CN110357345A - One breeding class feces of farm recycling bioremediation - Google Patents
One breeding class feces of farm recycling bioremediation Download PDFInfo
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- CN110357345A CN110357345A CN201910442478.2A CN201910442478A CN110357345A CN 110357345 A CN110357345 A CN 110357345A CN 201910442478 A CN201910442478 A CN 201910442478A CN 110357345 A CN110357345 A CN 110357345A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/12—Treatment of sludge; Devices therefor by de-watering, drying or thickening
- C02F11/121—Treatment of sludge; Devices therefor by de-watering, drying or thickening by mechanical de-watering
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention provides a breeding class feces of farm recycling bioremediation, this method realizes the Bi-objective of pollutant process and resource utilization by the way that waste is resolved into the method that excrement slag and waste water carry out classification processing Concentrated fermentation.Nutrient in liquid dung is remained in excrement slag by microbial flocculation effect, treated, and liquid dung has reached country to the discharge standard of aquaculture waste water, excrement slag is high by the fertilizer nutrient content of high temperature, superhigh temperature fermenting and producing, and weed seed is few, and suit large area to popularize application.This method operating cost is low, and added value is high, does not generate secondary pollution, can be an advanced waste recycling processing method with effectively save water resource.
Description
Technical field
The present invention relates to microorganisms technical fields, specially a breeding class feces of farm recycling bioremediation.
Background technique
Scale domestic animals aquaculture development meets the ever-increasing domestic animals product demand of the whole society, while also producing big
The breeding waste of amount, domestic animals waste problem become increasingly conspicuous.Currently, the colony house of large-scale domestic animals farm mostly uses the clear of water flushing
Clean mode, water backwashing manner can clean cultivation colony house, reduce colony house pollution and poultry colony house infectious disease incidence, operation side
Just, labour cost is saved.But this cleaning mode will cause a large amount of water and rush dunghill product and a large amount of discharge of wastewater, to big
Gas and water body, soil cause greatly to pollute, and feces of farm becomes the main source of agricultural non-point source pollution.Feces of farm
In excrement slag, for fermenting and producing fertilizer, water content is higher (65% or more), is unfavorable for fermenting, total nutrient is very low
(2.6% or so), CN ratio is very high, is unfavorable for fertilizer quality.And the waste water in waste, P content are high, the concentration more than 40mg/L,
Belong to high phosphorus waste water, COD can achieve 8000-20000mg/L, and BOD can achieve 2000-20000mg/L, and it is useless to belong to highly difficult processing
Water, and Normal wastewater processing method will cause a large amount of N, P, K element is lost, and result in waste of resources.
Prior art CN105271610 A discloses a kind of comprehensive processing technique of farm's cow manure wastewater, which exists
In wastewater treatment process plus add flocculant PAM (polyacrylamide) and coagulant PAC(aluminium polychloride), belong to chemical place
Reason method.PAM, PAC's is a large amount of using the increase that will cause processing cost, and these chemical reagent will cause the secondary of environment
Pollution.CN104108840 A discloses a kind of water and rushes excrement formula large-scale milch cow farms waste integrated conduct method, and this method uses heap
Fertilizer processing carries out changing rejected material to useful resource, and this method is more demanding to temperature, and the processing time is long, inefficiency, institute in composting process
Attainable maximum temperature, can be lower in winter at 60 DEG C or so, cannot be thoroughly by pathogen, grass seeds and the soil-borne disease in waste
Insect pest is killed, and disease can be caused to pollute soil, crop and underground water indirectly.And the above-mentioned prior art is micro- using evaporation separation
Biological sludge causes a large amount of P elements to be trapped in waste water, and waste water does not obtain effective purified treatment, while the dirt recycled
Lack P elements in mud, the fertilizer total nutrient content for causing to ferment out is insufficient.
Summary of the invention
The present invention be directed to above-mentioned shortcomings and deficiencies of the prior art, propose at a kind of feces of farm recycling biology
Reason method.
