CN109845940A - A kind of preparation method of function chestnut drink - Google Patents
A kind of preparation method of function chestnut drink Download PDFInfo
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Abstract
The present invention relates to a kind of preparation methods of function chestnut drink, are related to the preparation for processing technical field of food and drink, comprising the following steps: remove the peel the Chinese chestnut after cleaning to obtain Chinese chestnut Seed, homogeneous, calcium ion, deodorization and sterilization processing is added in Chinese chestnut Seed crushing；Slashings chestnut is adjusted to high temperature, and calcium chloride is added, and Thermostable α-Amylase is added and sufficiently digests to feed liquid；It adds carbohydrase and feed liquid is sufficiently digested；Feed liquid is adjusted to protease optimal reaction temperature and pH, after add protease feed liquid sufficiently digested；Product after protein digestion is subjected to destroy the enzyme treatment, after destroy the enzyme treatment carries out that product is obtained by filtration.The chestnut starch comparision contents that the present invention uses are high, using Thermostable α-Amylase and Glucoamylase hydrolysis chestnut starch, degree of hydrolysis is very high, will not hinder the hydrolysis of protein, the protein in the efficient hydrolysis Chinese chestnut of protease can be used, and small peptide content of the amino acid number in 2-10 is higher.
Description
Technical field
The present invention relates to the preparation for processing technical field of food and drink, it is specifically related to a kind of function chestnut drink
Preparation method.
Background technique
Unsaturated fatty acid and vitamin abundant, minerals contained in Chinese chestnut, can prevent and treat high blood pressure, coronary heart disease,
Artery sclerosis, sclerotin dredge etc. diseases, be anti-aging, the excellent tonic product promoted longevity, be known as the laudatory title of " king of dry fruit ", with jujube,
Persimmon is simultaneously known as " iron crops ", " woody grain ".Wherein, Chinese chestnut is the higher dry fruit kind of carbohydrate content, can be supplied
It gives human body more thermal energy, and fat metabolism can be helped, have and replenish qi to invigorate the spleen, the effect of Mangnolia officinalis stomach and intestine.Chinese chestnut contains riboflavin,
It often eats beneficial to the passing of time refractory children's aphthae and adult canker sore；Chinese chestnut vitamin C rich in, is able to maintain that
The normal function of tooth, bone, vascular muscle, can prevent and treat osteoporosis, lassitude in loin and legs, arthralgia and myalgia, out of strength etc.,
Delaying human body caducity is the ideal health fruit of the elderly.
The protein of starch and nearly 8% of the Chinese chestnut containing 40%-60%.The protein of macromolecular is often not easy to be absorbed completely,
The absorption rate of Chinese chestnut nutrition is smaller.Simultaneously for the sufferer of gastricism, edible protein adsorption is restricted, body
It is inside unable to synthesis polypeptide, the circulation of protein and amino acids is caused to be destroyed, selection function peptide oral liquid is needed to supplement polypeptide.It is small
Molecular peptide has the characteristics that preferentially to be digested, and very good for cardiovascular disease, can remove blood vessel lactones, adjusts
Cardiac function is saved, cardiovascular function is restored.
Summary of the invention
Efficient micromolecule polypeptide can be resolved into solve protein contained in Chinese chestnut, prepare functional richness
The technical issues of chestnut drink containing small peptide, the present invention provides a kind of preparation method of function chestnut drink.
In order to achieve the object of the present invention, the invention adopts the following technical scheme: a kind of preparation of function chestnut drink
Method, comprising the following steps:
The pre-treatment of S1, Chinese chestnut: it removes the peel the Chinese chestnut after over cleaning to obtain Chinese chestnut Seed, after Chinese chestnut Seed is crushed again
Carry out homogeneous；Calcium ion is added after the completion of homogeneous, and carries out deodorization and sterilization processing under high temperature；
S2, amylorrhexis: being adjusted to high temperature with the slashings chestnut after sterilization processing to deodorization and maintain temperature constant, and chlorination is added
Calcium stirs 8-10min, and the rear Thermostable α-Amylase that is added sufficiently is digested to starch；Add again after the completion of Thermostable α-Amylase enzymatic hydrolysis
Enter carbohydrase and is sufficiently digested to feed liquid；
S3, protein digestion: the feed liquid after carbohydrase is sufficiently digested is adjusted to protease optimum enzymolysis condition, after add
Protease sufficiently digests feed liquid；
S4, enzyme deactivation: the product after protein digestion is subjected to destroy the enzyme treatment, is rich in after destroy the enzyme treatment through being obtained by filtration
The chestnut drink of small peptide.
