CN110447603B - Small insect self-separating counting device without damage to living body - Google Patents
Small insect self-separating counting device without damage to living body Download PDFInfo
- Publication number
- CN110447603B CN110447603B CN201910839749.8A CN201910839749A CN110447603B CN 110447603 B CN110447603 B CN 110447603B CN 201910839749 A CN201910839749 A CN 201910839749A CN 110447603 B CN110447603 B CN 110447603B
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- chamber
- separation chamber
- dilution separation
- counting
- primary
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- 241000238631 Hexapoda Species 0.000 title claims abstract description 75
- 238000000926 separation method Methods 0.000 claims abstract description 87
- 238000010790 dilution Methods 0.000 claims abstract description 86
- 239000012895 dilution Substances 0.000 claims abstract description 86
- 238000007789 sealing Methods 0.000 claims abstract description 16
- 238000013508 migration Methods 0.000 claims abstract description 13
- 230000005012 migration Effects 0.000 claims abstract description 13
- 238000012360 testing method Methods 0.000 claims abstract description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 8
- 238000003780 insertion Methods 0.000 claims description 7
- 230000037431 insertion Effects 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 4
- 238000011160 research Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 238000007865 diluting Methods 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 230000006399 behavior Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06M—COUNTING MECHANISMS; COUNTING OF OBJECTS NOT OTHERWISE PROVIDED FOR
- G06M11/00—Counting of objects distributed at random, e.g. on a surface
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Theoretical Computer Science (AREA)
- Catching Or Destruction (AREA)
Abstract
The living body harmless small insect self-separating counting device comprises an insect collecting chamber, a primary dilution separation chamber, a secondary dilution separation chamber and a counting chamber which are connected in sequence, wherein a shading black sleeve cover is covered outside the insect collecting chamber, and flashboards are respectively arranged between the insect collecting chamber and the primary dilution separation chamber, between the primary dilution separation chamber and the secondary dilution separation chamber and between the secondary dilution separation chamber and the counting chamber; the top of the primary dilution separation chamber and the top of the secondary dilution separation chamber are respectively provided with a migration aid, the migration aids are respectively sealed by an escape-proof sleeve cover, the insect collecting chamber is provided with a movably-installed sealing plate, the counting chamber is a replaceable transparent test tube, and the insect collecting chamber is externally sleeved with a shading black sleeve cover. The invention has simple structure, convenient operation and portability, and can realize automatic lossless dilution separation and accurate counting of small insects.
Description
Technical Field
The invention relates to the technical field of self-separating counting of small insects, in particular to a device for automatically separating and accurately counting living small insects with overhigh experimental population density and high activity speed.
Background
In the current research fields of insect behaviours and pest insect control, the research content of the research is always to accurately count, transfer, propagate and the like of insects, none of the researches is based on an accurate insect counting method, so that the accurate, rapid and harmless counting of living small insects is an urgent problem to be solved in the experimental research of the insect behaviours.
Today, few methods for separating and counting living small insects with high population density and high activity speed are available and the accuracy is not high. The existing separation method is harmful to the integrity of insects, thereby affecting the physiological and biochemical processes and the life and development of insects, and a device capable of reducing manpower consumption and accurately counting living insects is urgently needed.
Disclosure of Invention
The invention aims to provide an experimental device capable of accurately counting living insects without damage while reducing manpower consumption, and has the effect of enabling living small insects to self-separate and accurately count. Solves the problem of the necessary precise and damage-free insect counting method and technique in the research field of insect behaviours and the prevention and treatment of harmful insects, and provides a counting device for accurately, rapidly and nondestructively counting living small insects.
To achieve the above object, embodiments of the present invention are as follows: the self-separating counting device for the living intact small insects comprises an insect collecting chamber, a primary dilution separation chamber, a secondary dilution separation chamber and a counting chamber which are connected in sequence; gate plates are respectively arranged between the insect collecting chamber and the primary dilution separation chamber, between the primary dilution separation chamber and the secondary dilution separation chamber, and between the secondary dilution separation chamber and the counting chamber; the tops of the primary dilution separation chamber and the secondary dilution separation chamber are respectively provided with an insertion hole, the auxiliary moving device is respectively inserted into the primary dilution separation chamber and the secondary dilution separation chamber through the insertion holes, and the auxiliary moving device is respectively sealed through an anti-escape sleeve cover; the pest collecting chamber is provided with a movably mounted front sealing plate, the counting chamber is a replaceable transparent test tube, and the pest collecting chamber is externally sleeved with a shading black sleeve cover.
