CN110156173A - A kind of method that Immobilized cells culture removes nitrogen phosphorus in sewage - Google Patents

A kind of method that Immobilized cells culture removes nitrogen phosphorus in sewage Download PDF

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Publication number
CN110156173A
CN110156173A CN201910455288.4A CN201910455288A CN110156173A CN 110156173 A CN110156173 A CN 110156173A CN 201910455288 A CN201910455288 A CN 201910455288A CN 110156173 A CN110156173 A CN 110156173A
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immobilized cells
nitrogen phosphorus
cells culture
algae
pva
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宋东辉
刘志敏
刘娜
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Tianjin University of Science and Technology
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Tianjin University of Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Molecular Biology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to the methods of nitrogen phosphorus in a kind of Immobilized cells culture removal sewage, by the algae solution centrifugation after culture, supernatant is removed together, it is rinsed twice with deionized water, removes the inorganic salts of cell residue, the active carbon of 1-4wt% is added, adsorb 10min, it is then added in the mixed liquor of sodium alginate, PVA, forms the algae gel dissolved with 1-5wt% sodium alginate and 1-5wt%PVA, be added dropwise to 1-5wt%CaCl2With 1-4wt% boric acid, immobilization is crosslinked 1-4h, is rinsed 2-3 times with deionized water, finally obtains the immobilized spherule that diameter is about 4mm；The algae cell density of Immobilized cells culture is controlled 2.0~4.0 × 106Between a/ball, starvation time is 24~72h, is put into sewage.Select stationary phase algae, chlorella cells density 3.20 × 106A/ball, starvation time 51.84h, N/P ratio 26.72, glucose content 0.47wt%, with this condition, nitrogen removal efficiency is 92.86% in waste water, and Phosphorus From Wastewater removal rate is 97.32%, and higher nitrogen phosphorus ligands ability is shown compared with on-fixed chlorella.

