CN110447402A - A method of improving laurustinus tip of a root mitosis metaphase phase - Google Patents
A method of improving laurustinus tip of a root mitosis metaphase phase Download PDFInfo
- Publication number
- CN110447402A CN110447402A CN201910805237.XA CN201910805237A CN110447402A CN 110447402 A CN110447402 A CN 110447402A CN 201910805237 A CN201910805237 A CN 201910805237A CN 110447402 A CN110447402 A CN 110447402A
- Authority
- CN
- China
- Prior art keywords
- laurustinus
- root
- tip
- improving
- rooted cuttings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The present invention provides a kind of methods for improving laurustinus tip of a root mitosis metaphase phase, and selection root quantity first is 10-20 item, and root length is that the laurustinus Rooted Cuttings of 5-7cm are stand-by；Then the laurustinus Rooted Cuttings of selection are placed in progress dark processing 36-48h at 14 DEG C -16 DEG C；The laurustinus Rooted Cuttings that dark processing terminates finally are placed in progress lighting process 60min at 24 DEG C -26 DEG C, take the tip of a root that rear microscopy is fixed the laurustinus Rooted Cuttings that lighting process terminates.Operation of the present invention process is simple, practicability is prominent, pass through the culture of high quality Rooted Cuttings, the Combination Design of dark processing and lighting process, obtain clearly metaphase chromosome split coil method, high quality material is provided for karyotyping and FISH analysis, and in all operating process, the harmful chemicals of severe toxicity is not used, so that the risk of experimentation is small, it is environmentally friendly.
Description
Technical field
The invention belongs to phytogenetic studies field, in particular to a kind of raising laurustinus tip of a root mitosis metaphase phase
Method.
Background technique
Laurustinus also known as hydrangea, hydrangea are Saxifragaceae Hydrangea plant.Laurustinus flower pattern is very large, and color is gorgeous
Beautiful, florescence is long, and pattern energy is red can be blue, and leaf is big and has gloss, not only considerable flower but also considerable leaf, in Europe, Dutch, Germany and method
State's Field test is universal.The universal open country plantation in the Yangtze river basin each province in China, the more greenhouse pot cultures in the north.Laurustinus is a kind of both to fit
Suitable garden cultivation, and the ideal flowers and trees for being suitble to potting ornamental, potting can be to arrange exhibition room, the hall.Because its pattern is abundant,
Corolla is big, is rich in rendering power, and decorative strong, silk ball Fresh Cutting flower is got in the application rented in pendulum, wedding, celebration activity and floriculture works
Carry out more popular welcomes by consumer.Currently, the cytology research about laurustinus be concentrated mainly on ploidy,
Research is less in terms of Chromosomes Banding and karyotyping etc., meiotic behavior and Chromosomal in situ hybridization.Karyotyping
The important means of plant classification and genetic research with fluorescence in situ hybridization technique, two kinds of analysis methods all to chromosome separation when
The integrality of phase and chromosome structure has high requirement, needs to obtain suitable division period and the intact dye of chromosome structure
Colour solid film-making, the incomplete chromosome sectioning of and structure few according to division phases, can not obtain ideal chromosome
Observation is as a result, time-consuming take a lot of work again.As it can be seen that the key of chromosome sectioning is to improve the ratio of its mitosis metaphase phase, to obtain
The image for obtaining clearer Metaphase Chromosomes, to obtain ideal experimental material.
Summary of the invention
In order to solve the defects of prior art, the present invention provides a kind of raising laurustinus tip of a root mitosis metaphase phases
Method, comprising the following steps:
Step 1: raw material is chosen, and selection root quantity is 10-20 item, and root length is that the laurustinus Rooted Cuttings of 5-7cm wait for
With；
Step 2: the laurustinus Rooted Cuttings of selection are placed in progress dark processing 36-48h at 14 DEG C -16 DEG C by dark processing；
Step 3: the laurustinus Rooted Cuttings that dark processing terminates are placed at 24 DEG C -26 DEG C and carry out at illumination by lighting process
Manage 60min；
Step 4: the laurustinus Rooted Cuttings that lighting process terminates are taken the tip of a root that rear microscopy is fixed by fixed microscopy.
Further: selected laurustinus Rooted Cuttings are pre-processed by following steps in step 1, first from silk ball
It takes the top tip of 3-5cm long to be cutting and do thin leaf processing on flower maternal plant, is then inserted into flower mud, and it is 20- that temperature, which is arranged,
28 DEG C, air humidity 80-90%, intensity of illumination 7000-15000lux, after thering is root system to grow, finally move it into battalion
It cultivates in nutrient solution wait select.
