CN109548940A - 一种莓茶提取物的制备方法、产品及其应用 - Google Patents
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
本发明提供一种莓茶提取物的制备方法、产品及其应用,制备方法包括:将莓茶鲜叶摊凉后杀青,然后倒出揉捻机继续杀青至鲜叶完毕止;将杀青熟料装于揉捻机中,揉捻至有叶汁溢出物料表面;将揉捻成团的物料松开散热,用棉布打包,在32‑50℃下发酵1‑2h;将发酵后的湿热物料散开,干燥,得莓茶原茶;将莓茶原茶置于振荡筛震动,过120目筛分,得莓茶提取物。本发明生产工艺简单、环保,制备方法节能降耗,生产成本大幅降低,产品可用于制备具有抗氧化、增强免疫力、降血脂、护肝、清咽等作用的保健品。
Description
技术领域
本发明涉及植物提取物应用技术领域,具体涉及一种莓茶植物提取物的制备方法、产品及其应用。
背景技术
莓茶,又名土家霉茶、茅岩莓茶,系湘西北地区土家族先民,采用传统古法加工制成的一种养生保健代用茶,已有700多年的饮用历史,长期服用具有清热解毒,消炎利咽,止咳祛痰,降压减脂,保肝护肝,消除疲劳,抗氧化清除自由基,增强免疫力等多种功效。 加工莓茶的原植物,是葡萄科蛇葡萄属的一种藤本植物显齿蛇葡萄(Ampelopsis grossedentata)。文献报道,野生蛇葡萄属藤本植物我国已发现的有尖齿蛇葡萄、蓝果蛇葡萄、广东蛇葡萄、羽叶蛇葡萄、三裂蛇葡萄、贡山蛇葡萄、显齿蛇葡萄、大叶蛇葡萄等17种,每种依形态特征又有许多变种和生态类型,其天然产物的成分与组分及经济价值均有较大区别。传统医学及现代研究表明,蛇葡萄属中的显齿蛇葡萄,其有效部位茎叶干物质中总黄酮含量达6%以上,作为土家族常用的民族药物,收录于《土家族医药学》、《全国中草药汇编》、《湖南中药材(2009版)》等中医药专著中。2013年国家卫生和计划生育委员会发布第16号公告,批准显齿蛇葡萄叶为新食品原料,成为新的药食两用植物。显齿蛇葡萄其资源开发应用,市场前景广阔。
目前,莓茶生产上存在着原茶饮用不便、加工不便、产品附加值低的问题。以显齿蛇葡萄藤尖茎叶加工的干燥莓茶成品,藤茎纤细缠绕,体积蓬松硕大,包装运输成本高。原茶若直接饮用,泡茶时需手撕拉扯,既不卫生,又难把控茶汤比例,不易获得最佳口感效果。定量预包装小袋分装时,原茶破碎率高,粉末多,物料损耗高达20%以上;且加工技术含量低,产品单一,产业链短,制约了资源深度开发与产业延伸发展。
植物提取物具有目标成分含量高,产品质量稳定可控,便于包装运输等特点,可延伸开发成药品、食品、保健品、化妆品等多类别制成品,是植物资源深度开发的重要途径。目前,已有显齿蛇葡萄提取物上市并应用于制药行业,但生产工艺多以乙醇等溶剂提取为主,功效成分保存种类少,物料、能源及水资源消耗大,生产成本居高不下。
发明内容
本发明的目的在于提供一种莓茶提取物的制备方法,其采用固体制备方法,可保存和提高原植物中黄酮、多糖等大类功效成分含量,节能降耗,提高资源利用率,提高产品开发的多途径利用价值。
为达到上述目的,本发明的实施方案为:一种莓茶提取物的制备方法,包括:
(1)采摘莓茶鲜叶,摊凉后在260-280℃杀青1-2min,然后将物料放入揉捻机,继续杀青至鲜叶完毕止,得杀青熟料;
