CN110208041A - A kind of automatic sampling dilution process - Google Patents
A kind of automatic sampling dilution process Download PDFInfo
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- CN110208041A CN110208041A CN201910372584.8A CN201910372584A CN110208041A CN 110208041 A CN110208041 A CN 110208041A CN 201910372584 A CN201910372584 A CN 201910372584A CN 110208041 A CN110208041 A CN 110208041A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Hydrology & Water Resources (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to the technical field of dilution technique more particularly to a kind of automatic sampling dilution process, wherein includes the following steps: step S10, sample platform is moved to fluid infusion point, extract dilution blank solution from sample-adding pipeline with syringe and be expelled to sampling line;Step S11, syringe extracts amount of dilution from sample-adding pipeline;Step S12, sample platform is moved to back liquid point, is inserted back into liquid pipeline;Step S13, in test tube point, amount of dilution is discharged with sample needle;Step S14, sample platform is moved to back liquid point, blank solution will be diluted in syringe and is expelled in liquid back pipe road and abandons;Repetitive operation step S11-S14, until the liquid in all test tubes is diluted;Step S15, sample platform is moved to waste liquid point, syringe extracts air from sampling line and is discharged from sample-adding pipeline.The beneficial effect of technical solution of the present invention is: being automatically brought into operation reduction manpower waste by controlling equipment, reduces experimental error, improve the conventional efficient of experimenter.
Description
Technical field
The present invention relates to dilution technique technical field more particularly to a kind of automatic sampling dilution process.
Background technique
Procedure parameter is very important production target in biochemistry production, is related to the yield and quality etc. of final products,
Line sampling dilution technology can obtain a large amount of required parameters in process of production, ensure that technical parameter in production process
Stablize, controllably, it is also a large amount of artificial to be saved during production, while enriching with on-line parameter, it is also to produce intelligent control
There is provided may.
Conventional dissolution experiment is all only to take drug-eluting solution, and then operator is diluted manually, is carried out because artificial
Dilution wastes time to need to go to dilute manually later with larger dilution error, multichannel batch sampling, the human resources that can be generated
Waste and experimental error, the working efficiency of experimenter are lower.
Summary of the invention
For the above-mentioned problems in the prior art, one kind is now provided and is aimed to solve the problem that because being needed after multichannel batch sampling
It to go to dilute manually, and the automatic sampling dilution process the problem of waste of human resource and experimental error of generation
Specific technical solution is as follows:
The present invention provides a kind of automatic sampling dilution process, including:
The one solution dilution of automatic sampling dilution process, the solution dilution include the following steps:
Step S10, a sample platform is provided, the sample platform is moved to fluid infusion point, a syringe is provided and is added from one
The dilution blank solution of one first preset capacity is extracted in sample pipeline, and is expelled in a sampling line, is repeated at least three times, with clear
Wash remaining drug lysate in the sample-adding pipeline;
Step S11, the described syringe extracts the amount of dilution of setting from the sample-adding pipeline, on the basis of the amount of dilution
The upper liquid for extracting one second preset capacity more;
Step S12, the sample platform is moved to back liquid point, is inserted into a liquid back pipe road, a third is default to be held to be discharged
The liquid of amount;
Step S13, the sample platform is moved to test tube point, provides a sample needle to decline the finger for calculating the liquid
Fixed height, and the amount of dilution of setting is discharged, so that the syringe keeps the dilution of one the 4th preset capacity empty
White liquor;
Step S14, the sample platform is moved to described time liquid point, it will the default appearance of residual one the 5th in the syringe
The dilution blank solution of amount, is expelled in the liquid back pipe road and abandons;
Repetitive operation step S11-S14 is finished until the liquid in all test tubes of setting dilutes;
Step S15, after dilution, the sample platform is moved to waste liquid point, the syringe is from the sampling
Pipeline extracts the air of one the 6th preset capacity, is then discharged from the sample-adding pipeline, and at least repetitive operation is primary, with thoroughly clear
Remaining solution in the empty pipeline.
Preferably, the first preset capacity is set as 10ml;And/or
Second preset capacity is set as 1ml;And/or
Third preset capacity is set as 0.5ml;And/or
4th preset capacity is set as 0.5ml;And/or
5th preset capacity is set as 0.5ml;And/or
6th preset capacity is set as 10ml.
