CN110237143A - A method of enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone - Google Patents
A method of enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone Download PDFInfo
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- CN110237143A CN110237143A CN201910515891.7A CN201910515891A CN110237143A CN 110237143 A CN110237143 A CN 110237143A CN 201910515891 A CN201910515891 A CN 201910515891A CN 110237143 A CN110237143 A CN 110237143A
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- flavone
- enzymatic hydrolysis
- chinese lizardtail
- lizardtail
- auxiliary reflux
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/78—Saururaceae (Lizard's-tail family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The present invention relates to saururus chinensis extractive technique fields, specially a kind of method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone, it is extracted by carrying out cellulase degradation auxiliary reflux method to saururus chinensis, and the cellulase dosage in extraction process, enzymatic hydrolysis pH value, hydrolysis temperature, the technological parameters such as enzymolysis time are optimized, so that Chinese lizardtail flavone yield reaches 7.80mg/g or more；Time-consuming is effectively reduced, while reducing the usage amount of organic solvent, has achieved the purpose that high efficiency extraction Chinese lizardtail flavone.
Description
Technical field
The present invention relates to saururus chinensis extractive technique field, specially a kind of enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone
Method.
Background technique
Saururus chinensis is the dry aerial parts of Saururaceae plant saururus chinensis Saururus chinensis (Lour.), medicine
Reason is research shows that saururus chinensis has anti-inflammatory, liver protection, hypoglycemic isoreactivity；Its chemical component rich in, such as volatile oil, Huang
Ketone, lignanoids etc.；Wherein, flavones ingredient is one of its effective component；For the extracting method of Chinese lizardtail flavone, mesh
It is preceding main using microwave method, circumfluence method, ultrasonic method, warm leaching method；Wherein, circumfluence method and warm leaching method are in the presence of time-consuming, solvent usage
The disadvantages of big, although and microwave method and ultrasonic method are time-consuming short, extraction effect is also undesirable, and to extract equipment require compared with
It is high.
Enzymatic Extraction is a biotechnology, and appropriate biological enzyme is selected according to medicinal material feature, can be mild
Under conditions of plant cell wall is degraded, promote the release of effective component, to reach high efficiency extraction effective component of chinese medicine or portion
The purpose of position；Enzymatic Extraction has many advantages, such as that extraction conditions are mild, energy consumption is small, to equipment without particular/special requirement, so that enzyme process and often
Flavones ingredient has obtained a large amount of research in rule extracting method combined extracting natural plants, but there is not yet enzyme process is mentioned with conventional
Method is taken to combine the application study in Chinese lizardtail flavone extraction.
Extraction cost is reduced, is originally ground in order to realize enzymatic hydrolysis auxiliary reflux high efficiency extraction Chinese lizardtail flavone based on this
The person of studying carefully extracts the enzymolysis process condition of Chinese lizardtail flavone using optimization of orthogonal test enzymatic hydrolysis auxiliary reflux, is Chinese lizardtail flavone
Extraction a kind of new approaches are provided.
Summary of the invention
To solve the above-mentioned technical problems in the prior art, it is white that the present invention provides a kind of enzymatic hydrolysis auxiliary reflux extraction three
The method of careless general flavone, comprising the following steps:
(1) by saururus chinensis drying, crushing, Chinese Lizardtail Rhizome Or Herb is obtained；
(2) Chinese Lizardtail Rhizome Or Herb is weighed, buffer solution is added, adjustment pH value is 4.0-6.0, according still further to quality of medicinal material than being added
0.4%-1.6% cellulase, hydrolysis temperature are 40-60 DEG C, digest 30-90min；
(3) material after step (2) enzymatic hydrolysis is filtered, 10 times of 70% ethyl alcohol of amount, refluxing extraction is added into filter residue
1h obtains crude extract；
(4) crude extract is concentrated into thick paste using Rotary Evaporators, and 12-30h is dried using vacuum oven.
Further, the Chinese Lizardtail Rhizome Or Herb is 20-80 mesh.
Further, the buffer solution is acetic acid-Acetate Solution, phosphoric acid-phosphate dihydrogen salt solution, phosphoric acid-phosphoric acid
It is any in hydrogen salt solution.
Further, the acetate be sodium acetate, potassium acetate, it is any in ammonium acetate.
Further, the dihydric phosphate be potassium dihydrogen phosphate, it is any in sodium dihydrogen phosphate.
Further, the hydrophosphate be potassium hydrogen phosphate, it is any in hydrophosphate.
