CN109044555A - Intervention method establishes after dog autologous blood clots embolic cerebral infarction thrombolysis intracranial hemorrhage transformation model and its application - Google Patents
Intervention method establishes after dog autologous blood clots embolic cerebral infarction thrombolysis intracranial hemorrhage transformation model and its application Download PDFInfo
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Abstract
The present invention relates to a kind of intervention methods to establish after dog autologous blood clots embolic cerebral infarction thrombolysis intracranial hemorrhage transformation model and its application.Include the following steps: the judgement of the preparation of thrombus, the preparation of Cerebral Infarction Model, thrombolysis and bleeding result.Dog hemorrhagic conversion model of the present invention has the advantage that one, the brain structure of beasle dog are similar to the mankind, and less, compared to toys such as mouse, preferably clinical conversion is may be implemented in relevant experimental result to the variation of cerebrovascular diameter.Secondly, toy experiment in, the variation of monitor cerebral blood flow is mostly carried out using laser Doppler flowmetry, to judge the occlusion of blood vessel and lead to again；And goldstandard DSA whether unobstructed as clinical judgment blood flow, the injection of thrombus can be instructed, accurately embolism is carried out, and provides the whether effective positive evidence of thrombolysis.Thirdly, intervention operation be used as a minimal invasive techniques, some other unnecessary operation can be reduced to the maximum extent and damaged, to improve the accuracy and reliability of experimental result.
Description
Technical field
The invention belongs to preclinical medicine technical fields, and in particular to a kind of intervention method establishes dog autologous blood clots embolic cerebral infarction
It intracranials hemorrhage after dead thrombolysis transformation model and its application.
Background technique
In recent years, acute cerebral infarction seriously affects the health of the mankind, and about 10~40% patient understands nature after cerebral infarction
Conversion, which is formed, intracranials hemorrhage, its lethality and disability rate is further aggravated.As intravenous injection tissue plasminogen swashs
Living agent (rtPA) and intravascular be intracavitary to take bolt to treat acute cerebral infarction gradually increasing in clinical application, these treatment methods exist
While restoring ischemic tissue of brain blood perfusion, the ratio that conversion of intracranialing hemorrhage occurs is also increased.Especially in thromboembolism treatment
In, if the time for restoring ischemic tissue of brain blood perfusion is more than 4.5h, conversion of intracranialing hemorrhage increases than regular meeting conspicuousness.
Conversion of intracranialing hemorrhage can be it is asymptomatic, but be influence cerebral infarction prognosis independent hazard factor, will lead to
Mortality and disability rate obviously increase.Therefore, the mechanism for conversion of intracranialing hemorrhage after cerebral infarction thrombolysis and progress are understood in depth
It is corresponding to intervene, it is particularly important for improving Patients with Cerebral Infarction prognosis.At present about correlation of intracranialing hemorrhage after cerebral infarction thrombolysis
Experimental study have been achieved for part progress, but using toys such as mouse as experimental animal, due to toy dissection knot
The notable difference of structure and human body, and the difference of modeling method and human body pathogenesis, related experiment result cannot achieve effectively
Clinic conversion.
Summary of the invention
For the defects in the prior art, the present invention is intended to provide a kind of intervention method establishes dog autologous blood clots embolic cerebral infarction
It intracranials hemorrhage after dead thrombolysis transformation model.The structure of the cerebrovascular and brain parenchym based on dog and the mankind are relatively similar, target organ compared with
Greatly, so as to preferably simulating the generation and development of human body diseases.
For this purpose, the invention provides the following technical scheme:
In a first aspect, the present invention provides transformation model of intracranialing hemorrhage after a kind of dog autologous blood clots embolic cerebral infarction thrombolysis
Method for building up includes the following steps: S1: the preparation of thrombus: using dog as experimental animal, sterilizing after bilateral inguinal area preserved skin
Drape punctures left femoral vein merging sheath for vein haemospasia and makes thrombus；S2: the preparation of Cerebral Infarction Model: right side is punctured
Femoral artery is placed in catheter sheath, introduces the cerebral angiography assessment that vertebral artery conduit carries out system through femoral artery sheath；Later successively
Thrombus is injected into arteria cerebri media, when aortocranial angiography confirms that arteria cerebri media entirely shuts, conduit is connected into pressurization life
Salt water is managed, ipsilateral internal carotid 2h is blocked；S3: recombinant tissue-type's fibrinolysin thrombolysis: is given by vein in post-stroke 4.5h
Former activator rtPA, dosage 2mg/kg, 10% load of intravenous injection, the vein of residue 90% maintain 60min for the first time,
Whether the blood vessel of polysography observation later leads to again；S4: CT scan, general pathology and HE dyeing grade-judging the judgement of bleeding result: are used
The disconnected generation for whether having conversion of intracranialing hemorrhage.Preferably, using beasle dog as experimental animal.