Description

A kind of method that Immobilized cells culture removes nitrogen phosphorus in sewage
Technical field
The invention belongs to sewage treatment fields, are related to Immobilized cells culture technology, especially a kind of for removing in sewage The method of nitrogen phosphorus fixation chlorella.
Background technique
Algae can be using a variety of inorganic and organonitrogen compound as nitrogen source, with H2P04 OneAnd HPO4 2-The Phos of form is made It is phosphorus source for growing.So microalgae system is to effectively remove nitrogen, phosphorus compound and the sewage depth for causing eutrophication problem Degree processing provides an excellent solution.
Chlorella Chlorella belongs to unicellular alga, be Chlorophyta, Chlorococcale, one in chlorella section it is important Category.This several years, China, which also starts, to be concerned about to chlorella applied to manufacture.But because chlorella possess it is extremely firm thin Cell wall, not only containing pole multifilament element, while also with the homologue of sporopollenin, to the methods of organic solvent permeation cell wall Extremely disadvantageous, all utility ratios that it is obtained are also low in the extreme.
Summary of the invention
It is an object of the invention in place of overcome the deficiencies in the prior art, provide a kind of effect good, both economical, easy behaviour The method of nitrogen phosphorus in the Immobilized cells culture removal sewage of work, nitrogen removal efficiency is 92.86% in waste water, Phosphorus From Wastewater removal rate It is 97.3%.
The technical proposal for solving the technical problem of the invention is:
A kind of method that Immobilized cells culture removes nitrogen phosphorus in sewage, the algae solution after culture is centrifuged, removes supernatant together, is used Deionized water is rinsed twice, and the inorganic salts of cell residue are removed, and the active carbon of 1-4wt% is added, and is adsorbed 10min, is then added to Sodium alginate, PVA mixed liquor in, formed dissolved with 1-5wt% sodium alginate and 1-5wt%PVA algae gel, be added dropwise to 1- 5wt% CaCl2With 1-4wt% boric acid, immobilization is crosslinked 1-4h, is rinsed 2-3 times with deionized water, finally obtaining diameter is about The immobilized spherule of 4mm；The algae cell density of Immobilized cells culture is controlled 2.0~4.0 × 106Between a/ball, when hungry Between be 24~72h, put into sewage.
Moreover, the additional amount of active carbon is 1.5wt%.
Moreover, the mass percentage of sodium alginate is 2wt%.
Moreover, the mass percentage of PVA is 1.5wt%.
Moreover, CaCl2Mass percentage be 2wt%.
Moreover, the mass percentage of boric acid is 4wt%.
Moreover, immobilization crosslinking time is 3h.
Moreover, algae cell density is 3.20 × 106A/ball.
Moreover, starvation time 51.84h.
Moreover, the N/P ratio in sewage is 15~35:1, glucose content is 0.25wt%~0.75wt%.
The advantages and positive effects of the present invention are:
1, the polyvinyl alcohol for adding suitable concentration can guarantee the mechanical strength of immobilized spherule, the addition energy of sodium alginate Enough change the adhesion phenomenon of immobilized spherule, addition active carbon can change the mass-transfer performance of bead, and make immobilization small The forming of ball and intensity are influenced by a part.
2, stationary phase algae, chlorella cells density 3.20 × 10 are selected6A/ball, starvation time 51.84h, N/P ratio 26.72, glucose content 0.47wt%, with this condition, nitrogen removal efficiency is 92.86% in waste water, Phosphorus From Wastewater removal rate It is 97.32%, higher nitrogen phosphorus ligands ability is shown compared with on-fixed chlorella.
Specific embodiment
Below by specific embodiment, the invention will be further described, and it is not limit that following embodiment, which is descriptive, Qualitatively, this does not limit the scope of protection of the present invention.
Algae used in the present invention is the chlorella that University Of Science and Technology Of Tianjin, miniature organism product research department, ocean saves Chlorella sp. KDF-1 is isolated from TEDA in Tianjin development zone sewage treatment plant wastewater collection pond.
Culture medium is the SE culture medium of improvement: 0.75g/LNaNO3, 0.23g/LMgSO4·7H2O, 0.23g/L K2HPO4, 0.53g/L, KH2PO4, 0.03g/L NaCl, 0.01g/L FeSO4·6H2O, 2.0mL/L Fe-EDTA, 1.0mL/LA5 solution。
Using the Dauta culture medium of not nitrogenous and phosphorus as background, separately plus NH4Cl and NaH2PO4It prepares useless with Tianjin municipal administration life NH in water4 +- N and PO4 3-The artificial wastewater that-P concentration is consistent, pH value are 7.2 ± 0.1.
Embodiment 1
