CN109620748B

CN109620748B - Anti-wrinkle essence and preparation method thereof

Info

Publication number: CN109620748B
Application number: CN201811590441.6A
Authority: CN
Inventors: 康立峰; 吴荣荣
Original assignee: Grf (wuhan) Biotechnology Inc
Current assignee: Grf (wuhan) Biotechnology Inc
Priority date: 2018-12-25
Filing date: 2018-12-25
Publication date: 2021-12-24
Anticipated expiration: 2038-12-25
Also published as: CN109620748A

Abstract

The invention discloses an anti-wrinkle essence and a preparation method thereof, wherein the anti-wrinkle essence comprises the following raw materials in percentage by mass: 5-15% of combined extract, 12-22% of active factor, 0.5-1.3% of propylene glycol, 0.3-1.2% of butanediol, 0.7-1.5% of diglycerol, 0.2-0.8% of acetylcysteine, 0.5-1.5% of betaine, 0.3-1.1% of carbomer, 0.6-2.3% of glycerol, 0.6-3.2% of ammonium acryloyldimethyl taurate/VP copolymer, 0.5-2.3% of hydroxyethyl cellulose, 0.4-3.2% of sodium hyaluronate, 0.3-2.1% of nicotinamide, 0.2-1.5% of panthenol, 1.2-8.5% of dipropylene glycol, 200.5-2.3% of polysorbate, 0.6-1.2% of disodium EDTA, 0.5-1.2% of dipotassium, 0.3-1.2% of 1, and the balance of deionized water, and the active factor and the combined extract are prepared into nano glycyrrhizic acid solution, the liquid crystal gel system is used for wrapping the nano solution, so that the nano solution is easier to absorb by a human body, meanwhile, the nano solution can be prevented from being oxidized, the slow release system of the liquid crystal gel is easier to promote the absorption by the human body, and the anti-wrinkle effect is enhanced.

Description

Anti-wrinkle essence and preparation method thereof

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to an anti-wrinkle essence and a preparation method thereof.

Background

The adolescence is the period of handsome and beautiful appearance in the whole life of a human, and at the moment, the skin is soft, smooth, glossy, full, elastic, white, transparent and red. By age 25, metabolism begins to slow, moisture and subcutaneous fat decrease, elastic fibers become thicker, and skin becomes wrinkled and lustrous to disappear. After age 40, the body hormone balance is disturbed, keratin is reduced, elastic fibers are broken, the skin is loose, rough and wrinkles are increased, and the skin gradually enters the aging stage. The aging of the skin is the prominent manifestation of the aging of the whole body, wherein the aging of the skin on the face is particularly obvious, the number of fibroblasts in the human body is gradually reduced, the collagen and the elastin which are secreted by the fibroblasts are also gradually reduced and broken by oxidation, the supporting effect on the skin is lost, the inhomogeneous collapse is caused, the dermis layer begins to be thinned, the elasticity of the skin is deteriorated, wrinkles also begin to be gradually generated, the wrinkles are one of the signs of the aging of the skin, the wrinkles are caused by the change of the supporting tissue and the skin structure of the skin after the human body is influenced by endogenous and exogenous factors, the water retention capacity of the skin is lost, the arrangement sequence of the elastin and the collagen is changed, the mechanical force and the elasticity of the skin are reduced, and the pits are formed, so that the wrinkles are formed.

The mechanism of wrinkle generation is complicated by cell damage caused by various factors including ultraviolet rays and apoptosis increased by the damage, hydrolysis of fibrous components such as collagen caused by increased expression of proteases such as Matrix Metalloproteinases (MMPs), and disruption of fiber bundles caused by increased expression of cytokines. Among them, MMPs play a critical role in this process, and have various functions such as degradation of extracellular matrix formed of collagen, proteoglycan, elastin, etc., and degradation of proteins expressed on the cell surface, etc. These degradations, in turn, lead to a reduction and degeneration of the extracellular matrix, which are important factors in the formation of wrinkles, sagging, etc. of the skin. In addition, inflammatory cytokines such as TNF- α, IL-1, and IL-6, which are produced by cells due to inflammation, can cause the formation of skin wrinkles and sagging by inducing the production of MMPs.

