CN109580314A - A kind of the coverslip pick device and its application method of immunohistochemical staining machine - Google Patents

A kind of the coverslip pick device and its application method of immunohistochemical staining machine Download PDF

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Publication number
CN109580314A
CN109580314A CN201811258481.0A CN201811258481A CN109580314A CN 109580314 A CN109580314 A CN 109580314A CN 201811258481 A CN201811258481 A CN 201811258481A CN 109580314 A CN109580314 A CN 109580314A
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cover glass
coupling
head
seat
suction cup
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CN109580314B (en
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刘军
刘钢成
唐第霞
刘叶静
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Urit Medical Electronic Co Ltd
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Urit Medical Electronic Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
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Abstract

The present invention a kind of the coverslip pick device and its application method of immunohistochemical staining machine, the present apparatus is equipped with coupling arrangement in the work head of immunohistochemical staining machine, and main body is the component for having hollow cavity, and upper end is gas-tpe fitting, transtracheal connection injection air pump, lower end is the coupling head of cone.Coverslip pick tool main body is connection seat, and connection seat upper section lumen is conical lumen identical with coupling head, and bottom end is silicon rubber suction cup.Coupling head is inserted into connection seat, and the two surface is bonded to each other interference fit, and the lumen seamless connection of the two.Its application method is after coupling head is inserted into the connection of both connection seats by work head, to be moved to above coverslip, so that silicon rubber suction cup is generated negative pressure by air pump, adsorbs coverslip, silicon rubber suction cup generates positive pressure, puts down coverslip.Control centre controls the mobile movement with air pump of work head, and the pickup for being automatically performed coverslip is covered and picked up and abandons, and movement accurately and reliably, improves immunohistochemical staining treatment effeciency.

Description

A kind of the coverslip pick device and its application method of immunohistochemical staining machine
Technical field
The present invention relates to Medical Instruments technical field, the coverslip pick device of especially a kind of immunohistochemical staining machine and Its application method.
Background technique
The alternative Pathologis of immunohistochemical staining machine, the histotomy being attached on glass slide is carried out automatically it is many and diverse Manual staining process is the instrument for being automatically performed immunohistochemical staining operation.
The primary antibody reagent that immunohistochemical staining uses is very expensive.The immunohistochemical staining machine of state-of-the-art technology is using mostly When primary antibody reagent is incubated for, a lesser seal cavity is constituted, beside glass slide histotomy to reduce the use of primary antibody reagent Amount.
In addition, the immunohistochemical staining machine of state-of-the-art technology, all induces dewaxing with in-situ heat substantially and in-situ heat induction is anti- Former repair function.In order to be maintained at the equilibrium temperature of reagent and tissue during the two incubations, to reach later period best dye Color effect, designer are intended to that a lesser seal cavity can be constituted beside glass slide histotomy, so also simultaneously With the advantage for reducing reagent dosage.
It must be first attached on glass slide before tissue section strain, it is one smaller in order to be formed beside histotomy Seal cavity, immunohistochemical stainer device design application method be on tissue sections face be added dropwise a stratification cover Object；Design be using the glass slide of flanged pin frame, face covers plane coverslip method on tissue sections, or uses Plate glass slide, in the coverslip for covering flanged pin frame above, to form seal cavity between glass slide and coverslip.
Obviously the mechanical settling mode for covering slide is that most suppliers or laboratory are ready to use.This mode Increase two movements during processing: a movement is instrument after adding reagent, Yao Tianjia coverslip；Another movement is After incubation, to remove and abandon coverslip.Current slide stainer, the two movements are all using complete by hand At, or complicated clamping plate mode is used to solve.
Therefore, it is necessary to design a kind of tissue section strain instrument can be automatically performed dress that is coverslip plus setting and take abandoning It sets, to cooperate the treatment process of tissue section strain, further increases the automation and degree of intelligence of tissue section strain instrument, Tissue section strain efficiency is improved, the clients such as each laboratory, hospital use of being more convenient for.
Summary of the invention
It is an object of the present invention to provide a kind of coverslip pick devices of immunohistochemical staining machine, in immunohistochemical staining machine Coupling arrangement is equipped in work head, main body is the component for having hollow cavity, and upper end is gas-tpe fitting, transtracheal connection injection gas Pump, lower end are the coupling head of taper.Coverslip pick tool main body is connection seat, and connection seat upper section lumen and coupling head cooperate, Bottom end is silicon rubber suction cup.
Another object of the present invention is to provide a kind of application method of the coverslip pick device of immunohistochemical staining machine, work Head is moved to above coverslip, so that silicon rubber suction cup is generated negative pressure by air pump, adsorbs coverslip, and silicon rubber suction cup generates positive pressure, puts Lower coverslip.Control centre controls the mobile movement with air pump of work head, and the pickup capping and pickup for being automatically performed coverslip are lost It abandons, movement is accurate and reliable, improves immunohistochemical staining treatment effeciency.
A kind of coverslip pick device of immunohistochemical staining machine provided by the invention, the immunohistochemical staining machine are furnished with Control centre, and the work head with control centre's control X, Y, Z three-dimensional motion, horizontal work top are equipped with coverslip position Position is abandoned with waste, this coverslip pick device includes the coupling arrangement installed in the work head and is connected with coupling arrangement The coverslip pick tool connect.
The coupling arrangement main body is the component for having hollow cavity, is vertically arranged in work head, hollow cavity internal diameter For 1~5mm, main body upper end is gas-tpe fitting, and transtracheal connection injection air pump, lower body is the connection of up big and down small cone Head, height are 10~25mm.The electromagnetic switch of control centre's connection control injection air pump.
Motor, the output axis connection sliding tooth wheel of motor are installed in work head.It is equipped in the coupling arrangement main body Vertical rack gear, is engaged with gear, and control centre connects motor switch.When control centre connects motor switch, motor band movable tooth Wheel rotation, the rack gear and coupling arrangement being engaged with move up and down together.When coupling arrangement is in extreme lower position, coupling head Bottom end is in the operating position below work head, and coupling arrangement moves up when not in use, and coupling head bottom end is higher than work head bottom end.
Coverslip pick tool main body is connection seat, and connection seat is the different hollow tube of two sections of calibers, lower section lumen diameter Subtract (0~3mm) equal to coupling arrangement hollow cavity internal diameter, upper section lumen diameter is 2~5 times of lower section lumen diameter, upper section pipe It is cooperation between chamber and coupling head；It is connected between two sections of lumens through back taper chamber, the cone of back taper chamber and conical coupling head Spend identical, back taper chamber the lowermost internal diameter is less than taper coupling head bottom end minimum outer diameter, and the height of back taper chamber is equal to circular cone Shape connection grease head highness adds (1~10mm).Connection seat bottom end connects coaxial silicon rubber suction cup.The diameter of silicon rubber suction cup be coverslip most The 1/2 to 4/5 of big dimension.
When coupling head is inserted into connection seat, the back taper inner cavity surface of coupling head conical surface and connection seat is mutually pasted Interference fit is merged into, and the lumen of coupling arrangement coupling head and connection seat lower section lumen are seamlessly connected.Coupling head and connection seat Between stationary fit generate frictional force overcome coverslip pick tool gravity, in the process of running maintain coupling head and connection seat company It connects.
