CN110393164B - Three-stage cultivation method for large-size clam seedlings suitable for cold sea areas in northern China - Google Patents
Three-stage cultivation method for large-size clam seedlings suitable for cold sea areas in northern China Download PDFInfo
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Abstract
The invention discloses a three-stage cultivation method of large-size clam seedlings, which is suitable for cold sea areas in northern China, and comprises the following steps: the method comprises the following steps of clam parent shell screening, spawning, fertilized egg hatching, planktonic larva indoor artificial breeding, metamorphosis and attachment of larval on the top of a shell and the like, wherein young clams with the shell length of about 0.9mm are bred from the end of 6 months to the middle of 10 months; and a second stage: carrying out overwintering cultivation on young clams of the clams in an outdoor pond until the shell length is about 4.5mm, wherein the time is from 10 in the middle of ten days to 4 months in the next year; and a third stage: the process of culturing the overwintering young clams in the outdoor pond until the shell length is more than 1.0cm, wherein the time course is about from 4 months to 7 months. The method can provide technical support for large-scale production of large-size clam seedlings and provide sufficient seedling supply for development of clam breeding industry.
Description
Technical Field
The invention relates to a shellfish fry breeding technology, in particular to a three-stage breeding method for large-size clam fries in cold sea areas in northern China; is especially suitable for cultivation in the area of Dalian province.
Background
The hard clam (Meretrix Meretrix) belongs to the phylum mollusca, the class of lamellibranchia, the order of veneridae, the family of veneridae and the genus of hard clam, and is one of the important marine economic shellfish in China. The clams are mainly distributed in coastal areas such as Liaoning, Shandong, Jiangsu, Guangxi and Taiwan in China, and in gulf and near estuary in coastal areas of Korea and Japan. Some japanese scholars have developed some progress in indoor artificial breeding and proliferation tests of clams as early as the 20 th century from the end of the 70 s to the beginning of the 80 s, but the indoor artificial breeding and proliferation tests are still on a test scale up to now. At the end of the 20 th century 70 s, the Xiamen aquatic academy and the Jiangsu province marine aquatic research institute led domestic clam indoor artificial breeding research, mainly focused on artificial induced spawning, larva breeding ecological conditions and palatable bait screening and other research works, and made great progress, but failed to solve the problem of intermediate breeding of young clam. In the 90 s of the 20 th century, researches on industrial clam breeding technologies were successively carried out by a marine aquatic product research institute of Shandong province, a marine aquatic product research institute of Liaoning province, a marine aquaculture research institute of Zhejiang province, a marine research institute of Chinese academy of sciences and a stock ground of Panshan clams, and indoor artificial clam breeding technologies were further perfected. Meanwhile, the Liaoning province marine aquatic product research institute carries out the productive indoor or outdoor overwintering temporary culture test of the young clams, and has great progress in the overwintering technology of the young clams, but the large-scale production is not completely realized. In the early stage cultivation process of the clam fries in the south-to-south sea area of Shandong, China, a two-stage cultivation technical system is generally adopted, namely an indoor artificial seedling cultivation-outdoor pond intermediate cultivation way. However, the production practice proves that the two-section clam seedling cultivation method is not suitable for the large-scale production of large-size clam seedlings in cold sea areas in northern China.
Disclosure of Invention
The invention aims to provide a large-specification clam seedling cultivation method suitable for cold sea areas in northern China, namely a three-stage large-specification clam seedling cultivation method for artificial cultivation of indoor seedlings, overwintering cultivation in outdoor ponds and intermediate cultivation in outdoor ponds, so that large-specification clam seedlings can be produced on a large scale.
