CN109517850A - The method for preparing sodium gluconate as raw material using raffinate after glucose crystallization - Google Patents
The method for preparing sodium gluconate as raw material using raffinate after glucose crystallization Download PDFInfo
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- CN109517850A CN109517850A CN201811166527.6A CN201811166527A CN109517850A CN 109517850 A CN109517850 A CN 109517850A CN 201811166527 A CN201811166527 A CN 201811166527A CN 109517850 A CN109517850 A CN 109517850A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
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Abstract
This application involves the work silver for preparing sodium gluconate, the method for specially preparing sodium gluconate as raw material using raffinate after glucose crystallization includes the following steps that (1) carries out decolorization to AD liquid, makes AD liquid form and aspect less than No. 1；(2) water is added to be made into the standard AD solution that mass concentration is 29-31% the AD liquid after decoloration；(3) standard AD solution is added in fermentor and glucose oxidase and catalase is added；(4) filtrated air is passed through with the speed of 0.2-1.5v/v/m into the fermentor of step (3), controlling the temperature in fermentor is 30-50 DEG C, with the pH of solution in sodium hydroxide solution adjusting fermentor between 5-7, and concentration of reduced sugar is stirred into mixed liquor with the speed of 100-150rpm and is reduced to 0.2% or less stopping reaction；(5) reaction solution condensing crystallizing obtained by step (4) is both obtained into crystal glucose acid sodium product.
Description
Technical field
This application involves the techniques for preparing gluconate, specially prepare Portugal by raw material of raffinate after glucose crystallization
The method of grape sodium saccharate.
Background technique
Sodium gluconate market price competition is fierce in recent years, traditional handicraft sodium gluconate with glucose solution or
Starch glucose saccharifying liquid is raw material, not only at high cost, and anti-as raw material production sodium gluconate using starch glucose saccharifying liquid
Answer protein content in liquid high, the post-processing link difficulty such as decoloration, crystallization is big.
Summary of the invention
In order to solve the above-mentioned technical problem, this application provides prepare glucose using raffinate after glucose crystallization as raw material
The method of sour sodium, the application are realized by following proposal:
It is the method that raw material prepares sodium gluconate with raffinate after glucose crystallization (abbreviation AD liquid), includes the following steps, (1)
Decolorization is carried out to AD liquid, makes AD liquid form and aspect less than No. 1；(2) adding water to be made into mass concentration the AD liquid after decoloration is 29-
31% standard AD solution；(3) standard AD solution is added in fermentor and glucose oxidase and catalase is added；(4)
Speed into the fermentor of step (3) with 0.2-1.5v/v/m is passed through filtrated air, and controlling the temperature in fermentor is 30-50
DEG C, it is stirred between 5-7, and with the speed of 100-150rpm to mixing with the pH that sodium hydroxide solution adjusts solution in fermentor
Concentration of reduced sugar is reduced to 0.2% or less stopping reaction in liquid；(5) reaction solution condensing crystallizing obtained by step (4) must both be crystallized into Portugal
Grape sodium saccharate product.
Preferably, decolorising agent used in decolorization is active carbon in the step (1), and the active carbon is AD liquid quality
Thousand/0.5- 1/1000th.
Preferably, in the step (1) the step of decolorization are as follows: decolorising agent is uniformly mixed with AD liquid, after in 60-65
15-39min is kept the temperature at DEG C, rear filtering removal decolorising agent.
Preferably, the additional amount of step (3) the Plays AD solution is the 2/3 of fermenter volume.
Preferably, in the step (3), the dosage of glucose oxidase is the 150% of theoretical enzyme activity amount, catalase
Dosage be the 150% of theoretical enzyme activity amount.
Preferably, the concentration of sodium hydroxide solution is 0.5-11mol/L in the step (4).
The beneficial effect of the application is: AD liquid is by-product cost after glucose crystallization relative to glucose and starch sugar
It is at low cost to change liquid；AD liquid phase is low for starch glucose saccharifying liquid protein content, to produce sodium gluconate, decoloration, crystallization
Equal products aftertreatment technology link is relatively easy to, thus at low cost right using present invention process production sodium gluconate production
It is low in the pollution of the environment.
Hue circle used herein is 12 hue circles.
Specific embodiment
Embodiment 1
In the method that raffinate after glucose crystallization prepares sodium gluconate as raw material, include the following steps, (1) carries out AD liquid
Decolorization makes AD liquid form and aspect less than No. 1；(2) water is added to be made into the standard AD that mass concentration is 29-31% the AD liquid after decoloration
Solution；(3) standard AD solution is added in fermentor and glucose oxidase and catalase is added；(4) to step (3)
Speed in fermentor with 0.2-1.5v/v/m is passed through filtrated air, and controlling the temperature in fermentor is 30-50 DEG C, uses hydroxide
The pH that sodium solution adjusts solution in fermentor stirs into mixed liquor reduced sugar between 5-7, and with the speed of 100-150rpm
Concentration is reduced to 0.2% hereinafter, stopping reaction；(5) reaction solution condensing crystallizing obtained by step (4) is both obtained into crystal glucose acid sodium
Product；
Enzyme activity amount is the micromole's number for changing substrate in 1ml enzyme one minute to product；
0.2v/v/m is passed through the filtrated air of 0.2 times of volume of reaction solution volume per minute.