In the fixed cross bar with connection seat central axis in the lower end of connection seat, distal end is fixedly connected with spring pressing chamber, pressure spring Room center line and connection seat centerline parallel, have on spring pressing chamber apical pore, under have bottom outlet, there is diameter to be greater than the convex of bottom outlet in the middle part of mandril Platform, for compression spring sleeve above the boss of mandril, the following partial vertical of mandril boss stretches out spring pressing chamber bottom outlet, is butted on pressure spring at the top of pressure spring Ceiling portion, the pressure of pressure spring make mandril boss be stuck in spring pressing chamber bottom, mandril and connection seat centerline parallel, and ejector rod bottom end is lower than Silicon rubber suction cup bottom surface, the distance of the two are 3~10mm.
Coverslip pick tool on work top is placed vertically, and silicon rubber suction cup is below.
For the ease of work head be automatically performed coupling arrangement insertion coverslip pick tool connection seat and make coupling head and Connection seat connection, devises the dedicated placement position of coverslip pick tool on work top, and dedicated placement position is to be fixed on work The card for making the gap edge on table top puts seat.
One annular groove is arranged at the connection seat top of coverslip pick tool, and the height of annular groove is 4~6mm, the depth of slot For 1~2mm.
The locating piece that card puts a side is fixed on work top gap edge, and it is the card for having a groove that card, which puts an other side, The horizontal cross-section of block, groove is horizontal U-shaped, and the arc and work top gap edge of U-shaped groove bottom are tangent, and fixture block is suspended from In the notch of work top, the two-arm of groove U-shaped is stretched in notch, the spacing etc. with a thickness of 1~2mm, on the inside of two-arm of two-arm In outer diameter+(0.1~0.6mm) of connection seat annular groove slot bottom.
In the U-shaped groove of fixture block, coverslip pick tool is suspended below fixture block the annular groove of connection seat vertically.
The direction of coverslip always can be consistent with glass slide when in order to guarantee that coverslip pick tool picks up coverslip, needs The orientation of the mandril of coverslip pick tool is controlled, a mandril limiter has also been devised in dedicated placement position thus.Work There is horizontal shelf below notch on table top, mandril limiter is fixed on the shelf, and height is 8~15mm, one side Having cross section is V-arrangement limiting slot, and the maximum spacing of limiting slot V-arrangement two-arm is greater than mandril diameter, and V-Bottom portion rounded corner forms circular arc The diameter at bottom, circular arc bottom is identical as mandril diameter.Limit groove center line and card put a fixture block U-shaped groove centerline parallel, limit Channel opening direction is consistent with fixture block U-shaped groove opening direction.
When coverslip pick tool is suspended on vertically below fixture block, the mandril of side is then located at mandril limit below connection seat In the limiting slot of device, ejector rod bottom end and shelf surface distance are greater than 4.5mm.
There is the non-hydrophilic salient point or burr of high 5um~300um at the edge of the one side of the coverslip, with prevent from stacking Coverslip is mutually adhered, convenient for being picked up by silicon rubber suction cup.
A kind of application method of the coverslip pick device of immunohistochemical staining machine provided by the invention mainly includes as follows Step:
I, the capping of coverslip
I -1, the coverslip that control centre's control work head of the immunohistochemical staining machine is moved to work top picks up Above tool, the coupling head of the coupling arrangement of work head bottom is inserted into coverslip pick tool connection seat, coupling head circular cone The back taper inner cavity surface of shape surface and connection seat is bonded to each other interference fit, and frictional force connect coupling head with connection seat；
I -2, control centre's control work head of the immunohistochemical staining machine is moved on the coverslip position of work top Side, work head move down, and silicon rubber suction cup face and the coverslip surface of coupling arrangement bottom end are affixed, and control centre connects injection air pump Electromagnetic switch, injection air pump pumping make silicon rubber suction cup bottom generate negative-pressure adsorption coverslip；
I -3, control centre rises the work head of bottom end silicon rubber suction cup face sorption coverslip, is moved to that control centre is specified to want Histotomy above the glass slide for covering slide, on the perspective plane covering glass slide of coverslip；Control centre reversely connects note It emanates the electromagnetic switch of pump, injection air pump is supplied gas, and so that silicon rubber suction cup bottom is generated positive pressure, silicon rubber suction cup decontrols coverslip, coverslip It falls on glass slide, completes the capping of coverslip；
When connection seat is configured with mandril, control centre's control work head is slowly moved down, and is adjusted the position of work head, is made silicon The coverslip of glue sucker suction is in the top of the slice to be processed on glass slide, and the mandril of side is then in the load glass outside slice Above piece, ejector rod bottom end is first butted on glass slide when work head moves down, and with moving down for work head, mandril upper end is ejected spring pressing chamber Apical pore, pressure spring compressed, and ejector rod bottom end pushes down glass slide, and control centre reversely connects the electromagnetic switch of injection air pump at this time, Injection air pump is supplied gas, and silicon rubber suction cup bottom is made to generate positive pressure, and silicon rubber suction cup decontrols coverslip, and coverslip is fallen on glass slide, is completed The capping of coverslip；
II, the discarding of coverslip
II -1, control centre's control work head of the immunohistochemical staining machine is moved to the glass slide for covering and having coverslip Top, control centre's control work head slowly moves down, until the bottom surface of silicon rubber suction cup is affixed with coverslip surface；
When connection seat is configured with mandril, control centre's control work head is slowly moved down, and is adjusted the position of work head, is made silicon Glue sucker is in above coverslip, and the mandril of side is then in above the glass slide outside coverslip, mandril bottom when work head moves down End is first butted on glass slide, and with moving down for work head, mandril upper end is ejected the apical pore of spring pressing chamber, and pressure spring is compressed, mandril bottom Side pressure jail glass slide；
II -2, control centre connects the electromagnetic switch of injection air pump, and injection air pump pumping generates silicon rubber suction cup bottom negative Pressure, silicon rubber suction cup adsorb coverslip；
II -3, the work head of silicon rubber suction cup bottom end sorption coverslip is risen in control centre's control, is moved to work top Waste abandons position, and control centre reversely connects the electromagnetic switch of injection air pump, and injection air pump is supplied gas, and generates silicon rubber suction cup bottom just Pressure, silicon rubber suction cup decontrol coverslip, and coverslip is dropped；
When connection seat is configured with mandril, and control centre's control work head is begun to ramp up, silicon rubber suction cup bottom end sorption lid glass Piece rises, and because pressure spring pressure makes ejector rod bottom end ballast slide vertically downward, glass slide will not be taken up, upper with work head It rises, pressure spring gradually loosens, until ejector rod bottom end is restored at a distance from silicon rubber suction cup bottom surface, mandril disappears to the pressure of glass slide.
Have on work top and is configured with the dedicated placement position that card puts flap slide pick tool, coverslip pick tool connection The annular groove location of joint chair is put in a U-shaped groove embedded in card, is suspended in the notch of work top vertically.The immunohistochemistry dye When coverslip pick tool is installed by the control centre of color machine, the coverslip that control centre's control work head is moved to work top is picked up It takes above tool, the coupling arrangement controlled in work head moves down, and makes the conical lumen in its coupling head insertion connection seat, and right Connection seat applies downward power, and fixture block resists the annular groove upper ring surface of connection seat, prevents connection seat from moving down, conical connection The conical lumen of head and connection seat fits closely interference fit.