Further, the cellulase dosage is 1.3%, and hydrolysis temperature is 50 DEG C, and enzymatic hydrolysis pH value is 4.5, when enzymatic hydrolysis
Between be 75min.
Further, the step (4) dries for 24 hours.
The present invention uses sodium nitrite-aluminum nitrate-sodium hydroxide determination of color general flavone content, specifically includes following
Step:
In UV-VIS spectrophotometry continuous mode, the rutin stock solution 1.0 of precision measurement 0.215mg/mL,
2.0,3.0,4.0,5.0,6.0mL, are respectively placed in 25mL volumetric flask, respectively plus water 6.0mL, add 5% sodium nitrite solution 1mL,
After placing 6min, add 10% aluminum nitrate solution 1mL, after placing 6min, adding sodium hydroxide solution 10mL is added water to scale, put
After setting 15min, trap is measured at 500nm；And using trap as ordinate, using the concentration for measuring solution as abscissa, draw
The standard curve of Chinese lizardtail flavone processed, it is as follows to obtain regression equation:
YGeneral flavone=0.0073X+0.0318 (R2=0.9992), wherein YGeneral flavoneTo measure obtained absorbance value, X is measurement
The concentration of solution.
General flavone weight (mg)/saururus chinensis crude drug weight (g) that Chinese lizardtail flavone yield Y=is extracted；To fibre
Four factors that plain enzyme dosage, enzymatic hydrolysis pH value, hydrolysis temperature, enzymolysis time are influenced as Chinese lizardtail flavone yield are tieed up, and will
Aforementioned four factor carries out single factor experiment, determines optimal level of four factors under the premise of ceteris paribus, specific single
Factorial experiments operate following steps:
(1) cellulase dosage screening study:
To digest pH value 4.5,50 DEG C of hydrolysis temperature, enzymolysis time 60min, used according to quality of medicinal material than cellulase is added
Amount is respectively that 0.4%, 0.7%, 1.0%, 1.3%, 1.6% condition carries out enzymatic hydrolysis pretreatment, then carries out normal reflux extraction；
And tester calculates Chinese lizardtail flavone yield, measurement result is as shown in Figure 1, in enzymatic hydrolysis pH value, hydrolysis temperature, enzymolysis time
Under the same conditions, when enzyme dosage is 1.3%, general flavone yield highest.
(2) pH value screening study is digested:
With 50 DEG C, enzymolysis time 60min of cellulase dosage 1.3%, hydrolysis temperature, enzymatic hydrolysis pH value 4.0,4.5,5.0,
5.5,6.0 conditions carry out enzymatic hydrolysis pretreatment, then carry out normal reflux extraction；And tester calculates Chinese lizardtail flavone yield,
Its measurement result as shown in Fig. 2, cellulase dosage, hydrolysis temperature, enzymolysis time under the same conditions, when enzymatic hydrolysis pH value be
When 4.5, general flavone yield reaches maximum value.
(3) hydrolysis temperature screening study:
With cellulase dosage 1.3%, enzymatic hydrolysis pH value 4.5, enzymolysis time 60min, 40 DEG C of hydrolysis temperature, 45 DEG C, 50 DEG C,
55 DEG C, 60 DEG C of conditions carry out enzymatic hydrolysis pretreatment, then carry out normal reflux extraction；And tester calculates Chinese lizardtail flavone and obtains
Rate, measurement result is as shown in figure 3, in cellulase dosage, digest pH value, enzymolysis time under the same conditions, when enzymatic hydrolysis temperature
When degree is 50 DEG C, general flavone yield reaches maximum value.
(4) enzymolysis time screening study:
With cellulase dosage 1.3%, enzymatic hydrolysis pH value 4.5,50 DEG C of hydrolysis temperature, enzymolysis time 30min, 45min,
60min, 75min, 90min condition carry out enzymatic hydrolysis pretreatment, then carry out normal reflux extraction；And tester calculating saururus chinensis is total
Flavones yield, measurement result as shown in figure 4, cellulase dosage, enzymatic hydrolysis pH value, hydrolysis temperature under the same conditions, when
When enzymolysis time 60min, general flavone yield reaches maximum value.
