CN109006723A - The method that intertidal zone aufwuch is sampled using portable sampler - Google Patents

The method that intertidal zone aufwuch is sampled using portable sampler Download PDF

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Publication number
CN109006723A
CN109006723A CN201810615042.4A CN201810615042A CN109006723A CN 109006723 A CN109006723 A CN 109006723A CN 201810615042 A CN201810615042 A CN 201810615042A CN 109006723 A CN109006723 A CN 109006723A
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China
Prior art keywords
plastic film
barnacle
vacuum
piston
sampling
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CN201810615042.4A
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Chinese (zh)
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CN109006723B (en
Inventor
丰美萍
毕远新
严涛
王伟定
梁君
张亚洲
周珊珊
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Zhejiang Marine Fisheries Research Institute
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Zhejiang Marine Fisheries Research Institute
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Priority to CN201810615042.4A priority Critical patent/CN109006723B/en
Publication of CN109006723A publication Critical patent/CN109006723A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K80/00Harvesting oysters, mussels, sponges or the like

Abstract

The invention discloses the methods sampled using portable sampler to intertidal zone aufwuch, one plastic film is first covered on to the rock surface positioned at intertidal zone, and it is bonded in the edge seal of plastic film on rock, air gap is formed between plastic film and rock surface at this time;Vacuum plant is connect with the gas outlet on plastic film with check valve again, starts vacuum plant then to extract the air between plastic film and rock surface out, plastic film at this time is close to rock surface;When Plastic film surface is attached with barnacle, a sampling sleeve is entangled into the barnacle for needing to sample, then makes to sample formation vacuum in sleeve, so that barnacle be sucked in sampling sleeve.The present invention not only can greatly facilitate the sampling of barnacle, it is ensured that sample the integrality of barnacle.

Description

The method that intertidal zone aufwuch is sampled using portable sampler
Technical field
The present invention relates to intertidal organism studying technological domains, utilize portable sampler between tide more particularly, to a kind of Method with aufwuch sampling.
Background technique
With the fast development of port and pier construction, marine aquaculture, oil-gas mining etc., the attachment of marine fouling organism Problem becomes more and more prominent, seriously hampers the development and utilization of marine resources, and people is forced to have to spend largely Manpower and material resources are gone to eliminate and be prevented and treated.In numerous marine fouling organisms, barnacle with its adaptability to environment, and become compared with Representative one kind.
Barnacle is most important dynamic in the most common marime fouling animal and the detection of material anti-pollution and evaluation test Object model.There is extremely hard shell outside the adult human body of barnacle, also secret out of a kind of first raw rubber of barnacle of viscosity, this glue contains There are many biochemical and extremely strong bonding forces, to make it have extremely strong adhesive ability.In ocean research, it is often necessary to Band carries out biological sampling between removing moisture, and especially carries out whole sampling to barnacle.Due to adult barnacle can a large amount of epiphytisms in tide Between on wet and slippery rock, and its adhesive strength is high, therefore, sample collector can not the manually direct sample from rock, from And cause its sampling very difficult.People are usually to use the hardware tool of existing perching knife etc by shell and its meat of inside Under body is shoveled from rock together, but this method easily causes the broken of barnacle shell, to influence to sample effect.
Summary of the invention
The purpose of the present invention is to solve existing when being sampled to the barnacle being attached on the rock of intertidal zone Sampling is difficult, is easy to keep barnacle shell broken to influence the problem of sampling effect, provides and a kind of utilizes portable sampler pair The method of intertidal zone aufwuch sampling, not only can greatly facilitate the sampling of barnacle, it is ensured that sample the integrality of barnacle.
To achieve the goals above, the invention adopts the following technical scheme:
A method of intertidal zone aufwuch is sampled using portable sampler, is included the following steps:
A. a plastic film is covered on the rock surface positioned at intertidal zone, and is bonded in the edge seal of plastic film On rock, air gap is formed between plastic film and rock surface at this time;
B. vacuum plant is connect with the gas outlet on plastic film with check valve, starts vacuum plant then to extract out Air between plastic film and rock surface, plastic film at this time are close to rock surface;
C. when Plastic film surface is attached with barnacle, a sampling sleeve is entangled into the barnacle for needing to sample, then makes to sample Vacuum is formed in sleeve, so that barnacle be sucked in sampling sleeve.
