CN109134680B - Extraction and purification method of fucoidin and application thereof - Google Patents
Extraction and purification method of fucoidin and application thereof Download PDFInfo
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- 238000000605 extraction Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000000746 purification Methods 0.000 title claims abstract description 21
- 229920000855 Fucoidan Polymers 0.000 claims abstract description 21
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims description 20
- 239000002994 raw material Substances 0.000 claims description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 239000007864 aqueous solution Substances 0.000 claims description 12
- 239000002738 chelating agent Substances 0.000 claims description 11
- 239000012065 filter cake Substances 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 11
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 9
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 9
- 239000001110 calcium chloride Substances 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- 125000003396 thiol group Chemical group [H]S* 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 7
- 238000000227 grinding Methods 0.000 claims description 5
- 239000002002 slurry Substances 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 4
- 241001261506 Undaria pinnatifida Species 0.000 claims description 4
- ACTRVOBWPAIOHC-UHFFFAOYSA-N succimer Chemical compound OC(=O)C(S)C(S)C(O)=O ACTRVOBWPAIOHC-UHFFFAOYSA-N 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 2
- 235000013376 functional food Nutrition 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 abstract description 5
- 229920001282 polysaccharide Polymers 0.000 abstract description 5
- 239000005017 polysaccharide Substances 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 4
- 239000002904 solvent Substances 0.000 abstract description 2
- 238000002604 ultrasonography Methods 0.000 abstract description 2
- 235000013402 health food Nutrition 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 8
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- 241000199919 Phaeophyceae Species 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 229910001626 barium chloride Inorganic materials 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001875 compounds Chemical group 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 229950006191 gluconic acid Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000874 microwave-assisted extraction Methods 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Sustainable Development (AREA)
- Mycology (AREA)
- Botany (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the technical field of polysaccharide extraction, and particularly relates to an extraction and purification method of fucoidan and application thereof. The method comprises three steps of crude extraction, heavy metal removal and purification. The extracted fucoidin can be processed into health food. According to the technical scheme provided by the invention, the product which is further used for food processing and has high content and no heavy metal residue is obtained through three steps of crude extraction, heavy metal removal and purification in sequence, and the three steps are organically linked, so that the extraction process is greatly simplified, the use amount of water and a solvent is greatly reduced, two times of ultrasound are carried out, the extraction efficiency is facilitated, the heavy metal residue is removed, and the high-quality product is obtained.
Description
Technical Field
The invention belongs to the technical field of polysaccharide extraction, and particularly relates to an extraction and purification method of fucoidan and application thereof.
Background
The fucoidan component is limited to only the slimy part extracted from brown algae, and there are many different fucoidans, such as those extracted from kelp, undaria pinnatifida sporophyll and nemacystus, etc. According to recent studies, many biological activities of fucoidan have been disclosed, such as "anticancer effect", "cholesterol-lowering", "blood pressure-lowering" and "antiviral" effects. In the structure of chemical molecule, fucoidan is a high molecular polysaccharide composed of fucose sulfate, and besides, the sugar chain of fucoidan also includes galactose, mannose, xylose and glyconic acid.
The fucoidan extraction method mainly comprises an acid extraction method, a water extraction method and a salt extraction method, and the newly developed extraction method mainly comprises an ultrasonic wave extraction method, a microwave-assisted extraction method and an enzyme-assisted extraction method. The extraction and separation method of fucoidan is particularly critical because the activity of the fucoidan is related to the molecular weight, monosaccharide components, sulfate content and sulfate ester group composition, and sulfate loss and target compound structure transformation are easily caused in the separation process.
In addition, as the raw materials for extracting the fucoidin are marine products, the fucoidin has strong adsorbability to heavy metal ions due to inevitable pollution of seawater, so that some heavy metal ions can be remained in the extracted fucoidin, and troubles are caused for subsequent use of health-care food or medicines.
Disclosure of Invention
The invention aims to provide a method for extracting and purifying fucoidan and application thereof.
In order to solve the technical problems, the technical scheme of the invention is as follows: the extraction and purification method of fucoidin comprises the following steps:
step one, crude extraction: crushing the raw materials, and then obtaining an immersion liquid of fucoidin by adopting an acid extraction method and/or a hot water extraction method;
step two, removing heavy metals: adding a sulfhydryl chelating agent into the steeping liquor, heating and ultrasonically vibrating, and then precipitating by using absolute ethyl alcohol to obtain a filter cake of fucoidin;
step three, purification: the fucoidin is further purified by absolute ethyl alcohol precipitation, quaternary ammonium salt precipitation or solvent extraction.
Optionally, the raw material is one or more of kelp, undaria pinnatifida sporophyll and nemacystus decipiens.