After completing above-mentioned single factor experiment operation processing step, by the result that above-mentioned single factor experiment obtains draft for
Optimal level, and carry out the horizontal L of four factor three9(34) orthogonal test, experimental factor level is shown in Table 1:
Table 1
| Parameter | A enzyme dosage | B digests pH value | C hydrolysis temperature/DEG C | D enzymolysis time/min |
| 1 | 1.0% | 4.0 | 45 | 45 |
| 2 | 1.3% | 4.5 | 50 | 60 |
| 3 | 1.6% | 5.0 | 55 | 75 |
Orthogonal experiments are as shown in table 2:
Table 2
| Parameter | A enzyme dosage | B digests pH value | C hydrolysis temperature/DEG C | D enzymolysis time/min | General flavone yield mg/g |
| 1 | 1 | 1 | 1 | 1 | 7.80 |
| 2 | 1 | 2 | 2 | 2 | 11.98 |
| 3 | 1 | 3 | 3 | 3 | 11.32 |
| 4 | 2 | 1 | 2 | 3 | 10.75 |
| 5 | 2 | 2 | 3 | 1 | 12.23 |
| 6 | 2 | 3 | 1 | 2 | 10.41 |
| 7 | 3 | 1 | 3 | 2 | 8.27 |
| 8 | 3 | 2 | 1 | 3 | 12.45 |
| 9 | 3 | 3 | 2 | 1 | 9.49 |
| K1 | 10.367 | 8.940 | 10.220 | 9.840 | |
| K2 | 11.130 | 12.220 | 10.740 | 10.220 | |
| K3 | 10.070 | 10.407 | 10.607 | 11.507 | |
| R | 1.060 | 3.280 | 0.520 | 1.667 |
The results of analysis of variance is as shown in table 3:
Table 3
| Factor | Sum of square of deviations | Freedom degree | F ratio | F critical value | Conspicuousness |
| A enzyme dosage | 1.794 | 2 | 4.096 | 19.000 | |
| B digests pH value | 16.198 | 2 | 36.982 | 19.000 | * |
| C hydrolysis temperature/DEG C | 0.438 | 2 | 1.000 | 19.000 | |
| D enzymolysis time/min | 4.578 | 2 | 10.452 | 19.000 | |
| Error | 0.44 | 2 |
By above-mentioned orthogonal experiments it is found that enzymatic hydrolysis auxiliary reflux extracts the optimal enzymolysis process parameter of Chinese lizardtail flavone
Are as follows: enzyme dosage 1.3%, enzymatic hydrolysis pH value 4.5,50 DEG C of hydrolysis temperature, enzymolysis time 75min；It carries out at the process conditions three times
Parallel test, Chinese lizardtail flavone yield difference: 12.56mg/g, 12.38mg/g, 12.49mg/g, average value 12.48mg/g.
It is according to experimental result it is found that the process efficient, controllable, stable, feasible.
Beneficial effect
The invention is extracted by carrying out cellulase degradation auxiliary reflux method to saururus chinensis, and in extraction process
The technological parameters such as cellulase dosage, enzymatic hydrolysis pH value, hydrolysis temperature, enzymolysis time optimize, so that Chinese lizardtail flavone produces
Rate reaches 7.80mg/g or more；Time-consuming is effectively reduced, while reducing the usage amount of organic solvent, has reached high efficiency extraction saururus chinensis
The purpose of general flavone.
Detailed description of the invention
Fig. 1 is influence diagram of the enzyme dosage to Chinese lizardtail flavone yield.
Fig. 2 is to digest pH value to the influence diagram of Chinese lizardtail flavone yield.
Fig. 3 is influence diagram of the hydrolysis temperature to Chinese lizardtail flavone yield.
Fig. 4 is influence diagram of the enzymolysis time to Chinese lizardtail flavone yield.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
By saururus chinensis drying, 40 mesh are ground into, obtain Chinese Lizardtail Rhizome Or Herb；
Chinese Lizardtail Rhizome Or Herb 10.0000g is weighed, NaAc_HAc buffer solution is added, adjustment pH value is 4.5, according to medicinal material
1.0% cellulase is added in mass ratio, digests 60min at 50 DEG C；
Material after enzymatic hydrolysis is filtered, then by after enzymatic hydrolysis saururus chinensis medicinal material carry out normal reflux extraction (i.e. plus
Enter 10 times of 70% ethyl alcohol of amount, extract 1h) to get arriving crude extract；
Crude extract is concentrated into thick paste using Rotary Evaporators, and for 24 hours using vacuum oven drying, can be obtained and slightly mention
Object.
Weigh it is above-mentioned slightly take object, dissolved using anhydrous methanol, after dilution, using UV-VIS spectrophotometry measurement, meter
Calculate general flavone content；And general flavone weight (the mg)/saururus chinensis crude drug weight extracted according to Chinese lizardtail flavone yield Y=
It measures (g), calculating general flavone yield is 11.98mg/g.