In order to facilitate sampling, the present invention first covers a plastic film in the rock surface for being located at intertidal zone, and passes through pumping The mode of vacuum makes plastic film be close to rock surface.In this way, the barnacle young can adhere on plastic film, while avoiding moulding Expect that film and rock surface disengage.When the young of barnacle is grown into the degree of suitable sampling, sampling personnel can be to rattan Pot easily samples.It is attached to due to barnacle on soft plastic film, and there is no inhale between plastic film and rock Attached power, therefore, sampling personnel perhaps directly can remove barnacle or by barnacle together with plastic film on plastic film It removes.In particular, a sampling sleeve is first entangled the barnacle for needing to sample in sampling by the present invention, then make to sample in sleeve Vacuum is formed, relies on draught head at this time, that is, is convenient to rapidly entirely suck barnacle in sampling sleeve, thus can be effectively Avoid the breakage of barnacle shell, it is ensured that sample the integrality of barnacle.
Preferably, the sampling upper end closed of sleeve, lower end are open-minded, constant pressure is equipped in the upper surface of sampling sleeve Chamber is equipped with several hydraulic cylinders axially extending along sampling sleeve in the lateral wall of sampling sleeve, and hydraulic cylinder is in sampling sleeve Lateral surface on be arranged side by side, piston boit is equipped in the hydraulic cylinder, the lower end that piston boit stretches out hydraulic cylinder is equipped with taper Bur is equipped with through hole in the closed upper surface of hydraulic cylinder, and the upper end of the through hole penetrates through constant pressure chamber, the lower end of through hole Hydraulic cylinder is penetrated through, the positioning pressure spring for compressing piston boit is equipped on the top of hydraulic cylinder inner cavity, the constant pressure is intracavitary to be filled with Hydraulic oil, when sampling sleeve entangles the barnacle for needing to sample, the taper bur for sampling the piston column end of all around stems is successive With the rock contact around barnacle, thus make each taper bur height and rock corresponding points height be adapted, taper bur A continuous tearing hole of circle is formed on the plastic film of attachment barnacle;When sampling formation vacuum in sleeve, adhere to barnacle Plastic film, by tear fracture, samples in sleeve at tearing hole so that barnacle be made to be inhaled into together with plastic film.
Each hydraulic cylinder of the invention is connected by through hole with constant pressure chamber, to make each hydraulic cylinder and constant pressure chamber Hydraulic fluid pressure is always consistent.In this way, when sampling sleeve entangles the barnacle for needing to sample, due to barnacle surrounding rock Surface, which has, to be uneven, and therefore, the taper bur for sampling the piston column end of all around stems part can be first and around barnacle Rock contact, corresponding piston boit is under pressure retrogressing at this time, and it is intracavitary that the hydraulic oil in the hydraulic cylinder is then squeezed into constant pressure, Into taper bur not in contact in the hydraulic cylinder of rock, and then the piston boit in the hydraulic cylinder is made to move forward, piston column end Taper bur contradict rock surface, until final all taper burs equably contradict the surface of rock injustice.It bores at this time Shape bur forms a continuous tearing hole of circle on the plastic film of attachment barnacle;When sampling formation vacuum in sleeve, attachment The plastic film of barnacle, by tear fracture, samples in sleeve at tearing hole so that barnacle be made to be inhaled into together with plastic film.? That is sampling sleeve of the invention entangle need sample barnacle when, can simultaneously attachment barnacle plastic film on shape At a continuous tearing hole of circle, to be conducive to improve the efficiency of barnacle sampling, and being uneven for rock surface can be eliminated Adverse effect.
Preferably, the plastic film is EVA heat-shrinkable film.
It is known that EVA material have closedcells structure, and do not absorb water, be moisture-proof, water resistance it is good, simultaneously also With good corrosion resistance, and it is nontoxic, tasteless, pollution-free, therefore, on the one hand ocean will not be polluted, be made simultaneously The coarse and with higher surface energy of Plastic film surface, thus be conducive to the attachment of barnacle.Especially since rock surface is not It is the plane that can be flattened, therefore, when plastic film is covered on rock surface, plastic film will form many folds. Plastic film of the invention is EVA heat-shrinkable film, and therefore, before vacuum extraction, we can first heat plastic film, thus So that plastic film is rapidly formed contraction, and then can effectively eliminate the fold of plastic film, so that it is become flat and endure.Work as vacuum holding When setting the air between extraction plastic film and rock, plastic film can be made to be flattened on rock surface, and then be conducive to barnacle Attachment and growth.
