CN109813891A - A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chlorpromazine - Google Patents
A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chlorpromazine Download PDFInfo
- Publication number
- CN109813891A CN109813891A CN201711151227.6A CN201711151227A CN109813891A CN 109813891 A CN109813891 A CN 109813891A CN 201711151227 A CN201711151227 A CN 201711151227A CN 109813891 A CN109813891 A CN 109813891A
- Authority
- CN
- China
- Prior art keywords
- microballoon
- chlorpromazine
- line
- detection
- test strips
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention relates to chlorpromazine rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper preparation method and applications.The test strips are made of Fusion5 film, nitrocellulose filter and blotting paper three parts, by competition law principle, using time-resolved fluorescence microballoon as immune marker, carry out quickly and accurately quantitative detection to determined antigen.
Description
Technical field
The invention belongs to field of detection of food safety, and in particular to the detection method of the Harmful Residue in food, especially
Time-resolved fluoroimmunoassay chromatograph test strip of chlorpromazine quantitative detection and its preparation method and application.
Background technique
Chlorpromazine belongs to phenothiazines metabolin, and this drug mainly in liver metabolism, is also easy to produce medicament residue, remaining
There is the active metabolin of raw medicine can cause oligoleukocythemia and agranulocytosis for chlorpromazine and part, so as to cause human liver
Dirty, kidney lesion can also cause the complication etc. of eye.There is the raiser of certain countries and regions in consumption animal feeding
Middle addition chlorpromazine, leads to the residual for having chlorpromazine in relevant animal food, therefore periodically detects to chlorpromazine residual
The necessary means of monitoring as many countries.
Current immunochromatography (lateral flow immunoassay, LFIA) Rapid detection test strip is mostly with colloid
Gold, color latex microballoon or fluorescein are as marker.Based on the quick testing product of colloidal gold-labeled method exploitation, exist
The problems such as qualitative or sxemiquantitative, differences between batches are larger；Although color latex microballoon difference between batch improves to some extent, sensitivity is still
It is lower, it also can only qualitative or sxemiquantitative；Immunochromatography sensitivity based on fluorescein labelling technique is greatly improved, also can be into
Row quantitative detection, but due to containing higher autofluorescent background signal in sample, and stock displacement is smaller, can generate to detection
Large effect.
Time-resolved fluorescence (Time-resolved Fluorescence, TRF) is a kind of heterotope fluorescent marker,
Compared with common fluorescent, have stock displacement big, the features such as fluorescence lifetime is long, can effectively avoid the background in sample glimmering
The influence of the stray lights such as light and exciting light, therefore there is higher sensitivity and anti-interference ability compared to common fluorescent.
The present invention substitutes traditional sample pad, blood filter membrane, bonding pad using Fusion5, by original four layers even five layers
Membranous system simplifies, and develops the fast quantification immunity chromatography detection test paper for only needing trilamellar membrane structure, not only obtains production technology
Simplification has been arrived, and has effectively reduced batch internal difference and the difference between batch of product, has improved detection sensitivity, great practical value.
Summary of the invention
The purpose of the invention is to provide the time-resolved fluoroimmunoassay layer of novel energy rapid quantitative detection chlorpromazine
Analyse test strip and its preparation method and application.
One aspect of the present invention discloses chlorpromazine time-resolved fluoroimmunoassay chromatography quantitative testing test paper item, the test strips packet
Include Fusion5 film, nitrocellulose filter and blotting paper three parts.Nitrocellulose filter is located at centre, Fusion5 film and water suction
Paper is overlapped in nitrocellulose filter left and right ends respectively.Wherein, Fusion5 film is equipped with sample application zone and microballoon area, in microballoon area
It is loaded with time-resolved fluorescence microballoon；Nitrocellulose filter is equipped with detection line (T line) and nature controlling line (C line), and T line connects coating chlorine
Promazine antigen, C line are coated with anti-rabbit antibody.
It is an advantage of the invention that the content of chlorpromazine in tissue can be quickly detected.
The chlorpromazine time-resolved fluorescence microballoon, including detection microballoon and Quality Control microballoon, detection microballoon are pan coating
There is the fluorescent microsphere of chlorpromazine monoclonal antibody, Quality Control microballoon is the fluorescent microsphere that pan coating has rabbit-anti labelled protein.
