CN109575101A - A kind of highly effective extraction method of teasel root saponin(e VI - Google Patents
A kind of highly effective extraction method of teasel root saponin(e VI Download PDFInfo
- Publication number
- CN109575101A CN109575101A CN201811627297.9A CN201811627297A CN109575101A CN 109575101 A CN109575101 A CN 109575101A CN 201811627297 A CN201811627297 A CN 201811627297A CN 109575101 A CN109575101 A CN 109575101A
- Authority
- CN
- China
- Prior art keywords
- teasel root
- saponin
- extraction
- root saponin
- highly effective
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses the highly effective extraction methods of teasel root saponin(e VI a kind of, belong to pharmaceutical technology field.The extracting method of teasel root saponin(e VI is using teasel root medicinal material as raw material, specifically includes the following steps: firstly, after taking teasel root medicinal material to be crushed, sieving obtains Himalayan Teasel Root；Then, the Extraction solvent and the examination of soak extraction time, acquisition for taking above-mentioned Himalayan Teasel Root to mix by the second alcohol and water to various concentration extract optimum extraction solvent and the extraction time of teasel root saponin(e VI from teasel root；Then, teasel root saponin(e VI in teasel root is then extracted using column extraction method, the extracting solution containing teasel root saponin(e VI can be obtained.It is carried out under extracting method room temperature of the invention, easy to operate, required solvent volume is small, and extraction cost is low, and extraction efficiency is high.Teasel root saponin(e VI is extracted so as to solving the problems, such as tradition from teasel root, and that there are extraction efficiencies is lower.
Description
Technical field
The present invention relates to pharmaceutical technology field, the highly effective extraction method of specifically a kind of teasel root saponin(e VI.
Background technique
Teasel root saponin (Asperosaponin VI) also known as akebiasaponin D, or be teasel root saponin(e C, it is to be passed from China
A kind of triterpene saponin componds isolated are extracted in the root of system Chinese herbal medicine teasel root plant, are the main actives of teasel root.
Wherein, teasel root is a kind of herbaceos perennial, is mainly grown in moist mountainous region area.In Chinese tradition medication history, river
Teasel root can be used as a kind of tonic, analgesic and anti-inflammatory agent.
In addition, teasel root saponin is the representative compound in saponin component, pharmacological action is extensive, with the total soap of ginseng
Glycosides have similar pharmacological activity, neuroprotection, myocardial preservation, pre- preventing bone rarefaction, anti-apoptotic, in terms of
Pharmacological activity cause the concern of numerous scholars, research and development value with higher.
Mainly have to the extracting method of teasel root saponin(e VI at present: the water that (one) adds 15 times and 10 times amounts respectively extracts
2 times, each extraction time is about 2 hours, can extract teasel root saponin(e VI；(2) water is added to dissolve dipsacus total saponin,
It is precipitated, is repeated above-mentioned by cooling down perhaps to reduce to filter or be centrifuged after solvent polarity is sufficiently precipitated to teasel root saponin(e VI
After operation 1-3 times, teasel root saponin(e VI monomer can be obtained.But, both methods is required to consume a large amount of solvent, and time-consuming
Length, extraction efficiency are lower.Therefore the extracting method to teasel root saponin(e VI is also needed to improve, to improve its extraction efficiency.
Summary of the invention
The purpose of the present invention is to provide the highly effective extraction methods of teasel root saponin(e VI a kind of, to solve in above-mentioned background technique
The problem of proposition.
To achieve the above object, the invention provides the following technical scheme:
A kind of highly effective extraction method of teasel root saponin(e VI, using teasel root medicinal material as raw material, comprising the following steps:
(1) after taking teasel root medicinal material to be crushed, sieving obtains Himalayan Teasel Root；
(2) above-mentioned Himalayan Teasel Root is taken to be immersed in the Extraction solvent of second alcohol and water mixing；
(3) Himalayan Teasel Root of above-mentioned immersion is used into column extraction method, the extracting solution containing teasel root saponin(e VI can be obtained；
The volumetric concentration of ethyl alcohol is 40%-80% in the Extraction solvent that second alcohol and water mixes in the step (2).
A kind of preferred embodiment that the present invention uses, the middle mesh number being sieved of the step (1) is 20-60 mesh.
