CN109287417A - A kind of method for potted of potato zinc-deficiency - Google Patents

A kind of method for potted of potato zinc-deficiency Download PDF

Info

Publication number
CN109287417A
CN109287417A CN201810843704.3A CN201810843704A CN109287417A CN 109287417 A CN109287417 A CN 109287417A CN 201810843704 A CN201810843704 A CN 201810843704A CN 109287417 A CN109287417 A CN 109287417A
Authority
CN
China
Prior art keywords
potato
zinc
deficiency
potted
deionized water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810843704.3A
Other languages
Chinese (zh)
Inventor
索海翠
李小波
刘晓津
罗焕明
朱金华
王丽
安康
潘三文
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CROP Research Institute of Guangdong Academy of Agricultural Sciences
Original Assignee
CROP Research Institute of Guangdong Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CROP Research Institute of Guangdong Academy of Agricultural Sciences filed Critical CROP Research Institute of Guangdong Academy of Agricultural Sciences
Priority to CN201810843704.3A priority Critical patent/CN109287417A/en
Publication of CN109287417A publication Critical patent/CN109287417A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06Coating or dressing seed

Abstract

The invention discloses a kind of method for potted of potato zinc-deficiency, the following steps are included: stripping and slicing mixes medicine, takes bottom of which has holes and is lined with the flowerpot of ventilating interlayer, the quartz sand that specification is 1-2mm and specification is 0.4-0.7mm is mixed into dress basin by the proportion of 2-5:1, potato wedge kind is entered in basin, sand 1.5-2.5cm is covered, deionized water is poured;Deionized water is poured in timing, when growing two panels compound leaf and being fully deployed, is pushed root quartz sand aside, is removed female potato, is flattened;Culture in lack of nutritional elements: being poured using the Huo Gelan liquid medium without zinc, periodically pours deionized water, and record growing state extremely harvests.The method for potted, by reducing element source, so that potato shows obvious nutritional deficiency symptom in cultivation cycle.

