CN110447471A - Plant cultivation device is used in a kind of experiment of blade inoculation endophyte of plant - Google Patents

Abstract

A plant culture device for leaf inoculation plant endophytic bacteria experiments, comprising a cylindrical device body, the upper part of the device body is a cover, the middle of the cover is provided with a bottle mouth, and the bottle mouth is provided with a bacteria-filtering air-permeable membrane. The upper part of the cover is provided with an opening, and the opening is provided with a telescopic device, and the end of the telescopic device is connected to the nozzle. The nozzle is provided at one end of the nozzle located inside the device body, and a pressure device is provided at the end located outside the device body. and extended hoses. The culture device of the present invention can well adjust the spraying position of the nozzle at the end of the nozzle in the culture bottle through the design of the opening, so as to realize the precise spraying and inoculation of the leaves of the cultured plants; the aseptic state in the culture bottle can be maintained, and the maximum Reduce the impact of other microorganisms on the biological effects of endophytes on the experimental host plant, reflecting the real role of endophytes.

Description

A plant culture device for leaf inoculation plant endophyte experiment

technical field

The invention relates to the technical field of experimental devices, in particular to a plant culture device for the experiment of leaf inoculation with plant endophytes.

Background technique

Plant endophytes are fungi or bacteria that live in the tissues and organs of healthy plants for some or all stages. Plant endophytes are fungi that can not only produce various chemical substances, but also inhibit or kill various pathogenic bacteria through competition or other effects, and do not cause obvious disease symptoms to plant tissues, including those in the life cycle. Saprophytic bacteria that grow on the surface of the camp at a certain stage, latent pathogenic bacteria and mycorrhizal fungi that are temporarily harmless to the host, etc., can be isolated from several to dozens of endophytic bacteria on one plant. Plant endophytes are a new microbial resource with great potential application value.

The traditional view is that chemical control is the most effective way to control crop diseases, but chemical fungicides pollute the environment, induce increased resistance of pathogens, destroy ecological balance, and cause residual pollution. Therefore, research on biological control of plant diseases has attracted more and more attention. In the research, people found that there are a large number of beneficial endophytic bacteria and endophytic fungi in plants. The biological control and development and application of these endophytic bacteria on plant diseases have attracted widespread attention and attention from scientists. Plant pathogenic antagonists with the same biological control effect are usually isolated and screened from plant rhizosphere soil. They are soil microorganisms that are easily affected by external conditions. Their survival needs to compete with soil habitant microorganisms and are usually not easy to dominate for a long time, and it is difficult for pathogenic bacteria to survive. It plays a practical role in the living environment, which greatly affects its actual disease prevention effect. Different from phytopathogenic antagonistic bacteria, plant endophytic bacteria can colonize in plants, and the colonized bacteria are protected by plants and less affected by external conditions. They can play biological roles for a long time and have more utilization value; Similar to phytopathogenic antagonistic bacteria, endophytes are also affected by various biotic and abiotic factors during the colonization process. The interaction between endophytes and host plants will affect the colonization of endophytes in vivo. It is often necessary to rule out the influence of other factors.

The method of artificial inoculation can inoculate endophytes with disease-preventing activity or other functions into plants, and play the role of endophytes in preventing diseases and insect pests. important means. The methods of artificially inoculating endophytic bacteria commonly used at present have following 3 kinds: 1. root irrigation inoculation method, irrigate plant root with endophyte suspension, endophyte can be made to enter in the plant rhizome; 2. soaking inoculation method, before sowing The seeds of the host plant are soaked in the suspension containing endophytes for a certain period of time, and the marked endophytes can be detected in the plants grown from suitable host plants; The suspension of endophytes is sprayed on the leaves of host plants to allow the endophytes to colonize in suitable host plants. In the process of artificially inoculating endophytic bacteria, in order to avoid the influence of other microorganisms on the experimental endophytic inoculation, it needs to be carried out in a sterile culture environment. The current plant culture device usually has a cover with a filter and a breathable membrane on the top These devices are feasible for the above two inoculation methods ① and ②, especially the ② inoculation method only needs to place the treated seeds in the cultivation device at one time, and control the temperature externally. There is no need to open the culture device during the process of waiting for the culture conditions until the end of the experiment, so as to maintain the consistency of the culture environment well. However, the inoculation method in the above-mentioned step ③ needs to be sprayed and inoculated after the leaves grow, and the existing culture device needs to be opened. The upper cover is manually sprayed, so it is difficult to maintain the consistency of the culture environment in the existing culture device, and the experiment process is easily affected by other microorganisms. At present, there are few reports on the special culture device used in this experiment, but this culture device is a device with cutting-edge experimental significance.

