CN109288957A - Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart - Google Patents

Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart Download PDF

Info

Publication number
CN109288957A
CN109288957A CN201811297756.1A CN201811297756A CN109288957A CN 109288957 A CN109288957 A CN 109288957A CN 201811297756 A CN201811297756 A CN 201811297756A CN 109288957 A CN109288957 A CN 109288957A
Authority
CN
China
Prior art keywords
acid
extract
value
easypro
invigorating heart
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811297756.1A
Other languages
Chinese (zh)
Inventor
安怀略
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guizhou Xinbang Pharmaceutical Co Ltd
Original Assignee
Guizhou Xinbang Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guizhou Xinbang Pharmaceutical Co Ltd filed Critical Guizhou Xinbang Pharmaceutical Co Ltd
Priority to CN201811297756.1A priority Critical patent/CN109288957A/en
Publication of CN109288957A publication Critical patent/CN109288957A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/57—Magnoliaceae (Magnolia family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/79—Schisandraceae (Schisandra family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8968—Ophiopogon (Lilyturf)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to a kind of methods and products thereof that alkali extraction and acid precipitation extracts effective component in the easypro composition of invigorating heart, it is specifically raw material by ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn, it is prepared using alkali extraction and acid precipitation extraction, this method can be improved the recovery rate of effective component in the easypro composition of invigorating heart, to reduce the dose of the easypro preparation of invigorating heart, therapeutic effect is more preferable；And extracting method is highly-safe.

