CN109916974A - A kind of electrochemical detection method of quick measurement quinine content and its application - Google Patents

Abstract

The present invention relates to a kind of electrochemical detection method of quickly measurement quinine content and its applications.Include the following steps: using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl constitutes three-electrode system as reference electrode, after three-electrode system is placed in the electrolyte acetonitrile solution containing quinine, it is connect with electrochemical workstation, using differential pulse voltammetry, scanning voltage range: -0.5V~1.2V, scanning speed: 0.1V/s, amplitude: 50mV, pulse width: 200ms, measurement -0.2V peak position current value.Method of the invention can be directly used for the quinine content in measurement quinine beverage, and detection is limited to 8 × 10^‑6Mol/L is not necessarily to sample pretreatment, method is easy to operate and reaction condition is mildly controllable, and drug used is cheap and easy to get, and detection limit is low, favorable reproducibility using the quinine content in method measurement quinine beverage of the invention.

Description

A kind of electrochemical detection method of quick measurement quinine content and its application

Technical field

The present invention relates to technical field of electrochemical detection, and in particular to one kind is novel quickly to measure sample with bare glassy carbon electrode The electrochemical detection method of quinine content and its application in product.

Background technique

Quinine (peaceful -9- alcohol of 6'- methoxyl group golden pheasant) is a kind of natural white crystals alkaloid, and bitter has and brings down a fever, resists Malaria, analgesia and anti-inflammatory effect.The bark production of the Cinchona species of South America has many alkaloids, and quinine is wherein most heavy The alkaloid wanted.In history, quinine is used as anti-malaria medicaments more than 300 years.Quinine is mainly used for treatment to other antimalarials Resistant pernicious malaria.For treat malaria during, the cure rate of quinine is higher, at the same also show antibacterial, it is antipyretic, Local anaesthesia, cardiovascular excitant and analgesic properties and the excitability for reducing motor end plate.Other than medicinal, quinine is also used Make tonic, preservative and lotion.Quinine can also be used to prevent arrhythmia cordis, be the flavor components for nourishing water and bitter taste lemon.And Quinine is also a kind of potential drug toxicity, and some country's orders must indicate quinine concentration on food labelling.

Electrochemical method (electrochemical analysis) is that one kind builds the electrochemical properties of substance and solution The instrument analytical method of vertical connection.According to certain electrical parameter (such as resistance, conductance, current potential, electric current, electricity or electric current-electricity of battery Buckle line etc.) and the concentration of measured matter between the method that is measured there are certain relationship.Electrochemical Detection usually has Have the advantage that electrochemical detector is cheap compared with spectroscopic detector, electrochemical appliance (including electrode) can easily most Smallization is to develop the portable sensor with simple program, and energy requirement is low, can be used for laboratory or in-site measurement. Electrochemistry is to the analysis of the qualitative, quantitative of substance in food, drug, industrial production by extensive development and application.Electroactive agents Derivatization and modified electrode isoelectric analysis method allow sensitive and selectively measure quinine, but these method preparation process are multiple It is miscellaneous, poor reproducibility, and the preparation of electrode takes time and effort with maintenance.Therefore find simple and fast, detection limit is low, favorable reproducibility The method of Electrochemical Detection quinine content is very necessary.

Summary of the invention

In view of the problems of the existing technology, the purpose of the present invention is to provide a kind of simple and quick, detection limit is low, reappears The electrochemical detection method of quinine content in the good quick test sample of property.

To achieve the purpose of the present invention, The technical solution adopted by the invention is as follows: a kind of electricity of quickly measurement quinine content Chemical detection method includes the following steps: that using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl makees For reference electrode, three-electrode system is constituted, after three-electrode system is placed in the electrolyte acetonitrile solution containing quinine, with electrochemistry Work station connection measures the peak position current value of the electrolyte acetonitrile solution containing quinine using differential pulse voltammetry.

Preferably, the condition of the differential pulse voltammetry is scanning voltage range: -0.5V~1.2V, scanning speed: 0.1V/s, amplitude: 50mV, pulse width: 200ms.

It is furthermore preferred that the peak position current value is -0.2V peak position current value.

