CN110150189A - A kind of artificial fecundation method of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body - Google Patents
A kind of artificial fecundation method of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body Download PDFInfo
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- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 18
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 18
- 210000004681 ovum Anatomy 0.000 claims abstract description 18
- 230000036541 health Effects 0.000 claims abstract description 6
- 230000009027 insemination Effects 0.000 claims abstract description 6
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- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 241000237858 Gastropoda Species 0.000 claims 1
- 235000013372 meat Nutrition 0.000 claims 1
- 230000004720 fertilization Effects 0.000 abstract description 13
- 241000282376 Panthera tigris Species 0.000 abstract description 5
- 230000013011 mating Effects 0.000 abstract description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 abstract description 3
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- 230000000052 comparative effect Effects 0.000 description 16
- 239000007788 liquid Substances 0.000 description 7
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- 241000357439 Epinephelus Species 0.000 description 2
- 241001560086 Pachyrhizus Species 0.000 description 2
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- 241001217936 Epinephelus lanceolatus Species 0.000 description 1
- 241000015901 Epinephelus tukula Species 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
The present invention provides the artificial fecundation method of a kind of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body, selects 5 healthy epinephelus fuscoguttatus more than age as female parent；Select the blue big spot grouper of body of 7 health more than age as male parent；And cultivation reinforcing is carried out respectively, prelarva is obtained by artificial insemination, hatching.Using mating system of the present invention, optimizes the ovum quality of epinephelus fuscoguttatus and the sperm quality of the big spot grouper of blue body, significantly improve rate of fertilization and hatching rate, establish good basis to obtain elite hybrid gold tiger spot.
Description
Technical field
The present invention relates to grouper reproduction technique field, in particular to a kind of epinephelus fuscoguttatus and the big spot grouper of blue body are miscellaneous
Hand over the artificial fecundation method of kind.
Background technique
Epinephelus fuscoguttatus (scientific name: Epinephelus fuscoguttatus), is subordinate to Perciformes, Sushi section, Epinephelus,
Also known as tiger spot, brown grouper are distributed in Red sea, the eastern bank in Indian Ocean Africa to Pacific Ocean Polynesia, north reaches Ryukyu group
Island, south to the sea areas such as Australian, the island of Taiwan and the South China Sea Islands, the Penghu Archipelago etc., inhabit 1-60 meters of depth, body length is reachable
120 centimetres, belong to water warm offshore and coral fishes, lives in the limpid coral reef of water quality, lagoon.It is carnivorous, with fish,
Siphonopods, shell-fish are food, can be used as food fish, cultivation fish and ornamental fish.Epinephelus fuscoguttatus is ingested, and violent, growth is fast, extensively
Applied to breeding production.
The blue big spot grouper of body, Epinephelus tukula, popular name money spot, blackspot grouper are spoke fin net-rope perch
The fish of shape mesh perch Ya Mu Sushi section Epinephelus, figure are only second to epinephelus lanceolatus fish, and growth is very fast, are the stone of great cultivation future
Spot fish species.It is distributed in India's Atlantic ocean region-west, from Red sea, East Africa to Australia Queensland sea area, inhabites 10-150 meters of depth, body
Long 200 centimetres reachable, weight is the rare warm water fishes in the world up to 110 kilograms.
New varieties gold tiger spot can be obtained by female epinephelus fuscoguttatus and the big spot grouper hybridization of male blue body.At present for golden tiger spot
The correlative study of artificial hybridization breeding technique is less, and it is relatively low to obtain fertility rate and hatchability using conventional hybridizing method.
Therefore it is badly in need of the artificial fecundation method of a kind of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body, solve above-mentioned technical problem.
Summary of the invention
For mirror with this, the present invention proposes the artificial fecundation method of a kind of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body.
