CN109406247A - Discharger for micro slide experiment liquid - Google Patents

Discharger for micro slide experiment liquid Download PDF

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Publication number
CN109406247A
CN109406247A CN201811261453.4A CN201811261453A CN109406247A CN 109406247 A CN109406247 A CN 109406247A CN 201811261453 A CN201811261453 A CN 201811261453A CN 109406247 A CN109406247 A CN 109406247A
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CN
China
Prior art keywords
drain
liquid
side wall
micro slide
mesospore
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811261453.4A
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Chinese (zh)
Inventor
陈鑫
王成成
李思勇
余向东
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According To United States Rock Hangzhou Medical Science And Technology Co Ltd
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According To United States Rock Hangzhou Medical Science And Technology Co Ltd
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Priority to CN201811261453.4A priority Critical patent/CN109406247A/en
Publication of CN109406247A publication Critical patent/CN109406247A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The present invention relates to a kind of dischargers for micro slide experiment liquid, its structure includes drainage region, the drainage region includes drain antetheca, drain side wall and leakage fluid dram, the drain side wall is arranged in drainage region two sides, the drain antetheca connects two sides drain side wall, and the leakage fluid dram is limited by drain side wall and drain antetheca;Protrusion is equipped with outside contact portion on the drain side wall, and the outside contact portion is in contact with micro slide side simultaneously;The upper surface of the drain antetheca is equipped with plane and lower liquid portion, the plane are connected to the upper surface of drain side wall, and drain direction of the lower liquid portion from plane to leakage fluid dram extends;When drainage region and micro slide cooperate, the upper surface of micro slide and drain side wall, there are gaps for interplanar.When the present invention can contact with contact portion in micro slide or is separate, the device of accurate direct experiment liquid discharge;And the experiment liquid velocity of discharge of the device is faster;It heats during heating also more uniformly.

Description

Discharger for micro slide experiment liquid
Technical field
The present invention relates to a kind of liquid discharge device, especially a kind of discharger for micro slide experiment liquid.
Background technique
In Bioexperiment, sample passes through pre-processing, then by by a series of dyeing and remaining processing, makes experimenter can be with The case where clearly observing sample.Common processing includes the series of steps such as pretreatment and dyeing, uses people under regular situation Work operation, sample tissue is placed on micro slide, by dewaxing, reparation, dyeing and dehydration and etc., above-mentioned steps need artificial Real-time liquid feeding, removal waste fluid are that direct water rinses plus gets rid of slide.This process takes a long time, and needs personal monitoring, therefore occurs It can apply to automation means and carry out tissue treatment, the discharge of experiment liquid is carried out in automation process, and then generate special Discharger for the experiment of this kind of automatic biological.
A kind of existing discharger for micro slide experiment liquid, it is raised by being arranged on leakage fluid dram two sidewalls, it should One effect of protrusion is positioning micro slide, another effect is to play attraction and drainage effect by the protrusion, makes to test liquid Flow into leakage fluid dram along side wall, but the discharger has the following problems: when testing liquid and projection contacts, which can not Side of the liquid stream to leakage fluid dram or the side far from leakage fluid dram will be tested by controlling well, therefore part Experiment liquid is easy from row Outflow on the outside of device out, can pollute other structures, if things go on like this, can damage to instrument.
Chinese patent disclosure CN108051270A discloses a kind of discharger for micro slide reaction solution, passes through Drain mesospore is set, and protrusion is equipped with intermediate contact portion on drain mesospore, the height of projection of intermediate contact portion is greater than to be flanked outside Contact portion is that intermediate contact portion elder generation contact liq plays drainage effect, and trace is had after liquid flow, in this way to next time Liquid just generate very strong attraction, further can concentrate liquid in centre like that and reduce liquid and contacted with outside contact portion A possibility that, avoid liquid from polluting other structures.But above-mentioned discharger there is problems: 1, when micro slide is indirect in When contact portion contacts, even if intermediate contact portion is close to setting with micro slide, the back side that liquid is back to micro slide is also unavoidably tested, The experiment liquid of the reflux discharger can not be carried out effectively draining and make it into leakage fluid dram;2, in experimentation, loading Piece is moved and is left contact portion, and the discharger, which can not carry out effectively draining, at this time makes it into leakage fluid dram.
Summary of the invention
When can be contacted in micro slide with contact portion technical problem to be solved by the invention is to provide one kind or is separate, essence The device of true direct experiment liquid discharge;And the experiment liquid velocity of discharge of the device is faster;It heats during heating also more Uniformly.