Preferably, in S1, the preparation of thrombus is specifically included: S101: being extracted dog autologous vein blood, is taken upper layer after centrifugal treating
Blood plasma；S102: being added the physiological saline for containing 100 unit of thrombin in upper plasma, and injection is led immediately after mixing
Guan Zhong；S103: every dog gives 2500 units heparins through femoral vein, and is infused with physiological saline intravenous infusion and maintain venous channel, 1.5~
The thrombus for solidifying and being precipitated moisture is taken out from conduit after 2.5h, is placed in physiological saline and rinses repeatedly, later with water suction yarn
Cloth blots；S104: production thrombus item, and it is trimmed to default size, thrombus item is placed in the sterile device equipped with physiological saline later
It is spare in ware.
Preferably, in S101: the condition of centrifugal treating includes: that temperature is 3~5 DEG C, and revolving speed is 3500~4500rpm, when
Between be 5~10min；The extraction amount of venous blood and the volume ratio of the physiological saline containing 100 unit of thrombin are (5~10): 1.
Preferably, the condition of centrifugal treating are as follows: temperature is 4 DEG C, revolving speed 4000rpm, time 10min；Venous blood
Extraction amount and the volume ratio of the physiological saline containing 100 unit of thrombin are 8:1.
Preferably, in S104: making diameter respectively and be the thrombus of 1.4mm and 1.7mm, and thrombus item is trimmed and is grown into
The thrombus of 5mm.
Preferably, in S2, the preparation of Cerebral Infarction Model is specifically included: S201: being punctured right common femoral artery and is placed in catheter sheath, warp
5F femoral artery sheath introduces vertebral artery conduit under digital subtraction angiography machine real-time fluoroscopic and path profile guiding, and conduit is surpassed
Selectivity is inserted to bilateral internal carotid artery, row positive side position radiography, then the super choosing of conduit is recorded brain blood to left vertebral artery normotopia radiography
Pipe structure feature, whether there is or not abnormal anatomies, observe the performance of arterial phase and venous phase, and measure left side internal carotid artery cervical part of esophagus, brain
The blood vessel diameter of middle artery, arteria cerebri anterior；After the cerebral angiography assessment of the system of progress, the super choosing of conduit is inserted to left side
Internal carotid ascent stage about 2cm；S202: the thrombus item merging of 1.4mm diameter is equipped with to inject in the 2mL syringe of contrast medium and is led
Pipe, once thrombus enters conduit, uses the 5mL syringe equipped with physiological saline instead and gives discontinuity pressure and slowly inject, radiography
It confirms the distal end of first thromboembolism arteria cerebri media M1 and then injects the thrombus of 1.7mm diameter in the same way
To the proximal end of middle cerebral artery M 1 segment；S203: when aortocranial angiography confirms that middle cerebral artery M 1 segment entirely shuts, by conduit
Pressure physiological saline is connected, ipsilateral internal carotid 2h is blocked.
Second aspect, application of the method provided by the invention in acute cerebral infarction simulated experiment.
Applicant has found by numerous studies: compared with the small animal models such as mouse, dog hemorrhagic conversion mould prepared by the present invention
Type has the advantage that one, the brain structure of beasle dog are similar to the mankind, and the variation of cerebrovascular diameter is little, compared to mouse etc.
Preferably clinical conversion may be implemented in toy, relevant experimental result.Secondly, toy experiment in, mostly using laser it is more
General Le blood flowmeter carrys out the variation of monitor cerebral blood flow, to judge the occlusion of blood vessel and lead to again；And DSA is as clinical judgment blood flow
No unobstructed goldstandard can instruct the injection of thrombus, carry out accurately embolism, and provide the whether effective directly card of thrombolysis
According to.Thirdly, intervention operation be used as a minimal invasive techniques, some other unnecessary operation can be reduced to the maximum extent and damaged,
Such as the operation of opening cranium being difficult to avoid that or cervical incision vascular ligation in toy Cerebral Infarction Model, to improve experiment knot
The accuracy and reliability of fruit.Fourth, cerebral infarction is mostly prepared with foreign matters such as bolt lines in mouse Cerebral Infarction Model, machine of falling ill
There are larger differences with thromboembolism for system.The present invention can be very good the morbidity of simulation human body midbrain infarction with dog autologous blood clots
Situation.
Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description
Obviously, or practice through the invention is recognized.
Detailed description of the invention
Fig. 1 is the schematic diagram that arterial-venous sets pipe in the embodiment of the present invention；
Fig. 2 is in the embodiment of the present invention for judging the CT scan figure of bleeding result；
Fig. 3 is in the embodiment of the present invention for judging the general pathology figure of bleeding result；
Fig. 4 is in the embodiment of the present invention for judging the HE colored graph of bleeding result.
Specific embodiment
The embodiment of technical solution of the present invention is described in detail below.Following embodiment is only used for more clearly
Illustrate technical solution of the present invention, therefore be only used as example, and not intended to limit the protection scope of the present invention.
Experimental method in following embodiments is unless otherwise specified conventional method.Examination as used in the following examples
Material is tested, is to be commercially available from conventional reagent shop unless otherwise specified.Quantitative test in following embodiment, extremely
Three repeated experiments are set less, and data are the average value or mean+SD of three repeated experiments.
Embodiment
It establishes after dog autologous blood clots embolic cerebral infarction thrombolysis to intracranial hemorrhage the present embodiment provides a kind of intervention method and converts mould
Type includes the following steps:
S1: the preparation of thrombus
Using beasle dog as experimental animal, the sterile drape after bilateral inguinal area preserved skin punctures left femoral vein and is placed in sheath
Pipe makes thrombus for vein haemospasia.Beasle dog autologous vein blood 4mL is extracted, is placed in centrifuge tube, 4000rpm is centrifuged at 4 DEG C
After 10min, upper plasma is taken, the guiding catheter that physiological saline of the 0.5mL containing 100 unit of thrombin is mixed and injected immediately is added
In.Then every dog gives 2500 units heparins through femoral vein, and is infused with physiological saline intravenous infusion and maintain venous channel (2mL/
min).The thrombus for solidifying and being precipitated moisture is taken out from conduit after about 2h, is placed in use after rinsing repeatedly in physiological saline and inhale
Water gauze blots, and then production diameter is the thrombus of 1.4mm and 1.7mm respectively, and thrombus item is trimmed to the blood for growing into 5mm
Thrombus item is finally placed in spare in the sterile vessel equipped with physiological saline by bolt item.
S2: the preparation of Cerebral Infarction Model
It punctures right common femoral artery and is placed in catheter sheath.Vertebral artery conduit is introduced through 5F femoral artery sheath to make in Digital Subtraction blood vessel
Under shadow machine (DSA) real-time fluoroscopic and path profile guiding, conduit super-selective is inserted to bilateral internal carotid artery, row positive side position radiography,
Again by the super choosing of conduit to left vertebral artery normotopia radiography, record cerebral vessel structures feature, whether there is or not abnormal anatomies, observe arterial phase and
The corresponding performance of venous phase, and with measurement left side internal carotid artery cervical part of esophagus, arteria cerebri media, arteria cerebri anterior blood vessel diameter.?
After the cerebral angiography assessment of the system of progress, the super choosing of conduit is inserted to left side internal carotid artery ascent stage about 2cm.Then, will
The thrombus item merging of 1.4mm diameter, once thrombus enters conduit, uses dress instead equipped with conduit is injected in the 2mL syringe of contrast medium
There is the 5mL syringe of physiological saline and give discontinuity pressure and slowly inject, radiography confirms to move in first thromboembolism brain
The distal end of arteries and veins M1 and then the thrombus of another 1.7mm diameter is injected into the close of middle cerebral artery M 1 segment in the same way
End.Cerebral angiography confirms that arteria cerebri media entirely shuts.Aortocranial angiography confirms that middle cerebral artery M 1 segment entirely shuts, then
Conduit is connected into pressure physiological saline, blocks ipsilateral internal carotid 2h.The schematic diagram that arterial-venous sets pipe is as shown in Figure 1.
S3: thrombolysis
Referring to clinical application method and method, recombinant tissue-type plasminogen is given by vein in post-stroke 4.5h
Activator (rtPA), the 2mg/kg that dosage is, 10% load of intravenous injection, the vein of residue 90% maintain for the first time
60min.Whether the blood vessel of polysography observation later leads to again.