Description

A kind of method for potted of potato zinc-deficiency
Technical field
The present invention relates to a kind of plant cultivation method more particularly to a kind of method for potted of potato zinc-deficiency.
Background technique
Potato (scientific name: Solanum tuberosum L.) belongs to Solanaceae herbaceous plant, is that the whole world is only second to rice, jade The fourth-largest important cereal crops of rice, wheat, it is closely bound up with the life of the people.
Microelement plays an important role the growth and development of potato, and the function and missing symptom of microelement are always It is the emphasis of scientific research.It is many to be easy to get by the crop nutritional deficiency symptom of seminal propagation, because seed is small in size, micro member Cellulose content is low, nutritional deficiency symptom easily occurs.But potato is usually not easy to obtain true seed, and seed germination rate is not high, main at present It to be bred in a manner of stem tuber, and stem tuber micronutrient levels is higher, in the course of cultivation, the microelement that stem tuber contains Plant strain growth and yield composition can be substantially met, therefore even if not outer apply microelement, it is also difficult to obtain typical potato Nutritional deficiency symptom.
Forefathers (Li Jun etc.: using potato as the trial of plant physiology Comprehensive Experiment material, laboratory science, 2013,2 (16): 16-18 potato young shoot and potato tissue culture detoxic seedling) is utilized, it is observed using culture solution of element deficient culture for material Seedling Stage nutritional deficiency symptom.As a result, it has been found that tissue-cultured seedling is most of dead after transplanting seedlings, effect is bad;It is transplanting seedlings for material using young shoot High survival rate, and it is observed that nutritional deficiency symptom, but culture vessel is vial, and potato knot potato needs dark surrounds and sky Between, therefore this cultural method can only be limited to seedling stage, can not carry out nutritional deficiency to potato entire growth period, especially to the shadow of yield Loud research, therefore there is certain limitation.
Summary of the invention
For overcome the deficiencies in the prior art, potato zinc-deficiency disease can effectively be protruded the purpose of the present invention is to provide a kind of The method for potted of the potato zinc-deficiency of shape.
The purpose of the present invention adopts the following technical scheme that realization:
A kind of method for potted of potato zinc-deficiency, comprising the following steps:
Medicine is mixed in stripping and slicing: potato seed is cut into potato wedge, mixes medicinal powder by cutter disinfection: the medicinal powder is containing thiophanate methyl, strepto- The talcum pulvis of element;
Plantation: it takes bottom of which has holes and is lined with the flowerpot of ventilating interlayer, by specification be 1-2mm and specification is 0.4-0.7mm's Quartz sand mixes dress basin by the proportion of 2-5:1, and potato wedge kind is entered in basin, sand 1.5-2.5cm is covered, pours deionized water;
It removes female potato: periodically pouring deionized water, when growing two panels compound leaf and being fully deployed, push root quartz sand aside, go mother Potato, leveling;
Culture in lack of nutritional elements: being poured using the Huo Gelan liquid medium without zinc, periodically pours deionized water, records growing state To harvest.
Further, stripping and slicing is mixed in medicine step, is cut into the potato wedge of weight 20-30g, and each potato wedge contains 1-2 bud eye.
Further, stripping and slicing is mixed in medicine step, 0.5% potassium permanganate of cutter or 75% alcohol disinfecting.
Further, in the medicinal powder, the concentration of thiophanate methyl is 3-4wt%, and the concentration of streptomysin is 0.25- 0.35wt%;Every 1kg potato wedge is mixed thoroughly with 0.02kg medicinal powder.
Further, in plantation step, ventilating interlayer is cotton.Cotton not only can effectively stop tiny quartz sand quilt Water is gone out, moreover it is possible to make to provide loose condition and export extra deionized water, effectively avoid extra moisture influence potato emergence and Growth of seedling.
Further, in plantation step, the purity of quartz sand is 99.5-99.9%.Further, specification be 1-2mm and The quartz sand that specification is 0.4-0.7mm mixes dress basin by the proportion of 3:1.
Further, it goes in female potato step, irrigates within every 2-3 days a deionized water before budding.
Compared with prior art, the beneficial effects of the present invention are:
