CN110025826B - Guided bone tissue regeneration membrane, preparation method and application - Google Patents
Guided bone tissue regeneration membrane, preparation method and application Download PDFInfo
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Abstract
The invention provides a guided bone tissue regeneration membrane, a preparation method and application. The preparation method of the guided bone tissue regeneration membrane comprises the following steps: a. mixing a medical degradable material with an organic solvent to prepare a polymer solution; b. uniformly mixing the polymer solution prepared in the step a with a prepared dexamethasone solution to prepare an electrostatic spinning solution; c. carrying out electrostatic spinning by adopting the electrostatic spinning solution prepared in the step b to obtain an electrostatic spinning film; d. c, drying the electrostatic spinning membrane prepared in the step c in sequence to remove the solvent, and performing surface functionalization treatment on the dopamine to obtain a functionalized membrane; e. d, soaking the functional membrane prepared in the step d in a pre-prepared antibacterial polypeptide solution and then taking out the functional membrane; f. and e, washing the film prepared in the step e with distilled water and drying. The guided bone tissue regeneration membrane provided by the invention has the beneficial effects of degradability, strong biocompatibility, promotion of osteogenic differentiation and growth, antibiosis and the like.
Description
Technical Field
The application relates to the technical field of medical materials, in particular to a degradable antibacterial polypeptide modified guided bone tissue regeneration membrane, a preparation method and application.
Background
The Guided Bone tissue Regeneration (GBR) technology is one of the most frequently used and most effective Bone augmentation techniques in clinic, and the Guided Bone tissue Regeneration membrane has good Bone tissue Regeneration capability and is widely applied to the fields of Bone defect treatment, oral craniomaxillofacial defect repair and implantation and the like.
The advent of electrospun membranes effectively ameliorated some of the deficiencies of conventional GBR membranes, including: the three-dimensional space structure is lacked, and the tiled film can not provide enough growth, extension and proliferation space for the growth of osteocytes; the versatility of the material cannot be achieved.
Moreover, the medical degradable material has better biocompatibility and plasticity, which provides a foundation for further clinical application of the bone implant material and realization of multi-functionalization of the guided bone tissue regeneration membrane, but the simple degradable medical implant material composite electrostatic spinning membrane has the defects of weaker bone guiding performance, lower cell response and the like in the tissue repair application, and the clinical application of the electrostatic spinning membrane is limited to a great extent; moreover, in clinical studies, it has been found that extensive bone tissue damage is generally accompanied by a threat of inflammation, preventing further bone tissue repair.
In view of the above, there is a need to provide a guided bone tissue regeneration membrane modified by osteogenic active substances, antibacterial agents, etc., a preparation method and applications thereof, so as to solve the above problems.
Disclosure of Invention
The invention aims to provide a preparation method of a guided bone tissue regeneration membrane, which is used for preparing the guided bone tissue regeneration membrane with medical degradable materials, medical antibacterial drugs and osteogenic active substances and has the advantages of bone regeneration promotion, antibiosis and good biocompatibility.
In order to achieve one of the above objects, an embodiment of the present invention provides a method for preparing a guided bone tissue regeneration membrane, comprising the steps of:
a. mixing a medical degradable material with an organic solvent to prepare a polymer solution;
b. uniformly mixing the polymer solution prepared in the step a with a prepared dexamethasone solution to prepare an electrostatic spinning solution;
c. carrying out electrostatic spinning by adopting the electrostatic spinning solution prepared in the step b to obtain an electrostatic spinning film;
d. c, drying the electrostatic spinning membrane prepared in the step c in sequence to remove the solvent, and performing surface functionalization treatment on the dopamine to obtain a functionalized membrane;
e. d, soaking the functional membrane prepared in the step d in a pre-prepared antibacterial polypeptide solution and then taking out the functional membrane;
f. and e, washing the film prepared in the step e with distilled water and drying.
