CN109121769A - A kind of single-bud cuttage propagation method of Growth anddevelopment - Google Patents

A kind of single-bud cuttage propagation method of Growth anddevelopment Download PDF

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Publication number
CN109121769A
CN109121769A CN201810955241.XA CN201810955241A CN109121769A CN 109121769 A CN109121769 A CN 109121769A CN 201810955241 A CN201810955241 A CN 201810955241A CN 109121769 A CN109121769 A CN 109121769A
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CN
China
Prior art keywords
cuttings
bud
propagation method
growth anddevelopment
cuttage
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CN201810955241.XA
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Chinese (zh)
Inventor
曾丽
李仁清
刘智媛
陆燕青
张邀月
朱晓非
李建青
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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Priority to CN201810955241.XA priority Critical patent/CN109121769A/en
Publication of CN109121769A publication Critical patent/CN109121769A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

Abstract

The invention discloses a kind of single-bud cuttage propagation methods of Growth anddevelopment, comprising the following steps: A, is soaked in water after trimming to Growth anddevelopment middle and lower part branch;B, the branch selection after processing of step A is had into axillary bud and the part not yet sprouted is cut into cuttings;C, cuttings is soaked in rooting powder solution;D, cuttage will be carried out in the hole tray containing matrix through step C treated cuttings, sprinkle profoundly water rear overlay film and take root;E, it after Spike sprouting to be inserted and root long reach 2cm, raises film and moves into nutritive cube;F, it transplants in basin after cuttings covers with root system in nutritive cube to get finished product seedling.Can be simple by single-bud cuttage method, efficient with method of the invention, cuttage material utilization amount is few, saves substrates quantity, the survival rate of Chinese rose is high, cultivation management is simple after cuttage, can provide theoretical foundation for Growth anddevelopment industrialization production, and Post Industrialization production for it lays the foundation.

