CN109588717A - A kind of method preparing class wild ginseng product and class wild ginseng product - Google Patents
A kind of method preparing class wild ginseng product and class wild ginseng product Download PDFInfo
- Publication number
- CN109588717A CN109588717A CN201811310182.7A CN201811310182A CN109588717A CN 109588717 A CN109588717 A CN 109588717A CN 201811310182 A CN201811310182 A CN 201811310182A CN 109588717 A CN109588717 A CN 109588717A
- Authority
- CN
- China
- Prior art keywords
- ginseng
- wild ginseng
- wild
- class
- ginsenoside
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 286
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 286
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 286
- 238000000034 method Methods 0.000 title claims abstract description 17
- 229930182494 ginsenoside Natural products 0.000 claims description 57
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- 239000000047 product Substances 0.000 claims description 44
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- FBFMBWCLBGQEBU-RXMALORBSA-N (2s,3r,4s,5s,6r)-2-[(2r,3r,4s,5s,6r)-2-[[(3s,5r,6s,8r,9r,10r,12r,13r,14r,17s)-3,12-dihydroxy-4,4,8,10,14-pentamethyl-17-[(2s)-6-methyl-2-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhept-5-en-2-yl]-2,3,5,6,7,9,11,12,13,15,16,17-dodecah Chemical compound O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O FBFMBWCLBGQEBU-RXMALORBSA-N 0.000 description 3
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- JDCPEKQWFDWQLI-LUQKBWBOSA-N ginsenoside Rc Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1O[C@@H](CO)[C@H](O)[C@H]1O JDCPEKQWFDWQLI-LUQKBWBOSA-N 0.000 description 3
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- RAQNTCRNSXYLAH-UHFFFAOYSA-N Ginsenoside Rh1 Natural products CC(C)=CCCC(C)(O)C1CCC(C2(C3)C)(C)C1C(O)CC2C1(C)CCC(O)C(C)(C)C1C3OC1OC(CO)C(O)C(O)C1O RAQNTCRNSXYLAH-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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Abstract
The invention discloses a kind of method for preparing class wild ginseng and class wild ginseng products, purpose is to provide a kind of method for preparing class wild ginseng by biotechnology and chemical analysis technology for ginseng market, and class wild ginseng product is prepared by this method, be based primarily upon wild ginseng, Ginseng under Forest, garden ginsent finger-print type and concentration difference, by between the proportion major biochemical ingredient, accordingly it is concentrated or is diluted, to deploy, be combined into class wild ginseng product, the finger-print of such wild ginseng product is all similar with wild ginseng in type and abundance.Therefore, the preparation method is practical, reliable, and the class wild ginseng product prepared and wild ginseng have same health care and medicinal value, can effectively solve the problems, such as that wild ginseng is rare and be unable to satisfy people's use demand in the market.
Description
[technical field]
The present invention relates to field of health care products more particularly to a kind of methods for preparing class wild ginseng product and class wild ginseng to produce
Product.
[technical background]
NATURAL DISTRIBUTION, naturally procreation, naturally ginseng of the growth more than or equal to 15 years, referred to as wild in original remote, thickly forested mountains
Wild ginseng.Wild ginseng is the superfine product in ginseng, and with the growth in ginseng age, the type of ginsenoside is more, and abundance is higher, the total soap of ginseng
The content of glycosides is also higher.Wild ginseng have reinforce vital energy, macrobiosis of making light of one's life by commiting suicide, invigorating the spleen of promoting the production of body fluid, tranquilize the mind and promote the intelligence and other effects, as health care product
It is edible to emphasize necessary long term usage, the conditioning product of organism balance and normal condition are to maintain, are the rare medicinal herbs having won fame both at home and abroad.
However, since the growing environment of wild ginseng requires harshness, and the growth year that it is needed is long, various bioids
It studies and point is superior to other ginseng kinds in terms of type and content, and pharmacological action is also more significant than Ginseng under Forest and garden ginsent, therefore
In ginseng, supply falls short of demand in the market all the time, expensive.Detailed investigation of related literatures and some experimental data based on early period, I
Find, ginsenoside, amino acid, inorganic elements, the contents of saccharide of garden ginsent are not only below wild ginseng, and Ginseng under Forest, garden ginsent
Lack the unique rare ginsenoside ingredient contained in certain wild ginsengs.This is to cause wild ginseng and Ginseng under Forest, garden ginsent in biology
One of different reasons on activity intensity.Ginseng Products refer to using ginseng or ginseng extract as primary raw material there is nourishing or special
The product for determining healthcare function belongs to Chinese herbal medicine, tradition nourishing class health care product major class.For the quality for improving Ginseng Products, can use
The ingredient of wild ginseng is raw material, but not only cost is very high for such scheme, it is also difficult to sustainable supply.