Preferably, demarcation plate is equipped in sampling sleeve, to be separated out the vacuum pumping chamber on top in sampling sleeve With the accommodating cavity of lower part, piston and reseting pressuring spring are equipped in vacuum pumping chamber, the piston is equipped in the downside close to demarcation plate The limited post in reseting pressuring spring is protruded into, the reseting pressuring spring lower end compresses demarcation plate, and upper end compresses piston, is equipped on demarcation plate The air inlet for penetrating through accommodating cavity and vacuum pumping chamber is equipped with the connector of calming the anger of perforation vacuum pumping chamber on the upper surface of sampling sleeve, The connector of calming the anger is connected with inflator, and the connector of calming the anger is also other to be connected to bleeder pipe, and angle valve is equipped on bleeder pipe, when beating When inflator gradually injects air to vacuum pumping chamber, the elastic force of piston overpowers reseting pressuring spring and gradually move down, until limited post against Demarcation plate and position;When opening angle valve is communicated with the atmosphere vacuum pumping chamber, reseting pressuring spring driving piston quickly moves up reset, from And parital vacuum is formed in the part moment in vacuum pumping chamber between piston and demarcation plate, and then barnacle is sucked into sampling set In the accommodating cavity of cylinder lower end.
Reseting pressuring spring makes piston be in the top of vacuum pumping chamber, and when needing to sample barnacle, sampling personnel can be by inflating Cylinder is pressed into air into the vacuum pumping chamber of sampling sleeve, to drive the elastic force of piston overpowers reseting pressuring spring and move down, until limit Position column is positioned against demarcation plate, and the air pressure in vacuum pumping chamber at this time is greater than external air pressure.That is, limit at this time Column, which had not only played the role of one, positions piston, but also plays the guiding role of reset spring, and reset spring is avoided to be squeezed When generate skew.When the personnel of sampling open angle valve, the air in vacuum pumping chamber can promptly be discharged by bleeder pipe, multiple Position pressure spring can then drive piston quickly to move up, and form vacuum in the vacuum pumping chamber between piston and demarcation plate at this time, correspondingly, hold It sets intracavitary air to be promptly pumped by the air inlet on demarcation plate in the vacuum pumping chamber of piston lower portion, accommodating cavity at this time The vacuum state of moment a kind of is inside formed, and then barnacle can be sucked in accommodating cavity.
Preferably, forming clearance fit between the piston and sampling sleeve, the periphery of piston is equipped with circular ring shape Sealed groove, the steel sealing ring with opening, the two ends difference of steel sealing ring opening are equipped in sealed groove Equipped with bonding jumper, the bonding jumper mutual dislocation at two ends, the lateral surface of steel sealing ring, which reclines, samples the inner sidewall of sleeve.
It should be noted that sealing ring made of rubber is typically provided on existing cylinder piston, to realize piston to gas The sealing of working chamber in cylinder.Therefore, sealing ring will form biggish damping action to piston when moving, and piston is made to be difficult quickly to move It is dynamic.And the sealed groove of circular ring shape is arranged in piston of the invention on the circumferential surface, and setting has opening in sealed groove Steel sealing ring, we can make the outer diameter of steel sealing ring be greater than the internal diameter for sampling sleeve.When installing in this way, we can inwardly be squeezed Steel sealing ring is pressed, falls into steel sealing ring hot spring in sealed groove, so that piston be made to be convenient to be packed into sampling sleeve. After piston enters in sampling sleeve, steel sealing ring struts outward by spontaneous elastic force, to make the outer of steel sealing ring Side, which reclines, samples the inner sidewall of sleeve, and realization piston is substantially sealed off to sampling sleeve, at this time steel sealing ring opening both ends Bonding jumper mutually overlap, thus be conducive to improve steel sealing ring opening sealing effect.Since steel seals Frictional force between ring and sampling set medial wall of the tube will be much smaller than between sealing ring made of rubber and sampling set medial wall of the tube Therefore frictional force is conducive to fast moving for piston, and then can realize the moment vacuum of accommodating cavity.Due to steel sealing ring with The gap sampled between sleeve is minimum, and therefore, in the moment that piston quickly moves up, the air in vacuum pumping chamber is entered by piston Amount to accommodating cavity is minimum, will not influence the vacuum sample effect of sampling sleeve.
Preferably, being further added by step d after step c: adhesive layer is arranged at the edge of a vacuum freshness retaining film, then will The vacuum freshness retaining film is covered on rock surface exposed after barnacle samples, and the edge of the vacuum freshness retaining film is bonded in plastics On film, then repeatedly step b, makes plastic film be close to rock surface again.
When we remove sampling barnacle together with plastic film, the plastic film being covered on rock will form one A hole, the present invention first covers a soft vacuum freshness retaining film at the hole, and is bonded in the edge of vacuum freshness retaining film On plastic film, the air being then then turned between vacuum plant extraction plastic film and rock surface makes plastic film again Adsorbed close is on a rock surface.Since vacuum freshness retaining film has very strong toughness, after vacuumizing, external atmospheric pressure effect On vacuum freshness retaining film, so that vacuum freshness retaining film be made tightly to be adsorbed on rock, at the same time, the edge of vacuum freshness retaining film is tight It is thickly crimped on adhesive layer, so that the excellent sealing between vacuum freshness retaining film and original plastic film can be realized.