It preferably, further include that a solution sampling process and a solution mend sample process before Yu Suoshu solution dilution.
Preferably, the solution sampling process includes the following steps:
Step S20, a stripping rotor is provided, one the 7th preset capacity is extracted by the sampling line from the stripping rotor
Liquid, with to the syringe, the sampling line, the sample-adding pipeline and liquid back pipe road carry out rinse;
Step S21, the liquid dissolved that default specified amount is extracted in Yu Suoshu stripping rotor, in the default specified amount
On the basis of the liquid for extracting one the 8th preset capacity more;
Step S22, the liquid that one the 9th preset capacity is extracted in the sample-adding pipeline, will fill in the sample-adding pipeline
The drug completely dissolved, and solution when rinse is exhausted into the stripping rotor;
Step S23, the sample platform is moved to the test tube point, by calculating sampling amount, by the sample needle
A preset height is dropped to, and extracts specified sampling amount into test tube;
Step S24, the sample needle is connected to the liquid back pipe road, by the liquid assimilating of remaining 1 the tenth preset capacity
To in the stripping rotor, then the syringe switches to sampling valve, and the sampling probe is hanging, to extract the air, so
Switch to the sample-adding pipeline afterwards the liquid in the sampling line and the sample-adding pipeline to be discharged in the stripping rotor.
Preferably, the 7th preset capacity is set as 10ml;And/or
8th preset capacity is set as 3ml;And/or
9th preset capacity is set as 2.5ml;And/or
Tenth preset capacity is set as 0.5ml.
Preferably, the solution is mended sample process and is included the following steps:
Step S30, the sample platform is moved to the fluid infusion point, the sample needle declines and extracts the fluid infusion of setting
Then amount will mend sample platform and be moved to described time liquid point, by the Liquid extracting into the stripping rotor;
Step S31, the sampling probe is hanging and extract the air of 1 the 11st preset capacity, be then switched to it is described plus
Sample pipeline draws back the air to the sample-adding pipeline;
Repeating said steps S31 all draws back residual liquid in the pipeline into the stripping rotor.
Preferably, the 11st preset capacity is set as 5ml.
Preferably, it after the completion of the solution sampling process and solution benefit sample process all carry out, then carries out described molten
Liquid dilution.
Preferably, in step S21, the capacity for extracting the liquid of default specified amount dissolved is set as 0-
6.9ml。
Preferably, in step S11, the amount of dilution for extracting setting is set as 0-9ml.
The beneficial effect of technical solution of the present invention is: a kind of automatic sampling dilution process is provided, by controlling equipment
It is automatically brought into operation, drug solution is diluted to specified multiple, reduce manpower waste, reduce experimental error, to improve experiment people
The conventional efficient of member.
Detailed description of the invention
With reference to appended attached drawing, more fully to describe the embodiment of the present invention.However, appended attached drawing be merely to illustrate and
It illustrates, and is not meant to limit the scope of the invention.
Fig. 1 is the diluted apparatus structure schematic diagram of automatic sampling of the embodiment of the present invention;
Fig. 2 is a kind of solution dilution block diagram of automatic sampling dilution process of the embodiment of the present invention;
Fig. 3 is a kind of sampling process block diagram of automatic sampling dilution process of the embodiment of the present invention;
Fig. 4 is the benefit sample process steps figure of the on-line automatic sampling dilution technique method of one kind of the embodiment of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art without creative labor it is obtained it is all its
His embodiment, shall fall within the protection scope of the present invention.
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
The present invention will be further explained below with reference to the attached drawings and specific examples, but not as the limitation of the invention.