Embodiment 2
By saururus chinensis drying, 40 mesh are ground into, obtain Chinese Lizardtail Rhizome Or Herb；
Chinese Lizardtail Rhizome Or Herb 10.0000g is weighed, NaAc_HAc buffer solution is added, adjustment pH value is 5.0, according to medicinal material
1.3% cellulase is added in mass ratio, digests 60min at 45 DEG C；
Material after enzymatic hydrolysis is filtered, then by after enzymatic hydrolysis saururus chinensis medicinal material carry out normal reflux extraction (i.e. plus
Enter 10 times of 70% ethyl alcohol of amount, extract 1h) to get arriving crude extract；
Crude extract is concentrated into thick paste using Rotary Evaporators, and for 24 hours using vacuum oven drying, can be obtained and slightly mention
Object.
Weigh it is above-mentioned slightly take object, dissolved using anhydrous methanol, after dilution, using UV-VIS spectrophotometry measurement, meter
Calculate general flavone content；And general flavone weight (the mg)/saururus chinensis crude drug weight extracted according to Chinese lizardtail flavone yield Y=
It measures (g), calculating general flavone yield is 10.41mg/g.
Embodiment 3
By saururus chinensis drying, 40 mesh are ground into, obtain Chinese Lizardtail Rhizome Or Herb；
Chinese Lizardtail Rhizome Or Herb 10.0000g is weighed, NaAc_HAc buffer solution is added, adjustment pH value is 4.5, according to medicinal material
1.6% cellulase is added in mass ratio, digests 75min at 45 DEG C；
Material after enzymatic hydrolysis is filtered, then by after enzymatic hydrolysis saururus chinensis medicinal material carry out normal reflux extraction (i.e. plus
Enter 10 times of 70% ethyl alcohol of amount, extract 1h) to get arriving crude extract；
Crude extract is concentrated into thick paste using Rotary Evaporators, and for 24 hours using vacuum oven drying, can be obtained and slightly mention
Object.
Weigh it is above-mentioned slightly take object, dissolved using anhydrous methanol, after dilution, using UV-VIS spectrophotometry measurement, meter
Calculate general flavone content；And general flavone weight (the mg)/saururus chinensis crude drug weight extracted according to Chinese lizardtail flavone yield Y=
It measures (g), calculating general flavone yield is 12.45mg/g.
Embodiment 4
By saururus chinensis drying, 40 mesh are ground into, obtain Chinese Lizardtail Rhizome Or Herb；
Chinese Lizardtail Rhizome Or Herb 10.0000g is weighed, NaAc_HAc buffer solution is added, adjustment pH value is 5.0, according to medicinal material
1.0% cellulase is added in mass ratio, digests 75min at 55 DEG C；
Material after enzymatic hydrolysis is filtered, then by after enzymatic hydrolysis saururus chinensis medicinal material carry out normal reflux extraction (i.e. plus
Enter 10 times of 70% ethyl alcohol of amount, extract 1h) to get arriving crude extract；
Crude extract is concentrated into thick paste using Rotary Evaporators, and for 24 hours using vacuum oven drying, can be obtained and slightly mention
Object.
Weigh it is above-mentioned slightly take object, dissolved using anhydrous methanol, after dilution, using UV-VIS spectrophotometry measurement, meter
Calculate general flavone content；And general flavone weight (the mg)/saururus chinensis crude drug weight extracted according to Chinese lizardtail flavone yield Y=
It measures (g), calculating general flavone yield is 11.32mg/g.
In some embodiments of the invention, for the powder particle size after drying and crushing between 20-80 mesh；?
Use buffer molten for acetic acid-Acetate Solution or phosphoric acid-phosphate dihydrogen salt solution or phosphoric acid-hydrophosphate in some embodiments
Liquid；The acetate is one of potassium acetate, ammonium acetate；The dihydric phosphate is potassium dihydrogen phosphate, biphosphate
One of sodium；The hydrophosphate is one of potassium hydrogen phosphate, hydrophosphate.