Preferably, being equipped with connecting tube on a plastic film, the limit plate of circular ring shape is equipped on the inner sidewall of connecting tube, The inner hole of the limit plate is to form the gas outlet, and the check valve includes the mobile column being arranged in gas outlet, is being moved The outer end of the dynamic close connection tube opening of column is integrally provided with outer limit disk, in the inner end of mobile column integrally provided with truncated cone-shaped Interior limit disk, the edge for limiting disk outside are equipped with the pressurization film of circular cone annular made of PVC film, the circle of the mobile column Cylinder is equipped with the outgassing groove axially through interior limit disk, nut cap is threaded in the opening of connecting tube, in step b In, the exhaust tube of vacuum plant is first sealedly connected on to the opening of connecting tube, then starts vacuum plant, at this time in limit plate The air pressure of side is greater than the air pressure in outside, and mobile column is displaced outwardly together with inside and outside limit disk, and interior limit disk is against limit plate Inside, the air between plastic film and rock are discharged by gas outlet;When being formed between plastic film and rock surface When vacuum, vacuum plant is closed, and remove the exhaust tube at connecting tube, the air pressure on the inside of limit plate at this time is less than the gas in outside Pressure, mobile column moves inward together with inside and outside limit disk, outer limit disk against the outside of limit plate, pressurization film then with limit The lateral surface of plate fits closely, to form dual-seal to gas outlet, finally covers nut cap again, plastic film can be made to continue It is close to rock surface in ground.
Since the opening of connecting tube is threaded with nut cap, good guarantor can be formed to the check valve in connecting tube Shield, and can make the exhaust tube of vacuum plant that can easily be tightly connected with connecting tube by hex union nut.In addition, rotary table The interior limit disk of shape, which facilitates, squeezes into gas outlet for flexible check valve, and inside and outside limit disk can limit in the axial direction at this time Determine the movement of check valve.When vacuum plant just starts to vacuumize, the air pressure on the inside of limit plate is greater than the air pressure in outside, mobile column It is displaced outwardly under the influence of air pressure together with inside and outside limit disk, interior limit disk is against the inside of limit plate, outer limit Disk is then detached from limit plate.Since mobile column is equipped with the outgassing groove through interior limit disk, plastic film and rock it Between air can by outgassing groove pass through limit plate discharge;When forming vacuum between plastic film and rock surface, Vacuum plant is closed, and removes the exhaust tube at connecting tube, the air pressure on the inside of limit plate at this time is less than the air pressure in outside, mobile column It is moved inward under the action of external pressure together with inside and outside limit disk, outer limit disk is blocked against the outside of limit plate Gas outlet, interior limit disk are then detached from limit plate, and the lateral surface of pressurization film and limit plate at this time fits closely, and are increased and are blocked The sealing effect of gas outlet.
Therefore, the invention has the following beneficial effects: the samplings that not only can greatly facilitate barnacle, it is ensured that samples barnacle Integrality.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of present invention sampling sleeve.
Fig. 2 is a kind of structural schematic diagram of the plastic film at connecting tube.
In figure: 1, putty film 11, connecting tube 12, limit plate 121, gas outlet 13, nut cap 3, sampling sleeve 31, Constant pressure chamber 32, hydraulic cylinder 321, through hole 33, piston boit 331, taper bur 34, positioning pressure spring 35, demarcation plate 351, air inlet 36, piston 361, limited post 362, sealed groove 363, steel sealing ring 364, bonding jumper 37, reset Pressure spring 38, connector 39 of calming the anger, bleeder pipe 391, angle valve 4, inflator 5, mobile column 51, outer limit disk 52, interior limit Disk 53, outgassing groove 54, pressurization film.
Specific embodiment
The present invention will be further described with specific embodiment with reference to the accompanying drawing.