Another preferred embodiment that the present invention uses, the step (2) the middle time impregnated is 2-4h, and the temperature of immersion is
20-30℃。
Another preferred embodiment that the present invention uses, the step (2) the middle time impregnated is 3h.
Another preferred embodiment that the present invention uses, the volumetric concentration of ethyl alcohol in the Extraction solvent of second alcohol and water mixing
It is 60%.
Another preferred embodiment that the present invention uses, the concentration of Himalayan Teasel Root in the Extraction solvent of second alcohol and water mixing
For 0.08-0.12g/mL.
Another preferred embodiment that the present invention uses, step (3) the center pillar extraction method comprise the concrete steps that: by above-mentioned second
The Extraction solvent of alcohol and water mixing is placed in that eluent flow rate is 1-3BV/h, diameter height ratio is 1:(4-6) Himalayan Teasel Root column in continuous
Disconnected saponin(e VI is extracted, and after collecting preceding 2-4 MV extracting solution and merging, mentioning containing teasel root saponin(e VI can be obtained
Take liquid.
Compared with prior art, the beneficial effects of the present invention are:
Extracting method of the invention is simple, uses post processing extraction solvent consumption it is small it is low, extraction cost is low, and teasel root saponin(e VI
Extraction efficiency is high.Wherein, the present invention is using teasel root medicinal material as raw material, by using the Extraction solvent of second alcohol and water mixing as continuous
The Extraction solvent of disconnected saponin(e VI, and the concentration of ethyl alcohol is controlled 60% in the Extraction solvent that second alcohol and water is mixed, and will be continued
Disconnected powder is controlled in the soaking time of Extraction solvent in 3h, can greatly improve the recovery rate and extraction efficiency of teasel root saponin(e VI.
In addition, being extracted with column extraction method to teasel root saponin(e VI according to technical solution provided by the invention, as long as collecting
The first two MV can make the extracted amount of teasel root saponin(e VI reach 91.6% of teasel root saponin(e VI total amount in teasel root, so as to big
Reduce the usage amount of Extraction solvent greatly, and improves the extraction efficiency of teasel root saponin(e VI.
Detailed description of the invention
Fig. 1 is the thin-layer chromatography colour developing figure of extracting solution and standard items stock solution.
Fig. 2 is the liquid chromatogram of standard items stock solution.
Fig. 3 is the linear relationship chart of teasel root saponin(e VI concentration and chromatographic peak area.
The chromatography that Fig. 4 is teasel root saponin(e VI in the ethyl alcohol soaked extracting solution of various concentration detects figure.
Fig. 5 is the concentration quantitative figure of teasel root saponin(e VI in the ethyl alcohol soaked extracting solution of various concentration.
Fig. 6 is the chromatography detection figure that 60% ethyl alcohol impregnates teasel root saponin(e VI in different time extracting solution.
Fig. 7 is the concentration quantitative figure that 60% ethyl alcohol impregnates teasel root saponin(e VI in different time extracting solution.
Fig. 8 is the thin-layer chromatography colour developing figure that column extracts each section of MV.
Fig. 9 is the liquid chromatographic detection figure that column extracts each section of MV.
Figure 10 is the liquid chromatographic detection recovery rate quantitative statistics figure for repeating experiment more than three times that column extracts each section of MV.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
This embodiment offers the highly effective extraction methods of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 2.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 20ml under the conditions of temperature is 60% mixes, every 15min
It shakes up once, after impregnating 2h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 3000rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as soaked extracting solution containing teasel root saponin(e VI are taken.
Embodiment 2
This embodiment offers the highly effective extraction methods of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 2.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 20ml under the conditions of temperature is 60% mixes, every 15min
It shakes up once, after impregnating 3h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 3000rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as soaked extracting solution containing teasel root saponin(e VI are taken.
Embodiment 3
This embodiment offers the highly effective extraction methods of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 2.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 20ml under the conditions of temperature is 60% mixes, every 15min
It shakes up once, after impregnating 4h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 3000rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as soaked extracting solution containing teasel root saponin(e VI are taken.
Embodiment 4
This embodiment offers the highly effective extraction methods of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 1.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 10ml under the conditions of temperature is 40% mixes, every 15min
It shakes up once, after impregnating 4h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 4500rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as soaked extracting solution containing teasel root saponin(e VI are taken.