Embodiment 2
On the basis of embodiment 1, decolorising agent used in decolorization is active carbon in the step (1), and the active carbon is
Thousand/0.5- 1/1000th of AD liquid quality.
Embodiment 3
On the basis of embodiment 2, in the step (1) the step of decolorization are as follows: decolorising agent is uniformly mixed with AD liquid, after
15-39min is kept the temperature at 60-65 DEG C, rear filtering removal decolorising agent.
Embodiment 4
On the basis of embodiment 3, the additional amount of step (3) the Plays AD solution is the 2/3 of fermenter volume.
Embodiment 5
On the basis of embodiment 6, in the step (3), the dosage of glucose oxidase is the 150% of theoretical enzyme activity amount, peroxide
The dosage for changing hydrogen enzyme is the 150% of theoretical enzyme activity amount.
The ingredient of AD liquid and saccharified liquid comparison such as following table；
Embodiment 6
In the method that raffinate after glucose crystallization prepares sodium gluconate as raw material, include the following steps, (1) carries out AD liquid
Decolorization makes AD liquid form and aspect less than No. 1；(2) by the AD liquid after decoloration add water be made into mass concentration be 29% standard AD it is molten
Liquid；(3) standard AD solution is added in fermentor and glucose oxidase and catalase is added；(4) to the hair of step (3)
Speed in fermentation tank with 1.5v/v/m is passed through filtrated air, and controlling the temperature in fermentor is 30 DEG C, with sodium hydroxide solution tune
The pH of solution in fermentor is saved between 5-7, and concentration of reduced sugar is stirred into mixed liquor with the speed of 100rpm and is reduced to
0.2% or less stops reaction；(5) reaction solution condensing crystallizing obtained by step (4) is both obtained into crystal glucose acid sodium product；
Preferably, decolorising agent used in decolorization is active carbon in the step (1), and the active carbon is the thousand of AD liquid quality
/ 0.5；
Preferably, in the step (1) the step of decolorization are as follows: decolorising agent is uniformly mixed with AD liquid, after protected at 60 DEG C
Warm 15min, rear filtering removal decolorising agent；
Preferably, the additional amount of step (3) the Plays AD solution is the 2/3 of fermenter volume；
Preferably, in the step (3), the 150% of the theoretical enzyme activity amount of glucose oxidase, the theoretical enzyme activity of catalase
The 150% of amount.
Embodiment 7
In the method that raffinate after glucose crystallization prepares sodium gluconate as raw material, include the following steps, (1) carries out AD liquid
Decolorization makes AD liquid form and aspect less than No. 1；(2) by the AD liquid after decoloration add water be made into mass concentration be 31% standard AD it is molten
Liquid；(3) standard AD solution is added in fermentor and glucose oxidase and catalase is added；(4) to the hair of step (3)
Speed in fermentation tank with 0.2v/v/m is passed through filtrated air, and controlling the temperature in fermentor is 50 DEG C, with sodium hydroxide solution tune
The pH of solution in fermentor is saved between 5-7, and concentration of reduced sugar is stirred into mixed liquor with the speed of 150rpm and is reduced to
0.2% or less stops reaction；(5) reaction solution condensing crystallizing obtained by step (4) is both obtained into crystal glucose acid sodium product；
Preferably, decolorising agent used in decolorization is active carbon in the step (1), and the active carbon is the thousand of AD liquid quality
/ 1；
Preferably, in the step (1) the step of decolorization are as follows: decolorising agent is uniformly mixed with AD liquid, after protected at 65 DEG C
Warm 39min, rear filtering removal decolorising agent；
Preferably, the additional amount of step (3) the Plays AD solution is the 2/3 of fermenter volume；
Preferably, in the step (3), the 150% of the theoretical enzyme activity amount of glucose oxidase, the theoretical enzyme activity of catalase
The 150% of amount.
Embodiment 8
In the method that raffinate after glucose crystallization prepares sodium gluconate as raw material, include the following steps, (1) carries out AD liquid
Decolorization makes AD liquid form and aspect less than No. 1；(2) by the AD liquid after decoloration add water be made into mass concentration be 30% standard AD it is molten
Liquid；(3) standard AD solution is added in fermentor and glucose oxidase and catalase is added；(4) to the hair of step (3)
Speed in fermentation tank with 1.2v/v/m is passed through filtrated air, and controlling the temperature in fermentor is 40 DEG C, with sodium hydroxide solution tune
The pH of solution in fermentor is saved between 5-7, and concentration of reduced sugar is stirred into mixed liquor with the speed of 125rpm and is reduced to
0.2% or less stops reaction；(5) reaction solution condensing crystallizing obtained by step (4) is both obtained into crystal glucose acid sodium product；
Preferably, decolorising agent used in decolorization is active carbon in the step (1), and the active carbon is the thousand of AD liquid quality
/ 0.75；
Preferably, in the step (1) the step of decolorization are as follows: decolorising agent is uniformly mixed with AD liquid, after protected at 62 DEG C
Warm 22min, rear filtering removal decolorising agent；
Preferably, the additional amount of step (3) the Plays AD solution is the 2/3 of fermenter volume；
Preferably, in the step (3), the 150% of the theoretical enzyme activity amount of glucose oxidase, the theoretical enzyme activity of catalase
The 150% of amount.