As optimization, the flashboard between the insect collecting chamber and the primary dilution separation chamber, between the primary dilution separation chamber and the secondary dilution separation chamber and between the secondary dilution separation chamber and the counting chamber are respectively arranged between the insect collecting chamber and the primary dilution separation chamber, between the primary dilution separation chamber and the secondary dilution separation chamber and between the counting chamber and the secondary dilution separation chamber through clamping grooves, and the upper end of each flashboard is provided with a handle.
As optimization, the front sealing plate is arranged at the rear end of the insect collecting chamber through a clamping groove, and a handle is arranged on the front sealing plate.
As optimization, the lower section of the migration aid is a soft biological hair brush or a synthetic brush, the escape-proof sleeve cover is a cone sleeve cover made of fine spun yarns, the upper end of the migration aid is embedded with a telescopic rubber band, and the lower end of the migration aid is adhered to the inner diameter of an insertion hole in a seamless manner.
As optimization, a fixed table is arranged below the counting chamber, and the bottom of the counting chamber and the bottom of the secondary dilution separation chamber are kept horizontal.
As optimization, the chamber body formed by the insect collecting chamber, the primary dilution separation chamber and the secondary dilution separation chamber and the front sealing plate are made of transparent organic glass.
The technical scheme of the invention has the following advantages:
1. the technology solves the problem of damage-free accurate counting of living small insects with high population density and high moving speed, and provides relevant necessary basic methods and technologies for insect life form research, insect behavioural research and pest control research;
2. the shading black cover can isolate external light to form a darkroom, and the phototaxis of insects are utilized to promote the insects to move towards the diluting and separating chamber so as to achieve the purpose of gradual self-diluting and separating, so that the insects are not damaged, and accurate counting is convenient;
3. the primary dilution separation chamber and the secondary dilution separation chamber of the invention have the functions of gradually reducing the density of insects, diluting and autonomously separating insects, and can flexibly move up and down through the flashboard as required, and open (pull up) or close (put down) each functional chamber at any time, so that a passage is opened or closed for the insects to move into the counting chamber, the operation is simple and convenient, and the accurate counting is convenient.
4. The migration aid is a soft biological hair brush or a synthetic brush, ensures that insect bodies are not damaged, keeps the integrity of the insect bodies, can freely move in a certain space through the escape-proof sleeve cover, and plays a role in assisting insects to enter the next chamber.
6. The counting chamber is a replaceable transparent test tube, is convenient to count and collect, and is convenient to study. And the counting chamber is fixed by the fixer, so that the counting chamber is stable, and meanwhile, the counting chamber and the channel 3 of the secondary dilution separation chamber are in the same horizontal position and are not sleeved. Ensuring that the insects to be separated enter the counting chamber according to the density requirements.
In a word, the self-separating counting device for the small insects with no damage to living bodies has the advantages of simple structure, convenient operation, easy operation, accurate counting and convenient carrying, and provides a method and a technology for automatic and damage-free dilution separation and accurate counting of the small insects.
Drawings
FIG. 1 is a schematic view of the self-contained structure of the present invention
FIG. 2 is a schematic view of the structure of the black mask of the present invention
In the figure: 1-insect collecting chamber, 11-front sealing plate 1, 12-handle and 13-handle hole; 14-clamping grooves; 15-insects to be separated and counted, 16-flashboard 1, 17-clamping groove 2, 18-handle, 19-handle hole, 110-channel and 111-shading black cover; 2-primary dilution separation chamber, 21-auxiliary moving device, 22-escape-proof sleeve cover, 23-inserting hole, 24-flashboard 2, 25-handle, 26-handle hole, 27-clamping groove, 28-channel, 3-secondary dilution separation chamber, 31-auxiliary moving device, 32-escape-proof sleeve cover, 33-inserting hole, 34-back closing plate, 35-flashboard, 36-handle, 37-handle hole, 38-clamping groove, 39-channel, 4-counting chamber, 41-fixed table and 5-chamber body.
