CN109709169A - The method of electrochemical luminescence detection dopamine based on boron-nitrogen compound - Google Patents
The method of electrochemical luminescence detection dopamine based on boron-nitrogen compound Download PDFInfo
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Abstract
The method for the electrochemical luminescence detection dopamine that the invention discloses a kind of based on boron-nitrogen compound, carries out as steps described below: the sample to be tested containing dopamine is uniformly blended in phosphate buffer；Boron nitrogen composite powder is uniformly laid with and is fixed on carbon paper side, polyaniline is uniformly laid in carbon paper reverse side, working electrode is used as after drying, electrochemical luminescence intensity detection is carried out using three-electrode system, the electrochemical luminescence intensity of measurement is compareed with equation of linear regression, you can learn that the dopamine concentration in solution, and then obtain the content of dopamine in sample to be tested.The present invention is that a kind of detection limit is lower, the range of linearity is wider, sensitivity is higher, the detection method of the simple dopamine of the instrument based on boron-nitrogen compound electrochemical luminescence, and the cost for detecting dopamine is low, high sensitivity, easy to operate, and selectivity is good.
Description
Technical field
The present invention relates to a kind of electrochemical luminescence detection fields.More particularly to a kind of electrochemistry hair based on boron-nitrogen compound
The method of light detection dopamine.
Background technique
In illumination field, the research of organic material is increasingly subject to the attention of people.Organic compound have it is many kinds of,
The advantages that adjustability is good, rich in color, excitation purity is high and MOLECULE DESIGN is relatively flexible, so having in luminescent material fine
Application prospect.Nowadays boron azepine chemistry is increasingly becoming the important branch in organic semiconductor luminescent material, in conjugation point
Sulphur is introduced in sub- skeleton, nitrogen and oxygen can assign conjugated molecule different photoelectric properties, especially substitute carbon with carbon-to-nitrogen double bon
Carbon double bond can generate the different analogs of benzene.The aromatic compound of boron N doping has in terms of the synthesis and in terms of physicochemical properties
Good development, but the application in terms of luminescent material is in the brand-new stage.
Electrogenerated chemiluminescence (Electrochemiluminescence, abbreviation ECL) be three kinds of typical chemiluminescences it
One, it is the product that chemiluminescence method and electrochemical method be combined with each other, it is not only remained possessed by chemiluminescence method
Advantage, such as the range of linearity is wide, high sensitivity, observation is convenient and instrument is simple, at the same have again many chemiluminescence methods without
The advantage of method analogy, such as stable reagent, favorable reproducibility, the easy and some reagent of control may be reused.It is excellent using its
Point and characteristic, which are fabricated to sensor and apply it to context of detection, wide prospect.
Dopamine (dopamine, DA), chemical name 4- (2- ethylamino-) benzene -1,2- diphenol, chemical formula C8H11O2N, outside
Seeing is the white or glossiness crystalline solid of off-white color, soluble easily in water.Dopamine is a kind of is present in nerve fiber and body fluid
Catecholamines Neurotransmitters in Blood is the material base of brain function.Its content distribution of specific region in human brain affects hypophysis
The coordination of endocrine dysfunction, and it is directly related with nervous activity.Intracerebral dopamine neuronal functional disturbance be schizophrenia and
The major reason of Parkinson's disease.In addition, dopamine also has the function of excited heart, increases renal blood flow, it is scarce for treating
Hemorrhagic, cardiogenic and infectious shock.Therefore, carry out the research of dopamine analysis method in nervous physiology function, medical diagnosis on disease
And quality control of related drugs etc. important in inhibiting.
There are no the precedents in terms of the content detection that boron-nitrogen compound is applied to dopamine in the prior art.
Summary of the invention
For the deficiencies in the prior art, the purpose of the present invention is to provide a kind of electrifications based on boron-nitrogen compound
A kind of method for learning the detection dopamine that shines, it is desirable to provide detection limit is lower, the range of linearity is wider, the higher dopamine of sensitivity
Detection method makes sensor with characteristic using the advantages of electrochemical luminescence, realizes the accurate detection of DOPAMINE CONTENT IN RABBIT.