The technical proposal adopted by the invention to solve the above technical problems is that:
A kind of discharger for micro slide experiment liquid, structure includes drainage region, before the drainage region includes drain Wall, drain side wall and leakage fluid dram, in drainage region two sides, the drain antetheca connects two sides drain side for the drain side wall setting Wall, the leakage fluid dram are limited by drain side wall and drain antetheca;Protrusion is equipped with outside contact portion on the drain side wall, described outer It flanks contact portion while being in contact with micro slide side;The upper surface of the drain antetheca is equipped with plane and lower liquid portion, described flat Face is connected to the upper surface of drain side wall, and drain direction of the lower liquid portion from plane to leakage fluid dram extends;When drainage region and carry When object piece cooperates, the upper surface of micro slide and drain side wall, there are gaps for interplanar.Liquid, which is drained, needs to construct a liquid Access, the displacement of liquid involved in Bioexperiment process and the discharge for being replaced liquid, the technical program, which refers to, accurately to help Liquid is replaced, and will be needed the liquid being replaced accurate while displacement and is fully discharged.During displacement, Liquid flows between cover plate and micro slide, can contact drainage with contact portion raised on drain side wall first and enter leakage fluid dram, but Due to the scrambling of liquid flowing, partially liq can be back to the back side of micro slide, due to the presence in gap, drain side wall Upper surface, plane are infiltrated, and due to the presence in lower liquid portion, the liquid of lower liquid portion aggregation can be prior to the upper surface of drain side wall It is discharged with flat liquid, and then plays the effect of drainage and attraction to the upper surface at the back side of micro slide, drain side wall, flat liquid Fruit, lower liquid portion to the drain direction of leakage fluid dram due to being extended, to guarantee all liquid energy for flowing back into the micro slide back side Accurately flow into leakage fluid dram.Similarly, the case where there are micro slides far from contact portion during Bioexperiment, experiment liquid discharge When due to capillary phenomenon presence, can the upper surface of drain side wall, plane in preferential wetting gap, it is then accurate by lower liquid portion Discharge.
Preferably, the range in the gap is 0.005-0.2mm.
Preferably, the lower liquid portion is the combination of following any structure or structure: inclined surface, cascaded surface, arcwall face, Chamfering.
It further, further include drain mesospore, the drain mesospore is arranged between drain side wall and connects with drain antetheca It connecing, protrusion is equipped with intermediate contact portion on an at least drain mesospore, and the height of projection of the intermediate contact portion is greater than outside contact portion, Among the outside contact portion contact portion simultaneously with one side contacts of micro slide;The lower liquid portion is at least connected with drain side wall or drain Mesospore;When drainage region and micro slide cooperate, there is also gaps between micro slide and the upper surface of drain mesospore.Since liquid flows Characteristic, the experiment liquid for needing to be discharged can be assembled in micro slide center end, therefore most of experiment liquid can be along intermediate contact portion It is flowed down along drain mesospore, and since drain mesospore is closer away from the liquid assembled in lower liquid portion, which is also easy to pass through Drain mesospore discharge, to collaborate with the liquid of intermediate contact portion discharge, play the role of attraction and accelerate discharge.
Preferably, the lower liquid portion is with drain mesospore, drain side wall by being connected smoothly.The company of being connected smoothly It connects and further helps in discharge liquid and collect and generate drainage effect, to pass through more quickly lower liquid portion and liquid is discharged.
Further, the plane is connected to the upper surface of drain mesospore, and the longitudinal length of the drain mesospore is less than row The longitudinal length of liquid side wall and the longitudinal length for being greater than lower liquid portion, drain mesospore lower surface form liquid pooling zone.Drain The setting of mesospore enables the experiment liquid flowed down from drain mesospore two sides to collect in mesospore lower surface, forms big drop and passes through gravity It is discharged between discharger, greatly accelerates the velocity of discharge of experiment liquid, so that liquid be avoided to assemble in the upper surface of device And the case where causing the part except leakage fluid dram to be discharged.
Preferably, wide above and below the drain mesospore or be gradually reduced along the downward face width in upper surface, the drain The width range of mesospore lower surface is 1-1.2mm.The shape of drain mesospore and the width selection of lower surface are conducive to two sides liquid Body collects.
Preferably, further including drain rear wall, the leakage fluid dram is limited by drain side wall, drain antetheca and drain rear wall.
It further, further include heating zone, the heating zone is connected to the front end of drainage region, the upper surface of the heating zone Upper surface than drain antetheca, drain side wall, drain mesospore is 0.005-0.2mm high.The setting of heating zone puts micro slide directly It sets and can produce above-mentioned gap on discharger.
