396A (en) * | 2002-12-13 | 2003-06-18 | 中山大学 | Preparation of implanting neurons for treating nerve injury diseases |
| CN103479682A (en) * | 2012-06-14 | 2014-01-01 | 苏州恒宇生物科技有限公司 | Preparation method for plant source active component nano-scale membrane type vesicle |
| CN107802878A (en) * | 2017-09-06 | 2018-03-16 | 华南理工大学 | A kind of modified gelatin/potassium-sodium niobate compound electroactive antibacterial biological dressing and its preparation and application |
| CN107929805A (en) * | 2018-01-10 | 2018-04-20 | 四川大学 | A kind of metal/hydrogel combine dressing for promoting wound healing and preparation method thereof |
| CN108187130A (en) * | 2017-09-15 | 2018-06-22 | 浙江大学 | A kind of reagent and its application for being used for biological damage reparation or hemostasis |
-
2019
- 2019-09-25 CN CN201910910621.6A patent/CN110464867B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1424396A (en) * | 2002-12-13 | 2003-06-18 | 中山大学 | Preparation of implanting neurons for treating nerve injury diseases |
| CN103479682A (en) * | 2012-06-14 | 2014-01-01 | 苏州恒宇生物科技有限公司 | Preparation method for plant source active component nano-scale membrane type vesicle |
| CN107802878A (en) * | 2017-09-06 | 2018-03-16 | 华南理工大学 | A kind of modified gelatin/potassium-sodium niobate compound electroactive antibacterial biological dressing and its preparation and application |
| CN108187130A (en) * | 2017-09-15 | 2018-06-22 | 浙江大学 | A kind of reagent and its application for being used for biological damage reparation or hemostasis |
| CN107929805A (en) * | 2018-01-10 | 2018-04-20 | 四川大学 | A kind of metal/hydrogel combine dressing for promoting wound healing and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王飘飘: "外泌体的提取方法及其在药物递送系统中的应用", 《中国药理学通报》 * |
| 车冠华: "中药诱导骨髓间充质干细胞分化为神经样细胞的研究进展", 《当代医学》 * |
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