The present invention provide it is a kind of can make the nutritional deficiency phenomenon of kind of potato show more apparent potato potting side Method;Potato is cut into sizeable square, and is dressed seed by using medicinal powder, it is necessary to provide its emergence and shoot growth institute Nutrition simultaneously prevent potato wedge infect germ;It is used as culture substrate by the quartz sand mixing of two kinds of sizes of size, to reduce The source of Zn-ef ficiency provides preferable water content of substrate and air permeability simultaneously for the growth of plant in conventional soil;Both sizes Quartz sand water holding capacity it is fine, the hole provided is advantageous to the development of root system;Ventilating interlayer cotton is padded in bottom, in favor of row Excessive moisture and nutrient solution out grow shoot under suitable humidity, prevent and treat the excessive influence root system respiration of humidity.
The present invention by selection suitably go female potato time point so that shoot can preferably survive, again can be utmostly Ground shows the symptom of nutritional deficiency;The water that present invention watering uses is deionized water, is further reduced the source of element.
Detailed description of the invention
Fig. 1 is the growing way comparison diagram of potato after Culture in lack of nutritional elements 20 days, and the left side is the potato that embodiment 1 harvests, the right The potato harvested for comparative example 1.
Fig. 2 is the plant height of the shoot of embodiment 1 and comparative example 1 after Culture in lack of nutritional elements 20 days;
Fig. 3 is the ground fresh weight grams of embodiment 1 and comparative example 1 after Culture in lack of nutritional elements 10,20 days;
Fig. 4 is the potato wedge fresh weight grams of embodiment 1 and comparative example 1 after Culture in lack of nutritional elements 10,20 days.
Specific embodiment
In the following, being described further in conjunction with the drawings and specific embodiments to the present invention, it should be noted that in not phase Under the premise of conflict, new implementation can be formed between various embodiments described below or between each technical characteristic in any combination Example.
The present invention provides a kind of method for potted of potato zinc-deficiency, comprising the following steps:
Medicine is mixed in stripping and slicing: potato seed is cut into potato wedge, mixes fungicide and antibiotic by cutter disinfection:
In this step, the nutrition for being cut into not easily damaged, the female potato in its bud portion of potato wedge is easier to supply to potato bud, preferably to utilize Apical dominance, germination percentage is higher, is conducive to shoot growth;
Plantation: it takes bottom of which has holes and is lined with the flowerpot of ventilating interlayer, by specification be 1-2mm and specification is 0.4-0.7mm's Quartz sand mixes dress basin by the proportion of 2-5:1, and potato wedge kind is entered in basin, sand 1.5-2.5cm is covered, pours deionized water;
The ventilating interlayer quartz sand compound using size, to provide aperture gap suitable cultivation medium, water conservation is ventilative, together When quartz sand can effectively avoid influence of the nutrient to Culture in lack of nutritional elements process in culture substrate;
It removes female potato: periodically pouring deionized water, when growing two panels compound leaf and being fully deployed, push root quartz sand aside, go mother Potato, leveling;
When growing to two panels compound leaf and being fully deployed, shoot has preferable growing way;And female potato is removed too early, shoot easily dies young, Female potato is removed too late, and the nutritional deficiency symptom of shoot is unobvious;
Culture in lack of nutritional elements: being poured using the Huo Gelan liquid medium without zinc, periodically pours deionized water, records growing state To harvest.
In following specific embodiments, in medicinal powder, the concentration of thiophanate methyl is 3-4wt%, and the concentration of streptomysin is 0.25- 0.35wt%.The preparation method of medicinal powder, includes, but are not limited to, the following ways: by 2.5kg70% thiophanate methyl wettable powder With the agricultural streptomycin of 0.2kg 72%, talcum powder is added to complement to 50kg.
In following specific embodiments, the Huo Gelan culture solution of the Huo Gelan liquid medium and nutritional deficiency that use can be with It is obtained by commercially available means, or is formulated using general research technique.
Embodiment 1:
A kind of method for potted of potato zinc-deficiency, comprising the following steps:
Medicine is mixed in stripping and slicing: cutter being sterilized using 0.5% potassium permanganate disinfection solution, is cut into the potato wedge of weight 25g or so, often 1kg potato wedge is mixed thoroughly with 0.02kg medicinal powder;
Plantation: it takes 25cm bottom of which has holes and is lined with the flowerpot of cotton, by specification be 1-2mm and specification is 0.4-0.7mm's Quartz sand mixes dress basin by the proportion of 3:1, and potato wedge kind is entered in basin, covers the quartz sand of 2cm thickness, pouring deionized water to basin bottom has Water flow exudation;