The technical scheme of the present invention is realized as follows:
A kind of artificial fecundation method of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body, comprising the following steps:
(1) Juvenile stage:
A, female parent: select 5 healthy epinephelus fuscoguttatus more than age as female parent；
B, male parent: select the blue big spot grouper of body of 7 health more than age as male parent；
(2) epinephelus fuscoguttatus parent culture is strengthened:
A, nurturing an environment: selecting outdoor pond, and 3~4m of the depth of water cultivates 200~300 tails of density/mu, female-male proportion 1:
(0.5~0.8)；
B, feed A and feed B are fed,
The feed A by weight, including following component: 2~6 parts of mackerel, 8~15 parts of saury, spiral shell 3~8
Part and 1~3 part of Carnis Limax, feed daily 3~5 times, each feeding volume be fish body weight 0.8~1.5%；
The feed B is man-made feeds, by weight, including following component: 5~10 parts of chicken droppings cane leaves powder, salt algae powder
0.7~1.2 part, split 3~6 parts of pot algae powder and 0.5~0.8 part of vitamin E, feed 1 time within every 2~3 days, the feed B feeding volume
It is the 0.9~1.2% of fish body weight；
(3) cultivation of Lan Shen great spot grouper parent is strengthened
A, nurturing an environment: selecting outdoor pond, and 5~10m of the depth of water cultivates 60~75 tails of density/mu, female-male proportion 1:(2
~3)；
B, feed C and feed D are fed,
The feed C by weight, including following component: 8~14 parts of black cloth fish, 5~8 parts of Spanish mackerel, oyster 3~5
Part and 0.3~0.5 part of allicin, feed daily 1~2 time, each feeding volume be parent population weight 1~2%；
The feed D is man-made feeds, by weight, including following component: 80~90 parts of end of the bridge tapioca flour, coconut powder
4~7 parts, 1~2 part of pine needle meal, 2~8 parts of sargassum powder, 0.2~0.6 part of vitamin A, 0.2~0.3 part of vitamin C and dimension life
0.3~0.5 part of plain E is fed 1 time for every 2~3 days, and each feeding volume is the 2~3% of parent population weight；
(4) the sexually matured judgement of parent population
A, maturity inspection is carried out to female parent population weekly；Appearance upper abdomen protuberance, gonopore is micro- red, i.e., attainability at
It is ripe；
B, maturity inspection is carried out to male parent population weekly；Gonopore is micro- red, gently presses abdomen, can squeeze out milky essence
Liquid reaches sexal maturity；
(5) artificial insemination
Female epinephelus fuscoguttatus ovum and the big spot grouper sperm of the blue body of male are acquired respectively；Into the container for filling ovum
Clean seawater is added, on one side plus seawater while be gently mixed, add the big spot grouper sperm of blue body, mix, and in 30~
Vibratory stimulation 10~30 seconds under the conditions of 50Hz stand 1~2min, with seawater flushing fertilized eggs；
(6) hatching of fertilized eggs
It will be put into hatching barrel and hatch by step (5) processed fertilized eggs, incubating oosperm condition is dissolved oxygen in water
For 5~8mg/L, 25~28 DEG C of water temperature, salinity 28~31, pH7.0~8.0 obtain prelarva after hatching.
Further, in step (2), the feed A by weight, including following component: 5 parts of mackerel, saury 11
Part, 5 parts of spiral shell and 2 parts of Carnis Limax.
Further, in step (2), the feed A is fed 5 times daily, and each feeding volume is the 1.3% of fish body weight.
Further, in step (2), the feed B is cubed feed, by weight, including following component: Paederia scandens
7 parts of leaf powder, splits 4 parts of pot algae powder and 0.6 part of vitamin E at 0.9 part of salt algae powder.
Further, in step (3), the feed C by weight, including following component: 11 parts of black cloth fish, Spanish mackerel
7 parts, 4 parts of oyster and 0.4 part of allicin.
Further, in step (3), the feed D by weight, including following component: 87 parts of end of the bridge tapioca flour, coconut palm
5 parts of sub- powder, 1.2 parts of pine needle meal, 5 parts of sargassum powder, 0.4 part of vitamin A, 0.25 part of vitamin C and 0.4 part of vitamin E.
Further, in step (5), sperm additional amount is per kilogram ovum 1.1~1.3mL sperm；Seawater salinity is 28
~31, seawater amount of water is 0.9~1.1 times of semen volume.
Further, in step (5), the vibration frequency is 47Hz, and the vibratory stimulation time is 20 seconds.