When the control centre of the immunohistochemical staining machine dismantles coverslip pick tool, control centre controls work head and moves The dedicated placement position of coverslip pick tool of work top is moved, the coupling arrangement controlled in work head moves down, and picks up coverslip It takes tool to enter the notch of work top, and puts a side movement to card, keep the annular groove location of connection seat recessed from the U-shaped of fixture block Channel opening side enters, in U-shaped groove.Later, work head drives coupling arrangement to move up, and card puts the ring that seat pushes down connection seat The lower anchor ring of shape slot, connection seat can not be moved up with coupling arrangement, and conical coupling head is separated with the conical lumen of connection seat, The card that coverslip pick tool hangs on work top vertically is put on seat.Work head bottom is moved away from coupling arrangement in work head Operating position is held, work head carries out other operations.
When the dedicated placement position of the coverslip pick tool of work top is also equipped with mandril limiter, control centre is placed When coverslip pick tool, in connection seat while its annular groove location enters the U-shaped groove of fixture block, side below connection seat Mandril also enter in limiting slot from the limit channel opening of mandril limiter.
Compared with prior art, a kind of coverslip pick device of immunohistochemical staining machine of the present invention and its application method Advantage are as follows: 1, control centre's control work head be automatically performed and pick up coverslip, cover in glass slide or discarding, operation accurately may be used By in place, manual operation is saved, greatly improves the efficiency of immunohistochemical staining operation；2, work head configuration coverslip picks up work Tool does not influence other operations of work head, and the coverslip pick tool can control work head certainly by control centre as needed Dynamic handling；3, structure is simple, and the structure of existing immunohistochemical staining machine can be equipped with the pickup of this coverslip without excessive change Device is suitable for popularization and application.
Detailed description of the invention
Fig. 1 is the coverslip pick device embodiment of this immunohistochemical staining machine and the overall schematic of work head；
Fig. 2 is the coverslip pick device embodiment work head and the signal of coupling arrangement section of this immunohistochemical staining machine Figure；
Fig. 3 is the coverslip pick device embodiment coverslip pick tool overall schematic of this immunohistochemical staining machine；
Fig. 4 is the coverslip pick tool diagrammatic cross-section of Fig. 3；
Fig. 5 is that the mandril of the coverslip pick device embodiment coverslip pick tool of this immunohistochemical staining machine compresses load Slide, silicon rubber suction cup prepare to pick up or put down the schematic diagram of coverslip；
Fig. 6 is that the coverslip pick device embodiment coverslip pick tool of this immunohistochemical staining machine hangs on workbench The schematic diagram of the placement position of the coverslip pick tool in face.
Figure label are as follows:
1, work head, 11, gear, 2, coupling arrangement, 21, coupling head, 22, gas-tpe fitting, 23, rack gear, 3, coverslip takes Put the connection seat of specific purpose tool, 31, silicon rubber suction cup, 32, mandril, 33, spring pressing chamber, 34, connection seat annular groove, 35, pressure spring, 4, lid Slide, 5, glass slide, 6, card put seat, 7, mandril limiter, 8, work top, 81 shelves.
Specific embodiment
Below by specific embodiments and drawings, invention is further described in detail.
The coverslip pick device embodiment of immunohistochemical staining machine
The coverslip pick device embodiment of this immunohistochemical staining machine is as shown in Figure 1, 2, the immunohistochemical staining machine Equipped with control centre, and the work head 1 with control centre's control X, Y, Z three-dimensional motion, horizontal work top are equipped with lid Slide position and waste abandon position, this coverslip pick device include the coupling arrangement 2 installed in the work head 1 and with couple The coverslip pick tool that device 2 is connected.
2 main body of coupling arrangement is the component for having hollow cavity, is vertically arranged in work head 1, this example hollow cavity Internal diameter is 3mm, and main body upper end is gas-tpe fitting 22, and transtracheal connection injection air pump, lower body is up big and down small cone Coupling head 21, height 20mm.The electromagnetic switch of control centre's connection control injection air pump.
Motor, the output axis connection sliding tooth wheel 11 of motor are installed in work head 1.Pacify in 2 main body of this example coupling arrangement It equipped with vertical rack gear 23, is engaged with gear 11, control centre connects motor switch.When control centre connects motor switch, electricity Machine band moving gear 11 rotates, and the rack gear 23 and coupling arrangement 2 being engaged with move up and down together.When coupling arrangement 2 is in minimum When position, 21 bottom end of coupling head is in the operating position of 1 lower section of work head, and coupling arrangement 2 moves up when not in use, coupling head 21 bottom ends are higher than 1 bottom end of work head.
As shown in Fig. 3,4 and 5, coverslip pick tool main body is connection seat 3, and connection seat 3 is during two sections of calibers are different Blank pipe, lower section lumen diameter are equal to 2 hollow cavity internal diameter of coupling arrangement, and upper section lumen diameter is 3 times of lower section lumen diameter, on It is cooperation between section lumen and coupling head；It is connected between two sections of lumens through back taper chamber, back taper chamber and conical coupling head 21 taper is identical, and back taper chamber the lowermost internal diameter is less than 21 bottom end minimum outer diameter of taper coupling head, the height of back taper chamber For 20mm.3 bottom end of connection seat connects coaxial silicon rubber suction cup.The diameter of silicon rubber suction cup 31 is the 2/3 of the maximum dimension of coverslip 4.
When coupling head 21 is inserted into connection seat 3, the back taper inner cavity surface of 21 conical surface of coupling head and connection seat 3 It is bonded to each other and is interference fit, and the lumen of 2 coupling head 21 of coupling arrangement and 3 lower section lumen of connection seat are seamlessly connected.Connection The frictional force that stationary fit generates between first 21 and connection seat 3 overcomes coverslip pick tool gravity, maintains connection in the process of running First 21 and connection seat 3 connection.
In the fixed cross bar with 3 central axis of connection seat in the lower end of connection seat 3, distal end is fixedly connected with spring pressing chamber 33, 33 center line of spring pressing chamber and 3 centerline parallel of connection seat, have on spring pressing chamber 33 apical pore, under have bottom outlet, have diameter in the middle part of mandril 32 Greater than the boss of bottom outlet, 35 sets of pressure spring above the boss of mandril 32, the following partial vertical of 32 boss of mandril stretches out spring pressing chamber 33 Bottom outlet, 33 top of spring pressing chamber is butted at the top of pressure spring 35, and the pressure of pressure spring 33 makes 32 boss of mandril be stuck in 33 bottom of spring pressing chamber, mandril 32 are lower than 31 bottom surface of silicon rubber suction cup with 3 centerline parallel of connection seat, 32 bottom end of mandril, and the distance of the two is 6mm.
As shown in fig. 6, this example devises the dedicated placement position of coverslip pick tool, dedicated placement position on work top The card of gap edge to be fixed on work top 8 puts seat 6.
One annular groove 34 is arranged at 3 top of connection seat of coverslip pick tool, and the height of this example annular groove 34 is 5mm, slot Depth be 1mm.