Remove female potato: deionized water to basin bottom was poured every 2-3 days a water flow exudation, two panels compound leaf to be grown and is fully deployed When, it pushes root quartz sand aside, female potato of exposing is carefully removed, then quartz sand arranged smooth;
Culture in lack of nutritional elements: there is water flow exudation at Huo Gelan liquid medium to the basin bottom for pouring zinc-deficiency for every 2-3 days, records growing state To harvest, plant height, fresh weight, leaf morphology are investigated;
Harvest: after Culture in lack of nutritional elements 90 days, harvesting potato, and when harvest digs out the whole strain of potato, is put into clear water In, after the quartz sand for washing away potato wedge, carry out the investigation such as Analysing Root Characters, yield.
Embodiment 2:
A kind of method for potted of potato zinc-deficiency, comprising the following steps:
Medicine is mixed in stripping and slicing: cutter being used to 75% alcohol disinfecting, is cut into the potato wedge of weight 25g or so, every 1kg potato wedge is used 0.02kg medicinal powder is mixed thoroughly;
Plantation: it takes 25cm bottom of which has holes and is lined with the flowerpot of cotton, by specification be 1-2mm and specification is 0.4-0.7mm's Quartz sand mixes dress basin by the proportion of 3:1, and potato wedge kind is entered in basin, covers the quartz sand of 2cm thickness, pouring deionized water to basin bottom has Water flow exudation;
Remove female potato: deionized water to basin bottom was poured every 2-3 days a water flow exudation, two panels compound leaf to be grown and is fully deployed When, it pushes root quartz sand aside, female potato of exposing is carefully removed, then quartz sand arranged smooth;In next 2 weeks, often Pouring within 2-3 days Huo Gelan liquid medium to basin bottom has water flow exudation;
Culture in lack of nutritional elements: there is water flow exudation at Huo Gelan liquid medium to the basin bottom for pouring zinc-deficiency for every 2-3 days, records growing state To harvest, plant height, fresh weight, leaf morphology are investigated;
Harvest: after Culture in lack of nutritional elements 90 days, harvesting potato, and when harvest digs out the whole strain of potato, is put into clear water In, after the quartz sand for washing away potato wedge, carry out the investigation such as Analysing Root Characters, yield.
Comparative example 1:
A kind of method for potted of potato zinc-deficiency, comprising the following steps:
Medicine is mixed in stripping and slicing: cutter is sterilized using 0.5% potassium permanganate disinfection solution, is cut into the potato wedge of weight 25g or so, every 1kg Potato wedge is mixed thoroughly with 0.02kg medicinal powder;
Plantation: taking 25cm bottom of which has holes and be lined with the flowerpot of cotton, the quartz sand that specification is 1mm is mixed dress basin, by potato Block kind enters in basin, covers the quartz sand of 2cm thickness, pouring deionized water to basin bottom has water flow exudation;
Culture in lack of nutritional elements: after growing two panels compound leaf and being fully deployed, the Huo Gelan liquid medium of zinc-deficiency is poured within every 2-3 days extremely There is water flow exudation at basin bottom, and record growing state investigates plant height, fresh weight, leaf morphology to harvesting;
Harvest: while with Examples 1 and 2, harvesting potato, and when harvest digs out the whole strain of potato, is put into In clear water, after the quartz sand for washing away potato wedge, the investigation such as Analysing Root Characters, yield is carried out.
Performance detection and effect assessment
1. growing way compares
After Culture in lack of nutritional elements 20 days, the growing way of the potato of embodiment 1 and comparative example 1 is as shown in Figure 1.Embodiment 2 with it is right The plant height of the shoot of ratio 1 is as shown in Fig. 2, removing female potato the 10th day and the 20th day, the grams ratio of ground fresh weight and potato wedge fresh weight Relatively as shown in Figure 3-4.As shown in Fig. 2-3, after removing female potato, the potato wedge fresh weight of the potato of shoot ratio at the 10th day does not remove potato The half of block weight is less than and this difference becomes apparent from for 20 days after going potato wedge.Thus illustrate, removing female potato can further improve The performance state of nutritional deficiency.
2. zinc content compares
The 10th day of Culture in lack of nutritional elements and the 30th day, the shoot of Example 1 and comparative example 1, was carried out it containing zinc respectively Amount detection, result is as shown in the table, unit: mg.kg-1DW。
As seen from the above table, 10 days of zinc-deficiency culture and the 30th day, the intracorporal zinc of the plant of embodiment 2 and comparative example 1 Content has apparent difference, this difference shows the most obvious in leaf.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto, The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention Claimed range.