As a further improvement of an embodiment of the present invention, in step a, the medical degradable material is at least one of polylactic acid-glycolic acid copolymer, polycaprolactone, polylactic acid, and poly β -hydroxybutyrate.
In a further improvement of an embodiment of the present invention, in step a, the organic solvent is at least one of trifluoroethanol, acetone, hexafluoroisopropanol, dimethylformamide and tetrahydrofuran.
In a further improvement of an embodiment of the present invention, in step a, the amount of the medical degradable material is 0.1 to 2g, and the amount of the organic solvent is 1 to 10 mL.
As a further improvement of one embodiment of the present invention, in step b, dexamethasone is dissolved in absolute ethanol and prepared to have a concentration of 10-6mol/L～10-9The preparation method comprises the following steps of preparing a dexamethasone solution in mol/L, wherein the dosage of the dexamethasone solution is 800-2000 mu L.
As a further improvement of an embodiment of the present invention, in step c, electrospinning is performed in an electrospinning device under the following conditions: the voltage is 5-30 kV, the receiving distance is 5-30 cm, and the sample introduction speed is 0.5-5 mL/h.
As a further improvement of one embodiment of the invention, in step d, dopamine is added into a Tris-HCl solution to prepare a dopamine solution with a pH value of 5-11 and a concentration of 0.1-50 mg/mL;
in the step d, the dopamine surface functionalization treatment process comprises the following steps: and (3) soaking the electrostatic spinning membrane in the dopamine solution under the condition of introducing oxygen, then taking out, washing with distilled water, and drying.
As a further improvement of one embodiment of the invention, in step e, the antibacterial polypeptide in the antibacterial polypeptide solution is Inverso-CysHHC10, HHC-36, Tet213 or c-hLF 1-11; the concentration of the antibacterial polypeptide solution is 0.1-10 mg/mL.
In order to achieve one of the above objects, an embodiment of the present invention further provides a guided bone tissue regeneration membrane prepared by the preparation method.
In order to achieve one of the above objects, an embodiment of the present invention further provides a use of the guided bone tissue regeneration membrane in bone repair.
Compared with the prior art, the invention has the beneficial effects that: the prepared guided bone tissue regeneration membrane has medical degradable materials, dexamethasone and antibacterial polypeptide, on one hand, the guided bone tissue regeneration membrane has good degradability and biocompatibility based on the medical degradable materials, and has a three-dimensional porous structure simulating an extracellular matrix, so that migration and proliferation of osteoblasts are facilitated; on the other hand, the addition of dexamethasone promotes osteogenic differentiation and improves osteogenic performance; on the other hand, based on the addition of the antibacterial polypeptide, the antibacterial polypeptide has low cytotoxicity and does not cause bacterial drug resistance, so that the threat of inflammation is reduced, and the further repair of bone tissues is promoted; finally, the adhesion effect of dopamine is adopted, the problem that the requirement of the antibacterial polypeptide on grafting conditions is high is solved, and the antibacterial polypeptide is fixed on the surface of the guided bone tissue regeneration membrane so as to keep the unique antibacterial effect of the guided bone tissue regeneration membrane.
Detailed Description
The present invention will be described in detail with reference to embodiments, but these embodiments do not limit the present invention, and the changes of reaction conditions, reactants or raw materials amount according to these embodiments are included in the protection scope of the present invention by those skilled in the art.
As mentioned in the background art, the medical degradable material has better biocompatibility and plasticity, but the pure degradable medical implant material composite electrostatic spinning membrane has the defects of weaker bone guiding performance, lower cell response and the like in the tissue repair application, thereby limiting the clinical application of the electrostatic spinning membrane to a great extent; in addition, in clinical research, large-area bone tissue damage is generally accompanied by the threat of inflammation and infection, and further repair of the bone tissue is prevented.
Therefore, through a great deal of research, the inventor of the present application provides a preparation method of a guided bone tissue regeneration membrane, and the guided bone tissue regeneration membrane prepared by the preparation method is modified and modified by adding osteogenic active substances, antibacterial drugs and the like on the basis of a medical degradable material electrostatic spinning membrane, so that the versatility is realized.