Further, in step (6), hatching density is 280~3,000,000 fertilized eggs/m3。
Further, in step (6), change within every 2~3 hours that water is primary, each quantity of exchanged water be hatching barrel volume 30~
50%.
Compared with prior art, the beneficial effects of the present invention are:
(1) mating system of the present invention is used, the ovum quality of epinephelus fuscoguttatus and the essence of the big spot grouper of blue body are optimized
Protonatomic mass significantly improves rate of fertilization and hatching rate, establishes good basis to obtain elite hybrid gold tiger spot.
(2) mating system of the present invention is used, the egg laying amount of parent population is high, and for rate of fertilization up to 98%, the hatching rate of fertilized eggs can
Up to 95%.
(3) present invention feeds feed A combination feed B for epinephelus fuscoguttatus, and feeds for the big spot grouper of blue body
Feed C combination feed D optimizes the ovum quality of epinephelus fuscoguttatus and the sperm quality of the big spot grouper of blue body, significantly improves
Rate of fertilization and hatching rate.
(4) present invention uses vibratory stimulation, significantly improves rate of fertilization, and improve Fertilized quality, improves hatching rate.
Specific embodiment
In order to be best understood from the technology of the present invention content, specific embodiment is provided below, the present invention is described further.
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.
Material used in the embodiment of the present invention, reagent etc., are commercially available unless otherwise specified.
End of the bridge pachyrhizus of the invention is purchased from Hainan Province Dengmai County end of the bridge pachyrhizus.
Embodiment 1
A kind of artificial fecundation method of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body, comprising the following steps:
(1) Juvenile stage:
A, female parent: select 5 healthy epinephelus fuscoguttatus more than age as female parent；
B, male parent: select the blue big spot grouper of body of 7 health more than age as male parent；
(2) epinephelus fuscoguttatus parent culture is strengthened:
A, nurturing an environment: selecting outdoor pond, and 3~4m of the depth of water cultivates density about 200 tails/mu, female-male proportion 1:0.5；
B, feed A and feed B are fed,
The feed A by weight, including following component: 2 parts of mackerel, 15 parts of saury, 3 parts of spiral shell and Carnis Limax
It 3 parts, feeds daily 3 times, each feeding volume is the 1.5% of fish body weight；
The feed B is man-made feeds, by weight, including following component: 5 parts of chicken droppings cane leaves powder, salt algae powder 0.7
Part splits 6 parts of pot algae powder and 0.5 part of vitamin E, feeds 1 time within every 2 days, and the feed B feeding volume is the 1.2% of fish body weight；
(3) cultivation of Lan Shen great spot grouper parent is strengthened
A, nurturing an environment: selecting outdoor pond, and 5~10m of the depth of water cultivates density about 60 tails/mu, female-male proportion 1:2；
B, feed C and feed D are fed,
The feed C by weight, including following component: 8 parts of black cloth fish, 8 parts of Spanish mackerel, 3 parts of oyster and allicin
It 0.5 part, feeds daily 1 time, each feeding volume is the 2% of parent population weight；
The feed D is man-made feeds, by weight, including following component: 80 parts of end of the bridge tapioca flour, 4 parts of coconut powder,
1 part of pine needle meal, 8 parts of sargassum powder, 0.2 part of vitamin A, 0.3 part of vitamin C and 0.3 part of vitamin E are fed 1 time for every 2 days,
Each feeding volume is the 3% of parent population weight；
(4) the sexually matured judgement of parent population
A, maturity inspection is carried out to female parent population weekly；Appearance upper abdomen protuberance, gonopore is micro- red, i.e., attainability at
It is ripe；
B, maturity inspection is carried out to male parent population weekly；Gonopore is micro- red, gently presses abdomen, can squeeze out milky essence
Liquid reaches sexal maturity；
(5) artificial insemination
Female epinephelus fuscoguttatus ovum and the big spot grouper sperm of the blue body of male are acquired respectively；Into the container for filling ovum
Clean seawater is added, amount of water is identical as semen volume, on one side plus seawater while be gently mixed, add the big spot lithosporic milt of blue body
Liquid, sperm additional amount are per kilogram ovum 1.1mL sperm, mix, and vibratory stimulation 30 seconds under the conditions of 30Hz, stand 1min,
With seawater flushing fertilized eggs, the seawater salinity is 28~31；
(6) hatching of fertilized eggs
It will be put into hatching barrel and hatch by step (5) processed fertilized eggs, incubating oosperm condition is dissolved oxygen in water
For 5~8mg/L, 25~28 DEG C of water temperature, salinity 28~31, pH7.0~8.0, hatching density is about 2,800,000 fertilized eggs/m3, often
Change within 2 hours that water is primary, each quantity of exchanged water is the 30% of hatching barrel volume, obtains prelarva after hatching.