The locating piece that card puts 6 side of seat is fixed on 8 gap edge of work top, and it is to have a groove that card, which puts 6 other side of seat, The horizontal cross-section of fixture block, groove is horizontal U-shaped, and the arc and 8 gap edge of work top of U-shaped groove bottom are tangent, fixture block It is suspended from the notch of work top 8, the two-arm of groove U-shaped is stretched in notch, the spacing with a thickness of 3mm, on the inside of two-arm of two-arm Equal to the outer diameter+0.2mm of 3 annular groove of connection seat, 34 slot bottom.
In the U-shaped groove of fixture block, coverslip pick tool is suspended under fixture block the annular groove 34 of connection seat 3 vertically Side.
A mandril limiter 7 has also been devised in dedicated placement position in this example.There is horizontal put below 8 notch of work top Plate 81, mandril limiter 7 are fixed on shelf 81, height 10mm, and it is the limiting slot of V-arrangement, limit that, which there is cross section in one side, The maximum spacing of slot V-arrangement two-arm is greater than 32 diameter of mandril, and V-Bottom portion rounded corner forms circular arc bottom, the diameter and mandril of circular arc bottom 32 diameters are identical.Limit groove center line and card put 6 fixture block U-shaped groove centerline parallel of seat, limiting slot opening direction and fixture block U-shaped Slot opening direction is consistent.
When coverslip pick tool is suspended on vertically below fixture block, the mandril 32 of 3 side of connection seat is then located at mandril and limits In the limiting slot of device 7,32 bottom end of mandril and 81 surface distance of shelf are greater than 4.5mm.
There is the non-hydrophilic salient point or burr of high 5um~300um at the edge of the one side of this example coverslip 4, to prevent from stacking Coverslip be mutually adhered, convenient for being picked up by silicon rubber suction cup.
A kind of application method of the coverslip pick device of immunohistochemical staining machine provided by the invention mainly includes as follows Step:
I, the capping of coverslip 4
I -1, the coverslip that control centre's control work head 1 of the immunohistochemical staining machine is moved to work top 8 picks up It takes above tool, the coupling head 21 of the coupling arrangement 2 of 1 bottom of work head is inserted into coverslip pick tool connection seat 3, connection The back taper inner cavity surface of first 21 conical surface and connection seat 3 is bonded to each other interference fit, and frictional force makes coupling head 21 and connection Joint chair 3 connects；
I -2, control centre's control work head 1 of the immunohistochemical staining machine is moved to the coverslip position of work top 8 Top, work head 1 move down, and 31 disk of silicon rubber suction cup and 4 surface of coverslip of 2 bottom end of coupling arrangement are affixed, and control centre connects The electromagnetic switch of air pump is injected, injection air pump pumping makes 31 bottom of silicon rubber suction cup generate negative-pressure adsorption coverslip 4；
I -3, control centre rises the work head 1 of 31 face sorption coverslip 4 of bottom end silicon rubber suction cup, is moved to control centre and refers to Surely 5 top of glass slide of slide 4, the histotomy on the perspective plane covering glass slide 5 of coverslip 4 to be covered；Control centre is anti- To the electromagnetic switch for connecting injection air pump, injection air pump is supplied gas, and 31 bottom of silicon rubber suction cup is made to generate positive pressure, and silicon rubber suction cup 31 decontrols lid Slide 4, coverslip 4 are fallen on glass slide 5, complete the capping of coverslip 4；
This example connection seat 3 is configured with mandril, and control centre's control work head 1 slowly moves down, and adjusts the position of work head 1, The coverslip 4 for adsorbing silicon rubber suction cup 31 is in the top of the slice to be processed on glass slide 5, and the mandril 32 of side is then in and cuts 5 top of glass slide outside piece, 32 bottom end of mandril is first butted on glass slide 5 when work head 1 moves down, with moving down for work head 1, mandril 32 upper ends are ejected the apical pore of spring pressing chamber 33, and pressure spring 35 is compressed, and glass slide 5 is pushed down in 32 bottom end of mandril, and control centre is anti-at this time To the electromagnetic switch for connecting injection air pump, injection air pump is supplied gas, and 31 bottom of silicon rubber suction cup is made to generate positive pressure, and silicon rubber suction cup 31 decontrols lid Slide 4, coverslip 4 are fallen on glass slide 5, complete the capping of coverslip 4；As shown in Figure 5；
II, the discarding of coverslip 4
II -1, control centre's control work head 1 of the immunohistochemical staining machine is moved to the load glass for covering and having coverslip 4 5 top of piece, control centre's control work head 1 slowly moves down, until the bottom surface of silicon rubber suction cup 31 is affixed with 4 surface of coverslip；
This example connection seat 3 is configured with mandril 32, and control centre's control work head 1 slowly moves down, and adjusts the position of work head 1 It sets, silicon rubber suction cup 31 is made to be in 4 top of coverslip, the mandril 32 of side then above the glass slide 5 outside coverslip 4, works First 1 32 bottom end of mandril when moving down first is butted on glass slide 5, and with moving down for work head 1,32 upper end of mandril is ejected spring pressing chamber 33 Apical pore, pressure spring 35 are compressed, and 32 bottom end of mandril fastens glass slide 5；
II -2, control centre connects the electromagnetic switch of injection air pump, and injection air pump pumping generates 31 bottom of silicon rubber suction cup negative Pressure, silicon rubber suction cup 31 adsorb coverslip 4；
II -3, the work head 1 of 31 bottom end sorption coverslip 4 of silicon rubber suction cup is risen in control centre's control, is moved to workbench The waste in face 8 abandons position, and control centre reversely connects the electromagnetic switch of injection air pump, and injection air pump is supplied gas, and makes silicon rubber suction cup 31 Bottom generates positive pressure, and silicon rubber suction cup 31 decontrols coverslip 4, and coverslip 4 is dropped；
This example connection seat 3 is configured with mandril 32, when control centre's control work head 1 is begun to ramp up, 31 bottom end of silicon rubber suction cup Sorption coverslip 4 rises, and because 35 pressure of pressure spring makes 32 bottom end of mandril ballast slide 5 vertically downward, glass slide 5 will not be taken up, With the rising of work head 1, pressure spring 35 gradually loosens, until the mandril at a distance from 31 bottom surface of silicon rubber suction cup is restored in 32 bottom end of mandril The pressure of 32 pairs of glass slides 4 disappears.
There is the dedicated placement position for being configured with the coverslip pick tool that card puts seat 6 on work top 8, coverslip picks up work 34 position of annular groove of tool connection seat 3 is put in the U-shaped groove of seat 6 embedded in card, is suspended in the notch of work top 8 vertically.Institute When stating control centre's installation coverslip pick tool of immunohistochemical staining machine, control centre's control work head 1 is moved to work Above the coverslip pick tool of table top 8, the coupling arrangement 2 controlled in work head 1 is moved down, its coupling head 21 is made to be inserted into connection seat Conical lumen in 3, and apply downward power to connection seat 3, fixture block resists 34 upper ring surface of annular groove of connection seat 3, makes to join Joint chair 3 cannot move down, and the conical lumen of conical coupling head 21 and connection seat 3 fits closely interference fit.