Bright-coloured series elements compound is filled in the fluorescent microsphere；Preferably, which is europium chelating object；
Optimal, which can be Eu(TTA)3/ TOPO or Eu(TTA)3/Phen 。
The albumen can be cow's serum Y-globulin (BGG) or balf serum albumin (BSA).
The time-resolved fluorescence microspherulite diameter range is 100-1000 nm.
On the nitrocellulose filter, coated antigen is chlorpromazine antigen, coated anti-rabbit antibody on C line on T line.
The chlorpromazine time-resolved fluoroimmunoassay quantitative testing test paper bottom is equipped with plastic bottom board.
Second aspect of the present invention discloses the preparation side of chlorpromazine time-resolved fluoroimmunoassay chromatography quantitative testing test paper item
Method, comprising the following steps:
(1) prepared by Quality Control microballoon:
1. using biotinylated protein；
2. being coated with aldehyde group modified fluorescent microsphere using above-mentioned labelled protein；
(2) detects microballoon preparation
Aldehyde group modified fluorescent microsphere is coated with using the monoclonal antibody of chlorpromazine；
(3) blank kilocalorie is pasted
Using overlapped mode, first stickup nitrocellulose filter on the plastic bottom board with gum, then in cellulose nitrate
Blotting paper and Fusion5 film are pasted respectively in plain film left and right ends；
(4) sprays film
By the fluorescent microsphere of the fluorescent microsphere of rabbit-anti labelled protein and chlorpromazine monoclonal antibody using release buffer mixed diluting to one
Determine concentration, is sprayed onto the microballoon area of Fusion5 film；After chlorpromazine antigen and the dilution of anti-rabbit antibody, it is sprayed onto nitrocellulose filter respectively
T line and C line position；
(5) is dry and cuts.
By the above-mentioned kilocalorie drying for having sprayed reagent, slitting.
Step (4) in the release buffer used contain 10-15% sucrose, 3-10% trehaloses, 0. 5-1%N, 0-
Double trimethylsilyl acetamides (BSA), 0.1 one 0.5% gentamicins.
Step detects final concentration of 0.5-3 mg/mL of microballoon, Quality Control microballoon final concentration 0.05-0.2 after (4) diluting
mg/mL；0.5-3 mg/mL of T line antigen final concentration, 0.5-23mg/mL of C line antigen final concentration；C, T line spray film liquid amount
0.5-1.5 μ 1/cm, it is 2-10 μ l/cm that microballoon, which sprays film amount,.
Step (5) described in drying can in constant temperature oven or drying chamber, 37 DEG C dry 12-16 hours.
Third aspect present invention discloses the application of chlorpromazine time-resolved fluoroimmunoassay chromatography quantitative testing test paper item.
Test strips of the invention can measure biomolecule by competition law principle, use competition law mode suitable for all
Immunochromatography detection.
Chlorpromazine time-resolved fluoroimmunoassay chromatograph test strip high sensitivity of the invention, withinrun precision is up to 10% left side
The right side, betweenrun precision can detect whole blood, serum, blood plasma, urine specimen, 250 mg/dL hemoglobins, 500 up to 15% simultaneously
Mg/dL triglyceride, 10 mg/dL bilirubin on the detection of this test strips without influence, far more than most of immunochromatographies in the market
Quick diagnosis product.
Detailed description of the invention
Fig. 1 test strips structure schematic diagram of the present invention (1. plastic bottom boards, 2. Fusion5 films, 3. nitrocellulose filters, 4.
Blotting paper, 5. sample application zones, 6. microballoon areas, 7. T line areas, 8. C line areas).
Specific embodiment
1. the preparation of each ingredient of test strips
The preparation for preparing the coated Quality Control microballoon of biotin labeling gamma globulin (BGG) of 1.1 Quality Control microballoons
(1) the preparation of the BGG of biotin labeling
With 0.1 M NaCNBH3BGG(is purchased from Pel-Freez Biological) it is configured to l0 mg/mL solution, it uses
DMSO(dimethylformamide) configuration Biotin-X-X-NHS(N- HOSu NHS modified biological element, manufacturer: SIGMA,
Production number: B3295) solution is to 16.2 mg/mL, according to the amount general of 1 mg BBG albumen addition, 5.4 μ, 1 Biotin-X-X-NHS
Biotin-X-X-NHS liquid is added in BGG solution, is uniformly mixed and is stood overnight at 4 DEG C.It is removed using dialysis free
Unreacted biotin, dialyzate are biotinylated protein elution buffer (0.1 M Tris, 0.3 M Nacl, 0.005 M
EDTA-Na-2H20, pH8.0).Dialysis finishes, and BCA method measures protein concentration.
