CN110256608A - The method that horseradish peroxidase processing removes lignin in plant fiber pre-hydrolyzed solution - Google Patents
The method that horseradish peroxidase processing removes lignin in plant fiber pre-hydrolyzed solution Download PDFInfo
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Abstract
The invention discloses the methods that a kind of processing of horseradish peroxidase removes lignin in plant fiber pre-hydrolyzed solution, belong to plant fibre components and isolate and purify field.This method comprises: S1. carries out probe type ultrasonic processing to plant fiber material pre-hydrolyzed solution；S2. soluble calcium salt is added in pre-hydrolyzed solution after ultrasound exposure, is then handled again using horseradish peroxidase；S3. treated, and hydrolyzate goes to obtain pre-hydrolyzed solution after purification after precipitating.Compared with prior art, the method for the present invention can significantly improve the removal rate of lignin using the horseradish peroxidase processing combination in the presence of probe type ultrasonic processing and calcium ion, and the loss of oligosaccharide is seldom, has good application value.
Description
Technical field
The present invention relates to plant fibre components to isolate and purify field, specifically provides a kind of horseradish peroxidase processing removing
The method of lignin in plant fiber pre-hydrolyzed solution.
Background technique
From hydrolysis, prehydrolysis, the mistake being exactly hydrolyzed in plant fiber material hydrolytic process only with hot water are also
Journey.Deacetylation occurs in the acetyl group from hydrolytic process in fibrous raw material and generates acetic acid, acetic acid can be catalyzed hemicellulose
Hydrolysis and dissolution, therefore be considered as the effective ways and sulfuric acid of a kind of environmental-friendly extraction hemicellulose from hydrolysis
Method produces the important method that dissolving pulp removes hemicellulose from raw material in the process.From in hydrolytic process, in fibrous raw material
Hemicellulose is dissolved into pre-hydrolyzed solution.The hemicellulose being dissolved into pre-hydrolyzed solution exists in the form of oligosaccharide mostly, few
Amount exists in the form of monosaccharide.These oligosaccharide have a critically important application value in food, medicine and chemical industry, in hydrolyzate
Sugar can also be used to carry out fermenting and producing fuel such as ethyl alcohol etc..In pre-hydrolysis process, also have part lignin be dissolved in it is pre-
In hydrolyzate, also containing small molecular weight impurities such as furfural, hydroxymethylfurfural and acetic acid in pre-hydrolyzed solution.In order to pre- using fibrous raw material
Hemicellulose sugar in hydrolyzate, needs to isolate and purify pre-hydrolyzed solution.Film filtering is that removing hydrolyzate small molecular is miscellaneous
The matter such as important method of acetic acid, furfural and the important step of concentrating hydrolysate.But the lignin in pre-hydrolyzed solution will lead to film
Blocking, the production efficiency for filtering film reduce, while also leading to the shortening for filtering membrane lifetime.In addition, using in pre-hydrolyzed solution
Hemicellulose sugar fermenting and producing fuel when, the lignin in hydrolyzate is also harmful to enzymatic hydrolysis and fermentation process.Therefore, pre- water is removed
Major impurity-lignin in liquid is solved, hemicellulose sugar in pre-hydrolyzed solution is isolated and purified and using there is important meaning.
Have many methods at present and remove lignin therein from hydrolyzate, these methods include flocculation, absorption, extraction,
The methods of acidification.Flocculation includes cation such as polyacrylamide, diallyl dimethyl ammoniumchloride using high molecular polymer
With non-ionic polymers such as polyethylene glycol oxide flocculation treatment；Absorption method is using active carbon, quick lime, zeolite and macroreticular resin etc..
In addition, there are also the sides for removing lignin in hydrolyzate using the methods of cellulase treatment or cellulase treatment combination cation flocculation
Method.In order to reach high removal rate, in practice generally using a variety of methods in conjunction with technique remove the wood in pre-hydrolyzed solution
Element.