When the control centre of the immunohistochemical staining machine dismantles coverslip pick tool, control centre controls work head 1 It is moved to the dedicated placement position of coverslip pick tool of work top 8, the coupling arrangement 2 controlled in work head 1 moves down, and makes lid glass Piece pick tool enters the notch of work top 8, and puts the movement of 6 side of seat to card, makes 34 position of annular groove of connection seat 3 from card The U-shaped groove opening side of block enters, in U-shaped groove.Later, work head 1 drives coupling arrangement 2 to move up, and card puts seat 6 Fixture block pushes down the lower anchor ring of the annular groove 34 of connection seat 3, and connection seat 3 can not be moved up with coupling arrangement 2, conical coupling head 21 It is separated with the conical lumen of connection seat 3, the card that coverslip pick tool hangs on work top 8 vertically is put on seat 6.Work head 1 bottom end operating position of work head is moved away from coupling arrangement 2 on 1, work head 1 carries out other operations.It is inhaled as work head 1 couples Tube head executes instrument and draws reagent solution from the kit on work top 8, moves to above the glass slide of incubation slot, carrying Reagent is added dropwise on the histotomy of slide.
This example is also equipped with mandril limiter, control centre in the dedicated placement position of the coverslip pick tool of work top 8 When placing coverslip pick tool, while the annular groove of connection seat 3 enters the U-shaped groove of fixture block, 3 lower section side of connection seat Mandril 32 also enter in limiting slot from the limit channel opening of mandril limiter.
Above-described embodiment is only further described the purpose of the present invention, technical scheme and beneficial effects specific A example, present invention is not limited to this.All any modifications made within the scope of disclosure of the invention, change equivalent replacement Into etc., it is all included in the scope of protection of the present invention.

Claims (10)

1.一种免疫组化染色机的盖玻片拾取装置，所述免疫组化染色机配有控制中心，且具有控制中心控制X、Y、Z三维运动的工作头(1)，水平的工作台面(8)上设有盖玻片位和废物丢弃位，其特征在于：1. A cover glass pick-up device of an immunohistochemical staining machine, the immunohistochemical staining machine is equipped with a control center, and has a work head (1) that the control center controls the three-dimensional movement of X, Y, Z, and the horizontal work The table top (8) is provided with a cover glass position and a waste disposal position, characterized in that: 所述盖玻片拾取装置包括在所述工作头(1)上安装的联接装置(2)和可与联接装置(2)相连接的盖玻片拾取工具；The cover glass pickup device comprises a coupling device (2) mounted on the working head (1) and a cover glass pickup tool that can be connected with the coupling device (2); 所述联接装置(2)主体为有中空腔体的部件，其竖直安装于工作头(1)上，中空腔体内径为1～5mm，其上端为气管接头(22)，经气管连接注射气泵，主体下端为上大下小圆锥形的联接头(21)，其高度为10～25mm；控制中心连接控制注射气泵的电磁开关；The main body of the coupling device (2) is a component with a hollow cavity, which is vertically installed on the working head (1), the inner diameter of the hollow cavity is 1 to 5 mm, and the upper end of the coupling device is a tracheal joint (22), which is connected to the trachea for injection The air pump, the lower end of the main body is a connecting head (21) with a large upper and a lower small conical, and its height is 10-25 mm; the control center is connected to the electromagnetic switch for controlling the injection air pump; 盖玻片拾取工具主体为联接座(3)，联接座(3)为两段管径不同的中空管，下段管腔内径等于联接装置(2)中空腔体内径减(0～3mm)，上段管腔内径为下段管腔内径的2～5倍，上段管腔与联接头(21)之间为动配合；二段管腔之间经倒锥形腔连接，倒锥形腔与圆锥形联接头(21)的锥度相同，倒锥形腔最下端的内径小于锥形联接头(21)底端最小外径，倒锥形腔的高度等于圆锥形联接头(21)高度加(1～10mm)；联接座(3)底端连接同轴的硅胶吸盘(31)，硅胶吸盘(31)的直径为盖玻片(4)最大线度的1/2至4/5；The main body of the cover glass pick-up tool is a coupling seat (3), the coupling seat (3) is two sections of hollow tubes with different pipe diameters, and the inner diameter of the lower section is equal to the inner diameter of the hollow cavity of the coupling device (2) minus (0-3 mm), The inner diameter of the upper lumen is 2 to 5 times the inner diameter of the lower lumen, and the upper lumen and the coupling head (21) are in dynamic fit; The taper of the coupling head (21) is the same, the inner diameter of the lowermost end of the inverted conical cavity is smaller than the minimum outer diameter of the bottom end of the conical coupling head (21), and the height of the inverted conical cavity is equal to the height of the conical coupling head (21) plus (1～ 10mm); the bottom end of the connecting seat (3) is connected to a coaxial silicone suction cup (31), and the diameter of the silicone suction cup (31) is 1/2 to 4/5 of the maximum linearity of the cover glass (4); 当联接头(21)插入到联接座(3)内，联接头(21)圆锥形表面与联接座(3)的倒锥形腔内表面相互贴合并为过盈配合，且联接装置(2)的管腔与联接座(3)下段管腔无缝连接。When the coupling head (21) is inserted into the coupling seat (3), the conical surface of the coupling head (21) and the inner surface of the inverted conical cavity of the coupling seat (3) abut each other and form an interference fit, and the coupling device (2) The lumen of the connecting seat (3) is seamlessly connected with the lumen of the lower section. 2.根据权利要求1所述的免疫组化染色机的盖玻片拾取装置，其特征在于：2. the cover glass pickup device of immunohistochemical staining machine according to claim 1, is characterized in that: 所述工作头(1)上安装有电机，电机的输出轴连接驱动齿轮(11)；所述联接装置(2)主体上安装有竖直的齿条(23)，与电机的齿轮(11)啮合，控制中心连接电机开关；当控制中心接通电机开关，电机带动齿轮(11)转动，与之啮合的齿条(23)和联接装置(2)一起上下移动，当联接装置(2)处于最低位置时，其联接头(21)底端处于工作头(1)下方的工作位置，联接装置(2)不使用时上移，其联接头(21)底端高于工作头(1)底端。A motor is installed on the working head (1), and the output shaft of the motor is connected with a driving gear (11); a vertical rack (23) is installed on the main body of the coupling device (2), which is connected with the gear (11) of the motor. Meshing, the control center is connected to the motor switch; when the control center turns on the motor switch, the motor drives the gear (11) to rotate, and the meshed rack (23) and the coupling device (2) move up and down together. When the coupling device (2) is in In the lowest position, the bottom end of the coupling head (21) is at the working position below the working head (1), and the coupling device (2) moves up when not in use, and the bottom end of the coupling head (21) is higher than the bottom of the working head (1). end. 3.