(2) aldehyde group modified fluorescent microsphere is coated with using above-mentioned labelled protein
The above-mentioned (1) middle dialysis of Tween-20 solution, 2 mg that 6 μ l 20% are added in the fluorescent microsphere aldehyde group modified to 10mL obtains
Biotinylated protein and 16 μ l NaCNBH3, (25 mg/mL, the MES buffer of 0.05 M pH6.0, now match
It is current), adding 0.1 M pH6.0 MES to total volume is 400 μ l, and 37 DEG C are protected from light 48 h of incubation.The Gly solution of 40 μ l is added
(75 mg/mL, the MES buffer of 0.05 M pH6.0), 37 DEG C are protected from light 2 h of revolving reaction.Add 250 μ l N, 0- bis- three
First silicon substrate acetamide (200 mg/mL, the MES buffer of 0.05 M pH6.0) solution.37 DEG C are protected from light 16 h of revolving reaction.
4 DEG C of 13000 rpm is centrifuged 30 minutes.Supernatant is abandoned, is washed again twice with the MES buffer of 1 mL pH6.0.With 1 mL, 0.05 M
HEPES buffer solution (the 0.05 M HEPES, 0.3 M Nacl, 0.025 M EDTA-Na-2H of pH8.020,1.6% N, 0-is bis-
Trimethylsilyl acetamide (BSA), 0.1% Dextran, 0.1%Tween-20,0.3745% Triton X- 405,0.0l% celebrating are big
Mycin, 0.05 % Proclin) suspend (its final concentration of l0 mg/mL).
(3) Quality Control microballoon working solution is prepared
According to need of work, (2) respective concentration will be diluted to by middle suspension using the HEPES buffer solution of 0.05M pH 8.0, packing is protected
It deposits.
The preparation of 1.2 detection microballoons
Tween-20 solution, the 2 mg chlorpromazine monoclonal antibodies of 6 μ l 20% are added in the fluorescent microsphere aldehyde group modified to 10 mg
And 16 μ l NaCNBH3(25 mg/mL, the MES buffer of 0.05 M pH6.0 are ready-to-use), adds 0.1 M
PH6.0 MES is 400 μ l to total volume, and 37 DEG C are protected from light 48 h of incubation.Gly solution (75 mg/mL, 0.05 M of 40 μ l are added
The MES buffer of pH6.0), 37 DEG C are protected from light 2 h of revolving reaction.Add 250 μ l BSA(200 mg/mL, 0.05 M pH6.0
MES buffer) solution.37 DEG C are protected from light 16 h of revolving reaction.4 DEG C of 13000 rpm is centrifuged 30 minutes.Supernatant is abandoned, with 1
The MES buffer of mL pH6.0 is washed twice again.With the HEPES buffer solution of 1 mL, 0.05 M pH8.0 (0.05 M HEPES, 0.3
M NaC1,0.25 M EDTA-Na-2H20,1.6% BSA, 0.1% Dextran, 0.1% Tween-20,0.3745% Triton
X-405,0.01% gentamicin, 0.05% Proclin) suspend (its final concentration of 10 mg/mL).
The configuration of 1.3 microspheres solutions
With release buffer (contain 20% sucrose, 5% trehalose, 0.5% BSA, 0.2% gentamicin) by Quality Control microballoon and detection
Microballoon is configured to time-resolved fluorescence microballoon mixed liquor, and final concentration of 0.2 mg/mL of Quality Control microballoon detects the final concentration of l of microballoon
mg/mL。
The preparation of 1.4 detection lines (T line) solution
Chlorpromazine antigenic compound is diluted to 0.5 mg/mL with 0.01 M PB solution.
The preparation of 1.5 nature controlling lines (C line) solution
Streptavidin is diluted to 0.5 mg/mL with 0.01 M PB solution.
2. the preparation of test strips
2.1 blank kilocalories are pasted
According to the film combination of attached drawing 1, nitrocellulose filter 3 is located at centre, and Fusion5 film 2 is overlapped in respectively with blotting paper 4
Nitrocellulose filter left and right ends are pasted on the plastic bottom board with gum.