Skin aging is affected by various factors such as sunlight, environment, health, and the like, in addition to objective factors such as age, heredity, and the like. The skin is excessively exposed to the sun or the sun, so that the skin aging is accelerated, and pigment spots are easy to generate; the skin is damaged and the aging is accelerated when the skin is in a seriously polluted environment or excessively contacted with chemical substances harmful to the skin after long-term life; malnutrition, lassitude, entanglement of diseases and premature aging of the skin are more likely. Especially, the modern life is under heavy pressure, and the normal life is formed by staying overnight at work, long-time computer, smoking, insufficient sleep, long-time staying in an air-conditioned room, drinking, overuse of eyes and the like. According to the research of Dior Di Oromayan scientific center, every day, each layer of skin can accumulate a large amount of cell molecule toxin caused by internal or external factors, so that the cell asphyxia of the skin can be caused, and the toxin removing capability of the skin is influenced, thereby generating the troubles of dark skin color, lack of luster, full appearance of fatigue state and the like; the burden of the skin is increased, and the skin aging is accelerated.

Although skin aging is an irreversible physiological phenomenon, its progress can be slowed or slowed. For facial wrinkles, the common wrinkle-removing methods are surgical wrinkle-removing, injection wrinkle-removing and anti-wrinkle cosmetics. Surgical wrinkle-removing and injection wrinkle-removing treatments are costly and risky and not affordable to every consumer, and anti-wrinkle cosmetics are a good choice. The anti-wrinkle cosmetics are divided into anti-wrinkle facial masks, facial cleansers, anti-wrinkle toner, cream, essence and the like, and the anti-wrinkle facial masks and facial cleansers have short retention time on the face, unobvious effect, long-term use and inconvenient use; the anti-wrinkle toner and the anti-wrinkle cream have the advantages that the content of active ingredients in the anti-wrinkle toner and the anti-wrinkle cream is relatively low, the anti-wrinkle effect is not obvious, the nutrient substances and the active ingredients contained in the essence are more than those in the toner and the anti-wrinkle cream, the efficacy is strong, the anti-wrinkle effect is obvious, the anti-wrinkle products in the current market mainly aim at moisturizing and anti-free radicals, the functions are single, comprehensive factors generated by wrinkles cannot be considered, the effects are easily oxidized and lost, and the good anti-wrinkle effect cannot be achieved.

For example, patent with application number CN201510731589.7 discloses an essence with skin anti-wrinkle effect, which is characterized in that: the composition comprises the following components in parts by weight: the functional components are as follows: 0.01-20 parts of windmill grass extract, 0.01-20 parts of roselle flower extract, 0.001-10 parts of okra extract, 0.0001-10 parts of fullerene and 0.1-20 parts of small molecular hyaluronic acid; carrier: 0.1-10 parts of 1, 3-butanediol, 1-10 parts of glycerol, 0.05-0.5 part of phenoxyethanol and 60-90 parts of deionized water. The anti-wrinkle cream is compounded according to multiple mechanisms of skin wrinkle generation, and compounded components are innovated to obtain a formula combination with a synergistic effect.

Disclosure of Invention

The invention aims to provide an anti-wrinkle essence and a preparation method thereof, aiming at the problems in the prior art.

In order to achieve the purpose, the invention adopts the technical scheme that:

the anti-wrinkle essence comprises the following raw materials in percentage by mass: 5-15% of combined extracting solution, 12-22% of active factor, 0.5-1.3% of propylene glycol, 0.3-1.2% of butanediol, 0.7-1.5% of diglycerol, 0.2-0.8% of acetylcysteine, 0.5-1.5% of betaine, 0.3-1.1% of carbomer, 0.6-2.3% of glycerol, 0.6-3.2% of ammonium acryloyldimethyl taurate/VP copolymer, 0.5-2.3% of hydroxyethyl cellulose, 0.4-3.2% of sodium hyaluronate, 0.3-2.1% of nicotinamide, 0.2-1.5% of panthenol, 1.2-8.5% of dipropylene glycol, 200.5-2.3% of polysorbate-0.6-1.2% of disodium EDTA, 0.5-1.2% of dipotassium, 0.3-1.2% of 1, 2-hexanediol and the balance of deionized water.