The present invention is achieved by the following technical solutions:
A method of the electrochemical luminescence based on boron-nitrogen compound detects dopamine, carries out: will contain as steps described below
There is the sample to be tested of dopamine to be uniformly blended in phosphate (PBS) buffer；Boron nitrogen composite powder is uniformly laid with simultaneously
It is fixed on carbon paper side, is uniformly laid with polyaniline in carbon paper reverse side, working electrode is used as after drying, utilizes three-electrode system
Electrochemical luminescence intensity detection is carried out, the electrochemical luminescence intensity of measurement is compareed with equation of linear regression, you can learn that
Dopamine concentration in solution, and then obtain the content of dopamine in sample to be tested；
Wherein, electrochemical luminescence intensity and dopamine concentration logarithm (log) carry out linear naturalization, obtain corresponding linear
Regression equation y (a.u.)=100.21x+6.74 (R2=0.99), y is the corresponding electrochemical luminescence intensity of sample to be tested, and x is more
Bar amine concentration logarithm (log), dopamine concentration range are 5.226 × 10-4mol/L-0.4mol/L。
In the above-mentioned technical solutions, the test condition of electrochemistry are as follows: photomultiplier tube high pressure 900V, sweep interval be-
2.7V to -1V, initial potential are -1V, sweep speed 80mv/s.
In the above-mentioned technical solutions, the specific production method of the working electrode are as follows: carbon paper is taken to be cut into (2-4) cm* (4-
6) boron nitrogen composite powder is uniformly layered on carbon paper side by cm size, and laying width is 0.8cm-1.2cm, then with 5%
Nafion dries after being fixed；It is cut into after carbon paper reverse side is equally uniformly layered on side with polyaniline, dries multiple
The electrode of (2-4) cm* (0.4-0.6) cm.
In the above-mentioned technical solutions, the polyaniline is prepared in accordance with the following steps:
It weighs 2-4gPVA (polyvinyl alcohol (98%)), 60-80ml water is added and is placed in magnetic stirring apparatus at 90-120 DEG C
After heating stirring is completely dissolved to PVA, 1-3gHCL, 2-4gLAS (linear alkyl benzene sulfonic acid (96%)) and 1- are sequentially added
The mixed solution of 2g ammonium persulfate and 10ml deionized water, mixing time 3-4h is added dropwise in 1.5g aniline after mixing evenly.
In the above-mentioned technical solutions, the concentration of the solution to be checked of dopamine based on the equation of linear regression obtained is respectively
5.226E-4M, 0.10M, 0.26M, 0.31M and 0.40M.
In the above-mentioned technical solutions, electro-chemical test is carried out using CHI660B type electrochemical workstation, using three electrode bodies
System, platinum filament are used as to electrode, and Ag/AgCl is as reference electrode；The specific production method of working electrode are as follows: carbon paper is taken to be cut into
Boron nitrogen composite powder is uniformly layered on carbon paper side by (2-4) cm* (4-6) cm size, and laying width is 0.8cm-1.2cm, so
It is dried after being fixed afterwards with 5% nafion；It is cut out after carbon paper reverse side is equally uniformly layered on side with polyaniline, dries
It is cut into the electrode of multiple (2-4) cm* (0.4-0.6) cm.
The present invention also provides application of the boron-nitrogen compound in detection dopamine.
The present invention is that a kind of detection limit is lower, the range of linearity is wider, sensitivity is higher, is based on boron-nitrogen compound electrochemistry
The detection method of the simple dopamine of luminous instrument.Using the advantages of electrochemical luminescence with characteristic make sensor, and will
Its detection for being applied to dopamine.Electrochemical luminescence is the product that chemiluminescence method and electrochemical method be combined with each other, it is not
Advantage possessed by chemiluminescence method is only remained, such as the range of linearity is wide, high sensitivity, observation is convenient and instrument is simple,
The advantage for having many chemiluminescence methods incomparable again simultaneously, such as stable reagent, favorable reproducibility, the easy and some examinations of control
Agent may be reused etc..Be fabricated to sensor using its advantage and characteristic and apply it to context of detection have it is wide
Prospect.
