CN110133119B - Detection method of L-alpha-glycerophosphorylcholine related substance - Google Patents
Detection method of L-alpha-glycerophosphorylcholine related substance Download PDFInfo
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- CN110133119B CN110133119B CN201910290607.0A CN201910290607A CN110133119B CN 110133119 B CN110133119 B CN 110133119B CN 201910290607 A CN201910290607 A CN 201910290607A CN 110133119 B CN110133119 B CN 110133119B
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Abstract
The invention relates to a method for detecting related substances in chemically synthesized L-alpha-glycerophosphorylcholine by liquid chromatography. Wherein the chromatographic column is a polar silica gel column or other equivalent chromatographic columns; the mobile phase is methanol-buffered salt solution, acetonitrile-buffered salt solution or methanol-acetonitrile-buffered salt solution and contains additives. The method has the advantages of simple operation, good specificity, precision and accuracy, and good durability, and is suitable for inspecting and controlling the quality of related substances in chemically synthesized L-alpha-glycerophosphorylcholine.
Description
Technical Field
The invention belongs to the field of analysis and detection, and particularly relates to a method for detecting related substances such as phosphoglyceride, choline and phosphorylcholine in L-alpha-glycerophosphorylcholine (hereinafter, glycerophosphorylcholine and GPC) by liquid chromatography.
Background
Glycerophosphorylcholine (GPC, structure shown as the following formula) is a product of completely hydrolyzing two fatty acyl groups on Phosphatidylcholine (PC) molecule, is a water-soluble small molecule substance endogenous to human body, and is also an important biosynthetic precursor of neurotransmitter Acetylcholine (Acetylcholine).
The conventional industrialized GPC preparation methods include a phosphatidylcholine hydrolysis method, a total chemical synthesis method and the like. GPC produced by total chemical synthesis produces some relevant impurities such as phosphoglycerides (degradation products), choline (starting material, degradation products), and phosphorylcholine (the form of starting material present in the product, degradation products), and possibly unknown impurities. In order to ensure the purity and quality safety of GPC products, related substances in GPC, including phosphoglyceride, choline, phosphorylcholine and other impurities, need to be detected.
Disclosure of Invention
Solves the technical problem
GPC and related substances, namely phosphoglyceride, choline and phosphorylcholine, are water-soluble compounds with large polarity, have no ultraviolet absorption and can not excite fluorescence, so that the development of a detection method is difficult. At present, no detection method for related substances in total chemical synthesis GPC is reported.
In order to solve the above problems, it is an object of the present invention to provide a high performance liquid chromatography method which has a good retention property, satisfies a required degree of separation between adjacent peaks, has good symmetry of peak shape, and can be used for quantitative measurement of related substances, i.e., phosphoglyceride (code: a), choline (code: b), and phosphorylcholine (code: c), in GPC, thereby ensuring the quality of GPC products.
Technical scheme
In order to realize the purpose, the invention adopts the technical scheme that:
a method for detecting related substances such as phosphoglyceride, choline and phosphorylcholine in glycerophosphorylcholine by liquid chromatography, wherein the used chromatographic column is a polar silica gel column or other equivalent chromatographic columns; the mobile phase is methanol-buffered salt solution, acetonitrile-buffered salt solution or methanol-acetonitrile-buffered salt solution and contains additives.
The detector used in the method is a refractive index detector, an evaporative light scattering detector or a mass spectrometry detector.
The liquid chromatography comprises conventional liquid chromatography or ultra-high performance liquid chromatography.
Preferably, the chromatographic column is a polar column using silica gel or bonded silica gel as a filler.
Preferably, the column temperature of the chromatographic column is 25-50 ℃, more preferably 40 ℃.
Preferably, the buffer salt is one or more of a positive ion pairing agent (such as tetra-n-butyl ammonium bromide and the like), a negative ion pairing agent (such as sodium heptanesulfonate, sodium octanesulfonate, sodium hexanesulfonate and the like), a formate (such as ammonium formate), an acetate (such as ammonium acetate), a phosphate (such as sodium dihydrogen phosphate) and the like.
