CN109174217A - For realizing the micro-fluidic chip and its method of drying process in synthetic reaction - Google Patents
For realizing the micro-fluidic chip and its method of drying process in synthetic reaction Download PDFInfo
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- CN109174217A CN109174217A CN201810892301.8A CN201810892301A CN109174217A CN 109174217 A CN109174217 A CN 109174217A CN 201810892301 A CN201810892301 A CN 201810892301A CN 109174217 A CN109174217 A CN 109174217A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00905—Separation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0678—Facilitating or initiating evaporation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Abstract
The invention discloses a kind of micro-fluidic chips and its method for drying process in organic synthesis, belong to microfluidic chip technology and organic synthesis field.Microchannel is equipped in the micro-fluidic chip, and the both ends of microchannel are respectively equipped with feeder connection and channel outlet；Microchannel is equipped with several concave shaped cavities along journey, and the concave shaped cavity is recessed from vias inner walls to away from channel center side.Drying micro-fluidic chip of the invention, structure is simple, is easy to automate, it can be achieved that the drying desolventizing of fluid and solvent commutation in chip channel, can be widely used for micro-fluidic synthetic reaction.
Description
Technical field
The invention belongs to microfluidic chip technologies and organic synthesis field, and in particular to one kind is for dry in organic synthesis
The micro-fluidic chip and its method of process.
Background technique
Technology of the microflow control technique originating from fluid in a kind of adjusting microfluidic channel, this technology is in Biochemical Engineering
In have a wide range of applications.The appearance of microflow control technique promotes the related fieldss such as organic chemistry, material science and biomedicine
Development, open the new way for obtaining ideal product, at the same on a molecular scale for further investigation mechanism provide possibility.
It is chemically reacted in micro-fluidic chip, improves controllability, safety and the selectivity of reaction, the amount of reagent used
Small, rapid reaction, occupied area are small.Wherein, main two big advantages are: (I) can be mixed in short time/space scale
Conjunction and heat transfer/mass transfer；It is horizontal that (II) can accurately control nm and pL.These advantages be mainly derived from heat and quality transmitting and
Size is related.Fluid volume is small so that Reynolds number is low, fluid increasingly by viscosity rather than inertia is influenced.In addition, in channel
The ratio of surface area and volume is big, it is ensured that the heat transmitting between the thermal uniformity and device and contained fluid of entire reactor
Quickly.
In chemical synthesis, often it is related to dry and desolventizing process, such as synthesis FDG and indirect labelling synthesis
Fluorination reaction during 18F- Octreotide.Need to carry out anhydrous response and exchange of solvent, the device of conventional organic synthesis
Ware realizes drying in which can be convenient, but in microfluidic system, since channel size is too small, and the fluid body of required drying and dehydrating
Product is few, and after entire fluid is full of microchannel, solution can be expelled directly out by boiling heating and ventilation drying from microchannel, so should
Method is difficult to use in micro-fluidic chip, and the micro-fluidic chip synthesis reported at present is mainly realized with the form of PDMS ventilated membrane
Gas-liquid separation, but these applications can not be applied in occasions such as strong acid and strong bases, be limited because using ventilative PDMS material
It is related to the multistep synthesis of anhydrous response.Therefore, the new microflow control technique of one kind is needed to break through this bottleneck, realizes miniflow
The drying and dehydrating and exchange of solvent of fluid in chip channel are controlled, with the multistep synthesis for being related to anhydrous response.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of for realizing the miniflow of drying process in synthetic reaction
Control chip, structure is simple, it is easy to operate, it can be achieved that in chip channel fluid drying and dehydrating and exchange of solvent, can be used for more
Walk synthetic reaction.
Specific technical solution of the present invention is as follows:
It is a kind of that microchannel being equipped in the micro-fluidic chip for realizing the micro-fluidic chip of drying process in synthetic reaction,
And the both ends of microchannel are respectively equipped with feeder connection and channel outlet；Microchannel is equipped with several concave shaped cavities along journey, and described is recessed
Shape chamber is recessed from vias inner walls to away from channel center side.