Further, the combined extracting solution comprises centella asiatica extracting solution, chlorella extracting solution, glycyrrhiza glabra root extracting solution, selaginella tamariscina extracting solution and salix alba bark extracting solution which are added in equal amount.

Further, the preparation process of the centella extract, the chlorella extract, the glycyrrhiza glabra root extract, the selaginella extract and the white willow bark extract comprises the following steps: sterilizing herba Centellae, Chlorella, radix Glycytthizae, herba Selaginellae, and cortex Salicis Babylonicae with boiling water, cooling with cold water, cutting, adding into water, steaming for 1-2 hr, performing ultrasonic extraction at 12-16KHz and 21-31W, vacuum distilling at 60-80 deg.C, and concentrating.

Further, the active factors comprise acetyl hexapeptide-8, acetyl octapeptide-3, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and dipeptide diaminobutyrylbenzylamide diacetate which are added according to the mass percentage of 1:1:1:1: 1-5.

Further, the preparation method of the anti-wrinkle essence comprises the following steps:

(1) adding propylene glycol, butanediol, diglycerol, glycerol, panthenol, dipropylene glycol, 1, 2-hexanediol and polysorbate-20 into a water pot, heating to 100 ℃, uniformly stirring, cooling water to 70 ℃, adding sodium hyaluronate, disodium EDTA and dipotassium glycyrrhizinate while stirring, stirring until the sodium hyaluronate, disodium EDTA and dipotassium glycyrrhizinate are completely dissolved, continuously cooling to 40 ℃, adding acetylcysteine, betaine, carbomer, ammonium acryloyldimethyl taurate/VP copolymer, hydroxyethyl cellulose and nicotinamide, heating and melting, vortexing, fully and uniformly mixing, and standing at room temperature to obtain a liquid crystal gel solution;

(2) mixing the combined extracting solution and the active factors, adding the mixture into deionized water, uniformly stirring, performing ultrasonic dispersion, and performing high-pressure homogenization to obtain a nano solution;

(3) and (3) mixing the liquid crystal gel solution obtained in the step (1) and the nano solution obtained in the step (2), heating to 30 ℃, and slowly and uniformly stirring to obtain the anti-wrinkle essence.

Further, the power of ultrasonic dispersion in the step (2) is 750-.

Acetyl hexapeptide-8: participate in competing for the site of SNAP-25 on the vacuolar complex, thereby affecting complex formation. When the vesicular complex is slightly unstable, the vesicles cannot effectively release neurotransmitters, thereby causing a decrease in muscle contraction and preventing the formation of wrinkles.

Acetyl octapeptide-3: can effectively prevent excessive release of catecholamine and reduce wrinkles caused by facial expression muscle contraction.

Palmitoyl tripeptide-1: can promote the generation of collagen in a large amount, remarkably reduce facial wrinkles, enhance facial elasticity, promote cell activity, improve skin moisture content and improve skin color.

Palmitoyl tetrapeptide-7: the eye-care product has a strong drainage effect, can quickly drain excessive water, effectively eliminates edema and edema of the skin and the face, tightens the eye skin, accelerates the activation of skin cells around the eyes, accelerates the metabolism to fade dark circles, resists the looseness of the eye skin, and enables the skin around the eyes to be smooth, firm and elastic.

Dipeptide diaminobutyrylbenzylamide diacetate: can reduce the generation of wrinkles by inhibiting the contraction of muscles, and has excellent smoothing and quick wrinkle-removing properties.