In order to achieve the purpose, the technical scheme of the invention is as follows: a three-stage cultivation method for large-size clam seedlings in cold sea areas in northern China comprises the following steps:
s1, indoor culture period: from the end of 6 months to 10 middle months;
selecting individuals with gonad development to IV stage, 3-5 age and 6-8 cm shell length as parent shells; putting the fertilized eggs into a spawning pond for spawning and sperm discharging, controlling the hatching density of the fertilized eggs to be 30-50/mL, stirring pond water for 1 time every 1 hour during hatching, and keeping the hatching water temperature to be 25-27 ℃;
then, the D-shaped larvae are cultivated in a still water or micro-aeration mode, the cultivation density is 5-8/mL, the larvae are kept in a 500lux low light state during cultivation, water is changed for 1-2 times every day, the daily water amount is 2/3-3/4 of the water body, 4-6 times of bait feeding are carried out every day, and the bait is 1 x 10 chrysophyceae4～2×104cell/ml, Chlorella 3X 105～5×105cell/ml and 9X 10 Nitzschia closterium4～18×104Mainly taking cell/ml; until the larval stage of the shell top, the breeding density is 8.0 multiplied by 105Per m2The water depth is 1.2 meters, 1.0-1.5 cm of fine sand is put in, a water gun is used for washing the sand layer at the bottom of the pool every day, 4-6 times of feeding are carried out every day, and the feeding mainly comprises golden algae and flat algae; culturing until the average shell length is 0.9 mm;
in the young clam period, the young clams are moved to an outdoor pond, and the putting density is about 6.0 multiplied by 105Piece/m2The water depth is 1.2 m, the bait is flat algae 0.1X 104～0.2×104cell/ml and Chlorella 1X 105～2×105Mainly cell/ml, and simultaneously assisted by 1.5 × 10 of chrysophyceae4～2.0×104cell/ml and Chaetoceros muelleri 1X 104～2×104controlling the temperature of the culture water to be 23-26 ℃, changing water once every day, and flushing a sand layer by using a water gun;
s2, outdoor culture period: from 10 middle of the month to 4 months of the second year;
at this stage, the young clam is cultivated through the winter in the outdoor pond until the shell length is about 4.5 mm;
the outdoor pond is arranged in a net cage mode, and each net cage comprises a cement strip block paved at the bottom, a bamboo curtain paved on the cement strip block, a first nylon bolting silk net layer with 4 meshes paved on the bamboo curtain, a second nylon bolting silk net layer with 8 meshes paved on the first nylon bolting silk net layer, and fine sand paved at the uppermost layer;
vertical support rods are arranged at intervals around the net cage, and a third nylon bolting silk net of 120 meshes and a fourth nylon bolting silk net of 20 meshes which surround the pond for a circle are sequentially fixed through the support rods from inside to outside by using cables;
s3, culture period after overwintering: from month 4 to month 7;
and continuously culturing the seeds in the outdoor pond until the length of the shell is more than 1.0 cm.
In a preferred mode, the specification of the cement bar block is 6.0 multiplied by 4.0 m; the third nylon bolting silk net and the fourth nylon bolting silk net are 20c and 30cm higher than the surface of the fine sand respectively; the distance between the adjacent support rods is 1 m; the fine sand thickness is 2 cm.
Step S2 in the optimal mode, the water level of the outdoor pond is raised before the outdoor pond is cultured in winter and is sealed with ice, and the water depth is more than 2.0 m-2.5 m; 1 time of ice hole drilling every half month after ice sealing; removing snow on ice after snowing.
In addition, in a preferable mode, in step S2, water is changed for 1 time every two days, the salinity of the seawater should be maintained at 25-28 per mill, young shellfish individuals are sorted in time, and clam fries of different specifications are bred respectively.
The invention has the following advantages:
the three-stage cultivation method of the large-specification clam seedlings can enable the overwintering survival rate of the large-specification clam seedlings to reach 85%, and breaks through the technical bottleneck that the clam seedlings in cold sea areas in China are difficult to cultivate, so that the cultivation technical system of the large-specification clam seedlings in northern sea areas in China is further improved, technical support is provided for large-scale production of the large-specification clam seedlings, and sufficient seed supply is provided for the development of the clam breeding industry.
Drawings
FIG. 1 is a schematic view of the overwintering cultivation facility according to the present invention;
FIG. 2 is an enlarged detail view taken at circle in FIG. 1;
in the figure: 1. cement bar block, 2, bamboo curtain, 3, 4 mesh nylon screen silk net, 4, 8 mesh nylon screen silk net, 5, fine sand, 6, 120 mesh nylon screen silk net, 7, 20 mesh nylon screen silk net, 8, mooring rope, 9, knot, 10, support rod
Detailed Description
The invention firstly provides a pond suitable for northern outdoor culture of clams, as shown in figures 1 and 2, the overwintering culture facility is a land-based enclosure constructed by upper and lower enclosure nets, and the main body part of the overwintering culture facility mainly comprises a cement bar block 1, a bamboo curtain 2, a 4-mesh nylon bolting silk net 3, an 8-mesh nylon bolting silk net 4, a 120-mesh nylon bolting silk net 6, a 20-mesh nylon bolting silk net 7, fine sand 5, a mooring rope 8, a knot 9 and a support rod 10.