2.2 spray films
T is sprayed in the T line 7 in Fig. 1 respectively, 8 position of C line, and C line solution, C, it is 0.8 μ l/cm that T line, which sprays film liquid amount, 6 in Fig. 1
It sets and sprays microspheres solution, it is 4 μ l/cm that microspheres solution, which sprays film amount,.
2.3 drying
By step (2) in sprayed the chlorpromazine of reagent and be stuck in constant temperature oven 37 DEG C greatly and dry 16 hours.
2.4 slitting
The chlorpromazine kilocalorie of drying is cut into the paper slip of 4 mm width to get chlorpromazine test strips are arrived.
3. detecting instrument principle and application
3.1 measuring instrument principles: condenser lens is located at exciting light into 90 degree of directions, generates the sample in sample detection pond
Fluorescence signal line focus lens focus, then photomultiplier tube is reached after grating beam splitting, photomultiplier tube converts light signals into
Electric signal is finally sent to A/D converter and is handled.The laser light source uses diode pumped solid state laser, output
Power is 20 mW, and launch wavelength is 375 ± 5 nm.Fiber power of the exciting light after conducting fiber coupling is 5 mW or more.
Ultraviolet quartz glass is selected in sample detection pond.Grating is set as that the light for obtaining that wavelength is 440 ± 5nm can be divided.
3.1 detector applications: the extract after pretreatment of the edible animal tissue containing chlorpromazine is packed into sample inspection
It surveys in pond, laser light source issues the laser that wavelength is 375 ± 5nm, conducts after conducting fiber coupling to sample detection pond, and wear
Sample to be tested is crossed, the sample in sample detection pond generates fluorescence after absorbing exciting light, and the fluorescence signal line focus lens of generation are poly-
Coke, then through grating beam splitting, the light that wavelength is 440 ± 5 nm is obtained, photomultiplier tube is reached, photomultiplier tube turns optical signal
Become electric signal, while input a/d converter after electric signal is amplified, finally export and give reading plotter processing, passes through reading dress
Result can directly be read by setting.
4. the quantitative detection of test strips
4.1 draw standard curve
Be added in the chlorpromazine test strips sample application zone prepared various concentration chlorpromazine antigen standard (take five it is different
Concentration, respectively 0,10,30,90,270ng/mL, each concentration set 5 repetitions), sample-loading buffer is added dropwise, and (PBS contains 1.6%
BSA, 0.1% Tween-20, preservative), after film layer is analysed 10 minutes, instrument reading C, T line signal,
Experimental result and analysis are shown in Table 1:
1 chlorpromazine standard items testing result of table
Standard curve is drawn with antigen standard concentration and the signal averaging of measurement, using four parameter fitting modes, standard is bent
Line number evidence is shown in Table 2.
2 chlorpromazine quantitative measurement standard curve data of table
| SPE standard items (ng/mL) | 0 | 10 | 30 | 90 | 270 |
| Reading | 18.918 | 12.48 | 8.536 | 3.706 | 1.44 |
4.2 sample detection
Sample to be tested is sequentially added in the sample application zone of chlorpromazine test strips, sample-loading buffer is added dropwise, after film layer is analysed 10 minutes, instrument
Device reads C, T line signal.According to step (1) in standard curve calculate sample to be tested in chlorpromazine antigen concentration.
5. test strips performance test
10 times crowdes of internal difference CV of 2 concentration samples are measured, experimental result is shown in Table 3, the experimental results showed that, batch interior precision of test strips
Degree is respectively less than 11%.
3 test strips of table batch internal difference
0-500 ng/mL can reach using chlorpromazine quantitative testing test paper detection range prepared by the method, sensitivity exists
For 10 ng/mL hereinafter, withinrun precision is up to 11% or so, betweenrun precision can detect tissue, whole blood, blood up to 15% simultaneously
Clearly, plasma sample, 250 mg/dL hemoglobins, 500 mg/dL triglycerides, 10 mg/dL bilirubin on this detection without influence,
Far more than most of immunochromatography quick diagnosis products in the market.
Claims (10)
1. chlorpromazine rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper, it is characterised in that: including Fusion5 film,
Nitrocellulose filter and blotting paper three parts, nitrocellulose filter are located at centre, and Fusion5 film is overlapped in respectively with blotting paper
There are sample application zone and microballoon area in nitrocellulose filter left and right ends on Fusion5 film, and microballoon area is loaded with chlorpromazine monoclonal antibody
Time-resolved fluorescence microballoon；There are detection line and nature controlling line on nitrocellulose filter, is coated with chlorpromazine antigen, nature controlling line in detection line
Upper coating anti-rabbit antibody.