Propylene glycol, butanediol, diglycerol, glycerol, panthenol, dipropylene glycol, 1, 2-hexanediol, polysorbate-20, sodium hyaluronate, disodium EDTA, dipotassium glycyrrhizinate, acetyl chitosamine, betaine, carbomer, ammonium acryloyldimethyl taurate/VP copolymer, hydroxyethyl cellulose and nicotinamide are heated, melted, swirled and fully mixed uniformly to prepare a liquid crystal gel solution, the combined extract and active factors are subjected to ultrasonic dispersion to prepare a nano solution, the liquid crystal gel solution is mixed with the nano solution to wrap the nano solution in the liquid crystal gel, the biocompatibility of the liquid crystal gel is good, the liquid crystal gel is convenient to absorb by a human body, and meanwhile, the nano solution is wrapped in the liquid crystal gel and is not easily oxidized by the outside, so that the effect is more durable; acetyl hexapeptide-8, acetyl octapeptide-3 and dipeptide diaminobutyrylbenzylamide diacetate inhibit and reduce the generation of muscle contraction and wrinkle, and meanwhile, dipeptide diaminobutyrylbenzylamide diacetate further dispels wrinkles and improves the smoothness of skin, so that the anti-wrinkle effect is better, palmitoyl tripeptide-1 promotes the generation of collagen, promotes the activity of cells and improves the water content of skin, palmitoyl tetrapeptide-7 quickly discharges excessive moisture, and the edema and edema of the skin and the face are eliminated, and the anti-wrinkle effect is greatly enhanced through the synergistic effect among the palmitoyl tetrapeptide-7.

The centella extract can promote blood circulation and collagen production, has a remarkable effect on after-sun repair after ultraviolet irradiation, can improve the contents of enzyme-type and non-enzyme-type antioxidant dismutase, hydrogen peroxide, glutathione peroxidase, vitamin C and ascorbic acid vitamin C in newly-produced tissues, and can inhibit and eliminate oxidative damage of free radicals to skin by bioactive substances contained in the centella extract; the chlorella extract has the functions of detoxifying, resisting oxidation and repairing damaged cells; the Glycyrrhiza glabra root extract can penetrate into the skin, keep high activity, prevent pachylosis and has anti-inflammatory and antibacterial effects; the herba Selaginellae extractive solution has effects of inhibiting inflammatory factor release, resisting inflammation, and resisting aging.

Compared with the prior art, the invention has the beneficial effects that:

1. acetyl hexapeptide-8, acetyl octapeptide-3 and dipeptide diaminobutyrylbenzylamide diacetate are used for inhibiting and reducing muscle contraction and wrinkle generation, palmitoyl tripeptide-1 promotes collagen generation, promotes cell activity and improves skin water content, palmitoyl tetrapeptide-7 quickly discharges excessive water to relieve edema and edema of the skin and the face, and the anti-wrinkle effect is greatly enhanced through the synergistic effect of the palmitoyl tripeptide-1 and the palmitoyl tetrapeptide-7.

2. The synergistic effect of the centella extract, the chlorella extract, the glycyrrhiza glabra root extract, the selaginella tamariscina extract and the white willow bark extract is used for supplementing collagen, eliminating and inhibiting the oxidative damage of free radicals to skin, and meanwhile, the effects of resisting inflammation and bacteria are achieved.

3. The combined extracting solution and the active factors are prepared into the nano solution, and the nano solution is wrapped by the liquid crystal gel, so that the liquid crystal gel has good biocompatibility, can promote the absorption of a human body, plays a role in slowly releasing the active factors, avoids the oxidation of the active factors by the outside to a certain extent, and enhances the anti-wrinkle effect.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The anti-wrinkle essence formulas of the examples 1 to 5 are shown in the table I:

table one: each group of test formula

The preparation method comprises the following steps:

(2) and mixing the combined extracting solution and the active factors, adding the mixed extracting solution and the active factors into deionized water, uniformly stirring, performing high-pressure homogenization after ultrasonic dispersion to obtain a nano solution, wherein the power of ultrasonic dispersion is 750-1000W, the ultrasonic time is 25-50min, the homogenization pressure is 800-1300bar, and the cycle time is 3-10 times.

