CN109019873A - A kind of salt tolerant efficient denitrification type water process microorganism formulation - Google Patents
A kind of salt tolerant efficient denitrification type water process microorganism formulation Download PDFInfo
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- CN109019873A CN109019873A CN201811041599.8A CN201811041599A CN109019873A CN 109019873 A CN109019873 A CN 109019873A CN 201811041599 A CN201811041599 A CN 201811041599A CN 109019873 A CN109019873 A CN 109019873A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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Abstract
The invention discloses a kind of salt tolerant efficient denitrification type water process microorganism formulations, are made of following several active microorganism types: bacillus subtilis (Bacillus subtilis), Pseuomonas denitrifican (Pseudomonas denitrificans), Halomonas (Halomonas), Halophiles (Halophile).Microorganism formulation of the invention still has good denitrification ability under high salt conditions, solves limitation of the hypersaline environment to traditional biological denitrogenation method；It suitable for the denitrogenation processing of salinity content high-salt wastewater below 8%, has a extensive future, there is good social benefit.
Description
Technical field
The invention belongs to microbial environment protecting technical fields, more particularly to a kind of micro- life of salt tolerant efficient denitrification type water process
Object preparation.
Background technique
High-salt wastewater refers to that salt content is greater than the waste water of 1% (w/v), part even more than 20%.Its is from a wealth of sources, ingredient
Complexity contains a large amount of Cl-、SO4 2-、Na+And Ca2+Etc. soluble inorganic salt ions.The processing method of high-salt wastewater is main at present
Including physical chemistry method, biochemical method and combinations thereof technique.Wherein physical chemistry method includes evaporation, UF membrane, ion-exchange electrolyte etc.,
Though treatment effect is good but all there is the problems such as at high cost, energy consumption is big mostly, physical chemistry method is seriously constrained practical with high salt useless
Application in water process.And relative to physical chemistry method, biochemical method but has treatment effeciency is high, economy is friendly, convenient for management etc.
Advantage, thus it is also the most extensive in practical applications.But the inorganic salts of high-salt wastewater middle and high concentration can inhibit even to poison activity
Microorganism in sludge destroys its Bacterial community, so that it is lost degradation capability, therefore biochemical method is in the processing of high-salt wastewater
Application be limited by very large.In recent years, people gradually adapt to the salt ring of high-salt wastewater by taming ordinary activated sludge
Border, obtains the microorganism of certain salt resistance ability with this, but there are salt tolerant degree is not high, treatment effeciency is not high, the punching of salt resistance degree
The problems such as hitting poor ability.Therefore find a kind of not only salt tolerant and there is the microbial bacterial agent of high degrading activity, just at solution this
The key of one problem.
Thermophilic salt, salt-durable microbe have metabolic function identical with traditional microbiological, can give birth in different salinity environment
It is long, using it is a variety of include that hydrocarbons and a variety of poisonous and harmful compounds carry out metabolism, have and utilize a variety of electron acceptors,
Extracellular polymers are generated, the characteristics such as chemical substance murder by poisoning in environment are lowered.It, directly will be from using modern biology method with means
It is separately cultured in hypersaline environment and thermophilic salt salt tolerant bacteria agent that massive amplification culture obtains investment high-salt wastewater biochemical treatment system
Sewage treatment is carried out, provides an effective solution approach in the processing of high-salt wastewater for bioanalysis.
Ammonia nitrogen, nitrate and nitroso nitrogen are the important process indexs of nitrogenous effluent, and it is main to handle nitrogenous effluent at present
Method is traditional biological denitrogenation technology.The basic principle of biological denitrificaion be under the action of microorganism by sewage organic nitrogen and
Ammoniacal nitrogen is converted into N2Process, including two reaction process of nitrification and denitrification.Nitration reaction is aerobic micro- by a group autotrophy
Biology is completed, and is specifically completed respectively by two kinds of bacterium of Nitrosomas and Nitromonas in two stages.The first step is will by Nitrosomas
NH4 +It is oxidized to NO2 -, second step is by Nitromonas by NO2 -It is further oxidized to NO3 -.Anti-nitration reaction is simultaneous by a group heterotroph
Property anaerobe complete, refer under anaerobic or hypoxia condition, denitrifying bacteria is by NO2 -And NO3 -It is reduced to the mistake of nitrogen
Journey.Traditional biological denitrification process mainly includes the techniques such as A/O method, A2/O, oxidation ditch, CASS, ANNAMOX, MSBR.However it is traditional
For bio-denitrification technology while its extensive use, there is also some shortcomings.Such as nitrifier is autotroph, growth is slow
Slowly, therefore in activated sludge can not be competed with heterotrophicy bacteria；Influence of the biochemical system vulnerable to external environment, impact resistance
It is poor, cause denitrification effect undesirable etc..Therefore, how effectively to improve the denitrification ability of biochemical system and be again one and urgently solve
Certainly the problem of.
Summary of the invention
To solve the problems in the background art, the present invention provides a kind of salt tolerant and the composite microbials of efficient denitrification
Object preparation.