Preferably, homogeneous described in S1 carries out in homogeneous tank；The specific steps of the homogeneous are as follows: by smashed plate
Chestnut is transferred in homogeneous tank, and keeping pressure inside the tank constant is 30Mpa, stirs 15min~20min；Deodorization and sterilization processing described in S1
Condition are as follows: at 140 DEG C, constant temperature handle 5~6s.
Preferably, S2 amylase enzymolysis process carries out in Biochemical pot；The specific steps of S2 amylorrhexis are as follows: warp
It in slashings Li Fangzhi Biochemical pot after crossing deodorization and sterilization, adjusts in tank to 96 DEG C of constant temperature, 0.02wt% is added by Chinese chestnut quality
0.01mol/L calcium chloride solution stirring 10min after；PH to 6.5 is adjusted, then 0.08wt% enzyme activity is added by Chinese chestnut quality and is
The Thermostable α-Amylase of 4000U/g digests 70min；Temperature is to 65 DEG C of constant temperature in adjusting tank, after adjusting pH to 5.0, then pressing board
Li Zhiliang is added the carbohydrase that 1.5wt% enzyme activity is 100,000 U/g and digests 120min.
Preferably, protease described in S3 protein digestion is alkali protease or neutral proteinase.
Preferably, the specific steps of S3 protein digestion are as follows: adjust the temperature to 56 DEG C of constant temperature, pH is constant to 7.0, sugared
The enzyme activity that 3200U/g albumen is added in enzymolysis liquid after changing enzyme enzymatic hydrolysis is the neutral proteinase of 200,000 U/g；Enzymolysis time is
4.5h；
Or the specific steps of S3 protein digestion are as follows: adjust the temperature to 55 DEG C of constant temperature, pH is constant to 8.5, glucoamylase enzyme
The enzyme activity that 3000U/g albumen is added in enzymolysis liquid after solution is the alkali protease of 200,000 U/g；Enzymolysis time is 4h.
Preferably, the reaction condition of destroy the enzyme treatment described in S4 enzyme deactivation is to react 5~10min at 120 DEG C；The filtering
For diatomite plate-frame filtering.
Preferably, S4 enzyme deactivation after also by concentration with it is filling；The concentration carries out in concentration tank；
The concentration and filling specific steps are as follows: the chestnut drink after concentration through being obtained by filtration, then through filling obtained tank
Chestnut drink is filled, canned chestnut drink high-temp steam sterilizing obtains finished product.
Preferably, altogether by three concentration tank processing before and after the concentration；After the concentration by
It is filtered into 0.1-0.3 μm of fine ga(u)ge screen filtering.
Preferably, cleaning described in the pre-treatment of S1 Chinese chestnut is that clear water cleans three times repeatedly；Described crush is three glue
Body mill is ground, and vibrating screen is equipped between adjacent twice colloid mill.
Preferably, carry out homogeneous after crushing described in the pre-treatment of S1 Chinese chestnut, smashed Chinese chestnut quickly into
Enter in homogeneous tank, the contact in order to avoid smashed Chinese chestnut with oxygen, the phenolic compound in slashings chestnut and oxygen occur
It is sent before enzymatic browning to homogeneous tank.