It discloses there are many technology from plant fiber material hydrolyzate at present and removes lignin, e.g., Chinese patent literature
201610865111.8 disclose and a kind of remove hydrolyzate using pectin enzyme pretreatment combination cationic polymer flocculation treatment
The method of middle lignin.Method includes the following steps: (1) pectase is added into wood fibre hydrolysis liquid carries out enzymatic treatment, fruit
Glue enzyme dosage is 20-1000g/ tons of hydrolyzates, 20 DEG C -70 DEG C of enzymatic treatment temperature, handles time 1h-48h, pH 3.0-
8.0, obtain hydrolyzate after enzymatic treatment；(2) hydrolyzate is handled using cationic flocculant after enzymatic treatment, treated hydrolyzate
Hydrolyzate after purification is obtained after standing or being centrifuged off precipitating.
Chinese patent literature 2017111938442 discloses a kind of using horseradish peroxidase combination cationic polymer
The method that flocculation treatment removes lignin in lignocellulosic hydrolysate changes method the following steps are included: (1) is to lignocellulosic hydrolysate
Middle addition horseradish peroxidase carries out enzymatic treatment, and horseradish peroxidase dosage is 5000-20000U/L hydrolyzate, peroxidating
Hydrogen dosage is 4.0-12g/L hydrolyzate, 15 DEG C -45 DEG C of enzymatic treatment temperature, time 4h-12h, pH 3.5-7.0, after enzymatic treatment
Precipitating is removed, hydrolyzate after enzymatic treatment is obtained；(2) cationic flocculant diallyl dimethyl is added to hydrolyzate after enzymatic treatment
One of ammonium chloride, polyethyleneimine and polyamine or in which two kinds of mixture are handled, and are then allowed to stand or are centrifuged off
Precipitate to obtain hydrolyzate after purification.
Chinese patent literature 201811148745.7 discloses a kind of hydrolyzate extraction for producing and generating during dissolving pulp
The method of xylose and lignin.Method includes the following steps: the extraction of (1) hydrolyzate；(2) hydrolyzate is neutralized with calcium carbonate
It is 2.8-3.2 to pH, then plus active carbon decoloring is to light transmission 30%, is exchanged by cation-anion resin, hydrolyzate pH is adjusted
To 4.0-7.0, hydrolyzate refractive power is concentrated into 16-20 by being evaporated in vacuo, temperature adjustment is to 28-30 DEG C；(3) by the water of step (2)
Solution liquid filters to get filtrate and filter residue, filter residue dry to obtain lignin at 60-65 DEG C, and filtrate is decolourized de- with cation-anion resin
Color liquid；(4) decoloration by multiple-effect evaporation to refractive power 55-60, after be 80-84 by single effect evaporation to refractive power massecuite；(5) massecuite
It is crystallized by the way of ladder cooling；(6) further drying obtains the dry xylose of finished product to xylose crystals at 60-70 DEG C.
The ultrasonic wave that Chinese patent literature 201810299860.8 discloses xylose in a kind of Eucalyptus hot water pre-hydrolyzed solution is auxiliary
Purification process is helped, which is using the processing of calcium hydroxide synergistic supersonic wave, two sections of activated carbon adsorptions and laccase collaboration
The method that physics, chemistry, the biofacies of ultrasonication combine, handles Eucalyptus hydro-thermal pre-hydrolyzed solution, excess calcium hydroxide particle
Suction-operated, dissolved organic matters such as lignin, furfural in separation removal pre-hydrolyzed solution except xylose and miscellaneous to the maximum extent
Matter improves the purity of xylose in pre-hydrolyzed solution.
Chinese patent literature 201310554290.X, which is disclosed, a kind of extracts oligosaccharide from the plant material pre-hydrolyzed solution
Method, steps are as follows: (1) polyelectrolyte being added into plant material pre-hydrolyzed solution, the polyelectrolyte is polyaluminium chloride, gathers
One of aluminum sulfate, polychloride ferro-aluminum, poly aluminium sulfate iron, poly aluminium iron silicate, poly-iron chloride, polyiron sulfate or combination, polyelectrolyte
Additional amount is 0.01-10g/L, is stirred 5-20 minutes, stands 80-150 minutes, isolated supernatant liquor；(2) with UF membrane
Method filters supernatant liquor, obtains oligosaccharide concentrate；(3) evaporative crystallization oligosaccharide concentrate obtains oligosaccharide after drying.