Optionally, the specific process of the step one is as follows: crushing the dried raw materials by a crusher, and soaking the crushed raw materials in 200mL of acetic acid aqueous solution with the pH value of 4-5 for 2-3 hours; filtering, retaining filtrate, further grinding filter cake into slurry, adding water 8-12 times of the weight of the raw material, heating to 30-40 deg.C, ultrasonic extracting for 30-60min, and filtering; the two filtrates are combined and adjusted to pH 6.5-7.5.
Optionally, the sulfhydryl chelator of step two is selected from 1 to 3g of a sulfhydryl chelator (2, 3-dimercaptopropanol-1-sulfonic acid or 2, 3-dimercaptosuccinic acid).
Optionally, the mercapto-chelating agent is used in an amount of 1-3% by weight of the starting material.
Optionally, the specific process of the second step is as follows: adding a sulfhydryl chelator into the solution obtained in the first step, heating to 40-50 ℃, ultrasonically vibrating for 30-60min, adjusting pH value to 6.5-7.5, adding anhydrous ethanol 8-12 times of the weight of the raw material, standing overnight, and filtering.
Optionally, the specific process of the step three is as follows: and (3) dissolving the filter cake obtained in the second step by using a calcium chloride aqueous solution, adding equal volume of absolute ethyl alcohol to precipitate the fucoidan, filtering, washing for 3 times by using the absolute ethyl alcohol, and drying to obtain an extracted product.
Optionally, the concentration of calcium chloride in the calcium chloride aqueous solution is 5-10% wt.
The invention also provides the application of the fucoidin prepared by the purification and purification method in health-care functional food.
Optionally, the food product is a beverage or a brewable powder.
According to the technical scheme provided by the invention, the product which is further used for food processing and has high content and no heavy metal residue is obtained through three steps of crude extraction, heavy metal removal and purification in sequence, and the three steps are organically linked, so that the extraction process is greatly simplified, the use amount of water and a solvent is greatly reduced, two times of ultrasound are carried out, the extraction efficiency is facilitated, the heavy metal residue is removed, and the high-quality product is obtained.
Detailed Description
For the convenience of understanding, the process for extracting and purifying fucoidan is described in the following with reference to examples, which are intended to illustrate the present invention and not to limit the scope of the present invention.
The raw materials used in the examples are all conventional commercial products except for special instructions, and the processing techniques used are all the techniques common in the art except for special instructions.
Example 1
The extraction and purification method of fucoidin comprises the following steps:
step one, crude extraction: crushing dried 100g of raw material (kelp) by a crusher, and soaking the crushed raw material in 200mL of acetic acid aqueous solution with the pH value of 4 for 3 hours; filtering, retaining filtrate, further grinding filter cake into slurry, adding 1L water, heating to 40 deg.C, ultrasonic extracting for 30min, and filtering; combining the two filtrates, and adjusting the pH value to about 7;
step two, removing heavy metals: adding 1g of sulfhydryl chelator (2, 3-dimercaptopropanol-1-sulfonic acid) into the solution obtained in the first step, heating to 50 ℃, ultrasonically vibrating for 60min, adjusting the pH value to about 7 again, adding 1L of absolute ethyl alcohol, standing overnight, and filtering;
step three, purification: and (3) dissolving the filter cake obtained in the second step by using a 10% wt calcium chloride aqueous solution, adding equal volume of absolute ethyl alcohol to precipitate the fucoidan, filtering, washing for 3 times by using the absolute ethyl alcohol, and drying to obtain an extracted product.
Example 2
The extraction and purification method of fucoidin comprises the following steps:
step one, crude extraction: sun-drying 100g of raw material (Nemacystus decipiens), pulverizing with a pulverizer, and soaking in 200mL of acetic acid aqueous solution with pH of 5 for 3 hours; filtering, retaining filtrate, further grinding filter cake into slurry, adding 1L water, heating to 30 deg.C, ultrasonic extracting for 60min, and filtering; combining the two filtrates, and adjusting the pH value to about 7;
step two, removing heavy metals: adding 3g of sulfhydryl chelator (2, 3-dimercaptosuccinic acid) into the solution obtained in the first step, heating to 40 ℃, ultrasonically vibrating for 30min, adjusting the pH value to about 7 again, adding 1L of absolute ethyl alcohol, standing overnight, and filtering;
step three, purification: and (3) dissolving the filter cake obtained in the second step by using 8% wt of calcium chloride aqueous solution, adding equal volume of absolute ethyl alcohol to precipitate the fucoidan, filtering, washing for 3 times by using the absolute ethyl alcohol, and drying to obtain an extracted product.