Description

A kind of single-bud cuttage propagation method of Growth anddevelopment
Technical field
The present invention relates to a kind of single-bud cuttage propagation methods of Growth anddevelopment.
Background technique
Growth anddevelopment (Rosa chinensis minima) is the perennial brushwood of rosaceae Rosa, likes warm and moist, The sufficient environment rich in organic matter, loose ventilative, well-drained subacidity matrix environment of illumination.
Growth anddevelopment is because short and small with plant type, flower-shape is small and exquisite and compact, the florescence is longer, tolerance to cold is relatively strong and ornamental value The features such as high, is commonly used for potting and Cover Plant in the Park, can arrange flower bed or intersperse lawn, can carry out spending hedge production, can also put Windowsill disposed within, balcony, or bonsai is made with mountain stone, dead wood etc..External potting Growth anddevelopment has entered batch production Production, but China is in the selection of matrix, the control of environmental condition, the regulation of production cycle, the control of cuttings quality, Reproduction Optimization of mode etc. there is a problem that fast and efficiently cuttage breeding method has weight to Growth anddevelopment the factorial production It acts on.
The propagation method of Growth anddevelopment often uses cutting propagation and tissue culture propagation two ways, tissue culture propagating production cost Higher, operation sequence is lengthy and jumbled, and the consuming time is more, and the production cycle is longer.The single-bud cuttage breeding of Growth anddevelopment is with when blooming Between it is early, seedling speed is fast, saves substrates quantity, and cuttage material utilization amount is few, the advantages that can effectively keeping parent merit, we More optimize root-inducing powder concentration, substrates quantity and cultivation management mode on this basis, saves labour and production cost The planting scale for expanding Growth anddevelopment simultaneously makes the present invention achieve the purpose that industrialization production.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of Minitype Chinese rose single-bud cuttage breeding;The cottage method can be efficiently sharp With branch, the time is saved, production cost is low, and way to manage is simple, is able to achieve the large-scale production of Growth anddevelopment batch production.
The purpose of the present invention is achieved through the following technical solutions:
The present invention provides a kind of single-bud cuttage propagation methods of Growth anddevelopment, comprising the following steps:
A, branch selection is had into axillary bud and the part not yet sprouted is trimmed to cuttings;
B, cuttings is soaked in rooting powder solution;
C, cuttage will be carried out in the hole tray containing matrix through step B treated cuttings, sprinkle profoundly water rear overlay film and take root;Overlay film It can avoid direct sunlight, be not necessarily to carry out labor management to it during overlay film, can take root after 2-3 weeks;
D, it after Spike sprouting to be inserted and root long reach 2cm, raises film and moves into nutritive cube;
E, it transplants in basin after cuttings covers with root system in nutritive cube to get finished product seedling.
Preferably, in step A, the branch are as follows: play the branch at third blade selected from base portion, and pick out no disease pest Evil, the branch that development is healthy and strong, tissue is substantial.
Preferably, cutting length is 1.5~3cm, and cuttings upper end is apart from 0.3~0.5cm of axillary bud, cuttings lower end distance 1~1.5cm of axillary bud;Cuttings upper end is flat mouth, lower end is 45 ° of angles.
Preferably, in step B, the rooting powder solution is indolebutyric acid (IBA) solution, and concentration is 600~700ppm;Institute Stating soaking time is 3~5s.
Preferably, in step C, before the cuttage, carbendazim solution is added in matrix, the carbendazim solution concentration is 800~900 times 50% of carbendazim solution.
Preferably, the substrate pH is between 5.5~6.0.
Preferably, in step C, when the cuttage, cuttings blade unifies direction, and keeping water content in hole tray is 90%.
Preferably, the matrix in step D, when the cuttings moves into nutritive cube, when lifting on tether panel in hole tray;It moves into Afterwards, in nutritive cube with identical matrix padding and compacting, and root water is poured.
Preferably, in step E, the cuttings is grown after a week in nutritive cube, sprays fertilizer.
Preferably, the fertilizer is compound fertilizer, and usage amount is 1~2g/ alms bowl, is applied once every 20d.
The present invention provides a kind of methods of Minitype Chinese rose single-bud cuttage breeding, are compared to traditional cuttage technique, energy The Characters of Plant Type for enough making full use of Growth anddevelopment has saved materials needed for cuttage, while quickly dipped using root-inducing powder (IBA solution) Method, the time required to greatling save immersion.Compared with existing single-bud cuttage technology, we are lower with quickly dipped method root-inducing powder concentration, Required reagent material can more be saved;On container for plant growth, 128 hole seedling culture hole plates are selected, can more efficiently use space and reduce matrix Materials;In management and maintenance, cuttage efficiency can be effectively improved by looking after cuttings without over head time manpower.
Tests prove that cuttage technique of the present invention survival rate of taking root can reach 92.18%, while to finished product height of seedling 15cm, When having 3~4 section, and it can be trimmed and carry out cuttage as cuttings, not only saved cost while having guaranteed and improving survival Rate reaches the requirement of Growth anddevelopment batch production large-scale production the time required to shortening rootage duration and secondary cuttage.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention, Objects and advantages will become more apparent upon:
Fig. 1 is influence (have carbendazim) of the different IBA concentration to root system average length;
Fig. 2 is influence (no carbendazim) of the different IBA concentration to root system root activity;
Fig. 3 is Growth anddevelopment cuttage process schematic;Wherein, Fig. 3 a is cuttings schematic diagram;Fig. 3 b is after the completion of cuttage Overall pattern;Fig. 3 c is overlay film schematic diagram after cuttage.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field For personnel, without departing from the inventive concept of the premise, several changes and improvements can also be made.These belong to the present invention Protection scope.
The present invention relates to a kind of single-bud cuttage propagation methods of Growth anddevelopment, the described method comprises the following steps:
1, carbendazim solution is added in matrix, adds water and stirs, average mark is loaded in 128 hole hole trays, for use.
