CN109081627A - The method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein - Google Patents

The method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein Download PDF

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Publication number
CN109081627A
CN109081627A CN201811148311.7A CN201811148311A CN109081627A CN 109081627 A CN109081627 A CN 109081627A CN 201811148311 A CN201811148311 A CN 201811148311A CN 109081627 A CN109081627 A CN 109081627A
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CN
China
Prior art keywords
protein
foaming agent
mother liquor
agent mother
enzymatic hydrolysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811148311.7A
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Chinese (zh)
Inventor
黎冰
高玉峰
杜杰
马文昊
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Southeast University
Hohai University HHU
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Southeast University
Hohai University HHU
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Publication date
Application filed by Southeast University, Hohai University HHU filed Critical Southeast University
Priority to CN201811148311.7A priority Critical patent/CN109081627A/en
Publication of CN109081627A publication Critical patent/CN109081627A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C04CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04BLIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B40/00Processes, in general, for influencing or modifying the properties of mortars, concrete or artificial stone compositions, e.g. their setting or hardening ability
    • C04B40/0028Aspects relating to the mixing step of the mortar preparation
    • C04B40/0039Premixtures of ingredients
    • CCHEMISTRY; METALLURGY
    • C04CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04BLIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2103/00Function or property of ingredients for mortars, concrete or artificial stone
    • C04B2103/42Pore formers

Abstract

The present invention discloses a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein, includes the following steps: that 1) material protein, alkali protease and water are placed in container and is uniformly mixing to obtain mixed liquor, and adjusts pH value to 9~13;2) mixed liquor for obtaining step 1) is reacted in heating and thermal insulation, is readjusted pH value after a certain period of time to 9~13, is repeated several times and can be prepared by foaming agent mother liquor.This method is convenient and efficient, can significantly improve the production efficiency of air bubble mix light-textured soil foaming agent mother liquor, which has good practical value.