Lignin is separated off from hydrolyzate at present, in the various technologies for improving hemicellulose sugar purity, single processing
Method lignin removal rate is lower, therefore the technique generally combined using two or more methods.Contain cationic polymerization using
Object flocculation treatment or acidification such as sulfuric acid treatment processing remove in pre-hydrolyzed solution in the process of lignin, and cationic polymer or acid are to ring
Border and subsequent processing have potential adverse effect.It the methods of is adsorbed using active carbon and exchanger resin it is necessary to be carried out to adsorbent
Regeneration may lead to the problem of secondary pollution in regenerative process.In addition, processing step is more, operation is more cumbersome, and water
It is also more to solve loss sugared in liquid.
Problem existing for impurity: single minimizing technology impurity-eliminating effect is removed for existing plant fiber material pre-hydrolyzed solution
Difference, cannot effectively remove lignin, still there is impurity residual；When using a variety of method combination processings, although lignin removal increases
Add, but the loss amount of sugar is big, that is, lignin removal selectivity is poor, operation also becomes cumbersome, and it is possible to introduce other
There is the impurity of potential hazard.Therefore, it is necessary to research and develop, a kind of lignin removal rate is high, and easy to operate, sugared loss late is low and environmentally friendly
Processing method.Have great importance for the purifying of hemicellulose sugar and later use in pre-hydrolyzed solution.
Summary of the invention
During extracting oligosaccharide the present invention be directed to existing plant fiber material hydrolyzate, major impurity lignin removal effect
Rate is lower and poor selectivity, the big problem of oligosaccharide loss late, provides that a kind of lignin removal rate is high, few peppery of the loss of oligosaccharide
The method that root peroxide enzymatic treatment removes lignin in plant fiber pre-hydrolyzed solution.
Plant fiber pre-hydrolyzed solution of the present invention is obtained to plant fiber material progress from hydrolysis process with hot water
Pre-hydrolyzed solution.The plant fiber material can be leaf wood and bamboo, wheat straw, straw, one in reed non-wood material
Kind.It is 140-190 DEG C from hydrolysis process temperature, is 30-180min, the mass ratio of water and plant fiber material from hydrolysis time
Are as follows: 4:1-20:1.
The technical solution adopted by the present invention to solve the technical problems is: horseradish peroxidase processing removes plant fiber
The method of lignin in pre-hydrolyzed solution, comprising:
S1. probe type ultrasonic processing is carried out to plant fiber material pre-hydrolyzed solution；
S2. soluble calcium salt is added in pre-hydrolyzed solution after ultrasound exposure, then uses horseradish peroxidase and mistake again
Hydrogen oxide carries out enzymatic treatment；
S3. treated, and hydrolyzate goes to obtain pre-hydrolyzed solution after purification after precipitating.
In the above method, the lignin in pre-hydrolyzed solution first passes through high-intensitive probe type ultrasonic processing, ultrasonication
Middle generation radical reaction, generates more phenolic hydroxyl groups and alcoholic extract hydroxyl group.Pre-hydrolyzed solution after ultrasonic treatment carries out continuous horseradish peroxide
Compound enzymatic treatment, in the presence of hydrogen peroxide, on the one hand, phenolic hydroxyl group causes benzene oxygen radical in horseradish peroxidase enzyme catalytic,
And then polymerization occurs for lignin, and Lignin Molecular Weight increase is made to become insoluble lignin and be precipitated；On the other hand, horseradish peroxidase
Enzyme will lead to the oxidative degradation for also resulting in lignin, and in oxidation process, alcoholic extract hydroxyl group and phenolic hydroxyl group can be also oxidized in carboxyl,
In the presence of calcium ion, carboxyl and calcium ion in lignin form compound, become insoluble lignin and are precipitated, and then effectively improve
The removal rate of lignin, and reduce the loss of oligosaccharide.
In order to further enhance the sugared content of hydrolyzate after processing, pre-hydrolyzed solution after purification that step S3 is obtained can also be through
Anion-exchange resin column processing is crossed, obtains lignin removal rate greater than 94%, sugared purity is greater than 95% high-purity pre-hydrolyzed solution.
