CN109609346A - A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction - Google Patents
A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction Download PDFInfo
- Publication number
- CN109609346A CN109609346A CN201910114530.1A CN201910114530A CN109609346A CN 109609346 A CN109609346 A CN 109609346A CN 201910114530 A CN201910114530 A CN 201910114530A CN 109609346 A CN109609346 A CN 109609346A
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- Prior art keywords
- nucleic acid
- extraction
- extraction module
- magnetic bead
- module
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- 238000000605 extraction Methods 0.000 title claims abstract description 56
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000005298 paramagnetic effect Effects 0.000 title claims abstract description 8
- 239000002245 particle Substances 0.000 title claims abstract description 8
- 239000011324 bead Substances 0.000 claims abstract description 21
- 230000005291 magnetic effect Effects 0.000 claims abstract description 21
- 230000008859 change Effects 0.000 claims abstract description 10
- 238000010828 elution Methods 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims abstract description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention discloses a kind of method and apparatus based on paramagnetic particle method nucleic acid extraction, the method of the nucleic acid extraction including the following steps: (1) quick change head will be terminated on extraction module, casing is connect in extraction module lower end again, it is spare that the wireless charging module of extraction module is parked in charging at wireless charging；(2) it goes to take extraction module using mechanical arm；(3) casing is protruded into sample, opens the vibrating motor of extraction module, sample and magnetic bead are mixed, closed after adsorbing nucleic acid as far as possible with magnetic bead after a period of time, then open electromagnet, the magnetic bead with nucleic acid is adsorbed on sleeve surface；(4) extraction module is protruded into target tube, closes electromagnet, magnetic bead falls off, then opens vibrating motor, and magnetic bead and cleaning solution are mixed, and finally the extraction process entirely calculated is completed in elution.The equipment such as additional mechanical grip are eliminated, small volume and less weight saves the time of nucleic acid extraction, and efficiency greatly improves.
Description
Technical field
The present invention relates to nucleic acid extraction technical field, in particular to a kind of method based on paramagnetic particle method nucleic acid extraction and set
It is standby.
Background technique
The way of nucleic acid extraction before is mixed first is that being shaken microwell plate using oscillator, then recycles manipulator will
The microwell plate shaken is moved to bar magnet seat up, second is that the two requires additional mechanical grip, instrument using instrument for extracting nucleic acid
For device also than cumbersome, efficiency is not relatively high.
Summary of the invention
For above-mentioned deficiency in the prior art, the present invention provides a kind of method based on paramagnetic particle method nucleic acid extraction and set
Standby, the method for using supersonic oscillations mixes, and by pipette tips in conjunction with extraction module device, eliminates additional mechanical grip
Etc. equipment, small volume and less weight save the time of nucleic acid extraction, efficiency greatly improves.
In order to achieve the above object of the invention, the technical solution adopted by the present invention are as follows:
A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction, the equipment of the nucleic acid extraction include: quick change head, the quick change
Head connection mechanical arm and extraction module, the extraction module include: the wireless charging module of upper end, under the wireless charging module
Side's connection circuit board, circuit board lower section connecting vibration motor, the vibrating motor lower section connect electromagnet, the electromagnetism
Casing is arranged on the outside of iron；
The method of the nucleic acid extraction including the following steps:
(1) a quick change head will be terminated on extraction module, then casing is connect in extraction module lower end, by the wireless charging of extraction module
It is spare that electric module is parked in charging at wireless charging；
(2) it goes to take extraction module using mechanical arm；
(3) casing is protruded into sample, opens the vibrating motor of extraction module, sample and magnetic bead are mixed, made after a period of time
Nucleic acid is closed after adsorbing as far as possible with magnetic bead, then opens electromagnet, and the magnetic bead with nucleic acid is adsorbed on sleeve surface；
(4) extraction module is protruded into target tube, closes electromagnet, magnetic bead falls off, then opens vibrating motor, by magnetic bead and washing
Liquid mixes, and finally the extraction process entirely calculated is completed in elution.
The invention has the benefit that
The present invention is quickly mixed using supersonic oscillations；By pipette tips in conjunction with extraction module, remove the equipment such as additional machinery clamping jaw from；
Magnetic bead can be adsorbed on to disposable socket head surface using electromagnetic module, after energization, additional mechanical grip etc. is eliminated and set
Standby, small volume and less weight saves the time of nucleic acid extraction, and efficiency greatly improves.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
The appended drawing reference table of comparisons:
1- quick change head, 2- wiring board, 3- electromagnet, 4- wireless charging module, 5- vibrating motor, 6- casing, 8- extraction module.