It is provided by the invention by feces of farm recycling bioremediation, specifically comprise the following steps:
1. excrement and liquid dung mixed liquor, which are drained into sedimentation basin, carries out natural sedimentation separation, collection is flowed out after percolation
Percolate, solid portion natural sedimentation is layered to form 2 layers, is at the middle and upper levels solid cow dung layer, lower layer is sandy soils；
2. the percolate that step 1 obtains, which is separated into relatively dry water by solid-liquid separating machine, rushes excrement and extrusion liquid；
3. the resulting extrusion liquid of step 2 is discharged into anaerobic fermentation in the anaerobic pond of the active sludge in bottom, fermentation temperature 36
DEG C, residence time 12h, muddy water ratio 1:3, dissolved oxygen (DO) is horizontal to be not more than 0.2mg/L；
4. the supernatant liquor for completing anaerobic fermentation is carried out aerobic fermentation, microbial bacterial agent is added in 1:1000 by volume, this is micro-
Bacteria agent is the mix bacterium agent of Paenibacillus polymyxa and streptococcus equi, 28 DEG C of fermentation temperature, residence time 12h, is kept molten
It is horizontal higher than 2mg/L to solve oxygen (DO)；
5. mixture is imported sedimentation basin after fermentation, after natural sedimentation 12h, supernatant can direct emission, be precipitated as rich support
Sludge rushes excrement with the water of the solid cow dung layer of step 1 and step 2 and mixes；
6. the moisture content of said mixture is adjusted to 60%, bacillus subtilis and mostly viscous class bud is added according to volume ratio 1:1000
Spore bacillus 1:1 mixed bacteria liquid stirs evenly post-fermentation, and the cool fermentation 10d of natural heap, gained tunning are after 70 DEG C of fermentation 8d
Fertilizer.
Preferably, solid-liquid separating machine described in step 2 is screw extruding type solid-liquid separator.
Preferably, the water content that water described in step 2 rushes excrement is 65-75%.
Preferably, Paenibacillus polymyxa and streptococcus equi are cultivated respectively to logarithmic growth in microbial inoculum described in step 4
Phase, 3:1 was mixed by volume by bacterium solution to obtain the final product.
Preferably, fermentation condition described in step 6 can be the cool fermentation 10d of natural heap after 80 DEG C of fermentation 6d.
Preferably, the Paenibacillus polymyxa is JP-15, the bacterial strain is in submitted country's common micro-organisms preservation
The heart carries out Patent Deposit, culture presevation number: CGMCC No.17230.
Preferably, the streptococcus equi is JP-9, the submitted national common micro-organisms collection of the bacterial strain is carried out
Patent Deposit, culture presevation number: CGMCC No.17231.
The present invention realizes pollution by the way that waste is resolved into the method that excrement slag and waste water carry out classification processing Concentrated fermentation
The Bi-objective of object processing and resource utilization.In the microbial inoculum that aerobic fermentation uses, streptococcus equi is the phosphorus microorganism that birdss of the same feather flock together, can be with
The phosphorus content in feces of farm extrusion liquid is effectively reduced；And more Acarasiales bacillus in microbial inoculum, it is wide due to itself having
The pathogenic microorganism in waste can be killed during the fermentation or be inhibited to the effect for composing sterilization, and the bacterial strain mycelia is flourishing, energy
A large amount of stickums are secreted, the effect of biological flocculant can be played, by the nutrient flocculation in extrusion liquid in the feeding sludge of richness, from
And effectively substitute chemical floc.Waste water in waste can achieve country to aquaculture by the purified treatment of above procedure
The discharge standard of waste water.The feeding sludge of richness and solid cow dung and water rush excrement and are mixed into Preliminary fermentation material, and CN ratio reaches 35, into reason
Think that CN than range, can be improved 5 DEG C of fermentation temperature or so, compost fermentation is made to enter the temperature rise period in advance, it is ensured that high temperature and super
Condition needed for hot fermentation has killed the pathogen in waste and weed seed, while improving the P elements in fertilizer and containing
Amount promotes cellulose degradation to promote decomposed effect, ensure that the fertility of fertilizer.
A kind of feces of farm recycling bioremediation provided by the invention is substituted traditional using microbial bacterial agent
Flocculation medicament is learned, the cost of wastewater treatment is reduced, avoids secondary pollution.Sandy soils after precipitating, it is dry by rinsing sunning
Colony house can be recycled into as animal litter after net.Treated, and waste water has reached country to the discharge mark of aquaculture waste water
Standard can be recycled, save water resource, reduce the cleaning cost of colony house.The poly- phosphorus effect of microbial bacterial agent improves
The utilization rate of nutrient in liquid dung, ensure that the fertility of subsequent fermentation compost fertilizer.Microbial bacterial agent and high temperature, superhigh temperature hair
The collective effect of ferment realizes the harmless treatment of waste, provides guarantee to widely popularize the application of organic fertilizer.