Further: the light source of lighting process is direct sunlight in step 3.
Further: the light source of lighting process is LED lamplight in step 3.
Further: LED lamplight is specifically that light quantum flux is 60 ± 5 μm of ol/m2/ s, and white light: green light=3:2
LED lamplight.
Further: dark processing temperature is 15 DEG C, and lighting process temperature is 25 DEG C.
Further: the concrete operation step that leaf processing is dredged in cutting is to retain top 2-4 piece leaf, and big blade, which is cut, stays 1/3-
1/2。
It is further: by cutting be inserted into flower mud before, place it in be mixed with 60mg/L methyl α-naphthyl acetate, 60mg/L indolebutyric acid,
5min is impregnated in the treatment fluid of 0.086mg/L Bifenazate and 0.13mg/L pyraclostrobin.
Further: the length, width and height of flower mud are respectively 0.7cm, 0.7cm and 2cm.
Further: nutrient solution is that Japanese garden formula formula nutritional liquid adds 800 times of antibacterial nutrient solutions of earthworm enzyme polypeptide plant.
The utility model has the advantages that operation of the present invention process is simple, practicability is prominent, is set by the combination of dark processing and lighting process
Meter, obtains clearly metaphase chromosome metaphase phase, provides height for the karyotyping and FISH analysis in later period
Quality material, and in all operating process, the harmful chemicals of severe toxicity is not used, so that the risk of experimentation is small,
It is environmentally friendly.
Detailed description of the invention
Fig. 1 is the laurustinus Rooted Cuttings root tip chromosomes mitosis metaphase phase microscopy picture in the embodiment of the present invention.
Specific embodiment
In the following with reference to the drawings and specific embodiments, the present invention is furture elucidated, it should be understood that these embodiments are merely to illustrate
It the present invention rather than limits the scope of the invention, after the present invention has been read, those skilled in the art are to of the invention each
The modification of kind equivalent form falls within the application range as defined in the appended claims.
The method that the present invention improves laurustinus tip of a root mitosis metaphase phase, specifically includes the following steps:
Step 1: raw material is chosen
Selection root quantity is 10-20 item, and root length is stand-by, the selected silk ball of the laurustinus Rooted Cuttings of 5-7cm
Flower Rooted Cuttings are pre-processed by following steps, and the top tip of 3-5cm long is taken to be cutting and retain from laurustinus maternal plant first
Top 2-4 piece leaf, big blade, which is cut, stays 1/3-1/2, then place it in be mixed with 60mg/L methyl α-naphthyl acetate, 60mg/L indolebutyric acid,
5min is impregnated in the treatment fluid of 0.086mg/L Bifenazate and 0.13mg/L pyraclostrobin, then is inserted into flower mud, and
It is 20-28 DEG C, air humidity 80-90%, intensity of illumination 7000-15000lux that temperature, which is arranged, after having root system to grow, most
After move it into nutrient solution and cultivate wait select, nutrient solution is that Japanese garden formula formula nutritional liquid adds 800 times of earthworm enzyme polypeptide plants
Antibacterial nutrient solution；
Step 2: dark processing
The laurustinus Rooted Cuttings of selection are placed at 14 DEG C -16 DEG C, preferably 15 DEG C progress dark processing 36-48h；
Step 3: lighting process
The laurustinus Rooted Cuttings that dark processing terminates are placed at 24 DEG C -26 DEG C, preferably 25 DEG C progress direct sunlights or
LED lamplight lighting process 60min, if selection LED lamplight lighting process, LED lamplight is specifically that light quantum flux is 60
±5μmol/m2/ s, and white light: green light=3:2 LED lamplight；
Step 4: fixed microscopy
Take the tip of a root that rear microscopy is fixed the laurustinus Rooted Cuttings that lighting process terminates.