S4: the judgement of bleeding result
The hair for conversion of intracranialing hemorrhage is judged whether there is using CT scan (Fig. 2), general pathology (Fig. 3) and HE dyeing (Fig. 4)
It is raw.
The result of study of the present embodiment shows: after two autologous blood clots of injection, left side middle cerebral arterial occludes immediately.
After vein gives rtPA, effective revascularization can be realized.In addition, CT scan is as the head for detecting bleeding in clinical position
Choosing strategy, CT scan have apparent high density area as the result is shown, prompt the generation of hemorrhagic conversion.General pathology assessment confirms brain soldier
In after 4.5h give rtPA after there is apparent hemorrhagic conversion.HE dyes visible a large amount of erythrocyte extravasations to brain parenchym with pyknosis
Core.
Comparative example
This comparative example, which provides a kind of intervention method, to be established after dog autologous blood clots embolic cerebral infarction thrombolysis to intracranial hemorrhage and converts mould
Type includes the following steps:
S1: the preparation of thrombus
Using beasle dog as experimental animal, the sterile drape after bilateral inguinal area preserved skin punctures left femoral vein and is placed in sheath
Pipe makes thrombus for vein haemospasia.Beasle dog autologous vein blood 4mL is extracted, is placed in centrifuge tube, 4000rpm is centrifuged at 4 DEG C
After 10min, upper plasma is taken, the guiding catheter that physiological saline of the 0.5mL containing 100 unit of thrombin is mixed and injected immediately is added
In.Then every dog gives 2500 units heparins through femoral vein, and is infused with physiological saline intravenous infusion and maintain venous channel (2mL/
min).The thrombus for solidifying and being precipitated moisture is taken out from conduit after about 2h, is placed in use after rinsing repeatedly in physiological saline and inhale
Water gauze blots, and then production diameter is the thrombus of 1.4mm and 1.7mm respectively, and thrombus item is trimmed to the blood for growing into 5mm
Thrombus item is finally placed in spare in the sterile vessel equipped with physiological saline by bolt item.
S2: the preparation of Cerebral Infarction Model
It punctures right common femoral artery and is placed in catheter sheath.Vertebral artery conduit is introduced through 5F femoral artery sheath to make in Digital Subtraction blood vessel
Under shadow machine (DSA) real-time fluoroscopic and path profile guiding, conduit super-selective is inserted to bilateral internal carotid artery, row positive side position radiography,
Again by the super choosing of conduit to left vertebral artery normotopia radiography, record cerebral vessel structures feature, whether there is or not abnormal anatomies, observe arterial phase and
The corresponding performance of venous phase, and with measurement left side internal carotid artery cervical part of esophagus, arteria cerebri media, arteria cerebri anterior blood vessel diameter.?
After the cerebral angiography assessment of the system of progress, the super choosing of conduit is inserted to left side internal carotid artery ascent stage about 2cm.Then, will
The thrombus item merging of 1.4mm diameter, once thrombus enters conduit, uses dress instead equipped with conduit is injected in the 2mL syringe of contrast medium
There is the 5mL syringe of physiological saline and give discontinuity pressure and slowly inject, radiography confirms to move in first thromboembolism brain
The distal end of arteries and veins M1 and then the thrombus of another 1.7mm diameter is injected into the close of middle cerebral artery M 1 segment in the same way
End.Cerebral angiography confirms that arteria cerebri media entirely shuts.Aortocranial angiography confirms that middle cerebral artery M 1 segment entirely shuts, then
Conduit is connected into pressure physiological saline, blocks ipsilateral internal carotid 2h.
S3: thrombolysis
Referring to clinical application method and method, recombinant tissue-type plasminogen is given by vein in post-stroke 3h and is swashed
Being (rtPA), the 2mg/kg that dosage is, 10% load of intravenous injection, the vein of residue 90% maintain 60min for the first time.
Whether the blood vessel of polysography observation later leads to again.
S4: the judgement of bleeding result
The generation for conversion of intracranialing hemorrhage is judged whether there is using CT scan, general pathology and HE dyeing.
The result of study of this comparative example shows: after two autologous blood clots of injection, left side middle cerebral arterial occludes immediately.
After vein gives rtPA, effective revascularization can be realized.CT scan is even visible a small amount of as the result is shown without high-density region
It is dispersed in dotted density regions.General pathology assessment is shown no obvious abnormalities.The brain tissue necrosis of HE result notification portion, inflammatory cell
Infiltration.