The technical scheme adopted by the invention is that such: a kind of salt tolerant efficient denitrification type water process microorganism formulation,
It is made of following several active microorganism types: bacillus subtilis (Bacillus subtilis), Pseuomonas denitrifican
(Pseudomonas denitrificans), Halomonas (Halomonas), Halophiles (Halophile).
Microorganism formulation living bacteria count amount >=1011CFU/g。
Wherein, above-mentioned bacillus subtilis, Pseuomonas denitrifican, Halomonas, Halophiles mass ratio are preferably 2:2:2:
1。
Wherein above-mentioned water process microorganism formulation further includes catalyst iron powder, and the content of iron powder is in 1%~5% (w/t)
Between.
Wherein, it is glucose 5g/L, (NH that above-mentioned water process, which grows culture solution ingredient used with microorganism formulation,4)2SO4
2g/L, K2HPO40.5g/L, MgSO40.2g/L, NaCl 10g/L.
Wherein, waste iron filing of the iron powder from steel plant, Metal Processing Factory, gained, partial size after further crushing grinding
Size is between 1-3 mm.
It is worth noting that in view of the particularity of microorganism field, each strain ratio described above and its closing
The composition of formula is only applicable to before adding；After this compound formulation puts into waste water to be processed, since the growth ability of microorganism is different
In addition the interaction between microorganism will lead to, final respective ratio is not also identical, this is depended on when treating wastewater, respectively
The activity of a strain.That is, it is nonsensical for talking microorganism ratio in use.
The present invention has following advantage by applicant's research repeatedly and practice, discovery:
1) the water process microorganism system as composed by bacillus subtilis, Pseuomonas denitrifican, Halomonas, Halophiles
Agent still has good denitrification ability under high salt conditions, solves limitation of the hypersaline environment to traditional biological denitrogenation method.
2) iron powder has good adsorption properties due to biggish specific surface area, can not only be preferably direct with microorganism
Contact, can also be adsorbed on surface for organic matter, to promote the formation of surface biological film；As semiconductor, iron powder can promote
Electron transmission between microorganism, to effectively improve nitrification, denitrifying rate, on the other hand, iron powder is by micro-reduction
Bioavailable ferrous iron is formed, nitrite can be not only restored and generate gaseous nitrogen, reduce because the accumulation of nitrite is to micro-
The toxic action of biology, but also grown and active nutrient as microorganism is improved.
3) on the one hand a certain amount of iron powder that microorganism formulation is added being urged as the denitrification ability for improving biochemical system
On the other hand agent also achieves the secondary use of waste iron filing, reduce resource waste and metallic pollution.
4) present invention is suitable for the denitrogenation processing of salinity content high-salt wastewater below 8%, has a extensive future, has
Good social benefit.
Detailed description of the invention
It is described in further detail below in conjunction with attached drawing and embodiments of the present invention
Fig. 1 is denitrification effect figure of the microorganism formulation under different salt concentration conditions.Curve S1 is 1% salinity in figure,
Curve S2 is 3% salinity, and curve S3 is 5% salinity, and curve S4 is 8% salinity, and curve S5 is 10% salinity.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies specific material proportion, process conditions and its result described in example and is merely to illustrate the present invention, without that should will not limit
The present invention described in detail in claims processed.
(1) preparation of microorganism formulation
The bacillus subtilis that inclined-plane saves is added in basal medium LB culture solution, culture solution ingredient mentions for yeast
Take object 5g/L, peptone 10g/L, NaCl 10g/L.It is put into the isothermal vibration shaking table activation culture 12h of 30 DEG C, 180r/min, so
It is forwarded in the fermentor containing denitrogenation culture medium and expands culture by 5% inoculum concentration again afterwards, culture solution ingredient is grape
Sugared 5g/L, (NH4)2SO42g/L, K2HPO40.5g/L, MgSO42g/L, NaCl 10g/L.The condition of culture of fermentor is 30
DEG C, speed of agitator 180r/min, 0.4~0.6vvm of ventilatory capacity.Stop fermentation after fermented and cultured 48h.Fermentation liquid is collected,
Low-temperature centrifugation 10min under 6000r/min takes the resuspension of precipitating sterile phosphate buffer that purpose bacteria suspension can be obtained, finally
Bacteria suspension is lyophilized by way of freeze-drying to obtain bacterium powder.Same method respectively obtains Pseuomonas denitrifican, salt unit cell
Bacterium, Halophiles bacterium powder so that freeze-dried vaccine powder living bacteria count be not less than 1011cfu/g.By 4 kinds of bacterium powder according to bacillus subtilis
Bacterium, Pseuomonas denitrifican, Halomonas and Halophiles mass ratio be 2:2:2:1 mixing, mix, the mixing of bacterium powder can be obtained
Then the iron powder that 1~5% (w/t) is added in obtained mixture is finally obtained purpose microorganism formulation by object.