The processing time that probe type ultrasonic is handled in step S1 is preferably 25-90 minutes, and the optimization process time is 30-60 points
Clock；Temperature is preferably 10-45 DEG C, and most preferably 20-40 DEG C；Power is preferably 150-400W, most preferably 200W-300W；Prehydrolysis
The preferred pH of liquid is 3.0-9.0, most preferably pH4.0-8.0.
In step S2, the soluble calcium salt is preferably calcium nitrate and/or calcium chloride, in terms of calcium ion, calcium ion concentration
Preferably 0.5-2.5g/L pre-hydrolyzed solution, most preferably 1.0-2.0g/L pre-hydrolyzed solution.
Horseradish peroxidase dosage is preferably 1000-5000U/L pre-hydrolyzed solution, most preferably 1000-4000U/L prehydrolysis
Liquid.
Hydrogen peroxide use is preferably 0.5-5g/L pre-hydrolyzed solution, most preferably 1.0-3.0g/L pre-hydrolyzed solution.
Enzymatic treatment temperature is preferably 15 DEG C -45 DEG C, most preferably 20-40 DEG C in step S2；Time is preferably 2-12h, most
Good is 4-10h；Preferably pH 3.5-7.0, most preferably pH5.0-7.0.
In step S3, after treated hydrolyzate stands 4-12 hour or is centrifuged under 2000-3000 revs/min and goes precipitating
Obtain pre-hydrolyzed solution after purification.
Compared to the prior art, horseradish peroxidase of the invention processing removes lignin in plant fiber pre-hydrolyzed solution
Method have it is following prominent the utility model has the advantages that
Firstly, ultrasonic treatment provides more phenolic hydroxyl groups and alcoholic extract hydroxyl group for the processing of subsequent horseradish peroxidase, have
Conducive to the progress of lignin polymerization reaction and oxidation reaction, so as to improve enzymatic treatment effect.And calcium ion can be with during enzymatic treatment
Insoluble lignin compound is formed with the lignin with carboxyl of generation at any time and is precipitated, and the progress of reaction is promoted.Therefore, should
Inventive method can significantly improve the removal rate of lignin, and the loss of oligosaccharide is seldom.By the method for the present invention preliminary treatment
Afterwards, the removal rate of lignin is up to 70-85% in plant fiber material pre-hydrolyzed solution, and sugared loss only has 6-9%.Using into one
After the anion-exchange resin column processing of step, the removal rate of lignin is up to 94% or more, and sugared purity is up to 95% or more.
Secondly, the method that the method for the present invention uses ultrasonic physics processing method combination cellulase treatment, the substance of introducing
There are calcium ion and minimal amount of horseradish peroxidase, the hydrogen peroxide of addition can consume substantially after the reaction.Therefore, this hair
The a small amount of substance introduced in bright method flow will not generate harmful influence to environment and subsequent processing, and processing only needs ultrasound
Processing and two step of enzymatic treatment are a kind of environmentally friendly and simple process flows.
Specific embodiment
The present invention is further explained in the light of specific embodiments, but not as a limitation of the invention.
[term explanation]
Total reducing sugar loss late: the percentage of total reducing sugar reduction amount and total reducing sugar quality in hydrolyzate in pre-hydrolyzed solution treatment process, with
Percentage indicates.
Lignin removal rate: refer to the percentage of Mass lost after lignin is before treatment in pre-hydrolyzed solution.
Furfural, hydroxymethylfurfural total removal rate: refer to that the gross mass of furfural and hydroxymethylfurfural is before treatment in pre-hydrolyzed solution
The percentage of reduction afterwards.
Sugared purity in pre-hydrolyzed solution: total material quality than water in the quality and pre-hydrolyzed solution of total reducing sugar in pre-hydrolyzed solution
Ratio.
Horseradish peroxidase enzyme activity: pyrogallic acid is catalyzed at 20 DEG C, pH 6.0, in 20 seconds and generates 1.0mg's
The enzyme amount of purpurogallin is an enzyme-activity unit.
Unless otherwise instructed, the content of following each ingredients used is mass percentage content.