Specifically, dexamethasone (DEXAMethanesone, DEX) is an artificially synthesized glucocorticoid, and has pharmacological effects of anti-inflammation, anti-toxicity, anti-allergy, anti-rheumatism, etc., and also has effects of osteogenic differentiation. Through the research of the inventor, the dexamethasone is added into the medical degradable material matrix as an osteogenesis active substance through an electrostatic spinning technology, and the prepared electrostatic spinning membrane has a good effect of promoting bone growth.
Antimicrobial polypeptides (AMPs), as a novel antimicrobial drug, consisting of amino acids, have a broad spectrum of antimicrobial activity and low cytotoxicity, and in particular AMPs, due to its specific antimicrobial mechanism, do not cause bacterial resistance and are biocompatible in various models; however, the grafting conditions for immobilizing the antibacterial polypeptide on the surface of the material are highly required. Through the research of the inventor, the antibacterial polypeptide can be effectively fixed on the surface of the guided bone tissue regeneration membrane in an auxiliary mode of dopamine coating adhesion, so that the unique antibacterial effect of the antibacterial polypeptide is kept, and the infection caused by the adhesion of bacteria on the surface of the guided bone tissue regeneration membrane is avoided.
The adhesion mode of the dopamine coating is roughly described in principle: dopamine is a molecule combining the functions of catechol and amine, and under oxidation and alkaline conditions, a thin attached polymer film (pDA) is deposited on the surface of different types of materials, and the pDA layer can spontaneously react with amino acid or amino groups on protein under mild conditions, so that the immobilization of antibacterial polypeptide is assisted.
Specifically, the preparation method of the guided bone tissue regeneration membrane provided by the invention comprises the following steps:
a. mixing the medical degradable material with an organic solvent to prepare a polymer solution.
The medical degradable material is a medical degradable high polymer material, and specifically is at least one of polylactic acid-glycolic acid copolymer, polycaprolactone, polylactic acid and poly beta-hydroxybutyrate.
The organic solvent is at least one of trifluoroethanol, acetone, hexafluoroisopropanol, dimethylformamide and tetrahydrofuran.
The dosage of the medical degradable material is 0.1-2 g, and the dosage of the organic solvent is 1-10 mL.
b. And c, uniformly mixing the polymer solution prepared in the step a with a prepared dexamethasone solution to prepare the electrostatic spinning solution.
The preparation process of the dexamethasone solution comprises the following steps: dissolving dexamethasone in anhydrous ethanol to obtain a solution with a concentration of 10- 6mol/L～10-9mol/L dexamethasone solution. By optimizing the concentration of the dexamethasone solution, the method is more beneficial to dexamethasone to realize the osteogenesis activity, so that the finally prepared guided bone tissue regeneration membrane has better osteogenesis differentiation promotion performance, and the problem that glucocorticoid-induced osteoporosis is caused by reduction or inhibition of osteogenesis differentiation due to overhigh concentration of the dexamethasone solution or the problem that osteogenesis differentiation cannot be effectively promoted due to overlow concentration of the dexamethasone solution is avoided.
The dosage of the dexamethasone solution is 800-2000 mu L.
c. Carrying out electrostatic spinning by adopting the electrostatic spinning solution prepared in the step b to obtain an electrostatic spinning film; specifically, electrospinning was carried out in an electrospinning apparatus under the following conditions: the voltage is 5-30 kV, the receiving distance is 5-30 cm, and the sample introduction speed is 0.5-5 mL/h; the obtained electrospun film has a three-dimensional structure.
d. And c, drying the electrostatic spinning membrane prepared in the step c in sequence to remove the solvent, and performing surface functionalization treatment on the dopamine to obtain the functionalized membrane.