2. test strips as described in claim 1, it is characterised in that: the time-resolved fluorescence microballoon includes detection microballoon and matter
Microballoon is controlled, detection microballoon is the fluorescent microsphere that pan coating has chlorpromazine monoclonal antibody, and Quality Control microballoon is that pan coating has rabbit-anti label
The fluorescent microsphere of albumen.
3. test strips as claimed in claim 1 or 2, it is characterised in that: the particle size range of the time-resolved fluorescence microballoon is
100 - 1000 nm 。
4. test strips as described in claim 1, it is characterised in that: being coated with chlorpromazine detection in the detection line with antigen is chlorine
The conjugates that promazine and carrier mass are formed；Two anti-rabbit antibody are coated in detection line.
5. the preparation method of test strips as described in claim 1, preparation step are as follows:
(1) prepared by Quality Control microballoon:
1. using biotinylated protein；
2. being coated with aldehyde group modified fluorescent microsphere using above-mentioned labelled protein；
(2) detects microballoon preparation: aldehyde group modified fluorescent microsphere is coated with using the monoclonal antibody of chlorpromazine；
(3) blank kilocalorie is pasted: nitrocellulose filter being pasted among plastic bottom board, Fusion5 film is taken respectively with blotting paper
It is connected to nitrocellulose filter left and right ends；
(4) sprays film: the fluorescent microsphere of the fluorescent microsphere of rabbit-anti labelled protein and chlorpromazine monoclonal antibody is mixed using release buffer
It is diluted to a certain concentration, is sprayed onto the microballoon area of Fusion5 film；After chlorpromazine antigen and the dilution of anti-rabbit antibody, it is sprayed onto nitric acid respectively
The T line and C line position of cellulose membrane；
(5) dry and slitting.
6. preparation method as claimed in claim 5, it is characterised in that: (4) middle release buffer group becomes 10-15% sugarcanes to step
Sugar, 3-10% trehaloses, 0. 5-1% N, the bis- trimethylsilyl acetamides (BSA) of O-, 0.1-0.5% gentamicin.
7. preparation method as claimed in claim 5, it is characterised in that: step detects microballoon final concentration of 0.5-after (4) diluting
3mg/mL, 0.05-0.3 mg/mL of Quality Control microballoon final concentration；0.5-3mg/mL of T line antigen final concentration, C line antigen final concentration
0. 5 - 3 mg/mL；It is 0.5-1.5 μ 1/cm that C, T line, which spray film liquid amount, and it is 2-10 μ l/cm that microballoon, which sprays film amount,.
8. preparation method as claimed in claim 5, it is characterised in that: (5) middle drying condition is 37 DEG C of drying 12-16 to step
Hour.
9. the chlorpromazine rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper as described in claim 5 claim,
It is characterized by: test strips bottom is equipped with plastic bottom board.