Description

The method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein
Technical field
The present invention relates to a kind of methods for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein, main to use In producing foaming agent mother liquor, it is related to foaming agent technical field.
Background technique
Foaming agent is the indispensable former material for preparing the civil engineerings light material such as air bubble mix light-textured soil and foam concrete Material, the superiority and inferiority of foaming agent performance are to influence the deciding factor of air bubble mix light-textured soil and foam concrete performance, and prepare The compound foaming agent of function admirable needs first to prepare foaming agent mother liquor.
The existing method for producing protein-based foaming agent mother liquor is to utilize alkali, acid or salts substances direct hydrolysis egg mostly The amount of white matter, alkali, acid or salts substances that this method needs to be added is often more, and hydrolyzes the time it takes often Longer, hydrolysis efficiency is very low, is unfavorable for the pilot production of foaming agent.Alkali protease is urged as a kind of common protein hydrolysis Agent, can suitable pH value and at a temperature of quick catalysis hydrolyze different kinds of proteins, by breaks down proteins be small molecule peptide chain With the substances such as amino acid, this is consistent with the product of conventional hydrolysis method, and advantage is that efficiently, protein hydrolysis can be saved significantly on The time it takes.
Summary of the invention
Technical problem: foaming agent is prepared using basic protein enzymatic hydrolysis protein the object of the present invention is to provide a kind of The method of mother liquor, this method can substantially reduce hydrolysis time with catalyzing hydrolysis material protein, solve existing basic hydrolysis, sour water Solve the problem low with the methods of salt hydrolysis hydrolysis efficiency.
Technical solution: foaming agent mother liquor is prepared using basic protein enzymatic hydrolysis protein the present invention provides a kind of Method, method includes the following steps:
1) material protein, alkali protease and water are placed in container and are uniformly mixing to obtain mixed liquor, and adjust pH value to 9 ~13;
2) the mixed liquor heating and thermal insulation obtained step 1) reacts, and reaction readjusts pH value to 9~13 after a certain period of time, It is repeated several times and can be prepared by foaming agent mother liquor.
Wherein:
Contain activator in mixed liquor described in step 1), which is inorganic reagent, and it is ionized in aqueous solution Cation out only has Ca2+、Mn2+、Mg2+、Zn2+Or Fe2+, the quality of the activator is the 0.01%~3% of the quality of water.
The cation that activator preferably ionizes out in aqueous solution only has Ca2+Inorganic reagent.
The alkali protease is Alcalase.
The material protein is vegetable protein or animal protein.
The material protein is ox hoof-and-horn meal, sugar-free grains, soy meal, pig hair meal end or gelatin.
The alkali protease be Maxacal L alkali protease, 300000 alkali protease of Maxacal P, Novozyme 680T alkali protease, Alcalase 2.0T basic protein or subtilopeptidase A.
Reagent used in adjusting pH value described in step 1) is basic mineral reagents, more preferably, using sodium hydroxide or Calcium hydroxide.
The quality of the alkali protease is the 0.05%~3% of material protein quality.
The quality of the water is 10~50 times of material protein quality.
The mixed liquor for obtaining step 1) described in step 2) is reacted in heating and thermal insulation, refers to that in temperature be 30~60 DEG C Heating and thermal insulation reacts in thermostat water bath.
The reaction readjusts pH value to 9~13 after a certain period of time, refers to that 10~30min of interval readjusts pH Value.
Described being repeated several times is 1~6 time.
The utility model has the advantages that compared with the prior art, the advantages of the present invention are as follows:
1. the present invention takes full advantage of the catalytic action that alkali protease hydrolyzes material protein, hydrolysis is significantly improved Efficiency saves the energy while reducing hydrolysis time;
2. the present invention can substantially reduce compared with the methods of traditional basic hydrolysis, sour water solution and salt hydrolysis and produce foaming agent Time required for mother liquor, this is of great significance for improving the production efficiency of foaming agent mother liquor.
Detailed description of the invention
Fig. 1 is the pH value of solution in parallel test with the change curve of hydrolysis time.
Specific embodiment
Technical solution of the present invention is described further below with reference to specific implementation case.
In control environment, enzyme can make macromolecular peptide chain be decomposed into average molecular matter with the hydrolysis of catalytic proteins The substances such as lesser polypeptide are measured, polypeptide can further be hydrolyzed to the substances such as the smaller dipeptides of relative molecular mass again, if reaction Continue, dipeptides can be decomposed into amino acid again.The fracture of this substantial peptide bond of hydrolytic process, enzyme are simultaneously not involved in reaction, only It is catalysis reaction.The present invention uses hydrolysis by novo material protein, and alkali protease is exactly utilized for material protein The catalytic action of hydrolysis can accelerate hydrolysis rate by control pH value and temperature, shorten hydrolysis time, efficiently convenient, can The production efficiency for significantly improving foaming agent, the pilot production particularly suitable for foaming agent mother liquor.