For above-mentioned realistic problem, the present patent application is proposed hereby.
[summary of the invention]
A kind of method for preparing class wild ginseng of the present invention and class wild ginseng product, it is therefore intended that be by biotechnological method
Ginseng market provides a kind of method for preparing class wild ginseng, and the class wild ginseng product prepared by this method, is based primarily upon
Wild ginseng, Ginseng under Forest, garden ginsent finger-print type and concentration difference, by between matching major biochemical ingredient
Than being accordingly concentrated or being diluted, to deploy, be combined into class wild ginseng product, the biochemical indicator of such wild ginseng product is being planted
It is all similar with wild ginseng in class and abundance；Therefore, the preparation method is practical, reliable, and the class wild ginseng product prepared with
Wild ginseng have same health care and medicinal value, can effectively solve wild ginseng in the market it is rare and be unable to satisfy people using need
The problem of asking.
A kind of method for preparing class wild ginseng product of the present invention, includes the following steps:
A, by Ginseng under Forest or garden ginsent extraction separation and purification, ginsenoside is obtained；Or it is obtained using genetic engineering enzymatic conversion
Ginsenoside；
B, referring to the proportion between saponin constituent in wild ginseng finger-print, phase is carried out to step A ginsenoside obtained
The concentration or dilution answered obtain class wild ginseng saponin(e to deploy, be combined into saponin constituent identical with wild ginseng；
C, the extraction separation and purification from Ginseng under Forest or garden ginsent, obtains the carbohydrate of ginseng；
D, referring to the proportion of carbohydrate in wild ginseng finger-print, carbohydrate obtained by step C is accordingly concentrated or is diluted, to adjust
Match, be combined into carbohydrate content identical with wild ginseng, obtains class wild ginseng carbohydrate；
E, referring to the proportion of saponin constituent and carbohydrate content in wild ginseng finger-print, by the resulting class wild ginseng of step B
Saponin(e concentration corresponding to the resulting class wild ginseng carbohydrate of D step or dilution, to deploy, be combined into proportion identical with wild ginseng,
Obtain semi-finished product a；
F, referring to the ratio of various amino acid in wild ginseng finger-print, various amino acid are accordingly added in semi-finished product a,
To deploy, be combined into the amino acid classes and proportional amount of proportion identical with wild ginseng, semi-finished product b is obtained；
G, referring to the ratio of each microelement in wild ginseng finger-print, various inorganic members are accordingly added in semi-finished product b
Element obtains semi-finished product c to deploy, be combined into inorganic elements proportion identical with wild ginseng；
H, referring to the proportion between wild ginseng finger-print Biochemical Components, semi-finished product c is accordingly concentrated to or is diluted allotment, group
It closes, obtains class wild ginseng product.
The present invention also provides a type wild ginseng product, the method for using above-mentioned preparation class wild ginseng product.
Compared with prior art, the present invention has the advantage that
1, Ginseng under Forest big using usage amount and more economical price, garden ginsent be as the raw material for producing class wild ginseng product, from
Middle extraction general ginsenoside and isolate and purify and wild ginseng comparison the higher ginsenoside of content, or using genetic recombination enzyme turn
Change the biotechnological methods such as isolates and purifies and obtains various ginsenosides, and ginseng part carbohydrate is by extracting separation concentration or dilute
It releases, microelement, amino acid are added by proper ratio external source, are concentrated eventually by the matching method between each ingredient or dilution is adjusted
With being combined into class wild ginseng product, thus make the biochemical composition of class Ginseng Products of the invention in type and abundance all with
Wild ginseng is similar.
2, in this way, can effectively solve the problems, such as that for many years wild ginseng is short and irreplaceable.
[Detailed description of the invention]
Fig. 1 be wild ginseng, Ginseng under Forest, garden ginsent different times general ginsenoside comparison schematic diagram.