The present invention provides a kind of automatic sampling dilution process, including:
One solution dilution of automatic sampling dilution process, solution dilution include the following steps:
Step S10, a sample platform (not shown) is provided, sample platform (not shown) is moved to fluid infusion point
1, a syringe 2 is provided and extracts the dilution blank solution of one first preset capacity from a sample-adding pipeline 3, and is expelled to a probe tube
In road 4, repeat at least three times, with remaining drug lysate in cleaning sample-adding pipeline 3;
Step S11, syringe 2 extracts the amount of dilution of setting from sample-adding pipeline 3, on the basis of amount of dilution to extract one more
The liquid of second preset capacity;
Step S12, sample platform (not shown) is moved to back liquid point 5, a liquid back pipe road 50 is inserted into, to be discharged one
The liquid of third preset capacity;
Step S13, sample platform (not shown) is moved to test tube point 6, provides a sample needle 30 to decline and calculate
The specified altitude assignment of liquid, and the amount of dilution of setting is discharged, so that syringe 2 keeps the dilution blank of one the 4th preset capacity
Liquid;
Step S14, sample platform (not shown) is moved to back liquid point 5, residual one the 5th in syringe 2 is preset
The dilution blank solution of capacity, is expelled in liquid back pipe road 50 and abandons;
Repetitive operation step S11-S14 is finished until the liquid in all test tubes of setting dilutes;
Step S15, after dilution, sample platform (not shown) is moved to waste liquid point 7, syringe 2 is from taking
Sample pipeline 4 extracts the air of one the 6th preset capacity, is then discharged from sample-adding pipeline 3, and at least repetitive operation is primary, with thoroughly clear
Remaining solution in blank pipe road.
By a kind of automatic sampling dilution process of above-mentioned offer, in conjunction with shown in Fig. 1,2, first by syringe 2 in adding
The dilution blank solution of the first preset capacity is extracted in sample pipeline 3 and is expelled to sampling line 4, repeats at least three times, to add with cleaning
Remaining drug lysate in sample pipeline 3.
Further, syringe 2 extracts the amount of dilution of setting from sample-adding pipeline 3, and more pumpings on the basis of amount of dilution
Take the liquid of one second preset capacity.
Further, sample platform (not shown) is moved to back liquid point 5, is inserted into a liquid back pipe road 50, with discharge
The liquid of one third preset capacity, while avoiding sample needle 30 from having hanging drop, and the negative pressure of each pipeline can be mitigated, it reduces
The error of experiment, to improve the precision of experiment.
Further, sample platform (not shown) is moved to test tube point, provides a sample needle 30 to decline and calculate
The specified altitude assignment of liquid, and the amount of dilution of setting is discharged, so that syringe 2 keeps the dilution blank of one the 4th preset capacity
Liquid, sample platform (not shown) are moved to back liquid point 5 again, and the dilution blank solution of remaining 5th preset capacity is expelled to
It is abandoned in liquid back pipe road 50.
Further, aforesaid operations are repeated, until the drug solution of all test tubes of setting is all diluted to the multiple formulated.
Finally, sample platform (not shown) is moved to waste liquid point 7, syringe 2 extracts the 6th from sampling line 4
The air of preset capacity, then be discharged from sample-adding pipeline 3, it is aforesaid operations at least one time, molten with the residual in thorough emptying pipeline
Liquid.
In a kind of preferably case study on implementation, the first preset capacity is set as 10ml;And/or
Second preset capacity is set as 1ml;And/or
Third preset capacity is set as 0.5ml;And/or
4th preset capacity is set as 0.5ml;And/or
5th preset capacity is set as 0.5ml;And/or
6th preset capacity is set as 10ml.
Specifically, by accurate preset capacity, so that the error that automatic dilution process generates reduces, to reach high-precision
The dilution of degree.
It further include a solution sampling process and a solution before solution dilution in a kind of preferably case study on implementation
Mend sample process.
In a kind of preferably case study on implementation, solution sampling process includes the following steps:
Step S20, a stripping rotor 8 is provided, extracts the liquid of one the 7th preset capacity by sampling line 4 from stripping rotor 8
Body, to carry out rinse to syringe 2, sampling line 4, sample-adding pipeline 3 and liquid back pipe road 50;
Step S21, the liquid dissolved that default specified amount is extracted in stripping rotor 8, on the basis of default specified amount
The upper liquid for extracting one the 8th preset capacity more;
Step S22, the liquid that one the 9th preset capacity is extracted in sample-adding pipeline 3, will be loaded in pipeline 3 full of dissolution
Drug, and exhaust solution when rinse into stripping rotor 8;
Step S23, sample platform is moved to test tube point 6, by calculating sampling amount, it is pre- that sample needle 30 is dropped to one
If height, and specified sampling amount is extracted into test tube;
Step S24, sample needle 30 is connected to liquid back pipe road 50, by the liquid assimilating of remaining 1 the tenth preset capacity to molten
Out in cup 8, then syringe 2 switches to sampling valve 9, and sampling probe 40 is hanging, to extract air, is then switched to sample-adding pipeline
3 the liquid in sampling line 4 and sample-adding pipeline 3 to be discharged in stripping rotor 8.