[embodiment 1]
The method that horseradish peroxidase processing removes lignin in plant fiber pre-hydrolyzed solution:
The plant fiber pre-hydrolyzed solution is pre-hydrolyzed solution of the poplar from after hydrolyzing, total sugar content 14.5g/L, lignin content
5.9g/L。
Step process:
(1) it is handled using probe type ultrasonic (ultrasonic cell disruptor, model SEIENTZ- II), treatment conditions: processing
Time 45 minutes, 30 DEG C of temperature, power 200W, pH 6.0；
(2) calcium chloride is added into the processed pre-hydrolyzed solution of step 1, at horseradish peroxidase and hydrogen peroxide
Reason, calcium chloride dosage 4.0g/L, enzymatic treatment condition are as follows: enzyme dosage 1500U/L, H2O2Dosage 2.0g/L, 30 DEG C of temperature, time 6h,
pH 6.0。
(3) step 2 treated hydrolyzate stands 10h, and decantation removes precipitating, obtains upper layer pre-hydrolyzed solution after purification.
Pre-hydrolyzed solution after processing is detected, lignin removal rate is 85%, and total reducing sugar loss late is 7.5%.
[reference examples 1]
Processing method with embodiment 1 method, the difference is that: the reference examples without step (1) probe type ultrasonic at
Reason, directly the pre-hydrolyzed solution by poplar from after hydrolyzing carries out step (2) (3) processing, and is added without calcium ion during processing.
Through detecting, after the processing of reference examples 1, lignin removal rate is 35%, and total reducing sugar loss late is 5.2% in pre-hydrolyzed solution.
[reference examples 2]
Processing method with embodiment 1 method, the difference is that: the reference examples without step (1) probe type ultrasonic at
Reason, the directly pre-hydrolyzed solution by poplar from after hydrolyzing carry out step (2) (3) processing.
Through detecting, after the processing of reference examples 2, lignin removal rate is 66%, and pre-hydrolyzed solution total reducing sugar loss late is 7.0%.
[embodiment 2]
The method that horseradish peroxidase processing removes lignin in plant fiber pre-hydrolyzed solution:
The plant fiber pre-hydrolyzed solution is pre-hydrolyzed solution of the Eucalyptus from after hydrolyzing, total sugar content 13.2g/L, lignin content
5.3g/L。
Processing step:
(1) pre-hydrolyzed solution uses probe type ultrasonic (ultrasonic cell disruptor, model SEIENTZ- II) to handle first, place
Manage bar part: the processing time 30 minutes, 20 DEG C of temperature, power 150W, pH 7.0；
(2) after pre-hydrolyzed solution ultrasonic treatment, calcium chloride is added, using horseradish peroxidase and hydrogen peroxide treatment, chlorine
Change calcium dosage 5.0g/L, enzymatic treatment condition are as follows: enzyme dosage 2000U/L, H2O2Dosage 2.0g/L, 25 DEG C of temperature, time 8h, pH
6.5。
(3) horseradish peroxidase treated hydrolyzate stands 10h, and decantation removes precipitating, it is pre- after purification to obtain upper layer
Hydrolyzate.
Pre-hydrolyzed solution after processing is detected, lignin removal rate 83%, total reducing sugar loss late 8.0%.
[reference examples 3]
Processing method with embodiment 2 method, the difference is that: this method without step (1) probe type ultrasonic handle,
The pre-hydrolyzed solution from after hydrolyzing directly is subjected to step (2) (3) processing, and is added without calcium ion during processing.
Through detecting, after the processing of reference examples 3, lignin removal rate is 32%, and total reducing sugar loss late is 5.2% in pre-hydrolyzed solution.
[reference examples 4]
Processing method with embodiment 2 method, the difference is that: this method without step (1) probe type ultrasonic handle,
The pre-hydrolyzed solution from after hydrolyzing directly is subjected to step (2) (3) processing.
Through detecting, after the processing of reference examples 4, lignin removal rate is 66%, and total reducing sugar loss late is 7.0% in pre-hydrolyzed solution.