(2) denitrification effect of the microorganism formulation under different salt concentration conditions
Measurement of water process microorganism formulation described in embodiment under different salt concentration conditions to ammonia nitrogen removal ability,
Specific implementation step is as follows:
By microorganism formulation by 2% inoculum concentration access fresh salinity (in terms of NaCl) be respectively 1%, 3%, 5%,
8%, in 10% denitrogenation culture medium, wherein ammonia-nitrogen content carries out oscillation training in 540mg/L or so in the shaking table of 30 DEG C of 180rpm
It supports.Nonvaccinated culture medium carries out the experiment under equal conditions as blank control.In 6h, 12h, for 24 hours, 36h and 48h take on a small quantity
Culture solution is centrifuged 5min at 8000rpm, and supernatant is taken to measure its ammonia nitrogen concentration.
As a result as shown in Fig. 1, when ammonia nitrogen initial concentration is 500mg/L, salinity 1%, 3%, 5%, 8%,
When 10%, its ammonia nitrogen concentration is reduced to 50mg/L, 70mg/L, 100mg/L, 180mg/L, 330mg/L after reacting 48h.
After microorganism formulation 6h is added, ammonia-nitrogen content variation is less likely to because thallus has just started the too low institute of growing mycelia concentration
It causes.After 12h, growth is in logarithmic phase, and viable count greatly increases, and ammonia nitrogen removal frank significantly improves.Especially after 48h,
Ammonia nitrogen removal frank under 1% salinity reaches as high as 90%.And with the raising of salinity, especially under 10% salinity,
The ammonia nitrogen removal frank of reaction system is obviously suppressed, and only 34%.Nevertheless, microorganism formulation used in the present invention exists
Under the conditions of salinity is 10% below, good ammonia nitrogen removal effect is still shown.
(3) denitrification effect of the microorganism formulation in practical high-salt wastewater
Processing water sample is certain petrochemical industry high-salt wastewater, and the high-salt wastewater salinity is in 3%~5% or so, COD 800
Between~1200mg/L, ammonia nitrogen is between 150~250mg/L, and pH is 7.5~9.0 or so.It, will by 1%~2% inoculum concentration
Microorganism formulation is added in the high-salt wastewater, 30 DEG C or so Air Exposure 5 days, daily timing sampling measure its ammonia-nitrogen content change
Change, this is experimental group.Efficient denitrification microbial inoculum (the ammonia nitrogen removal frank: 95% for separately taking certain producer to produce；Living bacteria count: 5.9*
109cfu/g；The tolerance of salinity≤3%) it is inoculated into this high-salt wastewater according to identical inoculum concentration, it is aerated 5 days under the same terms, often
Its ammonia-nitrogen content is measured by sampling in its timing, this is control group.As a result as shown in the table:
Experimental group on day 1, just starts to grow in high-salt wastewater since microorganism formulation is added to, and cell density is little,
Therefore ammonia-nitrogen content has no too big variation in reaction system.After 2nd day, with mushrooming out for microorganism, thallus substantially increases
More, ammonia nitrogen removal frank starts to significantly rise, and ammonia nitrogen removal frank can reach 85%.And control group, due to higher salinity
To the inhibiting effect of thallus, thallus can not normal growth, cause its ammonia nitrogen removal effect lower, ammonia nitrogen removal frank is only up to
25%, hence it is evident that lower than the ammonia nitrogen removal effect of experimental group.It can be seen that salt tolerant denitrogenation-type microorganism formulation of the present invention
It is with the obvious advantage relative to other common denitrogenation microbial inoculum significant effects in the denitrogenation processing of practical high-salt wastewater.
Claims (4)
1. a kind of salt tolerant efficient denitrification type water process microorganism formulation, it is characterised in that: by following several active microorganism kinds
Class composition: bacillus subtilis (Bacillus subtilis), Pseuomonas denitrifican (Pseudomonas
Denitrificans), Halomonas (Halomonas), Halophiles (Halophile).
2. a kind of salt tolerant efficient denitrification type water process microorganism formulation as described in claim 1, it is characterised in that: microorganism
Preparation living bacteria count amount >=1011CFU/g。
3. a kind of salt tolerant efficient denitrification type water process microorganism formulation as described in claim 1, it is characterised in that: above-mentioned withered
Careless bacillus, Pseuomonas denitrifican, Halomonas, Halophiles mass ratio are preferably 2:2:2:1.
4. a kind of salt tolerant efficient denitrification type water process microorganism formulation as described in claim 1, it is characterised in that: further include
Catalyst iron powder, the content of iron powder is between 1%~5% (w/t).
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Cited By (2)
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| CN110241039A (en) * | 2019-05-25 | 2019-09-17 | 天津金辰博科环保科技发展有限公司 | A kind of salt tolerant efficient denitrification type water process microorganism formulation |
| CN111847658A (en) * | 2020-06-16 | 2020-10-30 | 广东省石油与精细化工研究院 | Composite microbial inoculum for enhancing nitrogen and phosphorus removal of sewage as well as preparation method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110241039A (en) * | 2019-05-25 | 2019-09-17 | 天津金辰博科环保科技发展有限公司 | A kind of salt tolerant efficient denitrification type water process microorganism formulation |
| CN111847658A (en) * | 2020-06-16 | 2020-10-30 | 广东省石油与精细化工研究院 | Composite microbial inoculum for enhancing nitrogen and phosphorus removal of sewage as well as preparation method and application thereof |
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