CN109169438A - A kind of cultural method of leech - Google Patents

A kind of cultural method of leech Download PDF

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Publication number
CN109169438A
CN109169438A CN201811263621.3A CN201811263621A CN109169438A CN 109169438 A CN109169438 A CN 109169438A CN 201811263621 A CN201811263621 A CN 201811263621A CN 109169438 A CN109169438 A CN 109169438A
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CN
China
Prior art keywords
net cage
water
leech
follows
aquaculture pond
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811263621.3A
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Chinese (zh)
Inventor
胡才忠
肖凌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Jianjitang Biomedical Technology Co Ltd
Jiangxi Jianjitang Agricultural Development Co Ltd
Original Assignee
Shanghai Jianjitang Biomedical Technology Co Ltd
Jiangxi Jianjitang Agricultural Development Co Ltd
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Filing date
Publication date
Application filed by Shanghai Jianjitang Biomedical Technology Co Ltd, Jiangxi Jianjitang Agricultural Development Co Ltd filed Critical Shanghai Jianjitang Biomedical Technology Co Ltd
Priority to CN201811263621.3A priority Critical patent/CN109169438A/en
Publication of CN109169438A publication Critical patent/CN109169438A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/40Culture of aquatic animals of annelids, e.g. lugworms or Eunice

Abstract

The invention belongs to the invention belongs to technical field of pharmaceutical biotechnology, and in particular to a kind of cultural method of leech, this method specifically include that (1) aquaculture pond is arranged;(2) net cage is arranged;(3) seedling is launched;(4) feeding and water quality management.Applicant is by research, residue after being extracted under soil using Poria cocos water in aquaculture pond, and by the setting of new net cage, on the basis of waste utilization, improves the pharmacological action of leech, reduce heavy-metal residual in leech.