10. the application of test strips as described in claim 1, it is characterised in that: the test strips are used for using competition law mode
The quantitative detection of immunochromatography.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711151227.6A CN109813891A (en) | 2017-11-18 | 2017-11-18 | A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chlorpromazine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711151227.6A CN109813891A (en) | 2017-11-18 | 2017-11-18 | A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chlorpromazine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109813891A true CN109813891A (en) | 2019-05-28 |
Family
ID=66597665
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711151227.6A Withdrawn CN109813891A (en) | 2017-11-18 | 2017-11-18 | A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chlorpromazine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109813891A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070167853A1 (en) * | 2002-01-22 | 2007-07-19 | Melker Richard J | System and method for monitoring health using exhaled breath |
| CN101424688A (en) * | 2008-11-20 | 2009-05-06 | 上海交通大学 | Method for detecting chlorpromazine by colloidal gold immune chromatography test |
| CN102236012A (en) * | 2010-04-21 | 2011-11-09 | 深圳出入境检验检疫局食品检验检疫技术中心 | Time resolved fluoroimmunoassay kit for detecting chlorpromazine and detecting method thereof |
| CN102539750A (en) * | 2011-11-25 | 2012-07-04 | 佛山市中南农业科技有限公司 | Time-resolved immunoassay kit for detecting chlorpromazine residues and detection method thereof |
| CN103792357A (en) * | 2012-11-05 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip |
| CN104655835A (en) * | 2013-11-20 | 2015-05-27 | 南京亿特生物科技有限公司 | Immune colloidal gold detect card of chlorpromazine, and preparation method thereof |
| CN106568946A (en) * | 2015-10-13 | 2017-04-19 | 镇江亿特生物科技发展有限公司 | immunogold detection card of propiconazole, and preparation method thereof |
-
2017
- 2017-11-18 CN CN201711151227.6A patent/CN109813891A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070167853A1 (en) * | 2002-01-22 | 2007-07-19 | Melker Richard J | System and method for monitoring health using exhaled breath |
| CN101424688A (en) * | 2008-11-20 | 2009-05-06 | 上海交通大学 | Method for detecting chlorpromazine by colloidal gold immune chromatography test |
| CN102236012A (en) * | 2010-04-21 | 2011-11-09 | 深圳出入境检验检疫局食品检验检疫技术中心 | Time resolved fluoroimmunoassay kit for detecting chlorpromazine and detecting method thereof |
| CN102539750A (en) * | 2011-11-25 | 2012-07-04 | 佛山市中南农业科技有限公司 | Time-resolved immunoassay kit for detecting chlorpromazine residues and detection method thereof |
| CN103792357A (en) * | 2012-11-05 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip |
| CN104655835A (en) * | 2013-11-20 | 2015-05-27 | 南京亿特生物科技有限公司 | Immune colloidal gold detect card of chlorpromazine, and preparation method thereof |
| CN106568946A (en) * | 2015-10-13 | 2017-04-19 | 镇江亿特生物科技发展有限公司 | immunogold detection card of propiconazole, and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| BIAO HUANG 等: ""Establishment and Validation of a Time-Resolved Fluoroimmunoassay for Chlorpromazine"", 《FOOD ANALYTICAL METHODS》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103792357A (en) | Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip | |
| CN102192983B (en) | Time-resolved immunoflourometric chromatographic test strip for quantitative detection as well as preparation method and application thereof | |
| CN106872420B (en) | Kit and method for time-resolved fluorescence quantitative detection of microalbuminuria | |
| CN102680692B (en) | Immunochromatographic quantitative test reagent based on near-infrared fluorescent marker | |
| CN202149903U (en) | Time-resolved fluorescence immune chromatography quantitative detection test strip for C-reactive protein | |
| CN108398562A (en) | Cystatin C fluorescent micro-ball immune chromatography quantitative testing test paper item and test card | |
| CN105785030A (en) | Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E) | |
| JP7352831B2 (en) | Immunochromatography test piece, measurement kit, and measurement method | |
| CN207248894U (en) | Bladder chalone C time resolution detection card and kit | |
| CN101999076A (en) | Immunoassay analyzer and immunoassay method | |
| CN108333368A (en) | The kit and preparation method of calprotectin in a kind of detection human faecal mass | |
| CN108088994A (en) | A kind of hollow core shell nanoparticles and preparation method, test strips and test method | |
| JP4562854B2 (en) | Chromatographic measurement method | |
| CN110133279A (en) | A kind of joint inspection colloidal gold strip detecting transgenosis BT albumen and CP4-EPSPS albumen | |
| CN109813900A (en) | A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chloramphenicol | |
| CN202166649U (en) | Time-resolved fluoroimmunoassay quantitative detecting test strip for neopterin | |
| CN101285839B (en) | Quick detection method for newcastle disease virus and its immune colloidal gold test card | |
| JP2007240321A (en) | Quantitative detection method of specimen | |
| JP7510620B2 (en) | Method for producing allergen-immobilized carrier and method for detecting allergen-specific antibodies | |
| CN108535495A (en) | A kind of time-resolved fluoroimmunoassay chromatograph test strip and preparation method quantitatively detecting CYFRA21-1 in blood | |
| CN105301248A (en) | Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine | |
| CN109813892A (en) | Anarexol rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper | |
| CN109813890A (en) | Lincomycin rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper | |
| CN109813891A (en) | A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chlorpromazine | |
| CN109813899A (en) | Ceftiofur time-resolved fluoroimmunoassay chromatographs quantitative testing test paper item |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190528 |
|
| WW01 | Invention patent application withdrawn after publication |