Fig. 2 is the comparison schematic diagram of 8 kinds of saponin(es in the wild ginseng in different ginseng ages.
Fig. 3 is 8 kinds of content of ginsenoside comparison schematic diagrams of 15 years wild ginsengs and Ginseng under Forest, 6 years garden ginsents.
Fig. 4 is 8 kinds of content of ginsenoside comparison schematic diagrams of 25 years wild ginsengs and Ginseng under Forest, 6 years garden ginsents.
Fig. 5 is 8 kinds of content of ginsenoside comparison schematic diagrams of 40 years wild ginsengs and Ginseng under Forest, 6 years garden ginsents.
Fig. 6 is the comparison schematic diagram of wild ginseng, Ginseng under Forest, 6 years garden ginsent carbohydrates.
Fig. 7 is the amino acid content comparison schematic diagram of 20 years wild ginsengs, Ginseng under Forest, 6 years garden ginsents.
Fig. 8 is the amino acid content comparison schematic diagram of 30 years wild ginsengs, Ginseng under Forest, 6 years garden ginsents.
Fig. 9 is the inorganic element content comparison schematic diagram of wild ginseng, Ginseng under Forest, 6 years garden ginsents.
[specific embodiment]
Wild ginseng have reinforce vital energy, macrobiosis of making light of one's life by commiting suicide, invigorating the spleen of promoting the production of body fluid, tranquilize the mind and promote the intelligence and other effects.Wild ginseng refers to unartificial
Sowing is grown on ginseng of the ginseng age more than or equal to 15 years under natural conditions.Ginseng under Forest refers to the kind of hayashishita artificial seeding's Cultivated shen
Son grows the ginseng of 10-20 or more naturally.Garden ginsent, which refers to, to be grown in artificial culture environment, and ginseng age is more than or equal to 5 years,
And the ginseng less than or equal to 10 years.Existing research shows that the effect of wild ginseng is more significant than garden ginsent, and Ginseng under Forest is then between open country
Wild ginseng and garden ginsent are between the two.Currently, biological medicine it is big health in the market, wild ginseng is to belong to rare medicinal raw material.Needle
To on existing market wild ginseng Related product it is rare, goal of the invention is to provide a kind of class wild ginseng product for preparing for market
Method and class wild ginseng product, mainly include from Ginseng under Forest or garden ginsent extraction separation and purification obtain various ginsenosides, carbohydrate,
Amino acid, microelement etc. refer again to the wild ginseng finger-print of various years, pass through the proportion to each ingredient, concentration or dilute
It releases to deploy, be combined into class wild ginseng product.Ginsenoside, amino acid, microelement, carbohydrate, volatilization in class wild ginseng product
The finger-prints such as oil, vitamin are all similar with wild ginseng in type and content, therefore such product is referred to as class wild ginseng product.
Ginseng under Forest, garden ginsent ginsenoside ingredient extraction separation and purification:
1. the selection standard of Ginseng under Forest: using the Ginseng under Forest with medical value as raw material, best Ginseng under Forest ginseng age is
10 years or more.
It takes ginseng that must clean, shreds, hot water is added and extracts about 3 hours, filtrate is collected by filtration, filter residue is in this high temperature
Boiling is extracted 2 hours, and filtrate is collected by filtration, and is merged filtrate twice, is obtained ginsenoside total filtrate, polishing purification isolates phase
The ginsenoside high for content in wild ginseng, the higher saponin(e of total ginsenoside and content being concentrated and separated.
2. the selection standard of garden ginsent: using 6 years or more garden ginsents as raw material, taking ginseng that must clean, shred, add distillation
Water thermophilic digestion extracts 3 hours, and filtrate is collected in filtering；Filter residue adds distilled water thermophilic digestion to extract 2 hours again, filters, and collects
Filtrate, merging extract feed liquid twice, obtain ginsenoside total filtrate, further polishing purification is isolated relative in wild ginseng
The high ginsenoside of content, the higher saponin(e of total ginsenoside and content being concentrated and separated.It is finally concentrated under vacuum dry
It is dry, ginsenoside extract is obtained, broken, sieving is made.