Preferably, above-mentioned one kind quickly measures the electrochemical detection method of quinine content, including is lied prostrate using differential pulse An Fa establishes the standard curve or linear equation of peak position current value Yu quinine concentration.

It is furthermore preferred that establishing the standard curve or linear of peak position current value and quinine concentration using differential pulse voltammetry Equation, the specific method is as follows: using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl is as reference electricity Pole constitutes three-electrode system, after three-electrode system is placed in the electrolyte acetonitrile solution containing various criterion quinine concentration, with Electrochemical workstation connection, using differential pulse voltammetry, measures the electrolyte acetonitrile containing various criterion quinine concentration respectively - 0.2V peak position the current value of solution, the standard curve or linear equation of foundation -0.2V peak position current value and quinine concentration；It is described The preparation method of electrolyte acetonitrile solution containing various criterion quinine concentration is: tetra-n-butyl six is sequentially added in volumetric flask Fluorophosphoric acid ammonium, hydroquinone and quinine standard solution, are settled to 10mL with acetonitrile, so that tetra-n-butyl ammonium hexafluorophosphate final concentration For 0.1mol/L, the final concentration of 1.5mmol/L of hydroquinone, standard quinine concentration range is 0~1.0mmol/L；The difference The condition of pulse voltammetry is scanning voltage range: -0.5V~1.2V, scanning speed: 0.1V/s, amplitude: 50mV, pulse are wide Degree: 200ms.

Preferably, when measuring quinine content in sample to be tested, the preparation side of the electrolyte acetonitrile solution containing quinine Method is: sequentially adding tetra-n-butyl ammonium hexafluorophosphate, hydroquinone and 0.5mL quinine sample to be measured in volumetric flask, uses acetonitrile It is settled to 10mL, so that the final concentration of 0.1mol/L of tetra-n-butyl ammonium hexafluorophosphate, the final concentration of 1.5mmol/L of hydroquinone.

Preferably, it is pre-processed including bare glassy carbon electrode: being respectively 1.0 and 0.3 μm of oxidation with granularity by bare glassy carbon electrode Aluminium powder looking-glass finish surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water.

Preferably, Electrochemical Detection carries out at room temperature.

Application of the above-mentioned electrochemical detection method in quickly measurement drug, health care product or food in quinine content.It is excellent Choosing, the food includes quinine beverage.

The present invention uses three electrode work systems, and the electrochemical oxidation based on hydroquinone in acetonitrile uses difference arteries and veins Voltammetry is rushed, quinine can cause two new oxidation peaks in position more negatively.The peak height of new oxidation peak depends on quinine concentration, establishes The linear relationship of peak height and concentration.New oxidation peak spike potential depends on two nitrogenous base pK of quinine_bDifference, can be used for identifying Other alkali.

The beneficial effects of the present invention are:

1, the present invention uses bare glassy carbon electrode, in aprotic solvent acetonitrile, using differential pulse method (DPV), anode It is positive, detects quinine content, sensitivity 6.01mA/mol, detection method is simple, and reaction condition is mildly controllable, drug valence used Honest and clean to be easy to get, detection limit is low, favorable reproducibility.

2, the present invention obtains proton using quinoline ring in quinine and contains in the height detection quinine of -0.2V new oxidation peak generated Amount, new peak are stablized, and influence factor is few, improve detection accuracy.

3, method of the invention can be directly used for the quinine content in measurement quinine beverage, and detection is limited to 8 × 10^-6Mol/L, Using the quinine content in method measurement quinine beverage of the invention, it is not necessarily to sample pretreatment, this method is easy to operate and reacts Mild condition is controllable, and drug used is cheap and easy to get, and detection limit is low, favorable reproducibility.

4, detection method of the invention, water content and hydroquinone content, on the measurement of quinine content without influence.

5, the present invention uses bare glassy carbon electrode for working electrode, good conductivity, and chemical stability is high, and coefficient of thermal expansion is small, matter Ground is hard, and air-tightness is good, and the potential scope of application is wide.

Detailed description of the invention

Fig. 1 is detection device three-electrode system schematic diagram of the present invention.