The beneficial effects of the present invention are: 1, the present invention be prepared small peptide content higher Chinese chestnut drinks, blood
Small peptide in circulation can directly participate in the synthesis of tissue protein, and furthermore liver, kidney, skin and its hetero-organization also can be completely
Utilize small peptide.Promote being absorbed and utilized for minerals, mainly with complex form in conjunction with minerals, increases mineral absorption.It is small
Peptide can compete to avoid the absorption between amino acid.Small peptide has physiological regulatory action.It can pass through enzymes some in inducing intestinal
Active raising, and shift to an earlier date the development of small intestine digestive function, promote health and improve its production performance, promote disappearing for nutriment
Change and absorb, small-molecular peptides have the characteristics that preferentially to be digested.The nutriment that people eat, in absorption, with
In the competition of active peptide, active peptide has the characteristics that preferential absorption.Small-molecular peptides have amino acid and protect when being absorbed by the body
The mixture of protective function, small peptide and amino acid is the optimal absorption mechanism of absorption of human body protein, and obtained beverage has promotion to absorb,
Immunity function is improved, physiological function, oxidation resistant effect are adjusted.
2, neutral proteinase pH in Chinese chestnut protein hydrolytic process is 7.0；When alkali protease is digested, pH control
System is 8.5.Since during enzymolysis protein matter, as the peptide bond of protein is constantly opened, free carboxyl out constantly increases
Add, needs plus alkali stablizes pH, protease can be kept efficiently to be digested always.Using neutral proteinase high starch feelings
The protein small molecule hydrolyzed in Chinese chestnut under condition obtains that peptide rate is relatively high, and especially amino acid forms the small peptide between 2~10, fits
Close the hydrolyzed state of this process.
3, the phenolic compound (PPO) and peroxidase (POD) original contained in Chinese chestnut is obstructed by cell, and cell is worked as
Because crushing or placing for a long time, phenolic compound and enzyme will contact with each other, while because of the participation of oxygen, phenolic compound because
It aoxidizes and generates melanin substance.Phenolic substances is oxidized under polyphenol oxidase (PPO) and peroxidase (POD) effect
Anthraquinone.The activity of Chinese chestnut Seed polyphenol oxidase is suppressed completely under high temperature, it is suppressed that the generation of melanin substance.But add
In the process of heat, Maillard reaction can occur again for Chinese chestnut leads to non-enzymatic browning, and calcium ion is added during processing Chinese chestnut,
The non-enzymatic browning that Chinese chestnut can be inhibited to occur at high operating temperatures.
4, content of starch is relatively high in Chinese chestnut, and amylose is easy to aging, and sticky sense is strong, poor taste.The present invention is stringent
Controlled enzymatic hydrolysis condition digests chestnut starch using Thermostable α-Amylase and carbohydrase, and degree of hydrolysis is up to 90%, significantly subtracts
The content of few amylose, also significantly reduces the obstruction that starch etc. hydrolyzes protein later, it can high using protease
Protein in the hydrolysis Chinese chestnut of efficiency.Amylase is additionally added calcium chloride before being added, and calcium chloride plays its flocculation, mentions
Taka-diastase heat resistance.
5, amylorrhexis uses dual-enzymatic process, and since the specificity of enzyme is strong, by-product is few, and the high enzymatic hydrolysis reaction of yield is slow
With, equipment requirement is low, saccharification concentration it is higher than sour water solution, it is possible to reduce steam concentration amount.This technical process is simple, can continuously into
Row, the substance that product is harmful to the human body without containing any hormone etc., obtains pure nutriment.
Specific embodiment
Following specific embodiments are described in further detail technical solution of the present invention.
Embodiment 1
S1, Jinzhai County Chinese chestnut is poured into clear water and is cleaned with hand, washed away its epidermis dirt to epidermis foreign, clean three repeatedly
It is secondary.Chinese chestnut after cleaning is gently poured into vibrating screen, Chinese chestnut is reached colloid mill, chestnut peel and Chinese chestnut Seed point by vibrating screen
From, by vibrating screen pass through three colloid mills, Chinese chestnut Seed can be thoroughly crushed.Smashed Chinese chestnut is passed through homogeneous tank, is kept
Atmospheric pressure in homogeneous tank maintains 20min at 30Mpa.Suitable calcium ion is added in homogeneous tank, is passed through hot steam, rises
High-temperature reaches 140 DEG C, at this temperature lasting 6s.