Embodiment 1:
A method of foaming agent mother liquor being prepared using basic protein enzymatic hydrolysis protein, this method includes following step It is rapid:
1) 10g ox hoof-and-horn meal, 0.3gMaxacal L alkali protease are added in beaker, the stirring of 500g water is added later Uniformly obtain mixed liquor, later be added 15g calcium chloride (activator), be slowly added to later sodium hydroxide adjust mixed liquor pH value to 13;
2) beaker is put into the thermostat water bath that set temperature is 60 DEG C and is heated, every the pH of 30min measurement mixed liquor Value, and be slowly added to sodium hydroxide and adjust mixed liquor pH value to 13, until hydrolysis time reaches 2h to get foaming agent mother liquor.
Embodiment 2:
1) 20g sugar-free grains, 300000 alkali protease of 0.01g Maxacal P are added in beaker, 200g water is added later It is uniformly mixing to obtain mixed liquor, 0.02g magnesium sulfate (activator) is added later, sodium hydroxide is slowly added to later and adjusts mixed liquor PH value is to 9;
2) beaker is put into the thermostat water bath that set temperature is 30 DEG C and is heated, every the pH of 10min measurement mixed liquor Value, and be slowly added to sodium hydroxide and adjust mixed liquor pH value to 9, until hydrolysis time reaches 1 hour to get foaming agent mother liquor.
Embodiment 3:
1) 10g soy meal, 0.2g Novozyme 680T alkali protease are added in beaker, 400g water is added later and stirs It mixes and uniformly obtains mixed liquor, 2g calcium chloride (activator) is added later, be slowly added to sodium hydroxide later and adjust mixed liquor pH value To 10.5;
2) beaker is put into the thermostat water bath that set temperature is 50 DEG C and is heated, every the pH of 30min measurement mixed liquor Value, and be slowly added to sodium hydroxide and adjust mixed liquor pH value to 10.5, until hydrolysis time reaches 1 hour to get foaming agent mother Liquid.
Embodiment 4:
1) 10g pig hair meal end, 0.3g Alcalase 2.0T alkali protease are added in beaker, 300g water is added later Be uniformly mixing to obtain mixed liquor, later be added calcium hydroxide (not only be used as activator, but also as alkali) adjust mixed liquor pH value to 11.5;
2) beaker is put into the thermostat water bath that set temperature is 50 DEG C and is heated, every the pH of 20min measurement mixed liquor Value, and be slowly added to calcium hydroxide and adjust mixed liquor pH value to 11.5, until hydrolysis time reaches 1h to get foaming agent mother liquor.
Embodiment 5:
1) 10g gelatin, 0.1g subtilopeptidase A are added in beaker, addition 200g water is uniformly mixing to obtain mixed later Liquid is closed, calcium hydroxide (not only as activator, but also as alkali) is slowly added to later and adjusts mixed liquor pH value to 12.5;
2) beaker is put into the thermostat water bath that set temperature is 40 DEG C and is heated, every the pH of 30min measurement mixed liquor Value, and be slowly added to calcium hydroxide and adjust mixed liquor pH value to 12.5, until hydrolysis time reaches 30min to get foaming agent mother Liquid.
Embodiment 6
In order to preferably embody advantage of the invention, parallel test is carried out below and is compared: gelatin dosage 10g, hydrogen-oxygen Change calcium dosage 0.4g, subtilopeptidase A dosage 0.1g, preparation method provided by the invention and traditional preparation methods are carried out Comparative illustration:
1,10g gelatin, 0.1g subtilopeptidase A and 0.4g calcium hydroxide are added in beaker 1# and 2#, in beaker 3# With addition 10g gelatin and 0.4g calcium hydroxide in 4#, 200g water is added in 1#, 2#, 3# and 4# beaker, stirs evenly;
2, it measures the pH value of solution in 1#, 2#, 3# and 4# beaker respectively using pH meter, and records;
3,1#, 2#, 3# and 4# beaker are put into the thermostat water bath that set temperature is 50 DEG C and are heated;
4, every 30min, first the solution of 1#, 2#, 3# and 4# beaker is stirred evenly, then measures the pH and note of solution respectively Record, until hydrolysis time reaches 5 hours;
5, respectively draw 1#, 2#, 3# and 4# beaker solution ph versus time curve (as shown in Figure 1): 1# with The decrease speed of pH value of solution in 2# beaker is significantly faster than that the solution of 3# and 4# beaker, this is because gelatin meeting in hydrolytic process It generates carboxyl and neutralizes the OH in solution, and the speed hydrolyzed is faster, and the generating rate of carboxyl is faster, the pH value of solution With regard to the faster of decline;In addition, the catalytic action of enzyme is the most aobvious when can also be seen that pH value is in 10~13 ranges by curve It writes, it is reasonable that this, which illustrates that initial pH value is arranged in 10~13 ranges,.
Present invention is mainly used for foaming agent mother liquor is prepared, a small amount of alkali protease need to only be added, so that it may significantly improve The hydrolysis efficiency of protein, while any adverse consequences will not be generated, it is time-saving while also reducing compared to conventional method The consumption of the energy.Therefore, this method has very high practical value, significantly improves the production efficiency of foaming agent.