Claims (8)

1. a kind of method for potted of potato zinc-deficiency, which comprises the following steps:
Medicine is mixed in stripping and slicing: potato seed is cut into potato wedge, mixes medicinal powder by cutter disinfection: the medicinal powder is containing thiophanate methyl, streptomysin Talcum pulvis;
Plantation: it takes bottom of which has holes and is lined with the flowerpot of ventilating interlayer, by the quartz that specification is 1-2mm and specification is 0.4-0.7mm Sand mixes dress basin by the proportion of 2-5:1, and potato wedge kind is entered in basin, sand 1.5-2.5cm is covered, pours deionized water;
It removes female potato: periodically pouring deionized water, when growing two panels compound leaf and being fully deployed, push root quartz sand aside, remove female potato, it is whole It is flat;
Culture in lack of nutritional elements: being poured using the Huo Gelan liquid medium without zinc, periodically pours deionized water, and record growing state is extremely received It obtains.
2. the method for potted of potato zinc-deficiency as described in claim 1, which is characterized in that stripping and slicing is mixed in medicine step, and weight is cut into The potato wedge of 20-30g, each potato wedge contain 1-2 bud eye.
3. the method for potted of potato zinc-deficiency as described in claim 1, which is characterized in that stripping and slicing is mixed in medicine step, and cutter makes With 0.5% potassium permanganate or 75% alcohol disinfecting.
4. the method for potted of potato zinc-deficiency as claimed in claim 3, which is characterized in that in the medicinal powder, thiophanate methyl Concentration be 3-4wt%, the concentration of streptomysin is 0.25-0.35wt%;Every 1kg potato wedge is mixed thoroughly with 0.02kg medicinal powder.
5. the method for potted of potato zinc-deficiency as described in claim 1, which is characterized in that in plantation step, ventilating interlayer is Cotton.
6. the method for potted of potato zinc-deficiency as described in claim 1, which is characterized in that in plantation step, quartz sand it is pure Degree is 99.5-99.9%.
7. the method for potted of potato zinc-deficiency as described in claim 1, which is characterized in that specification is 1-2mm and specification is The quartz sand of 0.4-0.7mm mixes dress basin by the proportion of 3:1.
8. the method for potted of potato zinc-deficiency as described in claim 1, which is characterized in that it goes in female potato step, it is every before sprouting Irrigate within 2-3 days a deionized water.
CN201810843704.3A 2018-07-27 2018-07-27 A kind of method for potted of potato zinc-deficiency Pending CN109287417A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810843704.3A CN109287417A (en) 2018-07-27 2018-07-27 A kind of method for potted of potato zinc-deficiency

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810843704.3A CN109287417A (en) 2018-07-27 2018-07-27 A kind of method for potted of potato zinc-deficiency

Publications (1)

Publication Number Publication Date
CN109287417A true CN109287417A (en) 2019-02-01

Family

ID=65172666

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810843704.3A Pending CN109287417A (en) 2018-07-27 2018-07-27 A kind of method for potted of potato zinc-deficiency

Country Status (1)

Country Link
CN (1) CN109287417A (en)

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1045906A (en) * 1989-03-11 1990-10-10 南朝鲜科学技术研究院 The method of a large amount of productive manpower seed potatos (potato microtubers)
JP2000158853A (en) * 1998-11-30 2000-06-13 Asahi Beer Yakuhin Kk Latent trace nutrient deficiency disease discovering system and inquiry card set used therefor
WO2004067699A2 (en) * 2003-01-27 2004-08-12 Plant Research International B.V. Compositions comprising lignosulfonates for crop protection and crop improvement
US20060046264A1 (en) * 2004-08-26 2006-03-02 Monsanto Technology Llc Automated seed sampler and methods of sampling, testing and bulking seeds
CN102939890A (en) * 2011-12-07 2013-02-27 河南科技大学 Culture method for plant seedlings
CN103724117A (en) * 2013-12-16 2014-04-16 天津丰润园精细化工科技有限公司 Special slow-release organic fertilizer for potato and a preparation method thereof
CN103787718A (en) * 2013-12-11 2014-05-14 甘肃金宇生态环境工程有限公司 Novel enhanced nutrient green 'LB' functional fertilizer
CN103942555A (en) * 2014-04-11 2014-07-23 海南大学 Method for detecting nitrogen content of plant through images
CN104472336A (en) * 2014-12-05 2015-04-01 四川省农业科学院土壤肥料研究所 Crop nutritional deficiency experimental system
CN205455146U (en) * 2016-03-30 2016-08-17 北京市土肥工作站 Three -dimensional hydroponic device
CN105875231A (en) * 2016-04-12 2016-08-24 江苏徐州甘薯研究中心 Indoor invasiveness-resisting determination method for sweet potato stem nematode
CN205962176U (en) * 2016-05-30 2017-02-22 吉林省农业科学院 Experimental hydroponic device of soybean nutritional deficiency
CN106550748A (en) * 2016-11-04 2017-04-05 覃忠强 A kind of implantation methods of Rhizoma Solani tuber osi