S2, the Chinese chestnut after deodorization, sterilization is passed through in Biochemical pot, the temperature in Biochemical pot is down to by pipe heat exchanger
96 DEG C, the 0.01mol/L calcium chloride that addition accounts for substrate Chinese chestnut 0.02wt% under constant temperature adjusts feed liquid after 10min is handled
PH is 6.5, then accounts for the Thermostable α-Amylase that substrate 0.08wt% enzyme activity is 4000U/g by the addition of Chinese chestnut quality and sufficiently digest
70min.Then 65 DEG C, then by pipe heat exchanger the temperature in Biochemical pot are down to, the feed liquid acid adding to have liquefied is adjusted PH
It is 5.0, the carbohydrase for accounting for substrate 1.5wt% is added by Chinese chestnut quality, 100,000 U/g of enzyme activity digests 120min with this condition.
S3, pipe heat exchanger cooling is continued through as 56 DEG C, the neutrality of addition 3200U/g albumen in feed liquid be saccharified
Protease, 200,000 U/g of enzyme activity, in enzymolysis process constantly plus alkali to maintain feed liquid PH be 7.0, hydrolyze 4.5h with this condition.
S4, the Chinese chestnut peptide solution digested in Biochemical pot is passed through hot steam, increases temperature to 120 DEG C, maintains 10min.?
Fire resistant alpha-diastase, carbohydrase, neutral proteinase all inactivate at a temperature of this.Enzymolysis liquid is flowed through slowly diatomite sheet frame,
Collect filtered solution.
S5, filtered solution is passed through to concentration tank, the concentration of Chinese chestnut peptide is successively promoted by three concentration tanks, continued after concentration
Liquid pass through 0.3 μm of fine ga(u)ge screen, reach more careful filtering, collect filtrate；Concentration, filtered Chinese chestnut peptide solution
Packing processes are successively carried out, hot steam is passed through after packaging, temperature reaches 115 DEG C, at this temperature, kills outside after Chinese chestnut peptide packaging
The pollutant in portion.
Embodiment 2
S1, Jinzhai County Chinese chestnut is poured into clear water and is cleaned with hand, washed away its epidermis dirt to epidermis foreign, clean three repeatedly
It is secondary.Chinese chestnut after cleaning is gently poured into vibrating screen, Chinese chestnut is reached colloid mill, chestnut peel and Chinese chestnut Seed point by vibrating screen
From, by vibrating screen pass through three colloid mills, Chinese chestnut Seed can be thoroughly crushed.Smashed Chinese chestnut is passed through homogeneous tank, is kept
Atmospheric pressure in homogeneous tank maintains 15min at 30Mpa.Suitable calcium ion is added in homogeneous tank, is passed through hot steam, rises
High-temperature reaches 140 DEG C, at this temperature lasting 5s.
S2, the Chinese chestnut after deodorization, sterilization is passed through in Biochemical pot, the temperature in Biochemical pot is down to by pipe heat exchanger
96 DEG C, the 0.01mol/L calcium chloride for accounting for substrate 0.02wt% is added under constant temperature, after 10min is handled, the PH for adjusting feed liquid is
6.5, then account for the Thermostable α-Amylase that substrate 0.08wt% enzyme activity is 4000U/g by the addition of Chinese chestnut quality and sufficiently digest 70min.
Then 65 DEG C, then by pipe heat exchanger the temperature in Biochemical pot are down to, it is 5.0 that the feed liquid acid adding to have liquefied, which is adjusted PH,
The carbohydrase for accounting for substrate 1.5wt% is added by Chinese chestnut quality, 100,000 U/g of enzyme activity digests 120min with this condition.
S3, pipe heat exchanger cooling is continued through as 55 DEG C, the alkalinity of addition 3000U/g albumen in feed liquid be saccharified
Protease, 200,000 U/g of enzyme activity, in enzymolysis process constantly plus alkali to maintain feed liquid PH be 8.5, hydrolyze 4h with this condition.