(2)将杀青熟料装于揉捻机中,开机揉捻3-5分钟,至有叶汁溢出物料表面时止;
(3)将揉捻成团的物料松开,散热后用棉布包紧,在32-50℃下发酵1-2h;
(4)将发酵后的湿热物料散开,干燥,得到析出晶体的白色莓茶原茶;
(5)将莓茶原茶置于振荡筛震动,过120目筛分,得莓茶提取物。
作为优化,步骤(1)中,所述莓茶鲜叶采收自7-9月,采摘部位为藤尖,藤尖长5-8厘米,莓茶鲜叶采后降温摊凉至20-25℃。
作为优化,步骤(1)中,所述杀青温度杀青温度265℃,杀青时间70-90秒钟。
作为优化,步骤(3)中,所述发酵时间90分钟,初始温度33℃,终止温度48℃。
作为优化,步骤(4)中,所述干燥方法是,先将散开的湿热物料细密网筛上,日晒4-8小时,至七到八成干,然后放于干燥箱,在温度60-90℃干燥时间1-2小时,至含水量3-5%。
本发明还提供一种如上述方法制备的莓茶提取物产品。该产品可用于制备具有抗氧化、增强免疫力、降血脂、护肝等作用的保健品。
本发明以莓茶鲜叶为原料,通过杀青、揉捻、发酵、析晶、日晒、干燥、取粉、分筛等步骤,得到目标产品莓茶提取物,可保留二氢杨梅素、杨梅素、酸多糖等多种药用有效成分,作为多种大健康产品的基础原料。本发明与常规的植物提取溶剂法或水提法生产工艺相比,不使用大型植物提取、分离、纯化、喷雾干燥等设备,不使用乙醇或水等其他液体作溶媒,即可得到较高纯度的植物提取物。生产工艺简单、环保,制备方法节能降耗,生产成本大幅降低,产品用途更为广泛。本发明得到的莓茶提取物,总黄酮含量可达到45-65%,二氢杨梅素含量34-52%,杨梅素含量≧1%,多糖含量≧2%。经功效评价动物模型试验,证实具有抗氧化、增强免疫力、降血脂、护肝、清咽等多重功效。
具体实施方式
下面给出本发明的优选实施案例,以详细说明本发明。
实施例1:7月中旬采摘张家界市永定区罗塔坪乡四关峪村人工种植的地产莓茶鲜叶(经鉴别为显齿蛇葡萄小叶种)100公斤,摊凉后投入滚筒式杀青机高温杀青,每次投鲜叶10公斤,杀青温度265℃,杀青时间70-90秒钟;停机将物料倒出放入揉捻机,继续投料杀青至鲜叶完毕止,得杀青熟料89.5公斤;将熟料分装于2台揉捻机,开机揉捻3分钟,至有叶汁溢出物料表面时止,松开成团物料散热15分钟,用白棉布包紧打包发酵,发酵时间90分钟,初始温度33℃,终止温度48℃,散开发酵包可见物料上有白色结晶析出;将湿热物料散开摊于细密网筛上,置晒架上日晒4小时,物料变白后放入热风循环干燥箱,设定干燥温度90℃,干燥时间2小时,干燥后得莓茶原茶23.48公斤;将原茶置于振荡筛震动,过120目筛分,得结晶粉末5.24公斤,鲜叶莓茶提取物得率5.24%。经取样,用高效液相色谱法检测,样品总黄酮含量(以二氢杨梅素计)62.4%,二氢杨梅素含量49.2%,杨梅素含量1.2%;用苯酚硫酸比色法检测样品多糖含量(以葡萄糖为对照品)2.2%。