Fig. 2 is quinine and the hydroquinone cyclic voltammogram in acetonitrile；

Wherein, a:0.1mol/L TBAPF₆+1.5mmol/L QH₂；

b:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.4mmol/L QN；

c:0.1mol/L TBAPF₆+0.4mmol/L QN。

Fig. 3 is quinine and various concentration hydroquinone differential pulse figure in acetonitrile；

Wherein, a:0.1mol/L TBAPF₆+1.2mmol/L QH₂+0.6mmol/L QN

b:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN。

Fig. 4 is the differential pulse figure of quinine and different moisture content.

Fig. 5 is the differential pulse voltammetry figure of quinine and hydroquinone Fluid Dynamics sample and mark-on；

Wherein, a:0.1mol/L TBAPF₆+1.5mmol/L QH₂+ 0.4mmol/L QN+0.5mL water

b:0.1mol/L TBAPF₆+1.5mmol/L QH₂+ 0.4mmol/L QN+0.5mL chaff interferent.

Fig. 6 is standard quinine content detection and canonical plotting.

Specific embodiment

Embodiment 1.

The preparation of quinine (QN) standard solution: weighing 0.0130g quinine standard items, with the dissolution of 10ml acetonitrile and constant volume, system The quinine standard solution that standby normal concentration is 4mM, it is spare as mother liquor.

1, bare glassy carbon electrode pre-processes: bare glassy carbon electrode is polished with the alumina powder that granularity is respectively 1.0 and 0.3 μm It to specular surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water, it is spare.

2, the preparation of the electrolyte acetonitrile solution containing quinine: in 3 10mL volumetric flasks, it is separately added into tetra-n-butyl hexafluoro Ammonium phosphate (TBAPF₆), hydroquinone (QH₂) and quinine (QN) standard solution, it is settled to 10mL with acetonitrile, obtains supporting electrolyte Prepare liquid.

Supporting electrolyte 1:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.4mmol/L QN

Supporting electrolyte 2:0.1mol/L TBAPF₆+1.5mmol/L QH₂

Supporting electrolyte 3:0.1mol/L TBAPF₆+0.4mmol/L QN

3, as shown in Figure 1, using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl is as reference Electrode constitutes three-electrode system, after three-electrode system is placed in the prepare liquid of supporting electrolyte, connects with electrochemical workstation It connects, is scanned using cyclic voltammetry, scanning voltage range: -0.5V~1.2V, scanning speed: 0.1V/s.As a result such as Fig. 2.

It is scanned using cyclic voltammetry, scanning voltage range: -0.5V -1.2V, scanning speed: 0.1V/s, it can by Fig. 2 See, ((curve c) appears in 0.8 He in the oxidation peak Potential distribution of acetonitrile to curve a) to hydroquinone in Fig. 2 with quinine in Fig. 2 At 0.7V, curve b shows that the solution of the substance containing there are two types of generates two new peaks (in Fig. 2 in curve b in ± 0.2V or so in Fig. 2 II and III)；Because the new peak is the preferred electrochemistry work of the present invention caused by receiving the proton of hydroquinone release as quinine Making window is -0.5V~1.2V, obtains proton with quinoline ring in quinine and surveys in the peak position current value of -0.2V new oxidation peak generated Determine quinine content.

(1) influence of the hydroquinone content to quinine content measurement

1, bare glassy carbon electrode pre-processes: bare glassy carbon electrode is polished with the alumina powder that granularity is respectively 1.0 and 0.3 μm It to specular surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water, it is spare.

2, the preparation of the electrolyte acetonitrile solution containing quinine: in 2 10mL volumetric flasks, it is separately added into tetra-n-butyl hexafluoro Ammonium phosphate (TBAPF₆), hydroquinone (QH₂) and quinine (QN) standard solution, it is settled to 10mL with acetonitrile, obtains supporting electrolyte Prepare liquid.

Supporting electrolyte 1:0.1mol/L TBAPF₆+1.2mmol/L QH₂+0.6mmol/L QN

Supporting electrolyte 2:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN

3, as shown in Figure 1, using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl is as reference Electrode constitutes three-electrode system, after three-electrode system is placed in the prepare liquid of supporting electrolyte, connects with electrochemical workstation It connects, is scanned using differential pulse voltammetry, scanning voltage range: -0.5V~1.2V, scanning speed: 0.1V/s, amplitude: 50mV, Pulse width: 200ms, measurement -0.2V peak position current value.As a result such as Fig. 3.