Description

Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart
Technical field
The present invention relates to a kind of methods and products thereof that alkali extraction and acid precipitation extracts effective component in the easypro composition of invigorating heart, belong to Invigorating heart is relaxed preparation preparation technical field.
Background technique
Invigorating heart relaxes preparation with Yiqi and vein recovery, activating microcirculation and removing stasis medicinal, nourishing Yin and promoting production of body fluid.For deficiency of both qi and yin, palpitaition knotted pulse is uncomfortable in chest Not easypro, pectoralgia and coronary disease and angina pectoris, according to the literature, the number that cardiovascular and cerebrovascular disease is died of in the annual whole world is more than 2000 Ten thousand, China nearly 400,000.Chinese medicine has accumulated clinical experience abundant in terms of the diagnosis and treatment of the disease, and Chinese medicinal formulae is in treatment cardiovascular and cerebrovascular There is unique advantage in terms of disease.Pectoralgia and coronary disease and angina pectoris category Chinese medicine " thoracic obstruction " scope.In its cause of disease of thoracic obstruction and cold-evil Invade, be improper diet, disorder of emotion, body deficiency due to old age etc. it is related, the turbid congestion of the stagnation of the heart blood, phlegm, phlegm stagnancy, the heart can be divided by syndrome The diseases type such as kidney-yin deficiency, deficiency of both qi and yin, deficient and weak YANG QI." heart vessel blockage stasis " is the principle pathological mechanism of this disease, clinical actual situation folder mostly It is miscellaneous, be in the time of this void table reality, treat this disease advocate to based on mending to lead to for principle, benefit, main tonifying Qi is negative, it is intended to adjust internal organs Qi and blood is contained partially to decline partially, with the card for void of effecting a permanent cure, leads to, and main promoting blood circulation activates yang, it is intended that the numbness resistance for network of promoting blood circulation is declared, with the marquis for reality for the treatment of the symptoms.
Currently, invigorating heart is relaxed, preparation has tablet, capsule etc. for the dosage form of selection of clinical.Ginseng, Radix Ophiopogonis, the five tastes in prescription Son, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn are rich in a variety of effective ingredients, and relax the ingredient master played a role in preparation in invigorating heart Have: terpene, polysaccharide, flavonoids and saponin component.
Currently, invigorating heart is relaxed, preparation mostly uses greatly traditional preparation method to prepare, such as water extraction, alcohol extracting method.Though water extraction Right extraction process is simple and convenient to operate, but there are many effective ingredients due to needing to extract, is obtained by water extraction Effective ingredient is essentially crude extract, and recovery rate generally only has 75%, and the dose for not only resulting in patient in this way is larger, and And it also will have a direct impact on the quality and clinical efficacy of product.And although alcohol extracting method recovery rate is higher, alcohol extracting method needs to consume Alcohol solvent, so that increased costs；In addition, from the safety of production, organic solvent has inflammability, and excessive has Solvent is harmful to the body of operator, should reduce the use of organic solvent to the greatest extent.
Summary of the invention
The object of the present invention is to provide a kind of alkali extraction and acid precipitation extract invigorating heart relax composition in effective component method and Its product, can be improved the recovery rate of effective component in the easypro composition of invigorating heart, to reduce the dose of the easypro preparation of invigorating heart, treatment Effect is more preferable；And extracting method is highly-safe.
In order to solve the above technical problems, the present invention adopts the following technical scheme that:
Alkali extraction and acid precipitation extracts invigorating heart and relaxes the method for flavones ingredient in composition, relaxes the weight of preparation prescription according to invigorating heart Than weighing ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn, by it individually or after mixing, the pH of 6~15 times of amounts is added Value be 8~12 alkaline solution, 90~100 DEG C heating extraction 1~3 hour, filter while hot, filter residue with same method extract 2 times, Merging filtrate；Filtrate pH value is adjusted to 3~5 with acid, is centrifuged or filters after standing, desalination after filter residue redissolves is dry to get flavones Constituents extract.
The method that above-mentioned alkali extraction and acid precipitation extracts flavones ingredient in the easypro composition of invigorating heart, the alkaline solution are alkali soluble Liquid or strong base weak acid salting liquid；Alkali in the aqueous slkali is one of sodium hydroxide, potassium hydroxide, ammonium hydroxide；Described is strong Alkali salt of weak acid is one of sodium carbonate, sodium bicarbonate, potassium carbonate, saleratus；The acid is hydrochloric acid, sulfuric acid, phosphoric acid, vinegar One of acid, tartaric acid, citric acid.