实施例2:2018年9月24日采摘张家界市永定区罗塔坪乡四关峪村人工种植的地产莓茶鲜叶100公斤,摊凉后投入滚筒式杀青机高温杀青,每次投鲜叶10公斤,杀青温度275℃,杀青时间80-90秒钟;停机将物料倒出放入揉捻机,继续投料杀青至鲜叶完毕止,得杀青熟料94.2公斤;将熟料分装于2台揉捻机,开机揉捻,3分钟,至有叶汁溢出物料表面时止,松开成团物料散热10分钟,用白棉布包紧打包发酵,发酵时间90分钟,初始温度32℃,终止温度45℃,散开发酵包可见物料上有白色结晶析出;将湿热物料散开摊于细密网筛上,置晒架上日晒7小时,物料变白后放入热风循环干燥箱,设定干燥温度2小时,干燥后得莓茶原茶25.75公斤。将原茶置于振荡筛震动,过120目筛分,得结晶粉末5.85公斤,鲜叶莓茶提取物得率5.85%。经取样,用高效液相色谱法检测,样品总黄酮含量(以二氢杨梅素计)53.6%,二氢杨梅素含量40.4%,杨梅素含量1.4%;用苯酚硫酸比色法检测样品多糖含量(以葡萄糖为对照品)2.5%。
实施例3:用以上实施例制备的莓茶提取物,在湖南动物实验中心,按卫生部2003版保健食品功效评价指导意见标准方法做动物模型试验,主要试验结果与结论如下:
(1)莓茶提取物对抗氧化功能的影响
雄性SD大鼠40只,体重180.1~210.6g,按体重随机分为模型对照组、莓茶提取物低、中、高剂量(0.215g/kg、0.43g/kg、0.86g/kg),每组10只,各组每天灌胃给予不同药液,给药体积为10mL/kg,1次/日,连续30天,模型对照组同等体积的蒸馏水。末次灌胃后,各组禁食16h(过夜),然后1次性灌胃给予50%乙醇12 mL/kg•bw,6h后麻醉,腹主动脉采血检测血清脂质氧化产物(MDA)、抗氧化活力(SOD)、谷胱甘肽(GSH)、还原性谷胱甘肽(GSH-PX)含量。试验结果表明,莓茶提取物能明显升高血清SOD、MDA、GSH-PX水平。
(2)莓茶提取物对化学性肝损伤的影响
雄性ICR小鼠50只,体重18.2~22.2g,按体重随机分为正常对照组、模型对照组、莓茶水提物低、中、高剂量(0.43g/kg、0.86g/kg、1.72g/kg),每组10只,各组每天灌胃给予不同药液,给药体积为20mL/kg,1次/日,连续30天,正常对照组和模型对照组灌胃给予等体积的蒸馏水。于给药第29d将各组动物禁食不禁水16h,模型对照组及各组小鼠按5mL/kg,灌胃给予1.0%的CCl4植物油溶液(折合CCl4的剂量为80mg/kg.bw),正常对照组按相同方法灌胃给予植物油,除正常对照组外,其余各组给予CCl4后4h,各组小鼠继续灌胃给予相应药液,24h后眼眶后静脉丛取血,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平以及肝脏组织病理学检查。试验结果表明,莓茶提取物中、高剂量能明显降低小鼠血清ALT活性;低、中、高剂量能明显降低小鼠血清AST活性。
(3)莓茶提取物对血脂的影响
雄性SD大鼠40只,体重180.3~220.5g,取10只大鼠作为正常对照组,其余大鼠饲喂给予高脂饲料(60%维持饲料、20%蔗糖、15%猪油、1.2%胆固醇、0.2胆酸钠),饲喂2周后,各组大鼠禁食不禁水12h,次日大鼠眼眶后静脉丛采血,检测血清CHO、TG、LDL-L、HDL-L水平。根据CHO指标将模型大鼠随机分为4组,分别为:模型对照组、莓茶水提物低、中、高剂量(0.215g/kg、0.43g/kg、0.86g/kg),每组10只,各组每天灌胃给予不同药液,给药体积为10mL/kg,1次/日,连续30天,正常对照组和模型对照组动物灌胃给予等体积的蒸馏水。末次灌胃后,各组组禁食16h,麻醉,腹主动脉采血检测血清CHO、TG、LDL-L、HDL-L含量。试验结果表明,莓茶水提取物高剂量能明显降低血清TG水平、升高HDL-L水平。