As seen from Figure 3, with the increase of hydroquinone concentration, the peak current at peak I increases, and peak II and peak III are in -0.2V Peak position current value is kept constant, if using the concentration of the peak current at peak II and peak III measurement quinine, the concentration pair of hydroquinone The measurement of quinine content does not influence.

In Fig. 3, curve a is 1.2mmol/L QH₂, curve b is 1.5mmol/L QH₂, with QH₂The variation diagram 3 of concentration In ± 0.2V peak position current value (peak II and peak III in Fig. 3) do not influence, it is known that the content of hydroquinone does not have quinine measurement It influences.

(2) influence of the water content to quinine content measurement

1, bare glassy carbon electrode pre-processes: bare glassy carbon electrode is polished with the alumina powder that granularity is respectively 1.0 and 0.3 μm It to specular surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water, it is spare.

2, the preparation of the electrolyte acetonitrile solution containing quinine: in 7 10mL volumetric flasks, it is separately added into tetra-n-butyl hexafluoro Ammonium phosphate, hydroquinone, quinine standard solution and water (0,0.05,0.10,0.15,0.20,0.30,0.50mL), it is fixed with acetonitrile Hold to 10mL, obtains the prepare liquid of supporting electrolyte.

Supporting electrolyte 1:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN+0mL H₂O

Supporting electrolyte 2:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN+0.05mL H₂O

Supporting electrolyte 3:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN+0.1mL H₂O

Supporting electrolyte 4:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN+0.15mL H₂O

Supporting electrolyte 5:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN+0.2mL H₂O

Supporting electrolyte 6:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN+0.3mL H₂O

Supporting electrolyte 7:0.1mol/L TBAPF₆+1.5mmol/L QH₂+0.6mmol/L QN+0.5mL H₂O

3, as shown in Figure 1, using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl is as reference Electrode constitutes three-electrode system, after three-electrode system is placed in the prepare liquid of supporting electrolyte, connects with electrochemical workstation It connects, is scanned using differential pulse voltammetry, scanning voltage range: -0.5V~1.2V, scanning speed: 0.1V/s, amplitude: 50mV, Pulse width: 200ms, measurement -0.2V peak position current value.As a result such as Fig. 4.

From fig. 4, it can be seen that peak I is gradually mobile to peak II, and the peak current at peak II is gradually with the increase of water concentration in electrolyte Increase, and the peak current at peak III is unaffected when the water volume of addition is less than 0.3mL, therefore is measured using the peak current at peak III When quinine content, the water of small concentration does not influence the measurement of quinine.

In Fig. 4, the water of addition is represented successively from right to left as 0~0.5mL.With the increase of dampening additional amount, in Fig. 4- 0.2V peak position current value (peak III in Fig. 4) has little effect, it is known that the presence of water does not influence quinine measurement.

(3) influence of the chaff interferent to quinine content measurement

1, bare glassy carbon electrode pre-processes: bare glassy carbon electrode is polished with the alumina powder that granularity is respectively 1.0 and 0.3 μm It to specular surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water, it is spare.

2, the preparation of the electrolyte acetonitrile solution containing quinine: in 2 10mL volumetric flasks, tetra-n-butyl hexafluoro is sequentially added Ammonium phosphate, hydroquinone, quinine standard solution and 0.5mL water or 0.5mL chaff interferent mixed solution (0.1mM sucrose, 0.05mM Ah This Ba Tian, 0.1mM acesulfame potassium, 0.1mM glycine), it is settled to 10mL with acetonitrile, obtains the prepare liquid of supporting electrolyte.

Supporting electrolyte 1:0.1mol/L TBAPF₆+1.5mmol/L QH₂+ 0.4mmol/L QN+0.5mL water

Supporting electrolyte 2:0.1mol/L TBAPF₆+1.5mmol/L QH₂The mixing of+0.4mmol/L QN+0.5mL chaff interferent Solution

3, as shown in Figure 1, using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl is as reference Electrode constitutes three-electrode system and connect after three-electrode system to be placed in the prepare liquid of supporting electrolyte with electrochemical workstation, Using differential pulse voltammetry, scanning voltage range: -0.5V~1.2V, scanning speed are as follows: 100mv/s, amplitude: 50mV, pulse Width: 200ms, measurement -0.2V peak position current value.As a result as shown in Figure 5.