Embodiment 1
Step 1: raw material is chosen
The top tip of 10 3cm long is taken to be cutting and retain 2 leaves in top from 1 plant of healthy laurustinus maternal plant first, greatly
Blade, which is cut, stays 1/3, is then placed in its whole and is mixed with 60mg/L methyl α-naphthyl acetate, 60mg/L indolebutyric acid, 0.086mg/L biphenyl hydrazine
5min is impregnated in the treatment fluid of ester and 0.13mg/L pyraclostrobin, then is entirely insertable in flower mud and is placed in artificial climate training
Feeding case, and it is 20 DEG C that incubator temperature, which is arranged, air humidity 80%, intensity of illumination 7000lux, root system to be begun with is grown
Afterwards, it moves it into nutrient solution and cultivates wait select, nutrient solution is that Japanese garden formula formula nutritional liquid adds 800 times of earthworm enzyme polypeptide plants
Antibacterial nutrient solution, having chosen 1 plant of root quantity is 11, and longest root length is that the laurustinus Rooted Cuttings of 6.7cm are stand-by；
Step 2: dark processing
The laurustinus Rooted Cuttings of selection are placed in dark culturing case, and it is 14 DEG C that incubator temperature, which is arranged, is carried out at dark
Manage 36h；
Step 3: lighting process
The laurustinus Rooted Cuttings that dark processing terminates are placed in the direct projection 60min under sunlight.Temperature is 25 DEG C when processing；
Step 4: fixed microscopy
The laurustinus Rooted Cuttings that lighting process terminates are taken into the tip of a root, according to cytology flaking method, the tip of a root is sampled
And it is fixed, fixed solution is Kano fixer (acetic acid: alcohol=3:1 (v/v)).And microscopy sight is carried out under phase contrast microscope
It examines, examines the tip of a root to have silk by the method for the cell number with mitosis metaphase phase chromosome in statistics chromosome sectioning
Whether metaphase Phase Proportion is improved.
Embodiment 2
Step 1: raw material is chosen
The top tip of 10 4cm long is taken to be cutting and retain 3 leaves in top from 1 plant of healthy laurustinus maternal plant first, greatly
Blade, which is cut, stays 1/3, is then placed in its whole and is mixed with 60mg/L methyl α-naphthyl acetate, 60mg/L indolebutyric acid, 0.086mg/L biphenyl hydrazine
5min is impregnated in the treatment fluid of ester and 0.13mg/L pyraclostrobin, then is entirely insertable in flower mud and is placed in artificial climate training
Feeding case, and it is 25 DEG C that incubator temperature, which is arranged, air humidity 85%, intensity of illumination 10000lux, root system to be begun with is long
It after out, moves it into nutrient solution and cultivates wait select, nutrient solution is that Japanese garden formula formula nutritional liquid adds 800 times of earthworm enzyme polypeptides plants
The antibacterial nutrient solution of object, having chosen 1 plant of root quantity is 15, and longest root length is that the laurustinus Rooted Cuttings of 5cm are stand-by；
Step 2: dark processing
The laurustinus Rooted Cuttings of selection are placed in dark culturing case, and it is 15 DEG C that incubator temperature, which is arranged, is carried out at dark
Manage 40h；
Step 3: lighting process
The laurustinus Rooted Cuttings that dark processing terminates are placed in lighting process 60min under LED lamplight, LED lamplight is specifically
Light quantum flux is 60 ± 5 μm of ol/m2/s, and white light: green light=3:2 LED lamplight.Temperature is 25 DEG C when processing；
Step 4: fixed microscopy
The laurustinus Rooted Cuttings that lighting process terminates are taken into the tip of a root, according to cytology flaking method, the tip of a root is sampled
And it is fixed, fixed solution is Kano fixer (acetic acid: alcohol=3:1 (v/v)).And microscopy sight is carried out under phase contrast microscope
It examines, examines the tip of a root to have silk by the method for the cell number with mitosis metaphase phase chromosome in statistics chromosome sectioning
Whether metaphase Phase Proportion is improved.