In the present invention, the thin channel that microchannel refers to for realizing micro-fluidic reaction, size is generally 100-2000 μ
m.Channel center refer to microchannel for the channel centerline position of reagent or gas of circulating, therefore in vias inner walls to
Away from channel center side, recess just refers to that vias inner walls are recessed by channel center towards chip substrates side, but is recessed direction simultaneously
It is not necessarily intended to complete vertical channel inner wall, can be adjusted as needed.
The feeder connection of microchannel can be passed through gas and liquid reagent, and outlet can exclude gas or liquid or gas-liquid mixed
Object.Since concave shaped cavity is opened in vias inner walls, size is also smaller, when liquid reagent flows through the cavity, reagent meeting
Because surface tension is trapped in cavity, without taking microchannel out of by air-flow promotion.Subsequent holding gas is constantly passed through micro-
Channel can be such that intracavitary delay reagent constantly evaporates, and realize drying.The micro-fluidic chip is relative to using PDMS ventilated membrane
Method, can realize reagent drying and dehydrating and exchange of solvent in simple structure, and the occasions such as strong acid and strong base can also
With application.
Preferably, the feeder connection of the microchannel have it is multiple, all feeder connections pass through bypass passage be connected to
The front end of microchannel.Be arranged multiple feeder connections can enable different gas or liquid reactions reagent from different mouths into
Enter microchannel, without using complicated Vavle switching.
Further, there are two the feeder connection of the microchannel preferably has, wherein first passage entrance is as to be dried
The entrance of reagent, entrance of the second channel entrance as dry gas, two feeder connections converge each by a bypass passage
After stream, it is connected to the front end of microchannel.In the present invention, dry gas, which refers to, to be passed through in microchannel, for what reagent was dried
Gas.The set-up mode can conveniently realize the drying and dehydrating to liquid reagent.
Further, the dry gas is inert gas, is occurred to avoid with the effective component in reagent to be dried
Reaction.
Preferably, the microchannel serpentine-like bending distribution in micro-fluidic chip, to increase micro-tunnel length, subtracts
The size of small chip.
Preferably, the horizontally disposed two sides in microchannel of the concave shaped cavity, in order to which reagent passes in and out the concave shaped cavity.
Preferably, the inner surface of the concave shaped cavity towards microchannel is in smooth arc-shaped surface, to avoid dead volume is generated.
Preferably, the micro-fluidic chip uses and the nonreactive corrosion-resistant material of reagent to be dried, preferably stone
English, silicate glass, borate glass.
It is anti-in synthesis using micro-fluidic chip described in any of the above-described scheme that another object of the present invention is to provide a kind of
The middle method for realizing drying process is answered, it is specific as follows: first to inject reagent to be dried by the feeder connection of microchannel micro- logical
Road upstream position；Then dry gas is passed through into microchannel by feeder connection, pushes reagent to be dried under microchannel
Downstream, and it is intracavitary by being constantly stranded in when the concave shaped cavity along journey；It keeps dry gas to be continually fed into, makes the spill
Intracavitary delay reagent constantly evaporates, until dry.
Preferably, the amount of reagent to be dried of injection microchannel is no more than microchannel along effective appearance of Cheng Suoyou concave shaped cavity
Product.The reagent of all injections can be fully retained in microchannel as a result, will not be carried over microchannel.For concave shaped cavity,
Its dischargeable capacity refer to reagent to be dried by dry gas promotion flow through concave shaped cavity when, the intracavitary maximum reagent that can be accommodated
Volume.