Preferably, the mobile phase is a methanol-ammonium formate aqueous solution system, wherein the volume ratio of methanol to the mobile phase is 50-80%, more preferably 65%; the concentration of ammonium formate in the aqueous ammonium formate solution is 10-100mM, more preferably 20 mM.
Preferably, the additive is one or more of formic acid, formate, acetic acid, acetate, phosphoric acid and phosphate, more preferably phosphoric acid or phosphate, and the mass fraction of the additive in the water phase (i.e. the buffer salt solution) in the mobile phase is 0.05-2%. More preferably, the phosphate is selected from one or more of sodium dihydrogen phosphate and potassium dihydrogen phosphate.
Preferably, the mobile phase flow rate is 0.1-2.0 mL/min.
Specifically, the method for detecting related substances, namely phosphoglyceride, choline and phosphorylcholine in glycerophosphorylcholine by using the liquid chromatography comprises the following steps:
step 1: preparation of a blank solution: taking a methanol water solution as a blank solution;
step 2: preparation of a mobile phase system: preparing an aqueous solution containing 20mM ammonium formate as an aqueous phase, adding phosphate accounting for 0.1% of the mass fraction of the aqueous phase, measuring methanol as an organic phase, wherein the volume of the organic phase and the aqueous phase is 65:35, and uniformly mixing the organic phase and the aqueous phase;
and step 3: preparation of related substance control solution: respectively weighing related substances such as phosphoglyceride, choline and phosphorylcholine, precisely weighing, adding methanol water solution for dissolving, and respectively preparing related substance control solution containing 0.4mg +/-0.02 mg per 1 mL;
and 4, step 4: preparing a test solution: accurately weighing about 0.4g +/-0.05 g of a glycerol phosphorylcholine sample to be determined, adding methanol aqueous solution to dissolve the glycerol phosphorylcholine sample to be determined to prepare a sample solution containing about 80mg of the glycerol phosphorylcholine sample to be determined and various related substances with unknown content per 1 mL;
respectively and precisely measuring 10 mu L of each of the blank solution prepared in the step 1, the related substance reference solution prepared in the step 3 and the test solution prepared in the step 4, detecting by using a liquid chromatography, recording a chromatogram, and calculating the contents of related substances, namely phosphoglyceride, choline and phosphorylcholine in a glycerophosphorylcholine sample to be measured according to an external standard method.
The calculation formula is as follows:
wherein, CRThe concentration of the substance to be detected in the related substance control solution is mg/mL;
Csthe concentration of the glycerophosphorylcholine sample in the test solution is mg/mL;
AR，AXpeak areas, mV, of the substance to be detected in the related substance control solution and the test solution are respectively;
preferably, the volume ratio of methanol to water in the methanol aqueous solution in step 1, step 3 and step 4 is 65: 35.
Advantageous effects
Proved by methodology verification of a specificity test, a linear test, a precision test and an accuracy test, the method for detecting the GPC related substance provided by the invention has the following advantages:
(1) the main component GPC can be well separated from related substances and all related substances.
(2) The method has good specificity and durability.
(3) The method has good precision.
(4) GPC and related substances show good linear relation in a certain concentration range.
(5) The method has good accuracy.
(6) Simultaneously realizes GPC and better peak shapes of related substances a, b and c.
Drawings
FIG. 1 shows a detection spectrum obtained by the method of the present invention (curve 1 is a blank solution chromatogram; curve 2 is a typical separation chromatogram of GPC and related substances, peak a is phosphoglyceride, peak b is choline, peak c is phosphorylcholine, peak d is GPC, and peak x is unknown impurity).
Detailed Description
The following embodiments of the present invention are described in detail, and it should be noted that the following embodiments are exemplary only, and are not to be construed as limiting the present invention. In addition, the reagents used in the examples are all commercially available, if not explicitly stated.