Preferably, the heating zone includes metal shell and heating part, the heating part is fixed on metal shell.Benefit Existing full ceramic heat portion is replaced with the Thermal conductivity of metal shell, makes to heat in heating process more uniform.
The present invention compared with the existing technology has the following advantages that and effect: the technical program guarantee is all to flow back into micro slide The liquid at the back side can accurately flow into leakage fluid dram.Similarly, feelings during Bioexperiment there are micro slide far from contact portion Condition tests presence when liquid is discharged due to capillary phenomenon, the upper surface of drain side wall, plane in the preferential wetting gap of meeting, then It is precisely discharged by lower liquid portion;The setting of drain mesospore is further ensured that experiment liquid enters leakage fluid dram, and promotes lower liquid portion and row The experiment liquid of liquid mesospore two sides can be flowed down quickly, experiment liquid collect in mesospore lower surface, formed big drop by gravity from Be discharged between discharger, greatly accelerate experiment liquid the velocity of discharge, thus avoid liquid device upper surface aggregation and The case where causing the part except leakage fluid dram to be discharged;Existing full ceramics are replaced to add using the Thermal conductivity of metal shell Hot portion makes to heat in heating process more uniform.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention without any creative labor, may be used also for those of ordinary skill in the art To obtain other drawings based on these drawings.
Fig. 1 is the structural schematic diagram of embodiment 1.
Fig. 2 is the cross-sectional view in the face A-A of embodiment 1.
Fig. 3 is the cross-sectional view in the face B-B of embodiment 1.
Fig. 4 is that embodiment 1 and micro slide cooperate schematic diagram.
Fig. 5 is the enlarged drawing of the drainage region Fig. 4.
Fig. 6 is the drain schematic diagram of the drainage region Fig. 4.
Fig. 7 is the structural schematic diagram of 1 drainage region of embodiment.
Fig. 8 is the rearview of embodiment 1.
Fig. 9 is the cross-sectional view in the face A-A of embodiment 2.
Figure 10 is the cross-sectional view in the face A-A of embodiment 3.
Figure 11 is the cross-sectional view in the face A-A of embodiment 4.
Figure 12 is the structural schematic diagram of embodiment 5.
Label declaration:
1 drain antetheca of drainage region, 11 plane 111
112 drain side wall of lower liquid portion, 12 outside contact portion 121
The intermediate 131 drain mesospore lower surface 132 of contact portion of drain mesospore 13
14 leakage fluid dram of drain rear wall, 15 gap 16
2 metal shell upper surface of heating zone, 21 heating part 22
Micro slide 3
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, following embodiment be explanation of the invention and The invention is not limited to following embodiments.
Embodiment 1:
As shown in Figure 1, the present embodiment is made of drainage region 1 and heating zone 2, wherein drainage region 1 includes drain antetheca 11, two Drain side wall 12 and drain rear wall 14, drain antetheca 11, two drain side walls 12 surround a central through hole out with drain rear wall 14, should Through-hole is leakage fluid dram 15,15 middle section of leakage fluid dram is provided with drain mesospore 13, drain mesospore 13 is parallel to two drain sides Wall 12 is arranged, wherein protrusion has outside contact portion 121 on two drain side walls 12, protrusion has intermediate contact portion on drain mesospore 13 131, outside contact portion 121 is arranged in 15 middle part of leakage fluid dram close to the position of drain antetheca 11 with intermediate contact portion 131 and is located at On same straight line, in conjunction with Fig. 3 and Fig. 5, wherein intermediate contact portion 131 is higher by outside contact portion 121, and three is below micro slide 3 thickness, it is 0.5mm that wherein the present embodiment China and foreign countries, which flank the height of contact portion 121, and the height of intermediate contact portion 131 is 0.7mm, Three can be with 3 side snug fit of micro slide.In conjunction with Fig. 1,2 and 7, drain mesospore 13 is made of plane 111 and lower liquid portion 112, Wherein plane 111 is flushed and is connected to the upper surface of two drain side walls 12 and drain mesospore 13, and lower liquid portion 112 inclines from plane 111 Extend below oblique leakage fluid dram 15 and be arranged between drain side wall 12 and drain rear wall 14, tilt angle is 45 °, chamfer length Two sides for 1mm, 112 inclined surface of lower liquid portion are connect with drain side wall 12 and drain mesospore 13 by smooth cambered surface.Such as Fig. 2 institute Show, longitudinal height of drain mesospore 13 is shorter than two drain side walls 12, and the width of drain mesospore lower surface 132 is set as 1.2mm;Add The front of drain