Specific embodiment
Further illustrate a specific embodiment of the invention with reference to the accompanying drawing.Wherein identical components are with identical
Appended drawing reference indicates.
It should be noted that word "front", "rear" used in the following description, "left", "right", "up" and "down" refer to
Direction in attached drawing, word "inner" and "outside" refer respectively to the direction towards or away from geometric center of specific component.
In order that the present invention can be more clearly and readily understood, following will be combined with the drawings in the embodiments of the present invention,
Technical scheme in the embodiment of the invention is clearly and completely described.
As shown in Figure 1, a kind of method and apparatus based on paramagnetic particle method nucleic acid extraction, the equipment of the nucleic acid extraction include:
Quick change head 1, the quick change head 1 connects mechanical arm and extraction module 8, the extraction module 8 include: the wireless charging module of upper end
4, connect circuit board 2 below the wireless charging module 4, the described 2 lower section connecting vibration motor 5 of circuit board, the vibration is electric
Electromagnet 3 is connected below machine 5, is arranged casing 6 on the outside of the electromagnet 3；
The method of the nucleic acid extraction including the following steps:
(1) a quick change head 1 will be terminated on extraction module 8, then casing 6 is connect in 8 lower end of extraction module, by extraction module 8
It is spare that wireless charging module 4 is parked in charging at wireless charging；
(2) it goes to take extraction module 8 using mechanical arm；
(3) casing 6 is protruded into sample, opens the vibrating motor 5 of extraction module 8, sample and magnetic bead are mixed, after a period of time
It is closed after adsorbing nucleic acid as far as possible with magnetic bead, then opens electromagnet 3, the magnetic bead with nucleic acid is adsorbed on 6 surface of casing；
(4) extraction module 8 is protruded into target tube, close electromagnet 3, magnetic bead falls off, then opens vibrating motor 5, by magnetic bead with
Cleaning solution mixes, and finally the extraction process entirely calculated is completed in elution.
The above description is only a preferred embodiment of the patent of the present invention, is not intended to limit the invention patent, all at this
Made any modifications, equivalent replacements, and improvements etc., should be included in the invention patent within the spirit and principle of patent of invention
Protection scope within.
Claims (1)
1. a kind of method and apparatus based on paramagnetic particle method nucleic acid extraction, which is characterized in that the equipment of the nucleic acid extraction includes: fast
It changes head (1), quick change head (1) the connection mechanical arm and extraction module (8), the extraction module (8) include: the wireless charging of upper end
Electric module (4), wireless charging module (4) lower section connect circuit board (2), and connection vibration is electric below the described circuit board (2)
Machine (5), vibrating motor (5) lower section connect electromagnet (3), are arranged casing (6) on the outside of the electromagnet (3)；
The method of the nucleic acid extraction including the following steps:
(1) a quick change head (1) will be terminated on extraction module (8), then casing (6) is connect in extraction module (8) lower end, will extract
It is spare that the wireless charging module (4) of module (8) is parked in charging at wireless charging；
(2) it goes to take extraction module (8) using mechanical arm；
(3) casing (6) is protruded into sample, opens the vibrating motor (5) of extraction module (8), sample and magnetic bead mixed, one section
It is closed after adsorbing nucleic acid as far as possible with magnetic bead, then opens electromagnet (3), the magnetic bead with nucleic acid is adsorbed on set
Manage (6) surface；
(4) extraction module (8) is protruded into target tube, is closed electromagnet (3), magnetic bead falls off, then opens vibrating motor (5), will
Magnetic bead and cleaning solution mix, and finally the extraction process entirely calculated is completed in elution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910114530.1A CN109609346A (en) | 2019-02-14 | 2019-02-14 | A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910114530.1A CN109609346A (en) | 2019-02-14 | 2019-02-14 | A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109609346A true CN109609346A (en) | 2019-04-12 |
Family
ID=66019734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910114530.1A Pending CN109609346A (en) | 2019-02-14 | 2019-02-14 | A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109609346A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN205635637U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing subassembly that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
| CN205635571U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
-
2019
- 2019-02-14 CN CN201910114530.1A patent/CN109609346A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN205635637U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing subassembly that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
| CN205635571U (en) * | 2016-05-24 | 2016-10-12 | 苏州英芮诚生化科技有限公司 | A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190412 |