Genetic engineering enzymatic conversion is used to isolate and purify to obtain main ginsenoside and rare ginsenoside:
Clone obtains beta-galactosidase gene recombinant conversion and expresses into saccharomyces cerevisiae, isolates and purifies recombination beta galactose
Glycosides enzyme, the conversion condition of optimum combination beta galactosidase, under recombination beta galactosidase effect, ginsenoside Re is converted into
Ginsenoside Rg1 and Rb1；Clone's beta-glucosidase gene, which is transformed into saccharomyces cerevisiae, expresses, and isolates and purifies recombinant beta-glucose
Glycosides enzyme, the conversion condition of optimum combination beta-glucosidase, recombination beta-glucosidase effect under, ginsenoside Re, Rg1,
Rb1 is converted into rare ginsenoside Rh1, Rh2, Rg2.Be finally separating purifying, be concentrated and dried, obtain ginsenoside monomer Rg1,
Rb1,Rh1,Rh2,Rg2.The saponin(e that the present embodiment 2 obtains is for adding the less ginsenoside of content, such as ginsenoside Rb1.
The extraction separation and purification of Ginseng under Forest and garden ginsent ginseng carbohydrate content:
1. the selection standard of Ginseng under Forest: using the Ginseng under Forest with medical value as raw material.
Go ginseng that must clean, chopping grinding takes dry ginseng powder, adds distilled water, extracts 3- in 100 DEG C of water-baths
4h, filtering.Filter residue adds 20-30 times to measure water extraction again, extracts 3 times, merging filtrate, and filtrate decompression concentration adds 95% ethyl alcohol to be adjusted to
Determining alcohol is 75%, and alcohol precipitation is overnight, is filtered, and precipitating is successively washed with dehydrated alcohol, acetone, ether, dry, obtains Thick many candies.With
TCA method obtains polysaccharide extraction liquid except deproteinized, then isolates and purifies polysaccharide through sephadex chromatography.
2. the selection standard of garden ginsent: using 6 years or more garden ginsents as raw material.
Go ginseng that must clean, chopping grinding takes dry ginseng powder, adds distilled water, extracts 3- in 100 DEG C of water-baths
4h, filtering.Filter residue adds 20-30 times to measure water extraction again, extracts 3 times, merging filtrate, and filtrate decompression concentration adds 95% ethyl alcohol to be adjusted to
Determining alcohol is 75%, and alcohol precipitation is overnight, is filtered, and precipitating is successively washed with dehydrated alcohol, acetone, ether, dry, obtains Thick many candies.With
TCA method obtains polysaccharide extraction liquid except deproteinized, then isolates and purifies polysaccharide through sephadex chromatography.
Summary and induction goes out ginsenoside type and content finger-print in the wild ginseng of different years:
According to the type and content analysis of ginsenoside in existing wild ginseng, summary and induction goes out the wild ginseng of different years
Middle ginsenoside type and content finger-print, and carried out with ginsenoside type and content finger-print in Ginseng under Forest, garden ginsent
Reference is realized main to obtain the concentration for producing class wild ginseng based on Ginseng under Forest, garden ginsent or diluted allotment coefficient
Some Circulating Factors-ginsenoside class wild ginseng in type and content industrialized production.General ginsenoside in wild ginseng contains
Amount is higher than the total Ginsenosides Content in garden ginsent, is number of the different times to total Ginsenosides Content as shown in the following table 1 and Fig. 1
Result according to statistics.
The comparison (unit: %) of 1 different times general ginsenoside of table
Wild ginseng grows into 15 years, ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rd, Rg2 the total peace treaty of content
3.64%, grow into 25 years this 8 kinds of ginsenosides content always and about 4.22%, about 4.99% when growth 40 years.Thus
It is found that the ginsenoside in wild ginseng increases with the growth in ginseng age.8 kinds of ginsenosides detailed description are as follows table 2 and Fig. 2
It is shown.
