CN109430060A - A kind of cultural method extending Kiwifruit Tissue Culture seedling storage life - Google Patents

A kind of cultural method extending Kiwifruit Tissue Culture seedling storage life Download PDF

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Publication number
CN109430060A
CN109430060A CN201811603447.2A CN201811603447A CN109430060A CN 109430060 A CN109430060 A CN 109430060A CN 201811603447 A CN201811603447 A CN 201811603447A CN 109430060 A CN109430060 A CN 109430060A
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China
Prior art keywords
tissue culture
culture seedling
kiwifruit
storage life
seedling
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CN201811603447.2A
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Chinese (zh)
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CN109430060B (en
Inventor
高敏霞
陈义挺
冯新
赖瑞联
陈文光
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Pomology Research Institute Fujian Academy of Agricultural Sciences
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Pomology Research Institute Fujian Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants

Abstract

The invention discloses a kind of cultural methods for extending Kiwifruit Tissue Culture seedling storage life, it is that a certain amount of sucrose is added on the basis of Kiwifruit Tissue Culture seedling tradition subculture medium, effectively to extend the holding time of tissue-cultured seedling, solve the problems, such as that current Kiwifruit Tissue Culture seedling storage life is short.The preservation of Kiwifruit Tissue Culture seedling is carried out by the method for the present invention, generally can be by delayed growth 4-5 months of tissue-cultured seedling, and tissue culture shoot survival percent is up to 80% or more.

Description

A kind of cultural method extending Kiwifruit Tissue Culture seedling storage life
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of training for extending Kiwifruit Tissue Culture seedling storage life The method of supporting.
Background technique
Kiwi berry nutritious abundant, containing ingredients such as multivitamin, fat and protein, wherein Vitamin C content is 100 Mg-400 mg/100 g, significantly larger than citrus, pears and apple are known as the title of " king of Vc ".Kiwi berry other than edible value, It spends beautiful fragrance, with higher ornamental value.China is the center of Actinidia distribution, and germ plasm resource is abundant, divides Cloth is extensive, but is chronically at wild or semi-wild state, and due to the deterioration of ecological environment, some kind has been in condition in imminent danger, because This needs to collect and save.
Common store method has collection seed, branch and nursery stock to establish resource garden.Resource garden is established with occupied space Greatly, the disadvantages of recruitment is big, fees of maintenance are high.The appearance of plant tissue culture technique, development and perfect, be plant germplasm resource from Body preservation provides possibility.Plantlet in vitro with sterile culture, occupy little space, recruitment and fees of maintenance are low, convenient for each department germplasm It the advantages that exchange and the rescue of endangered species and quickly breeding, has been applied in various plants (peach, pears, lichee etc.).Cause This, saves Germplasm Resources in Genera Actinidia using plant tissue culture technique, it appears very necessary and urgent.
During Vitro Plant saves germplasm, medium nutrient content, moisture are gradually depleted, when one section Between it is necessary to which culture is inoculated on new culture medium, to guarantee normal growth.The general 1 month subculture one of Kiwifruit Tissue Culture seedling Secondary, longest can be reserved for 2 months or so, and frequent subculture needs to spend a large amount of human and material resources and time;Meanwhile with after generation The variation probability of several increases, culture materials also increases, and is unfavorable for the preservation of original germplasm.
Summary of the invention
The purpose of the present invention is to provide a kind of cultural methods for extending Kiwifruit Tissue Culture seedling storage life, to utilize plant group The Plantlet in vitro offer technical support that culture technique carries out Germplasm Resources in Genera Actinidia is provided.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of cultural method extending Kiwifruit Tissue Culture seedling storage life comprising following steps:
(1) pollution-free, robust growth Kiwifruit Tissue Culture seedling is inoculated in dedicated subculture medium;
(2) it is protected in 25 ± 1 DEG C of cultivation temperature, the culturing room of light application time 12 h/d, intensity of illumination 1500-2000 lx It deposits;
(3) when tissue culture seedling leaf to be found dries up, the dedicated subculture medium that more renews.
The dedicated subculture medium is MS+6-BA 1.0-2.0 mg/L+NAA 0.5-1.0 mg/L+GA3 0.1 mg/ 7 g/L+ sucrose 15-20 g/L of L+ agar.
The beneficial effects of the present invention are:
(1) present invention is effectively slowed down the growth of tissue-cultured seedling, is reduced nutrition and disappeared by the concentration of sucrose in defined medium The subculture interval time of Kiwifruit Tissue Culture seedling was extended to 4-5 months by 2 months, greatly reduces the work of subinoculation by consumption Amount, and tissue culture shoot survival percent is up to 80% or more, while also reducing variation probability.
(2) present invention has the characteristics that simple to operation, safe and reliable, will not generate side effect, method to culture materials It is simple and effective, have many advantages, such as that strong operability, application value are high.
Influence of 1 different sucrose of table to Kiwifruit Tissue Culture seedling storage life
By 1 result of table as it can be seen that one times of prolonged shelf life of Kiwifruit Tissue Culture seedling can be made using the present invention, and save tissue culture cost.
Specific embodiment
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention Technical solution is described further, but the present invention is not limited only to this.
Embodiment 1
A kind of cultural method extending Kiwifruit Tissue Culture seedling storage life, comprising the following steps:
(1) 1.0 mg/L+NAA of MS+6-BA, 0.5 mg/L+GA is pressed30.1 mg/L+ agar 7g/L+ sucrose, 15 g/L prepares special Use subculture medium;PH to 6.0 is adjusted before medium sterilization;
(2) pollution-free, robust growth Kiwifruit Tissue Culture seedling is selected to be inoculated in above-mentioned culture medium;
(3) the Kiwifruit Tissue Culture seedling after being inoculated with is placed in 25 ± 1 DEG C of cultivation temperature, light application time 12 h/d, intensity of illumination 1500- It is saved in the culturing room of 2000 lx;
(4) tissue-cultured seedling of retarding of growing can be kept 5 months, when during which finding that tissue culture seedling leaf dries up, more be renewed in time Culture medium.The survival rate of tissue-cultured seedling after culture is up to 86%.
Embodiment 2
A kind of cultural method extending Kiwifruit Tissue Culture seedling storage life, comprising the following steps:
(1) 1.0 mg/L+NAA of MS+6-BA, 1.0 mg/L+GA is pressed30.1 mg/L+ agar 7g/L+ sucrose, 20 g/L is prepared Dedicated subculture medium;PH to 6.0 is adjusted before medium sterilization;
(2) pollution-free, robust growth Kiwifruit Tissue Culture seedling is selected to be inoculated in above-mentioned culture medium;
(3) the Kiwifruit Tissue Culture seedling after being inoculated with is placed in 25 ± 1 DEG C of cultivation temperature, light application time 12 h/d, intensity of illumination 1500- It is saved in the culturing room of 2000 lx;
(4) tissue-cultured seedling of retarding of growing can be kept 4.5 months, when during which finding that tissue culture seedling leaf dries up, more be renewed in time Culture medium.The survival rate of tissue-cultured seedling after culture is up to 83%.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, is all covered by the present invention.