[embodiment 3]
The method that horseradish peroxidase processing removes lignin in plant fiber pre-hydrolyzed solution:
The plant fiber pre-hydrolyzed solution is pre-hydrolyzed solution of the wheat straw from after hydrolyzing, total sugar content 14.2g/L, lignin content
5.6g/L。
Steps are as follows:
(1) pre-hydrolyzed solution uses probe type ultrasonic (ultrasonic cell disruptor, model SEIENTZ- II) to handle first, place
Manage bar part: the processing time 45 minutes, 25 DEG C of temperature, power 250W, pH 6.0；
(2) after pre-hydrolyzed solution ultrasonic treatment, calcium nitrate is added, using horseradish peroxidase and hydrogen peroxide treatment, nitre
Sour calcium dosage 5.0g/L, enzymatic treatment condition are as follows: enzyme dosage 2500U/L, H2O2Dosage 2.5g/L, 20 DEG C of temperature, time 8h, pH
6.0。
(3) horseradish peroxidase treated hydrolyzate stands 10h, and decantation removes precipitating, it is pre- after purification to obtain upper layer
Hydrolyzate.
Pre-hydrolyzed solution after processing is detected, lignin removal rate 80%, total reducing sugar loss late 8.5%.
[reference examples 5]
Processing method with embodiment 3 method, the difference is that: this method without step (1) probe type ultrasonic handle,
The pre-hydrolyzed solution from after hydrolyzing directly is subjected to step (2) (3) processing, and is added without calcium ion during processing.
After the processing of reference examples 5, lignin removal rate is 35%, and total reducing sugar loss late is 5.0% in pre-hydrolyzed solution.
[reference examples 6]
Processing method with embodiment 3 method, the difference is that: this method without step (1) probe type ultrasonic handle,
The pre-hydrolyzed solution from after hydrolyzing directly is subjected to step (2) (3) processing.
After the processing of reference examples 6, lignin removal rate is 71%, and total reducing sugar loss late is 7.3% in pre-hydrolyzed solution.
[embodiment 4]
The resulting pre-hydrolyzed solution of embodiment 1 is handled using anion-exchange resin column, the prehydrolysis after being purified
Liquid.
After above-mentioned steps are handled, the total removal rate of lignin reaches 95.0% in pre-hydrolyzed solution, while by the above step
After rapid processing, impurity furfural, hydroxymethylfurfural total removal rate 95.2% in pre-hydrolyzed solution finally obtain sugared purity in pre-hydrolyzed solution
It is 97.1%, the loss of total reducing sugar is 9.5%.
[embodiment 5]
It will handle such as the resulting pre-hydrolyzed solution of embodiment 2 using anion-exchange resin column, the pre- water after being purified
Liquid is solved, after above-mentioned steps are handled, the total removal rate of lignin reaches 94.2% in pre-hydrolyzed solution, while by above step
After reason, furfural, hydroxymethylfurfural total removal rate 94.8% finally obtain after purification that sugared purity is 95.4% in pre-hydrolyzed solution, always
The loss of sugar is 9.8%.
[embodiment 6]
With the method that horseradish peroxidase described in embodiment 1 processing removes lignin in plant fiber pre-hydrolyzed solution, institute
The difference is that the temperature of the probe type ultrasonic processing used is 20 DEG C.
Pre-hydrolyzed solution after processing is detected, lignin removal rate 82%, total reducing sugar loss late 7.4%.
[embodiment 7]
With the method that horseradish peroxidase described in embodiment 1 processing removes lignin in plant fiber pre-hydrolyzed solution, institute
The difference is that the dosage of hydrogen peroxide is 1.0g/L when horseradish peroxidase processing.
Pre-hydrolyzed solution after processing is detected, lignin removal rate 78%, total reducing sugar loss late 7.2%.
[embodiment 8]
With the method that horseradish peroxidase as described in example 2 processing removes lignin in plant fiber pre-hydrolyzed solution, institute
The difference is that enzyme dosage used when horseradish peroxidase processing is 3000U/L, hydrogen peroxide use 1.5g/L.
Pre-hydrolyzed solution after processing is detected, lignin removal rate 81%, total reducing sugar loss late 7.8%.
[embodiment 9]
With the method that horseradish peroxidase described in embodiment 1 processing removes lignin in plant fiber pre-hydrolyzed solution, institute
The difference is that the pre-hydrolyzed solution handled is the hydrolyzate that bamboo is obtained from after hydrolyzing.
Total sugar concentration is 13.5g/L, lignin concentration 4.6g/L in untreated preceding pre-hydrolyzed solution.