The dopamine surface functionalization treatment process comprises the following steps: soaking the electrostatic spinning membrane in dopamine solution under the condition of oxygen supply, then taking out, washing with distilled water, and drying; the preparation process of the dopamine solution comprises the following steps: adding dopamine into a Tris-HCl solution to prepare a dopamine solution with a pH value of 5-11 and a concentration of 0.1-50 mg/mL.
e. And d, soaking the functional membrane prepared in the step d in a pre-prepared antibacterial polypeptide solution, and then taking out.
Wherein the antibacterial polypeptide in the antibacterial polypeptide solution is Inverso-CysHHC10, HHC-36, Tet213 or c-hLF 1-11; the concentration of the antibacterial polypeptide solution is 0.1-10 mg/mL.
f. And e, washing the film prepared in the step e with distilled water and drying.
The membrane for guiding bone tissue regeneration, which is prepared by the preparation method, has the advantages of medical degradable material, dexamethasone and antibacterial polypeptide, is degradable, has strong biocompatibility, can promote osteogenesis differentiation and growth, is antibacterial and the like, and can be well applied to bone repair. Specifically, on one hand, the bone tissue regeneration membrane has good degradability and biocompatibility based on a medical degradable material, and is guided to have a three-dimensional porous structure simulating an extracellular matrix, so that migration and proliferation of osteoblasts are facilitated; on the other hand, the addition of dexamethasone promotes osteogenic differentiation and improves osteogenic performance; on the other hand, based on the addition of the antibacterial polypeptide, the antibacterial polypeptide has low cytotoxicity and does not cause bacterial drug resistance, so that the threat of inflammation is reduced, and the further repair of bone tissues is promoted; finally, the adhesion effect of dopamine is adopted, the problem that the requirement of the antibacterial polypeptide on grafting conditions is high is solved, and the antibacterial polypeptide is fixed on the surface of the guided bone tissue regeneration membrane so as to keep the unique antibacterial effect of the guided bone tissue regeneration membrane.
The preparation process of the present invention will be further described with reference to specific examples.
The first embodiment is as follows:
2g of polycaprolactone is weighed, added into 10mL of hexafluoroisopropanol solvent and stirred uniformly on a magnetic stirrer to prepare a polymer solution.
Precisely weighing dexamethasone powder, dissolving in anhydrous ethanol to obtain a solution with a concentration of 10-7A mol/L dexamethasone solution; and adding 800 mu L of the dexamethasone solution into the polymer solution, and uniformly mixing to obtain the electrostatic spinning solution.
Adding the electrostatic spinning solution into an electrostatic spinning device for electrostatic spinning to obtain an electrostatic spinning film with a three-dimensional structure, wherein the electrostatic spinning conditions are as follows: the voltage is 20kV, the receiving distance is 30cm, the sample injection speed is 0.5mL/h, and the electrostatic spinning membrane is obtained and has the medical degradable material and dexamethasone.
Drying the electrospun membrane to remove solvent; then, soaking the electrostatic spinning membrane in a dopamine solution, and introducing oxygen into the dopamine solution for 1h while soaking; and then, taking the soaked electrostatic spinning membrane out of the dopamine solution, washing the electrostatic spinning membrane with distilled water for 2-3 times, and drying with a vacuum drying oven to obtain a functional membrane, wherein the functional membrane has the medical degradable material, dexamethasone and dopamine (coated on the surface of the membrane).
After the functionalized membrane is soaked in the antibacterial polypeptide solution, the functionalized membrane is taken out of the antibacterial polypeptide solution after reacting for a period of time, then the functionalized membrane is washed by distilled water for 2-3 times and is dried and stored, and finally the guided bone tissue regeneration membrane is prepared.
The preparation process of the dopamine solution comprises the following steps: Tris-HCl solution is prepared, pH value is adjusted to be 6.0, 400mg of dopamine is weighed and added into 10mL of Tris-HCl solution, and dopamine solution with concentration of 40mg/mL is prepared. The preparation process of the antibacterial polypeptide solution comprises the following steps: the antibacterial polypeptide selects Tet213, and Tet213 is prepared into a solution with the concentration of 0.5 mg/mL.