Example (b): this example provides the detection of GPC-related substances using the method of the invention.
Instrument and chromatographic conditions: shimazu LC-20 high performance liquid chromatograph; the chromatographic column is a polar silica gel column; the column temperature was 40 ℃; the method comprises the following steps of mixing methanol: a 20mM ammonium formate solution (65:35, v: v) containing 0.1% phosphate as a mobile phase; detecting with high performance chromatography liquid system with sample volume of 10 μ L; the flow rate is 1.0 mL/min; using a differential refractive detector; the detector temperature was 40 ℃.
Experimental procedure
Step 1: preparation of a blank solution: taking methanol: a water (65:35, v: v) mixed solution was used as a blank solution.
Step 2: preparation of a mobile phase system: measuring a proper amount of deionized water, adding a precisely-weighed proper amount of ammonium formate to prepare an aqueous solution containing 20mM ammonium formate as a water phase, adding 0.1% of sodium dihydrogen phosphate (the mass fraction of the sodium dihydrogen phosphate in the water phase is 0.1%), measuring a proper amount of methanol as an organic phase, wherein the ratio of the organic phase to the water phase is 65:35(v: v), uniformly mixing, filtering and performing ultrasonic treatment.
And step 3: preparation of individual related substance control stock solutions: respectively taking related substances of phosphoglyceride (a), choline (b) and phosphorylcholine (c), precisely weighing, adding methanol: water (65:35, v: v) was dissolved to prepare individual control stock solutions containing about 2mg of the relevant substance per 1mL, respectively.
And 4, step 4: preparation of related substance control solution: taking 1mL of each reference substance stock solution of the single related substances prepared in the step 3, placing the reference substances in the same 5mL volumetric flask, adding methanol: the resulting solution was dissolved in water (65:35, v: v) to prepare a control solution containing about 0.4mg of each relevant substance per 1 mL.
And 5: preparing a mixed solution: 1mL of each of the individual reference substance stock solutions prepared in step 3 was placed in the same 5mL volumetric flask, and about 0.4g of GPC sample (without impurities a, b, and c) was precisely weighed, and mixed with methanol: dissolving in water (65:35, v: v) to obtain a mixed solution containing about 80mg of the main component and 0.4mg of each related substance per 1mL (each related substance accounts for about 0.5% of the main component).
Precisely measuring 10 μ L of each of the blank solution prepared in step 1 and the mixed solution prepared in step 5, measuring by sample introduction, and recording chromatogram as shown in FIG. 1.
The chromatographic peak sequence is phosphoglyceride, choline, GPC and phosphorylcholine. Relative retention time of phosphoglyceride, calculated as GPC retention time, was 0.4; relative retention time of choline was 0.9; the relative retention time of phosphorylcholine was 1.3. In addition, at least one unknown impurity was present before the choline peak, with a relative retention time of 0.6.
The test result shows that the blank solvent peak has no interference to the detection of GPC and related substances, the separation degree between GPC and the related substances and between the related substances is more than 1.5, and the peak shape of the related substances is good.
Precisely measuring 10 μ L of the related substance control solution prepared in step 4, carrying out parallel sample injection for 3 times on the first day, the second day and the third day, and determining according to the method. The following table shows the Relative Standard Deviation (RSD) of peak areas for each of the relevant substances. The results show that the RSD of the peak area of each related substance is less than 2 percent, which indicates that the precision of the method is good.
| Related substances | a | b | c |
| Peak area RSD% | 1.67 | 0.99 | 1.76 |
And (4) comparing the measurement result of the mixed solution prepared in the step (5) with the measurement result of the reference solution prepared in the step (4), and inspecting the recovery rate of each related substance. The recovery rate of each related substance is 95-108%, which shows that the method has good accuracy.
