CN109169438A - A kind of cultural method of leech - Google Patents
A kind of cultural method of leech Download PDFInfo
- Publication number
- CN109169438A CN109169438A CN201811263621.3A CN201811263621A CN109169438A CN 109169438 A CN109169438 A CN 109169438A CN 201811263621 A CN201811263621 A CN 201811263621A CN 109169438 A CN109169438 A CN 109169438A
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- net cage
- water
- leech
- follows
- aquaculture pond
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
Abstract
The invention belongs to the invention belongs to technical field of pharmaceutical biotechnology, and in particular to a kind of cultural method of leech, this method specifically include that (1) aquaculture pond is arranged；(2) net cage is arranged；(3) seedling is launched；(4) feeding and water quality management.Applicant is by research, residue after being extracted under soil using Poria cocos water in aquaculture pond, and by the setting of new net cage, on the basis of waste utilization, improves the pharmacological action of leech, reduce heavy-metal residual in leech.
Description
Technical field
The invention belongs to pharmaceutical technology fields, and in particular to a kind of cultural method of leech.
Background technique
Leech, popular name leech, has been described in Shennong's Herbal, has very high medical value；In inland fresh water
Growth and breeding in waters is the special medicinal aquatic animals of Chinese tradition, and Chinese medicine is used as medicine after dried product is brewed, is had in treatment
Wind, hypertension, the clear stasis of blood, amenorrhoea, traumatic injury and other effects.In recent years new discovery leech preparation is in prevention and treatment cardiovascular and cerebrovascular disease and anticancer
Aspect has special efficacy.It in history based on catching naturally, because pesticide, chemical fertilizer etc. in recent years are abused and industrial or agricultural " three wastes " is right
The pollution of environment, wild natural resources fall sharply, and with the deep development of leech medical value, there is a huge market demand potential.
Currently, there are mainly three types of modes for hirudiniculture: (1) rice field extensive style cultivates；(2) cage culture；(3) cement pit is supported
It grows.Paddy field aquaculture mode has the following problems: on the one hand, feed conversion rate is low and leech harvesting is extremely difficult；On the other hand, nothing
The birth and old age, sickness and death situation of method grasp leech.The pool cultivated dispersion life that can not solve leech of current cage culture and cement is asked
The problem of topic, especially how water degradation is adjusted in time in breeding process.
Therefore, a kind of new hirudiniculture method is studied, is of great significance.
Summary of the invention
For these reasons, applicant obtains a kind of new hirudiniculture method, this method is mainly wrapped by studying for many years
Include: (1) aquaculture pond is arranged；(2) net cage is arranged；(3) seedling is launched；(4) feeding and water quality management.Applicant passes through research,
Residue after being extracted under soil using Poria cocos water in aquaculture pond, and by the setting of new net cage, on the basis of waste utilization, mention
The pharmacological action of high leech reduces heavy-metal residual in leech.
What the present invention was achieved through the following technical solutions.
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged；
(2) net cage is arranged；
(3) seedling is launched；
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 30-50cm, after spreading the Poria cocos extraction of thickness 3-5cm
Residue, spreads the soil dug out, under natural environment, after placing 8-12 days, adds water to depth of water 40-50cm；
Wherein (2) net cage is arranged are as follows:
Net cage surrounding uses PVC material, and high 60cm-70cm, long 50cm-60cm, wide 30cm-40cm, bottom web is using Buddhist nun
Imperial material, mesh 40-60 mesh inlay 3 PVC solid tubes according to net cage length at spacer screen 5-8cm, and diameter is in 3-5cm；
Net cage is placed in aquaculture pond, net cage spacing 4-5m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 130-150 item/kilogram leech, is put into net cage, every 1.5-2 kilograms of net cage placement, while
Spiral shell is placed in net cage, every net cage places 1.5-2 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super
35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again
Return aquaculture pond；
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little
In 0.03mg/L；
When leech individual growth is to 35-50g, harvest.
Leech of the present invention includes: Hirudinidae animal whitmania Whitmcznia pigra Whitman, leech Hirudo
Nipponica Whitman's or willow leaf leech W/iifmama acranulata Whitman is dry all.
Residue after Poria cocos extraction of the present invention are as follows: the residue after the extraction of Poria cocos water is purchased from Central China medicine company action
Company.
Specific embodiment
Below the technical scheme of the invention is illustrated by a specific example, but the scope of the present invention is not limited thereto.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention
Range should not be construed as being limited to the specific forms stated in the embodiments, and protection scope of the present invention is also and in art technology
Personnel conceive according to the present invention it is conceivable that equivalent technologies mean.Although embodiment of the invention below is retouched
It states, but the invention is not limited to above-mentioned specific embodiments and applications field, following specific embodiments is only to show
It is meaning property, guiding, rather than it is restrictive.Those skilled in the art under the enlightenment of this specification and are not taking off
In the case where the range protected from the claims in the present invention, a variety of forms can also be made, these belong to the present invention
The column of protection.