Drying micro-fluidic chip of the invention, structure is simple, be easy to automate, it can be achieved that in chip channel fluid it is dry
Dry desolventizing with solvent commutation, can be widely used for micro-fluidic synthetic reaction.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram for realizing the micro-fluidic chip of drying process in synthetic reaction；
Fig. 2 is another structural schematic diagram for realizing the micro-fluidic chip of drying process in synthetic reaction；
Fig. 3 is first use state diagram (reagent injection) of micro-fluidic chip；
Fig. 4 is second use state diagram (dry gas is passed through) of micro-fluidic chip；
Fig. 5 is the third use state diagram (it is gradual drying to be detained reagent) of micro-fluidic chip；
Fig. 6 is the 4th use state diagram (injection of commutation solvent) of micro-fluidic chip；
Appended drawing reference in figure: first passage entrance 1, second channel entrance 2, channel outlet 3, microchannel 4, concave shaped cavity 5, liquid
Body reagent 6, is detained reagent 8 at dry gas 7.
Specific embodiment
The present invention is further elaborated and is illustrated in the following with reference to the drawings and specific embodiments.In each embodiment of the present invention
Technical characteristic under the premise of not conflicting with each other, can carry out the corresponding combination.
Micro-fluidic chip in the present invention can be used for realizing solvent seasoning process or solvent commutation in organic synthesis
Process.Microchannel 4 is equipped in the micro-fluidic chip, and the both ends of microchannel 4 are respectively equipped with feeder connection and channel outlet, are used for
It is passed through and is discharged gas/liquid.Micro-fluidic chip core of the invention is multiple recessed in being arranged along journey path for microchannel 4
Shape chamber 5, concave shaped cavity 5 to away from channel center side from being recessed in vias inner walls.The main function of concave shaped cavity 5 is to dry
It when liquid drier body reagent flows through, can be trapped in cavity under the action of surface tension, when the quantity of concave shaped cavity 5 is enough
When, all reagents can be all trapped in concave shaped cavity 5, so that it will not take microchannel out of by gas.And due to concave shaped cavity 5 with
What microchannel was connected to, therefore subsequent holding gas is constantly passed through microchannel, and intracavitary delay reagent can be made constantly to evaporate.It is recessed
The size and shape of shape chamber 5 are unlimited, open up direction in vias inner walls, i.e., recess direction, which also can according to need, is adjusted
It is whole.The depth of concave shaped cavity 5 will affect the effect of evaporation drying, and increasing depths are conducive to accommodate solution, increase single treatment amount；Subtract
Small depth, it is possible to increase evaporation rate, but single treatment amount can be reduced；Likewise, the openings of sizes of concave shaped cavity 5 can also change evaporation
Area, and then change evaporation rate.One or more can be set in feeder connection and channel outlet, according to the specific requirement of reaction
It is determined.When a feeder connection is only arranged, different liquid reagents and gas need to inject by Vavle switching mode
The feeder connection, when feeder connection have it is multiple when, the gas or liquid reagent that can be injected each entrance by bypass passage are equal
It is passed through the front end of microchannel 4.When there is multichannel branch channel, each bypass passage is connecting microchannel 4 after can converging together, can also
To be respectively connected to the different location of microchannel 4, but should be noted to need to push reagent to be dried due to dry gas, because
This is passed through on-position of the bypass passage of dry gas on microchannel 4, should be located at the branch for being passed through reagent to be dried as far as possible
The upstream of the on-position in channel or same position.
As shown in Figure 1, for the microfluidic chip structure signal in an embodiment for realization drying process in synthetic reaction
Scheme, microchannel 4 is equipped in the micro-fluidic chip.Micro-fluidic chip can be used quartz, silicate glass, borate glass etc. with to
The nonreactive corrosion-resistant material of dried reagent is made.One end of micro-fluidic chip is set there are two feeder connection, wherein first passage
Entrance of the entrance 1 as reagent to be dried, entrance of the second channel entrance 2 as dry gas, two feeder connection distributions are set
Two bifurcated mouth ends of the connecting line of " V " type structure are placed in, two entrances are converged each by a bypass passage
Afterwards, it is connected to the front end of microchannel 4, reagent to be dried and dry gas are passed through microchannel 4 by distribution.The end of microchannel 4 is equipped with
Channel outlet 3, dry gas or gas-liquid mixture will be flowed out from the outlet.The serpentine-like bending in microchannel 4 is distributed in chip,
It is equipped with multiple concave shaped cavities 5 along journey.Concave shaped cavity 5 in the present embodiment is in half elliptic, from channel cavity position to chip substrates
Side recess, long axis direction are vertical with the inner wall of microchannel 4.The inner surface of concave shaped cavity 5 towards microchannel 4 keeps light as far as possible
It is sliding, no dead angle.The main straightway that is continuous, being set to microchannel 4 in pairs of concave shaped cavity 5, the turning section in microchannel 4 can be not provided with.