The method that above-mentioned alkali extraction and acid precipitation extracts flavones ingredient in the easypro composition of invigorating heart, weighs people according to recipe quantity The sodium hydroxide that the pH value of 10 times of amounts is 9 is added individually or after mixing in ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn, 95 DEG C heating extraction 1.5 hours, filter while hot, filter residue with same method extract 2 times, merging filtrate；With hydrochloric acid by filtrate pH value 4 are adjusted to, is centrifuged or filters after standing, desalination after filter residue redissolves is dry to get flavones ingredient extract.
The flavones ingredient extract that method described above is extracted.
Alkali extraction and acid precipitation extract invigorating heart relax composition in the method for saponins and ter penoids according to recipe quantity weigh ginseng, The alkalinity that the pH value of 6~15 times of amounts is 8~12 is added individually or after mixing in Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn Solution, 90~100 DEG C heating extraction 1~3 hour, filter while hot, filter residue with same method extract 2 times, merging filtrate；It will with acid Filtrate pH value is adjusted to 6~7, is centrifuged or filters after standing, and desalination after sediment redissolves is dry to get saponins and ter penoids Extract.
Above-mentioned alkali extraction and acid precipitation extracts the method for saponins and ter penoids in the easypro composition of invigorating heart, the alkaline solution For aqueous slkali or strong base weak acid salting liquid；Alkali in the aqueous slkali is one of sodium hydroxide, potassium hydroxide, ammonium hydroxide；Institute The strong base-weak acid salt stated is one of sodium carbonate, sodium bicarbonate, potassium carbonate, saleratus；The acid is hydrochloric acid, sulfuric acid, phosphorus One of acid, acetic acid, tartaric acid, citric acid.
Above-mentioned alkali extraction and acid precipitation extracts the method for saponins and ter penoids in the easypro composition of invigorating heart, claims according to recipe quantity It takes ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn individually or after mixing, the hydrogen-oxygen that the pH value of 10 times of amounts is 9 is added Change sodium, 95 DEG C heating extraction 1.5 hours, filter while hot, filter residue with same method extract 2 times, merging filtrate；With hydrochloric acid by filtrate PH value is adjusted to 6, is centrifuged or filters after standing, and desalination after sediment redissolves is dry to get saponins and ter penoids extract.
The saponins and ter penoids extract that above-mentioned method is extracted.
Above-mentioned effective component includes flavones ingredient extract as claimed in claim 4 and/or according to any one of claims 8 Saponins and ter penoids extract.
The easypro drug of the invigorating heart can be the flavonoids for extracting preceding method and/or saponins and/or terpene After effective component mixes other effective components, be added corresponding auxiliary material made of various pharmaceutical preparations, such as capsule, pill, Syrup, tablet, granule, masticatory etc..
For the drug effect for ensuring invention formulation, applicant has carried out a series of experimental study and sieve to extracting factor Choosing, and comparative experimental study has been carried out to its drug effect.
One, Study on Preparation
1, laboratory apparatus (being shown in Table 1)
Table 1
Instrument title Manufacturer
Rocker300a Vacuum filtration device Xiamen Bi Xi Instrument Ltd.
116 swing pulverizers Hainan Jia Hui food machinery factory
R-220 Rotary Evaporators Its company of Switzerland's cloth
Vacuum oven The permanent laboratory apparatus head factory in Shanghai one
2, flavones ingredient extracting factor screens
In alkali extraction and acid precipitation, solid-to-liquid ratio, alkali extractant, alkaline solution pH value, Extracting temperature, sour precipitating reagent and the heavy pH of acid Value is important factor in order, therefore, to find the preferable extracting factor of flavones ingredient, chooses different solid-to-liquid ratios, alkali Extractant, alkaline solution pH value, Extracting temperature, sour precipitating reagent and the heavy pH value of acid are as investigation factor, according to formula: recovery rate (%)=(total amount of flavones in flavones total amount/recipe quantity in extract) × 100%, calculates the recovery rate of flavones ingredient, knot Fruit is shown in Table 2.
Show according to the literature with correlative study: astragalus flavonid and saponin(e are extracted and separated from Radix Astragali, as the heavy pH of acid When value is 2~3, flavones and the like can be precipitated；And flavones ingredient is precipitated in other Chinese medicines and is mostly used pH value 3~5.The application People passes through further experiment investigation, if the pH value of discovery acid precipitating flavones ingredient is lower than 2, can destroy mother nucleus structure, therefore select Selecting the heavy pH value of acid is 3~5.