A method of intertidal zone aufwuch is sampled using portable sampler, is included the following steps:
A. a plastic film is covered on the rock surface positioned at intertidal zone, and is bonded in the edge seal of plastic film On rock, air gap is formed between plastic film and rock surface at this time.It should be noted that the preferred size of plastic film can In 1m × 1m between 2m × 2m, to facilitate the laying of plastic film, and the edge of plastic film can be by gluing accordingly Knot agent and rock be bonding or available quick hardening cement the is laid in edge of plastic film and the junction of rock.In addition, plastics Film is preferably EVA heat-shrinkable film.The Plastic film surface as made of EVA heat-shrinkable film is coarse and with higher Surface energy, thus be conducive to subsequent barnacle in the attachment of Plastic film surface.Especially since rock surface be not one can be with The plane of flattening, therefore, when plastic film is covered on rock surface, plastic film will form many folds.We can at this time Plastic film is heated with hair dryer or blowtorch etc., so that plastic film be made to be rapidly formed the pleat for shrinking and eliminating plastic film Wrinkle makes it become flat and endures.
B. vacuum plant connect on plastic film with the gas outlet of check valve, then start vacuum plant thus Extract the air between plastic film and rock surface out, plastic film at this time is close to rock surface, and plastic film is made to follow rock The concave-convex surface of stone rises and falls.
C. rock surface plastic covering film after a certain period of time, Plastic film surface have the barnacle young attachment, barnacle The young becoming the barnacle adult that is securely attached on plastic film after the metamorphosis in 7 stages, wherein preceding 6 stages For without the section stage, the last stage is gland Jie's stage.When Plastic film surface is attached with barnacle, by a sampling sleeve set The barnacle for firmly needing to sample, then make sample sleeve in formed vacuum, thus by suction by barnacle suck sampling sleeve in and Complete the sampling of barnacle.
In order to facilitate the sampling of sampling sleeve, as shown in Figure 1, the sampling upper end closed of sleeve 3, lower end are open-minded, sampling A constant pressure chamber 31 is arranged in the closed upper surface of sleeve, and several edge sampling sleeves are arranged on the lateral wall of sampling sleeve and axially prolong The hydraulic cylinder 32 stretched, hydraulic cylinder are arranged side by side on the lateral surface of sampling sleeve.In addition, being arranged in the inner cavity of hydraulic cylinder Moveable piston boit 33, taper bur 331 is arranged in the lower end that piston boit stretches out hydraulic cylinder, in the closed upper end of hydraulic cylinder Face is equipped with through hole 321, and the upper end of through hole penetrates through constant pressure chamber, the inner cavity of the lower end perforation hydraulic cylinder of through hole, hydraulic The top setting of inner cavity of cylinder compresses the positioning pressure spring 34 of piston boit, and intracavitary full of hydraulic oil in constant pressure, and hydraulic oil is by passing through Perforation enters the top of hydraulic cylinder inner cavity.On the one hand piston boit at this time can not be moved up close by the effect of positioning pressure spring Through hole, on the other hand, when piston boit moves down, the vacuum of constant pressure chamber that will cause hydraulic cylinder and penetrated through with it, therefore Piston boit also can not be moved down voluntarily.Therefore, each piston boit is in contour equilibrium state.
When sampling sleeve entangles the barnacle for needing to sample, since the rock surface around the barnacle position has height It is low, therefore, higher rock contact around the part conic bur elder generation and barnacle of the piston column end of all around stems is sampled, thus Piston boit is set to be pressurized and move up, it is intracavitary that the hydraulic oil of the hydraulic cylinder inner cavity where the piston boit enters constant pressure.Due at this time The taper bur of lower end is in the state that do not stress not in contact with the piston boit of rock, and therefore, the hydraulic oil into constant pressure chamber can be automatic Ground flows into piston boit and is in a hydraulic cylinder for the state that do not stress, so that actuator piston column moves down, makes each piston boit lower end Taper bur contradict rock simultaneously, and then realize each taper bur height and the height of rock corresponding points it is adaptive, bore Shape bur forms the continuous annular tearing hole of a circle on the plastic film of attachment barnacle;When sampling formation vacuum in sleeve, The plastic film for adhering to barnacle can be at tearing hole by tear fracture easily, so that barnacle be made to be inhaled into together with plastic film It samples in sleeve, complete barnacle can be obtained in the plastic film that sampling personnel need to only tear the small pieces being bonded together with barnacle off Sample sample.
In order to easily realize the vacuum of sampling sleeve, demarcation plate 35 can be arranged in sampling sleeve in we, and demarcation plate exists The vacuum pumping chamber on top and the accommodating cavity of lower part are separated out in sampling sleeve.Piston 36 is set in vacuum pumping chamber and is located at piston Following reseting pressuring spring 37, reseting pressuring spring lower end compress demarcation plate, and upper end compresses piston, and piston is set on the downside of demarcation plate The limited post 361 protruded into reseting pressuring spring is set, to play the role of positioning and guiding to reseting pressuring spring, avoids generating torsion when its compression Bent and skew.In addition, setting penetrates through the air inlet 351 of accommodating cavity and vacuum pumping chamber on demarcation plate, in the upper end of sampling sleeve The connector 38 of calming the anger of setting perforation vacuum pumping chamber, the connector of calming the anger are connected with portable inflator 4 on face.In addition, calming the anger Connector is also other to be connected to bleeder pipe 39, and angle valve 391 is arranged on bleeder pipe, to make bleeder pipe that can be rapidly closed or open-minded.