Compared with the toy experimental model of traditional cerebral infarction, dog hemorrhagic conversion model prepared by the present invention has many elder generations
Into property.There are many limitations for the toy experimental model of cerebral infarction.Most researchers pass through insertion bolt line mechanical plug method,
Or direct injection thrombus, or the rodents focal cerebral ischemia in rats such as mouse is prepared with rose-red photochemical reaction.It is transporting
On the basis of these modeling methods, generation is induced to intracranial hemorrhage using spontaneous hypertension or hyperglycemia rodent.Make
For mechanical plug model, middle cerebral artery occlusion model prepared by line brush and the pathogenesis of human body cerebral infarction are not inconsistent.?
When photochemical reaction establishes Cerebral Infarction Model, embolus is mainly made of blood platelet, moreover, opening in the operating process of this method
Cranium operation be it is unavoidable, may result in cerebrospinal fluid leakage, influence experimental result.Rodent embolus injection model with
The pathogenesis of patients with cerebral apoplexy is more similar, but needs to ligature external carotid artery after injecting, and can cause hemodynamic responses.Such as
Fruit Direct perforantes arteria cerebri media thrombin injection, it is likely that will lead to injury of blood vessel.In addition to this, using spontaneous hypertension
Or hyperglycemia rodent carry out hemorrhagic conversion experimental study be it is not rigorous, these zooscopies can simulate certain spies
Fixed clinical condition such as the patient of hypertension or diabetes, but is not enough to simulate after clinically cerebral infarction caused by thromboembolism treatment
It intracranials hemorrhage conversion.Above-mentioned these features and actual clinical situation have very big difference.In addition, the arteria carotis system of rodent
There is very big differences for the anatomical structure and the mankind of system and brain tissue, and mouse is agyria animal and internal carotid has more one
Wing jaw arterial branch, cerebral infarction experiment is carried out with experimental dog can preferably simulate the occurrence and development of disease of human body.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
It can be combined in any suitable manner in one or more embodiment or examples.In addition, without conflicting with each other, the skill of this field
Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples
It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, modifies, replacement and variant.
Claims (8)
1. a kind of method for building up for transformation model of intracranialing hemorrhage after dog autologous blood clots embolic cerebral infarction thrombolysis, which is characterized in that
Include the following steps:
S1: the preparation of thrombus
Using dog as experimental animal, the sterile drape after bilateral inguinal area preserved skin punctures left femoral vein merging sheath and is used for
Vein haemospasia makes thrombus；
S2: the preparation of Cerebral Infarction Model
It punctures right common femoral artery and is placed in catheter sheath, the cerebral angiography for introducing vertebral artery conduit progress system through femoral artery sheath is commented
Estimate；Thrombus is successively injected into arteria cerebri media later, when aortocranial angiography confirms that arteria cerebri media entirely shuts, by conduit
Pressure physiological saline is connected, ipsilateral internal carotid 2h is blocked；
S3: thrombolysis
Recombinant tissue-type plasminogen activator rtPA, dosage 2mg/kg are given by vein in post-stroke 4.5h,
The vein of 10% load of intravenous injection for the first time, residue 90% maintains 60min, and whether the blood vessel of polysography observation later leads to again；
S4: the judgement of bleeding result
The generation for conversion of intracranialing hemorrhage is judged whether there is using CT scan, general pathology and HE dyeing.
2. the method for building up of transformation model according to claim 1 of intracranialing hemorrhage, it is characterised in that:
In the S1, the preparation of thrombus is specifically included:
S101: dog autologous vein blood is extracted, takes upper plasma after centrifugal treating；
S102: being added the physiological saline for containing 100 unit of thrombin in the upper plasma, after mixing injection guiding immediately
In conduit；
S103: every dog gives 2500 units heparins through femoral vein, and is infused with physiological saline intravenous infusion and maintain venous channel, 1.5~
The thrombus for solidifying and being precipitated moisture is taken out from conduit after 2.5h, is placed in physiological saline and rinses repeatedly, later with water suction yarn
Cloth blots；
S104: production thrombus item, and it is trimmed to default size, thrombus item is placed in the sterile vessel equipped with physiological saline later
In, it is spare.