Description

A kind of cultural method of leech
Technical field
The invention belongs to pharmaceutical technology fields, and in particular to a kind of cultural method of leech.
Background technique
Leech, popular name leech, has been described in Shennong's Herbal, has very high medical value;In inland fresh water Growth and breeding in waters is the special medicinal aquatic animals of Chinese tradition, and Chinese medicine is used as medicine after dried product is brewed, is had in treatment Wind, hypertension, the clear stasis of blood, amenorrhoea, traumatic injury and other effects.In recent years new discovery leech preparation is in prevention and treatment cardiovascular and cerebrovascular disease and anticancer Aspect has special efficacy.It in history based on catching naturally, because pesticide, chemical fertilizer etc. in recent years are abused and industrial or agricultural " three wastes " is right The pollution of environment, wild natural resources fall sharply, and with the deep development of leech medical value, there is a huge market demand potential.
Currently, there are mainly three types of modes for hirudiniculture: (1) rice field extensive style cultivates;(2) cage culture;(3) cement pit is supported It grows.Paddy field aquaculture mode has the following problems: on the one hand, feed conversion rate is low and leech harvesting is extremely difficult;On the other hand, nothing The birth and old age, sickness and death situation of method grasp leech.The pool cultivated dispersion life that can not solve leech of current cage culture and cement is asked The problem of topic, especially how water degradation is adjusted in time in breeding process.
Therefore, a kind of new hirudiniculture method is studied, is of great significance.
Summary of the invention
For these reasons, applicant obtains a kind of new hirudiniculture method, this method is mainly wrapped by studying for many years Include: (1) aquaculture pond is arranged;(2) net cage is arranged;(3) seedling is launched;(4) feeding and water quality management.Applicant passes through research, Residue after being extracted under soil using Poria cocos water in aquaculture pond, and by the setting of new net cage, on the basis of waste utilization, mention The pharmacological action of high leech reduces heavy-metal residual in leech.
What the present invention was achieved through the following technical solutions.
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged;
(2) net cage is arranged;
(3) seedling is launched;
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 30-50cm, after spreading the Poria cocos extraction of thickness 3-5cm Residue, spreads the soil dug out, under natural environment, after placing 8-12 days, adds water to depth of water 40-50cm;
Wherein (2) net cage is arranged are as follows:
Net cage surrounding uses PVC material, and high 60cm-70cm, long 50cm-60cm, wide 30cm-40cm, bottom web is using Buddhist nun Imperial material, mesh 40-60 mesh inlay 3 PVC solid tubes according to net cage length at spacer screen 5-8cm, and diameter is in 3-5cm;
Net cage is placed in aquaculture pond, net cage spacing 4-5m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 130-150 item/kilogram leech, is put into net cage, every 1.5-2 kilograms of net cage placement, while Spiral shell is placed in net cage, every net cage places 1.5-2 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super 35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again Return aquaculture pond;
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little In 0.03mg/L;
When leech individual growth is to 35-50g, harvest.
Leech of the present invention includes: Hirudinidae animal whitmania Whitmcznia pigra Whitman, leech Hirudo Nipponica Whitman's or willow leaf leech W/iifmama acranulata Whitman is dry all.
Residue after Poria cocos extraction of the present invention are as follows: the residue after the extraction of Poria cocos water is purchased from Central China medicine company action Company.
Specific embodiment
Below the technical scheme of the invention is illustrated by a specific example, but the scope of the present invention is not limited thereto.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention Range should not be construed as being limited to the specific forms stated in the embodiments, and protection scope of the present invention is also and in art technology Personnel conceive according to the present invention it is conceivable that equivalent technologies mean.Although embodiment of the invention below is retouched It states, but the invention is not limited to above-mentioned specific embodiments and applications field, following specific embodiments is only to show It is meaning property, guiding, rather than it is restrictive.Those skilled in the art under the enlightenment of this specification and are not taking off In the case where the range protected from the claims in the present invention, a variety of forms can also be made, these belong to the present invention The column of protection.
The following tests of the present invention are on the basis of multiple creative test, with the claimed technical solution of the present invention Based on, the conclusive test of the research staff of summary.
Prepare embodiment
Embodiment 1
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged;
(2) net cage is arranged;
(3) seedling is launched;
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 40cm, the residue after spreading the Poria cocos extraction of thickness 4cm, It spreads the soil dug out, under natural environment, after placing 10 days, adds water to depth of water 45cm;
Wherein (2) net cage is arranged are as follows:
Net cage surrounding use PVC material, high 65cm, long 55cm, wide 35cm, bottom web use nylon material, 50 mesh of mesh, 3 PVC solid tubes are inlayed according to net cage length at spacer screen 6.5cm, diameter is in 4cm;
Net cage is placed in aquaculture pond, net cage spacing 4.5m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 140/kilogram leech, is put into net cage, every net cage places 1.75 kilograms, while in net cage Middle placement spiral shell, every net cage place 1.75 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super 35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again Return aquaculture pond;
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little In 0.03mg/L;
When leech individual growth is to 35-50g, harvest.Dead, low temperature drying is scalded with boiling water.
Leech of the present invention includes: Hirudo japonica, eurysome golden thread leech, dark brown leech.
Leech of the present invention includes: Hirudinidae animal whitmania Whitmcznia pigra Whitman, leech Hirudo Nipponica Whitman's or willow leaf leech W/iifmama acranulata Whitman is dry all.
Embodiment 2
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged;
(2) net cage is arranged;
(3) seedling is launched;
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 50cm, the residue after spreading the Poria cocos extraction of thickness 5cm, It spreads the soil dug out, under natural environment, after placing 12 days, adds water to depth of water 50cm;
Wherein (2) net cage is arranged are as follows:
Net cage surrounding use PVC material, high 70cm, long 60cm, wide 40cm, bottom web use nylon material, 60 mesh of mesh, 3 PVC solid tubes are inlayed according to net cage length at spacer screen 8cm, diameter is in 5cm;
Net cage is placed in aquaculture pond, net cage spacing 5m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 150/kilogram leech, is put into net cage, every net cage places 2 kilograms, while putting in net cage Spiral shell is set, every net cage places 2 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super 35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again Return aquaculture pond;
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little In 0.03mg/L;
When leech individual growth is to 35-50g, harvest.
Embodiment 3
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged;
(2) net cage is arranged;
(3) seedling is launched;
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 35cm, the residue after spreading the Poria cocos extraction of thickness 3cm, It spreads the soil dug out, under natural environment, after placing 9 days, adds water to depth of water 40cm;
Wherein (2) net cage is arranged are as follows:
Net cage surrounding use PVC material, high 60cm, long 50cm, wide 30cm, bottom web use nylon material, 40 mesh of mesh, 3 PVC solid tubes are inlayed according to net cage length at spacer screen 5cm, diameter is in 3cm;
Net cage is placed in aquaculture pond, net cage spacing 4m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 135/kilogram leech, is put into net cage, every net cage places 1.5 kilograms, while in net cage Spiral shell is placed, every net cage places 1.5 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super 35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again Return aquaculture pond;
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little In 0.03mg/L;
When leech individual growth is to 35-50g, harvest.
Test 1
Heavy metal and harmful element detection
According to lead, cadmium, arsenic, mercury, copper measuring method (2321 atomic absorption spectrophotometry of general rule or inductively coupled plasma constitution Spectrometry) measurement, lead, which must not cross 10mg/kg, cadmium and must not cross 1mg/kg, arsenic and must not cross 5mg/kg, mercury, must not cross 1mg/kg.
Test result: it is shown in Table 1.
The cultural method of the present invention of table 1 obtains leech medicinal material content of beary metal
Conclusion (of pressure testing): it is above-mentioned experiments have shown that, using cultural method of the present invention, content of beary metal, which meets pharmacopoeial quality standard, to be wanted It asks.
Test 2
Antithrombin activity
Detection method: [assay] is detected under " Chinese Pharmacopoeia " 2015 editions 83-84 pages of leech.
Testing result: 2 are shown in Table.
The different embodiment antithrombin activities of table 2
Conclusion (of pressure testing): it is above-mentioned experiments have shown that, cultural method of the present invention, which obtains leech medicinal material, has good anticoagulation enzyme activity The effect of property.
Note: the leech of 1-3 of embodiment of the present invention cultivation meets Chinese Pharmacopoeia requirement.
It is proved by above-mentioned test, the cultural methods such as building of residue and net cage, right after the present invention is extracted using Poria cocos It influences, is of crucial importance in the heavy metal and antithrombin activity of leech.