Algae solution after 300ml is cultivated is centrifuged 10min at 3500r/min, removes supernatant together, rinses two with deionized water It is secondary, the inorganic salts of cell residue are removed, the active carbon of 1.5wt% is added, 10min is adsorbed, is then added to the mixed liquor of SA, PVA In, the algae gel dissolved with 2wt%SA and 1.5wt%PVA is formed, 2wt%CaCl is added dropwise to2With 4wt% boric acid, immobilization is handed over Join 3h, rinsed 2-3 times with deionized water, finally obtains the immobilized spherule that diameter is 4mm or so.
Control chlorella cells density 3.20 × 106A/ball, starvation time 51.84h, N/P ratio 26.72 in waste water, Portugal Grape sugared content is 0.47wt%, and with this condition, nitrogen removal efficiency is 92.86% in waste water, and Phosphorus From Wastewater removal rate is 97.32%.
Embodiment 2
300ml culture algae solution is centrifuged 10min at 3500r/min, supernatant is removed, is rinsed twice, removed with deionized water The active carbon of 1.0wt% is added in the inorganic salts for removing cell residue, adsorbs 10min, is then added in the mixed liquor of SA, PVA, shape At the algae gel dissolved with 2.0wt%SA and 1.0wt%PVA, it is added dropwise to 2.0wt%CaCl2With 1.0wt% boric acid, immobilization is handed over Join 2.0h, rinsed 2-3 times with deionized water, finally obtains the immobilized spherule that diameter is 4mm or so.
Control chlorella cells density 2.0 × 106A/ball, starvation time 50.0h, N/P ratio 25.6 in waste water, glucose Content is 0.50wt%, and with this condition, nitrogen removal efficiency is 90.68% in waste water, and Phosphorus From Wastewater removal rate is 95.37%.
Embodiment 3
Algae solution after 300ml is cultivated is centrifuged 10min at 3500r/min, removes supernatant, rinses two with deionized water It is secondary, the inorganic salts of cell residue are removed, the active carbon of 2.0wt% is added, 10min is adsorbed, is then added to the mixed liquor of SA, PVA In, the algae gel dissolved with 3.0wt%SA and 3.0wt%PVA is formed, 1.5wt%CaCl is added dropwise to2With 2.0wt% boric acid, Gu Fixedization is crosslinked 3.0h, is rinsed 2-3 times with deionized water, finally obtains the immobilized spherule that diameter is 4mm or so.
Control chlorella cells density 2.2 × 106A/ball, starvation time 51.3h, N/P ratio 26.4 in waste water, glucose Content is 0.48wt%, and with this condition, nitrogen removal efficiency is 92.6% in waste water, and Phosphorus From Wastewater removal rate is 95.37%.
Embodiment 4
Algae solution after 300ml is cultivated is centrifuged 10min at 3500r/min, removes supernatant together, rinses two with deionized water It is secondary, the inorganic salts of cell residue are removed, the active carbon of 1.5wt% is added, 10min is adsorbed, is then added to the mixed liquor of SA, PVA In, the algae gel dissolved with 2.5wt%SA and 2.0wt%PVA is formed, 3.0wt%CaCl is added dropwise to2With 1.5wt% boric acid, Gu Fixedization is crosslinked 3h, is rinsed 2-3 times with deionized water, finally obtains the immobilized spherule that diameter is 4mm or so.
Control chlorella cells density 1.9 × 106A/ball, starvation time 52.1h, N/P ratio 24.9 in waste water, glucose Content is 0.51wt%, and with this condition, nitrogen removal efficiency is 91.4% in waste water, and Phosphorus From Wastewater removal rate is 95.3%.
Embodiment 5
Algae solution after 300ml is cultivated is centrifuged 10min at 3500r/min, removes supernatant together, rinses two with deionized water It is secondary, the inorganic salts of cell residue are removed, the active carbon of 1.5wt% is added, 10min is adsorbed, is then added to the mixed liquor of SA, PVA In, the algae gel dissolved with 3.0wt%SA and 2.0wt%PVA is formed, 2.0wt%CaCl is added dropwise to2With 2.5wt% boric acid, Gu Fixedization is crosslinked 4.5h, is rinsed 2-3 times with deionized water, finally obtains the immobilized spherule that diameter is 4mm or so.
Control chlorella cells density 2.6 × 106A/ball, starvation time 51.6h, N/P ratio 25.4 in waste water, glucose Content is 0.56wt%, and with this condition, nitrogen removal efficiency is 92.9% in waste water, and Phosphorus From Wastewater removal rate is 96.5%.
Embodiment 6
Algae solution after 300ml is cultivated is centrifuged 10min at 3500r/min, removes supernatant together, rinses two with deionized water It is secondary, the inorganic salts of cell residue are removed, the active carbon of 3.0wt% is added, 10min is adsorbed, is then added to the mixed liquor of SA, PVA In, the algae gel dissolved with 3.5wt%SA and 3.0wt%PVA is formed, 1.5wt%CaCl is added dropwise to2With 2.5wt% boric acid, Gu Fixedization is crosslinked 3.5h, is rinsed 2-3 times with deionized water, finally obtains the immobilized spherule that diameter is 4mm or so.
Control chlorella cells density 2.8 × 106A/ball, starvation time 52.6h, N/P ratio 26.1 in waste water, glucose Content is 0.42wt%, and with this condition, nitrogen removal efficiency is 88.8% in waste water, and Phosphorus From Wastewater removal rate is 94.8%.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art For, under the premise of not departing from inventive concept, various modifications and improvements can be made, these belong to guarantor of the invention Protect range.