3. the method for building up of transformation model according to claim 2 of intracranialing hemorrhage, it is characterised in that:
In the S101:
The condition of centrifugal treating includes: that temperature is 3~5 DEG C, and revolving speed is 3500~4500rpm, and the time is 5~10min；It is described quiet
The extraction amount of arteries and veins blood and the volume ratio of the physiological saline for containing 100 unit of thrombin are (5~10): 1.
4. the method for building up of transformation model according to claim 3 of intracranialing hemorrhage, it is characterised in that:
The condition of the centrifugal treating are as follows: temperature is 4 DEG C, revolving speed 4000rpm, time 10min；The extraction of the venous blood
Amount and the volume ratio of the physiological saline for containing 100 unit of thrombin are 8:1.
5. the method for building up of transformation model according to claim 2 of intracranialing hemorrhage, it is characterised in that:
In the S104:
Production diameter is the thrombus of 1.4mm and 1.7mm respectively, and thrombus item is trimmed to the thrombus for growing into 5mm.
6. the method for building up of transformation model according to claim 5 of intracranialing hemorrhage, it is characterised in that:
In the S2, the preparation of Cerebral Infarction Model is specifically included:
S201: puncturing right common femoral artery and be placed in catheter sheath, vertebral artery conduit is introduced through 5F femoral artery sheath, in Digital Subtraction blood vessel
Under contrast machine real-time fluoroscopic and path profile guiding, conduit super-selective is inserted to bilateral internal carotid artery, row positive side position radiography, then incite somebody to action
For the super choosing of conduit to left vertebral artery normotopia radiography, record cerebral vessel structures feature, whether there is or not abnormal anatomies, observe arterial phase and vein
The performance of phase, and measure the blood vessel diameter of left side internal carotid artery cervical part of esophagus, arteria cerebri media, arteria cerebri anterior；In the system of progress
Cerebral angiography assessment after, the super choosing of conduit is inserted at left side internal carotid artery ascent stage 2cm；
S202: the thrombus item merging of 1.4mm diameter being equipped in the 2mL syringe of contrast medium and injects conduit, once thrombus enters
Conduit uses the 5mL syringe equipped with physiological saline instead and gives discontinuity pressure and slowly injects, and radiography confirms first thrombus bolt
It fills in the distal end of arteria cerebri media M1 and then the thrombus of 1.7mm diameter is injected into middle cerebral artery M 1 segment in the same way
Proximal end；
S203: when aortocranial angiography confirms that middle cerebral artery M 1 segment entirely shuts, conduit is connected into pressure physiological saline, is blocked up
Fill in ipsilateral internal carotid 2h.
7. the method for building up for transformation model of intracranialing hemorrhage described in any one according to claim 1~6, it is characterised in that:
The dog selects beasle dog.
8. application of any one of claim 1~7 the method in acute cerebral infarction simulated experiment.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109816650A (en) * | 2019-01-24 | 2019-05-28 | 强联智创（北京）科技有限公司 | A kind of target area recognition methods and its system based on two-dimentional DSA image |
| CN112889746A (en) * | 2021-01-15 | 2021-06-04 | 首都医科大学宣武医院 | Multi-sinus combined cerebral venous thrombosis animal model and construction method thereof |
| CN113506634A (en) * | 2021-07-15 | 2021-10-15 | 南京易爱医疗设备有限公司 | Brain simulation system |
| CN115362987A (en) * | 2022-10-26 | 2022-11-22 | 首都医科大学宣武医院 | Preparation method and application of thrombus |
-
2018
- 2018-08-30 CN CN201811005739.6A patent/CN109044555A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109816650A (en) * | 2019-01-24 | 2019-05-28 | 强联智创（北京）科技有限公司 | A kind of target area recognition methods and its system based on two-dimentional DSA image |
| CN112889746A (en) * | 2021-01-15 | 2021-06-04 | 首都医科大学宣武医院 | Multi-sinus combined cerebral venous thrombosis animal model and construction method thereof |
| CN112889746B (en) * | 2021-01-15 | 2023-01-20 | 首都医科大学宣武医院 | Multi-sinus combined cerebral venous thrombosis animal model and construction method thereof |
| CN113506634A (en) * | 2021-07-15 | 2021-10-15 | 南京易爱医疗设备有限公司 | Brain simulation system |
| CN113506634B (en) * | 2021-07-15 | 2024-04-09 | 南京易爱医疗设备有限公司 | Brain Simulation System |
| CN115362987A (en) * | 2022-10-26 | 2022-11-22 | 首都医科大学宣武医院 | Preparation method and application of thrombus |
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Application publication date: 20181221 |