The working principle of the invention is: using three-electrode system (working electrode, reference electrode, to electrode), working electrode
It is studied object, the effect of reference electrode is the current potential of determining working electrode, and the effect to electrode is to constitute circuit conduction
Electric current.Tested substance (dopamine) is attracted by working electrode, and oxidation or reduction reaction occur near working electrode surface
Excitation state is generated in reaction process Deng consuming, excitation state is unstable to return to ground state, can discharge during returning to ground state
Energy out, and this part energy is released in the form of photon.By whether the light intensity of photon can be detected, to measure this object
Matter, and according to the strong and weak concentration that can deduce the substance in a certain range of luminous signal.
For the present invention using boron-nitrogen compound as illuminator, phosphate solution (pH=7) is buffer solution, working electrode production side
For method as described above, platinum electrode is to electrode, Ag/AgCl is reference electrode, the electric grade system of composition three, the chemiluminescent method of electricity consumption
Detect dopamine；The detection range of this method is 5.226 × 10-4- 0.4mol/L, the present invention detect the method cost of dopamine
Low, high sensitivity, easy to operate, selectivity is good.
Detailed description of the invention
Fig. 1 is the manufacturing process schematic diagram of working electrode of the present invention.
Fig. 2 is the structural schematic diagram of electrochemical luminescence detecting pool of the present invention, and it is Ag/AgCl that wherein WE, which is working electrode, RE,
Reference electrode, CE are to electrode (using platinum electrode as to electrode).
Fig. 3 is that the boron nitrogen compound (being represented with R) that the present invention uses and dopamine (represent) electrochemical luminescence principle with D and show
It is intended to.
Fig. 4 is the voltage and the Relationship of Light intensity curve graph of the compound analyte detection dopamine of boron nitrogen in the present invention.
Fig. 5 is the electrochemical luminescence curve (a cycle) of dopamine solution to be checked under various concentration, and wherein a to e's is dense
Degree is respectively a=5.226E-4M, b=0.10M, c=0.26M, d=0.31M, e=0.40M.
Fig. 6 is light intensity in the present invention with the equation of linear regression curve graph of dopamine concentration；X represents the concentration of dopamine, y
Represent the corresponding electrochemical luminescence intensity (light intensity) of dopamine solution to be checked of various concentration.
Fig. 7 is the obtained curve of stability test of electrochemical luminescence reaction in the present invention.
Fig. 8 is to carry out dopamine (DA) solution to be checked, the ascorbic acid that electrochemiluminescence assay obtains with control variate method
(AA) the anti-interference comparison diagram of solution, uric acid (UA) solution and glucose (G) solution.
Specific embodiment
Present invention is further described in detail with specific embodiment with reference to the accompanying drawing.Under it should be understood that
State embodiment be it is illustrative, be not restrictive, cannot be limited the scope of protection of the present invention with following embodiments.
Hydrochloric acid (HCL) dopamine powder used in the examples unifies Science and Technology Ltd. purchased from the Tianjin sky over the river, uses
Magnetic stirring apparatus, that II type Electrochemiluminescprocess process system multifunctional chemiluminescence detector of MPI-E is purchased from Chinese Xi'an is auspicious
The company of stepping；Boron-nitrogen compound is a kind of " simple height in Patent No. " CN201811165383.2 ", patent name according to Liu Xuguang
Method preparation described in the method for effect synthesis boron azepine aromatic hydrocarbons ".