Wherein: the preparation process of the centella extraction liquid, the chlorella extraction liquid, the glabrous greenbrier root extraction liquid, the selaginella extraction liquid and the white willow bark extraction liquid comprises the following steps: sterilizing herba Centellae, Chlorella, radix Glycytthizae, herba Selaginellae, and cortex Salicis Babylonicae with boiling water, cooling with cold water, cutting, adding into water, steaming for 1-2 hr, performing ultrasonic extraction at 12-16KHz and 21-31W, vacuum distilling at 60-80 deg.C, and concentrating.

Comparative example 1:

the difference from example 1 is that acetyl hexapeptide-8 and acetyl octapeptide-3 are not added, and other preparation conditions and methods are the same as example 1.

Comparative example 2:

the difference from example 1 is that palmitoyl tripeptide-1 is not added, and other preparation conditions and methods are the same as example 1.

Comparative example 3:

the difference from example 1 is that ultrasonic dispersion and high-pressure homogenization are not performed after the combined extract and the nanofactor are stirred and mixed, and other preparation conditions and methods are the same as example 1.

Comparative example 4:

the difference from example 1 is that the components of step (1) are merely heated and stirred to dissolve to form a mixed solution, and other preparation conditions and methods are the same as those of example 1.

And (3) efficacy verification:

the anti-wrinkle essence liquids prepared in examples 1 to 5 and comparative examples 1 to 5 were subjected to non-invasive human evaluation, and the test subjects: each group was 20 women (35-45 years old), test area: fishtail line area, use: twice a day for one month; testing indexes are as follows: skin smoothness, wrinkle depth, test results are shown in table two:

table two: test results

From the table results, it can be seen that acetyl hexapeptide-8, acetyl octapeptide-3 and dipeptide diaminobutyrylbenzylamide diacetate have a synergistic effect, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 have a synergistic effect, and the liquid crystal gel system has an anti-wrinkle effect better than that of an essence not adopting the liquid crystal gel system, and the essence prepared by preparing the active factors into the nano solution has an anti-wrinkle effect better than that of an essence not prepared into the nano solution.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The anti-wrinkle essence is characterized by comprising the following raw materials in percentage by mass: 5-15% of combined extracting solution, 12-22% of active factor, 0.5-1.3% of propylene glycol, 0.3-1.2% of butanediol, 0.7-1.5% of diglycerol, 0.2-0.8% of acetylcysteine, 0.5-1.5% of betaine, 0.3-1.1% of carbomer, 0.6-2.3% of glycerol, 0.6-3.2% of ammonium acryloyldimethyl taurate/VP copolymer, 0.5-2.3% of hydroxyethyl cellulose, 0.4-3.2% of sodium hyaluronate, 0.3-2.1% of nicotinamide, 0.2-1.5% of panthenol, 1.2-8.5% of dipropylene glycol, 200.5-2.3% of polysorbate, 0.6-1.2% of disodium EDTA, 0.5-1.2% of dipotassium, 0.3-1.2% of 1, 2-hexanediol, and the balance of deionized water;

the combined extracting solution is a centella asiatica extracting solution, a chlorella extracting solution, a glabrous glorybower root extracting solution, a selaginella tamariscina extracting solution and a white willow bark extracting solution which are added in equal amount;

the active factors are acetyl hexapeptide-8, acetyl octapeptide-3, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and dipeptide diaminobutyrylbenzylamide diacetate which are added according to the mass percentage of 1:1:1:1: 1-5;

the preparation method of the anti-wrinkle essence comprises the following steps:

2. The anti-wrinkle essence according to claim 1, wherein the preparation process of the centella asiatica extract, the chlorella vulgaris extract, the glycyrrhiza glabra root extract, the selaginella tamariscina extract and the salix alba bark extract comprises the following steps: sterilizing herba Centellae, Chlorella, radix Glycytthizae, herba Selaginellae, and cortex Salicis Babylonicae with boiling water, cooling with cold water, cutting, adding into water, steaming for 1-2 hr, performing ultrasonic extraction at 12-16KHz and 21-31W, vacuum distilling at 60-80 deg.C, and concentrating.

3. The anti-wrinkle essence as claimed in claim 1, wherein the ultrasonic dispersion power in step (2) is 750-.