Claims (10)

1. a kind of method of nitrogen phosphorus in Immobilized cells culture removal sewage, it is characterised in that: be centrifuged the algae solution after culture, go together Supernatant is rinsed twice with deionized water, removes the inorganic salts of cell residue, and the active carbon of 1-4wt% is added, and adsorbs 10min, It is then added in the mixed liquor of sodium alginate, PVA, forms the algae gel dissolved with 1-5wt% sodium alginate and 1-5wt%PVA, drop Enter to 1-5wt%CaCl2With 1-4wt% boric acid, immobilization is crosslinked 1-4h, is rinsed 2-3 times with deionized water, finally obtains diameter The about immobilized spherule of 4mm；The algae cell density of Immobilized cells culture is controlled 2.0~4.0 × 106It is hungry between a/ball Time is 24~72h, is put into sewage.
2. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: active carbon Additional amount is 1.5wt%.
3. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: sodium alginate Mass percentage be 2wt%.
4. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: the matter of PVA Amount percentage composition is 1.5wt%.
5. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: CaCl2Matter Amount percentage composition is 2wt%.
6. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: the matter of boric acid Amount percentage composition is 4wt%.
7. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: immobilization is handed over The connection time is 3h.
8. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: frustule is close Degree is 3.20 × 106A/ball.
9. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: starvation time 51.84h。
10. the method for nitrogen phosphorus in Immobilized cells culture removal sewage according to claim 1, it is characterised in that: in sewage N/P ratio be 15~35:1, glucose content be 0.25wt%~0.75wt%.
CN201910455288.4A 2019-05-29 2019-05-29 A kind of method that Immobilized cells culture removes nitrogen phosphorus in sewage Withdrawn CN110156173A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2242030C (en) * 1997-07-01 2002-01-15 Samsung General Chemicals Co., Ltd. Alginate gel based adsorbents for heavy metal removal
DE19918953C2 (en) * 1999-04-27 2003-08-28 Rainer Buchholz Particulate construct with biomass and its use
CN101475931A (en) * 2009-01-22 2009-07-08 厦门大学 Preparation of embedding immobilized effective microorganism gel bead
CN109395699A (en) * 2018-11-14 2019-03-01 浙江海洋大学 A method of waste water is handled using microalgae and recycles phosphorus element
CN109534513A (en) * 2018-12-28 2019-03-29 厦门理工学院 The bacterium algae immobilization preparation purified in situ aquiculture waste water method that charcoal is strengthened

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2242030C (en) * 1997-07-01 2002-01-15 Samsung General Chemicals Co., Ltd. Alginate gel based adsorbents for heavy metal removal
DE19918953C2 (en) * 1999-04-27 2003-08-28 Rainer Buchholz Particulate construct with biomass and its use
CN101475931A (en) * 2009-01-22 2009-07-08 厦门大学 Preparation of embedding immobilized effective microorganism gel bead
CN109395699A (en) * 2018-11-14 2019-03-01 浙江海洋大学 A method of waste water is handled using microalgae and recycles phosphorus element
CN109534513A (en) * 2018-12-28 2019-03-29 厦门理工学院 The bacterium algae immobilization preparation purified in situ aquiculture waste water method that charcoal is strengthened

Non-Patent Citations (2)

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Title
胡晓旭: "不同小球藻的固定化及对污水中氮磷营养盐的利用研究", 《中国优秀硕士论文全文数据库 工程科技I辑》 *
陈娟: "固定化小球藻及其对氮磷利用的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 *

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Application publication date: 20190823