Embodiment 3
Step 1: raw material is chosen
The top tip of 10 5cm long is taken to be cutting and retain 4 leaves in top from one plant of healthy laurustinus maternal plant first, greatly
Blade, which is cut, stays 1/2, is then placed in its whole and is mixed with 60mg/L methyl α-naphthyl acetate, 60mg/L indolebutyric acid, 0.086mg/L biphenyl hydrazine
5min is impregnated in the treatment fluid of ester and 0.13mg/L pyraclostrobin, then is entirely insertable in flower mud and is placed in glasshouse
It is interior, and it is 28 DEG C or so that temperature, which is arranged, air humidity 90%, intensity of illumination 15000lux, after beginning with root system and growing,
It moves it into nutrient solution and cultivates wait select, nutrient solution is that Japanese garden formula formula nutritional liquid adds 800 times of earthworm enzyme polypeptide plant suppressions
Bacterial nutrient solution, having chosen one plant of root quantity is 19, and longest root length is that the laurustinus Rooted Cuttings of 7cm are stand-by；
Step 2: dark processing
The laurustinus Rooted Cuttings of selection are placed in dark culturing case, and it is 16 DEG C that incubator temperature, which is arranged, is carried out at dark
Manage 48h；
Step 3: lighting process
The laurustinus Rooted Cuttings that dark processing terminates are placed in lighting process 60min under LED lamplight, LED lamplight is specifically
Light quantum flux is 60 ± 5 μm of ol/m2/ s, and white light: green light=3:2 LED lamplight.Temperature is 26 DEG C when processing；
Step 4: fixed microscopy
The laurustinus Rooted Cuttings that lighting process terminates are taken into the tip of a root, according to cytology flaking method, the tip of a root is sampled
And it is fixed, fixed solution is Kano fixer (acetic acid: alcohol=3:1 (v/v)).And microscopy sight is carried out under phase contrast microscope
It examines, examines the tip of a root to have silk by the method for the cell number with mitosis metaphase phase chromosome in statistics chromosome sectioning
Whether metaphase Phase Proportion is improved.
Conclusion: the chromosome sectioning made from existing conventional method passes through mirror almost without division phases in every film-making
Inspection finds that the metacinesis mid-term in chromosome sectioning made from embodiment 1-3 mutually reaches 10 or so, and metaphase compares
It is good more visible, as shown in Figure 1, it was confirmed that the method for the present invention can be significantly improved really in the tip of a root mitosis of laurustinus Rooted Cuttings
Phase phase provides the material of high quality for the laurustinus karyotyping in later period and FISH analysis research, and all
In operating process, the harmful chemicals of severe toxicity is not used, so that the risk of experimentation is small, it is environmentally friendly, it is worth pushing away
Extensively.
Claims (10)
1. a kind of method for improving laurustinus tip of a root mitosis metaphase phase, which comprises the following steps:
Step 1: raw material is chosen
Selection root quantity is 10-20 item, and root length is that the laurustinus Rooted Cuttings of 5-7cm are stand-by；
Step 2: dark processing
The laurustinus Rooted Cuttings of selection are placed in progress dark processing 36-48h at 14 DEG C -16 DEG C；
Step 3: lighting process
The laurustinus Rooted Cuttings that dark processing terminates are placed in progress lighting process 60min at 24 DEG C -26 DEG C；
Step 4: fixed microscopy
Take the tip of a root that rear microscopy is fixed the laurustinus Rooted Cuttings that lighting process terminates.
2. the method according to claim 1 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that step 1
In selected laurustinus Rooted Cuttings pre-processed by following steps, the top of 3-5cm long is taken from laurustinus maternal plant first
The tip is cutting and does thin leaf processing, is then inserted into flower mud, and it is 20-28 DEG C that temperature, which is arranged, air humidity 80-
90%, intensity of illumination 7000-15000lux is finally moved it into nutrient solution and is cultivated wait select after having root system to grow.
3. the method according to claim 1 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that step 3
The light source of middle lighting process is direct sunlight.
4. the method according to claim 1 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that step 3
The light source of middle lighting process is LED lamplight.
5. the method according to claim 4 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that LED light
Light is specifically that light quantum flux is 60 ± 5 μm of ol/m2/ s, and white light: green light=3:2 LED lamplight.
6. the method according to claim 1 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that at dark
Managing temperature is 15 DEG C, and lighting process temperature is 25 DEG C.
7. the method according to claim 2 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that described to insert
The concrete operation step that item dredges leaf processing is to retain top 2-4 piece leaf, and big blade, which is cut, stays 1/3-1/2.
8. the method according to claim 2 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that by cutting
Be inserted into flower mud before, place it in be mixed with 60mg/L methyl α-naphthyl acetate, 60mg/L indolebutyric acid, 0.086mg/L Bifenazate and
5min is impregnated in the treatment fluid of 0.13mg/L pyraclostrobin.
9. the method according to claim 2 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that flower mud
Length, width and height are respectively 0.7cm, 0.7cm and 2cm.