As seen from Figure 5, curve a is that 0.5mL water is added in Fig. 5, and curve b is that 0.5mL chaff interferent mixed solution is added, and is added The peak current of chaff interferent postpeak III keeps stablizing, it was demonstrated that above-mentioned chaff interferent does not influence the measurement of quinine.The presence of chaff interferent Quinine measurement is not influenced.

Embodiment 2.

Electrochemical detection method quickly measures quinine content in quinine beverage

(1) standard curve and linear equation of foundation -0.2V peak position current value and quinine concentration

1, bare glassy carbon electrode pre-processes: bare glassy carbon electrode is polished with the alumina powder that granularity is respectively 1.0 and 0.3 μm It to specular surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water, it is spare.

2, it the preparation of the electrolyte acetonitrile solution containing various criterion quinine concentration: in 10mL volumetric flask, sequentially adds Tetra-n-butyl ammonium hexafluorophosphate, hydroquinone and quinine standard solution, are settled to 10mL with acetonitrile, respectively obtain tetra-n-butyl six The final concentration of 0.1mol/L of fluorophosphoric acid ammonium, the final concentration of 1.5mmol/L of hydroquinone, quinine standard final concentration is respectively 0,0.2, 0.4, the electrolyte acetonitrile solution of 0.6,0.8,1.0mmol/L.

It compares simultaneously, contrast groups, which become, is added tetra-n-butyl ammonium hexafluorophosphate and quinine standard solution, with acetonitrile constant volume To 10mL, the final concentration of 0.1mol/L of tetra-n-butyl ammonium hexafluorophosphate is obtained, the final concentration of 0.4mmol/L of quinine standard is free of There is the electrolyte acetonitrile solution of hydroquinone.

3, as shown in Figure 1, using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl is as reference Electrode constitutes three-electrode system, after three-electrode system is placed in the electrolyte acetonitrile solution containing quinine, with electrochemical operation It stands connection, using differential pulse voltammetry, scanning voltage range: -0.5V~1.2V, scanning speed are as follows: 100mv/s, amplitude: 50mV, pulse width: 200ms, measurement -0.2V peak position current value.As a result as shown in Figure 6.

As seen from Figure 6, for quinine concentration in the range of 0.2~1.0mmol/L, the peak current at peak III is with quinine concentration Increase and be continuously increased, by do figure it can be concluded that the variable quantity of its concentration and -0.2V peak position current value there are linear relationship, lines Property equation are as follows: y=6.011x-0.0587, in formula: x is the concentration (mmol/L) of quinine；Y is -0.2V peak position current value (μ A).

(2) in quinine beverage quinine content measurement

1, bare glassy carbon electrode pre-processes: bare glassy carbon electrode is polished with the alumina powder that granularity is respectively 1.0 and 0.3 μm It to specular surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water, it is spare.

2, the preparation of the electrolyte acetonitrile solution containing quinine: in 10mL volumetric flask, tetra-n-butyl hexafluoro phosphorus is sequentially added Sour ammonium, hydroquinone and 0.5mL quinine beverage, are settled to 10mL with acetonitrile, it is final concentration of to obtain tetra-n-butyl ammonium hexafluorophosphate The electrolyte acetonitrile solution containing quinine of the final concentration of 1.5mmol/L of 0.1mol/L, hydroquinone.

3, as shown in Figure 1, using bare glassy carbon electrode as working electrode, platinum electrode is used as to electrode, and Ag/AgCl is as reference Electrode constitutes three-electrode system, after three-electrode system is placed in the electrolyte acetonitrile solution containing quinine, with electrochemical workstation Connection, using differential pulse voltammetry, scanning voltage range: -0.5V~1.2V, scanning speed are as follows: 100mv/s, amplitude: 50mV, pulse width: 200ms, measurement -0.2V peak position current value.

According to linear equation: y=6.011x-0.0587, in formula: x is the concentration (mmol/L) of quinine；Y is -0.2V peak position Current density value (μ A), is calculated quinine concentration in sample liquid, and then obtain the content of quinine in quinine beverage.