The following tests of the present invention are on the basis of multiple creative test, with the claimed technical solution of the present invention
Based on, the conclusive test of the research staff of summary.
Prepare embodiment
Embodiment 1
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged；
(2) net cage is arranged；
(3) seedling is launched；
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 40cm, the residue after spreading the Poria cocos extraction of thickness 4cm,
It spreads the soil dug out, under natural environment, after placing 10 days, adds water to depth of water 45cm；
Wherein (2) net cage is arranged are as follows:
Net cage surrounding use PVC material, high 65cm, long 55cm, wide 35cm, bottom web use nylon material, 50 mesh of mesh,
3 PVC solid tubes are inlayed according to net cage length at spacer screen 6.5cm, diameter is in 4cm；
Net cage is placed in aquaculture pond, net cage spacing 4.5m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 140/kilogram leech, is put into net cage, every net cage places 1.75 kilograms, while in net cage
Middle placement spiral shell, every net cage place 1.75 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super
35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again
Return aquaculture pond；
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little
In 0.03mg/L；
When leech individual growth is to 35-50g, harvest.Dead, low temperature drying is scalded with boiling water.
Leech of the present invention includes: Hirudo japonica, eurysome golden thread leech, dark brown leech.
Leech of the present invention includes: Hirudinidae animal whitmania Whitmcznia pigra Whitman, leech Hirudo
Nipponica Whitman's or willow leaf leech W/iifmama acranulata Whitman is dry all.
Embodiment 2
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged；
(2) net cage is arranged；
(3) seedling is launched；
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 50cm, the residue after spreading the Poria cocos extraction of thickness 5cm,
It spreads the soil dug out, under natural environment, after placing 12 days, adds water to depth of water 50cm；
Wherein (2) net cage is arranged are as follows:
Net cage surrounding use PVC material, high 70cm, long 60cm, wide 40cm, bottom web use nylon material, 60 mesh of mesh,
3 PVC solid tubes are inlayed according to net cage length at spacer screen 8cm, diameter is in 5cm；
Net cage is placed in aquaculture pond, net cage spacing 5m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 150/kilogram leech, is put into net cage, every net cage places 2 kilograms, while putting in net cage
Spiral shell is set, every net cage places 2 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super
35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again
Return aquaculture pond；
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little
In 0.03mg/L；
When leech individual growth is to 35-50g, harvest.
Embodiment 3
A kind of hirudiniculture method, this method are as follows:
(1) aquaculture pond is arranged；
(2) net cage is arranged；
(3) seedling is launched；
(4) feeding and water quality management.
Wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 35cm, the residue after spreading the Poria cocos extraction of thickness 3cm,
It spreads the soil dug out, under natural environment, after placing 9 days, adds water to depth of water 40cm；
Wherein (2) net cage is arranged are as follows:
Net cage surrounding use PVC material, high 60cm, long 50cm, wide 30cm, bottom web use nylon material, 40 mesh of mesh,
3 PVC solid tubes are inlayed according to net cage length at spacer screen 5cm, diameter is in 3cm；
Net cage is placed in aquaculture pond, net cage spacing 4m.
Wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 135/kilogram leech, is put into net cage, every net cage places 1.5 kilograms, while in net cage
Spiral shell is placed, every net cage places 1.5 kilograms of spiral shell.
Wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is super
35 DEG C are crossed, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put again
Return aquaculture pond；
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is little
In 0.03mg/L；
When leech individual growth is to 35-50g, harvest.
Test 1
Heavy metal and harmful element detection
According to lead, cadmium, arsenic, mercury, copper measuring method (2321 atomic absorption spectrophotometry of general rule or inductively coupled plasma constitution
Spectrometry) measurement, lead, which must not cross 10mg/kg, cadmium and must not cross 1mg/kg, arsenic and must not cross 5mg/kg, mercury, must not cross 1mg/kg.
Test result: it is shown in Table 1.
The cultural method of the present invention of table 1 obtains leech medicinal material content of beary metal
Conclusion (of pressure testing): it is above-mentioned experiments have shown that, using cultural method of the present invention, content of beary metal, which meets pharmacopoeial quality standard, to be wanted
It asks.
Test 2
Antithrombin activity
Detection method: [assay] is detected under " Chinese Pharmacopoeia " 2015 editions 83-84 pages of leech.
Testing result: 2 are shown in Table.