根据权利要求1所述的免疫组化染色机的盖玻片拾取装置，其特征在于：3. the cover glass pickup device of immunohistochemical staining machine according to claim 1, is characterized in that: 所述联接座(3)的下端固定与联接座(3)中心线垂直的横杆，其远端固定连接压簧室(33)，压簧室(33)中心线与联接座(3)中心线平行，压簧室(33)上有顶孔、下有底孔，顶杆(32)中部有直径大于底孔的凸台，压簧(35)套在顶杆(32)的凸台上方，顶杆(32)凸台以下部份垂直伸出压簧室(33)底孔，压簧(35)顶部抵于压簧室(33)顶部，压簧(35)的压力使顶杆(32)凸台卡于压簧室(33)底部，顶杆(32)与联接座(3)中心线平行，顶杆(32)底端低于硅胶吸盘(31)底面，二者的距离为3～10mm。The lower end of the coupling seat (3) is fixed to a cross bar perpendicular to the center line of the coupling seat (3), and the distal end thereof is fixedly connected to the compression spring chamber (33), the center line of the compression spring chamber (33) and the center of the coupling seat (3) The lines are parallel, the compression spring chamber (33) has a top hole and a bottom hole, the middle of the ejector rod (32) has a boss with a diameter larger than the bottom hole, and the compression spring (35) is sleeved above the boss of the ejector rod (32). , the lower part of the boss of the ejector rod (32) protrudes from the bottom hole of the compression spring chamber (33) vertically, the top of the compression spring (35) touches the top of the compression spring chamber (33), and the pressure of the compression spring (35) makes the ejector rod (35). 32) The boss is clamped at the bottom of the compression spring chamber (33), the top rod (32) is parallel to the center line of the connecting seat (3), the bottom end of the top rod (32) is lower than the bottom surface of the silicone suction cup (31), and the distance between the two is 3～10mm. 4.根据权利要求1所述的免疫组化染色机的盖玻片拾取装置，其特征在于：4. the cover glass pickup device of immunohistochemical staining machine according to claim 1, is characterized in that: 在工作台面(8)上的盖玻片拾取工具竖直放置，硅胶吸盘(31)处于下方。The cover glass pick-up tool on the work surface (8) is placed vertically with the silicone suction cup (31) below. 5.根据权利要求4所述的免疫组化染色机的盖玻片拾取装置，其特征在于：5. The cover glass pickup device of the immunohistochemical staining machine according to claim 4, is characterized in that: 在工作台面(8)上设计了盖玻片拾取工具的专用放置位，专用放置位为固定于工作台面(8)上的缺口边缘的卡放座(6)；A special placement position for the cover glass pick-up tool is designed on the work table (8), and the special placement position is a clip seat (6) fixed on the edge of the notch on the work table (8); 盖玻片拾取工具的联接座(3)上部有一环形槽(34)，环形槽(34)的高度为4～6mm，其槽的深度为1～2mm；There is an annular groove (34) on the upper part of the coupling seat (3) of the cover glass pick-up tool, the height of the annular groove (34) is 4-6 mm, and the depth of the groove is 1-2 mm; 卡放座(6)一侧的定位块固定于工作台面(8)缺口边缘，卡放座(6)另一侧为有一凹槽的卡块，凹槽的水平截面为横置的U形，U形凹槽底部的弧形与工作台面(8)缺口边缘相切，卡块悬于工作台面(8)的缺口内，凹槽U形的两臂伸向缺口内，两臂的厚度为1～2mm，两臂内侧的间距等于联接座(3)环形槽(34)槽底的外径加(0.1～0.6mm)；The positioning block on one side of the clamping seat (6) is fixed on the edge of the notch of the work table (8), and the other side of the clamping seat (6) is a clamping block with a groove, and the horizontal section of the groove is a horizontal U-shape, The arc at the bottom of the U-shaped groove is tangent to the notch edge of the working table (8), the clamping block is suspended in the notch of the working table (8), the two arms of the U-shaped groove extend into the notch, and the thickness of the two arms is 1 ～2mm, the distance between the inner sides of the two arms is equal to the outer diameter of the bottom of the annular groove (34) of the connecting seat (3) plus (0.1～0.6mm); 联接座(3)的环形槽(34)嵌于卡块的U形凹槽内，盖玻片拾取工具竖直悬挂在卡块下方。The annular groove (34) of the coupling seat (3) is embedded in the U-shaped groove of the clamping block, and the cover glass picking tool is vertically suspended under the clamping block. 6.根据权利要求5所述的免疫组化染色机的盖玻片拾取装置，其特征在于：6. The coverslip pick-up device of an immunohistochemical staining machine according to claim 5, characterized in that: 在专用放置位还设计了一个顶杆限位器(7)；工作台面(8)上的缺口的下方有水平的搁板(81)，顶杆限位器(7)固定在搁板(81)上，其高度为8～15mm，其一侧有横截面为V形的限位槽，限位槽V形两臂的最大间距大于顶杆(32)直径，V形底部倒圆角形成圆弧底，圆弧底直径与顶杆(32)直径相同；限位槽中心线与卡放座(6)卡块U形凹槽中心线平行，限位槽开口方向与卡块U形凹槽开口方向一致；A top rod stopper (7) is also designed in the special placement position; there is a horizontal shelf (81) below the notch on the work surface (8), and the top rod stopper (7) is fixed on the shelf (81). ), its height is 8-15mm, and its side has a V-shaped limit groove in cross-section, the maximum distance between the two arms of the V-shaped limit groove is greater than the diameter of the top rod (32), and the rounded corners of the V-shaped bottom form a circle Arc bottom, the diameter of the arc bottom is the same as the diameter of the ejector rod (32); the center line of the limit groove is parallel to the center line of the U-shaped groove of the clamping block of the clamping seat (6), and the opening direction of the limit groove is the same as that of the U-shaped groove of the clamping block. The opening direction is the same; 当盖玻片拾取工具竖直悬挂在卡块下方，联接座(3)下方一侧的顶杆(32)则位于顶杆限位器(7)的限位槽内，顶杆(32)底端与搁板(81)表面距离大于4.5mm。When the cover glass pick-up tool is vertically suspended below the clamping block, the ejector rod (32) on the lower side of the coupling seat (3) is located in the limit groove of the ejector rod limiter (7). The distance between the end and the surface of the shelf (81) is greater than 4.5mm. 7.根据权利要求1至6中任一项所述的免疫组化染色机的盖玻片拾取装置，其特征在于：7. The cover glass pickup device of the immunohistochemical staining machine according to any one of claims 1 to 6, characterized in that: 所述盖玻片(4)的一面的边缘有高5um～300um的非亲水的凸点或凸纹。The edge of one side of the cover glass (4) has non-hydrophilic bumps or ridges with a height of 5um-300um. 8.