Detailed Description
In order to more clearly illustrate the technical scheme of the invention, the invention is described in detail below with reference to the accompanying drawings. It should be noted that, the present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the protection scope of the present invention is not limited thereto.
As shown in fig. 1 and 2, the self-separating counting device for the small insects with no living damage comprises a chamber body 5 and a counting chamber 4 which are made of transparent organic glass materials, wherein the chamber body 5 consists of a pest collecting chamber 1, a primary dilution separation chamber 2 and a secondary dilution separation chamber 3, and a shading black cover 111 is arranged on the outer cover of the pest collecting chamber 1. The pest-collecting chamber 1 is connected with the primary dilution separation chamber 2, the secondary dilution separation chamber 3 is connected with the primary dilution separation chamber 2, and the counting chamber 4 is connected with the secondary dilution separation chamber 3. The counting chamber 4 is a replaceable transparent test tube, is seamlessly sleeved on the lower part of the rear sealing plate 34 of the secondary dilution separation chamber 3, and is provided with a fixing table 41 below the counting chamber 4 for fixing the counting chamber 4 and ensuring that the counting chamber 4 is parallel to the bottom of the secondary dilution separation chamber 3.
As shown in fig. 1 and 2, shutters 16, 24, and 35 are provided between the insect collecting chamber 1 and the primary dilution separation chamber 2, between the primary dilution separation chamber 2 and the secondary dilution separation chamber 3, and between the secondary dilution separation chamber 3 and the counting chamber 4, respectively; u-shaped clamping grooves 17, 27 and 38 made of rubber materials are symmetrically arranged on the inner walls of the two sides between the insect collecting chamber 1 and the primary dilution separation chamber 2, between the primary dilution separation chamber 2 and the secondary dilution separation chamber 3 and between the secondary dilution separation chamber 3 and the counting chamber 4, the flashboard 16, 24 and 35 are respectively sleeved in the clamping grooves 17, 27 and 38, and handles 18, 25 and 36 are arranged at the upper ends of the flashboard 16, 24 and 35. The top of the primary dilution separation chamber 2 and the secondary dilution separation chamber 3 are respectively provided with insertion holes 23 and 33, respectively, the migration aids 21 and 31 are respectively inserted into the primary dilution separation chamber 2 and the secondary dilution separation chamber 3 through the insertion holes 23 and 33, and the migration aids 21 and 31 are respectively sealed by the escape-preventing covers 22 and 32. The lower sections of the migration aids 21 and 31 are soft biological hair brushes or artificial synthetic brushes, the escape-preventing sleeve covers 22 and 32 are cone sleeve covers made of fine spun yarns, the upper ends of the escape-preventing sleeve covers are embedded with telescopic rubber bands, and the lower ends of the escape-preventing sleeve covers are adhered to the inner diameters of the insertion holes 23 and 33 in a seamless mode.
As shown in fig. 1 and 2, a front sealing plate 11 made of transparent organic glass is arranged at one end of the insect collecting chamber 1 opposite to the primary dilution separation chamber 2, the front sealing plate 11 is sleeved in a U-shaped clamping groove 14 made of rubber, and a handle 12 is arranged at the upper end of the front sealing plate 11. The front sealing plate 11 can be pulled up and down through the handle 12, and the front sealing plate 11 is put down to form the insect collecting chamber 1.