In addition, on that basi of the above embodiments, concave shaped cavity 5 opens up angle and can be optimized for level and open up, i.e. chip water
Under level state, by the horizontally disposed two sides in microchannel 4 of concave shaped cavity 5, the conduit wall of two sides is recessed to substrate side respectively.Spill
The extreme lower position of chamber 5 is not less than the bottom of microchannel 4 as far as possible.In a preferred embodiment, micro-fluidic chip having a size of 100mm ×
40mm × 2mm, in rectangle with a thickness of 1mm, width 1.8mm, channel effective length is 850mm, is led to for the cross section of microchannel 4
Road dischargeable capacity is 1530 μ L.The bottom surface of concave shaped cavity 5 is horizontal, and keeps concordant with 4 bottom surface of microchannel, and thickness also leads to micro-
Road 4 is consistent, and minimizes dead volume.
The quantity of 4 shape of microchannel, size and concave shaped cavity 5 in micro-fluidic chip can be according to actually being adjusted
It is whole.For example, in another embodiment, micro-fluidic chip can be set to shape and structure as shown in Figure 2, two feeder connection point
Cloth is set to two bifurcated mouth ends of the connecting line of a Y-shaped structure, and microchannel 4 is shorter relative to Fig. 1, along journey cloth
5 quantity of concave shaped cavity set is also less, therefore the reagent that its single can be dried is also less.
Below by taking micro-fluidic chip shown in Fig. 2 as an example, illustrate to realize in synthetic reaction using the micro-fluidic chip dry
The method of dry process.As shown in figure 3, liquid reagent 6 to be dried is injected by the first passage entrance 1 of microchannel 4 first
The equipment such as syringe pump can be used in 4 upstream position of microchannel, injection process, and in order to guarantee that liquid reagent 6 will not remain in branch
In channel, liquid reagent 6 can be advanced into the joint of Y-shaped structure with portion gas again after having injected liquid reagent 6
Downstream.
As shown in figure 4, being then passed through dry gas 7 into microchannel 4 by second channel entrance 2, dry gas should be protected
Card is not reacted with reagent, therefore the inert gases such as nitrogen, argon gas, helium may be selected.Dry gas 7 pushes liquid reagent 6 to continue
To the lower downstream of microchannel 4, and along journey pass through each concave shaped cavity 5 when, be detained full of cavity and under the action of surface tension
In in cavity.Therefore, liquid reagent 6 is along journey flow process, and being detained reagent 8, constantly filling is along the concave shaped cavity 5 of journey, microchannel
Amount of reagent in 4 constantly reduces.In the synthetic reaction for needing accurate quantification, the reagent to be dried quilt of all injections should ensure that
Drying in microchannel 4 is stayed in, and cannot be taken out of pipeline by dry gas, therefore need to guarantee the to be dried of bolus injection microchannel 4
Amount of reagent (by volume), along the dischargeable capacity of Cheng Suoyou concave shaped cavity 5, reduces the substance for needing to retain in liquid phase no more than microchannel 4
Loss, ensure that subsequent synthesis and the reaction raw materials that accurately measure be provided.In general, the dischargeable capacity of all concave shaped cavities 5
More preferably greater than amount of reagent to be dried, to improve reliability.
Certainly, if synthetic reaction does not need accurate quantification, it is only necessary to substantially realize reagent drying, can it is different it is customized should
It is required that partially liq reagent 6 can be carried over channel outlet 3 with gas.