Claims (10)

1. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein, it is characterised in that: this method The following steps are included:
1) material protein, alkali protease and water are placed in container and are uniformly mixing to obtain mixed liquor, and adjust pH value to 9~ 13;
2) the mixed liquor heating and thermal insulation for obtaining step 1) reacts, and reaction readjusts pH value to 9~13 after a certain period of time, repeats It can be prepared by foaming agent mother liquor for several times.
2. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: containing activator in mixed liquor described in step 1), which is inorganic reagent, and it is electric in aqueous solution The cation separated out only has Ca2+、Mn2+、Mg2+、Zn2+Or Fe2+, the quality of the activator is the 0.01%~3% of the quality of water.
3. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: the alkali protease is Alcalase.
4. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: the material protein is vegetable protein or animal protein.
5. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: reagent used in adjusting pH value described in step 1) is basic mineral reagents.
6. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: the quality of the alkali protease is the 0.05%~3% of material protein quality.
7. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: the quality of the water is 10~50 times of material protein quality.
8. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: the mixed liquor heating and thermal insulation for step 1) being obtained described in step 2) reacts, refer to that in temperature be 30~60 DEG C Thermostat water bath in heating and thermal insulation reaction.
9. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: the reaction readjusts pH value to 9~13 after a certain period of time, refer to that 10~30min of interval is readjusted PH value.
10. a kind of method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein as described in claim 1, It is characterized by: described being repeated several times is 1~6 time.
CN201811148311.7A 2018-09-29 2018-09-29 The method for preparing foaming agent mother liquor using basic protein enzymatic hydrolysis protein Pending CN109081627A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109320122A (en) * 2018-11-06 2019-02-12 河南城建学院 A kind of preparation method of foam concrete foamer
CN110156370A (en) * 2019-05-05 2019-08-23 东南大学 A kind of compound foaming agent

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5135451A (en) * 1974-09-20 1976-03-25 Ajinomoto Kk Kayoseitanpaku no seizoho
CN102173644A (en) * 2011-01-27 2011-09-07 西南科技大学 Pea protein concrete foaming agent and preparation method thereof
CN102964083A (en) * 2012-10-26 2013-03-13 安徽艾柯泡塑股份有限公司 Modified soy protein foaming agent for concrete
CN103169942A (en) * 2011-12-24 2013-06-26 栾清杨 Enzymolysis method for preparing velvet antler collagen
CN105621928A (en) * 2016-02-18 2016-06-01 陕西科技大学 Cement foaming agent prepared from scrap leather and method for preparing cement foaming agent
CN106587702A (en) * 2016-11-10 2017-04-26 董晓 Preparation method of stable-type odor-free low-density foam concrete foamer

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5135451A (en) * 1974-09-20 1976-03-25 Ajinomoto Kk Kayoseitanpaku no seizoho
CN102173644A (en) * 2011-01-27 2011-09-07 西南科技大学 Pea protein concrete foaming agent and preparation method thereof
CN103169942A (en) * 2011-12-24 2013-06-26 栾清杨 Enzymolysis method for preparing velvet antler collagen
CN102964083A (en) * 2012-10-26 2013-03-13 安徽艾柯泡塑股份有限公司 Modified soy protein foaming agent for concrete
CN105621928A (en) * 2016-02-18 2016-06-01 陕西科技大学 Cement foaming agent prepared from scrap leather and method for preparing cement foaming agent
CN106587702A (en) * 2016-11-10 2017-04-26 董晓 Preparation method of stable-type odor-free low-density foam concrete foamer

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109320122A (en) * 2018-11-06 2019-02-12 河南城建学院 A kind of preparation method of foam concrete foamer
CN110156370A (en) * 2019-05-05 2019-08-23 东南大学 A kind of compound foaming agent

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