antetheca 11 is arranged in hot-zone 2, and the front of drain antetheca 11 connects heating zone 2 after extending a U-shaped sump pit, such as Shown in Fig. 8, heating zone 2 is made of metal shell and heating part 22, and a recess is provided on metal shell lower surface, makes heating part 22 can be embedded in make heat quickly conduct in metal shell, and wherein metal shell uses the good material of heating conduction , such as copper, aluminium, can also prevent from corroding while thermally conductive using alloy, 22 useful ceramics piece of heating part, wherein metal-back Body upper surface 21 is higher by 12 upper surface 0.02mm of drain antetheca 11 and two drain side walls, thus when micro slide 3 is erected at metal-back When body upper surface 21, there are gap 16 (as shown in Figure 5) for micro slide 3 and drain antetheca 11 and two drain side walls, 12 upper surface part
As shown in fig. 6, when micro slide 3 and the present embodiment cooperation drain when, due to experiment liquid itself feature and in indirectly The attraction of contact portion 131, experiment liquid are mainly concentrated in middle section and by intermediate contact portion 131 towards the two sides of drain mesospore 13 It flows down, as shown in figure 3, can collect to form liquid and collect in lower surface when liquid stream to drain mesospore lower surface 132 is tested in two sides Area, two sides collect after liquid due to quality increase can by gravity directly from leakage fluid dram 15 be discharged, in order to facilitate the remittance of liquid Collection, the structure that width is sequentially reduced from top to bottom can be set into drain mesospore 13, or arc is arranged in drain mesospore lower surface 132 Shape face.As shown in Fig. 4 to 7, since characteristics of liquids can generate liquid reflux or in the case where micro slide 3 is far from contact portion, by In micro slide 3, there are the gaps of 0.02mm with 1 front upper surface of drainage region, and by capillary phenomenon, the portion of upper surface is (in Fig. 7 Oblique line) it can preferentially be infiltrated by experiment liquid, wherein liquid can be moved to the lower liquid portion 112 that be connected to plane 111, and from lower liquid portion 112 are discharged to leakage fluid dram 15.
Embodiment 2:
The present embodiment is substantially the same manner as Example 1, and difference is that lower liquid portion 112 is made of a chamfering, as shown in figure 9, It is not easy to go out to assemble in lower liquid portion 112 using lower 112 liquid of liquid portion of chamfering structure, experiment liquid is in infiltration drain side wall 12, drain Behind the upper surface of mesospore 13 and plane 111,11 side of the drain antetheca discharge that can be quickly connected from lower liquid portion 112, thus Be not easy to overflow from saturated surface, but farther out due to the drain runner with drain side wall 12, drain mesospore 13, it is more difficult to formed confluence or Biggish water droplet, whole flow velocity have no promotion.In view of the above circumstances, due to lower liquid portion 112 and drain side wall 12, drain mesospore 13 cooperation is less, and the lower liquid portion 112 of chamfering structure can not also be connect with drain side wall 12, drain mesospore 13.
Embodiment 3
The present embodiment is substantially the same manner as Example 1, and difference is that lower liquid portion 112 is made of the cambered surface of an indent, such as schemes Just the opposite using the lower liquid portion 112 and 2 chamfering structure property of embodiment of inner concave arc surface shown in 10, inner concave arc surface is easier poly- Collection experiment liquid, so that experiment liquid be made to contact with drain side wall 12, drain mesospore 13 and along drain side wall 12,13 row of drain mesospore Out, thus be more readily formed confluence or biggish water droplet, the discharge flow velocity for testing liquid be apparently higher than embodiment 2.
Embodiment 4
The present embodiment is substantially the same manner as Example 1, and difference is that lower liquid portion 112 is a cascaded surface, as shown in figure 11, knot Structure is made of a L-type face and two chamferings, wherein the wherein one side of upper chamfering connection plane 111 and L-type face, lower chamfering connection 11 side of L-type face another side and drain antetheca, the structure combine the design feature of embodiment 2 and embodiment 3, and upper chamfering makes reality Test liquid be easier to enter L-type face and be not easy from saturated surface overflow, into L-type face after part Experiment liquid aggregation to drain side wall 12, drain mesospore 13 is contacted and is discharged along drain side wall 12, drain mesospore 13;And if the lifting rate short time increasing of experiment liquid Greatly, it when L-type face liquid accumulation is excessive, can be expelled directly out by lower chamfering from 11 side of drain antetheca.