Embodiment 2
A kind of artificial fecundation method of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body, comprising the following steps:
(1) Juvenile stage:
A, female parent: select 5 healthy epinephelus fuscoguttatus more than age as female parent；
B, male parent: select the blue big spot grouper of body of 7 health more than age as male parent；
(2) epinephelus fuscoguttatus parent culture is strengthened:
A, nurturing an environment: selecting outdoor pond, and 3~4m of the depth of water cultivates density about 300 tails/mu, female-male proportion 1:0.8；
B, feed A and feed B are fed,
The feed A by weight, including following component: 6 parts of mackerel, 8 parts of saury, 8 parts of spiral shell and Carnis Limax 1
Part, it feeds daily 5 times, each feeding volume is the 0.8% of fish body weight；
The feed B is man-made feeds, by weight, including following component: 10 parts of chicken droppings cane leaves powder, salt algae powder 1.2
Part splits 3 parts of pot algae powder and 0.8 part of vitamin E, feeds 1 time within every 3 days, and the feed B feeding volume is the 0.9% of fish body weight；
(3) cultivation of Lan Shen great spot grouper parent is strengthened
A, nurturing an environment: selecting outdoor pond, and 5~10m of the depth of water cultivates 75 tails of density/mu, female-male proportion 1:3；
B, feed C and feed D are fed,
The feed C by weight, including following component: 14 parts of black cloth fish, 5 parts of Spanish mackerel, 5 parts of oyster and allicin
It 0.3 part, feeds daily 2 times, each feeding volume is the 1% of parent population weight；
The feed D is man-made feeds, by weight, including following component: 90 parts of end of the bridge tapioca flour, 7 parts of coconut powder,
2 parts of pine needle meal, 2 parts of sargassum powder, 0.6 part of vitamin A, 0.2 part of vitamin C and 0.5 part of vitamin E are fed 1 time for every 3 days,
Each feeding volume is the 2% of parent population weight；
(4) the sexually matured judgement of parent population
A, maturity inspection is carried out to female parent population weekly；Appearance upper abdomen protuberance, gonopore is micro- red, i.e., attainability at
It is ripe；
B, maturity inspection is carried out to male parent population weekly；Gonopore is micro- red, gently presses abdomen, can squeeze out milky essence
Liquid reaches sexal maturity；
(5) artificial insemination
Female epinephelus fuscoguttatus ovum and the big spot grouper sperm of the blue body of male are acquired respectively；Into the container for filling ovum
Clean seawater is added, amount of water is identical as semen volume, on one side plus seawater while be gently mixed, add the big spot lithosporic milt of blue body
Liquid, sperm additional amount are per kilogram ovum 1.3mL sperm, mix, and vibratory stimulation 10 seconds under the conditions of 50Hz, stand 2min,
With seawater flushing fertilized eggs, the seawater salinity is 28~31；
(6) hatching of fertilized eggs
It will be put into hatching barrel and hatch by step (5) processed fertilized eggs, incubating oosperm condition is dissolved oxygen in water
For 5~8mg/L, 25~28 DEG C of water temperature, salinity 28~31, pH7.0~8.0, hatching density is about 3,000,000 fertilized eggs/m3, often
Change within 3 hours that water is primary, each quantity of exchanged water is the 50% of hatching barrel volume, obtains prelarva after hatching.