The bottom of the pond is formed by combining the cement strips 1, the specification is 6.0 multiplied by 4.0m, a bamboo curtain 2 woven by bamboo is laid on the bottom of the pond, and two layers of 4-mesh nylon screen silk net 3 and 8-mesh nylon screen silk net 4 are respectively placed above the bamboo curtain 2; the upper layer netting and the lower layer netting are rectangular, the 20-mesh nylon screen silk net and the 120-mesh nylon screen silk net are adopted respectively, the heights are respectively 30cm and 20cm, and the upper layer netting and the lower layer netting are sewn in advance by a sewing machine; the netting is fixed on the support rod at intervals of 1m around the rectangular direction by tying bands, and the upper edge and the lower edge of the netting are sewn in the netting by cables to reinforce the netting. After the netting is fixed, fine sand with the thickness of 2cm (filtered by a 80-mesh silk net) is put on the bottom layer netting.
The invention provides a three-stage cultivation method for large-size clam seedlings by utilizing the pond, which comprises the following steps: the method comprises the following steps of clam parent shell screening, spawning, fertilized egg hatching, planktonic larva indoor artificial breeding, metamorphosis and attachment of larval on the top of a shell and the like, wherein young clams with the shell length of about 0.9mm are bred from the end of 6 months to the middle of 10 months; and a second stage: carrying out overwintering cultivation on young clams of the clams in an outdoor pond until the shell length is about 4.5mm, wherein the time is from 10 in the middle of ten days to 4 months in the next year; and a third stage: the process of culturing the overwintering young clams in the outdoor pond until the shell length is more than 1.0cm, wherein the time course is about from 4 months to 7 months. The method can provide technical support for large-scale production of large-size clam seedlings and provide sufficient seedling supply for development of clam breeding industry.
The invention particularly relates to a three-stage cultivation method of large-size clam seedlings in cold sea areas in northern China, which is characterized by comprising the following steps:
1. selecting individuals with gonad development to IV stage, 3-5 age and 6-8 cm shell length as parent shells;
2. placing parent clams with mature neutral glands of the S1 clams into a spawning pond, spawning and discharging sperms, controlling the hatching density of fertilized eggs within the range of 30-50/mL, stirring pond water for 1 time every 1h during hatching, and keeping the hatching water temperature at 25-27 ℃;
3. d-shaped larvae are cultivated in a still water or micro-aeration mode, the cultivation density is 5-8/mL, the larvae are kept in a low light state (500lux) during cultivation, water is changed for 1-2 times every day, the daily water amount is 2/3-3/4 of a water body, bait is thrown for 4-6 times every day, and the bait is 1 x 10 of chrysophyceae4～2×104cell/ml, Chlorella 3X 105～5×105cell/ml and 9X 10 Nitzschia closterium4～18×104Mainly taking cell/ml;
4. the breeding density of the shell top larva is 8.0 multiplied by 105Per m2The water depth is 1.2 meters, 1.0-1.5 cm of fine sand is put in, a water gun is used for washing a sand layer at the bottom of the pool every day, 4-6 times of feeding are carried out, the feeding is mainly made of golden algae and flat algae, and the amount of the feeding is adjusted according to the color, the fullness degree and the residual feeding amount of the larvae in the caecum;
5. during the period of cultivating young clam, the throwing density is about 6.0 x 105Piece/m2The bait is made of flat algae 0.1X 104～0.2×104cell/ml and Chlorella as main materials, and Chlorella 1.5 × 10 as adjuvant materials4～2.0×104cell/ml and Chaetoceros muelleri 1X 104～2×104The cell/ml is cultured at the temperature of 23-26 ℃, water is changed once every day by 100%, and a sand layer is washed by a high-pressure gun, so that the aims of discharging residual bait, excrement and cleaning mucus are fulfilled;
as shown in fig. 1 and 2, the outdoor pond overwintering cultivation facility comprises a cement bar block 1, a bamboo curtain 2, a 4-mesh nylon screen silk net 3, an 8-mesh nylon screen silk net 4, fine sand 5, a 120-mesh nylon screen silk net 6, a 20-mesh nylon screen silk net 7, a cable 8, a knot 9 and a support rod 10. The bottommost part of the device consists of the cement bar blocks, the bamboo curtain, the 4-mesh nylon bolting silk net and the 8-mesh nylon bolting silk net, a first layer of nylon bolting silk net 3 is laid on the top of the device, a second layer of nylon bolting silk net 4 is laid above the first layer of nylon bolting silk net 3, fine sand is laid on the uppermost layer, the thickness of the fine sand is 2cm, the periphery of the device is formed by surrounding the 120-mesh nylon bolting silk net and the 20-mesh nylon bolting silk, and the bolting silk nets on the periphery are fixed on the support rod through the mooring rope and the rope knot; the outdoor pond intermediate cultivation facility is completely consistent with the outdoor pond overwintering cultivation facility.