The recovery rate calculation formula is as follows:
wherein Ax, ARRespectively the peak area of the substance to be detected in the mixed solution and the corresponding substance to be detected in the reference substance solution
Peak area of the material, mV;
Cx、CRthe concentration of the substance to be detected in the mixed solution and the corresponding concentration of the substance to be detected in the related substance reference solution are respectively,
mg/mL。
and (4) diluting the reference substance solution prepared in the step (4) step by step, feeding the diluted reference substance solution for measurement, taking the signal-to-noise ratio of 2-3 as a detection limit, and calculating the detection limit of each related substance as shown in the table. The method has higher sensitivity and can meet the detection requirement.
In conclusion, the method for detecting related substances such as phosphoglyceride, choline and phosphorylcholine in glycerophosphorylcholine by liquid chromatography provided by the invention has the advantages of simple and convenient operation, good specificity, strong precision and accuracy and high sensitivity, and is suitable for checking and controlling the quality of related substances in glycerophosphorylcholine.
Claims (4)
1. A method for detecting related substances of phosphoglyceride, choline and phosphorylcholine in glycerophosphorylcholine by liquid chromatography is characterized in that: the chromatographic column is a polar silica gel column; the mobile phase is a methanol-ammonium formate aqueous solution system, and the volume ratio of methanol to the mobile phase is 65 percent; the concentration of ammonium formate in the aqueous ammonium formate solution was 20mM, and the mobile phase contained 0.1% by mass of sodium dihydrogen phosphate in the buffered salt solution.
2. The method for detecting related substances, namely phosphoglyceride, choline and phosphorylcholine, in glycerophosphorylcholine by liquid chromatography according to claim 1, wherein: the detector used is a differential refraction detector, an evaporative light scattering detector or a mass spectrometry detector.
3. The method for detecting related substances, namely phosphoglyceride, choline and phosphorylcholine, in glycerophosphorylcholine by liquid chromatography as claimed in claim 1, comprising the following steps:
step 1: preparation of a blank solution: taking a methanol water solution as a blank solution;
step 2: preparation of a mobile phase system: preparing an aqueous solution containing 20mM ammonium formate as an aqueous phase, adding phosphate accounting for 0.1% of the mass fraction of the aqueous phase, measuring methanol as an organic phase, wherein the volume of the organic phase and the aqueous phase is 65:35, and uniformly mixing the organic phase and the aqueous phase;
and step 3: preparation of related substance control solution: respectively weighing related substances such as phosphoglyceride, choline and phosphorylcholine, precisely weighing, adding methanol water solution for dissolving, and respectively preparing related substance reference substance solutions containing 0.4mg +/-0.02 mg per 1 mL;
and 4, step 4: preparing a test solution: accurately weighing about 0.4g +/-0.05 g of a glycerol phosphorylcholine sample to be determined, adding methanol aqueous solution to dissolve the glycerol phosphorylcholine sample to be determined to prepare a sample solution containing about 80mg of the glycerol phosphorylcholine sample to be determined and various related substances with unknown content per 1 mL;
respectively and precisely measuring 10 mu L of each of the blank solution prepared in the step 1, the related substance reference solution prepared in the step 3 and the test solution prepared in the step 4, detecting by using a liquid chromatography, recording a chromatogram, and calculating the contents of related substances, namely phosphoglyceride, choline and phosphorylcholine, in a glycerophosphorylcholine sample to be measured according to an external standard method;
the calculation formula is as follows: content% = (CR × AX)/(Cs × AR) × 100%
Wherein CR is the concentration of the substance to be detected in the related substance control solution, mg/mL;
cs is the concentration of the glycerophosphorylcholine sample in the test solution, mg/mL;
AR and AX are peak areas, mV, of the substance to be detected in the related substance control solution and the test solution respectively.
4. The method for detecting related substances, i.e., phosphoglyceride, choline and phosphorylcholine, in glycerophosphorylcholine by liquid chromatography according to claim 3, wherein: the volume ratio of methanol to water in the methanol aqueous solution in the steps 1, 3 and 4 is 65: 35.
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