Table 2
As seen from the above table, flavones ingredient extraction conditions are as follows: solid-to-liquid ratio is that 1:6~15, the various alkalinity enumerated in table are molten The heavy pH value of various sour precipitating reagents, acid that liquid, alkaline solution pH value are 8~12, Extracting temperature is 90~100 DEG C, are enumerated in table is 3 Better effects, optimal extract process can be reached when~5 are as follows: solid-to-liquid ratio 1:10, alkali extractant are sodium hydroxide, alkali Property solution ph be 9, Extracting temperature is 95 DEG C, and sour precipitating reagent is hydrochloric acid, and the heavy pH value of acid is 4.Wherein, extracting method can be a variety of It such as flows back, decoct；When alkali extractant is the volatile substances such as ammonium hydroxide, only with refluxing extraction.
3, saponins and ter penoids extracting factor screening saponin(e can be divided into Triterpene saponins and steroid saponin, triterpene Saponins are acid saponin(es, and steroid saponin is neutral saponin(e.Astragaloside category Triterpene saponins are neutral slant acidity saponin(es, PH is between 6~7.Again because pH value is lower than 5.0, acid saponin(e is easily destroyed and loses, therefore selecting the heavy pH value of acid is 6~7.
Ter penoids in Schisandra chinensis are mainly triterpene compound, and triterpene compound is that the polycyclic of neutral meta-acid has Machine compound, pH is between 6~7, therefore selecting the heavy pH value of acid is 6~7.
In alkali extraction and acid precipitation, solid-to-liquid ratio, alkali extractant, alkaline solution pH value, Extracting temperature, sour precipitating reagent and the heavy pH of acid Value is important factor in order, therefore, to find the preferable extracting factor of flavones ingredient, chooses different solid-to-liquid ratios, alkali Extractant, alkaline solution pH value, sour precipitating reagent and the heavy pH value of acid are as investigation factor, according to formula: recovery rate (%)=(extract The total amount of saponin(e and terpene in saponin(e and terpene total amount/recipe quantity in object) × 100%, calculate mentioning for saponins and ter penoids Rate is taken, the results are shown in Table 3.
Table 3
As seen from the above table, saponins and ter penoids extraction conditions are as follows: solid-to-liquid ratio be 1:6~15, enumerate in table it is various Various sour precipitating reagents, the acid that alkaline solution, alkaline solution pH value are 8~12, Extracting temperature is 90~100 DEG C, are enumerated in table are heavy PH value can reach better effects, optimal extract process when being 6~7 are as follows: solid-to-liquid ratio 1:10, alkali extractant are hydrogen-oxygen Change sodium, buck pH value is 9, and Extracting temperature is 95 DEG C, and sour precipitating reagent is hydrochloric acid, and the heavy pH value of acid is 6.Wherein, extracting method can be It is a variety of such as to flow back, decoct；When alkali extractant is the volatile substances such as ammonium hydroxide, only with refluxing extraction.
Compared with prior art, the flavones obtained using the extracting method of effective component in the easypro composition of invigorating heart of the invention The recovery rate of class, saponins and ter penoids extract is high, and technique, equipment are simple, easy to operate, non-environmental-pollution, Without using organic solvent, production cost is low, is suitable for industrialized production；And use benefit made from above-mentioned effective component extracts The heart relaxes drug, and effective ingredient content is high, and product safety can reduce preparation dose, and therapeutic effect is more preferable.
Specific embodiment
The embodiment of the present invention 1: a kind of method that alkali extraction and acid precipitation extracts flavones ingredient in the easypro composition of invigorating heart, according to place Side's amount weighs ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn individually or after mixing, and the pH value that 10 times of amounts are added is 9 Sodium hydroxide solution, 95 DEG C decoct 1.5 hours, filter while hot, filter residue with same method decoct 2 times, merging filtrate；Use hydrochloric acid Filtrate pH value is adjusted to 4, is centrifuged or filters after standing, filter residue is dialysed after redissolving, dry to get flavones ingredient extract.
The embodiment of the present invention 2: a kind of method that alkali extraction and acid precipitation extracts flavones ingredient in the easypro composition of invigorating heart, according to place Side's amount weighs ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn individually or after mixing, and the pH value that 6 times of amounts are added is 8 Sodium bicarbonate solution, 90 DEG C decoct 1 hour, filter while hot, and filter residue is decocted 2 times with same method, merging filtrate；It will be filtered with sulfuric acid Liquid pH value is adjusted to 3, is centrifuged or filters after standing, and filter residue is dialysed after redissolving, dry to get flavones ingredient extract.