Example two:
weighing 1g of polycaprolactone and 1g of poly beta-hydroxybutyrate copolymer, adding the polycaprolactone and the poly beta-hydroxybutyrate copolymer into 10mL of trifluoroethanol solvent, and uniformly stirring the mixture on a magnetic stirrer to prepare a polymer solution.
Precisely weighing dexamethasone powder, dissolving in anhydrous ethanol to obtain a solution with a concentration of 10-8A mol/L dexamethasone solution; and adding 800 mu L of the dexamethasone solution into the polymer solution, and uniformly mixing to obtain the electrostatic spinning solution.
Adding the electrostatic spinning solution into an electrostatic spinning device for electrostatic spinning to obtain an electrostatic spinning film with a three-dimensional structure, wherein the electrostatic spinning conditions are as follows: the voltage is 18kV, the receiving distance is 20cm, the sample injection speed is 3.5mL/h, and the electrostatic spinning membrane is obtained and has the medical degradable material and dexamethasone.
Drying the electrostatic spinning membrane to remove the solvent; then, soaking the electrostatic spinning membrane in a dopamine solution, and introducing oxygen into the dopamine solution for 5 hours while soaking; and then, taking the soaked electrostatic spinning membrane out of the dopamine solution, washing the electrostatic spinning membrane with distilled water for 2-3 times, and drying with a vacuum drying oven to obtain a functional membrane, wherein the functional membrane has the medical degradable material, dexamethasone and dopamine (coated on the surface of the membrane).
After the functionalized membrane is soaked in the antibacterial polypeptide solution, the functionalized membrane is taken out of the antibacterial polypeptide solution after reacting for a period of time, then the functionalized membrane is washed by distilled water for 2-3 times and is dried and stored, and finally the guided bone tissue regeneration membrane is prepared.
The preparation process of the dopamine solution comprises the following steps: preparing a Tris-HCl solution, adjusting the pH value to 8.0, weighing 100mg of dopamine, adding the dopamine into 10mL of the Tris-HCl solution, and preparing the dopamine solution with the concentration of 10 mg/mL. The preparation process of the antibacterial polypeptide solution comprises the following steps: the antibacterial polypeptide is c-hLF1-11, and the c-hLF1-11 is prepared into a solution with the concentration of 2 mg/mL.
Example three:
0.5g of poly beta-hydroxybutyrate copolymer and 0.5g of polylactic acid-glycolic acid copolymer are weighed and added into 8mL of trifluoroethanol solvent, and the mixture is stirred uniformly on a magnetic stirrer to prepare polymer solution.
Precisely weighing dexamethasone powder, dissolving in anhydrous ethanol to obtain a solution with a concentration of 10-9A mol/L dexamethasone solution; and adding 800 mu L of the dexamethasone solution into the polymer solution, and uniformly mixing to obtain the electrostatic spinning solution.
Adding the electrostatic spinning solution into an electrostatic spinning device for electrostatic spinning to obtain an electrostatic spinning film with a three-dimensional structure, wherein the electrostatic spinning conditions are as follows: the voltage is 15kV, the receiving distance is 15cm, the sample injection speed is 2.5mL/h, and the electrostatic spinning membrane is obtained and has the medical degradable material and dexamethasone.
Drying the electrostatic spinning membrane to remove the solvent; then, soaking the electrostatic spinning membrane in a dopamine solution, and introducing oxygen into the dopamine solution for 12 hours while soaking at room temperature; and then, taking the soaked electrostatic spinning membrane out of the dopamine solution, washing the electrostatic spinning membrane with distilled water for 2-3 times, and drying with a vacuum drying oven to obtain a functional membrane, wherein the functional membrane has the medical degradable material, dexamethasone and dopamine (coated on the surface of the membrane).