Claims (5)

1. a kind of hirudiniculture method, it is characterised in that: this method are as follows:
(1) aquaculture pond is arranged;
(2) net cage is arranged;
(3) seedling is launched;
(4) feeding and water quality management.
2. a kind of hirudiniculture method according to claim 1, wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 30-50cm, residual after spreading the Poria cocos extraction of thickness 3-5cm Slag, spreads the soil dug out, under natural environment, after placing 8-12 days, adds water to depth of water 40-50cm.
3. a kind of hirudiniculture method according to claim 1, wherein (2) net cage is arranged are as follows:
Net cage surrounding uses PVC material, and high 60cm-70cm, long 50cm-60cm, wide 30cm-40cm, bottom web is using nylon material Material, mesh 40-60 mesh inlay 3 PVC solid tubes according to net cage length at spacer screen 5-8cm, and diameter is in 3-5cm;
Net cage is placed in aquaculture pond, net cage spacing 4-5m.
4. a kind of hirudiniculture method according to claim 1, wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 130-150 item/kilogram leech, is put into net cage, every net cage places 1.5-2 kilograms, while in net cage Middle placement spiral shell, every net cage place 1.5-2 kilograms of spiral shell.
5. a kind of hirudiniculture method according to claim 1, wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is more than 35 DEG C, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put back to again feeding Grow pond;
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is not more than 0.03mg/L;
When leech individual growth is to 35-50g, harvest.
CN201811263621.3A 2018-10-28 2018-10-28 A kind of cultural method of leech Pending CN109169438A (en)

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Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
CN201811263621.3A CN109169438A (en) 2018-10-28 2018-10-28 A kind of cultural method of leech

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CN109169438A true CN109169438A (en) 2019-01-11

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110447583A (en) * 2019-09-18 2019-11-15 克小改 A kind of ecological ideas aquiculture net cage and its application method

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2793566A1 (en) * 2011-12-20 2014-10-29 Arnvid Skogseth System and method for inhibiting parasites to infest and attach to aquatic animals
CN104886006A (en) * 2015-06-19 2015-09-09 广西复鑫益生物科技有限公司平南分公司 Paddy field leech mixed-culture method
CN105191865A (en) * 2014-11-09 2015-12-30 长沙百禾百生物科技有限公司 Leech ecological aquaculture technology
CN106804489A (en) * 2016-12-14 2017-06-09 五河县茂源水蛭生态养殖专业合作社 A kind of cultural method of hirudo provincialis
CN107279010A (en) * 2017-08-17 2017-10-24 钟春明 A kind of plant of cray
CN107935339A (en) * 2017-10-27 2018-04-20 河海大学 A kind of aquaculture pond bed mud heavy metal stabilizer, its production method and its application
CN107980675A (en) * 2017-11-06 2018-05-04 合肥康青源养殖有限公司 Hementaria officianalis high-density breeding method
CN108651704A (en) * 2017-03-31 2018-10-16 李群庆 A kind of ecological circulation kind cultural method

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2793566A1 (en) * 2011-12-20 2014-10-29 Arnvid Skogseth System and method for inhibiting parasites to infest and attach to aquatic animals
CN105191865A (en) * 2014-11-09 2015-12-30 长沙百禾百生物科技有限公司 Leech ecological aquaculture technology
CN104886006A (en) * 2015-06-19 2015-09-09 广西复鑫益生物科技有限公司平南分公司 Paddy field leech mixed-culture method
CN106804489A (en) * 2016-12-14 2017-06-09 五河县茂源水蛭生态养殖专业合作社 A kind of cultural method of hirudo provincialis
CN108651704A (en) * 2017-03-31 2018-10-16 李群庆 A kind of ecological circulation kind cultural method
CN107279010A (en) * 2017-08-17 2017-10-24 钟春明 A kind of plant of cray
CN107935339A (en) * 2017-10-27 2018-04-20 河海大学 A kind of aquaculture pond bed mud heavy metal stabilizer, its production method and its application
CN107980675A (en) * 2017-11-06 2018-05-04 合肥康青源养殖有限公司 Hementaria officianalis high-density breeding method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110447583A (en) * 2019-09-18 2019-11-15 克小改 A kind of ecological ideas aquiculture net cage and its application method
CN110447583B (en) * 2019-09-18 2021-08-31 山东海链海洋科技有限公司 Ecological green aquaculture net cage and use method thereof

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