Embodiment 5
The present embodiment is substantially the same manner as Example 1, and difference is to be not provided with drain mesospore 13, and lower liquid portion 112 is integral, As shown in figure 12, a possibility which is discharged on the outside of drain side wall 12 there is also experiment liquid, but since gap 16 is under The presence in liquid portion 112, the experiment liquid not being discharged on the inside of drain side wall 12 are possible to enter the infiltration of gap 16 row because of capillary phenomenon 12 upper surface of liquid side wall and plane 111, to reduce a possibility that leaking out outward.
The present invention can also realize that the tilt angle in following liquid portion is 60 ° using the technical solution of other numerical value;It gap can Using 0 any value is greater than, as long as the effect for attracting liquid is able to achieve, wherein being preferred with 0.005-0.2mm;Similarly Any value greater than 0 can also be used in the lower surface of the range of drain mesospore, wherein being preferred with 1-1.2mm.
In addition, it should be noted that, the specific embodiments described in this specification, the shape of parts and components are named Title etc. can be different.The equivalent or simple change that all structure, feature and principles described according to the invention patent design are done, is wrapped It includes in the scope of protection of the patent of the present invention.Those skilled in the art can be to described specific implementation Example is done various modifications or additions or is substituted in a similar manner, and without departing from structure of the invention or surmounts this Range as defined in the claims, is within the scope of protection of the invention.

Claims (10)

1. a kind of discharger for micro slide experiment liquid, it is characterised in that: including drainage region, the drainage region includes drain Antetheca, drain side wall and leakage fluid dram, in drainage region two sides, the drain antetheca connects two sides drain side for the drain side wall setting Wall, the leakage fluid dram are limited by drain side wall and drain antetheca;Protrusion is equipped with outside contact portion on the drain side wall, described outer It flanks contact portion while being in contact with micro slide side;The upper surface of the drain antetheca is equipped with plane and lower liquid portion, described flat Face is connected to the upper surface of drain side wall, and drain direction of the lower liquid portion from plane to leakage fluid dram extends;When drainage region and carry When object piece cooperates, the upper surface of micro slide and drain side wall, there are gaps for interplanar.
2. the discharger according to claim 1 for micro slide experiment liquid, it is characterised in that: the range in the gap For 0.005-0.2mm.
3. it is according to claim 1 for micro slide experiment liquid discharger, it is characterised in that: the lower liquid portion be with The combination of lower any structure or structure: inclined surface, cascaded surface, arcwall face, chamfering.
4. the discharger according to any one of claims 1 to 3 for micro slide experiment liquid, it is characterised in that: further include Drain mesospore, the drain mesospore is arranged between drain side wall and connect with drain antetheca, raised on an at least drain mesospore Equipped with intermediate contact portion, the height of projection of the intermediate contact portion is greater than outside contact portion, contact among the outside contact portion Portion simultaneously with one side contacts of micro slide;The lower liquid portion is at least connected with drain side wall or drain mesospore;When drainage region and micro slide When cooperation, there is also gaps between micro slide and the upper surface of drain mesospore.
5. the discharger according to claim 4 for micro slide experiment liquid, it is characterised in that: the lower liquid portion and row Liquid mesospore, drain side wall are by being connected smoothly.
6. the discharger according to claim 4 for micro slide experiment liquid, it is characterised in that: the plane and drain The upper surface of mesospore is connected to, and the longitudinal length of the drain mesospore is less than the longitudinal length of drain side wall and is greater than the vertical of lower liquid portion To length, drain mesospore lower surface forms liquid pooling zone.
7. the discharger according to claim 6 for micro slide experiment liquid, it is characterised in that: on the drain mesospore Under it is wide or be gradually reduced along the downward face width in upper surface, the width range of drain mesospore lower surface is 1-1.2mm.
8. the discharger according to claim 1 for micro slide experiment liquid, it is characterised in that: after further including drain Wall, the leakage fluid dram are limited by drain side wall, drain antetheca and drain rear wall.
9. the discharger according to claim 2 for micro slide experiment liquid, it is characterised in that: it further include heating zone, The heating zone is connected to the front end of drainage region, and the upper surface of the heating zone is than drain antetheca, drain side wall, drain mesospore The high 0.005-0.2mm in upper surface.
10. the discharger according to claim 9 for micro slide experiment liquid, it is characterised in that: the heating zone packet Metal shell and heating part are included, the heating part is fixed on metal shell.
CN201811261453.4A 2018-10-26 2018-10-26 Discharger for micro slide experiment liquid Pending CN109406247A (en)

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CN111562164A (en) * 2020-05-15 2020-08-21 河南赛诺特生物技术有限公司 Liquid cover film

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