Wherein, the water level is increased as much as possible before the outdoor pond overwintering cultivation needs to enter the winter and be sealed with ice, the water depth is more than 2.0m, four water quality physicochemical indexes of water temperature, salinity, dissolved oxygen and ammonia nitrogen are measured every week, the substrate condition (whether the substrate is smelly and the color of the substrate mud) of the overwintering pond and the survival condition of seedlings (whether dead individuals exist) are checked at intervals of half a month, 1 time of ice eyes are drilled every half a month after ice sealing, and accumulated snow on the ice is removed in time after snow falls; the intermediate cultivation of the outdoor pond needs to increase the water change amount during the cultivation period of the young clams, water is changed for 1 time every two days, the growth of the young clams is promoted by a proper water fertilizing or flowing mode, the salinity of seawater is kept between 25 per thousand and 28 per thousand, young clam individuals are sorted in time, and the young clams with different specifications are cultivated respectively.
Example 1
250kg of parent scallops with mature gonads are transported to a breeding field in 2016, 7 and 5 days, and then enter a pool for spawning and spermiation, and when the spawning of parent scallops reaches the breeding density, the parent scallops are transferred to other spawning pools for continuous spawning. Uniformly stirring fertilized eggs in the pond, sampling, then filling the pond with water for incubation, wherein the incubation density of the fertilized eggs is about 45/mL, so that 20 hundred million fertilized eggs are obtained, stirring the pond water for 1 time every 1 hour during incubation, and the incubation water temperature is about 25 ℃; the cultivation density of the D-shaped larvae is about 5.5/mL, the larvae are cultivated by micro-aeration, 50% of water is changed twice every day, and the concentration of the larvae is 2-3 g/m after the water is changed every day3The penicillin antibacterial drug is fed for 6 times every day, golden algae, chlorella and nitzschia closterium are taken as main materials, and when the average shell length of planktonic larvae reaches 150-160 mu m, the penicillin antibacterial drug is poured into a pool; the larva at the top of the shell is divided into 80 multiplied by 104Per m2Transferring the density into a culture pond, simultaneously putting 1.5cm of the treated fine sand, controlling the water level of the culture pond at 50cm, aerating, changing water for 1 time every day at 100%, flushing the fine sand by using a water gun when changing water, feeding the golden algae and the Platymonas subcordata for 5 times every day, and properly adjusting the bait amount according to the color of the water body; the young clam breeding density is about 60 multiplied by 104Piece/m2The bait mainly comprises Platymonas and Chlorella,replacing water with Jincai and Chaetoceros muelleri as auxiliary materials at 100% per day for 1 time, and washing sand layer with water gun; 8600 ten thousand grains of Meretrix meretrix Linnaeus with shell length of 700-1500 mu m are obtained in 10 months and 15 days.
Carrying out overwintering cultivation on clam seedlings in an outdoor pond from 10 months to 2017 months in 2016, wherein the area of the pond is about 0.27hm 210 net cages with the specification of 6.0 multiplied by 4.0 multiplied by 0.5m (length multiplied by width multiplied by height) are placed in total, the specification of the netting used at the bottom of the net cage is 120 meshes, the meshes used at the periphery are 120 meshes and 20 meshes in sequence, and the height is 20cm and 30cm respectively. Meanwhile, a net cage placing platform is made of cement strips at the bottom of the pond, a bamboo curtain woven by bamboo is laid on the net cage placing platform, two layers of net clothes with meshes of 4 meshes and 8 meshes are laid above the bamboo curtain, and the indoor cultured clam seedlings are uniformly sowed into the net cage according to the density of about 1000 ten thousand grains per cage; before the ice is sealed in winter, the water level is increased to 2.5m, four water quality indexes of water temperature, salinity, dissolved oxygen and ammonia nitrogen are measured every week, the substrate condition and the seedling survival condition are checked, ice holes are opened for 1 time every half month after the ice is sealed, and accumulated snow on the ice is removed in time; in 12 days 4 months in 2011, the juvenile clam after overwintering is measured, the average shell length of the young clam is about 4.5mm, the total number of the young clam is about 7100 ten thousand grains, and the overwintering survival rate is about 83 percent.