Contents of the invention

Aiming at the deficiencies of the prior art, the invention provides a special plant culture device for the experiment of leaf inoculation with plant endophytes.

The technical solution adopted by the present invention to solve the problems of the technologies described above is:

A plant culture device for leaf inoculation plant endophytic bacteria experiment, comprising a cylindrical device body, the upper part of the device body is a cover, the middle of the cover is provided with a bottle mouth, and the bottle mouth is provided with a bacteria-filtering air-permeable membrane. The upper part of the cover is provided with an opening, and the opening is provided with a retractable device. The end of the retractable device is connected to the nozzle. and extended hoses.

Further, the mouth of the bottle is a circular opening protruding to the outside of the cover, and the bacteria-filtering gas-permeable membrane is sleeved on the outside of the circular opening through an elastic ring, and the elastic ring is a seal with certain elasticity. rings or ordinary elastic hoops.

Further, the telescopic device is a soft sleeve, the upper end of which is in sealing connection with the opening, and the lower end is in sealing connection with the outer edge at a certain position between the two ends of the nozzle, and the sealing connection is preferably Adhesive connection, the above-mentioned certain position is preferably in the middle or upper third of the nozzle, the soft sleeve can be stretched into the device body from the opening of the device body through the pulling of the nozzle, or the nozzle can be pulled The pull extends out of the device body from the device body opening.

Further, the spray pipe is a hard hollow pipe, one end inside the device body is provided with a spray head, and one end outside the device body is provided with a pressurizing device and an extended hose.

Further, a thermometer is arranged inside the device body.

Further, the device body is a transparent glass bottle or a transparent plastic bottle.

Compared with the prior art, the plant cultivation device of the present invention has the following advantages:

1. It can be reused after cleaning and sterilization;

2. It is easy to spray and inoculate plant leaves, because the position of the growing leaves of the plants in the culture bottle has no regularity, and the culture device of the present invention can well adjust the position of the spray nozzle at the end of the nozzle in the culture bottle by the design of the opening. Spraying position to achieve precise spraying and inoculation of cultivated plant leaves;

3. It can maintain the aseptic state in the culture bottle, minimize the influence of other microorganisms on the biological effects of endophytes on the experimental host plants, and reflect the true role of endophytes.

Description of drawings

Fig. 1 is a structural representation of the present invention;

Fig. 2 is another structural schematic diagram of the present invention.

Detailed ways

The present invention will be further described below in conjunction with the accompanying drawings.

A kind of plant cultivation device for leaf inoculation plant endophytic bacteria experiment as shown in Figure 1, 2, comprises cylindrical device body 1, and described device body 1 top is cover body 2, and cover body 2 and device body 1 can be sealed connection; the middle of the cover body 2 is provided with a bottle mouth 21, and the open bottle mouth 21 is provided with a bacteria-filtering breathable membrane 3, and the upper part of the cover body 2 is provided with an opening 22, and the opening 22 is provided with a retractable device 4, which can be stretched The end 41 of the device 4 is connected to the nozzle 5 , and the nozzle 51 is provided at one end of the nozzle 5 inside the device body 1 , and a pressurizing device 52 and an outwardly extending hose 53 are provided at one end outside the device body 1 .

Further, the bottle mouth 21 is a circular opening protruding to the outside of the cover body 2, and the bacteria-filtering gas-permeable membrane 3 is sleeved on the outside of the circular opening through an elastic ring, and the elastic ring has a certain Elastic sealing ring or ordinary elastic hoop.

Further, the telescopic device 4 is a soft folded telescopic sleeve, the upper end 42 of which is in sealing connection with the opening 22, and the lower end 41 is in a sealing connection with the outer edge at a certain position between the two ends of the nozzle 5. , the sealed connection is preferably an adhesive connection, and a certain position is preferably in the middle or upper third of the nozzle 5, and the soft folded sleeve can be drawn from the opening 22 of the device body by pulling the nozzle 5 Extend into the inside of the device body 1, as shown in Figure 1; or extend out of the device body 1 from the opening 22 of the device body through the pulling of the nozzle 5, as shown in Figure 2; the soft folded sleeve is preferably soft and transparent The plastic pleated sleeve is used to reduce the shading effect of the retractable device 4 on the interior of the device body 1; the lower end 41 of the soft pleated sleeve is fixed and sealed with the nozzle 5, and the nozzle 5 can pass through the soft pleats through external force. The sealing connection at the lower end of the sleeve is drawn outwards, inwards and rotated.