S4, the Chinese chestnut peptide solution digested in Biochemical pot is passed through hot steam, increases temperature to 120 DEG C, maintains 10min,
Fire resistant alpha-diastase, carbohydrase, neutral proteinase all inactivate at a temperature of this.Enzymolysis liquid is flowed through slowly diatomite sheet frame,
Collect filtered solution
S5, filtered solution is passed through to concentration tank, the concentration of Chinese chestnut peptide is successively promoted by three concentration tanks, continued after concentration
Liquid pass through 0.1 μm of fine ga(u)ge screen, reach more careful filtering, collect filtrate；Concentration, filtered Chinese chestnut peptide solution
Packing processes are successively carried out, hot steam is passed through after packaging, temperature reaches 115 DEG C, at this temperature, kills outside after Chinese chestnut peptide packaging
The pollutant in portion.
Comparative example 1
Comparative example 1 the difference from embodiment 1 is that: the Chinese chestnut after deodorization, sterilization is passed through biochemistry by S2, Starch Hydrolysis stage
In tank, the temperature in Biochemical pot is down to 96 DEG C by pipe heat exchanger, is added under constant temperature and accounts for substrate 0.02wt%'s
0.01mol/L calcium chloride, after 10min is handled, the PH for adjusting feed liquid is 6.5, then 0.08wt% enzyme is added by Chinese chestnut quality
Living is that the Thermostable α-Amylase of 4000U/g sufficiently digests 70min.Continuing through pipe heat exchanger cooling is 56 DEG C, is being liquefied
It is added the neutral proteinase of 3200U/g albumen in good feed liquid, 200,000 U/g of enzyme activity, in enzymolysis process constantly plus alkali maintains feed liquid
PH is 7.0, hydrolyzes 4.5h with this condition.This process only has Thermostable α-Amylase enzymatic hydrolysis, and without the enzymatic hydrolysis of carbohydrase.
Comparative example 2
Comparative example 2 the difference from embodiment 1 is that: the Chinese chestnut after deodorization, sterilization is passed through biochemistry by S2, Starch Hydrolysis stage
In tank, the temperature in Biochemical pot is down to 65 DEG C by pipe heat exchanger, it is 5.0 that acid adding, which adjusts PH, in feed liquid, by Chinese chestnut quality
The carbohydrase of 1.5wt% is added, 100,000 U/g of enzyme activity digests 120min with this condition.Continuing through pipe heat exchanger cooling is
56 DEG C, be added the neutral proteinase of 3200U/g albumen in the feed liquid being saccharified, 200,000 U/g of enzyme activity, in enzymolysis process constantly plus
It is 7.0 that alkali, which maintains feed liquid PH, hydrolyzes 4.5h with this condition.This process only has carbohydrase enzymatic hydrolysis, and without high temperature resistant α-shallow lake
The enzymatic hydrolysis of powder enzyme.
Comparative example 3
Comparative example 3 the difference from embodiment 1 is that: the Chinese chestnut after deodorization, sterilization is passed through biochemistry by S2, Starch Hydrolysis stage
In tank, it is 56 DEG C by pipe heat exchanger cooling, the neutral proteinase of 3200U/g albumen, 200,000 U/ of enzyme activity is added in feed liquid
G, in enzymolysis process constantly plus alkali to maintain feed liquid PH be 7.0, hydrolyze 4.5h with this condition.This process, which has no, is added high temperature resistant α-
Amylase enzymolysis, also without the enzymatic hydrolysis of carbohydrase.
Comparative example 4
S1, Jinzhai County Chinese chestnut is poured into clear water and is cleaned with hand, washed away its epidermis dirt to epidermis foreign, clean three repeatedly
It is secondary.Chinese chestnut after cleaning is gently poured into vibrating screen, Chinese chestnut is reached colloid mill, chestnut peel and Chinese chestnut Seed point by vibrating screen
From, by vibrating screen pass through three colloid mills, Chinese chestnut Seed can be thoroughly crushed.Smashed Chinese chestnut is passed through homogeneous tank, is kept
Atmospheric pressure in homogeneous tank maintains 20min at 30Mpa.Suitable calcium ion is added in homogeneous tank, is passed through hot steam, rises
High-temperature reaches 140 DEG C, at this temperature lasting 5s.