During the period of 4 months to 8 months in 2017, the clams are put into an outdoor shrimp pond for intermediate cultivation, 2 ponds are shared, and the area is 0.07hm2Left and right. 10 net cages with the specification of 6.0 multiplied by 4.0 multiplied by 0.5m are arranged in each pond, the net cages are placed on a built platform for fixing, fine sand which is 2cm thick and is filtered by a 80-mesh silk net is placed in each net cage, and a water pump with the diameter of 5cm is used for cleaning; disinfecting the breeding pond by using bleaching powder before throwing seedlings, manually removing enemies of snails and crabs, then feeding water to 1m, fertilizing water, controlling the transparency to be 30-50 cm, and adding the water level to 1.5 m; uniformly sowing the seedlings into a net cage according to the density of about 100 ten thousand seeds per cage when putting the seedlings; the management is mainly divided into three periods, the fry is washed by a water pump with 5cm for 1 time every 7 days in the early period of breeding (4-5 months), and the water is changed for 1 time every two days, wherein the water change amount is 1/2; in the middle breeding period (5-6 months), washing the seedlings for 1 time every 5 days, and changing water for 1 time every two days, wherein the water changing amount is 1/2; the management at the late breeding stage (7-8 months) is basically consistent with the middle breeding stageFlushing a sand layer in the net cage by using a submersible pump with the diameter of 5.1cm, periodically measuring water temperature and salinity water quality physicochemical factors in an intermediate cultivation stage, and checking the change condition of a substrate and the survival condition of seedlings; average shell length obtained in 8 months in 2017>About 1150 ten thousand fries of 1.3cm, about 550 ten thousand fries of less than 1.3cm and about 85 percent of survival rate of the intermediate breeding of the young clam.
The three-stage cultivation method of the large-size clam fingerlings suitable for the cold sea areas in northern China provided by the invention can greatly improve the survival rate of the young clams. Due to limited space, the whole test data cannot be enumerated, and the invention only provides partial test data to support the beneficial effect of the invention.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (3)
1. A three-stage cultivation method for large-size clam seedlings in cold sea areas in northern China is characterized by comprising the following steps:
s1, indoor culture period: from the end of 6 months to 10 middle months;
selecting individuals with gonad development to IV stage, age of 3-5 and shell length of 6-8 cm as parent scallops; putting the fertilized eggs into a spawning pond for spawning and sperm discharging, controlling the hatching density of the fertilized eggs to be 30-50/mL, stirring pond water for 1 time every 1 hour during hatching, and keeping the hatching water temperature to be 25-27 ℃;
then, the D-shaped larvae are cultivated in a still water or micro-aeration mode, the cultivation density is 5-8/mL, the larvae are kept in a 500lux low light state during cultivation, water is changed for 1-2 times every day, the daily water change amount is 2/3-3/4 of the water body, bait is thrown for 4-6 times every day, and the bait is 1 x 10 chrysophyceae4~2×104cell/ml, Chlorella 3X 105~5×105cell/ml and 9X 10 Nitzschia closterium4~18×104Mainly taking cell/ml; until the larval stage of the shell top, the breeding density is 8.0 multiplied by 105Per m2The water depth is 1.2 meters, 1.0-1.5 cm of fine sand is put in, a water gun is used for washing the sand layer at the bottom of the pool every day, 4-6 times of feeding are carried out every day, and the feeding mainly comprises golden algae and flat algae; culturing until the average shell length is 0.9 mm;
in the juvenile period of the clams, the juvenile clams are moved to an outdoor pond, and the putting density is 6.0 multiplied by 105Piece/m2The water depth is 1.2 m, the bait is flat algae 0.1X 104~0.2×104cell/ml and Chlorella 1X 105~2×105Mainly cell/ml, and simultaneously assisted by 1.5 × 10 of chrysophyceae4~2.0×104cell/ml and Chaetoceros muelleri 1X 104~2×104controlling the temperature of the culture water to be 23-26 ℃, changing water once every day, and flushing a sand layer by using a water gun;