(4)莓茶水提物对调节免疫功能的影响
①小鼠抗体生成细胞的影响试验:雄性BALB/c小鼠50只,体重18.5~21.4g,按体重随机分为正常对照组、莓茶水提物低、中、高剂量组(0.43g/kg、0.86g/kg、1.72g/kg),每组10只,除正常对照组灌胃给予蒸馏水外,其余各组分别灌胃给予相应药液,灌胃体积为20ml/kg,每日1次,连续30天。各组D19、D21、D23、D26、D28、D30天小鼠腹腔注射SRBC悬液0.2mL/只进行免疫,连续免疫6天。 免疫6天后,将小鼠颈椎脱臼处死,取出脾脏,制备脾细胞悬液加入0.2%SRBC、补体温育1h,3000rpm离心取上清液采用酶标仪在波长570nm测定OD值。
②小鼠碳粒廓清的影响试验:雄性BALB/c小鼠40只,体重18.3~22.0g,按体重随机分为正常对照组、莓茶水提物低、中、高剂量(0.43g/kg、0.86g/kg、1.72g/kg)组,10只/组,除正常对照组灌胃给予蒸馏水外,其余各组分别灌胃给予相应药液,灌胃体积为20ml/kg,每天1次,连续30天。末次给药后按体重从小鼠尾静脉注入稀释的印度墨汁(10mL/kg),待墨汁注入,立即计时。注入墨汁后第2min、第10min.分别从内毗静脉丛取血20µL,并立即将其加到2mL 0.1%Na2CO3溶液中。用酶标仪在600nm波长处测OD值,以Na2CO3溶液作空白对照。将小鼠处死,取肝脏和脾脏,计算吞噬指数α。
实验结果:莓茶提取物中、高剂量能明显升高抗体细胞生成,能明显增加吞噬指数和吞噬系数。
综上述,莓茶提取物具有明显的抗氧化、保肝、降脂和调节免疫作用。
Claims (7)
1.一种莓茶提取物的制备方法,其特征在于,包括以下步骤:
(1)采摘莓茶鲜叶,摊凉后在260-280℃杀青1-2min,然后将物料放入揉捻机,继续杀青至鲜叶完毕止,得杀青熟料;
(2)将杀青熟料装于揉捻机中,开机揉捻3-5分钟,至有叶汁溢出物料表面时止;
(3)将揉捻成团的物料松开,散热后用棉布包紧,在32-50℃下发酵1-2h;
(4)将发酵后的湿热物料散开,干燥,得到析出晶体的白色莓茶原茶;
(5)将莓茶原茶置于振荡筛震动,过120目筛分,得莓茶提取物。
2.根据权利要求1所述的莓茶提取物的制备方法,其特征在于,步骤(1)中,所述莓茶鲜叶采收自7-9月,采摘部位为藤尖,藤尖长5-8厘米,莓茶鲜叶采后降温摊凉至20-25℃。
3.根据权利要求1所述的莓茶提取物的制备方法,其特征在于,步骤(1)中,所述杀青温度杀青温度265℃,杀青时间70-90秒钟。
4.根据权利要求1所述的莓茶提取物的制备方法,其特征在于,步骤(3)中,所述发酵时间90分钟,初始温度33℃,终止温度48℃。
5.根据权利要求1所述的莓茶提取物的制备方法,其特征在于,步骤(4)中,所述干燥方法是,先将散开的湿热物料细密网筛上,日晒4-8小时,至七到八成干,然后放于干燥箱,在温度60-90℃干燥时间1-2小时,至含水量3-5%。
6.一种如权利要求1所述的方法制备的莓茶提取物产品。
7.如权利要求6所述的莓茶提取物产品在制备具有抗氧化、增强免疫力、降血脂、护肝作用保健品方面的应用。
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