Embodiment 5
This embodiment offers the highly effective extraction methods of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 1.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 10ml under the conditions of temperature is 60% mixes, every 15min
It shakes up once, after impregnating 4h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 4500rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as soaked extracting solution containing teasel root saponin(e VI are taken.
Embodiment 6
This embodiment offers the highly effective extraction methods of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 1.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 10ml under the conditions of temperature is 80% mixes, every 15min
It shakes up once, after impregnating 4h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 4500rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as soaked extracting solution containing teasel root saponin(e VI are taken.
Embodiment 7
This embodiment offers the highly effective extraction methods of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, the above-mentioned Himalayan Teasel Root dress column of 8g is taken, is obtained
The column for being used for teasel root saponin(e VI to the teasel root column that internal diameter 1.8cm, dress pillar height degree are 9cm extracts, and under the conditions of 25 DEG C of temperature, uses
The Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration of 80ml is 60% mixes impregnates 3h, is subsequently placed in elution as solvent
Flow velocity is that 2BV/h carries out column extraction；Wherein, the maximum saturation liquid absorption amount volume mass ratio of Himalayan Teasel Root is 2.2,1MV=
(MV, minimum volume, minimum solvent volume are equal to and use ethyl alcohol and water mixed solution continuous as solvent extraction 8g 17.6ml
When teasel root saponin(e VI in disconnected powder, the volume of the maximum saturation lyosoption of Himalayan Teasel Root)；In addition, Fractional Collections
The column extracting solution containing teasel root saponin(e VI can be obtained in the extracting solution of five part 1MV, 2MV, 3MV, 4MV, 5MV.
Comparative example 1
The comparative example provides the highly effective extraction method of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 2.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 20ml under the conditions of temperature is 60% mixes, every 15min
It shakes up once, after impregnating 1h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 3000rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as extracting solution containing teasel root saponin(e VI are taken.
Comparative example 2
The comparative example provides the highly effective extraction method of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 1.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 10ml under the conditions of temperature is 20% mixes, every 15min
It shakes up once, after impregnating 4h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 4500rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as extracting solution containing teasel root saponin(e VI are taken.
Comparative example 3
The comparative example provides the highly effective extraction method of teasel root saponin(e VI a kind of, specific steps are as follows: firstly, taking teasel root
After medicinal material is crushed, it is sieved with 20-60 mesh screen, obtains Himalayan Teasel Root；Then, take the above-mentioned Himalayan Teasel Root of 1.0g at 25 DEG C
In the Extraction solvent that the second alcohol and water that the ethyl alcohol volumetric concentration for being immersed in 10ml under the conditions of temperature is 95% mixes, every 15min
It shakes up once, after impregnating 4h, obtains soak；Then above-mentioned soak is placed in the centrifuge that revolving speed is 4500rpm and is carried out
After being centrifugated 10min, supernatant liquor, the as extracting solution containing teasel root saponin(e VI are taken.
By mentioning for the part 1MV, 2MV, 3MV, 4MV, 5MV that the obtained extracting solution of above-described embodiment 1-6 and embodiment 7 obtain
The soaked extracting solution for taking liquid and comparative example 1-3 to obtain carries out content detection analysis according to following detection methods:
(1) the teasel root saponin(e VI solution of 5mg/mL the preparation of standard items stock solution: is prepared using methanol as solvent as mentioning
Take the standard items stock solution of teasel root saponin(e VI content detection in liquid；
(2) thin-layer chromatography detects: taking teasel root saponin(e VI soaked extracting solution, column extracting solution and standard items stock solution respectively in silicon
It is 4:1:5's with volume ratio after carrying out point sample (standard items are laid under liquid spotting 20, are extracted under liquid spotting 30) on glue G lamellae
N-butanol, acetic acid and water are developed the color (such as Fig. 1) as solvent, and with iodine vapor；
(3) the HPLC identification of standard items stock solution: using the teasel root saponin(e VI standard items stock solution of above-mentioned preparation as detection
Standard items, chromatographic condition are as follows: chromatographic column is Eclipse plusC18, and it is the acetonitrile and water of 25:75 that mobile phase, which selects volume ratio,
Detection time is 30min, Detection wavelength 212nm, flow velocity 1.0mL/min, and sample volume is 10 μ L；
(4) teasel root saponin(e VI linear relationship is investigated: the teasel root saponin(e VI standard items stock solution of above-mentioned preparation is diluted respectively
To the standard solution of following concentration gradient: 0.5mg/mL, 1mg/mL, 1.5mg/mL, 2mg/mL, 2.5mg/mL；Then distinguish
It draws 10 μ L and carries out HPLC detection, linear regression (such as attached drawing 2-3) is carried out to its concentration with the peak area of teasel root saponin(e VI,
According to the area at the obtained peak of extracting solution, the content of teasel root saponin(e VI ingredient in extracting solution is calculated.