The embodiment of the present invention 3: a kind of method that alkali extraction and acid precipitation extracts flavones ingredient in the easypro composition of invigorating heart, according to place Side's amount weighs ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn individually or after mixing, and the pH value that 15 times of amounts are added is 12 Potassium hydroxide solution, 100 DEG C decoct 3 hours, filter while hot, filter residue with same method decoct 2 times, merging filtrate；Use phosphoric acid Filtrate pH value is adjusted to 5, is centrifuged or filters after standing, ultrafiltration after filter residue redissolves is dry to get flavones ingredient extract.
The embodiment of the present invention 4: a kind of alkali extraction and acid precipitation extracts invigorating heart and relaxes the method for saponins and ter penoids in composition: Ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn are weighed individually or after mixing according to recipe quantity, and the pH of 10 times of amounts is added The ammonia spirit that value is 9,95 DEG C are flowed back 1.5 hours, are filtered while hot, and filter residue is flowed back 2 times with same method, merging filtrate；Use salt Filtrate pH value is adjusted to 6 by acid, is centrifuged or is filtered after standing, and ultrafiltration after sediment redissolves is dry to get saponins and ter penoids Extract.
The embodiment of the present invention 5: a kind of alkali extraction and acid precipitation extracts invigorating heart and relaxes the method for saponins and ter penoids in composition: Ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn are weighed individually or after mixing according to recipe quantity, and the pH of 6 times of amounts is added The sodium carbonate liquor that value is 8,90 DEG C decoct 1 hour, filter while hot, and filter residue is decocted 2 times with same method, merging filtrate；Use wine Filtrate pH value is adjusted to 6.5 by stone acid, is centrifuged or is filtered after standing, and sediment is dialysed after redissolving, dry to get saponins and terpene Component extract.
The embodiment of the present invention 6: a kind of alkali extraction and acid precipitation extracts invigorating heart and relaxes the method for saponins and ter penoids in composition: Ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn are weighed individually or after mixing according to recipe quantity, and the pH of 15 times of amounts is added The solution of potassium carbonate that value is 12,100 DEG C decoct 3 hours, filter while hot, and filter residue is decocted 2 times with same method, merging filtrate；With Filtrate pH value is adjusted to 7 by citric acid, is centrifuged or is filtered after standing, and sediment is dialysed after redissolving, dry to get saponins and terpene Component extract.
The embodiment of the present invention 7: a kind of alkali extraction and acid precipitation extracts invigorating heart and relaxes the method for saponins and ter penoids in composition: Ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn are weighed individually or after mixing according to recipe quantity, and the pH of 10 times of amounts is added The potassium bicarbonate solution that value is 9,98 DEG C are flowed back 2 hours, are filtered while hot, and filter residue is flowed back 2 times with same method, merging filtrate；With Filtrate pH value is adjusted to 6.5 by acetic acid, is centrifuged or is filtered after standing, and ultrafiltration after sediment redissolves is dry to get saponins and terpene Component extract.
The embodiment of the present invention 8: a kind of invigorating heart relieving capsule is prepared by the following method: any in Example 1~3 The flavones ingredient extract of item, the ter penoids and method D for separately taking the saponin component extracted using method E, method C to extract The polysaccharide component of extraction；The dried object of above-mentioned each effective component is mixed, medicinal powder is ground into；In medicinal powder be added disintegrating agent, Wetting agent, glidant and absorbent are prepared into invigorating heart relieving capsule.
The embodiment of the present invention 9: a kind of easypro glue dripping pill of invigorating heart is prepared by the following method: appointing in Example 4~7 One saponins and ter penoids extract separately takes the polysaccharide component extracted using method D；By above-mentioned each effective component Dried object mixing, is ground into medicinal powder；The medicinal powder is uniformly mixed at 60~80 DEG C with matrix, with 10~50 drops/minute Speed instill in 0~20 DEG C of coolant and relax glue dripping pill to get invigorating heart.
The embodiment of the present invention 10: a kind of easypro glue dispersible tablet of invigorating heart is prepared by the following method: in Example 1~3 The flavones ingredient extract of any one, the saponins and ter penoids extract of any one of Example 4~7, separately takes and adopts The polysaccharide component extracted with method A；The dried object of above-mentioned each effective component is mixed, medicinal powder is ground into；It is added in medicinal powder Disintegrating agent, wetting agent, glidant and absorbent are prepared into the easypro glue dispersible tablet of invigorating heart.
Method A described in above-described embodiment 8-10, method C, method D, method E are the same as the method A in following experimental examples, side Method C, method D, method E.