S2, the Chinese chestnut after deodorization, sterilization is passed through in Biochemical pot, the temperature in Biochemical pot is down to by pipe heat exchanger
96 DEG C, the 0.01mol/L calcium chloride that addition accounts for substrate Chinese chestnut 0.02wt% under constant temperature adjusts feed liquid after 10min is handled
PH is 6.5, then the Thermostable α-Amylase that 0.08wt% enzyme activity is 4000U/g is added by Chinese chestnut quality and sufficiently digests 70min.It connects
, then the temperature in Biochemical pot is down to 65 DEG C by pipe heat exchanger, it is 5.0 that the feed liquid acid adding to have liquefied, which is adjusted PH, is pressed
The carbohydrase of 1.5wt% is added in Chinese chestnut quality, and 100,000 U/g of enzyme activity digests 120min with this condition.
S3, pipe heat exchanger cooling is continued through as 55 DEG C, the pawpaw of addition 2500U/g albumen in feed liquid be saccharified
Protease, 200,000 U/g of enzyme activity, in enzymolysis process constantly plus alkali to maintain feed liquid PH be 7.0, hydrolyze 4h with this condition.
S4, the Chinese chestnut peptide solution digested in Biochemical pot is passed through hot steam, raising temperature is resistance at this temperature to 120 DEG C
Alpha-amylase, carbohydrase, papain all inactivate.Enzymolysis liquid is flowed through diatomite sheet frame slowly, collects filtered solution.
S5, filtered solution is passed through to concentration tank, the concentration of Chinese chestnut peptide is successively promoted by three concentration tanks, continued after concentration
Liquid pass through 0.2 μm of fine ga(u)ge screen, reach more careful filtering, collect filtrate；Concentration, filtered Chinese chestnut peptide solution
Packing processes are successively carried out, hot steam is passed through after packaging, temperature reaches 115 DEG C, at this temperature, kills outside after Chinese chestnut peptide packaging
The pollutant in portion.
Performance test: the Chinese chestnut peptide beverage that embodiment 1-2 and comparative example 1-4 are prepared carries out Starch Hydrolysis degree, egg
The measurement of white matter degree of hydrolysis, content of peptides and small peptide content (amino acid forms number in 2-10).
The measurement of Starch Hydrolysis degree: using Fehlings reagent, and degree of hydrolysis (DE) is the content X of reduced sugar in Chinese chestnut hydrolyzate
(g/100mL) with Chinese chestnut slurries in dry matter content A (g/100mL) ratio, i.e. DE (%)=X/A × 100%, indicate form sediment
The hydrolysis degree of powder.
The measurement of protein degree: the degree of hydrolysis (DH) of albumen is calculated using pH-state method, to reaction system
PH value carries out On-line Control.
Formula:
In formula: B is the volume of NaOH, mL；MbFor the concentration of NaOH, mol/L；MpFor the quality of substrate protein white matter, g；htot
For mM number of peptide bond in every gram of material protein.The degree of dissociation a of amino is calculated as follows: l/a=1+10pK-pH.
The measurement of content of peptides: ultraviolet spectrophotometry, using reduced form GSH (glutathione) concentration as abscissa, extinction
Angle value is that ordinate draws standard curve, and gradient concentration is respectively as follows: the GSH (paddy of 0.05,0.1,0.2,0.3,0.4,0.5mg/mL
The sweet peptide of Guang) solution, its absorbance value is measured under 238nm wavelength.Sample measurement is carried out after handling sample, takes 1mL sample solution,
It uses 1mL distilled water as control, the content of peptides in sample can be calculated according to standard curve.