The soak extraction that the obtained soaked extracting solution of embodiment 1-6 and comparative example 1-3 are obtained according to above-mentioned detection method
Liquid carries out result data (concentration of teasel root saponin(e VI in the extracting solution obtained in teasel root Chinese medicine) such as following table after content detection
1:
Table 1
In addition, according to above-mentioned detection method to the extracting solution of the part 1MV, 2MV, 3MV, 4MV, 5MV obtained in embodiment 7
Content analysis is carried out, (recovery rate: teasel root saponin(e VI accounts for its total amount in each extraction section of extracting solution for the result such as the following table 2 of analysis
Percentage):
Table 2
| Embodiment 7 | Recovery rate (%) |
| 1MV | 76.00±4.07 |
| 2MV | 15.61±2.21 |
| 3MV | 6.18±2.81 |
| 4MV | 1.88±0.71 |
| 5MV | 0.97±0.43 |
In conclusion the test result of embodiment 1-3 and comparative example 1 can see from upper table 1, Himalayan Teasel Root is being extracted
Soaking time in solvent will also result in certain influence to the recovery rate of teasel root saponin(e VI；Wherein, when the immersion of Himalayan Teasel Root
Between it is longer, the recovery rate of teasel root saponin(e VI is higher；But, when soaking time is greater than 3h, the recovery rate of teasel root saponin(e VI is improved
It is unobvious, therefore consider extraction time cost factor, extraction soaking time of the preferred 3h of the present invention as teasel root saponin(e VI.
The test result of embodiment 4-6 and comparative example 2-3 can see from table 1, use the ethyl alcohol of different volumes concentration
Teasel root saponin(e VI in teasel root is extracted with the Extraction solvent of water mixing, the recovery rate of teasel root saponin(e VI differs greatly；Moreover,
The concentration of ethyl alcohol is not in a linear relationship in the Extraction solvent of recovery rate and second the alcohol and water mixing of teasel root saponin(e VI.Wherein,
When the volumetric concentration of the Extraction solvent of second alcohol and water mixing is 60%, the recovery rate of teasel root saponin(e VI is higher.
In addition, can see from the different test results for extracting section of embodiment 7 in upper table 1, according to provided by the invention
Technical solution collects the first two MV, the extracted amount of teasel root saponin(e VI can be made to reach teasel root saponin(e VI total amount in teasel root
91.6%, so as to greatly reduce the usage amount of Extraction solvent.
Taking the above-mentioned ideal embodiment according to the present invention as inspiration, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property range is not limited to the contents of the specification, it is necessary to which the technical scope thereof is determined according to the scope of the claim.
Claims (7)
1. a kind of highly effective extraction method of teasel root saponin(e VI, using teasel root medicinal material as raw material, which comprises the following steps:
(1) after taking teasel root medicinal material to be crushed, sieving obtains Himalayan Teasel Root；
(2) above-mentioned Himalayan Teasel Root is taken to be immersed in the Extraction solvent of second alcohol and water mixing；
(3) Himalayan Teasel Root of above-mentioned immersion is used into column extraction method, the extracting solution containing teasel root saponin(e VI can be obtained；
The volumetric concentration of ethyl alcohol is 40%-80% in the Extraction solvent that second alcohol and water mixes in the step (2).
2. the highly effective extraction method of teasel root saponin(e VI according to claim 1 a kind of, which is characterized in that the step (1)
The mesh number of middle sieving is 20-60 mesh.