Claims (9)

  1. It relaxes the method for flavones ingredient in composition 1. alkali extraction and acid precipitation extracts invigorating heart, it is characterised in that: relax preparation according to invigorating heart The weight ratio of prescription weighs ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn, by it individually or after mixing, addition 6~ 15 times amount pH value be 8~12 alkaline solution, 90~100 DEG C heating extraction 1~3 hour, filter while hot, the same sample prescription of filter residue Method is extracted 2 times, merging filtrate；Filtrate pH value is adjusted to 3~5 with acid, is centrifuged or filters after standing, desalination after filter residue redissolves is done It is dry to get flavones ingredient extract.
  2. 2. the method that alkali extraction and acid precipitation according to claim 1 extracts flavones ingredient in the easypro composition of invigorating heart, feature Be: the alkaline solution is aqueous slkali or strong base weak acid salting liquid；Alkali in the aqueous slkali is sodium hydroxide, hydroxide One of potassium, ammonium hydroxide；The strong base-weak acid salt is one of sodium carbonate, sodium bicarbonate, potassium carbonate, saleratus；Institute Stating acid is one of hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, tartaric acid, citric acid.
  3. 3. the method that alkali extraction and acid precipitation according to claim 1 or 2 extracts flavones ingredient in the easypro composition of invigorating heart, special Sign is: weighing ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn individually or after mixing according to recipe quantity, is added 10 Times amount pH value be 9 sodium hydroxide, 95 DEG C heating extraction 1.5 hours, filter while hot, filter residue with same method extract 2 times, close And filtrate；Filtrate pH value is adjusted to 4 with hydrochloric acid, is centrifuged or filters after standing, desalination after filter residue redissolves is dry to get flavonoids Component extract.
  4. 4. the flavones ingredient extract that claims 1 or 2 or 3 the methods are extracted.
  5. 5. alkali extraction and acid precipitation extracts the method for saponins and ter penoids in the easypro composition of invigorating heart, it is characterised in that: according to prescription Amount weighs ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn individually or after mixing, and the pH value that 6~15 times of amounts are added is 8 ~12 alkaline solution, 90~100 DEG C heating extraction 1~3 hour, filter while hot, filter residue with same method extract 2 times, merge Filtrate；Filtrate pH value is adjusted to 6~7 with acid, is centrifuged or filters after standing, desalination after sediment redissolves is dry to get saponins And ter penoids extract.
  6. 6. alkali extraction and acid precipitation according to claim 5 extracts the method for saponins and ter penoids in the easypro composition of invigorating heart, It is characterized by: the alkaline solution is aqueous slkali or strong base weak acid salting liquid；Alkali in the aqueous slkali is sodium hydroxide, hydrogen One of potassium oxide, ammonium hydroxide；The strong base-weak acid salt is sodium carbonate, sodium bicarbonate, potassium carbonate, one in saleratus Kind；The acid is one of hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, tartaric acid, citric acid.
  7. 7. alkali extraction and acid precipitation according to claim 5 or 6 extracts the side of saponins and ter penoids in the easypro composition of invigorating heart Method, it is characterised in that: ginseng, Radix Ophiopogonis, Schisandra chinensis, Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, hawthorn are weighed individually or after mixing according to recipe quantity, Be added 10 times amount pH value be 9 sodium hydroxide, 95 DEG C heating extraction 1.5 hours, filter while hot, filter residue is extracted with same method 2 times, merging filtrate；Filtrate pH value is adjusted to 6 with hydrochloric acid, is centrifuged or filters after standing, desalination after sediment redissolves is dry, i.e., Obtain saponins and ter penoids extract.
  8. 8. saponins and ter penoids extract that claim 5 or 6 or 7 the methods are extracted.
  9. The drug 9. a kind of invigorating heart is relaxed, it is characterised in that: the effective component of the drug include flavonoids as claimed in claim 4 at Divide extract and/or saponins according to any one of claims 8 and ter penoids extract.
CN201811297756.1A 2018-11-01 2018-11-01 Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart Pending CN109288957A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811297756.1A CN109288957A (en) 2018-11-01 2018-11-01 Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811297756.1A CN109288957A (en) 2018-11-01 2018-11-01 Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart

Publications (1)

Publication Number Publication Date
CN109288957A true CN109288957A (en) 2019-02-01

Family

ID=65146243

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811297756.1A Pending CN109288957A (en) 2018-11-01 2018-11-01 Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart

Country Status (1)

Country Link
CN (1) CN109288957A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112451613A (en) * 2020-12-16 2021-03-09 山东罗欣乐康制药有限公司 Preparation method of traditional Chinese medicine oral liquid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112451613A (en) * 2020-12-16 2021-03-09 山东罗欣乐康制药有限公司 Preparation method of traditional Chinese medicine oral liquid
CN112451613B (en) * 2020-12-16 2022-07-05 山东罗欣乐康制药有限公司 Preparation method of traditional Chinese medicine oral liquid

Similar Documents

Publication Publication Date Title
CN101062165B (en) Citrus aurantium total flavone extract and the preparing method thereof
CN101062077B (en) Method for preparing stevia whole stevioside and stevia whole flavone at the same time
CN112972547B (en) Traditional Chinese medicine composition for treating qi and blood deficiency syndrome and preparation method and application thereof
CN102233119B (en) Traditional Chinese medicine composition for treating fatty liver and preparation method thereof
RU2485968C2 (en) Drug preparation for treating diabetes and method for preparing it
CN108783155A (en) A kind of sealwort, mulberry leaf, radix polygonati officinalis compatibility beverage and preparation method thereof
CN103704414B (en) Herbal tea and preparation method thereof
CN106387563A (en) Heat-clearing lung-moistening beverage and preparation method thereof
CN106620610A (en) Preparation method of liquorice heart fire purging granule
CN106377535A (en) A ginsenoside composition, a preparing method thereof and applications of the ginsenoside composition
CN109288957A (en) Alkali extraction and acid precipitation extracts the method and products thereof of effective component in the easypro composition of invigorating heart
CN102716255A (en) Eucommia ulmoides medicinal wine and preparation method thereof
CN105920394A (en) Fruit-flavored lung clearing and phlegm eliminating gel for children and preparation method of gel
CN106928376B (en) The separation method of skunk bush polysaccharide and its application
CN104435658B (en) A kind of medicine for ischemia apoplexy prevention and treatment and preparation method thereof
CN105533737B (en) A kind of black garlic oral solution and its preparation method and application containing Folium Artemisiae Argyi extract
CN114431470A (en) Astragalus stem and leaf decoction and preparation method thereof
CN112741237A (en) Concentrated fruit juice capable of keeping stable content of astragaloside and preparation method thereof
CN105664102A (en) Traditional Chinese medicine composition for improving immunity
CN111407836A (en) A compound wine with blood pressure lowering, blood lipid reducing, and blood sugar lowering effects, and its preparation method
CN107137583B (en) Compound Chinese medicine preparation for treating anemia caused by yin deficiency and blood deficiency and its preparing process
CN109394806B (en) Processing method of ginseng and polygonatum odoratum
CN102258571B (en) Formula of children' s antipyretic granule by using radix bupleuri and cassia twig, and its preparation method
CN104920723A (en) Tea drink for joint treatment of diabetes and making method thereof
CN106177416B (en) A kind of traditional Chinese medicinal composition with effect of reducing blood sugar and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20190201

WD01 Invention patent application deemed withdrawn after publication