Further, the nozzle pipe 5 is a hard hollow pipe, one end inside the device body 1 is provided with a spray head 51, and one end outside the device body 1 is provided with a pressurizing device 52 and communicated with an extended hose 53 , the end of the extended hose 53 is connected to the endophyte liquid storage container 7, the endophyte liquid storage container 7 is filled with the endophyte liquid, and the endophyte liquid is manually pressurized by the pressurizing device 52 Spray on the blade in the cultivating device by shower nozzle 51 after; The spray device that described pressurizing device 52, spray pipe 5 and shower nozzle 51 are formed is the manual spray device commonly used in the prior art, and its structure is as watering can, and is different from watering can What’s more, it adds a spray pipe between the pressurizing device and the nozzle; preferably, the endophytic bacteria liquid storage container 7 is provided with a scale for recording the amount of inoculated and sprayed endophytic bacteria liquid, spraying endophytic bacteria liquid The recording method of the amount of bacteria liquid is: scale 1 when the nozzle 51 starts to discharge, and scale 2 when the inoculation and spraying is completed. Quantity; Preferably, the plant endophyte bacterial liquid is the plant endophyte suspension after purification, isolation and screening.

Further, a thermometer 6 is arranged inside the device body 1 .

Further, the device body 1 is a transparent glass bottle or a transparent plastic bottle, and in other embodiments of the present invention, the bottle body made of other transparent materials can also be selected.

Use the cultivation device of the present invention to carry out the reference of the experimental process of leaf inoculation of plant endophytes: after the device and the soil in the device are sterilized by high temperature or irradiation or other means, the seeds for testing are soaked in sterile water and then sowed After being placed in the aseptic soil installed in the device body 1, conventional seedlings are raised at normal temperature. After 2-3 true leaves are grown, the endophytic bacteria liquid is sprayed by the nozzle nozzle 51 to the blades, and the sterile water can be used as the same time. In the control example, the same spraying experiment was carried out on the leaves of other plants to be tested under the same conditions. The experiment of endophyte inoculation is generally one-time inoculation, and in special cases, when needing secondary inoculation, culture device of the present invention can carry out secondary inoculation experiment through nozzle 5, as the endophyte that needs experiment is two kinds of different Endophytic bacteria can be selected to be provided with two identical openings 22, nozzles 5 and other devices at the top of the culture device of the present invention. In addition, as shown in Figure 2, one or more bottle body openings 11 with a bacteria-filtering gas-permeable membrane 3 can be optionally provided on the body 1 of the culture device of the present invention. When multiple bottle body openings 11 are set, the bottle body opening 11 Should be at the same level and evenly distributed on the body 1, the opening 11 of the bottle body is used to increase the air circulation in the bottle.

Many specific details have been set forth in the above description in order to fully understand the present invention, but the present invention can also be implemented in other ways different from the scope of this description, so the scope of protection of the present invention is not limited by the specific embodiments disclosed above. limits.

Claims (6)

1. A plant culture device for leaf inoculation plant endophytic bacteria experiment, characterized in that: it comprises a cylindrical device body, the upper part of the device body is a cover, the middle of the cover is provided with a bottle mouth, and the bottle mouth is provided with a filter. Bacteria gas-permeable membrane, the upper part of the cover is provided with an opening, the opening is provided with a retractable device, the end of the retractable device is connected to the nozzle, the nozzle is located at the end of the device body, and the nozzle is located outside the device body. One end is provided with a pressurizing device and an extended hose. 2. A kind of plant culture device for leaf inoculation plant endophyte experiment according to claim 1, it is characterized in that: described bottle mouth is the circular opening that protrudes to the outside of cover body, and described bacteria filter is breathable The membrane is sheathed on the outside of the circular opening through the elastic ring. 3. A kind of plant culture device for leaf inoculation plant endophyte experiment according to claim 1, it is characterized in that: described telescopic device is soft sleeve pipe, and its upper end is sealed with opening, and lower end is with The outer edge at a certain position between the two ends of the nozzle is tightly connected. 4. The plant culture device for a kind of leaf inoculation plant endophyte experiment according to claim 1 or 3, characterized in that: the nozzle is a hard hollow tube, and one end inside the device body is provided with a As for the spray head, one end outside the device body is provided with a pressurizing device and an extended hose. 5 . The plant culture device for the experiment of leaf inoculation with endophytic bacteria according to claim 1 , wherein the device body is a transparent glass bottle or a transparent plastic bottle. 6 . 6 . The plant culture device for the experiment of leaf inoculation with endophytic bacteria according to claim 1 or 5 , wherein a thermometer is arranged inside the device body. 7 .