根据权利要求1或2所述的免疫组化染色机的盖玻片拾取装置的使用方法，其特征在于包括如下步骤：8. the using method of the cover glass pick-up device of immunohistochemical staining machine according to claim 1 and 2 is characterized in that comprising the steps: Ⅰ、盖玻片(4)的加盖Ⅰ. Cover glass (4) Ⅰ-1、所述免疫组化染色机的控制中心控制工作头(1)移动到工作台面(8)的盖玻片拾取工具上方，将工作头(1)底部的联接装置(2)的联接头(21)插入盖玻片拾取工具联接座(3)内，联接头(21)圆锥形表面与联接座(3)的倒锥形腔内表面相互贴合过盈配合，摩擦力使联接头(21)与联接座(3)连接；Ⅰ-1. The control center of the immunohistochemical staining machine controls the working head (1) to move above the cover glass pick-up tool on the work surface (8), and connect the coupling device (2) at the bottom of the working head (1) to the top of the cover glass pick-up tool. The joint (21) is inserted into the cover glass pick-up tool coupling seat (3), the conical surface of the coupling head (21) and the inner surface of the inverted conical cavity of the coupling seat (3) are in interference fit with each other, and the friction force makes the coupling head (21) is connected with the connecting seat (3); Ⅰ-2、所述免疫组化染色机的控制中心控制工作头(1)移动到工作台面(8)的盖玻片位上方，工作头(1)下移，联接装置(2)底端的硅胶吸盘(31)面与盖玻片(4)表面相贴，控制中心接通注射气泵的电磁开关，注射气泵抽气使硅胶吸盘(31)底产生负压吸附盖玻片(4)；Ⅰ-2. The control center of the immunohistochemical staining machine controls the working head (1) to move above the cover glass position on the work surface (8), the working head (1) moves down, and the silica gel at the bottom of the connecting device (2) is connected The surface of the suction cup (31) is in contact with the surface of the cover glass (4), the control center turns on the electromagnetic switch of the injection air pump, and the injection air pump draws air to generate a negative pressure at the bottom of the silicone suction cup (31) to adsorb the cover glass (4); Ⅰ-3、控制中心升起底端的硅胶吸盘(31)吸着盖玻片(4)的工作头(1)、移动到控制中心指定要加盖玻片(4)的载玻片(5)上方，盖玻片(4)的投影面覆盖载玻片(5)上的组织切片；控制中心反向接通注射气泵的电磁开关，注射气泵送气，使硅胶吸盘(31)底产生正压，硅胶吸盘(31)放开盖玻片(4)，盖玻片(4)落在载玻片(5)上，完成盖玻片(4)的加盖；Ⅰ-3. The silicone suction cup (31) at the bottom of the control center lifts up to suck the working head (1) of the cover glass (4), and moves it to the top of the slide glass (5) designated by the control center to add the cover glass (4). , the projection surface of the cover glass (4) covers the tissue section on the slide glass (5); the control center reversely turns on the electromagnetic switch of the injection air pump, and the injection air pump delivers air to generate positive pressure at the bottom of the silicone suction cup (31), and the silicone The suction cup (31) releases the cover glass (4), the cover glass (4) falls on the slide glass (5), and the covering of the cover glass (4) is completed; Ⅱ、盖玻片的丢弃Ⅱ. Disposal of coverslips Ⅱ-1、所述免疫组化染色机的控制中心控制工作头(1)移动到加盖有盖玻片(4)的载玻片(5)上方，控制中心控制工作头(1)缓慢下移，直至硅胶吸盘(31)的底面与盖玻片(4)表面相贴；II-1. The control center of the immunohistochemical staining machine controls the working head (1) to move above the glass slide (5) covered with the cover glass (4), and the control center controls the working head (1) to move down slowly. move until the bottom surface of the silicone suction cup (31) is in contact with the surface of the cover glass (4); Ⅱ-2、控制中心接通注射气泵的电磁开关，注射气泵抽气，使硅胶吸盘(31)底产生负压，硅胶吸盘(31)吸附盖玻片(4)；Ⅱ-2. The control center turns on the electromagnetic switch of the injection air pump, and the injection air pump draws air, so that a negative pressure is generated at the bottom of the silica gel suction cup (31), and the silica gel suction cup (31) adsorbs the cover glass (4); Ⅱ-3、控制中心控制升起硅胶吸盘(31)底端吸着盖玻片(4)的工作头(1)，移动到工作台面(8)的废物丢弃位，控制中心反向接通注射气泵的电磁开关，注射气泵送气，使硅胶吸盘(31)底产生正压，硅胶吸盘(31)放开盖玻片(4)，盖玻片(4)被丢弃。Ⅱ-3. The control center controls to raise the working head (1) of the silicone suction cup (31), which sucks the cover glass (4) at the bottom, and moves it to the waste disposal position on the work surface (8). The control center turns on the injection pump in reverse. The electromagnetic switch is activated, and the injection air pump supplies air to generate positive pressure at the bottom of the silicone suction cup (31), the silicone suction cup (31) releases the cover glass (4), and the cover glass (4) is discarded. 9.根据权利要求8所述的免疫组化染色机的盖玻片拾取装置的使用方法，其特征在于：9. the using method of the cover glass pickup device of immunohistochemical staining machine according to claim 8, is characterized in that: 当所述联接座(3)的下端固定与联接座(3)中心线垂直的横杆，其远端固定连接压簧室(33)，压簧室(33)中心线与联接座(3)中心线平行，压簧室(33)上有顶孔、下有底孔，顶杆(32)中部有直径大于底孔的凸台，压簧(35)套在顶杆(32)的凸台上方，顶杆(32)凸台以下部份垂直伸出压簧室(33)底孔，压簧(35)顶部抵于压簧室(33)顶部，压簧(35)的压力使顶杆(32)凸台卡于压簧室(33)底部，顶杆(32)与联接(3)中心线平行，顶杆(32)底端低于硅胶吸盘(31)底面，二者的距离为3～10mm；When the lower end of the coupling seat (3) is fixed to a cross bar perpendicular to the center line of the coupling seat (3), the distal end of the connecting seat (3) is fixedly connected to the compression spring chamber (33), and the center line of the compression spring chamber (33) is connected to the coupling seat (3) The center lines are parallel, the compression spring chamber (33) has a top hole and a bottom hole, the middle of the ejector rod (32) has a boss with a diameter larger than the bottom hole, and the compression spring (35) is sleeved on the boss of the ejector rod (32). Above, the part below the boss of the ejector rod (32) protrudes from the bottom hole of the compression spring chamber (33) vertically, the top of the compression spring (35) touches the top of the compression spring chamber (33), and the pressure of the compression spring (35) makes the ejector rod (32) The boss is clamped at the bottom of the compression spring chamber (33), the top rod (32) is parallel to the center line of the connection (3), the bottom end of the top rod (32) is lower than the bottom surface of the silicone suction cup (31), and the distance between the two is 3～10mm; 所述步骤Ⅰ-3控制中心控制工作头(1)缓慢下移，调节工作头(1)的位置，使硅胶吸盘(31)吸附的盖玻片(4)处于载玻片(5)上的待处理切片的上方，一侧的顶杆(32)则处于切片外的载玻片(5)上方，工作头(1)下移时顶杆(32)底端先抵于载玻片(5)，随着工作头(1)的下移，顶杆(32)上端被顶出压簧室(33)的顶孔，压簧(35)被压缩，顶杆(32)底端压住载玻片(5)，此时控制中心反向接通注射气泵的电磁开关，注射气泵送气，使硅胶吸盘(31)底产生正压，硅胶吸盘(31)放开盖玻片(4)，盖玻片(4)落在载玻片(5)上，完成盖玻片(4)的加盖；The step I-3 control center controls the working head (1) to move down slowly, adjusts the position of the working head (1), so that the cover glass (4) adsorbed by the silica gel suction cup (31) is placed on the slide glass (5). Above the slice to be processed, the ejector rod (32) on one side is above the slide glass (5) outside the slice, and the bottom end of the ejector rod (32) first touches the slide glass (5) when the working head (1) moves down. ), with the downward movement of the working head (1), the upper end of the ejector rod (32) is pushed out of the top hole of the compression spring chamber (33), the compression spring (35) is compressed, and the bottom end of the ejector rod (32) presses the load Glass slide (5), at this time, the control center turns on the electromagnetic switch of the injection air