The comparison (unit: %) of 8 kinds of saponin(es in the different ginseng age wild ginsengs of table 2
As shown in table 3 and figure 3, it is analyzed according to existing data, existing more than 50 kinds of ginsenoside structure are elucidated with, wherein
In wild ginseng and garden ginsent content it is higher have main saponin(e ginsenosides Rg1, Re and Rb1 and rare saponin(e Rf, Rc, Rb2, Rd,
The comparision contents of Rg2, other ginsenosides are few, or even the saponin(e having is close to trace.In the ginsenoside of wild ginseng and garden ginsent
In comparing, the general ginsenoside of wild ginseng is greater than Ginseng under Forest, 1-3 times of general ginsenoside of garden ginsent.In 15 years wild ginsengs and 6 years
In garden ginsent, wild ginseng Ginsenoside Rc is garden ginsent more than 4 times, and wild ginseng ginsenoside Rb1 is garden ginsent more than 2 times, wild ginseng people
Ginseng saponin(e Re is 3 times of Ginseng under Forest or so, is 1 times of garden ginsent or so, and the ginsenoside Rg1 of wild ginseng is lower than Ginseng under Forest and garden
Ginseng, ginsenoside Rf is lower than Ginseng under Forest, garden ginsent in wild ginseng, the content of the ginsenoside Rb2 of wild ginseng and ginsenoside Rd with
Garden ginsent but is below about 1-3 times or so of content of Ginseng under Forest without significant difference, and wild ginseng kind Rg2 is about 2 times of garden ginsent.
The various content of ginsenoside ratios of wild ginseng according to compiling above and by verification are subtracted or are added, and mixing is equal
Even, allotment obtains 15 years wild ginseng ginsenoside products of class.
3 15 years wild ginsengs of table, Ginseng under Forest, 6 years garden ginsents 8 kinds of content of ginsenoside compare (unit %)
As shown in table 4 and figure 4, it is analyzed according to existing data, existing more than 50 kinds of ginsenoside structure are elucidated with, wherein
In wild ginseng and Ginseng under Forest, garden ginsent content it is higher have main ginsenosides Rg1, Re and Rb1 and rare ginsenoside Rf, Rc,
The comparision contents of Rb2, Rd, Rg2, other ginsenosides are few, or even the saponin(e having is close to trace.In 25 years wild ginsengs and 6 years
In garden ginsent, wild ginseng Ginsenoside Rc is almost 6 times of garden ginsent, but is lower than the content of Ginseng under Forest Rc；Wild ginseng ginseng
Saponin(e Rb1 is 2.5 times of Ginseng under Forest and garden ginsent；Wild ginseng ginsenoside Re is approximately 3 times of Ginseng under Forest, is garden ginsent more than 1 times；It is wild
Wild ginseng ginsenoside Rg2 is approximately 2 times of garden ginsent, but differs relatively small with Ginseng under Forest comparison；The ginsenoside Rg1 of wild ginseng is lower than
Garden ginsent and 1~2 times of Ginseng under Forest；Ginsenoside Rf, Rb2, Rd of wild ginseng are significantly lower than Ginseng under Forest, the ginsenoside of wild ginseng
Rf, ginsenoside Rb2, the content of ginsenoside Rd and garden ginsent are without significant difference.It is verified according to compiling and passing through above
The various content of ginsenoside ratios of wild ginseng reduce or addition, be uniformly mixed, allotment obtains 25 years wild ginseng ginseng soaps of class
Glycoside product.
4 25 years wild ginsengs of table and Ginseng under Forest, 6 years garden ginsents 8 kinds of content of ginsenoside compared with (unit %)
As shown in table 5 and Fig. 5, to be analyzed according to existing data, existing more than 50 kinds of ginsenoside structure are elucidated with, wherein
Content is higher in wild ginseng and garden ginsent ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rd, Rg2, other ginsenosides
Comparision contents are few, or even the saponin(e having is close to trace.In 40 years wild ginsengs and 6 years garden ginsents, wild ginseng Ginsenoside Rc is
More than 4 times of garden ginsent, Ginseng under Forest is more slightly higher than wild ginseng；Wild ginseng ginsenoside Rb1 is 3.5-5 times of Ginseng under Forest and garden ginsent,
Wild ginseng ginsenoside Re is garden ginsent and more than 1-2 times of Ginseng under Forest, and ginsenoside Rd is lower than Ginseng under Forest in wild ginseng；Wild ginseng
Ginsenoside Rg1 is lower than Ginseng under Forest, garden ginsent；Ginsenoside Rf is lower than Ginseng under Forest in wild ginseng, and the content of ginsenoside Rb2 is out of office
Wild ginseng and garden ginsent are between the two without significant difference, but Ginseng under Forest is higher than wild ginseng.It compiles and according to above by core
The various content of ginsenoside ratios of real wild ginseng reduce or addition, are uniformly mixed, and allotment obtains 40 years wild ginseng ginsengs of class
Saponin(e product.