Example 3
The extraction and purification method of fucoidin comprises the following steps:
step one, crude extraction: sun-drying 100g of raw materials (sea tangle, Undaria pinnatifida sporophyll and Nemacystus decipiens and mixture), pulverizing with a pulverizer, and soaking in 200mL of acetic acid aqueous solution with pH of 4 for 2 hours; filtering, retaining filtrate, further grinding filter cake into slurry, adding 1L water, heating to 30 deg.C, ultrasonic extracting for 50min, and filtering; combining the two filtrates, and adjusting the pH value to 6.5;
step two, removing heavy metals: adding 2g of heavy metal sulfydryl chelating agent (2, 3-dimercaptopropanol-1-sulfonic acid) into the solution obtained in the step one, heating to 40 ℃, ultrasonically vibrating for 40min, adjusting the pH value to 6.5 again, adding 1L of absolute ethyl alcohol, standing overnight, and filtering;
step three, purification: and (3) dissolving the filter cake obtained in the second step by using a 6% wt calcium chloride aqueous solution, adding equal volume of absolute ethyl alcohol to precipitate the fucoidan, filtering, washing for 3 times by using the absolute ethyl alcohol, and drying to obtain an extracted product.
Comparative example 1
Extracting with common hot water, and purifying by precipitating with anhydrous ethanol for 2-3 times.
Experimental data
The examples 1-3 and comparative examples were subjected to compositional measurements and the specific data are shown in table 1.
TABLE 1
Composition (I) | Raw material | Example 1 | Example 2 | Example 3 | Comparative example |
Total polysaccharide (%) | 25.1 | 83.9 | 81.6 | 80.4 | 75.3 |
Sulfates of sulfuric acid | 1.25 | 35.8 | 38.7 | 32.8 | 27.3 |
As(mg/kg) | 1.61 | Not detected out | Not detected out | Not detected out | 1.35 |
Pb(mg/kg) | 1.23 | Not detected out | Not detected out | Not detected out | 0.84 |
Wherein the detection method of total polysaccharide is phenol-cysteine-sulfuric acid method, the detection method of sulfate is gelatin-barium chloride method, the detection method of As is described in GB/T5009.11-2003, and the detection method of Pb is described in GB/T5009.11-2003
GB5009.12-2010。
As can be seen from Table 1, the fucoidan extracted by the technical method provided by the invention has the advantages of minimal loss of active sulfate and complete removal of residual heavy metals.
Finally, it should be noted that: the above examples are only for illustrating the technical solutions of the present invention, and are not limited thereto. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: it is to be understood that modifications may be made to the technical solutions described in the foregoing embodiments, or some or all of the technical features may be equivalently replaced, and such modifications or replacements may not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (5)
1. The extraction and purification method of fucoidin is characterized by comprising the following steps:
step one, crude extraction: crushing the dried raw materials by a crusher, and soaking the crushed raw materials in 200mL of acetic acid aqueous solution with the pH value of 4-5 for 2-3 hours; filtering, retaining filtrate, further grinding filter cake into slurry, adding water 8-12 times of the weight of the raw material, heating to 30-40 deg.C, ultrasonic extracting for 30-60min, and filtering; mixing the two filtrates, and adjusting pH to 6.5-7.5;
step two, removing heavy metals: adding a sulfhydryl chelator into the filtrate obtained in the step one, wherein the sulfhydryl chelator is selected from 2, 3-dimercaptopropanol-1-sulfonic acid or 2, 3-dimercaptosuccinic acid, the dosage of the sulfhydryl chelator is 1-3% of the weight of the raw materials, heating to 40-50 ℃, ultrasonically vibrating for 30-60min, adjusting the pH value to 6.5-7.5 again, adding anhydrous ethanol with the weight 8-12 times that of the raw materials, standing overnight, and filtering;
step three, purification: and (3) dissolving the filter cake obtained in the second step by using a calcium chloride aqueous solution, adding equal volume of absolute ethyl alcohol to precipitate the fucoidan, filtering, washing for 3 times by using the absolute ethyl alcohol, and drying to obtain the extracted product.
2. The method for extracting and purifying fucoidan according to claim 1, wherein the raw material is one or more of kelp, sporophyll of undaria pinnatifida, and nemacystus decipiens.
3. The extraction and purification method of fucoidan according to claim 1, wherein the concentration of calcium chloride in the calcium chloride aqueous solution is 5-10% wt.
4. The use of fucoidan prepared by the extraction and purification method according to any one of claims 1 to 3 for preparing a health functional food.
5. Use according to claim 4, wherein the foodstuff is a beverage or an infusible powder.
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CN110981988B (en) * | 2019-12-25 | 2022-05-31 | 无锡先君生物医药有限公司 | Extraction method and application of fucoidin |
CN113278083A (en) * | 2020-05-19 | 2021-08-20 | 杭州鹿扬科技有限公司 | Low molecular weight polysaccharides with antithrombotic activity |
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ES2476250T3 (en) * | 2007-02-23 | 2014-07-14 | Baxter International Inc. | Methods of purification procedure of fucoidane from seaweed extracts |
CN103387622B (en) * | 2013-08-23 | 2015-07-01 | 福建华尔康生物科技有限公司 | Method for extracting fucoidan and removing heavy metals from kelps |
CN107722129A (en) * | 2017-09-18 | 2018-02-23 | 赵方铭 | A kind of method for extracting fucoidin from sea-tangle using ultrasonic wave |
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