The operation method of the living body harmless small insect self-separating counting chamber is as follows:
(1) The closing plate 11 is pulled upwards through the handle 12, after the insects with high density to be separated are put into the container, the closing plate 11 is put down, and the shading black cover 111 is sleeved, so that the insect collecting chamber 1 with the insects with high density is formed;
(2) The flashboard 16 between the insect collecting chamber 1 and the primary dilution separation chamber 2 is pulled up through the lifting handle 18 to form a channel 110, so that insects in the insect collecting chamber 1 enter the primary dilution separation chamber 2 through the channel 110 in sequence, and the insects can be pushed to move towards the secondary dilution separation chamber 3 by the aid of the migration aid 21;
(3) The flashboard 24 between the primary dilution separation chamber 2 and the secondary dilution separation chamber 3 is pulled up through the lifting handle 25 to form a channel 28, so that insects in the primary dilution separation chamber 2 enter the secondary dilution separation chamber 3 through the channel 28 in sequence, and the movement of the insects towards the counting chamber 4 can be assisted by the aid of the movement aid 31;
(4) The shutter 35 between the secondary diluting and separating chamber 3 and the counting chamber 4 is pulled up by the handle 36, the passage 39 is exposed, and insects in the secondary diluting and separating chamber 3 enter the counting chamber 4 through the passage 39, so that the number of insects entering the counting chamber 4 is accurately counted.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications in form, such as the number and grade of dilution separation chambers, will be apparent to persons skilled in the art from the foregoing description. It is not necessary here nor is it exhaustive of all embodiments. While obvious variations or modifications are contemplated as falling within the scope of the present invention.
Claims (3)
1. The self-separating counting device for the living intact small insects is characterized by comprising an insect collecting chamber (1), a primary dilution separation chamber (2), a secondary dilution separation chamber (3) and a counting chamber (4) which are connected in sequence, wherein flashboards (16, 24, 35) are respectively arranged between the insect collecting chamber (1) and the primary dilution separation chamber (2), between the primary dilution separation chamber (2) and the secondary dilution separation chamber (3) and between the secondary dilution separation chamber (3) and the counting chamber (4); the tops of the primary dilution separation chamber (2) and the secondary dilution separation chamber (3) are respectively provided with an inserting hole (23, 33), respectively facilitate the migration aid (21, 31) to be inserted into the primary dilution separation chamber (2) and the secondary dilution separation chamber (3) through the inserting holes (23, 33), and respectively seal the migration aid (21, 31) through the escape-preventing cover (22, 32); the pest collecting chamber (1) is provided with a front sealing plate (11) which is movably arranged, the counting chamber (4) is a replaceable transparent test tube, and the pest collecting chamber (1) is sleeved with a shading black sleeve cover (111); the shutters (16, 24, 35) between the insect collecting chamber (1) and the primary dilution separation chamber (2), between the primary dilution separation chamber (2) and the secondary dilution separation chamber (3) and between the secondary dilution separation chamber (3) and the counting chamber (4) are respectively arranged between the insect collecting chamber (1) and the primary dilution separation chamber (2), between the primary dilution separation chamber (2) and the secondary dilution separation chamber (3) and between the secondary dilution separation chamber (3) and the counting chamber (4) through clamping grooves (17, 27, 38), and handles (18, 25, 36) are arranged at the upper ends of the shutters (16, 24, 35); the front sealing plate (11) is arranged at the rear end of the insect collecting chamber (1) through a clamping groove (14), and a handle (12) is arranged on the front sealing plate (11); the lower section of the migration aid (21, 31) is a soft biological hair brush or a synthetic brush, the escape-proof sleeve cover (22, 32) is a cone sleeve cover made of fine spun yarns, the upper end of the escape-proof sleeve cover is embedded with a telescopic rubber band, and the lower end of the escape-proof sleeve cover is adhered to the inner diameter of the insertion hole (23, 33) in a seamless mode.
2. The living intact small insect self-separating and counting device according to claim 1, characterized in that a fixed table (41) is arranged under the counting chamber (4) to keep the bottom of the counting chamber (4) and the secondary dilution separation chamber (3) horizontal.
3. The living intact small insect self-separating and counting device according to claim 1, wherein the chamber body (5) consisting of the insect collecting chamber (1), the primary dilution and separation chamber (2) and the secondary dilution and separation chamber (3) and the front sealing plate (11) are made of transparent organic glass.
Priority Applications (1)
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CN201910839749.8A CN110447603B (en) | 2019-09-06 | 2019-09-06 | Small insect self-separating counting device without damage to living body |
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CN201910839749.8A CN110447603B (en) | 2019-09-06 | 2019-09-06 | Small insect self-separating counting device without damage to living body |
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CN110447603A CN110447603A (en) | 2019-11-15 |
CN110447603B true CN110447603B (en) | 2024-02-09 |
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