The different embodiment antithrombin activities of table 2
Conclusion (of pressure testing): it is above-mentioned experiments have shown that, cultural method of the present invention, which obtains leech medicinal material, has good anticoagulation enzyme activity
The effect of property.
Note: the leech of 1-3 of embodiment of the present invention cultivation meets Chinese Pharmacopoeia requirement.
It is proved by above-mentioned test, the cultural methods such as building of residue and net cage, right after the present invention is extracted using Poria cocos
It influences, is of crucial importance in the heavy metal and antithrombin activity of leech.
Claims (5)
1. a kind of hirudiniculture method, it is characterised in that: this method are as follows:
(1) aquaculture pond is arranged；
(2) net cage is arranged；
(3) seedling is launched；
(4) feeding and water quality management.
2. a kind of hirudiniculture method according to claim 1, wherein (1) aquaculture pond is arranged are as follows:
Aquaculture pond empties water, and sludge is removed, and dirt bed is dug to 30-50cm, residual after spreading the Poria cocos extraction of thickness 3-5cm
Slag, spreads the soil dug out, under natural environment, after placing 8-12 days, adds water to depth of water 40-50cm.
3. a kind of hirudiniculture method according to claim 1, wherein (2) net cage is arranged are as follows:
Net cage surrounding uses PVC material, and high 60cm-70cm, long 50cm-60cm, wide 30cm-40cm, bottom web is using nylon material
Material, mesh 40-60 mesh inlay 3 PVC solid tubes according to net cage length at spacer screen 5-8cm, and diameter is in 3-5cm；
Net cage is placed in aquaculture pond, net cage spacing 4-5m.
4. a kind of hirudiniculture method according to claim 1, wherein (3) seedling is launched are as follows:
Stalwartness is chosen without wound, 130-150 item/kilogram leech, is put into net cage, every net cage places 1.5-2 kilograms, while in net cage
Middle placement spiral shell, every net cage place 1.5-2 kilograms of spiral shell.
5. a kind of hirudiniculture method according to claim 1, wherein (4) feeding and water quality management are as follows:
After net cage launches spiral shell, water peanut, hydrilla verticillata are launched in water, and kept for 20-30 DEG C of water temperature, when water temperature is more than 35
DEG C, net cage is risen, is put into interior, guarantees 20-30 DEG C of room temperature, when water temperature restores 20-30 DEG C, net cage is put back to again feeding
Grow pond；
Holding water pH value is 7.0-7.5, and concentration of hydrogen sulfide is not more than 0.05mg/L in water body, and nitrite concentration is not more than
0.03mg/L；
When leech individual growth is to 35-50g, harvest.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811263621.3A CN109169438A (en) | 2018-10-28 | 2018-10-28 | A kind of cultural method of leech |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811263621.3A CN109169438A (en) | 2018-10-28 | 2018-10-28 | A kind of cultural method of leech |
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| Publication Number | Publication Date |
|---|---|
| CN109169438A true CN109169438A (en) | 2019-01-11 |
Family
ID=64944151
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| CN201811263621.3A Pending CN109169438A (en) | 2018-10-28 | 2018-10-28 | A kind of cultural method of leech |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447583A (en) * | 2019-09-18 | 2019-11-15 | 克小改 | A kind of ecological ideas aquiculture net cage and its application method |
| CN114557298A (en) * | 2022-02-28 | 2022-05-31 | 重庆多普泰制药股份有限公司 | Opening method for artificially breeding young leeches of Japanese medicina |
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| CN107935339A (en) * | 2017-10-27 | 2018-04-20 | 河海大学 | A kind of aquaculture pond bed mud heavy metal stabilizer, its production method and its application |
| CN107980675A (en) * | 2017-11-06 | 2018-05-04 | 合肥康青源养殖有限公司 | Hementaria officianalis high-density breeding method |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2793566A1 (en) * | 2011-12-20 | 2014-10-29 | Arnvid Skogseth | System and method for inhibiting parasites to infest and attach to aquatic animals |
| CN105191865A (en) * | 2014-11-09 | 2015-12-30 | 长沙百禾百生物科技有限公司 | Leech ecological aquaculture technology |
| CN104886006A (en) * | 2015-06-19 | 2015-09-09 | 广西复鑫益生物科技有限公司平南分公司 | Paddy field leech mixed-culture method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447583A (en) * | 2019-09-18 | 2019-11-15 | 克小改 | A kind of ecological ideas aquiculture net cage and its application method |
| CN110447583B (en) * | 2019-09-18 | 2021-08-31 | 山东海链海洋科技有限公司 | Ecological green aquaculture net cage and use method thereof |
| CN114557298A (en) * | 2022-02-28 | 2022-05-31 | 重庆多普泰制药股份有限公司 | Opening method for artificially breeding young leeches of Japanese medicina |
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