Specifically, in conjunction with shown in Fig. 1,3, rinse first is carried out to syringe 2 and each pipeline;It is extracted from stripping rotor 8
The 0-6.9ml liquid dissolved, and extract 3ml more;The liquid of 2.5ml is then exhausted from into sample-adding pipeline 3, so that sample-adding pipeline
Solution in 3 full of the drug dissolved and when rinse is discharged is into stripping rotor 8.
Further, by calculating sampling amount, sample needle 30 is dropped into a preset height, preset height is set as rigid
The good liquid level for not crossing liquid influences experimental precision to prevent sample needle 30 from generating hanging drop, and extracts specified sampling amount to test tube
In.
Further, by the liquid assimilating of remaining 0.5ml into stripping rotor 8, then syringe 2 switches to sampling valve 9, takes
Sample needle is hanging, draw 5ml air, then switch to sample-adding pipeline 3, by sampling line 4 be loaded pipeline 3 in liquid be discharged to it is molten
Out in cup 8, it is ensured that liquor capacity variation is sampling amount in stripping rotor.
In a kind of preferably case study on implementation, the 7th preset capacity is set as 10ml;And/or
8th preset capacity is set as 3ml;And/or
9th preset capacity is set as 2.5ml;And/or
Tenth preset capacity is set as 0.5ml.
Specifically, by accurate preset capacity, so that the error that automatic dilution process generates reduces, to reach high-precision
The dilution of degree.
In a kind of preferably case study on implementation, solution is mended sample process and is included the following steps:
Step S30, sample platform (not shown) is moved to fluid infusion point 1, sample needle 30 declines and extracts setting
Then amount infused will mend sample platform (not shown) and be moved to back liquid point 5, by Liquid extracting into the stripping rotor 8;
Step S31, sampling probe 40 is hanging and extract the air of 1 the 11st preset capacity, it is then switched to sample-adding pipeline
3, air is drawn back to sample-adding pipeline 3;
Step S31 is repeated, residual liquid in pipeline is all drawn back into stripping rotor 8.
Specifically, in conjunction with shown in Fig. 1,4, sample platform (not shown) is moved to fluid infusion point 1, and sample needle 30 declines simultaneously
The amount infused of setting is extracted, sample platform (not shown) is then mended and is moved to back liquid point 5, by Liquid extracting into stripping rotor 8.
Further, then it being switched to sampling valve 9, sampling probe is hanging, draws 5ml air, sample-adding pipeline 3 is then switched to,
Air is drawn back to sample-adding pipeline 3, repeat it is above draw 10ml air, then sample-adding pipeline 3 is drawn back, so as to will be remaining in pipeline
Liquid is all extracted into stripping rotor 8, to guarantee the accurate of amount infused.
In a kind of preferably case study on implementation, the 11st preset capacity is set as 5ml.
In a kind of preferably case study on implementation, after the completion of solution sampling process is all carried out with solution benefit sample process, then into
Row solution dilution.
Specifically, it in conjunction with shown in 1-4 figure, was diluted again after the completion of sampling process and benefit sample process all carry out
Journey dilutes so that dilution reduces experimental error to reach high-precision.
In a kind of preferably case study on implementation, in step S21, the appearance for the liquid of default specified amount dissolved is extracted
Amount is set as 0-6.9ml.
In a kind of preferably case study on implementation, in step S11, the amount of dilution for extracting setting is set as 0-9ml.
The foregoing is merely preferred embodiments of the present invention, are not intended to limit embodiments of the present invention and protection model
It encloses, to those skilled in the art, should can appreciate that all with made by description of the invention and diagramatic content
Equivalent replacement and obviously change obtained scheme, should all be included within the scope of the present invention.