pump in reverse, and the injection air pump supplies air to generate positive pressure at the bottom of the silicone suction cup (31), the silicone suction cup (31) releases the cover glass (4), and the The glass slide (4) falls on the slide glass (5), and the covering of the cover glass (4) is completed; 所述步骤Ⅱ-1，控制中心控制工作头(1)缓慢下移，调节工作头(1)的位置，使硅胶吸盘(31)处于盖玻片(4)上方，一侧的顶杆(32)则处于盖玻片(4)外的载玻片(5)上方，工作头(1)下移时顶杆(32)底端先抵于载玻片(5)，随着工作头(1)的下移，顶杆(32)上端被顶出压簧室(33)的顶孔，压簧(35)被压缩，顶杆(32)底端压牢载玻片(5)；In the step II-1, the control center controls the working head (1) to move down slowly, and adjusts the position of the working head (1) so that the silicone suction cup (31) is above the cover glass (4), and the ejector rod (32) on one side is positioned above the cover glass (4). ) is placed above the glass slide (5) outside the cover glass (4). ) moves down, the upper end of the ejector rod (32) is pushed out of the top hole of the compression spring chamber (33), the compression spring (35) is compressed, and the bottom end of the ejector rod (32) presses the glass slide (5) firmly; 所述步骤Ⅱ-3，控制中心控制工作头(1)开始上升时，硅胶吸盘(31)底端吸着盖玻片(4)升起，因压簧(35)压力使顶杆(32)底端垂直向下压载玻片(5)，载玻片(5)不会被带起，随着工作头(1)的上升，压簧(35)逐渐放松，至顶杆(32)底端恢复与硅胶吸盘(31)底面的距离，顶杆(32)对载玻片(5)的压力消失。In the step II-3, when the control center controls the working head (1) to start to rise, the bottom end of the silicone suction cup (31) sucks the cover glass (4) to lift up, and the top rod (32) bottoms due to the pressure of the compression spring (35). Press the glass slide (5) vertically downward, the glass slide (5) will not be lifted up, and as the working head (1) rises, the compression spring (35) is gradually loosened, and reaches the bottom end of the ejector rod (32). The distance from the bottom surface of the silicone suction cup (31) is restored, and the pressure of the ejector rod (32) on the slide glass (5) disappears. 10.根据权利要求8所述的免疫组化染色机的盖玻片拾取装置的使用方法，其特征在于：10. The using method of the cover glass pickup device of the immunohistochemical staining machine according to claim 8, characterized in that: 在工作台面(8)上有盖玻片拾取工具专用放置位，专用放置位有固定于工作台面(8)上的缺口边缘的卡放座(6)；On the worktable (8), there is a special placement position for a cover glass pick-up tool, and the special placement position is provided with a clamping seat (6) fixed on the edge of the notch on the worktable (8); 盖玻片拾取工具的联接座(3)上部有一环形槽(34)，环形槽(34)的高度为4～6mm，其槽的深度为1～2mm；There is an annular groove (34) on the upper part of the coupling seat (3) of the cover glass pick-up tool, the height of the annular groove (34) is 4-6 mm, and the depth of the groove is 1-2 mm; 卡放座(6)一侧的定位块固定于工作台面(8)缺口边缘，卡放座(6)另一侧为有一凹槽的卡块，凹槽的水平截面为横置的U形，U形凹槽底部的弧形与工作台面(8)缺口边缘相切，卡块悬于工作台面(8)的缺口内，凹槽U形的两臂伸向缺口内，两臂的厚度为2～4mm，两臂内侧的间距等于联接座(3)环形槽(34)槽底的外径+(0.1～0.6mm)；The positioning block on one side of the clamping seat (6) is fixed on the edge of the notch of the work table (8), and the other side of the clamping seat (6) is a clamping block with a groove, and the horizontal section of the groove is a horizontal U-shape, The arc at the bottom of the U-shaped groove is tangent to the edge of the notch of the working table (8), the clamping block is suspended in the notch of the working table (8), the two arms of the U-shaped groove extend into the notch, and the thickness of the two arms is 2 ~4mm, the distance between the inner sides of the two arms is equal to the outer diameter of the bottom of the ring groove (34) of the coupling seat (3) + (0.1 ~ 0.6mm); 盖玻片拾取工具联接座的环形槽(34)部位嵌于卡放座(6)的U形凹槽内，竖直悬挂在工作台面(8)的缺口内；所述免疫组化染色机的控制中心安装盖玻片拾取工具时，控制中心控制工作头(1)移动到工作台面(8)的盖玻片拾取工具上方，控制工作头(1)上的联接装置(2)下移，使其联接头(3)插入联接座(3)内的圆锥形管腔，并对联接座(3)施加向下的力，卡块抵住联接座(3)的环形槽(34)上环面，使联接座(3)不能下移，圆锥形的联接头(21)与联接座(3)的圆锥形管腔紧密贴合并过盈配合；The part of the annular groove (34) of the coupling seat of the cover glass pick-up tool is embedded in the U-shaped groove of the clamping seat (6), and is vertically suspended in the notch of the working table (8); When the control center installs the cover glass pick-up tool, the control center controls the work head (1) to move above the cover glass pick-up tool on the work surface (8), and controls the coupling device (2) on the work head (1) to move down, so that the The coupling head (3) is inserted into the conical lumen in the coupling seat (3), and a downward force is applied to the coupling seat (3), and the clamping block is pressed against the upper annular surface of the annular groove (34) of the coupling seat (3). , so that the coupling seat (3) cannot be moved down, and the conical coupling head (21) is closely fitted and interfered with the conical lumen of the coupling seat (3); 所述免疫组化染色机的控制中心拆卸盖玻片拾取工具时，控制中心控制工作头(1)移动到工作台面(8)的盖玻片拾取工具专用放置位，控制工作头(1)上的联接装置(2)下移，使盖玻片拾取工具进入工作台面(8)的缺口，并向卡放座(6)一侧移动，使联接座(3)的环形槽(34)部位从卡块的U形凹槽开口一侧进入、嵌于U形凹槽内；之后，工作头(1)带动联接装置(2)上移，卡放座(6)的卡块压住联接座(3)的环形槽(34)的下环面，联接座(3)无法随联接装置(2)上移，圆锥形的联接头(21)与联接座(3)的圆锥形管腔分离，盖玻片拾取工具竖直悬挂于工作台面(8)的卡放座(6)上；工作头(1)上的联接装置(2)上移，离开工作头(1)底端工作位置，工作头(1)进行其它操作。When the control center of the immunohistochemical staining machine disassembles the cover glass pick-up tool, the control center controls the work head (1) to move to the special placement position of the cover glass pick-up tool on the work surface (8), and controls the work head (1) The coupling device (2) moves down, so that the cover glass pick-up tool enters the notch of the work surface (8), and moves to the side of the clamping seat (6), so that the annular groove (34) of the coupling seat (3) is removed from the The opening side of the U-shaped groove of the clamping block enters and is embedded in the U-shaped groove; after that, the working head (1) drives the coupling device (2) to move up, and the clamping block of the clamping seat (6) presses the coupling seat ( 3) On the lower annular surface of the annular groove (34), the coupling seat (3) cannot move up with the coupling device (2), and the conical coupling head (21) is separated from the conical lumen of the coupling seat (3). The slide pick-up tool is vertically suspended on the clamping seat (6) of the working table (8); the coupling device (2) on the working head (1) moves upwards and leaves the working position at the bottom end of the working head (1). (1) Perform other operations.
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