After the functionalized membrane is soaked in the antibacterial polypeptide solution, the functionalized membrane is taken out of the antibacterial polypeptide solution after reacting for a period of time, then the functionalized membrane is washed by distilled water for 2-3 times and is dried and stored, and finally the guided bone tissue regeneration membrane is prepared.
The preparation process of the dopamine solution comprises the following steps: Tris-HCl solution is prepared, pH value is adjusted to be 9.0, 40mg of dopamine is weighed and added into 10mL of Tris-HCl solution, and dopamine solution with concentration of 4mg/mL is prepared. The preparation process of the antibacterial polypeptide solution comprises the following steps: the antibacterial polypeptide is Inverso-CysHHC10, and Inverso-CysHHC10 is prepared into a solution with the concentration of 5 mg/mL.
Example four:
0.5g of polycaprolactone and 0.5g of polylactic acid-glycolic acid copolymer are weighed, added into 5mL of trifluoroethanol solvent, and stirred uniformly on a magnetic stirrer to prepare polymer solution.
Precisely weighing dexamethasone powder, dissolving in anhydrous ethanol to obtain a solution with a concentration of 10-8A mol/L dexamethasone solution; and adding 1000 mu L of the dexamethasone solution into the polymer solution, and uniformly mixing to obtain the electrostatic spinning solution.
Adding the electrostatic spinning solution into an electrostatic spinning device for electrostatic spinning to obtain an electrostatic spinning film with a three-dimensional structure, wherein the electrostatic spinning conditions are as follows: the voltage is 10kV, the receiving distance is 20cm, the sample injection speed is 3.5mL/h, and the electrostatic spinning membrane is obtained and has the medical degradable material and dexamethasone.
Drying the electrospun membrane to remove solvent; then, soaking the electrostatic spinning membrane in a dopamine solution, and introducing oxygen into the dopamine solution for 24 hours while soaking at room temperature; and then, taking the soaked electrostatic spinning membrane out of the dopamine solution, washing the electrostatic spinning membrane with distilled water for 2-3 times, and drying with a vacuum drying oven to obtain a functional membrane, wherein the functional membrane has the medical degradable material, dexamethasone and dopamine (coated on the surface of the membrane).
After the functionalized membrane is soaked in the antibacterial polypeptide solution, the functionalized membrane is taken out of the antibacterial polypeptide solution after reacting for a period of time, then the functionalized membrane is washed by distilled water for 2-3 times and is dried and stored, and finally the guided bone tissue regeneration membrane is prepared.
The preparation process of the dopamine solution comprises the following steps: Tris-HCl solution is prepared, the pH value is adjusted to 8.5, 20mg of dopamine is weighed and added into 10mL of Tris-HCl solution, and the dopamine solution with the concentration of 2mg/mL is prepared. The preparation process of the antibacterial polypeptide solution comprises the following steps: HHC-36 is selected as the antibacterial polypeptide, and the HHC-36 is prepared into a solution with the concentration of 0.5 mg/mL.
It should be understood that although the present description refers to embodiments, not every embodiment contains only a single technical solution, and such description is for clarity only, and those skilled in the art should make the description as a whole, and the technical solutions in the embodiments can also be combined appropriately to form other embodiments understood by those skilled in the art.
The above list of details is only for the concrete description of the feasible embodiments of the present application, they are not intended to limit the scope of the present application, and all equivalent embodiments or modifications that do not depart from the technical spirit of the present application are intended to be included within the scope of the present application.