As shown in figure 5, after the liquid reagent 6 in microchannel 4 is completely filled into concave shaped cavity 5 or extra liquid
Body reagent 6 continues that dry gas is kept to be continually fed into, in concave shaped cavity 5 under the promotion of dry gas 7 behind passing away outlet 3
8 surface of delay reagent flow through with dry gas and constantly evaporate, until reagent is completely dried, or reach scheduled dry journey
Degree (suitable for not requiring all reagents to need dry reaction).
As shown in fig. 6, the substance stayed in after drying in each concave shaped cavity 5 can by injecting other solvents to feeder connection,
It is discharged under gas push from channel outlet 3 after being dissolved, and injects other micro-fluidic chips and continue subsequent reactions.Alternatively,
Other reagents can also be injected in microchannel 4, directly be reacted in channel.
Certainly, in actual drying process, if the Facing material of microchannel 4 has certain hydrophily, portion is also had
Divide liquid reagent 6 to spread over channel surface and form liquid film, this part liquid film also can be as the delay reagent in concave shaped cavity 5, quilt
Dry gas 7 is constantly evaporated.If the surface of microchannel 4 does not have hydrophily, liquid reagent substantially can be concentrated in concave shaped cavity
In.But two kinds of situations can be realized drying.
In addition, needing to heat solution reagent in the reaction of part, therefore the bottom of micro-fluidic chip can be set and add
Hot device, heater are preferably realized using microstructures such as heating chips.
In order to make those skilled in the art more fully understand essence of the invention, below based on18In F-FDG18F-Drying
Dehydration and solvent exchange procedure are illustrated the application method of chip of the invention
Application examples 1:
Using chip shown in fig. 1 or fig. 2, it can be achieved that efficiently on-line automatic be combined to18In F-FDG18F-Drying and dehydrating
Process.Specific operating process is as follows: will using precise injection pump18F-Eluent injected from first passage entrance 1 it is snakelike micro-
4 upstream position of channel, eluent enter after chip microchannel 4, and the heter temperature under chip rises to 80 DEG C, pass through second
The helium injection gas into snakelike microchannel 4 of feeder connection 2.Then, it is pumped using precise injection, by anhydrous acetonitrile CH3CN is from first passage
Entrance 1 injects snakelike 4 the inside of microchannel, mixes with eluent.Acetonitrile can form azeotropic mixture with water, thus when reducing water boiling
Temperature, and make solvent without interfering in reaction solution.The azeotropic mixture that acetonitrile and water are formed is under the promotion of helium, constantly
To the lower downstream of microchannel, until the channel outlet 3 of micro-fluidic chip.Azeotropic mixture is constantly filled along journey flow process
And be stranded in along the concave shaped cavity 5 of journey, the amount of reagent in microchannel 4 constantly reduces, until be stranded in concave shaped cavity 5 completely,
Helium can directly run through from second channel entrance 2 and be discharged from channel outlet 3 at this time, keep constantly being passed through for helium, stagnant
The eluent stayed will constantly evaporate, until dry.Then the heater for controlling chip bottom stops heating, to micro-fluidic chip
Temperature is down to room temperature, stops helium injection gas, dry reactant is obtained in concave shaped cavity 5.
Application examples 2:
Using chip shown in fig. 1 or fig. 2, efficiently on-line automatic be combined to can also be achieved18Exchange of solvent mistake in F-FDG
Journey.Specific operating process is as follows: after fluorination reaction, obtaining the FDG/ acetonitrile solution of acetylation.Being pumped using precise injection will
The FDG/ acetonitrile solution of acetylation injects snakelike 4 upstream position of microchannel from first passage entrance 1, and solution enters after chip,
Heter temperature under chip is risen to 100 DEG C, evaporates acetonitrile.Led to by second channel entrance 2 into snakelike microchannel 4
Helium, solution is in the case where the promotion of helium is hit, lower downstream constantly to microchannel, until the channel outlet 3 of micro-fluidic chip.It is molten
Liquid is constantly filled and is stranded in along the concave shaped cavity 5 of journey along journey flow process, and the amount of reagent in microchannel 4 constantly reduces,
Until be stranded in concave shaped cavity 5 completely, He gas can directly run through from second channel entrance 2 and from channel outlet 3 at this time
Discharge keeps constantly being passed through for helium, and acetonitrile steam will constantly evaporate and be carried over channel in the solution of delay, and other at
Divide and then stay in concave shaped cavity, acetonitrile is constantly evaporated up to and evaporates.After removing acetonitrile, heater stops heating, to micro-fluidic
Chip temperature is down to room temperature, stops helium injection gas, obtains mixture.