(3) result

Commercially available quinine beverage is detected, has detected 3 batches, the color with the detection quinine content of maturation in the prior art Spectral method is compared, and accuracy rate reaches 100%.Up to 6.01mA/mol, detection limit is low to can reach 8 × 10 for sensitivity of the invention^{- 6}mol/L。

Claims (10)

1. a kind of electrochemical detection method of quickly measurement quinine content, which comprises the steps of: with naked glass carbon electricity Pole is as working electrode, and platinum electrode is used as to electrode, and Ag/AgCl constitutes three-electrode system, by three electrode bodies as reference electrode After system is placed in the electrolyte acetonitrile solution containing quinine, it is connect with electrochemical workstation, using differential pulse voltammetry, measurement contains The peak position current value of the electrolyte acetonitrile solution of quinine.

2. a kind of electrochemical detection method of quickly measurement quinine content according to claim 1, which is characterized in that described The condition of differential pulse voltammetry is scanning voltage range: -0.5V~1.2V, scanning speed: 0.1V/s, amplitude: 50mV, arteries and veins Rush width: 200ms.

3. a kind of electrochemical detection method of quickly measurement quinine content according to claim 2, which is characterized in that described Peak position current value is -0.2V peak position current value.

4. a kind of electrochemical detection method of quickly measurement quinine content according to claim 1, which is characterized in that including Using differential pulse voltammetry, the standard curve or linear equation of peak position current value Yu quinine concentration are established.

5. a kind of electrochemical detection method of quickly measurement quinine content according to claim 4, which is characterized in that use Differential pulse voltammetry establishes the standard curve or linear equation of peak position current value Yu quinine concentration, the method is as follows: with naked glass carbon Electrode is as working electrode, and platinum electrode is used as to electrode, and Ag/AgCl constitutes three-electrode system, by three electrodes as reference electrode After system is placed in the electrolyte acetonitrile solution containing various criterion quinine concentration, it is connect with electrochemical workstation, using difference Pulse voltammetry measures the -0.2V peak position current value of the electrolyte acetonitrile solution containing various criterion quinine concentration respectively, builds The standard curve or linear equation of vertical -0.2V peak position current value and quinine concentration；The electricity containing various criterion quinine concentration The preparation method of solution matter acetonitrile solution is: tetra-n-butyl ammonium hexafluorophosphate, hydroquinone and quinine are sequentially added in volumetric flask Standard solution is settled to 10mL with acetonitrile, so that tetra-n-butyl hexafluorophosphoric acid ammonium concentration is 0.1mol/L, hydroquinone concentration is 1.5mmol/L, standard quinine concentration range are 0~1.0mmol/L；The condition of the differential pulse voltammetry is scanning voltage Range: -0.5V~1.2V, scanning speed: 0.1V/s, amplitude: 50mV, pulse width: 200ms.

6. a kind of electrochemical detection method of quickly measurement quinine content according to claim 5, which is characterized in that measurement In sample to be tested when quinine content, the preparation method of the electrolyte acetonitrile solution containing quinine is: successively adding in volumetric flask Enter tetra-n-butyl ammonium hexafluorophosphate, hydroquinone and 0.5mL quinine sample to be measured, be settled to 10mL with acetonitrile, so that four positive fourths Base hexafluorophosphoric acid ammonium concentration is 0.1mol/L, and hydroquinone concentration is 1.5mmol/L.

7. the electrochemical detection method of described in any item a kind of quickly measurement quinine contents according to claim 1~6, feature It is, is pre-processed including bare glassy carbon electrode: bare glassy carbon electrode is polished with the alumina powder that granularity is respectively 1.0 and 0.3 μm It to specular surface, is rinsed with secondary water, is then ultrasonically treated 30 seconds in pure water.

8. the electrochemical detection method of described in any item a kind of quickly measurement quinine contents according to claim 1~6, feature It is, Electrochemical Detection carries out at room temperature.

9. the described in any item electrochemical detection methods of claim 1~6 quinine in quickly measurement drug, health care product or food Application in content.

10. application according to claim 9, which is characterized in that the food includes quinine beverage.