s2, outdoor culture period: from 10 middle of the month to 4 months of the second year;
at this stage, juvenile clams of the clams are cultivated through overwintering in an outdoor pond until the shell length is 4.5 mm;
the outdoor ponds are arranged in a net cage mode, each net cage comprises a cement strip block (1) paved at the bottom, a bamboo curtain (2) paved on the cement strip block (1), a first nylon bolting silk net (3) of 4 meshes paved on the bamboo curtain (2), a second nylon bolting silk net (4) of 8 meshes paved on the first nylon bolting silk net (3), and fine sand (5) paved on the uppermost layer;
vertical support rods (10) are arranged at intervals around the net cage, and a third nylon bolting silk net (6) of 120 meshes and a fourth nylon bolting silk net (7) of 20 meshes which surround the pond for a circle are sequentially fixed from inside to outside through the support rods (10) by using a mooring rope (8);
s3, culture period after overwintering: from month 4 to month 7;
continuing the outdoor pond culture until the shell length is more than 1.0 cm;
the specification of the cement strip block (1) is 6.0 multiplied by 4.0 m; the third nylon bolting silk net (6) and the fourth nylon bolting silk net (7) are respectively 20cm and 30cm higher than the surface of the fine sand (5); the distance between the adjacent support rods (10) is 1 m; the thickness of the fine sand (5) is 2 cm.
2. The three-stage cultivation method of large-size clam seedlings suitable for cold sea areas in northern China according to claim 1, wherein in step S2, the water level of outdoor pond overwintering cultivation is raised before entering winter and ice sealing, and the water depth is more than 2.0 m-2.5 m; 1 time of ice hole drilling every half month after ice sealing; removing snow on ice after snowing.
3. The three-stage cultivation method of large-size clam fry suitable for cold sea areas in northern China as claimed in claim 1, wherein in step S2, water is changed 1 time every two days, the salinity of seawater is maintained at 25-28 per mill, young clam individuals are sorted in time, and clam fries of different specifications are cultivated respectively.
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| CN116250498A (en) * | 2022-12-14 | 2023-06-13 | 大连海洋大学 | Philippine clams young offspring seed intermediate cultivation method suitable for northern sea area |
| CN117882663B (en) * | 2024-03-14 | 2024-05-28 | 山东得和明兴生物科技有限公司 | Automatic high-density clam offspring breeding system and method |
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| CN101637140A (en) * | 2009-07-31 | 2010-02-03 | 山东省海洋水产研究所 | Indoor wintering method of octopus parents in north |
| CN106719184A (en) * | 2016-12-29 | 2017-05-31 | 丹东市水产技术推广总站 | A kind of artificial breeding method of Mactra chinensis |
| CN107969362A (en) * | 2017-12-14 | 2018-05-01 | 日照市海洋与渔业研究所(日照市海域使用动态监视监测中心、日照市水生动物救护站) | Purple coloured silk blood clam seed method for scaled breeding |
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2019
- 2019-06-28 CN CN201910583533.XA patent/CN110393164B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1401225A (en) * | 2002-09-23 | 2003-03-12 | 盘山县水产技术推广站 | Method for overwintering and intermidiate breeding clam fry |
| CN101637140A (en) * | 2009-07-31 | 2010-02-03 | 山东省海洋水产研究所 | Indoor wintering method of octopus parents in north |
| CN106719184A (en) * | 2016-12-29 | 2017-05-31 | 丹东市水产技术推广总站 | A kind of artificial breeding method of Mactra chinensis |
| CN107969362A (en) * | 2017-12-14 | 2018-05-01 | 日照市海洋与渔业研究所(日照市海域使用动态监视监测中心、日照市水生动物救护站) | Purple coloured silk blood clam seed method for scaled breeding |
Non-Patent Citations (2)
| Title |
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| 《文蛤稚贝室外越冬技术的研究》;陈远等;《水产科学》;20021130;第21卷(第6期);第16-17页 * |
| 《文蛤苗种繁育、越冬及中间育成关键技术的突破与展望》;陈远等;《河北渔业》;20121031(第10期);第47-66页 * |
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