Test example 1
The present invention compares the extracting method of embodiment and existing microwave method, circumfluence method, ultrasonic method, warm leaching method；
Microwave method weighs Chinese Lizardtail Rhizome Or Herb 10.0000g, and in ethyl alcohol 70%, solid-liquid ratio 1:20, Extracting temperature is 60 DEG C of items
Microwave Extraction 240min under part；
Circumfluence method weighs Chinese Lizardtail Rhizome Or Herb 10.0000g, and in ethyl alcohol 70%, solid-liquid ratio 1:20, Extracting temperature is 60 DEG C of items
Refluxing extraction 240min under part；
Ultrasonic method weighs Chinese Lizardtail Rhizome Or Herb 10.0000g, is 65 DEG C of items in ethyl alcohol 50%, solid-liquid ratio 1:25, Extracting temperature
Ultrasonic extraction 30min under part；
Warm leaching method, weighs Chinese Lizardtail Rhizome Or Herb 10.0000g, and in ethyl alcohol 50%, solid-liquid ratio 1:30, Extracting temperature is 65 DEG C of items
Temperature leaching 240min under part；
As the result is shown such as the following table 4:
Table 4
| Group | Extraction time min | General flavone extracts yield mg/g |
| Embodiment 1 | 60 | 11.98 |
| Embodiment 2 | 60 | 10.41 |
| Embodiment 3 | 60 | 12.45 |
| Embodiment 4 | 60 | 11.32 |
| Microwave method | 240 | 11.63 |
| Circumfluence method | 240 | 11.84 |
| Ultrasonic method | 30 | 6.89 |
| Warm leaching method | 240 | 4.87 |
It is differed not it is found that extracting yield using microwave method and circumfluence method Chinese lizardtail flavone with the present invention by result in table 4
Obviously, but its extraction time is long, higher cost, and time-consuming for warm leaching method extraction, and Chinese lizardtail flavone extraction yield is lower, extracts effect
Fruit is poor；Ultrasonic extraction is used, although extraction time is short, it is not high to extract yield；Comprehensive analysis, using the present invention program couple
Chinese lizardtail flavone extracts；Comprehensive analysis is extracted using present invention enzymatic hydrolysis auxiliary reflux, and not only solvent usage is low, extraction time
Short, it is also more satisfactory that Chinese lizardtail flavone extracts yield.
Claims (8)
1. a kind of method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone, which comprises the following steps:
(1) by saururus chinensis drying, crushing, Chinese Lizardtail Rhizome Or Herb is obtained；
(2) Chinese Lizardtail Rhizome Or Herb is weighed, buffer solution is added, adjustment pH value is 4.0-6.0, according still further to quality of medicinal material than being added
0.4%-1.6% cellulase, hydrolysis temperature are 40-60 DEG C, digest 30-90min；
(3) material after step (2) enzymatic hydrolysis is filtered, 10 times of 70% ethyl alcohol of amount is added into filter residue, refluxing extraction 1h is obtained
To crude extract；
(4) crude extract is concentrated into thick paste using Rotary Evaporators, and 12-30h is dried using vacuum oven.
2. the method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone as described in claim 1, which is characterized in that described three are white
Grass meal end is 20-80 mesh.
3. the method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone as described in claim 1, which is characterized in that the buffering is molten
Liquid is acetic acid-Acetate Solution, phosphoric acid-phosphate dihydrogen salt solution, any in phosphoric acid-hydrophosphate solution.
4. the method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone as claimed in claim 3, which is characterized in that described
Acetate is sodium acetate, potassium acetate, any in ammonium acetate.
5. the method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone as claimed in claim 3, which is characterized in that the di(2-ethylhexyl)phosphate
Hydrogen salt is potassium dihydrogen phosphate, any in sodium dihydrogen phosphate.
6. the method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone as claimed in claim 3, which is characterized in that the phosphoric acid
Hydrogen salt is potassium hydrogen phosphate, any in hydrophosphate.
7. the method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone as described in claim 1, which is characterized in that the cellulose
Enzyme dosage is 1.3%, and hydrolysis temperature is 50 DEG C, and enzymatic hydrolysis pH value is 4.5, enzymolysis time 75min.
8. the method that enzymatic hydrolysis auxiliary reflux extracts Chinese lizardtail flavone as described in claim 1, which is characterized in that the step
(4) it dries for 24 hours.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101234156A (en) * | 2008-03-12 | 2008-08-06 | 北京星昊医药股份有限公司 | Saururus loureiri extract and preparation technique thereof |
| CN104840501A (en) * | 2015-05-21 | 2015-08-19 | 宁波好口味食品有限公司 | Preparation method for total flavonoids of chrysanthemum |
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2019
- 2019-06-14 CN CN201910515891.7A patent/CN110237143A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101234156A (en) * | 2008-03-12 | 2008-08-06 | 北京星昊医药股份有限公司 | Saururus loureiri extract and preparation technique thereof |
| CN104840501A (en) * | 2015-05-21 | 2015-08-19 | 宁波好口味食品有限公司 | Preparation method for total flavonoids of chrysanthemum |
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