When needing to make to sample sleeve shaped into vacuum, sampling personnel first pass through pumping of the connector to sampling sleeve of calming the anger with inflator Air is gradually injected in vacuum chamber, gradually rises the air pressure in vacuum pumping chamber, and piston gradually overcomes the bullet of reseting pressuring spring at this time Power and move down, until the limited post of piston underside is positioned against demarcation plate.Certainly, angle valve at this time should be at closed state. Then, sampling personnel open rapidly angle valve, are connected to vacuum pumping chamber with ambient atmosphere by the bleeder pipe calmed the anger on connector, at this time Air pressure in vacuum pumping chamber declines rapidly, reseting pressuring spring then drive piston it is quick on move to against sampling upper cartridge closing End, so that parital vacuum is formed in the part moment in vacuum pumping chamber between piston and demarcation plate, the appearance below demarcation plate Set intracavitary air moment is entered in vacuum pumping chamber by the air inlet on demarcation plate, and accommodating cavity at this time forms the true of moment Sky, and then barnacle is sucked in the accommodating cavity for sampling sleeve lower end.
It is understood that sampling sleeve of the invention only forms the vacuum of a moment when extracting barnacle, and lead to Crossing inflator moves down piston gradually, be conducive to reduce sampling personnel working strength, in particular, we can be used it is existing Pedal tyre pump, thus can be further easy to operate and use.
For the ease of fast moving for piston, clearance fit is formed between piston and sampling sleeve, and in the circle of piston The sealed groove 362 of circular ring shape is set on circumferential surface, and the cross section of sealed groove is in rectangle, and setting has opening in sealed groove Steel sealing ring 363, the lateral surface of steel sealing ring, which reclines, samples the inner sidewall of sleeve.It is understood that steel seals The sealing effect of ring can be than the poor sealing effect of sealing ring made of rubber.Since piston of the invention is made in fast moving Accommodating cavity forms the vacuum of moment, and the air inlet of demarcation plate than the piston that is sealed by steel sealing ring with sample sleeve Between gap it is much bigger, therefore, in the moment that piston quickly moves up, the air in vacuum pumping chamber enters accommodating by piston The amount of chamber is minimum, will not influence the vacuum sample effect of sampling sleeve.
When installation, we can inwardly squeeze steel sealing ring, be fully sunk in steel sealing ring in sealed groove, to make Piston is convenient to be packed into sampling sleeve.Steel sealing ring struts outward by spontaneous elastic force at this time, to keep steel close The lateral surface of seal ring, which reclines, samples the inner sidewall of sleeve, realizes piston to the substantially sealed off of sampling sleeve.It is known that steel is close Coefficient of friction between seal ring and the sampling sleeve of steel will be much smaller than the coefficient of friction between rubber and steel, therefore, the present invention Piston can sampling sleeve in move rapidly.It should be noted that when piston connection steel sealing ring is packed into sealing sampling set When in cylinder, steel sealing ring struts outward and reclines and sample the inner sidewall of sleeve, and the opening of steel sealing ring should have at this time The installation gap of 1-1.5mm, in order to can inwardly squeeze steel sealing ring when piston is put into sampling sleeve.In addition, we are also The notch of a rectangle can be respectively set at two ends that steel sealing ring is open, thus the overlap joint dashed forward before open end forms one Item 364, two bonding jumpers shift to install to form complementation, and mutually stick together, to be conducive to improve in steel The sealing effect of the opening of sealing ring.
When we remove sampling barnacle together with plastic film, the plastic film being covered on rock will form one A hole, in order to avoid plastic film is mutually separated with rock after barnacle samples, we can also be further added by step d after step c: Adhesive layer first is set at the edge of a vacuum freshness retaining film, then vacuum freshness retaining film is covered on to rock exposed after barnacle samples Surface, the edge of the vacuum freshness retaining film bond on a plastic film;Then step b is repeated, plastic film and vacuum are protected Fresh film is close to rock surface together.
