CN109496493A - A method of promote Chinese herbaceous peony bud to sprout - Google Patents
A method of promote Chinese herbaceous peony bud to sprout Download PDFInfo
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- CN109496493A CN109496493A CN201811504625.6A CN201811504625A CN109496493A CN 109496493 A CN109496493 A CN 109496493A CN 201811504625 A CN201811504625 A CN 201811504625A CN 109496493 A CN109496493 A CN 109496493A
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- cell division
- basic element
- herbaceous peony
- chinese herbaceous
- bud
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- 241000736199 Paeonia Species 0.000 title claims abstract description 53
- 235000006484 Paeonia officinalis Nutrition 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 49
- 239000003337 fertilizer Substances 0.000 claims abstract description 38
- 230000032823 cell division Effects 0.000 claims abstract description 35
- 230000002262 irrigation Effects 0.000 claims abstract description 22
- 238000003973 irrigation Methods 0.000 claims abstract description 22
- 150000001875 compounds Chemical class 0.000 claims abstract description 16
- 230000003442 weekly effect Effects 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- 239000006013 carbendazim Substances 0.000 claims description 8
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical group C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 8
- 210000002615 epidermis Anatomy 0.000 claims description 7
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 239000002689 soil Substances 0.000 claims description 7
- 235000020712 soy bean extract Nutrition 0.000 claims description 7
- 230000001737 promoting effect Effects 0.000 claims description 5
- 230000000855 fungicidal effect Effects 0.000 claims description 4
- 239000000417 fungicide Substances 0.000 claims description 4
- 238000012545 processing Methods 0.000 abstract description 10
- 241000196324 Embryophyta Species 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 241000219000 Populus Species 0.000 abstract description 6
- 241000205585 Aquilegia canadensis Species 0.000 abstract description 5
- 240000002853 Nelumbo nucifera Species 0.000 abstract description 5
- 235000006508 Nelumbo nucifera Nutrition 0.000 abstract description 5
- 235000006510 Nelumbo pentapetala Nutrition 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 2
- 206010052428 Wound Diseases 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 6
- 235000009161 Espostoa lanata Nutrition 0.000 description 5
- 240000001624 Espostoa lanata Species 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 241000921313 Phyllopodium Species 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to field of plant growing technology, more particularly to a kind of method that promotion Chinese herbaceous peony bud is sprouted, the described method includes: mid or late June per hectare applies 600~750kg compound fertilizer, pouring root is carried out with the basic element of cell division, the trickle irrigation basic element of cell division weekly later, trickle irrigation 2~5 times altogether, mid-August, per hectare applied 2250~3000kg cake fertilizer.The method of the present invention promotes Chinese herbaceous peony underground bud to sprout significant effect.Using the method for the present invention, odd liquid distilled from honeysuckle flowers or lotus leaves frost ' the Chinese herbaceous peony bud stem of four Nian Sheng ', which frequently compares, improves 67.7%, than being applied alone the processing of the 500mg/L basic element of cell division to improve 40.1%；Four Nian Sheng ' poplar prince wifes go out to bathe ' frequently control improves 88.2% to Chinese herbaceous peony bud stem, than being applied alone basic element of cell division processing to improve 38.9%.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a method of promote Chinese herbaceous peony bud to sprout.
Background technique
Chinese herbaceous peony is the perennial herb flowers of Paeoniaceae Paeonia, is that traditional garden ornamental plant and emerging cut-flower are planted
Object.Herbaceous peony kind is since female, stamen valve rate is high, forfeiture or some lost robustness, and seed propagation is not able to maintain sight
Character is appreciated, therefore generally uses division propagation.Chinese herbaceous peony division propagation has to band bud, divides root kind root that cannot sprout Chinese herbaceous peony on the ground merely
Part, so rare Cultivars of Chinese Herbaceous Peony is valuated with each bud.Bud germinating efficiency differs between Cultivars of Chinese Herbaceous Peony, but overall relatively low, general 1-2
Year, raw Chinese herbaceous peony only germinated 1-2 bud, and triennial Chinese herbaceous peony current year germinating 3-8 bud, raw Chinese herbaceous peony germinates 4-15 bud within 4 years.
The generation of plant sprout by plant itself conditional decision, also with external environment locating for plant and plant hormone in vivo
Level has close ties.Therefore often set about the generation how research promotes bud in production in terms of these.To external environment
The influence for promoting bud to occur is more with the research of illumination, temperature；Plant hormone then applies cytokinin in addition, gibberellin class is planted
The research of object growth regulator is more.
Xu Shilei has found in the research that Chinese herbaceous peony plant promotees bud: taking pouring root mode to perennial ' big rich and honour ', ' surprise flower
Dew frost ', ', poplar prince wife went out to bathe ' carry out 300mgL-1、600mg·L-1、900mg·L-1The basic element of cell division (the 6- of 3 gradients
Benzylaminopurine, 6-BA) processing, clear water processing is as control.The results showed that in terms of Chinese herbaceous peony bud stem ratio, 6-
BA is bigger between the rush bud effect difference 3 Cultivars of Chinese Herbaceous Peony, for ' big rich and honour ', 300mgL-1It is most bright that processing promotees bud effect
It is aobvious, it is more by 0.18 than compareing.For ' odd liquid distilled from honeysuckle flowers or lotus leaves frost ', the rush bud effect of the 6-BA of 600mgL-1 is the most obvious, more than compareing
1.18；For ' poplar prince wife goes out to bathe ', 900mgL-1Processing rush bud effect is the most obvious, and than compareing more 0.35, (Xu Shilei Chinese herbaceous peony is planted
Research [D] the Shandong Agricultural University of strain rush bud, 2012.).As it can be seen that Chinese herbaceous peony rush bud effect is obvious, to improve Chinese herbaceous peony reproductive efficiency,
It is necessary to further study Chinese herbaceous peony to promote bud method.
Summary of the invention
For what said above, the present invention provides a kind of method that promotion Chinese herbaceous peony bud is sprouted.
The invention adopts the following technical scheme:
First aspect of the present invention provides a kind of method that promotion Chinese herbaceous peony bud is sprouted, which comprises mid or late June
Per hectare applies 600~750kg compound fertilizer, carries out pouring root with the basic element of cell division, later the trickle irrigation basic element of cell division weekly, altogether trickle irrigation 2
~5 times, mid-August applies cake fertilizer.
Further, Suo Shi compound fertilizer N, P, K composition ratio: 10:20~40:15~20.Spraying the compound fertilizer can be fast
Speed supplement P, K fertilizer, promotes bud differentiation.
Further, pouring root is carried out with the basic element of cell division in early or mid July.Present invention discover that apply fertilizer in mid or late June, energy
Fertilizer efficiency is played to greatest extent, guarantees high-efficiency operation of the fertilizer to underground bud.It is the key that Chinese herbaceous peony bud differentiation at the beginning of 7 months by the end of June
Period, underground bud are transferred to generative growth phase by vegetative growth phase, i.e. phyllopodium is converted to sepal former base, are cut at this time with knife
Rhizome, and pouring root is carried out with the basic element of cell division, rhizome cell division can be rapidly promoted, the growth of bud is accelerated, promotes the conversion of bud.
Further, the basic element of cell division is total to trickle irrigation 3 times.
Further, with the method for basic element of cell division pouring root are as follows: pull down soil layer at Chinese herbaceous peony rhizome, scratch Chinese herbaceous peony rhizome with knife
The epidermis at place surrounds the slow trickle irrigation of rhizome portion with the basic element of cell division of 350~500mg/L, then sterilizes wound with fungicide, then
Earthing.
Further, the dosage of the basic element of cell division is 800~1200mL.
Further, the fungicide is carbendazim.
Further, the concentration of carbendazim is 800~1000 times of liquid.
Further, applied cake fertilizer be with soybean extract oil residue with the water mixed fermentation of 1:3 in mass ratio~5 is decomposed obtains,
30~40 times are diluted with water when use.
Further, per hectare applies 2250~3000kg cake fertilizer.Mid-August applies cake fertilizer, can be the life of Chinese herbaceous peony underground bud
Long development provides sufficient nutrition, guarantees that the later period development fertility of bud is sufficient.
It is that the present invention obtains the utility model has the advantages that
The method of the present invention promotes Chinese herbaceous peony bud to sprout significant effect.Using the method for the present invention, odd liquid distilled from honeysuckle flowers or lotus leaves frost ' the Chinese herbaceous peony bud of four Nian Sheng '
Stem frequently compares and improves 67.7%, than being applied alone the processing of the 500mg/L basic element of cell division to improve 40.1%；Four Nian Sheng ' poplar prince wifes go out to bathe ' Chinese herbaceous peony
Medicine bud stem frequently compares and improves 88.2%, than being applied alone basic element of cell division processing to improve 38.9%.
Specific embodiment
It is noted that described further below be all exemplary, it is intended to provide further instruction to the present invention.Unless another
It indicates, all technical and scientific terms used herein has usual with general technical staff of the technical field of the invention
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to exemplary embodiments of the present invention.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation and/or their combination.
In order to enable those skilled in the art can clearly understand technical solution of the present invention, below with reference to tool
The embodiment of the body technical solution that the present invention will be described in detail.
A kind of method for promoting Chinese herbaceous peony bud to sprout of embodiment 1
The method are as follows:
Mid or late June per hectare applies 700kg compound fertilizer, and early or mid July carefully pulls down soil layer at rhizome with small spade, using cutting edge of a knife or a sword
Sharp scalpel scratches the epidermis at Chinese herbaceous peony rhizome, surrounds the slow trickle irrigation of rhizome portion with the basic element of cell division of 500mg/L, dosage is
1L, then dip in 900 times of carbendazim disinfection wounds with cotton balls, then earthing, the trickle irrigation basic element of cell division weekly later, trickle irrigation 3 times altogether, 8
Middle of the month per hectare applies 3000kg cake fertilizer.
Suo Shi compound fertilizer N, P, K composition ratio: 10:35:15；Applied cake fertilizer be with soybean extract oil residue and water in mass ratio
1:3 mixed fermentation is decomposed to be obtained, and when use is diluted with water 40 times.
A kind of method for promoting Chinese herbaceous peony bud to sprout of embodiment 2
The method are as follows:
Mid or late June per hectare applies 600kg compound fertilizer, and early or mid July carefully pulls down soil layer at rhizome with small spade, using cutting edge of a knife or a sword
Sharp scalpel scratches the epidermis at Chinese herbaceous peony rhizome, surrounds the slow trickle irrigation of rhizome portion with the basic element of cell division of 350mg/L, dosage is
1L, then dip in 800 times of carbendazim disinfection wounds with cotton balls, then earthing, the trickle irrigation basic element of cell division weekly later, trickle irrigation 5 times altogether, 8
Middle of the month per hectare applies 2250kg cake fertilizer.
Suo Shi compound fertilizer N, P, K composition ratio: 10:20:15；Applied cake fertilizer be with soybean extract oil residue and water in mass ratio
1:5 mixed fermentation is decomposed to be obtained, and when use is diluted with water 40 times.
Influence of the test example distinct methods to promoting Chinese herbaceous peony bud to sprout
To probe into the influence that distinct methods sprout Chinese herbaceous peony bud, the present invention, which removes, uses above-described embodiment 1,2 methods, also following
Method is stated as control:
Contrast method 1:6 the middle ten days and the last ten days month per hectare applies 700kg compound fertilizer, and early or mid July is carefully pulled down at rhizome with small spade
Soil layer scratches the epidermis at Chinese herbaceous peony rhizome using sharp scalpel, is slowly dripped with the basic element of cell division of 500mg/L around rhizome portion
It fills, dosage 1L, then dips in 900 times of carbendazim disinfection wounds with cotton balls, then earthing, later the trickle irrigation basic element of cell division weekly, altogether
Trickle irrigation 3 times, mid-August, per hectare applied 3000kg cake fertilizer.Suo Shi compound fertilizer N, P, K composition ratio: 20:15:15；Applied cake fertilizer is
With soybean extract oil residue and water, 1:3 mixed fermentation is decomposed in mass ratio obtains, and when use is diluted with water 40 times.
Contrast method 2:6 the middle ten days and the last ten days month per hectare applies 700kg compound fertilizer, and early or mid August is carefully pulled down at rhizome with small spade
Soil layer scratches the epidermis at Chinese herbaceous peony rhizome using sharp scalpel, is slowly dripped with the basic element of cell division of 500mg/L around rhizome portion
It fills, dosage 1L, then dips in 900 times of carbendazim disinfection wounds with cotton balls, then earthing, later the trickle irrigation basic element of cell division weekly, altogether
Trickle irrigation 3 times, mid-September, per hectare applied 3000kg cake fertilizer.Suo Shi compound fertilizer N, P, K composition ratio: 10:35:15；Applied cake fertilizer is
With soybean extract oil residue and water, 1:3 mixed fermentation is decomposed in mass ratio obtains, and when use is diluted with water 40 times.
The first tenday period of a month carefully pull down soil layer at rhizome with small spade in the contrast method 3:7 month, scratch Peony Root using sharp scalpel
Epidermis at stem surrounds the slow trickle irrigation of rhizome portion, dosage 1L with the basic element of cell division of 500mg/L, then is dipped in more than 900 times with cotton balls
Bacterium spirit sterilizes wound, then earthing, later the trickle irrigation basic element of cell division weekly, altogether trickle irrigation 3 times.
Contrast method 4:6 the middle ten days and the last ten days month per hectare applies 700kg compound fertilizer, and mid-August, per hectare applied 3000kg cake fertilizer；It is applied
Compound fertilizer's N, P, K composition ratio: 10:35:15；Applied cake fertilizer is with soybean extract oil residue and water 1:3 mixed fermentation in mass ratio
Decomposed to obtain, when use, is diluted with water 40 times.
210 plants of Peony Roots are chosen, are divided into seven groups, every group 30 plants, every group is respectively adopted corresponding method and is tested:
Group 1: promote the sprouting of Chinese herbaceous peony bud using 1 method of embodiment；
Group 2: promote the sprouting of Chinese herbaceous peony bud using 2 method of embodiment；
Group 3: promote the sprouting of Chinese herbaceous peony bud using contrast method 1；
Group 4: promote the sprouting of Chinese herbaceous peony bud using contrast method 2；
Group 5: promote the sprouting of Chinese herbaceous peony bud using contrast method 3；
Group 6: promote the sprouting of Chinese herbaceous peony bud using contrast method 4；
Group 7: any processing method is not used, as blank control group.
According to above method to four Nian Sheng ' odd liquid distilled from honeysuckle flowers or lotus leaves frost ', ' poplar prince wife goes out to bathe ' Peony Root handle, by the end of October statistically
The ratio (bud stem ratio) of lower bud and annual shoot number.Shown in table 1 specific as follows.
The influence that 1 distinct methods of table sprout Chinese herbaceous peony bud
By upper table 1 it is found that being handled using 1 method of embodiment, odd liquid distilled from honeysuckle flowers or lotus leaves frost ' the Chinese herbaceous peony bud stem of four Nian Sheng ', which frequently compares, to be improved
67.7%, than being applied alone the processing of the 500mg/L basic element of cell division to improve 40.1%；Four Nian Sheng ' poplar prince wifes go out to bathe ' control frequently of Chinese herbaceous peony bud stem
88.2% is improved, than being applied alone basic element of cell division processing to improve 38.9%.In summary, the method for the present invention is with being remarkably improved Chinese herbaceous peony
The proliferation and growth of lower bud.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of method for promoting Chinese herbaceous peony bud to sprout, which is characterized in that the described method includes: mid or late June per hectare apply 600~
750kg compound fertilizer carries out pouring root with the basic element of cell division, later the trickle irrigation basic element of cell division weekly, altogether trickle irrigation 2~5 times, mid-August
Apply cake fertilizer.
2. the method according to claim 1, wherein Suo Shi compound fertilizer N, P, K composition ratio: 10:20~40:15
~20.
3. the method according to claim 1, wherein carrying out pouring root with the basic element of cell division in early or mid July.
4. method according to claim 1, which is characterized in that the basic element of cell division is total to trickle irrigation 3 times.
5. the method according to claim 1, wherein with the method for basic element of cell division pouring root are as follows: pull down Peony Root
Soil layer at stem scratches the epidermis at Chinese herbaceous peony rhizome with knife, is slowly dripped with the basic element of cell division of 350~500mg/L around rhizome portion
It fills, then sterilizes wound with fungicide, then earthing.
6. according to the method described in claim 4, it is characterized in that, the dosage of the basic element of cell division is 800~1200mL.
7. according to the method described in claim 4, it is characterized in that, the fungicide is carbendazim.
8. the method according to claim 4 or 6, which is characterized in that the concentration of carbendazim is 800~1000 times of liquid.
9. the method according to claim 1, wherein applied cake fertilizer be with soybean extract oil residue and water in mass ratio
The mixed fermentation of 1:3~5 is decomposed to be obtained, and when use is diluted with water 30~40 times.
10. according to claim 1 or method described in 9, which is characterized in that per hectare applies 2250~3000kg cake fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811504625.6A CN109496493B (en) | 2018-12-10 | 2018-12-10 | Method for promoting germination of peony buds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811504625.6A CN109496493B (en) | 2018-12-10 | 2018-12-10 | Method for promoting germination of peony buds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109496493A true CN109496493A (en) | 2019-03-22 |
| CN109496493B CN109496493B (en) | 2021-05-07 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521543A (en) * | 2015-01-26 | 2015-04-22 | 重庆文理学院 | Oil peony seedling cultivation method |
| CN105009902A (en) * | 2015-07-28 | 2015-11-04 | 渠县金穗农业科技有限公司 | Mixed interplant method for white peony root division breeding |
| CN107873517A (en) * | 2017-11-14 | 2018-04-06 | 沈阳农业大学 | A kind of method by Chinese herbaceous peony cotyledon evoked callus regenerated adventitious bud |
-
2018
- 2018-12-10 CN CN201811504625.6A patent/CN109496493B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521543A (en) * | 2015-01-26 | 2015-04-22 | 重庆文理学院 | Oil peony seedling cultivation method |
| CN105009902A (en) * | 2015-07-28 | 2015-11-04 | 渠县金穗农业科技有限公司 | Mixed interplant method for white peony root division breeding |
| CN107873517A (en) * | 2017-11-14 | 2018-04-06 | 沈阳农业大学 | A kind of method by Chinese herbaceous peony cotyledon evoked callus regenerated adventitious bud |
Non-Patent Citations (3)
| Title |
|---|
| 吴红娟 等: ""芍药不同品种启动培养比较及‘朱砂判’丛生芽诱导的研究"", 《内蒙古农业大学学报(自然科学版)》 * |
| 许世磊: ""芍药植株促芽的研究"", 《中国优秀硕士学位论文全文数据库-农业科技辑》 * |
| 赵竹梅: ""大通地区芍药栽培技术"", 《青海农林科技》 * |
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