Embodiment 9
On the basis of embodiment 8, mixing speed when decolorising agent is mixed with AD liquid is 1000r/min.
Embodiment 10
On the basis of embodiment 5, the concentration of sodium hydroxide solution is 0.5-11mol/L in the step (4).
Claims (6)
1. the method for preparing sodium gluconate as raw material using raffinate after glucose crystallization, which is characterized in that include the following steps,
(1) decolorization is carried out to AD liquid, makes AD liquid form and aspect less than No. 1；(2) add water to be made into mass concentration the AD liquid after decoloration to be
The standard AD solution of 29-31%；(3) standard AD solution is added in fermentor and glucose oxidase and catalase is added；
(4) speed into the fermentor of step (3) with 0.2-1.5v/v/m is passed through filtrated air, and the temperature controlled in fermentor is
It 30-50 DEG C, is stirred between 5-7, and with the speed of 100-150rpm with the pH that sodium hydroxide solution adjusts solution in fermentor
Into mixed liquor, concentration of reduced sugar is reduced to 0.2% or less stopping reaction；(5) reaction solution condensing crystallizing obtained by step (4) had both been obtained
Crystal glucose acid sodium product.
2. the method for preparing sodium gluconate as raw material using raffinate after glucose crystallization as described in claim 1, feature
Be, in the step (1) decolorising agent used in decolorization be active carbon, the active carbon be AD liquid quality thousand/
0.5- 1/1000th.
3. the method for preparing sodium gluconate as raw material using raffinate after glucose crystallization as described in claim 1, feature
Be, in the step (1) the step of decolorization are as follows: decolorising agent is uniformly mixed with AD liquid, after kept the temperature at 60-65 DEG C
15-39min, rear filtering removal decolorising agent.
4. the method for preparing sodium gluconate as raw material using raffinate after glucose crystallization as described in claim 1, feature
It is, the additional amount of step (3) the Plays AD solution is the 2/3 of fermenter volume.
5. the method for preparing sodium gluconate as raw material using raffinate after glucose crystallization as described in claim 1, feature
It is, in the step (3), the dosage of glucose oxidase is the 150% of theoretical enzyme activity amount, and the dosage of catalase is reason
By the 150% of enzyme activity amount.
6. the method for preparing sodium gluconate as raw material using raffinate after glucose crystallization as described in claim 1, feature
It is, the concentration of sodium hydroxide solution is 0.5-11mol/L in the step (4).
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| CN201811166527.6A CN109517850A (en) | 2018-10-08 | 2018-10-08 | The method for preparing sodium gluconate as raw material using raffinate after glucose crystallization |
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| CN201811166527.6A CN109517850A (en) | 2018-10-08 | 2018-10-08 | The method for preparing sodium gluconate as raw material using raffinate after glucose crystallization |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7166200A (en) * | 1995-12-27 | 2001-03-08 | Genencor International, Inc. | Process for the preparation of gluconic acid and gluconic acid produced thereby |
| CN102102115A (en) * | 2010-12-09 | 2011-06-22 | 山东西王生化科技有限公司 | Method for preparing calcium gluconate and isomaltooligosaccharide simultaneously with crystalline glucose mother liquor |
| CN104450806A (en) * | 2014-11-27 | 2015-03-25 | 江南大学 | Method for preparing sodium gluconate with enzymatic method |
| CN106589011A (en) * | 2016-12-22 | 2017-04-26 | 浙江华康药业股份有限公司 | Processing method of xylose mother liquid |
-
2018
- 2018-10-08 CN CN201811166527.6A patent/CN109517850A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7166200A (en) * | 1995-12-27 | 2001-03-08 | Genencor International, Inc. | Process for the preparation of gluconic acid and gluconic acid produced thereby |
| CN102102115A (en) * | 2010-12-09 | 2011-06-22 | 山东西王生化科技有限公司 | Method for preparing calcium gluconate and isomaltooligosaccharide simultaneously with crystalline glucose mother liquor |
| CN104450806A (en) * | 2014-11-27 | 2015-03-25 | 江南大学 | Method for preparing sodium gluconate with enzymatic method |
| CN106589011A (en) * | 2016-12-22 | 2017-04-26 | 浙江华康药业股份有限公司 | Processing method of xylose mother liquid |
Non-Patent Citations (4)
| Title |
|---|
| 姜锡瑞等: "《生物发酵产业技术》", 31 May 2016, 中国轻工业出版社 * |
| 尤新: "《玉米深加工技术（第二版）》", 31 January 2008, 中国轻工业出版社 * |
| 陈建初等: "用葡萄糖母液制备混凝土高效缓凝剂", 《武汉工程大学学报》 * |
| 陈璥: "《玉米淀粉工业手册》", 30 September 2009, 中国轻工业出版社 * |
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