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1045906A (en) * 1989-03-11 1990-10-10 南朝鲜科学技术研究院 The method of a large amount of productive manpower seed potatos (potato microtubers)
JP2000158853A (en) * 1998-11-30 2000-06-13 Asahi Beer Yakuhin Kk Latent trace nutrient deficiency disease discovering system and inquiry card set used therefor
WO2004067699A2 (en) * 2003-01-27 2004-08-12 Plant Research International B.V. Compositions comprising lignosulfonates for crop protection and crop improvement
US20060046264A1 (en) * 2004-08-26 2006-03-02 Monsanto Technology Llc Automated seed sampler and methods of sampling, testing and bulking seeds
CN102939890A (en) * 2011-12-07 2013-02-27 河南科技大学 Culture method for plant seedlings
CN103787718A (en) * 2013-12-11 2014-05-14 甘肃金宇生态环境工程有限公司 Novel enhanced nutrient green 'LB' functional fertilizer
CN103724117A (en) * 2013-12-16 2014-04-16 天津丰润园精细化工科技有限公司 Special slow-release organic fertilizer for potato and a preparation method thereof
CN103942555A (en) * 2014-04-11 2014-07-23 海南大学 Method for detecting nitrogen content of plant through images
CN104472336A (en) * 2014-12-05 2015-04-01 四川省农业科学院土壤肥料研究所 Crop nutritional deficiency experimental system
CN205455146U (en) * 2016-03-30 2016-08-17 北京市土肥工作站 Three -dimensional hydroponic device
CN105875231A (en) * 2016-04-12 2016-08-24 江苏徐州甘薯研究中心 Indoor invasiveness-resisting determination method for sweet potato stem nematode
CN205962176U (en) * 2016-05-30 2017-02-22 吉林省农业科学院 Experimental hydroponic device of soybean nutritional deficiency
CN106550748A (en) * 2016-11-04 2017-04-05 覃忠强 A kind of implantation methods of Rhizoma Solani tuber osi

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
宁运旺等: "氮磷钾缺乏对甘薯前期生长和养分吸收的影响", 《中国农业科学》 *
王玉宏等: "《山区特色种植业》", 30 September 2017, 河北科学技术出版社 *
王跃华等: "钙过量对茶树光合特性及叶绿体超微结构的影响", 《植物营养与肥料学报》 *
辛建华: "钙素对马铃薯生长发育、光合作用及物质代谢影响的研究", 《农业科技辑》 *

Similar Documents

Publication Publication Date Title
CN103651121B (en) A kind of bletilla differentiation, strong seedling culture base
CN103814745A (en) Method for cutting propagation by utilizing lithocarpus polystachyus epicormic branches
CN103478083A (en) Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae
CN104303804B (en) A kind of magnetic treatment promotes the method for tilia miqueliana cuttage root-taking
CN101889547B (en) Aseptic and rapid propagation method of dendrobium devonianum seeds
CN108307911B (en) Root cutting seedling raising method for fish wood
CN103858771B (en) A kind of transplanting method of corn gene plantlet in vitro
CN101983557A (en) In vitro quick breeding method of seedling stem of santal seed embryo
KR20140094042A (en) Mass-production method for seed potatoes
CN109168697B (en) Method for cultivating green-source red plums
CN108887110A (en) The implantation methods of Gynura divaricata
CN104303765B (en) The high-yield planting method of the stem of noble dendrobium
CN107466850B (en) Blueberry plantation and its seedling fast breeding method
CN103609444B (en) Tissue culture method for hemerocallis sempervirens araki
CN106258575B (en) Double-layer substrate for radish lateral branch cuttage and application method
CN104521476A (en) Planting method of strawberry bonsai
CN103430851A (en) Method for improving domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers
CN109287417A (en) A kind of method for potted of potato zinc-deficiency
KR100741816B1 (en) The orchid seed chip and the method of preparing it
JP6530584B2 (en) Method of producing seedlings of licorice genus plant
CN110810249A (en) Culture medium for promoting elongation of sciaenops ocellatus clumps and preparation method and application thereof
CN107509513B (en) Method for cultivating strong pepper seedlings by using barley leaching liquor under continuous cropping condition
CN103931392A (en) Cutting propagation method for ornithogalum caudatum ait bulb
CN107580950B (en) Seedling raising method for Nanchuan Arboria sand bed
CN107197641B (en) Efficient golden camellia hybrid seed sterile sowing and seedling raising method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20190201