5 40 years wild ginsengs of table and Ginseng under Forest, 6 years garden ginsents main content of ginsenoside compared with (unit %)
It deploys, be combined into carbohydrate content identical with wild ginseng:
Wild ginseng, Ginseng under Forest, carbohydrate accounts in garden ginsent ratio are more, mainly there is reduction sugar-type polysaccharide and soluble polysaccharide,
Using concentration or dilution allotment.It is about 43.87~49.55% in the range of wild ginseng kind total sugar content, the total reducing sugar of Ginseng under Forest contains
Amount about 45.21~48.70%, therefore wild ginseng kind total sugar content is close compared with the total sugar content of Ginseng under Forest, wild ginseng kind is total
Sugared content differs bigger with the total sugar content of garden ginsent, and the total sugar content of garden ginsent is 54.29~57.39%.It is more for reproducibility
Sugared content, wild ginseng is close with Ginseng under Forest, but more much lower than garden ginsent, wherein wild ginseng reductive polysacchride content: 3.36~
4.68%, garden ginsent reductive polysacchride content: 13.21~14.31%.For soluble polysaccharide content, wild ginseng and Ginseng under Forest ratio
It is more close, but wild ginseng differs larger with garden ginsent, wherein wild ginseng soluble polysaccharide content: 12.04~13.74%, garden
Ginseng soluble polysaccharide content: 6.50~8.02%.The contents of saccharide of three kinds of ginsengs compares as shown in table 6 and Fig. 6.
The comparison (unit: %) of 6 wild ginseng of table, Ginseng under Forest, garden ginsent carbohydrate
Deploy, be combined into the amino acid classes and proportional amount of proportion identical with wild ginseng:
According to the type and content analysis of amino acid in existing wild ginseng, summary and induction goes out in the wild ginseng of different years
Amino acid classes and content, and carry out reference with amino acid classes in Ginseng under Forest, garden ginsent and content, thus obtain with Ginseng under Forest,
Concentration or the dilution allotment coefficient that class wild ginseng amino acid is produced based on garden ginsent, realize class wild ginseng product amino acid in type
And the industrialized production in content.
By amino acid content and concentrations versus, poor amino acid, is eaten using amino acid in Ginseng under Forest and garden ginsent
The addition of product additive, make amino acid in class Ginseng Products reaches the amino acid content being similar in wild ginseng containing ratio.Join age
For 20 years wild ginsengs compared with amino acid content between Ginseng under Forest, 6 years garden ginsents as shown in table 7 and Fig. 7.
7 20 years wild ginsengs of table, Ginseng under Forest, 6 years garden ginsents amino acid content compare (unit: %)
Join the comparision contents of amino acid between the age wild ginseng for being 30 years and Ginseng under Forest, 6 years garden ginsents as shown in table 8 and Fig. 8.
8 30 years wild ginsengs of table, Ginseng under Forest, 6 years garden ginsents amino acid content compare (unit: %)
It deploys, be combined into inorganic elements proportion identical with wild ginseng:
According to the type and content analysis of inorganic elements in existing wild ginseng, summary and induction goes out the wild ginseng of different years
Middle inorganic elements type and content, and reference is carried out with inorganic elements type and content in Ginseng under Forest, garden ginsent, to obtain with woods
Concentration or the dilution allotment coefficient that class wild ginseng is produced based on lower ginseng, garden ginsent, realize class wild ginseng product in inorganic elements side
The industrialized production in face.Heavy metal element Hg, As, Cd, Pd content in wild ginseng is almost trace, and most is institute in garden ginsent
Contain, but because they have great harm to human body, should not be added in class wild ginseng product.These element majorities be because
For environment pollution and bring.
By content balance, poor inorganic elements in Ginseng under Forest and garden ginsent uses inorganic elements food additives
Addition, makes the content ratio of inorganic elements in class Ginseng Products reach the inorganic element content being similar in wild ginseng.Inorganic member
The content balance of element is as shown in table 9 and Fig. 9.