3. the highly effective extraction method of teasel root saponin(e VI according to claim 1 a kind of, which is characterized in that the step (2)
The time of middle immersion is 2-4h, and the temperature of immersion is 20-30 DEG C.
4. the highly effective extraction method of teasel root saponin(e VI according to claim 3 a kind of, which is characterized in that the step (2)
The time of middle immersion is 3h.
5. the highly effective extraction method of teasel root saponin(e VI according to claim 1 a kind of, which is characterized in that the second alcohol and water
The volumetric concentration of ethyl alcohol is 60% in mixed Extraction solvent.
6. a kind of highly effective extraction method of teasel root saponin(e VI according to claim 1 or 5, which is characterized in that the ethyl alcohol
Concentration with Himalayan Teasel Root in the Extraction solvent of water mixing is 0.08-0.12g/mL.
7. the highly effective extraction method of teasel root saponin(e VI according to claim 1 a kind of, which is characterized in that the step (3)
Center pillar extraction method comprises the concrete steps that: it is 1-3BV/h, diameter that the Extraction solvent that above-mentioned second alcohol and water mixes, which is placed in eluent flow rate,
Height is than extracting teasel root saponin(e VI in the Himalayan Teasel Root column for 1:(4-6), and 2-4 MV extracting solution simultaneously merges one before collecting
After rising, the extracting solution containing teasel root saponin(e VI can be obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811627297.9A CN109575101A (en) | 2018-12-28 | 2018-12-28 | A kind of highly effective extraction method of teasel root saponin(e VI |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811627297.9A CN109575101A (en) | 2018-12-28 | 2018-12-28 | A kind of highly effective extraction method of teasel root saponin(e VI |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109575101A true CN109575101A (en) | 2019-04-05 |
Family
ID=65933436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811627297.9A Pending CN109575101A (en) | 2018-12-28 | 2018-12-28 | A kind of highly effective extraction method of teasel root saponin(e VI |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109575101A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113336810A (en) * | 2021-01-15 | 2021-09-03 | 广州博济医药生物技术股份有限公司 | Method for extracting and purifying teasel root saponin VI with high bioavailability |
| CN114853840A (en) * | 2022-06-09 | 2022-08-05 | 中日友好医院(中日友好临床医学研究所) | Preparation method and application of akebia saponin D bulk drug |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366721A (en) * | 2008-08-25 | 2009-02-18 | 马仁强 | Bulk medicament for treating orthopedic disorders and preparation method thereof |
| CN101637474A (en) * | 2008-07-31 | 2010-02-03 | 山东绿叶天然药物研究开发有限公司 | New application of asperosaponin VI |
| CN101643488A (en) * | 2009-08-28 | 2010-02-10 | 重庆市中药研究院 | Extraction purification process of teasel root saponin |
| CN102180937A (en) * | 2011-03-28 | 2011-09-14 | 江苏神华药业有限公司 | Method for preparing enriched and refined akebin D with macroporous absorption resin |
| CN102526134A (en) * | 2010-12-24 | 2012-07-04 | 苏州宝泽堂医药科技有限公司 | Preparation method of dipsacus total saponins and teasel saponins VI |
| CN102924545A (en) * | 2012-11-13 | 2013-02-13 | 杨中林 | Enrichment and purifying method of akebiaquinata saponin D |
| CN103622983A (en) * | 2013-11-18 | 2014-03-12 | 滨州医学院 | Application of radix dipsaci saponin X in preparation of medicines for preventing or treating pulmonary fibrosis |
| KR101672841B1 (en) * | 2015-06-26 | 2016-11-04 | 주식회사 엘지생활건강 | Composition for improving skin |
-
2018
- 2018-12-28 CN CN201811627297.9A patent/CN109575101A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101637474A (en) * | 2008-07-31 | 2010-02-03 | 山东绿叶天然药物研究开发有限公司 | New application of asperosaponin VI |
| CN101366721A (en) * | 2008-08-25 | 2009-02-18 | 马仁强 | Bulk medicament for treating orthopedic disorders and preparation method thereof |
| CN101643488A (en) * | 2009-08-28 | 2010-02-10 | 重庆市中药研究院 | Extraction purification process of teasel root saponin |