Embodiment 3
A kind of artificial fecundation method of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body, comprising the following steps:
(1) Juvenile stage:
A, female parent: select 5 healthy epinephelus fuscoguttatus more than age as female parent；
B, male parent: select the blue big spot grouper of body of 7 health more than age as male parent；
(2) epinephelus fuscoguttatus parent culture is strengthened:
A, nurturing an environment: selecting outdoor pond, and 3~4m of the depth of water cultivates density about 250 tails/mu, female-male proportion 1:0.7；
B, feed A and feed B are fed,
The feed A by weight, including following component: 5 parts of mackerel, 11 parts of saury, 5 parts of spiral shell and Carnis Limax
It 2 parts, feeds daily 5 times, each feeding volume is the 1.3% of fish body weight
The feed B is man-made feeds, by weight, including following component: 7 parts of chicken droppings cane leaves powder, salt algae powder 0.9
Part splits 4 parts of pot algae powder and 0.6 part of vitamin E, feeds 1 time within every 3 days, and the feed B feeding volume is the 1.1% of fish body weight；
(3) cultivation of Lan Shen great spot grouper parent is strengthened
A, nurturing an environment: selecting outdoor pond, and 5~10m of the depth of water cultivates 60~75 tails of density/mu, female-male proportion 1:(2
~3)；
B, feed C and feed D are fed,
The feed C by weight, including following component: 11 parts of black cloth fish, 7 parts of Spanish mackerel, 4 parts of oyster and allicin
It 0.4 part, feeds daily 2 times, each feeding volume is the 1.5% of parent population weight；
The feed D is man-made feeds, by weight, including following component: 87 parts of end of the bridge tapioca flour, 5 parts of coconut powder,
1.2 parts of pine needle meal, 5 parts of sargassum powder, 0.4 part of vitamin A, 0.25 part of vitamin C and 0.4 part of vitamin E are fed for every 2 days
1 time, each feeding volume is the 2% of parent population weight；
(4) the sexually matured judgement of parent population
A, maturity inspection is carried out to female parent population weekly；Appearance upper abdomen protuberance, gonopore is micro- red, i.e., attainability at
It is ripe；
B, maturity inspection is carried out to male parent population weekly；Gonopore is micro- red, gently presses abdomen, can squeeze out milky essence
Liquid reaches sexal maturity；
(5) artificial insemination
Female epinephelus fuscoguttatus ovum and the big spot grouper sperm of the blue body of male are acquired respectively；Into the container for filling ovum
Clean seawater is added, amount of water is identical as semen volume, on one side plus seawater while be gently mixed, add the big spot lithosporic milt of blue body
Liquid, sperm additional amount are per kilogram ovum 1.2mL sperm, mix, and vibratory stimulation 20 seconds under the conditions of 40Hz, stand 2min,
With seawater flushing fertilized eggs, the seawater salinity is 28~31；
(6) hatching of fertilized eggs
It will be put into hatching barrel and hatch by step (5) processed fertilized eggs, incubating oosperm condition is dissolved oxygen in water
For 5~8mg/L, 25~28 DEG C of water temperature, salinity 28~31, pH7.0~8.0, hatching density is about 2,900,000 fertilized eggs/m3, often
Change within 2.5 hours that water is primary, each quantity of exchanged water is the 40% of hatching barrel volume, obtains prelarva after hatching.
Comparative example 1
The difference of this comparative example and embodiment 3 is, in step (2), saury replaces with decapterus maruadsi in the feed A,
And spiral shell and Carnis Limax are not contained；The feed B does not contain chicken droppings cane leaves powder.
Comparative example 2
The difference of this comparative example and embodiment 3 is, in step (2), the feed A by weight, including with the following group
Part: 10 parts of mackerel, 5 parts of saury, 0.5 part of spiral shell and 5 parts of Carnis Limax；The feed B by weight, including with the following group
Part: 2 parts of chicken droppings cane leaves powder, splits 1 part of pot algae powder and 1 part of vitamin E at 2 parts of salt algae powder.
Comparative example 3
The difference of this comparative example and embodiment 3 is, in step (3), black cloth fish and Spanish mackerel are replaced in the feed C
For mackerel.
Comparative example 4
The difference of this comparative example and embodiment 3 is, in step (3), the feed C includes following component: black cloth fish 5
Part, 10 parts of Spanish mackerel, 1 part of oyster and 1 part of allicin；The feed D includes following component: 5 parts of end of the bridge tapioca flour, coconut powder 1
Part, 3 parts of pine needle meal, 1 part of sargassum powder, 0.1 part of vitamin A, 0.1 part of vitamin C and 0.4 part of vitamin E.
Comparative example 5
The difference of this comparative example and embodiment 3 is, in step (5), does not carry out vibratory stimulation.
Comparative example 6
The difference of this comparative example and embodiment 3 is, in step (5), the vibration frequency is 100Hz, vibratory stimulation 1
Minute.
Egg laying amount, rate of fertilization, the hatching rate for measuring the various embodiments described above and comparative example respectively, as a result see the table below:
Above-described embodiment 1~3 the result shows that, using mating system of the present invention, the egg laying amount of parent population is high, and rate of fertilization is reachable
To 98.3%, the hatching rate of fertilized eggs can reach 95.7%.
Wherein, comparative example 1~2 and embodiment 3 compare, it is seen that the present invention feeds feed A for epinephelus fuscoguttatus and combines feeding
Expect B, influences egg laying amount, rate of fertilization, hatching rate.Using feed A combination feed B of the invention, egg laying amount, rate of fertilization are significantly improved
And hatching rate.
Comparative example 3~4 and embodiment 3 compare, it is seen that the present invention is directed to the blue big spot grouper of body and feeds feed C combination feed
D influences rate of fertilization, hatching rate.Using feed C combination feed D of the invention, rate of fertilization and hatching rate are significantly improved.
Comparative example 5~6 and embodiment 3 compare, and in fertilization process, the present invention uses vibratory stimulation, significantly improve fertilization
Rate, and Fertilized quality is improved, improve hatching rate.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. the artificial fecundation method of a kind of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body, it is characterised in that: including following
Step:
(1) Juvenile stage
A, female parent: select 5 healthy epinephelus fuscoguttatus more than age as female parent；
B, male parent: select the blue big spot grouper of body of 7 health more than age as male parent；
(2) epinephelus fuscoguttatus parent culture is strengthened
A, nurturing an environment: selecting outdoor pond, and 3~4m of the depth of water cultivates 200~300 tails of density/mu, female-male proportion 1:(0.5
~0.8)；
B, feed A and feed B are fed,
The feed A by weight, including following component: 2~6 parts of mackerel, 8~15 parts of saury, 3~8 parts of spiral shell and
It 1~3 part of Carnis Limax, feeds daily 3~5 times, each feeding volume is the 0.8~1.5% of fish body weight；
The feed B is man-made feeds, by weight, including following component: 5~10 parts of chicken droppings cane leaves powder, salt algae powder 0.7~
1.2 parts, split 3~6 parts of pot algae powder and 0.5~0.8 part of vitamin E, feed 1 time within every 2~3 days, the feed B feeding volume be fish
The 0.9~1.2% of body weight；
(3) cultivation of Lan Shen great spot grouper parent is strengthened
A, nurturing an environment: selecting outdoor pond, 5~10m of the depth of water, cultivates 60~75 tails of density/mu, female-male proportion be 1:(2~
3)；
B, feed C and feed D are fed,
The feed C by weight, including following component: 8~14 parts of black cloth fish, 5~8 parts of Spanish mackerel, 3~5 parts of oyster and
It 0.3~0.5 part of allicin, feeds daily 1~2 time, each feeding volume is the 1~2% of parent population weight；
The feed D is man-made feeds, by weight, including following component: 80~90 parts of end of the bridge tapioca flour, coconut powder 4~7
Part, 1~2 part of pine needle meal, 2~8 parts of sargassum powder, 0.2~0.6 part of vitamin A, 0.2~0.3 part of vitamin C and vitamin E
It 0.3~0.5 part, feeds 1 time within every 2~3 days, each feeding volume is the 2~3% of parent population weight；
(4) the sexually matured judgement of parent population
A, maturity inspection is carried out to female parent population weekly；Appearance upper abdomen protuberance, gonopore is micro- red, that is, can reach sexal maturity；
B, maturity inspection is carried out to male parent population weekly；Gonopore is micro- red, gently presses abdomen, can squeeze out milky sperm, i.e.,
Reach sexal maturity；
(5) artificial insemination
Female epinephelus fuscoguttatus ovum and the big spot grouper sperm of the blue body of male are acquired respectively；It is added into the container for fill ovum
Clean seawater, on one side plus seawater while be gently mixed, add the big spot grouper sperm of blue body, mix, and in 30~50Hz item
Vibratory stimulation 10~30 seconds under part stand 1~2min, with seawater flushing fertilized eggs；
(6) hatching of fertilized eggs
To be put into hatching barrel and hatch by step (5) processed fertilized eggs, incubating oosperm condition be dissolved oxygen in water be 5~
8mg/L, 25~28 DEG C of water temperature, salinity 28~31, pH7.0~8.0 obtain prelarva after hatching.
2. the artificial fecundation method of a kind of epinephelus fuscoguttatus according to claim 1 and the big spot grouper cenospecies of blue body,
It is characterized by: in step (2), the feed A by weight, including following component: 5 parts of mackerel, 11 parts of saury, river snail
5 parts and 2 parts of Carnis Limax of meat.
3. the artificial fecundation method of a kind of epinephelus fuscoguttatus according to claim 2 and the big spot grouper cenospecies of blue body,
It is characterized by: the feed A is fed 5 times daily in step (2), each feeding volume is the 1.3% of fish body weight.
4. the artificial propagation side of a kind of epinephelus fuscoguttatus according to claim 1 or 2 and the big spot grouper cenospecies of blue body
Method, it is characterised in that: in step (2), the feed B is cubed feed, by weight, including following component: chicken droppings cane leaves
7 parts of powder, splits 4 parts of pot algae powder and 0.6 part of vitamin E at 0.9 part of salt algae powder.
5. the artificial fecundation method of a kind of epinephelus fuscoguttatus according to claim 1 and the big spot grouper cenospecies of blue body,
It is characterized by: in step (3), the feed C by weight, including following component: 11 parts of black cloth fish, 7 parts of Spanish mackerel, male
4 parts and 0.4 part of allicin of oyster.
6. the artificial propagation side of a kind of epinephelus fuscoguttatus and the big spot grouper cenospecies of blue body according to claim 1 or 5
Method, it is characterised in that: in step (3), the feed D by weight, including following component: 87 parts of end of the bridge tapioca flour, coconut
5 parts of powder, 1.2 parts of pine needle meal, 5 parts of sargassum powder, 0.4 part of vitamin A, 0.25 part of vitamin C and 0.4 part of vitamin E.
7. the artificial fecundation method of a kind of epinephelus fuscoguttatus according to claim 1 and the big spot grouper cenospecies of blue body,
It is characterized by: sperm additional amount is per kilogram ovum 1.1~1.3mL sperm in step (5)；Seawater salinity is 28~31, sea
Water amount of water is 0.9~1.1 times of semen volume.
8. the artificial propagation side of a kind of epinephelus fuscoguttatus according to claim 1 or claim 7 and the big spot grouper cenospecies of blue body
Method, it is characterised in that: in step (5), the vibration frequency is 47Hz, and the vibratory stimulation time is 20 seconds.
9. the artificial fecundation method of a kind of epinephelus fuscoguttatus according to claim 1 and the big spot grouper cenospecies of blue body,
It is characterized by: hatching density is 280~3,000,000 fertilized eggs/m in step (6)3。
10. the artificial fecundation method of a kind of epinephelus fuscoguttatus according to claim 1 and the big spot grouper cenospecies of blue body,
It is characterized by: in step (6), change within every 2~3 hours that water is primary, each quantity of exchanged water is the 30~50% of hatching barrel volume.
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| CN111183934A (en) * | 2020-02-27 | 2020-05-22 | 海南晨海水产有限公司 | Outdoor artificial breeding method for acerola |
| CN112106698A (en) * | 2020-08-25 | 2020-12-22 | 中国水产科学研究院黄海水产研究所 | Method for breeding new germplasm of growth stress-resistant hybrid dominant grouper |
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Denomination of invention: Artificial propagation method of hybrid of brown spotted grouper and blue body grouper Effective date of registration: 20210928 Granted publication date: 20210604 Pledgee: CITIC Bank Haikou branch Pledgor: HAINAN CHENHAI AQUATIC Co.,Ltd. Registration number: Y2021980010243 |