2, Growth anddevelopment middle and lower part branch is trimmed, cuts rear branch and is soaked in water immediately, keep base portion wet.
3, for wiping out in the branch picked out in step 2, side branch edible tender branch, choosing has axillary bud and still It does not sprout branch, is cut into cuttings.
4, before cuttage, cuttings is soaked in rooting powder solution after 3~5s and is taken out.
5, by the cuttings cuttage for being moistened with rooting powder solution in step 4 in watering in 128 hole hole trays until irrigate.
6, hole tray is covered with transparent plastic film, can taken root after 2~3 weeks.
7, Spike sprouting to be inserted after root long reaches 2cm, is raised plastic film, is planted in 5cm nutritive cube
8, fertilizer is sprayed after 1 week, is transplanted into after root system covers with nutritive cube in 9cm basin, as finished product seedling.
The present invention preferably 128 hole hole trays.The present invention adheres to resources effective utilization, can accomplish centralization Cultivate administration, Substrates quantity is reduced, management cost is saved.There is no particular/special requirement to the matrix species in the present invention, the matrix can reach guarantor Water holding point breathes freely, and can stablize support cuttings, is maintained under acidic environment, commonly uses cutting medium in the market.
Growth anddevelopment branch is carried out preferably before present invention trimming, selection criteria are as follows: the third blade from base portion, no disease pest Evil, development is healthy and strong, and tissue enriches branch that is hydraulic full and not sprouting.One bud cutting fringe of a leaf is effectively used in the present invention, it can be with The loss of the nutrient and moisture during cuttage is prevented, conducive to taking root and controlling branches and leaves excessive growth.
The middle and lower part of the preferred branch of the present invention is trimmed, and cutting length is about 2~3cm, cuttings biology top distance Axillary bud 0.5cm, apart from axillary bud 1cm, cuttings top is cut into flat mouth for cuttings biology lower part, and angle at 45 ° is cut in lower part, guarantees clip Surfacing, to form callus.
The preferred indolebutyric acid of the present invention (IBA) solution, concentration are 600~700ppm, quickly dipped method, by selecting external source to swash Element promotes cuttings base portion to take root, and accelerates rooting rate.Excellent have selected is tested by carrying out different IBA concentrations versus early period 700ppm is optium concentration, reaches root length longest, the optimal result of quality of rooting.
The carbendazim solution that the concentration of carbendazim solution is 800~900 times 50% is added in the present invention in matrix;The base Matter pH is between 5.5~6.0.
The preferred film covering type management of the present invention guarantees that soil humidity is continuously maintained in hole tray by plastic covering film 80% or more, without carrying out round-the-clock water spray management, water source is saved, labour's investment is reduced;Temperature keeps 20 DEG C substantially More than, meet required optimum temperature of taking root;It is grown under opposite closed environment, can be prevented to a certain extent by externally completely cutting off Only pest and disease damage promotes seedling early growth;Sunlight not only can be sufficiently obtained using transparent membrane, but also its direct projection can be made to avoid the sun It burns at nascent young leaflet tablet.
The present invention preferably applies compound fertilizer, promotes under ground portion sorption enhanced by nutrient contained in compound fertilizer, reaches To strong root strong sprout, the purpose of cuttings viability is improved.There is no stringent particular/special requirement to compound fertilizer in the present invention, the compound fertilizer contains There is basic element needed for plant, the compound fertilizer sold in the market.
A kind of single-bud cuttage propagation method of Growth anddevelopment of the present invention is done into one combined with specific embodiments below Step is discussed in detail, and technology of the invention includes but is not limited to following embodiment:
Embodiment 1:
1,800 times of 50% carbendazim solution is added in matrix, adds water and stirs, average mark is loaded in 128 hole hole trays, to With.Substrate pH is between 5.5~6.0.
2, the middle and lower part dialogue Ke Site branch is trimmed, and is cut rear branch and is soaked in water immediately, keeps base portion wet.
3, for wiping out in the branch picked out in step 2, side branch edible tender branch, choosing has axillary bud and still It does not sprout branch, is cut into 2~3cm cuttings, one leaf of a bud stays 0.3~0.5cm on section, and biology top is cut into flat mouth;Section Under stay 1~1.5cm, angle at 45 ° is cut in biology lower part, guarantees that clip is smooth, as shown in Figure 3a.
4, before cuttage, cuttings is soaked in the IBA solution (rooting powder solution) that concentration is 700ppm after 3-5s and is taken out.
5, the cuttings cuttage that rooting powder solution is moistened in step 4 is irrigated in 128 hole hole trays, as shown in Figure 3b.
6, it is covered hole tray (Fig. 3 c) with transparent plastic film, is taken root after 20d.
7, after cuttage rooting survives, plastic film is raised, temporary planting is in 5cm in nutritive cube.
8, start to apply fertilizer after 1 week, interval 20d sprays compound fertilizer's 1.5g/ basin, is transplanted into 9cm after root system covers with nutritive cube In basin, as finished product seedling.
Use the Chinese rose survival rate of the present embodiment method acquisition for 92.18%.
Embodiment 2:
The method that the present embodiment uses embodiment 1, compares influence of the different IBA concentration to taking root.The IBA concentration of use point Not are as follows: 300ppm, 400ppm, 500ppm, 600ppm, 700ppm.As a result as shown in figure 1 and table 1, using the IBA of 700ppm, root It is average length longest, survival rate highest.
Table 1: different IBA concentration are to cuttings seedling growth effect (having carbendazim)
Comparative example 1:
The method of this comparative example and embodiment 1 is essentially identical, the difference is that only in this comparative example, what step 1 used Carbendazim solution is not added in matrix.
Cutting experiment is as shown in Fig. 2 and table 2, in the case where carbendazim solution is not added, using the IBA of 700ppm, root system Average length longest, survival rate highest, but root long and survival compared with experimental example 2 under the 700ppm IBA concentration of this comparative example Rate is below root long and survival rate under the 700ppm IBA concentration of embodiment 2.
Table 2: different IBA concentration are to cuttings seedling growth effect (no carbendazim)
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow Ring substantive content of the invention.