Preservation explanation:
Strain name: Paenibacillus polymyxa
Latin name:Paenibacillus polymyxa
Strain number: JP-15
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on January 24th, 2019
Collection is registered on the books number: CGMCC No. 17230
Strain name: streptococcus equi
Latin name:Streptococcus equines
Strain number: JP-9
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on January 24th, 2019
Collection is registered on the books number: CGMCC No. 17231.
Specific embodiment
Below with reference to specific implementation example, the present invention is described in detail.Following embodiment facilitates the skill in this field
Art personnel further understand the present invention, but the invention is not limited in any way.
Waste experiment sample used in following embodiments, both from the milk of auspicious agriculture limited liability company, China, Gansu Province
Ox plant.
Paenibacillus polymyxa JP-15 culture medium prescription: peptone 5.0g, NH4Cl0.5g, beef extract 10.0g, yeast powder
5.0g, NaCl 5.0g, distilled water 1000ml, pH 7.0.
Streptococcus equi JP-9 culture medium prescription: tryptone 10.0g, yeast extract 5.0g, NaCl10.0g, distillation
Water 1000ml, pH 7.4.
Material as used in the following examples, reagent etc., are commercially available unless otherwise specified.
Embodiment 1
Excrement and liquid dung mixed liquor are drained into sedimentation basin and carry out natural sedimentation separation, liquid is flowed out by percolation.Solid
Part natural sedimentation is layered to form 2 layers, is at the middle and upper levels solid cow dung layer, lower layer is sandy soils；By percolate by solid-liquid point
The water for being separated into and relatively drying of disembarking rushes excrement (solid) and extrusion liquid (fluid)；Measurement solid cow dung layer and water rush containing for excrement respectively
Water measures the total nutrient of mixture, the results are shown in Table 1 after mixing:
1 solid waste basic physical and chemical of table
The experimental results showed that water rushes excrement and the moisture content of solid cow dung layer is high, total nutrient is low, and fermentation quality is low, cannot individually reach
To the standard of fermented organic fertilizer, be not suitable for direct compost fermentation.
Embodiment 2
The resulting extrusion liquid of embodiment 1 is subjected to anaerobic fermentation, i.e., extrusion liquid is discharged into the anaerobic pond of the active sludge in bottom
Middle fermentation, 36 DEG C of fermentation temperature, residence time 12h, muddy water ratio 1:3, dissolved oxygen (DO) is horizontal to be not more than 0.2mg/L；Completion is detested
The supernatant liquor of aerobe fermentation carries out aerobic fermentation, and microbial bacterial agent is added in 1:1000 by volume, which is mostly viscous
The mix bacterium agent of series bacillus and streptococcus equi, keeps DO > 2mg/L by 28 DEG C of fermentation temperature, residence time 12h；It has fermented
Mixture is imported into sedimentation basin after, after natural sedimentation 12h, supernatant direct emission, lower sediment is rich feeding sludge.
A large amount of nutrient element indexs in extrusion liquid, supernatant and rich feeding sludge are detected, experimental result is shown in Table 2.Detection
Every discharge index, experimental result are shown in Table 3 in supernatant.
Each ingredient nutrient content before and after 2 biological treatment of table
3 supernatant discharge index of table
Experimental result is shown in table 2, and the nutrient content in extrusion liquid is high, and wherein total nitrogen is up to 559.4-704 mg/L, belongs to difficulty
Handle waste water.By microorganism decomposition and process of enriching treated supernatant, total nitrogen content is dropped from 559.4-704 mg/L
Down to 53 mg/L, total phosphorus is reduced to 5.6-7.2 mg/L from 31.9-40.1 mg/L, illustrates that microbial bacterial agent has efficiently played wadding
Solidifying and enrichment, 92% N element and 82% P element are enriched in rich feeding sludge after processing, and the N element in the feeding sludge of richness is high
Up to 5800 mg/L, P element is up to 544mg/L.
Experimental data in table 3 shows, the extrusion liquid indices after processing that undergo microbial fermentation decline, and stink obviously subtracts
Few, the supernatant after precipitating reaches State Ministry of Environmental Protection " livestock culture industry emission standard GB18596-2001 " defined collection
Reduction livestock culture industry water pollutant highest allows the discharge standard of average daily concentration of emission requirement.