Gel chromatography chromatography measures peptide molecular weight range and its content: the enzymolysis liquid after enzymolysis processing is passed through
Supernatant is taken after 10000rpm centrifugation, then vacuum freeze drying is configured to same concentrations using the borate buffer that pH is 8.3
Solution, be respectively adopted molecular cut off 10KDa, 3KDa, 1KDa ultra-filtration centrifuge tube processing enzymolysis liquid, to reach to molecule
Measure the purpose that different polypeptides is sieved.Molecular weight ranges > 10KDa, 10KDa~3KDa, 3KDa~1KDa, the < of acquirement
The different polypeptide fractions of 1KDa, are respectively measured its content of peptides.By the polypeptide group of the molecular weight ranges < 1KDa of preparation
Divide and collect and be freeze-dried, obtained dry product is re-dissolved in the borate buffer that pH is 8.3, uses gel chromatography
Column carries out column chromatography.Carry out the sodium chloride solution for the phosphate buffer 0.15M that column chromatographic eluate A phase is 0.05M, pH7.0；
Flow velocity 0.5mL/min；Loading volume 1mL (0.22 μm of membrane filtration).Active component is collected at 214nm, it will after repeatedly collecting
Different component freeze-drying, and measure molecular weight ranges and its content.
Chinese chestnut peptide beverage Starch Hydrolysis degree, protein degree, content of peptides and small peptide content (amino acid number 2-10) are surveyed
It is as shown in table 1 below to obtain result.
Table 1
| Starch Hydrolysis degree | Protein degree | Content of peptides | Small peptide content (amino acid number 2-10) | |
| Embodiment 1 | 90% | 21.8% | 13.2% | 2.8% |
| Embodiment 2 | 90% | 23.4% | 12% | 2% |
| Comparative example 1 | 23% | 4% | 1.6% | 0.6% |
| Comparative example 2 | 66% | 14% | 6.6% | 1.5% |
| Comparative example 3 | 0 | 2.2% | 0.9% | 0.2% |
| Comparative example 4 | 90% | 13% | 6% | 1.2% |
By upper table 1 it is found that hydrolyzing chestnut starch using two enzymes method, high-temperature resistant alpha-amylase and saccharification are passed through in front and back respectively
Enzyme hydrolysis, Starch Hydrolysis degree can be up to 90%；Chestnut starch after hydrolysis will not hinder the hydrolysis of protein, it can use egg
Protein in the white efficient hydrolysis Chinese chestnut of enzyme.Protein degree in embodiment 1,2 can be higher than 21%；Content of peptides
Not less than 12%；Small peptide content (amino acid number 2-10) is also not less than 2%, or even can finally be reached using neutral proteinase hydrolysis
2.8%.Compared to the hydrolysis for carrying out protein after the single enzymolysis starch of comparative example 1,2 again, the small peptide content in embodiment 1 is remote
Higher than comparative example 1,2.The hydrolysis without directly carrying out protein by Starch Hydrolysis in comparative example 3, protein hydrolysis
Degree, small peptide content and small peptide content, which are far smaller than in embodiment 1, hydrolyzes starch through two enzymes method.It forms sediment from the foregoing, it will be observed that reducing
The length of powder strand both can reduce the steric hindrance of starch, realize that the high efficiency of protein hydrolysis carries out.
Comparative example 4 is to first pass through two enzymes method starch is hydrolyzed, after protein is hydrolyzed by papain again,
Protein degree, content of peptides and small peptide content (amino acid number 2-10) be also it is relatively low, embody the specificity of enzyme, with
And the selection of protease cannot directly be exchanged by existing efficient protein enzyme.The present invention is in neutral proteinase and alkali protease
Under enzymatic hydrolysis, protein degree, content of peptides and small peptide content (amino acid number 2-10) can reach higher level.
Embodiment of above is merely illustrative of the technical solution of the present invention, rather than its limitations；Although referring to aforementioned implementation
Invention is explained in detail for mode, those skilled in the art should understand that: it still can be to aforementioned each
Technical solution documented by embodiment is modified or equivalent replacement of some of the technical features；And these are repaired
Change or replaces, the spirit and scope for each embodiment technical solution of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of preparation method of function chestnut drink, which comprises the following steps:
The pre-treatment of S1, Chinese chestnut: removing the peel the Chinese chestnut after over cleaning to obtain Chinese chestnut Seed, carries out again after Chinese chestnut Seed is crushed
Homogeneous；Calcium ion is added after the completion of homogeneous, and carries out deodorization and sterilization processing under high temperature；
S2, amylorrhexis: being adjusted to high temperature with the slashings chestnut after sterilization processing to deodorization and maintain temperature constant, and calcium chloride is added and stirs
8-10min is mixed, the rear Thermostable α-Amylase that is added sufficiently is digested to starch；Sugar is added after the completion of Thermostable α-Amylase enzymatic hydrolysis
Change enzyme sufficiently to digest to feed liquid；
S3, protein digestion: the feed liquid after carbohydrase is sufficiently digested is adjusted to protease optimum enzymolysis condition, after add albumen
Enzyme sufficiently digests feed liquid；
S4, enzyme deactivation: the product after protein digestion is subjected to destroy the enzyme treatment, is obtained by filtration after destroy the enzyme treatment rich in small peptide
Chestnut drink.