9 wild ginseng of table, Ginseng under Forest, garden ginsent inorganic element content compare (unit: %)
| Inorganic elements | Wild ginseng | Ginseng under Forest | Garden ginsent |
| K | 1.15±0.15 | 1.01±0.16 | 0.97±0.25 |
| Ca | 6.24×10-1±2.7×10-1 | 8.22×10-1±1.55×10-1 | 6.84×10-1±2.04×10-1 |
| Mg | 1.70×10-1±4.6×10-2 | 1.78×10-1±8.1×10-2 | 7.52×10-1±1.52×10-1 |
| P | 7.80×10-2±3×10-2 | 1.44×10-1±4.1×10-2 | 2.00×10-1±8.7×10-2 |
| Al | 4.50×10-2±1.7×10-2 | 1.80×10-2±4×10-3 | 1.70×10-2±7×10-3 |
| Fe | 5.00×10-2±1.1×10-2 | 1.50×10-2±4×10-3 | 1.50×10-2±8×10-3 |
| Na | 3.20×10-2±3×10-3 | 2.30×10-2±5×10-3 | 2.20×10-2±6×10-3 |
| Mn | 5.00×10-3±4×10-3 | 1.10×10-2±4×10-3 | 4.00×10-3±2×10-3 |
| Zn | 5.00×10-3±3×10-3 | 4×10-3±1×10-3 | 2.00×10-3±9×10-4 |
| Cu | 5.00×10-4±3×10-4 | 1.70×10-3±5×10-4 | 2.00×10-3±×10-4 |
| Pd | 2.00×10-5±1.8×10-5 | 8.30×10-4±3.3×10-4 | 2.00×10-4±1×10-4 |
| Ge | 5.90×10-5±2.1×10-5 | 5.80×10-5±3.1×10-5 | 2.37×10-4±1.43×10-4 |
| As | 9.00×10-6±4.5×10-6 | 6.50×10-6±2.5×10-6 | 5.80×10-5±2.6×10-5 |
| Cd | 1.70×10-5±5×10-6 | 5.00×10-6±3×10-6 | 3.20×10-5±1.2×10-5 |
| Hg | 1.00×10-6±5×10-7 | 3.50×10-6±3.5×10-6 | 7.00×10-6±5×10-6 |
Claims (2)
1. a kind of method for preparing class wild ginseng product, it is characterised in that include the following steps:
A, by Ginseng under Forest or garden ginsent extraction separation and purification, ginsenoside is obtained；Or ginseng is obtained using genetic engineering enzymatic conversion
Saponin(e；
B, referring to the proportion between saponin constituent in wild ginseng finger-print, step A ginsenoside obtained is carried out corresponding
Concentration or dilution, to deploy, be combined into saponin constituent identical with wild ginseng, obtain class wild ginseng saponin(e；
C, the extraction separation and purification from Ginseng under Forest or garden ginsent, obtains the carbohydrate of ginseng；
D, referring to the proportion of carbohydrate in wild ginseng finger-print, carbohydrate obtained by step C is accordingly concentrated or is diluted, with allotment, group
Carbohydrate content identical with wild ginseng is closed out, class wild ginseng carbohydrate is obtained；
E, referring to the proportion of saponin constituent and carbohydrate content in wild ginseng finger-print, by the resulting class wild ginseng saponin(e of step B
Concentration corresponding to the resulting class wild ginseng carbohydrate of D step or dilution, to deploy, be combined into proportion identical with wild ginseng, obtain
Semi-finished product a；
F, referring to the ratio of various amino acid in wild ginseng finger-print, various amino acid are accordingly added in semi-finished product a, to adjust
The amino acid classes and proportional amount for matching, being combined into proportion identical with wild ginseng, obtain semi-finished product b；
G, referring to the ratio of each inorganic elements in wild ginseng finger-print, various inorganic elements are accordingly added in semi-finished product b, with
It deploys, be combined into inorganic elements proportion identical with wild ginseng, obtain semi-finished product c；
H, referring to the proportion between wild ginseng finger-print Biochemical Components, semi-finished product c is accordingly concentrated to or is diluted allotment, combination,
Obtain class wild ginseng product.
2. a type wild ginseng product, it is characterised in that using the method for preparing class wild ginseng product described in claim 1.
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| CN118576629A (en) * | 2024-05-22 | 2024-09-03 | 广东参宫生命科技有限公司 | A kind of enzymatic hydrolysis and extraction equipment and method of wild ginseng-like products |
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| CN118576629A (en) * | 2024-05-22 | 2024-09-03 | 广东参宫生命科技有限公司 | A kind of enzymatic hydrolysis and extraction equipment and method of wild ginseng-like products |
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