A method of the electrochemical luminescence based on boron-nitrogen compound detects dopamine, the specific steps are that；
1) production of working electrode
It takes carbon paper to be cut into 3cm*5cm size, boron nitrogen composite powder is uniformly layered on carbon paper side, being laid with width is
1cm dries after being then fixed with 5%nafion；It is equally uniformly layered on side with polyaniline in carbon paper reverse side, it will after drying
Its electrode for being cut into 10 3cm*0.5cm is spare；5 working electrodes are only used in the present embodiment, remaining is spare；
Wherein, polyaniline is prepared in accordance with the following steps:
It weighs 3gPVA (polyvinyl alcohol (98%)), 70ml water is added and is placed in magnetic stirring apparatus the heating stirring at 110 DEG C
After being completely dissolved to PVA, 2gHCL, 3gLAS (linear alkyl benzene sulfonic acid (96%)) and 1.3g aniline are sequentially added, stirring is equal
The mixed solution of 2g ammonium persulfate and 10ml deionized water, mixing time 3h, the during which stirring of magnetic stirring apparatus are added dropwise after even
Pond top prevents moisture from evaporating with diaphragm seal, obtains blackish green thick polyaniline；
2) other agents useful for same
0.1mol/L phosphate (PBS) buffer of pH=7 is prepared as supporting electrolyte；
3) preparation of the dopamine of various concentration solution to be checked
Different amounts of hydrochloric acid (HCL) dopamine powder is mixed with deionized water, every time in the NaOH of equivalent and hydrochloric acid
(HCL) HCL in dopamine solution so that dopamine solution is in neutrality, obtain concentration be respectively 5.226E-4M, 0.10M,
The dopamine of 0.26M, 0.31M and 0.40M solution to be checked；
4) sample detection
II type Electrochemiluminescprocess process system multifunctional chemiluminescence detector of MPI-E and electrochemical analyser are for examining
The ECL in entire experiment is surveyed, using boron-nitrogen compound as illuminator, phosphate solution (pH=7) is buffer solution, working electrode system
Make method as described above, platinum electrode is to electrode, Ag/AgCl is reference electrode, and the electric grade system of composition three uses cyclic voltammetry
Photomultiplier tube is set as 900V, and sweep interval is that -2.7V arrives -1V, and initial potential is -1V, sweep speed 80mv/s, difference
Be added hydrochloric acid (HCL) dopamine solution of configured various concentration, observe CV curve and light intensity curve, and to the data obtained into
Row fitting, the different light intensity according to corresponding to various concentration, to draw linear curve, and by electrochemical luminescence intensity and dopamine
Log concentration value (log) carries out linear naturalization, obtains corresponding equation of linear regression-y (a.u.)=- 100.21x+6.74 (R2
=0.99), y is the corresponding electrochemical luminescence intensity of dopamine solution to be checked of various concentration, and x is dopamine concentration logarithm
(log)。
According to above-mentioned test case, electrochemical luminescence intensity can be obtained and the linear of dopamine concentration logarithm (log) returns
Change, sample to be tested is dispersed in PBS solution, and the voltage at the place use -2.1V is detected (the i.e. inspection of electrochemiluminescence intensity
Survey), the electrochemiluminescence intensity of measurement is compareed with linear naturalization equation, you can learn that dopamine concentration to be measured, into
And obtain the content of dopamine in sample to be tested.
Dopamine (DA) solution, ascorbic acid (AA) solution, uric acid of compound concentration identical (being 0.1mol/L) respectively
(UA) solution and glucose (G) solution, carry out electrochemiluminescence assay with control variate method, to detect the anti-interference of them
Property, test result is as shown in Figure 8, it can be seen that the anti-interference of dopamine is much larger than other components, and the present invention utilizes electrochemistry
The detection method reliability that luminous intensity detects DOPAMINE CONTENT IN RABBIT is high.
Illustrative description has been done to the present invention above, it should explanation, the case where not departing from core of the invention
Under, any simple deformation, modification or other skilled in the art can not spend the equivalent replacement of creative work equal
Fall into protection scope of the present invention.
Claims (10)
1. a kind of method of the electrochemical luminescence detection dopamine based on boron-nitrogen compound, which is characterized in that as steps described below
It carries out: the sample to be tested containing dopamine is uniformly blended in phosphate buffer；Boron nitrogen composite powder is uniformly spread
And if be fixed on carbon paper side, be uniformly laid with polyaniline in carbon paper reverse side, after drying be used as working electrode, utilize three electrodes
System carries out electrochemical luminescence intensity detection, and the electrochemical luminescence intensity of measurement is compareed with equation of linear regression
Know the dopamine concentration in solution, and then obtains the content of dopamine in sample to be tested；
Wherein, electrochemical luminescence intensity and dopamine concentration logarithm (log) carry out linear naturalization, obtain corresponding linear regression
Equation y (a.u.)=100.21x+6.74 (R2=0.99), y is the corresponding electrochemical luminescence intensity of sample to be tested, and x is dopamine
Log concentration value (log), dopamine concentration range are 5.226 × 10-4mol/L-0.4mol/L。
2. the method for the electrochemical luminescence detection dopamine according to claim 1 based on boron-nitrogen compound, feature exist
In the test condition of electrochemistry are as follows: photomultiplier tube high pressure 900V, sweep interval are that -2.7V arrives -1V, and initial potential is -1V,
Sweep speed is 80mv/s.