2. the preparation method of function chestnut drink according to claim 1, it is characterised in that: homogeneous described in S1 is in homogeneous tank
Middle progress；The specific steps of the homogeneous are as follows: smashed Chinese chestnut is transferred in homogeneous tank, keeps that pressure inside the tank is constant to be
30Mpa stirs 15min~20min；The condition of deodorization and sterilization processing described in S1 are as follows: at 140 DEG C, constant temperature handles 5~6s.
3. the preparation method of function chestnut drink according to claim 1, it is characterised in that: S2 amylase enzymolysis process in
It is carried out in Biochemical pot；The specific steps of S2 amylorrhexis are as follows: in the slashings Li Fangzhi Biochemical pot after deodorization and sterilization, adjust
It saves in tank to 96 DEG C of constant temperature, after the 0.01mol/L calcium chloride solution stirring 10min of 0.02wt% is added by Chinese chestnut quality；It adjusts
PH to 6.5, then the Thermostable α-Amylase that 0.08wt% enzyme activity is 4000U/g is added by Chinese chestnut quality and digests 70min；Adjust tank
Interior temperature is to 65 DEG C of constant temperature, after adjusting pH to 5.0, then the glucoamylase enzyme for being 100,000 U/g by Chinese chestnut quality addition 1.5wt% enzyme activity
Solve 120min.
4. the preparation method of function chestnut drink according to claim 1, it is characterised in that: egg described in S3 protein digestion
White enzyme is alkali protease or neutral proteinase.
5. the preparation method of function chestnut drink according to claim 4, it is characterised in that: S3 protein digestion it is specific
Step are as follows: adjust the temperature to 56 DEG C of constant temperature, pH is constant to 7.0, and 3200U/g albumen is added in the enzymolysis liquid after carbohydrase enzymatic hydrolysis
Enzyme activity is the neutral proteinase of 200,000 U/g；Enzymolysis time is 4.5h；
Or the specific steps of S3 protein digestion are as follows: adjust the temperature to 55 DEG C of constant temperature, pH is constant to 8.5, after carbohydrase enzymatic hydrolysis
Enzymolysis liquid in be added 3000U/g albumen enzyme activity be 200,000 U/g alkali protease；Enzymolysis time is 4h.
6. the preparation method of function chestnut drink according to claim 1, it is characterised in that: destroy the enzyme treatment described in S4 enzyme deactivation
Reaction condition be 120 DEG C at react 5~10min；It is described to be filtered into diatomite plate-frame filtering.
7. the preparation method of function chestnut drink according to claim 1, it is characterised in that: also through overrich after S4 enzyme deactivation
Contracting processing with it is filling；The concentration carries out in concentration tank；The concentration and filling specific steps are as follows: at concentration
Chestnut drink after reason through being obtained by filtration, then canned chestnut drink is made through filling, canned chestnut drink high-temp steam sterilizing obtains
To finished product.
8. the preparation method of function chestnut drink according to claim 6, it is characterised in that: before and after the concentration altogether
It is handled by three concentration tanks；After the concentration by be filtered into 0.1-0.3 μm fine ga(u)ge screen filter.
9. the preparation method of function chestnut drink according to claim 1, it is characterised in that: described in the pre-treatment of S1 Chinese chestnut
Cleaning is that clear water cleans three times repeatedly；Described crush is that three colloid mills are ground, and vibrating screen is equipped between adjacent twice colloid mill.
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