It should be noted that the size of vacuum freshness retaining film should be greater than the hole formed on a plastic film after barnacle sampling Size, and settable one layer of painting silicon protection sheet on the adhesive layer of vacuum freshness retaining film edge, to form adhesive sticker structure.Due to taking Shape, the size of hole being formed by plastic film after the sampling of sample sleeve be it is substantially stationary, therefore, we can be by vacuum The adhesive sticker paster of standard is made in preservative film, when needing coverage hole, need to only tear off and apply silicon protection sheet, can be by vacuum freshness retaining film It covers and is bonded at hole, certainly, EVA heat-shrinkable film substitution can also be used in vacuum freshness retaining film, to conveniently pass through heating It is set to shrink and entirely be covered on rock.
Finally, being arranged on the inner sidewall of connecting tube as shown in Fig. 2, connecting tube 11 can be arranged in we on plastic film 1 The inner hole of the limit plate 12 of intermediate porose circular ring shape, limit plate forms the gas outlet 121 on plastic film.Check valve includes Mobile column 5 in gas outlet is axially displaceably set, outside mobile column is integrally provided close to the outer end of connection tube opening Disk 51 is limited, the interior limit disk 52 of truncated cone-shaped is integrally provided in the inner end of mobile column, is arranged on the cylindrical surface of mobile column Axially through the outgassing groove 53 of interior limit disk, and vacuum plant can be a small-sized vacuum pump, have on vacuum pump Exhaust tube.
In this way, in stepb, the exhaust tube of vacuum plant can be first sealedly connected on the opening of connecting tube by we, so After start vacuum plant, the air pressure on the inside of limit plate at this time be greater than outside air pressure, mobile column is together with inside and outside limit disk in gas It is displaced outwardly under the action of pressure difference, interior limit disk is positioned against the inside of limit plate, outer limit disk and limit at this time Plate mutually separates, and the air between plastic film and rock is discharged by the outside that outgassing groove enters limit plate;Work as plastics When air between film and rock surface reduces to form vacuum, vacuum plant is closed, and remove the exhaust tube at connecting tube, this When limit plate on the inside of air pressure be less than the air pressure in outside, mobile column is inside under the action of draught head together with inside and outside limit disk Mobile, outer limit disk, to block venthole, makes to be continuously kept between plastic film and rock against the outside of limit plate Vacuum state, plastic film are close to rock surface.
Certainly, we can also limit the pressurization film 54 of circular cone annular made of the edge setting PVC film of disk outside, and A nut cap 13 is threadedly coupled in the opening of connecting tube.When the air between plastic film and rock surface reduces to form vacuum When, outer limit disk is close to the outside of limit plate, and outer limit disk blocks venthole against limit plate at this time, and circular cone is annular Pressurization film outer ledge be in close contact limit plate, thus to venthole formed dual-seal.And nut cap then can be to check valve Protection is formed, to make plastic film constantly be close to rock surface.

Claims (7)

1. a kind of method sampled using portable sampler to intertidal zone aufwuch, characterized in that include the following steps:
A. a plastic film is covered on the rock surface positioned at intertidal zone, and is bonded in the edge seal of plastic film On rock, air gap is formed between plastic film and rock surface at this time;
B. vacuum plant is connect with the gas outlet on plastic film with check valve, starts vacuum plant then to extract out Air between plastic film and rock surface, plastic film at this time are close to rock surface;
C. when Plastic film surface is attached with barnacle, a sampling sleeve is entangled into the barnacle for needing to sample, then makes to sample Vacuum is formed in sleeve, so that barnacle be sucked in sampling sleeve.
2. the method according to claim 1 sampled using portable sampler to intertidal zone aufwuch, characterized in that The upper end closed of the sampling sleeve, lower end are open-minded, constant pressure chamber are equipped in the upper surface of sampling sleeve, in the outside of sampling sleeve Wall is equipped with several hydraulic cylinders axially extending along sampling sleeve, and hydraulic cylinder is arranged side by side on the lateral surface of sampling sleeve, Piston boit is equipped in the hydraulic cylinder, the lower end that piston boit stretches out hydraulic cylinder is equipped with taper bur, closes in hydraulic cylinder Upper surface be equipped with through hole, the upper end of the through hole penetrates through constant pressure chamber, and the lower end of through hole penetrates through hydraulic cylinder, hydraulic The top of inner cavity of cylinder is equipped with the positioning pressure spring for compressing piston boit, and the constant pressure is intracavitary to be filled with hydraulic oil, when sampling sleeve set When the barnacle for firmly needing to sample, the taper bur for sampling the piston column end of all around stems is successively connect with the rock around barnacle Touching, so that the height of each taper bur and the height of rock corresponding points be made to be adapted, taper bur is thin in the plastics of attachment barnacle A continuous tearing hole of circle is formed on film;When sampling formation vacuum in sleeve, adhere to the plastic film of barnacle at tearing hole By tear fracture, so that barnacle be made to be inhaled into sampling sleeve together with plastic film.