Above-mentioned two concrete application examples for chip of the present invention, by the chip be installed on it is on-line automatic be combined in system,
By equipment such as automation control pump, valve, heaters, the techniques such as the drying of automation reagent and exchange of solvent can be realized.When
So, it is anti-to can be used for multistep synthesis for the drying and dehydrating and exchange of solvent that other fluids in channel can also be carried out using the chip
It answers.
Therefore above-mentioned embodiment is only a preferred solution of the present invention, so it is not to limit this hair
It is bright.Those of ordinary skill in related technical field can also be made each without departing from the spirit and scope of the present invention
Kind variation and modification.All mode technical solutions obtained for taking equivalent substitution or equivalent transformation, all fall within guarantor of the invention
It protects in range.
Claims (10)
1. a kind of for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that the micro-fluidic chip
In be equipped with microchannel (4), and the both ends of microchannel (4) are respectively equipped with feeder connection and channel outlet；Microchannel (4) is equipped with along journey
Several concave shaped cavities (5), the concave shaped cavity (5) are recessed from vias inner walls to away from channel center side.
2. as described in claim 1 for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that described
The feeder connection of microchannel (4) have it is multiple, all feeder connections pass through bypass passage connection microchannel (4) front end.
3. as claimed in claim 2 for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that described
There are two the feeder connection tools of microchannel (4), wherein entrance of the first passage entrance (1) as reagent to be dried, second channel
Entrance of the entrance (2) as dry gas after two feeder connections converge each by a bypass passage, is connected to microchannel
(4) front end.
4. as claimed in claim 2 for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that described
Dry gas be inert gas.
5. as described in claim 1 for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that described
Microchannel (4) serpentine-like bending distribution in micro-fluidic chip.
6. as described in claim 1 for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that described
The horizontally disposed two sides in microchannel (4) of concave shaped cavity (5).
7. as described in claim 1 for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that described
Concave shaped cavity (5) towards microchannel (4) inner surface be in smooth arc-shaped surface.
8. as described in claim 1 for realizing the micro-fluidic chip of drying process in synthetic reaction, which is characterized in that described
Micro-fluidic chip use and the nonreactive corrosion-resistant material of reagent to be dried, preferably quartz, silicate glass, borate glass
Glass.
9. the side that a kind of micro-fluidic chip using as described in claim 1~8 is any realizes drying process in synthetic reaction
Method, which is characterized in that reagent to be dried is injected into microchannel (4) upstream position by the feeder connection of microchannel (4) first；So
Dry gas is passed through in microchannel (4) by feeder connection afterwards, pushes reagent to be dried to the lower downstream of microchannel (4),
And it is constantly stranded in when passing through the concave shaped cavity (5) along journey intracavitary；It keeps dry gas to be continually fed into, makes the concave shaped cavity
(5) the delay reagent in constantly evaporates, until dry.