Claims (10)
1. a kind of automatic sampling dilution process, which is characterized in that the one solution dilution of automatic sampling dilution process, it is described
Solution dilution includes the following steps:
Step S10, a sample platform is provided, the sample platform is moved to fluid infusion point, provides a syringe from a sample-adding pipe
The dilution blank solution of one first preset capacity is extracted in road, and is expelled in a sampling line, is repeated at least three times, to clean
State remaining drug lysate in sample-adding pipeline;
Step S11, the described syringe extracts the amount of dilution of setting from the sample-adding pipeline, more on the basis of the amount of dilution
Extract the liquid of one second preset capacity;
Step S12, the sample platform is moved to back liquid point, is inserted into a liquid back pipe road, a third preset capacity is discharged
Liquid;
Step S13, the sample platform is moved to test tube point, provides a sample needle to decline the specified height for calculating the liquid
Degree, and the amount of dilution of setting is discharged, so that the syringe keeps the dilution blank solution of one the 4th preset capacity;
Step S14, the sample platform is moved to described time liquid point, one the 5th preset capacity will be remained in the syringe
The dilution blank solution is expelled in the liquid back pipe road and abandons;
Repetitive operation step S11-S14 is finished until the liquid in all test tubes of setting dilutes;
Step S15, after dilution, the sample platform is moved to waste liquid point, the syringe is from the sampling line
The air of one the 6th preset capacity is extracted, is then discharged from the sample-adding pipeline, at least repetitive operation is primary, thoroughly to empty institute
State remaining solution in pipeline.
2. a kind of automatic sampling dilution process according to claim 1, which is characterized in that the first preset capacity is set as
10ml;And/or
Second preset capacity is set as 1ml;And/or
Third preset capacity is set as 0.5ml;And/or
4th preset capacity is set as 0.5ml;And/or
5th preset capacity is set as 0.5ml;And/or
6th preset capacity is set as 10ml.
3. a kind of automatic sampling dilution process according to claim 1, which is characterized in that Yu Suoshu solution dilution it
Before further include that a solution sampling process and a solution mend sample process.
4. a kind of automatic sampling dilution process according to claim 3, which is characterized in that the solution sampling process includes
Following steps:
Step S20, a stripping rotor is provided, extracts the liquid of one the 7th preset capacity by the sampling line from the stripping rotor
Body, to carry out rinse to the syringe, the sampling line, the sample-adding pipeline and liquid back pipe road;
Step S21, the liquid dissolved that default specified amount is extracted in Yu Suoshu stripping rotor, in the base of the default specified amount
The liquid for extracting one the 8th preset capacity on plinth more;
Step S22, the liquid of one the 9th preset capacity is extracted in the sample-adding pipeline, it is molten by being full of in the sample-adding pipeline
The drug of solution, and solution when rinse is exhausted into the stripping rotor;
Step S23, the sample platform is moved to the test tube point, by calculating sampling amount, the sample needle is declined
To a preset height, and specified sampling amount is extracted into test tube;
Step S24, the sample needle is connected to the liquid back pipe road, by the liquid assimilating of remaining 1 the tenth preset capacity to institute
It states in stripping rotor, then the syringe switches to sampling valve, and sampling probe is hanging, to extract the air, it is then switched to
The sample-adding pipeline is the liquid in the sampling line and the sample-adding pipeline to be discharged in the stripping rotor.
5. a kind of automatic sampling dilution process according to claim 4, which is characterized in that the 7th preset capacity is set as
10ml;And/or
8th preset capacity is set as 3ml;And/or
9th preset capacity is set as 2.5ml;And/or
Tenth preset capacity is set as 0.5ml.
6. a kind of automatic sampling dilution process according to claim 3, which is characterized in that the solution mends sample process and includes
Following steps:
Step S30, the sample platform being moved to the fluid infusion point, the sample needle declines and extracts the amount infused of setting,
Then sample platform will be mended and be moved to described time liquid point, by the Liquid extracting into the stripping rotor;
Step S31, the sampling probe is hanging and extract the air of 1 the 11st preset capacity, it is then switched to the sample-adding and manages
Road draws back the air to the sample-adding pipeline;
Repeating said steps S31 all draws back residual liquid in the pipeline into the stripping rotor.
7. a kind of automatic sampling dilution process according to claim 6, which is characterized in that the 11st preset capacity is set
It is set to 5ml.
8. a kind of automatic sampling dilution process according to claim 1, which is characterized in that the solution sampling process and institute
It states solution to mend after the completion of sample process all carries out, then carries out the solution dilution.
9. a kind of automatic sampling dilution process according to claim 4, which is characterized in that in step S21, extract default
The capacity for the liquid of specified amount dissolved is set as 0-6.9ml.
10. a kind of automatic sampling dilution process according to claim 1, which is characterized in that in step S11, extraction is set
The fixed amount of dilution is set as 0-9ml.
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Cited By (1)
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Application publication date: 20190906 |