3. the method according to claim 1 sampled using portable sampler to intertidal zone aufwuch, characterized in that The plastic film is EVA heat-shrinkable film.
4. the method according to claim 2 sampled using portable sampler to intertidal zone aufwuch, characterized in that It is equipped with demarcation plate in sampling sleeve, so that it is separated out the vacuum pumping chamber on top and the accommodating cavity of lower part in sampling sleeve, Piston and reseting pressuring spring are equipped in vacuum pumping chamber, the piston is equipped with the limit protruded into reseting pressuring spring in the downside close to demarcation plate Position column, the reseting pressuring spring lower end compress demarcation plate, and upper end compresses piston, and perforation accommodating cavity is equipped on demarcation plate and is vacuumized The air inlet of chamber, sampling sleeve upper surface on be equipped with perforation vacuum pumping chamber connector of calming the anger, it is described calm the anger connector with inflate Cylinder is connected, and the connector of calming the anger is also other to be connected to bleeder pipe, and angle valve is equipped on bleeder pipe, when inflator to vacuum pumping chamber gradually When injecting air, the elastic force of piston overpowers reseting pressuring spring and gradually move down, until limited post positions against demarcation plate;Work as opening When vacuum pumping chamber is communicated with the atmosphere in angle valve, reseting pressuring spring driving piston quickly moves up reset, to be located in vacuum pumping chamber Part moment between piston and demarcation plate forms parital vacuum, and then barnacle is sucked in the accommodating cavity for sampling sleeve lower end.
5. the method according to claim 4 sampled using portable sampler to intertidal zone aufwuch, characterized in that Clearance fit is formed between the piston and sampling sleeve, the periphery of piston is equipped with the sealed groove of circular ring shape, is sealing The steel sealing ring with opening is equipped in groove, two ends of the steel sealing ring opening are respectively equipped with bonding jumper, two ends Bonding jumper mutual dislocation, the lateral surface of steel sealing ring, which reclines, samples the inner sidewall of sleeve.
6. the method according to claim 1 sampled using portable sampler to intertidal zone aufwuch, characterized in that It is further added by step d after step c: adhesive layer being set at the edge of a vacuum freshness retaining film, then covers the vacuum freshness retaining film Exposed rock surface after barnacle sampling is covered, the edge of the vacuum freshness retaining film is bonded on a plastic film, then repeated Step b, makes plastic film be close to rock surface again.
7. the method according to claim 4 sampled using portable sampler to intertidal zone aufwuch, characterized in that It is equipped with connecting tube on a plastic film, the limit plate of circular ring shape, the inner hole of the limit plate are equipped on the inner sidewall of connecting tube The gas outlet is formed, the check valve includes the mobile column being arranged in gas outlet, is opened in mobile column close to connecting tube The outer end of mouth is integrally provided with outer limit disk, in the inner end of mobile column integrally provided with the interior limit disk of truncated cone-shaped, outside The edge for limiting disk is equipped with the pressurization film of circular cone annular made of PVC film, and the cylindrical surface of the mobile column is equipped with along axis To the outgassing groove through interior limit disk, it is threaded with nut cap in the opening of connecting tube, in stepb, first by vacuum holding The exhaust tube set is sealedly connected on the opening of connecting tube, then starts vacuum plant, and the air pressure on the inside of limit plate is greater than at this time The air pressure in outside, mobile column are displaced outwardly together with inside and outside limit disk, and for interior limit disk against the inside of limit plate, plastics are thin Air between film and rock is discharged by gas outlet;When forming vacuum between plastic film and rock surface, close Vacuum plant, and remove the exhaust tube at connecting tube, the at this time air pressure on the inside of limit plate are less than the air pressure in outside, mobile column together with Inside and outside limit disk moves inward, and it is then close with the lateral surface of limit plate to be pressurized film against the outside of limit plate for outer limit disk Fitting finally covers nut cap again, plastic film can be made constantly to be close to rock table to form dual-seal to gas outlet Face.
CN201810615042.4A 2018-06-14 2018-06-14 Method for sampling intertidal zone attached organisms by using portable sampling device Active CN109006723B (en)

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CN112471089A (en) * 2020-11-25 2021-03-12 董献革 Auxiliary capturing device for mantis shrimps at beach mouth

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CN112471089A (en) * 2020-11-25 2021-03-12 董献革 Auxiliary capturing device for mantis shrimps at beach mouth
CN112471089B (en) * 2020-11-25 2022-11-15 滨州市胜英水产有限公司 Auxiliary capturing device for mantis shrimps at beach mouth

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