Embodiment 3
The richness precipitated in embodiment 2 is supported sludge to rush excrement with 1 solid cow dung layer of embodiment and water and mix, adjusts above-mentioned mixing
The moisture content of object is adjusted to 60%, and bacillus subtilis and Paenibacillus polymyxa 1:1 Mixed Microbes is added according to volume ratio 1:1000
Liquid stirs evenly post-fermentation, and the cool fermentation 10d of natural heap after 70 DEG C of fermentation 8d, gained tunning is fertilizer I.
The total nutrient content in fertilizer I is measured, the experimental results showed that, the total nutrient in produced fertilizer I reaches 4.2-
5.3%, meet Minister Agriculture of China " organic fertilizer NY 525-2012 standard ".
Embodiment 4
The richness precipitated in embodiment 2 is supported sludge to rush excrement with 1 solid cow dung layer of embodiment and water and mix, adjusts above-mentioned mixing
The moisture content of object is adjusted to 60%, and bacillus subtilis and Paenibacillus polymyxa 1:1 Mixed Microbes is added according to volume ratio 1:1000
Liquid stirs evenly post-fermentation, and the cool fermentation 10d of natural heap after 80 DEG C of fermentation 6d, gained tunning is fertilizer II.
The total nutrient content in fertilizer II is measured, the experimental results showed that, the total nutrient in produced fertilizer II reaches 4.2-
5.3%, meet Minister Agriculture of China " organic fertilizer NY 525-2012 standard ".
Embodiment 5
The content of beary metal of products therefrom or product in the various embodiments described above is detected, NY525-2012 national standard, detection are contrasted
The product of each processing stage or the heavy metal of product situation up to standard, experimental result is shown in the following table 4.
Content of beary metal (unit: mg/kg) and situation up to standard in each operation stage of table 4.
The experimental results showed that the content of beary metal of product or product that the various embodiments described above obtain meets national standard, produced
Raw organic fertilizer also can achieve the international requirement to the content of beary metal in organic fertilizer.
Embodiment 5
The fertilizer 1kg produced under the conditions of different fermentations is taken, is mixed with the clean sandy soil 9kg to sterilize under the conditions of 120 DEG C of 30min
It closes, spreading for cooling spreads suitable quantity of water in flat culture vessel, and illumination cultivation in 22-25 DEG C of greenhouse measures the weed seed in fertilizer
As a result germination percentage see the table below 5.
Weed seed germination percentage in fertilizer under the conditions of 5. different fermentations of table
Above the experimental results showed that, when fermentation temperature is that 70 DEG C of fermentation times are 8d or fermentation temperature be 80 DEG C of fermentation times is 6d
When, the germination percentage of the weed seed in fertilizer is 0%, and weed seed can be killed completely, which, which efficiently solves, applies
The problem of causing weeds in field to be grown thickly with traditional organic fertilizer, ensure that present invention fertilizer produced is widely popularized application.
Embodiment 6
Using pure water as control, the GI index of cucumber and radish seed in the case where the fertilizer I that embodiment 3 produces acts on, tool are measured
Gymnastics is made as follows: weighing I 10.0g of fertilizer of the production of embodiment 3, is placed in 250mL conical flask, by solid-to-liquid ratio (mass/volume)
The deionized water or distilled water of 100mL is added in 1:10, is perpendicularly fixed on reciprocating horizontal vibrating machine after covering tightly bottle cap, adjusts frequency
Rate be not less than 100 times/min, amplitude be not less than 40mm, at room temperature oscillation extraction 1h, remove stand 0.5h after, take supernatant in
It installs and is filtered on the filter device of filter paper in advance, collect filtered leaching liquor, shake up rear spare.It is padded in 9cm culture dish
Upper two filter paper, is uniformly put into cucumber (or radish) seed that 10 sizes are almost the same, full, be added I leaching liquor of fertilizer (or
Pure water) 5mL, ware lid is covered, culture 48h, statistics germination percentage and measurement root long are protected from light in 25 DEG C of incubator, calculates seed
Germination index GI, the results are shown in Table 6:
Seed germination index (GI) calculation formula: GI=(A1 × A2)/(B1 × B2)
In formula:
The percentage of seedgermination of I leaching liquor of A1-fertilizer, %；
The average root long of I leaching liquor culture seed of A2-fertilizer, mm；
B1-pure water percentage of seedgermination, %；
The average root long of the pure Aquaponic seed of B2-, mm.