| CN102526134A (en) * | 2010-12-24 | 2012-07-04 | 苏州宝泽堂医药科技有限公司 | Preparation method of dipsacus total saponins and teasel saponins VI |
| CN102180937A (en) * | 2011-03-28 | 2011-09-14 | 江苏神华药业有限公司 | Method for preparing enriched and refined akebin D with macroporous absorption resin |
| CN102924545A (en) * | 2012-11-13 | 2013-02-13 | 杨中林 | Enrichment and purifying method of akebiaquinata saponin D |
| CN103622983A (en) * | 2013-11-18 | 2014-03-12 | 滨州医学院 | Application of radix dipsaci saponin X in preparation of medicines for preventing or treating pulmonary fibrosis |
| KR101672841B1 (en) * | 2015-06-26 | 2016-11-04 | 주식회사 엘지생활건강 | Composition for improving skin |
Non-Patent Citations (1)
| Title |
|---|
| 朱海琳等: "《正交试验优选续断的水提工艺》", 《中国实验方剂学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113336810A (en) * | 2021-01-15 | 2021-09-03 | 广州博济医药生物技术股份有限公司 | Method for extracting and purifying teasel root saponin VI with high bioavailability |
| CN113336810B (en) * | 2021-01-15 | 2023-06-02 | 博济医药科技股份有限公司 | Extraction and purification method of dipsacus saponin VI with high bioavailability |
| CN114853840A (en) * | 2022-06-09 | 2022-08-05 | 中日友好医院(中日友好临床医学研究所) | Preparation method and application of akebia saponin D bulk drug |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109575101A (en) | A kind of highly effective extraction method of teasel root saponin(e VI | |
| CN105859803B (en) | A kind of preparation method of galloyl glucose | |
| CN108042590B (en) | Low-pesticide-residue ginseng stem and leaf extract and preparation method thereof | |
| CN105669631B (en) | A kind of potentilla plants extract and the method for therefrom separating four kinds of tanninses compounds | |
| CN110437059A (en) | A method of extraction prepares pachymic acid A and pachymic acid B from fuling peel | |
| CN106632546A (en) | Method for preparing two chemical reference substances of Rhoifolin and naringin simultaneously | |
| CN106957310B (en) | The high efficiency preparation method of flavonoids monomer in a kind of leaves of Hawthorn | |
| CN104402845B (en) | A kind of method utilizing ultrasonic wave to extract taxol from Chinese yew | |
| CN109528810A (en) | A method of there is the Chinese medical extract of antitumor action using high-speed countercurrent chromatography preparation | |
| CN109400665B (en) | Method for preparing four triterpenoid compound reference substances from pubescent holly root | |
| CN101176745A (en) | Method for making chinese medicine radix astragali | |
| CN104803838A (en) | Hedyotis caudatifolia anthraquinone compound and preparation method thereof | |
| CN106831936B (en) | The method that tanshinone IIA and dihydrotanshinone I are prepared using middle high-pressure preparation liquid phase method | |
| CN107286172B (en) | Aporphine alkaloid Illigerine B and its preparation method and application | |
| CN105085453B (en) | A kind of utilization high-speed countercurrent chromatography method that separation prepares oligomeric stilbene compound from Chinese small iris | |
| CN106831892A (en) | The preparation method of flavones monomer in a kind of leaves of Hawthorn | |
| CN106957309A (en) | The preparation method of lignanoids monomer in a kind of justicia | |
| CN108191933B (en) | Method for preparing new astilbin by taking rhizoma smilacis glabrae as raw material | |
| CN110204588A (en) | A method of preparing Astragaloside IV | |
| CN110187032A (en) | The measuring method of Pinoresinol diglucoside content in a kind of Eucommia ulmoides | |
| CN110015959A (en) | A method of efficiently separating purifying caffeoyl quinic acid isomers from mulberry leaf | |
| CN104447720A (en) | Method for separating vicenin-2 from linearstripe rabdosia herb | |
| CN104478878B (en) | A kind of method that separation from green peel of walnut prepares alkaloid | |
| CN108191922A (en) | Benzyl alcohol glycosides compound and preparation method thereof in coloquintida | |
| CN112552150B (en) | Method for preparing asarone monomer based on coordination effect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |