CN109467140A - A kind of new method of urban river deodorization - Google Patents

A kind of new method of urban river deodorization Download PDF

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Publication number
CN109467140A
CN109467140A CN201811195699.6A CN201811195699A CN109467140A CN 109467140 A CN109467140 A CN 109467140A CN 201811195699 A CN201811195699 A CN 201811195699A CN 109467140 A CN109467140 A CN 109467140A
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China
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ammonia
water
smelly
urban river
bacterial strain
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朱晔
侯丽华
刘丹
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Tianjin University of Science and Technology
Hebei University of Engineering
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Tianjin University of Science and Technology
Hebei University of Engineering
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Priority to CN201811195699.6A priority Critical patent/CN109467140A/en
Publication of CN109467140A publication Critical patent/CN109467140A/en
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/007Contaminated open waterways, rivers, lakes or ponds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00Specific treatment goals
    • C02F2303/02Odour removal or prevention of malodour

Abstract

The present invention relates to a kind of new methods of urban river deodorization, and described method includes following steps: (1) taking out the smelly water body in urban river, screen isolated strains from industrial wastewater and the smelly water body of sanitary sewage respectively;(2) verify whether separated bacterial strain has stink generation;(3) inhibit to produce smelly strain growth by adjusting the means that different pH, addition different preservatives and the two combine, reduce ammonia-nitrogen content, control stink generates.The method of the present invention inhibits to produce smelly strain growth by adjusting the comprehensive means that pH value, addition preservative and the two combine, and ammonia-nitrogen content is reduced, to eliminate the generation of stink.Operation of the present invention process is simple, highly-safe, will not impact to operator, and a large amount of uses will not impact environment, not will cause secondary pollution, and more environmentally-friendly, easy to use and production cost is lower, has preferable economic value and the ecological value.

Description

A kind of new method of urban river deodorization
Technical field
The invention belongs to microorganisms technical field and water body deodorization technology field, especially a kind of urban river deodorization it is new Method.
Background technique
In order to remove urban river stink, the improvement to smelly water body mainly includes physical method, chemical method, bioanalysis and river Road aeration etc..Physical method mainly includes cutting the hydraulic engineerings such as dirt, water transfer, dredging;Chemical method such as chemical reagent removes algae, adds Enter the precipitating that molysite promotes phosphorus;Biological method mainly has artificial wetland treatment, hydrophyte restoration, biological prosthetic etc..
Process above has cost of investment height, treatment effect slow effect, the treatment effect disadvantage larger by seasonal effect.
By retrieval, patent publication us relevant to present patent application is not yet found.
Summary of the invention
Place that the purpose of the present invention is to overcome the deficiency in the prior art, provides a kind of new method of urban river deodorization, should Method operating process is simple, highly-safe, will not impact to operator, and a large amount of uses will not cause shadow to environment It rings, not will cause secondary pollution, more environmentally-friendly, easy to use and production cost is lower, has preferable economic value and life State value.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of new method of urban river deodorization, described method includes following steps:
(1) river smelly water body in urban is taken out, wherein urban river includes the industrial wastewater and sanitary sewage being discharged into, The smelly water body of part industrial wastewater and the smelly water body of sanitary sewage are taken out respectively, respectively from industrial wastewater and the smelly water of sanitary sewage Isolated strains are screened in body;
(2) verify whether separated bacterial strain has stink generation, water body is smelly to decompose the sulphur overflowed including sulfur-bearing, nitrogen organic Change caused by hydrogen, ammonia;And ammonia nitrogen refer in water in the form of free ammonia and ammonium ion existing for nitrogen, as long as therefore reducing ammonia in water body Nitrogen content reduces free ammonia, ammonia concentration, just can control stink generation;
(3) inhibit to produce smelly strain growth by adjusting the means that different pH, addition different preservatives and the two combine, reduce Ammonia-nitrogen content, control stink generate.
And, the specific steps are as follows:
(1) screen isolated strains: screening and separation to bacterial strain are realized by cultivating on solid plate;
Screening and separation that YPD and LB solid medium carries out bacterial strain are chosen, the smelly water body of industrial wastewater and life is dirty The smelly water body of water dilutes 10 respectively-1With 10-2Two gradients take 200ul sample liquid to distinguish even spread and arrive not to be diluted to compare On two kinds of culture mediums, 48h is cultivated at 30 DEG C and 37 DEG C respectively later;
The secondary culture on corresponding culture medium respectively by obtained different strains, after obtaining single colonie, in respective liquid After being cultivated in culture medium, with glycerol cold storage in -80 DEG C of refrigerators, for use;
(2) produce hydrogen sulfide verifying: by isolated strain inoculated into corresponding fluid nutrient medium, being trained by corresponding conditions It supports 24~48 h and prepares bacteria suspension;Under aseptic condition, it is inoculated in produces H respectively2S is verified on slant medium, to ensure result Accuracy uses sterile water as compareing, is inoculated in sterile water under aseptic condition and produces H2S is verified on slant medium, constant temperature training It supports, whether observation bacterial strain generates hydrogen sulfide gas;
(3) ammonia verifying is produced: by isolated strain inoculated into corresponding fluid nutrient medium, by corresponding conditions culture 24~48h prepares bacteria suspension, under aseptic condition, takes 1 × 10 respectively6A/ml bacterium is transferred in respective liquid culture medium, wherein Fluid nutrient medium is placed in 50ml triangular flask, is put into wet litmus red test paper, corresponding revolving speed in the envelope bottle film of triangular flask 24~48h is cultivated, as a control group with sterile water, whether observation litmus red test paper becomes blue, if becoming blue, proves separation institute It obtains bacterial strain and generates ammonia;
(4) strain growth is inhibited, and control stink generates:
Ammonia-nitrogen content in water body is reduced, free ammonia, ammonia concentration is reduced: under aseptic condition, isolated bacterial strain being existed 24~48h of culture is carried out in corresponding fluid nutrient medium, bacteria suspension is prepared, takes 1 × 106A/ml bacterium transfer respectively it is different into pH, In the fluid nutrient medium for adding different preservatives, 150r/min, 37 DEG C of constant temperature incubations to stationary phase measure its absorbance value later And ammonia-nitrogen content, litmus red test paper discoloration is observed, is inhibited and produces smelly strain growth, reduces ammonia-nitrogen content, is controlled smelly The means that different pH, addition different preservatives and the two that taste generates combine can carry out deodorization to urban river.
Moreover, (4) middle difference pH is 3,4,5,6,7,8 to the step;The different preservatives and its whole quality of addition are dense Degree is respectively 0.25% sodium benzoate, 0.05% dehydroactic acid sodium, 3% sodium lactate.
Moreover, the step means that (4) middle difference pH, addition different preservatives and the two combine are as follows:
When adjusting the pH≤4 of smelly water body, one or more of 3 kinds of preservatives are added;When the smelly water of adjusting When the pH=5 of body, the sodium benzoate that whole mass concentration is 0.25% is added;
Alternatively, adding one or more of 3 kinds of preservatives when adjusting the pH≤3 of smelly water body;It is sent out when adjusting When the pH of smelly water body≤4, the sodium benzoate that whole mass concentration is 0.25% and/or the dehydrogenation that whole mass concentration is 0.05% are added Sodium acetate;When adjusting the pH≤5 of smelly water body, the sodium benzoate that whole mass concentration is 0.25% is added.
Moreover, the step (2) in H2S verify slant medium the preparation method is as follows: being added in every 100ml phenol red 0.0025g, ferrous sulfate 0.02g, tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar 1.2g.
Moreover, the step (4) in the different fluid nutrient medium of pH the preparation method is as follows: adjusting fluid nutrient medium respectively PH is 3,4,5,6,7,8, and is placed it in high-pressure sterilizing pot in 115 DEG C of sterilizing 20min.
Moreover, it is described addition different preservatives fluid nutrient medium the preparation method is as follows:
1. 0.25% sodium benzoate Liquid Culture based sols: under sterile conditions, sodium benzoate 0.25g is first weighed, and It is dissolved in the sterilized fluid nutrient medium of 100ml, shakes up, be filtered later with sterilised membrane filter device and degerming, be placed in 4 DEG C Refrigerator saves backup;
2. 0.05% dehydroactic acid sodium Liquid Culture based sols: aseptically, dehydroactic acid sodium 0.05g is first weighed, It is dissolved in the sterilized fluid nutrient medium of 100ml, shakes up again, be filtered simultaneously degerming with sterilised membrane filter device later, be placed in 4 DEG C refrigerator saves backup;
3. 3% sodium lactate Liquid Culture based sols: under aseptic condition, drawing 3ml mass concentration with liquid-transfering gun is 60% Sodium lactate solution, and be added into the sterilized fluid nutrient medium of 100ml, it shakes up, is filtered later with sterilised membrane filter device And degerming, it is placed in 4 DEG C of refrigerator and saves backup.
Moreover, the step (4) in take 1 × 106A/ml bacterium is transferred respectively into the liquid of pH difference, addition different preservatives PH in culture medium is different, addition different preservatives fluid nutrient mediums are as follows: 6 kinds of different pH value anti-corrosions different from 3 kinds respectively Agent combination, the fluid nutrient medium being prepared.
Moreover, the step (1) in isolated 5 plants of bacterium are screened from the smelly water body of industrial wastewater, from sanitary sewage from Isolated 4 plants of bacterium in water body;The step (2) in separating obtained bacterial strain without hydrogen sulfide gas generate;The step (3) in point Litmus red test paper from obtained strains becomes blue, then proves there is ammonia generation.
Moreover, the step (2) in separating obtained bacterial strain without hydrogen sulfide gas generate, illustrate the taken city of the method Without the bacterial strain for producing hydrogen sulfide gas in river water sample;The step (3) in separating obtained bacterial strain have an ammonia generation, described in explanation There is the bacterial strain for producing ammonia in the taken urban river water sample of method, illustrates in the smelly water body of urban river containing the bacterium for generating ammonia Strain.
The advantages of present invention obtains and good effect are as follows:
The present invention fundamentally solves urban river malodor problem, solves existing by producing smelly bacterial strain in screening urban river Harm of some smelly water bodys of urban river to city dweller and urban environment, this method is by adjusting pH value, addition preservative And the comprehensive means that the two combines inhibit to produce smelly strain growth, ammonia-nitrogen content are reduced, to eliminate the generation of stink.Present invention behaviour It is simple to make process, it is highly-safe, operator will not be impacted, a large amount of uses will not impact environment, will not Secondary pollution is caused, more environmentally-friendly, easy to use and production cost is lower, has preferable economic value and the ecological value.
Specific embodiment
The embodiment of the present invention is described in detail below, it should be noted that the present embodiment is narrative, is not limited , this does not limit the scope of protection of the present invention.
Raw material used in the present invention is unless otherwise specified conventional commercial product;Used in the present invention Method is unless otherwise specified the conventional method of this field.
Embodiment 1
A kind of new method of urban river deodorization, described method includes following steps:
(1) river smelly water body in urban is taken out, wherein urban river includes the industrial wastewater and sanitary sewage being discharged into, The smelly water body of part industrial wastewater and the smelly water body of sanitary sewage are taken out respectively, respectively from industrial wastewater and the smelly water of sanitary sewage Isolated strains are screened in body;
(2) verify whether separated bacterial strain has stink generation, water body is smelly to decompose the sulphur overflowed including sulfur-bearing, nitrogen organic Change caused by hydrogen, ammonia;And ammonia nitrogen refers in water with free ammonia (NH3) and ammonium ion (NH4+) nitrogen existing for form, as long as therefore dropping Ammonia-nitrogen content in low water body reduces free ammonia, ammonia concentration, just can control stink generation;
(3) inhibit to produce smelly strain growth by adjusting the means that different pH, addition different preservatives and the two combine, reduce Ammonia-nitrogen content, control stink generate.
Specifically, steps are as follows for step:
(1) screen isolated strains: screening and separation to bacterial strain are mainly realized by cultivating on solid plate.It chooses YPD and LB solid medium carries out the screening and separation of bacterial strain, and industrial wastewater and sanitary sewage are diluted 10 respectively-1With 10-2 Two gradients are taken on 200ul sample liquid difference even spread to two kinds of culture mediums, with not being diluted to compare later respectively at 30 DEG C 48h is cultivated at 37 DEG C.The secondary culture on corresponding culture medium respectively by obtained different strains, after obtaining single colonie, After being cultivated in respective liquid culture medium, with glycerol cold storage in -80 DEG C of refrigerators, for use.The results show that the screening point from industrial wastewater From obtaining 5 plants of bacterium, isolated 4 plants of bacterium from sanitary sewage.
(2) produce hydrogen sulfide verifying: by isolated strain inoculated into corresponding fluid nutrient medium, being trained by corresponding conditions It supports 24~48 h and prepares bacteria suspension;Under aseptic condition, it is inoculated in produces H respectively2S is verified on slant medium, to ensure result Accuracy uses sterile water as compareing, is inoculated in sterile water under aseptic condition and produces H2S is verified on slant medium, constant temperature training It supports.Separating obtained bacterial strain is generated without hydrogen sulfide gas.
(3) ammonia verifying is produced: by isolated strain inoculated into corresponding fluid nutrient medium, by corresponding conditions culture 24~48h prepares bacteria suspension, under aseptic condition, takes 1 × 10 respectively6A/ml bacterium is transferred in respective liquid culture medium, wherein Fluid nutrient medium is placed in 50ml triangular flask, is put into wet litmus red test paper, corresponding revolving speed in the envelope bottle film of triangular flask 24~48h is cultivated, as a control group with sterile water, observation experiment result is that separating obtained bacterial strain litmus red test paper becomes blue, There is ammonia generation.
(4) inhibit strain growth, reduce ammonia-nitrogen content, control stink generates: under aseptic condition, by isolated bacterial strain 24~48h is cultivated in LB liquid medium respectively, prepares bacteria suspension, takes 1 × 10 respectively when each strain growth is to logarithmic phase6 In the LB liquid medium that it is 3,4,5,6,7,8 into pH that a/ml thallus, which is transferred, setting speed 150r/min, 37 DEG C of constant temperature trainings It supports to stationary phase, measures its absorbance value and ammonia-nitrogen content later, observe litmus red test paper discoloration, being inhibited, it is smelly to produce Strain growth reduces ammonia-nitrogen content, the means that different pH, addition different preservatives and the two that control stink generates combine, i.e., Deodorization can be carried out to urban river.
Specific Parameter Conditions and experimental result are shown in Table 1, table 2 and table 3.
Embodiment 2
A kind of new method of urban river deodorization, described method includes following steps:
(1) river smelly water body in urban is taken out, wherein urban river includes the industrial wastewater and sanitary sewage being discharged into, The smelly water body of part industrial wastewater and the smelly water body of sanitary sewage are taken out respectively, respectively from industrial wastewater and the smelly water of sanitary sewage Isolated strains are screened in body;
(2) verify whether separated bacterial strain has stink generation, water body is smelly to decompose the sulphur overflowed including sulfur-bearing, nitrogen organic Change caused by hydrogen, ammonia;And ammonia nitrogen refers in water with free ammonia (NH3) and ammonium ion (NH4+) nitrogen existing for form, as long as therefore dropping Ammonia-nitrogen content in low water body reduces free ammonia, ammonia concentration, just can control stink generation;
(3) inhibit to produce smelly strain growth by adjusting the means that different pH, addition different preservatives and the two combine, reduce Ammonia-nitrogen content, control stink generate.
Specifically, steps are as follows for step:
(1) screen isolated strains: screening and separation to bacterial strain are mainly realized by cultivating on solid plate.It chooses YPD and LB solid medium carries out the screening and separation of bacterial strain, and industrial wastewater and sanitary sewage are diluted 10 respectively-1With 10-2 Two gradients are taken on 200ul sample liquid difference even spread to two kinds of culture mediums, with not being diluted to compare later respectively at 30 DEG C 48h is cultivated at 37 DEG C.The secondary culture on corresponding culture medium respectively by obtained different strains, after obtaining single colonie, After being cultivated in respective liquid culture medium, with glycerol cold storage in -80 DEG C of refrigerators, for use.The results show that the screening point from industrial wastewater From obtaining 5 plants of bacterium, isolated 4 plants of bacterium from sanitary sewage.
(2) produce hydrogen sulfide verifying: by isolated strain inoculated into corresponding fluid nutrient medium, being trained by corresponding conditions It supports 24~48 h and prepares bacteria suspension;Under aseptic condition, it is inoculated in produces H respectively2S is verified on slant medium, to ensure result Accuracy uses sterile water as compareing, is inoculated in sterile water under aseptic condition and produces H2S is verified on slant medium, constant temperature training It supports.Separating obtained bacterial strain is generated without hydrogen sulfide gas.
(3) ammonia verifying is produced: by isolated strain inoculated into corresponding fluid nutrient medium, by corresponding conditions culture 24~48h prepares bacteria suspension, under aseptic condition, takes 1 × 10 respectively6A/ml bacterium is transferred in respective liquid culture medium, wherein Fluid nutrient medium is placed in 50ml triangular flask, is put into wet litmus red test paper, corresponding revolving speed in the envelope bottle film of triangular flask 24~48h is cultivated, as a control group with sterile water, observation experiment result is that separating obtained bacterial strain litmus red test paper becomes blue, There is ammonia generation.
(4) inhibit strain growth, reduce ammonia-nitrogen content, control stink generates: under aseptic condition, by isolated bacterial strain 24~48h is cultivated in LB liquid medium respectively, prepares bacteria suspension, takes 1 × 10 respectively when each strain growth is to logarithmic phase6 A/ml thallus transfer into prepared be not added with preservative, addition 0.25% sodium benzoate, 0.05% dehydroactic acid sodium and In the LB liquid medium of 3% sodium lactate.Revolving speed is 150r/min, and 37 DEG C of constant temperature incubations to stationary phase measure its extinction later Angle value and ammonia-nitrogen content observe litmus red test paper discoloration, are inhibited and produce smelly strain growth, reduce ammonia-nitrogen content, control The means that different pH, addition different preservatives and the two that stink processed generates combine can carry out deodorization to urban river.
Specific Parameter Conditions and experimental result are shown in Table 1, table 2 and table 3.
Embodiment 3
A kind of new method of urban river deodorization, described method includes following steps:
(1) river smelly water body in urban is taken out, wherein urban river includes the industrial wastewater and sanitary sewage being discharged into, The smelly water body of part industrial wastewater and the smelly water body of sanitary sewage are taken out respectively, respectively from industrial wastewater and the smelly water of sanitary sewage Isolated strains are screened in body;
(2) verify whether separated bacterial strain has stink generation, water body is smelly to decompose the sulphur overflowed including sulfur-bearing, nitrogen organic Change caused by hydrogen, ammonia;And ammonia nitrogen refers in water with free ammonia (NH3) and ammonium ion (NH4+) nitrogen existing for form, as long as therefore dropping Ammonia-nitrogen content in low water body reduces free ammonia, ammonia concentration, just can control stink generation;
(3) inhibit to produce smelly strain growth by adjusting the means that different pH, addition different preservatives and the two combine, reduce Ammonia-nitrogen content, control stink generate.
Specifically, steps are as follows for step:
(1) screen isolated strains: screening and separation to bacterial strain are mainly realized by cultivating on solid plate.It chooses YPD and LB solid medium carries out the screening and separation of bacterial strain, and industrial wastewater and sanitary sewage are diluted 10 respectively-1With 10-2 Two gradients are taken on 200ul sample liquid difference even spread to two kinds of culture mediums, with not being diluted to compare later respectively at 30 DEG C 48h is cultivated at 37 DEG C.The secondary culture on corresponding culture medium respectively by obtained different strains, after obtaining single colonie, After being cultivated in respective liquid culture medium, with glycerol cold storage in -80 DEG C of refrigerators, for use.The results show that the screening point from industrial wastewater From obtaining 5 plants of bacterium, isolated 4 plants of bacterium from sanitary sewage.
(2) produce hydrogen sulfide verifying: by isolated strain inoculated into corresponding fluid nutrient medium, being trained by corresponding conditions It supports 24~48 h and prepares bacteria suspension;Under aseptic condition, it is inoculated in produces H respectively2S is verified on slant medium, to ensure result Accuracy uses sterile water as compareing, is inoculated in sterile water under aseptic condition and produces H2S is verified on slant medium, constant temperature training It supports.Separating obtained bacterial strain is generated without hydrogen sulfide gas.
(3) ammonia verifying is produced: by isolated strain inoculated into corresponding fluid nutrient medium, by corresponding conditions culture 24~48h prepares bacteria suspension, under aseptic condition, 1 × 106/ml bacterium is taken to transfer in respective liquid culture medium respectively, wherein Fluid nutrient medium is placed in 50ml triangular flask, is put into wet litmus red test paper, corresponding revolving speed in the envelope bottle film of triangular flask 24~48h is cultivated, as a control group with sterile water, observation experiment result is that separating obtained bacterial strain litmus red test paper becomes blue, There is ammonia generation.
(4) inhibit strain growth, reduce ammonia-nitrogen content, control stink generates: under aseptic condition, by isolated bacterial strain 24~48h is cultivated in LB liquid medium respectively, prepares bacteria suspension, takes 1 × 10 respectively when each strain growth is to logarithmic phase6 A/ml thallus, which is transferred, to be 3 into the pH prepared and adds 0.25% sodium benzoate, 0.05% dehydroactic acid sodium and 3% respectively Sodium lactate;PH is 4 and adds 0.25% sodium benzoate, 0.05% dehydroactic acid sodium and 3% sodium lactate respectively;PH is 5 and difference Add 0.25% sodium benzoate, 0.05% dehydroactic acid sodium and 3% sodium lactate;PH is 6 and adds 0.25% sodium benzoate respectively, 0.05% dehydroactic acid sodium and 3% sodium lactate;PH is 7 and adds 0.25% sodium benzoate respectively, 0.05% dehydroactic acid sodium and 3% sodium lactate;PH is 8 and adds 0.25% sodium benzoate, the LB liquid of 0.05% dehydroactic acid sodium and 3% sodium lactate respectively In culture medium.Revolving speed is 150r/min, and 37 DEG C of constant temperature incubations to stationary phase measure its absorbance value and ammonia-nitrogen content later, is seen Litmus red test paper discoloration is examined, is inhibited and produces smelly strain growth, reduces ammonia-nitrogen content, the difference that control stink generates The means that pH, addition different preservatives and the two combine can carry out deodorization to urban river.
Specific Parameter Conditions and experimental result are shown in Table 1, table 2 and table 3.
Each embodiment absorbance value of the method for the present invention is measured, ammonia-nitrogen content observes litmus red test paper discoloration result It is as follows:
Each embodiment absorbance value table of 1 the method for the present invention of table
From table 1 it follows that urban river strain growth can be inhibited by adding 3 kinds of preservatives when pH≤4, when pH=5, Urban river strain growth can be inhibited by adding 0.25% sodium benzoate.
Each embodiment ammonia-nitrogen content table (mg/L) of 2 the method for the present invention of table
From Table 2, it can be seen that urban river ammonia-nitrogen content, pH=can be effectively reduced by adding 3 kinds of preservatives when pH≤4 When 5, urban river ammonia-nitrogen content can be effectively reduced in 0.25% sodium benzoate of addition.
Each embodiment red reindeer moss discoloration table of 3 the method for the present invention of table
Note: "+" indicates that litmus red test paper becomes blue, and "-" indicates result litmus red test paper without discoloration.
From Table 2, it can be seen that it is non-discolouring to add 3 kinds of preservative litmus red test papers when pH≤3, and when pH≤4, addition 0.25% sodium benzoate and the litmus red test paper of 0.05% dehydroactic acid sodium are non-discolouring, and when pH≤5 adds 0.25% benzoic acid The litmus red test paper of sodium is non-discolouring.No ammonia overflows, and can effectively eliminate urban river stink.
Embodiment 4
A kind of new method of urban river deodorization, described method includes following steps:
(1) river smelly water body in urban is taken out, wherein urban river includes the industrial wastewater and sanitary sewage being discharged into, The smelly water body of part industrial wastewater and the smelly water body of sanitary sewage are taken out respectively, respectively from industrial wastewater and the smelly water of sanitary sewage Isolated strains are screened in body;
(2) verify whether separated bacterial strain has stink generation, water body is smelly to decompose the sulphur overflowed including sulfur-bearing, nitrogen organic Change caused by hydrogen, ammonia;And ammonia nitrogen refer in water in the form of free ammonia and ammonium ion existing for nitrogen, as long as therefore reducing ammonia in water body Nitrogen content reduces free ammonia, ammonia concentration, just can control stink generation;
(3) inhibit to produce smelly strain growth by adjusting the means that different pH, addition different preservatives and the two combine, reduce Ammonia-nitrogen content, control stink generate.
More preferably, the specific steps are as follows:
(1) screen isolated strains: screening and separation to bacterial strain are realized by cultivating on solid plate;
Screening and separation that YPD and LB solid medium carries out bacterial strain are chosen, the smelly water body of industrial wastewater and life is dirty The smelly water body of water dilutes 10 respectively-1With 10-2Two gradients take 200ul sample liquid to distinguish even spread and arrive not to be diluted to compare On two kinds of culture mediums, 48h is cultivated at 30 DEG C and 37 DEG C respectively later;
The secondary culture on corresponding culture medium respectively by obtained different strains, after obtaining single colonie, in respective liquid After being cultivated in culture medium, with glycerol cold storage in -80 DEG C of refrigerators, for use;
(2) produce hydrogen sulfide verifying: by isolated strain inoculated into corresponding fluid nutrient medium, being trained by corresponding conditions It supports 24~48 h and prepares bacteria suspension;Under aseptic condition, it is inoculated in produces H respectively2S is verified on slant medium, to ensure result Accuracy uses sterile water as compareing, is inoculated in sterile water under aseptic condition and produces H2S is verified on slant medium, constant temperature training It supports, whether observation bacterial strain generates hydrogen sulfide gas;
(3) ammonia verifying is produced: by isolated strain inoculated into corresponding fluid nutrient medium, by corresponding conditions culture 24~48h prepares bacteria suspension, under aseptic condition, takes 1 × 10 respectively6A/ml bacterium is transferred in respective liquid culture medium, wherein Fluid nutrient medium is placed in 50ml triangular flask, is put into wet litmus red test paper, corresponding revolving speed in the envelope bottle film of triangular flask 24~48h is cultivated, as a control group with sterile water, whether observation litmus red test paper becomes blue, if becoming blue, proves separation institute It obtains bacterial strain and generates ammonia;
(4) strain growth is inhibited, and control stink generates:
Ammonia-nitrogen content in water body is reduced, free ammonia, ammonia concentration is reduced: under aseptic condition, isolated bacterial strain being existed 24~48h of culture is carried out in corresponding fluid nutrient medium, bacteria suspension is prepared, takes 1 × 106A/ml bacterium transfer respectively it is different into pH, In the fluid nutrient medium for adding different preservatives, 150r/min, 37 DEG C of constant temperature incubations to stationary phase measure its absorbance value later And ammonia-nitrogen content, litmus red test paper discoloration is observed, is inhibited and produces smelly strain growth, reduces ammonia-nitrogen content, is controlled smelly The means that different pH, addition different preservatives and the two that taste generates combine can carry out deodorization to urban river.
More preferably, (4) middle difference pH is 3,4,5,6,7,8 to the step;The different preservatives and its whole quality of addition Concentration is respectively 0.25% sodium benzoate, 0.05% dehydroactic acid sodium, 3% sodium lactate.
More preferably, the step means that (4) middle difference pH, addition different preservatives and the two combine are as follows:
When adjusting the pH≤4 of smelly water body, one or more of 3 kinds of preservatives are added;When the smelly water of adjusting When the pH=5 of body, the sodium benzoate that whole mass concentration is 0.25% is added;
More preferably, when adjusting the pH≤3 of smelly water body, one or more of 3 kinds of preservatives are added;Work as adjusting When the pH of smelly water body≤4, add sodium benzoate that whole mass concentration is 0.25% and/or whole mass concentration be 0.05% it is de- Hydroacetic acid sodium;When adjusting the pH≤5 of smelly water body, the sodium benzoate that whole mass concentration is 0.25% is added.
More preferably, the step (2) in H2S verify slant medium the preparation method is as follows: being added in every 100ml phenol red 0.0025g, ferrous sulfate 0.02g, tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar 1.2g.
More preferably, the step (4) in the different fluid nutrient medium of pH the preparation method is as follows: can be used it is highly-safe, It is 3,4,5,6,7,8 that the acid or alkali of low cost adjust fluid nutrient medium pH respectively, and is placed it in high-pressure sterilizing pot in 115 DEG C Sterilize 20min.
More preferably, it is described addition different preservatives fluid nutrient medium the preparation method is as follows:
1. 0.25% sodium benzoate Liquid Culture based sols: under sterile conditions, sodium benzoate 0.25g is first weighed, and It is dissolved in the sterilized fluid nutrient medium of 100ml, shakes up, be filtered later with sterilised membrane filter device and degerming, be placed in 4 DEG C Refrigerator saves backup;
2. 0.05% dehydroactic acid sodium Liquid Culture based sols: aseptically, dehydroactic acid sodium 0.05g is first weighed, It is dissolved in the sterilized fluid nutrient medium of 100ml, shakes up again, be filtered simultaneously degerming with sterilised membrane filter device later, be placed in 4 DEG C refrigerator saves backup;
3. 3% sodium lactate Liquid Culture based sols: under aseptic condition, drawing 3ml mass concentration with liquid-transfering gun is 60% Sodium lactate solution, and be added into the sterilized fluid nutrient medium of 100ml, it shakes up, is filtered later with sterilised membrane filter device And degerming, it is placed in 4 DEG C of refrigerator and saves backup.
More preferably, the step (4) in take 1 × 106A/ml bacterium is transferred respectively into the liquid of pH difference, addition different preservatives PH in body culture medium is different, addition different preservatives fluid nutrient mediums are as follows: 6 kinds of different pH value respectively with 3 kinds of different preventings Rotten agent combination, the fluid nutrient medium being prepared.
More preferably, the step (1) in isolated 5 plants of bacterium are screened from the smelly water body of industrial wastewater, from sanitary sewage send out Isolated 4 plants of bacterium from water body;The step (2) in separating obtained bacterial strain without hydrogen sulfide gas generate;The step (3) in The litmus red test paper of separating obtained bacterial strain becomes blue, then proves there is ammonia generation.
More preferably, the step (2) in separating obtained bacterial strain without hydrogen sulfide gas generate, illustrate the taken city of the method Without the bacterial strain for producing hydrogen sulfide gas in city river water sample;The step (3) in separating obtained bacterial strain have ammonia generation, illustrate institute Stating has the bacterial strain for producing ammonia in the taken urban river water sample of method, illustrate in the smelly water body of urban river containing generation ammonia Bacterial strain.
Comprehensive apparently the method for the present invention can inhibit urban river strain growth, reduce ammonia-nitrogen content, and control ammonia generates, Effectively eliminate urban river stink.And the method for the present invention is at low cost, and operating method is simple, it will not cause damages to environment, safety Environmental protection.
The Costco Wholesale table of each preservative used in 4 the method for the present invention of table
Type Unit price (g/ member)
Sodium benzoate 0.32
Dehydroactic acid sodium 0.98
60% sodium lactate 0.3
Although disclosing the embodiment of the present invention for the purpose of illustration, it will be appreciated by those skilled in the art that: not Be detached from the present invention and spirit and scope of the appended claims in, various substitutions, changes and modifications be all it is possible, therefore, this The range of invention is not limited to the embodiment and disclosure of that.

Claims (10)

1. a kind of new method of urban river deodorization, it is characterised in that: described method includes following steps:
(1) river smelly water body in urban is taken out, and wherein urban river includes the industrial wastewater and sanitary sewage being discharged into, respectively The smelly water body of part industrial wastewater and the smelly water body of sanitary sewage are taken out, respectively from industrial wastewater and the smelly water body of sanitary sewage Screen isolated strains;
(2) whether verify separated bacterial strain has a stink generation, water body it is smelly including sulfur-bearing, nitrogen organic decompose the hydrogen sulfide overflowed, Caused by ammonia;And ammonia nitrogen refer in water in the form of free ammonia and ammonium ion existing for nitrogen, as long as therefore reducing ammonia nitrogen in water body and containing Amount reduces free ammonia, ammonia concentration, just can control stink generation;
(3) inhibit to produce smelly strain growth by adjusting the means that different pH, addition different preservatives and the two combine, reduce ammonia nitrogen Content, control stink generate.
2. urban river deodorization new method according to claim 1, it is characterised in that: specific step is as follows:
(1) screen isolated strains: screening and separation to bacterial strain are realized by cultivating on solid plate;
Screening and separation that YPD and LB solid medium carries out bacterial strain are chosen, the smelly water body of industrial wastewater and sanitary sewage are sent out Smelly water body dilutes 10 respectively-1With 10-2Two gradients take 200ul sample liquid difference even spread to two kinds not to be diluted to compare On culture medium, 48h is cultivated at 30 DEG C and 37 DEG C respectively later;
The secondary culture on corresponding culture medium respectively by obtained different strains, after obtaining single colonie, in respective liquid culture After being cultivated in base, with glycerol cold storage in -80 DEG C of refrigerators, for use;
(2) hydrogen sulfide verifying is produced: by isolated strain inoculated into corresponding fluid nutrient medium, by corresponding conditions culture 24 ~48h prepares bacteria suspension;Under aseptic condition, it is inoculated in produces H respectively2S is verified on slant medium, to ensure the accurate of result Property, use sterile water to be inoculated in sterile water as compareing under aseptic condition and produce H2S is verified on slant medium, constant temperature incubation, is seen Examine whether bacterial strain generates hydrogen sulfide gas;
Produce ammonia verifying: by isolated strain inoculated into corresponding fluid nutrient medium, by corresponding conditions culture 24~ 48h prepares bacteria suspension, under aseptic condition, takes 1 × 10 respectively6A/ml bacterium is transferred in respective liquid culture medium, and wherein liquid is trained Feeding base is placed in 50ml triangular flask, is put into wet litmus red test paper, corresponding revolving speed culture 24 in the envelope bottle film of triangular flask Whether~48h, as a control group with sterile water, observation litmus red test paper become blue, if becoming blue, prove separating obtained bacterial strain Generate ammonia;
(4) strain growth is inhibited, and control stink generates:
Ammonia-nitrogen content in water body is reduced, reduces free ammonia, ammonia concentration: under aseptic condition, by isolated bacterial strain corresponding Fluid nutrient medium in carry out 24~48h of culture, prepare bacteria suspension, take 1 × 106A/ml bacterium is transferred respectively into pH difference, addition In the fluid nutrient medium of different preservatives, 150r/min, 37 DEG C of constant temperature incubations to stationary phase measure its absorbance value and ammonia later Nitrogen content observes litmus red test paper discoloration, is inhibited and produces smelly strain growth, reduces ammonia-nitrogen content, and control stink produces The means that raw different pH, addition different preservatives and the two combines can carry out deodorization to urban river.
3. urban river deodorization new method according to claim 2, it is characterised in that: the step (4) in difference pH for for 3,4,5,6,7,8;The different preservatives and its whole mass concentration of addition are respectively 0.25% sodium benzoate, 0.05% dehydrogenation second Sour sodium, 3% sodium lactate.
4. urban river deodorization new method according to claim 3, it is characterised in that: the step (4) in difference pH, add The means for adding different preservatives and the two to combine are as follows:
When adjusting the pH≤4 of smelly water body, one or more of 3 kinds of preservatives are added;When the smelly water body of adjusting When pH=5, the sodium benzoate that whole mass concentration is 0.25% is added;
Alternatively, adding one or more of 3 kinds of preservatives when adjusting the pH≤3 of smelly water body;When the smelly water of adjusting When the pH of body≤4, the sodium benzoate that whole mass concentration is 0.25% and/or the dehydroactic acid that whole mass concentration is 0.05% are added Sodium;When adjusting the pH≤5 of smelly water body, the sodium benzoate that whole mass concentration is 0.25% is added.
5. urban river deodorization new method according to claim 2, it is characterised in that: the step (2) in H2S verifies inclined-plane Culture medium the preparation method is as follows: phenol red 0.0025g is added in every 100ml, ferrous sulfate 0.02g, tryptone 1g, yeast mention Take object 0.5g, sodium chloride 1g, agar 1.2g.
6. urban river deodorization new method according to claim 2, it is characterised in that: the step (4) in the different liquid of pH Body culture medium the preparation method is as follows: adjusting fluid nutrient medium pH respectively is 3,4,5,6,7,8, and place it in high-pressure sterilizing pot In in 115 DEG C of sterilizing 20min.
7. urban river deodorization new method according to claim 5, it is characterised in that: the liquid of the addition different preservatives Body culture medium the preparation method is as follows:
1. 0.25% sodium benzoate Liquid Culture based sols: under sterile conditions, first weigh sodium benzoate 0.25g, and by its It is dissolved in the sterilized fluid nutrient medium of 100ml, shakes up, be filtered later with sterilised membrane filter device and degerming, be placed in 4 DEG C of refrigerators It saves backup;
2. 0.05% dehydroactic acid sodium Liquid Culture based sols: aseptically, first weighing dehydroactic acid sodium 0.05g, then will It is dissolved in the sterilized fluid nutrient medium of 100ml, shakes up, and is filtered simultaneously degerming with sterilised membrane filter device later, is placed in 4 DEG C of ice Case saves backup;
3. 3% sodium lactate Liquid Culture based sols: under aseptic condition, drawing the lactic acid that 3ml mass concentration is 60% with liquid-transfering gun Sodium solution, and be added into the sterilized fluid nutrient medium of 100ml, it shakes up, is filtered and is removed with sterilised membrane filter device later Bacterium is placed in 4 DEG C of refrigerator and saves backup.
8. urban river deodorization new method according to claim 2, it is characterised in that: the step (4) in take 1 × 106A/ Ml bacterium is transferred respectively, and pH is different, the pH in fluid nutrient mediums of addition different preservatives is different, addition different preservatives Fluid nutrient medium are as follows: 6 kinds of different pH value are combined with 3 kinds of different preservatives respectively, the fluid nutrient medium being prepared.
9. urban river deodorization new method according to claim 2, it is characterised in that: the step (1) in from industrial wastewater Isolated 5 plants of bacterium are screened in smelly water body, from sanitary sewage in water body isolated 4 plants of bacterium;The step (2) in point It is generated without hydrogen sulfide gas from obtained strains;The step (3) in the litmus red test paper of separating obtained bacterial strain become blue, then Proof has ammonia generation.
10. according to the described in any item urban river deodorization new methods of claim 2 to 9, it is characterised in that: the step (2) in Separating obtained bacterial strain is generated without hydrogen sulfide gas, is illustrated in the taken urban river water sample of the method without production hydrogen sulfide gas Bacterial strain;The step (3) in separating obtained bacterial strain have ammonia generation, illustrate there is production in the taken urban river water sample of the method The bacterial strain of ammonia illustrates in the smelly water body of urban river containing the bacterial strain for generating ammonia.
CN201811195699.6A 2018-10-15 2018-10-15 A kind of new method of urban river deodorization Pending CN109467140A (en)

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