Claims (10)

1. a kind of single-bud cuttage propagation method of Growth anddevelopment, which comprises the following steps:
A, branch selection is had into axillary bud and the part not yet sprouted is trimmed to cuttings;
B, cuttings is soaked in rooting powder solution;
C, cuttage will be carried out in the hole tray containing matrix through step B treated cuttings, sprinkle profoundly water rear overlay film and take root;
D, it after Spike sprouting to be inserted and root long reach 2cm, raises film and moves into nutritive cube;
E, it transplants in basin after cuttings covers with root system in nutritive cube to get finished product seedling.
2. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1, which is characterized in that in step A, the branch Item are as follows: play the branch at third blade, and no disease and pests harm, the branch that development is healthy and strong, tissue is substantial selected from base portion.
3. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1, which is characterized in that described to insert in step A Fringe is one bud of a leaf, and cutting length is 1.5~3cm, and cuttings upper end is apart from 0.3~0.5cm of axillary bud, and cuttings lower end is apart from armpit 1~1.5cm of bud;Cuttings upper end is flat mouth, lower end is angle.
4. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1, which is characterized in that in step B, the life Root powder solution is indolebutyric acid solution, and concentration is 600~700ppm;The soaking time is 3~5s.
5. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1, which is characterized in that in step C, the skewer Before inserting, carbendazim solution, the carbendazim solution that the carbendazim solution concentration is 800~900 times 50% are added in matrix.
6. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1 or 5, which is characterized in that.
7. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1, which is characterized in that in step C, the skewer When inserting, cuttings blade unifies direction, and keeping water content in hole tray is about 90%.
8. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1, which is characterized in that described to insert in step D Matrix when fringe moves into nutritive cube, when lifting on tether panel in hole tray;After immigration, is filled and pressed with identical matrix in nutritive cube It is real, and pour root water.
9. the single-bud cuttage propagation method of Growth anddevelopment according to claim 1, which is characterized in that described to insert in step E Fringe is grown after a week in nutritive cube, sprays fertilizer.
10. the single-bud cuttage propagation method of Growth anddevelopment according to claim 9, which is characterized in that the fertilizer is multiple Hefei material, usage amount are 1~2g/ alms bowl, are applied once every 20d.
CN201810955241.XA 2018-08-21 2018-08-21 A kind of single-bud cuttage propagation method of Growth anddevelopment Pending CN109121769A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679309A (en) * 2019-11-08 2020-01-14 信阳农林学院 Cutting propagation method of atractylis lancea
CN113475300A (en) * 2021-08-12 2021-10-08 上海应用技术大学 Method for screening cuttings of miniature Chinese roses with different lignification degrees

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679309A (en) * 2019-11-08 2020-01-14 信阳农林学院 Cutting propagation method of atractylis lancea
CN113475300A (en) * 2021-08-12 2021-10-08 上海应用技术大学 Method for screening cuttings of miniature Chinese roses with different lignification degrees

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