3. the method for the electrochemical luminescence detection dopamine according to claim 1 based on boron-nitrogen compound, feature exist
In the specific production method of the working electrode are as follows: take carbon paper to be cut into (2-4) cm* (4-6) cm size, by boron nitrogen compound
Powder is uniformly layered on carbon paper side, and laying width is 0.8cm-1.2cm, dries after being then fixed with 5%nafion；In carbon
Paper reverse side is equally uniformly layered on side with polyaniline, and the electrode of multiple (2-4) cm* (0.4-0.6) cm is cut into after drying.
4. the method for the electrochemical luminescence detection dopamine according to claim 3 based on boron-nitrogen compound, feature exist
In the polyaniline is prepared in accordance with the following steps:
2-4gPVA is weighed, it is completely molten to PVA that addition 60-80ml water is placed in magnetic stirring apparatus the heating stirring at 90-120 DEG C
Xie Hou sequentially adds 1-3gHCL, 2-4gLAS and 1-1.5g aniline, be added dropwise after mixing evenly 2g ammonium persulfate and 10ml go from
The mixed solution of sub- water, mixing time 3-4h.
5. the method for the electrochemical luminescence detection dopamine according to claim 1 based on boron-nitrogen compound, feature exist
In, the concentration of the solution to be checked of dopamine based on the equation of linear regression obtained be respectively 5.226E-4M, 0.10M, 0.26M,
0.31M and 0.40M.
6. the method for the electrochemical luminescence detection dopamine according to claim 5 based on boron-nitrogen compound, feature exist
In the dopamine solution to be checked is prepared in accordance with the following steps: different amounts of Dopamine hydrochloride powder is mixed with deionized water,
A series of differences are obtained so that dopamine solution is in neutrality with the HCL in the NaOH of equivalent and in Dopamine hydrochloride solution every time
The dopamine of concentration solution to be checked, concentration range are 5.226 × 10-4mol/L-0.4mol/L。
7. the method for the electrochemical luminescence detection dopamine according to claim 1-6 based on boron-nitrogen compound,
It is characterized in that, electro-chemical test is carried out using CHI660B type electrochemical workstation, using three-electrode system, platinum filament conduct pair
Electrode, Ag/AgCl is as reference electrode；The specific production method of working electrode are as follows: carbon paper is taken to be cut into (2-4) cm* (4-6) cm
Boron nitrogen composite powder is uniformly layered on carbon paper side by size, and laying width is 0.8cm-1.2cm, then with 5% nafion
It is dried after being fixed；Multiple (2-4) are cut into after carbon paper reverse side is equally uniformly layered on side with polyaniline, dries
The electrode of cm* (0.4-0.6) cm.
8. application of the boron-nitrogen compound in detection dopamine.
9. application according to claim 8, which is characterized in that the sample to be tested containing dopamine is uniformly blended in phosphorus
In phthalate buffer；Boron nitrogen composite powder is uniformly laid with and is fixed on carbon paper side, is uniformly laid in carbon paper reverse side
Polyaniline is used as working electrode after drying, electrochemical luminescence intensity detection is carried out using three-electrode system, by the electrochemistry of measurement
Luminous intensity is compareed with equation of linear regression, you can learn that the dopamine concentration in solution, and then obtain in sample to be tested
The content of dopamine.
10. application according to claim 9, which is characterized in that electrochemical luminescence intensity and dopamine concentration logarithm
(log) linear naturalization is carried out, corresponding equation of linear regression y (a.u.)=100.21x+6.74 (R is obtained2=0.99), y be to
The corresponding electrochemical luminescence intensity of sample, x be dopamine concentration logarithm (log), dopamine concentration range be 5.226 ×
10-4mol/L-0.4mol/L。
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