Influence of 6 fertilizer I of table to percentage of seedgermination
The results showed that the fertilizer I that embodiment 3 produces is 115% to the GI index of cucumber, the GI index to radish is 108%,
2 kinds of plant growths can be remarkably promoted using the fertilizer that the method provided by the present invention is fermented.
Claims (7)
1. a breeding class feces of farm recycling bioremediation, it is characterised in that described method includes following steps:
(1) excrement and liquid dung mixed liquor are drained into sedimentation basin natural sedimentation, the percolate after collecting diafiltration, sediment is layered shape
At 2 layers, upper layer is solid cow dung layer, and lower layer is sandy soils；
(2) percolate is separated into water by solid-liquid separating machine and rushes excrement and extrusion liquid；
(3) extrusion liquid is discharged into anaerobic fermentation in the anaerobic pond of the active sludge in bottom, 36 DEG C of fermentation temperature, residence time
12h, muddy water ratio 1:3, dissolved oxygen levels are not more than 0.2mg/L；
(4) supernatant liquor for completing anaerobic fermentation is subjected to aerobic fermentation, microbial bacterial agent is added in 1:1000 by volume, described
Microbial bacterial agent is the mix bacterium agent of Paenibacillus polymyxa and streptococcus equi, 28 DEG C of fermentation temperature, residence time 12h, is kept
Dissolved oxygen levels are higher than 2mg/L；
(5) mixture is imported into sedimentation basin, natural sedimentation 12h, supernatant can discharge, the solid cow dung layer of precipitating and step (1)
Excrement mixing is rushed with the water of step (2)；
(6) moisture content of mixture is adjusted to 60%, bacillus subtilis and mostly viscous class gemma bar is added according to volume ratio 1:1000
Bacterium 1:1 mixed bacteria liquid, the cool fermentation 10d of natural heap after 70 DEG C of fermentation 8d, gained tunning is fertilizer.
2. domestic animals feces of farm recycling bioremediation according to claim 1, it is characterised in that the step
(2) solid-liquid separating machine in is screw extruding type solid-liquid separator.
3. domestic animals feces of farm recycling bioremediation according to claim 1, it is characterised in that the step
(2) water content that the water in rushes excrement is 65-75%.
4. domestic animals feces of farm recycling bioremediation according to claim 1, it is characterised in that the step
(4) microbial inoculum in is that 3:1 is mixed to prepare by volume for the bacterium solution of Paenibacillus polymyxa and streptococcus equi.
5. domestic animals feces of farm recycling bioremediation according to claim 1, it is characterised in that the step
(6) fermentation condition in can be the cool fermentation 10d of natural heap after 80 DEG C of fermentation 6d.
6. domestic animals feces of farm recycling bioremediation according to claim 1, it is characterised in that the step
(4) Paenibacillus polymyxa in is JP-15, and the submitted national common micro-organisms collection of the bacterial strain carries out patent guarantor
Hiding, culture presevation number: CGMCC No.17230.
7. domestic animals feces of farm recycling bioremediation according to claim 1, it is characterised in that the step
(4) streptococcus equi in is JP-9, and the submitted national common micro-organisms collection of the bacterial strain carries out Patent Deposit, and strain is protected
Hiding number: CGMCC No.17231.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111172076A (en) * | 2019-12-17 | 2020-05-19 | 中国科学院上海高等研究院 | Bacterial strain for treating feces in ecological toilet and application thereof |
| CN112375693A (en) * | 2020-11-19 | 2021-02-19 | 陕西麦可罗生物科技有限公司 | Method for preparing microbial agent by utilizing natural bioflocculant to pretreat kasugamycin fermentation hyphae |
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| CN111172076A (en) * | 2019-12-17 | 2020-05-19 | 中国科学院上海高等研究院 | Bacterial strain for treating feces in ecological toilet and application thereof |
| CN111172076B (en) * | 2019-12-17 | 2020-10-16 | 中国科学院上海高等研究院 | Bacterial strain for treating feces in ecological toilet and application thereof |
| CN112375693A (en) * | 2020-11-19 | 2021-02-19 | 陕西麦可罗生物科技有限公司 | Method for preparing microbial agent by utilizing natural bioflocculant to pretreat kasugamycin fermentation hyphae |
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Application publication date: 20191022 |