10. the method for drying process is realized in synthetic reaction as claimed in claim 9, which is characterized in that injection microchannel
(4) amount of reagent to be dried is no more than microchannel (4) along the dischargeable capacity of Cheng Suoyou concave shaped cavity (5).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109985673A (en) * | 2019-04-01 | 2019-07-09 | 融智生物科技（青岛）有限公司 | Micro-fluidic chip |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040115830A1 (en) * | 2002-09-25 | 2004-06-17 | Igor Touzov | Components for nano-scale Reactor |
| CN101737989A (en) * | 2008-11-06 | 2010-06-16 | 霍夫曼-拉罗奇有限公司 | Method for providing a dried reagent in a microfluidic system and microfluidic system |
| WO2010101926A2 (en) * | 2009-03-02 | 2010-09-10 | The Johns Hopkins University | Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption |
| CN101906378A (en) * | 2010-07-05 | 2010-12-08 | 博奥生物有限公司 | Bubble micro valve and bubble micro valve-based micro-fluidic chip |
| US20120111506A1 (en) * | 2010-11-10 | 2012-05-10 | Commissariat A L'energie Atomique Et Aux Ene Alt | Method for the controlled evaporation of a liquid drop in a microfluidic device |
| WO2015001502A1 (en) * | 2013-07-02 | 2015-01-08 | Florida State University Research Foundation | Evaporative edge lithography of a microarray |
| CN105080627A (en) * | 2015-08-25 | 2015-11-25 | 辽宁中医药大学 | Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip |
| CN204865880U (en) * | 2015-08-25 | 2015-12-16 | 辽宁中医药大学 | A micro -fluidic chip for having more screening of material compatibility drug effect |
| CN106345541A (en) * | 2016-08-08 | 2017-01-25 | 广东药科大学 | Diphasic liquid-liquid extraction and electrophoretic separation integration microfluidic device and method |
| US20170173579A1 (en) * | 2015-12-21 | 2017-06-22 | Funai Electric Co., Ltd. | Method and Apparatus for Metering and Vaporizing a Fluid |
| US9752904B2 (en) * | 2015-05-29 | 2017-09-05 | Stichting Imec Nederland | Arrangement for providing information on fluid flow rate |
| CN108273575A (en) * | 2018-02-26 | 2018-07-13 | 北京华科泰生物技术有限公司 | It is a kind of to be used to diagnose joint-detection micro-fluidic chip of anemic disorders and its preparation method and application |
-
2018
- 2018-08-07 CN CN201810892301.8A patent/CN109174217B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040115830A1 (en) * | 2002-09-25 | 2004-06-17 | Igor Touzov | Components for nano-scale Reactor |
| CN101737989A (en) * | 2008-11-06 | 2010-06-16 | 霍夫曼-拉罗奇有限公司 | Method for providing a dried reagent in a microfluidic system and microfluidic system |
| WO2010101926A2 (en) * | 2009-03-02 | 2010-09-10 | The Johns Hopkins University | Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption |
| CN101906378A (en) * | 2010-07-05 | 2010-12-08 | 博奥生物有限公司 | Bubble micro valve and bubble micro valve-based micro-fluidic chip |
| US20120111506A1 (en) * | 2010-11-10 | 2012-05-10 | Commissariat A L'energie Atomique Et Aux Ene Alt | Method for the controlled evaporation of a liquid drop in a microfluidic device |
| WO2015001502A1 (en) * | 2013-07-02 | 2015-01-08 | Florida State University Research Foundation | Evaporative edge lithography of a microarray |
| US9752904B2 (en) * | 2015-05-29 | 2017-09-05 | Stichting Imec Nederland | Arrangement for providing information on fluid flow rate |
| CN105080627A (en) * | 2015-08-25 | 2015-11-25 | 辽宁中医药大学 | Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip |
| CN204865880U (en) * | 2015-08-25 | 2015-12-16 | 辽宁中医药大学 | A micro -fluidic chip for having more screening of material compatibility drug effect |
| US20170173579A1 (en) * | 2015-12-21 | 2017-06-22 | Funai Electric Co., Ltd. | Method and Apparatus for Metering and Vaporizing a Fluid |
| CN106345541A (en) * | 2016-08-08 | 2017-01-25 | 广东药科大学 | Diphasic liquid-liquid extraction and electrophoretic separation integration microfluidic device and method |
| CN108273575A (en) * | 2018-02-26 | 2018-07-13 | 北京华科泰生物技术有限公司 | It is a kind of to be used to diagnose joint-detection micro-fluidic chip of anemic disorders and its preparation method and application |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109985673A (en) * | 2019-04-01 | 2019-07-09 | 融智生物科技（青岛）有限公司 | Micro-fluidic chip |
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