10. the method according to claim 2 for improving laurustinus tip of a root mitosis metaphase phase, which is characterized in that described
Nutrient solution is that Japanese garden formula formula nutritional liquid adds 800 times of antibacterial nutrient solutions of earthworm enzyme polypeptide plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910805237.XA CN110447402A (en) | 2019-08-29 | 2019-08-29 | A method of improving laurustinus tip of a root mitosis metaphase phase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910805237.XA CN110447402A (en) | 2019-08-29 | 2019-08-29 | A method of improving laurustinus tip of a root mitosis metaphase phase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110447402A true CN110447402A (en) | 2019-11-15 |
Family
ID=68489714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910805237.XA Pending CN110447402A (en) | 2019-08-29 | 2019-08-29 | A method of improving laurustinus tip of a root mitosis metaphase phase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110447402A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1891033A (en) * | 2005-07-01 | 2007-01-10 | 上海种业（集团）有限公司 | Method for semi-water-culture ceramicite tissue culture of flower |
| CN105284562A (en) * | 2014-07-14 | 2016-02-03 | 上海梵云园艺科技有限责任公司 | Inducing method for water roots of plants |
| CN109197486A (en) * | 2018-07-16 | 2019-01-15 | 云南鑫源花卉种植有限公司 | A kind of laurustinus cuttage and seedling culture method |
| CN109856330A (en) * | 2019-01-29 | 2019-06-07 | 南京农业大学 | A method of chrysanthemum tip of a root Mitotic phase is improved by artificial regulatory |
-
2019
- 2019-08-29 CN CN201910805237.XA patent/CN110447402A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1891033A (en) * | 2005-07-01 | 2007-01-10 | 上海种业（集团）有限公司 | Method for semi-water-culture ceramicite tissue culture of flower |
| CN105284562A (en) * | 2014-07-14 | 2016-02-03 | 上海梵云园艺科技有限责任公司 | Inducing method for water roots of plants |
| CN109197486A (en) * | 2018-07-16 | 2019-01-15 | 云南鑫源花卉种植有限公司 | A kind of laurustinus cuttage and seedling culture method |
| CN109856330A (en) * | 2019-01-29 | 2019-06-07 | 南京农业大学 | A method of chrysanthemum tip of a root Mitotic phase is improved by artificial regulatory |
Non-Patent Citations (1)
| Title |
|---|
| 姜锡瑞等: "《生物发酵产业技术》", 31 May 2016, 中国轻工业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104521539A (en) | Gold leaf acer negundo twig cutting seedling method | |
| CN103782790A (en) | Method for cultivating saffron buds | |
| CN106034936A (en) | Magnolia denudate pot cultivation method | |
| CN101574034B (en) | Spring festival peony culture method | |
| CN100484398C (en) | In vitro conservation method of churysanthemum by reducing macroelements in the medium | |
| CN103478028A (en) | Directional cultivation method of white or purple fresh water pearl | |
| CN100574612C (en) | A kind of method of chrysanthemum germ plasm resource {in vitro} conservation | |
| CN102487698A (en) | Method for promoting chrysanthemum to bloom in advance by using 5-azaC | |
| CN105613060A (en) | Velvet crape myrtle top grafting seedling growing method | |
| CN102657071A (en) | Method for culturing phytoplankton with predominant chlorophyta | |
| CN106069050A (en) | The breeding method that a kind of Flos Gardeniae is potted plant | |
| CN104215485A (en) | Flaking method of petunia chromosome | |
| CN106613994A (en) | Method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources | |
| CN104012416A (en) | Method for efficiently regenerating Sapium sebiferum leaf disc | |
| CN110447402A (en) | A method of improving laurustinus tip of a root mitosis metaphase phase | |
| CN105027873A (en) | Plumbago auriculata shoot cutting propagation method | |
| CN102726302B (en) | Dark culture method for banana tissue culture | |
| CN107311745A (en) | A kind of white orchid planting soil | |
| CN108323425A (en) | A kind of cuttage breeding method of four seasons asoka | |
| CN115039655A (en) | Standardized potted flower cultivation method for foliage begonia | |
| CN205511119U (en) | Roof flower planting culture apparatus | |
| CN102584441A (en) | Cultivation matrix and cultivation method for zantedeschia hybrida cut flower | |
| CN104956867B (en) | A kind of Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait leaf is spent with the method for potted put | |
| CN214126194U (en) | Flowers artificial containers is used in gardens | |
| CN101112171B (en) | Method applied in the north region for cultivating anthurium andraeanum seedling through water cuvette and substrate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191115 |