Claims (9)
1. A preparation method of a guided bone tissue regeneration membrane is characterized by comprising the following steps:
a. mixing a medical degradable material with an organic solvent to prepare a polymer solution;
b. mixing the polymer solution prepared in the step a with a pre-prepared concentration of 10%-6mol/L～10-9Uniformly mixing a mol/L dexamethasone solution to prepare an electrostatic spinning solution; wherein the medical degradable material: the organic solvent is: the dosage ratio of the dexamethasone solution is 0.1-2 g: 1-10 mL: 800-2000 mu L;
c. carrying out electrostatic spinning by adopting the electrostatic spinning solution prepared in the step b to obtain an electrostatic spinning film;
d. c, drying the electrostatic spinning membrane prepared in the step c in sequence to remove the solvent, and performing surface functionalization treatment on the dopamine to obtain a functionalized membrane; the dopamine surface functionalization treatment process comprises the following steps: soaking the electrostatic spinning membrane in the dopamine solution under the condition of oxygen supply, then taking out, washing with distilled water, and drying;
e. d, soaking the functional membrane prepared in the step d in a pre-prepared antibacterial polypeptide solution, and then taking out the functional membrane, wherein the concentration of the antibacterial polypeptide solution is 0.1-10 mg/mL;
f. and e, washing the film prepared in the step e with distilled water and drying.
2. The method for preparing a membrane for guiding bone tissue regeneration according to claim 1, wherein in step a, the medical degradable material is at least one of poly (lactic-co-glycolic acid), polycaprolactone, polylactic acid, and poly (beta-hydroxybutyrate).
3. The method for preparing a membrane for guiding bone tissue regeneration according to claim 1, wherein in step a, the organic solvent is at least one of trifluoroethanol, acetone, hexafluoroisopropanol, dimethylformamide and tetrahydrofuran.
4. The method for preparing a membrane for guiding bone tissue regeneration according to claim 1, wherein dexamethasone is dissolved in absolute ethanol to prepare a dexamethasone solution in step b.
5. The method for preparing a membrane for leading bone tissue regeneration according to claim 1, wherein in step c, electrospinning is performed in an electrospinning device under the following conditions: the voltage is 5-30 kV, the receiving distance is 5-30 cm, and the sample introduction speed is 0.5-5 mL/h.
6. The method for preparing a membrane for guiding bone tissue regeneration according to claim 1, wherein in step d, dopamine is added into a Tris-HCl solution to prepare a dopamine solution with a pH value of 5-11 and a concentration of 0.1-50 mg/mL.
7. The method for preparing a membrane for inducing bone tissue regeneration according to claim 1, wherein in step e, the antibacterial polypeptide in the antibacterial polypeptide solution is Inverso-CysHHC10, HHC-36, Tet213 or c-hLF 1-11.
8. A guided bone tissue regeneration membrane produced by the production method according to any one of claims 1 to 7.
9. Use of the guided bone tissue regeneration membrane of claim 8 in the preparation of a bone repair material.
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| CN111529682A (en) * | 2020-05-11 | 2020-08-14 | 中国人民解放军陆军军医大学第一附属医院 | Chemotaxis antibacterial nano material and preparation method and application thereof |
| CN112107728A (en) * | 2020-09-01 | 2020-12-22 | 广东工业大学 | Antibacterial peptide beta-HBD-3 loaded PCL/Zif-8 tissue engineering scaffold material and preparation method thereof |
| CN112402699A (en) * | 2020-12-09 | 2021-02-26 | 新疆师范大学 | Preparation method of polylactic acid/dexamethasone/nano-metal composite fiber membrane with osteoinduction and antibacterial functions |
| CN113198044A (en) * | 2021-04-08 | 2021-08-03 | 华南理工大学 | HHC 36-loaded polydopamine-functionalized hydroxyapatite composite material and preparation method and application thereof |
| CN113370533B (en) * | 2021-04-25 | 2022-12-06 | 中国人民解放军空军军医大学 | Preparation method of 3D printing shapeable guide bone regeneration membrane |
| CN114404672B (en) * | 2022-01-19 | 2023-03-03 | 四川大学 | Fiber membrane combining polyphenol and antibacterial peptide as well as preparation method and application of fiber membrane |
| CN117100621B (en) * | 2023-10-24 | 2024-01-09 | 山东一飞环保材料科技有限公司 | Antibacterial nanofiber dry mask and preparation method thereof |
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