Claims (2)

1. a kind of cultural method for extending Kiwifruit Tissue Culture seedling storage life, it is characterised in that: the following steps are included:
(1) pollution-free, robust growth Kiwifruit Tissue Culture seedling is inoculated in dedicated subculture medium;
(2) it is protected in 25 ± 1 DEG C of cultivation temperature, the culturing room of light application time 12 h/d, intensity of illumination 1500-2000 lx It deposits;
(3) when tissue culture seedling leaf to be found dries up, the dedicated subculture medium that more renews.
2. extending the cultural method of Kiwifruit Tissue Culture seedling storage life according to claim 1, it is characterised in that: described dedicated Subculture medium is MS+6-BA 1.0-2.0 mg/L+NAA 0.5-1.0 mg/L+GA30.1 mg/L+ agar, 7 g/L+ sucrose 15-20 g/L。
CN201811603447.2A 2018-12-26 2018-12-26 Culture method for prolonging storage life of kiwi fruit tissue culture seedlings Active CN109430060B (en)

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CN201811603447.2A CN109430060B (en) 2018-12-26 2018-12-26 Culture method for prolonging storage life of kiwi fruit tissue culture seedlings

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CN201811603447.2A CN109430060B (en) 2018-12-26 2018-12-26 Culture method for prolonging storage life of kiwi fruit tissue culture seedlings

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CN109430060A true CN109430060A (en) 2019-03-08
CN109430060B CN109430060B (en) 2022-06-21

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Cited By (1)

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CN112335548A (en) * 2020-11-12 2021-02-09 福建省农业科学院果树研究所 In-vitro conservation method of kiwi fruit germplasm resources

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Publication number Priority date Publication date Assignee Title
CN112335548A (en) * 2020-11-12 2021-02-09 福建省农业科学院果树研究所 In-vitro conservation method of kiwi fruit germplasm resources

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