It handles, pre-hydrolyzed solution after processing is detected, lignin removal rate is through method described in same embodiment 1
79%, total reducing sugar loss is 8.4%.
Claims (8)
1. the method that horseradish peroxidase processing removes lignin in plant fiber pre-hydrolyzed solution, characterized by comprising:
S1. probe type ultrasonic processing is carried out to plant fiber material pre-hydrolyzed solution；
S2. soluble calcium salt is added in pre-hydrolyzed solution after ultrasound exposure, then uses horseradish peroxidase and peroxidating again
Hydrogen carries out enzymatic treatment；
S3. treated, and hydrolyzate goes to obtain pre-hydrolyzed solution after purification after precipitating.
2. the method that horseradish peroxidase processing according to claim 1 removes lignin in plant fiber pre-hydrolyzed solution,
It is characterized by: the pre-hydrolyzed solution after purification that step S3 is obtained is handled by anion-exchange resin column, lignin removal rate is obtained
Greater than 94%, sugared purity is greater than 95% high-purity pre-hydrolyzed solution.
3. the side that horseradish peroxidase processing according to claim 1 or 2 removes lignin in plant fiber pre-hydrolyzed solution
Method, it is characterised in that:
The processing time that probe type ultrasonic is handled in step S1 is 25-90 minutes, and temperature is 10-45 DEG C, power 150-400W,
Pre-hydrolyzed solution pH is 3.0-9.0.
4. the side that horseradish peroxidase processing according to claim 1 or 2 removes lignin in plant fiber pre-hydrolyzed solution
Method, it is characterised in that in step S2, the soluble calcium salt is calcium nitrate and/or calcium chloride, in terms of calcium ion, calcium ion concentration
For 0.5-2.5g/L pre-hydrolyzed solution.
5. the side that horseradish peroxidase processing according to claim 1 or 2 removes lignin in plant fiber pre-hydrolyzed solution
Method, it is characterised in that in step S2, horseradish peroxidase dosage is 1000-5000U/L pre-hydrolyzed solution.
6. the side that horseradish peroxidase processing according to claim 1 or 2 removes lignin in plant fiber pre-hydrolyzed solution
Method, it is characterised in that in step S2, hydrogen peroxide use is 0.5-5g/L pre-hydrolyzed solution.
7. the side that horseradish peroxidase processing according to claim 1 or 2 removes lignin in plant fiber pre-hydrolyzed solution
Method, it is characterised in that in step S2,15 DEG C -45 DEG C of enzymatic treatment temperature, time 2-12h, pH 3.5-7.0.
8. the side that horseradish peroxidase processing according to claim 1 or 2 removes lignin in plant fiber pre-hydrolyzed solution
Method, it is characterised in that in step S3, treated hydrolyzate stands 4-12 hours or is centrifuged under 2000-3000 revs/min and goes
Pre-hydrolyzed solution after purification is obtained after precipitating.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111233252A (en) * | 2020-01-15 | 2020-06-05 | 北京建筑大学 | Zeolite imidazolate-like framework-horseradish peroxidase biomineralization embedding precoat, preparation thereof and application thereof in ultrafiltration membrane water purification |
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| CN111233252B (en) * | 2020-01-15 | 2021-08-13 | 北京建筑大学 | Zeolite imidazolate-like framework-horseradish peroxidase biomineralization embedding precoat, preparation thereof and application thereof in ultrafiltration membrane water purification |
| CN114113591A (en) * | 2021-11-17 | 2022-03-01 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Enzyme linked immunosorbent assay method for sensitively detecting Escherichia coli O157H7 |
| CN114113591B (en) * | 2021-11-17 | 2022-06-21 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Enzyme linked immunosorbent assay method for sensitively detecting Escherichia coli O157H7 |
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| WO2023143310A1 (en) * | 2022-01-25 | 2023-08-03 | 齐鲁工业大学 | Method for isolating saccharides in pre-hydrolysates of hardwood pulping |
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| CN114657807A (en) * | 2022-04-02 | 2022-06-24 | 山东太阳纸业股份有限公司 | Method for promoting lignin accumulation in wood pulping prehydrolysis liquid by using biological enzyme |
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