CN109006352A - A kind of bletilla seed sowing bioactivity seedling medium configuration method - Google Patents

A kind of bletilla seed sowing bioactivity seedling medium configuration method Download PDF

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Publication number
CN109006352A
CN109006352A CN201811080080.0A CN201811080080A CN109006352A CN 109006352 A CN109006352 A CN 109006352A CN 201811080080 A CN201811080080 A CN 201811080080A CN 109006352 A CN109006352 A CN 109006352A
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China
Prior art keywords
matrix
bletilla
added
bioactivity
thick
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Pending
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CN201811080080.0A
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Chinese (zh)
Inventor
陈燚格
梁琼
王杰
段锦成
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Wuhan Botanical Garden of CAS
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Wuhan Botanical Garden of CAS
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Priority to CN201811080080.0A priority Critical patent/CN109006352A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/30Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds

Abstract

The invention discloses a kind of configuration methods of bletilla seed sowing bioactivity seedling medium, are related to bletilla cultivation technique.This method mainly includes kind of bed and two layers of seed layer: 1. plant bed substrate: 3~10cm is thick, river sand: carbendazim=1000:5, after mixing, solar exposure 10~15 days;2. seed layer matrix: 2~5cm is thick, and concrete configuration process is as follows: A, matrix prepares;B, matrix sterilizes;C, microbial bacterial agent and sieving is added.Compared with prior art, the present invention has following advantages and good effect: 1. Diammonium phosphate (DAP) and bacillus megaterium is added in the present invention in matrix, makes matrix enhancement bioactivity, improves bletilla seed sowing germination rate;2. the present invention carries out matrix sterilizing using cauldron cooking process, facilitate worker's practical operation, while also reducing job costs.

Description

A kind of bletilla seed sowing bioactivity seedling medium configuration method
Technical field
The present invention relates to bletilla cultivation technique more particularly to a kind of configurations of bletilla seed sowing bioactivity seedling medium Method.
Background technique
Bletilla[Bletilla striata(Thunb.ex A.Murray)Rchb.f.]For orchid family bletilla category perennial grass This plant, the high 18-60cm of plant;Pseudobulb oblate spheroid, annulus as upper mask water chestnut, rich viscosity;Stem thickness is strong, rigidness.
Bletilla Chang Yesheng covers thick grass or hayashishita wetland in hills, low mountain stream side and yin, is distributed on the south China Qinling Mountains The ground such as provinces and regions and Japan, are used as medicine with stem tuber, have tonifying lung hemostasis, detumescence and promoting granulation and other effects;Simultaneously be also widely used in medicine, The industries such as beauty, coating, printing and dyeing.In recent years, since demand is big, for wild bletilla by serious private digging coyoting, stock number is anxious It reduces sharply few, has been included in world endangered plant protection kind, and by " Chinese Plants Red Data Book-rare extinction plants " the 1st It includes, while being also written into " endangered species of wild fauna and flora international trade pact " (CITES) protected species.
Currently, the main modes of reproduction of bletilla is the division propagation and tissue cultures by pseudobulb, higher cost.It forms Ripe bletilla seed is very tiny, and suspensor degenerates and without endosperm, and seed germination rate is low in its natural state.Therefore, it greatly develops Bletilla seed sowing sexual propagation is bletilla industrialization effective way and inexorable trend.A kind of life suitable for bletilla seed sowing Object active matrix can not only improve bletilla seed sowing germination rate, provide the foundation for the quick sexual propagation of bletilla, while because of it Preparation effectively specification, also provides a kind of reference for bletilla industrialized development.
ZL201710193878.5 discloses a kind of bletilla striata seeds direct sowing and seedling matrix and preparation method thereof, is largely divided into kind Bed and two layers of seed layer.Wherein:
Kind of bed proportion be mountain sand: decomposed organic matter: decay sheep excrement: ternary compound fertilizer: zinc sulfate for 80~100:8~30:3~ 10:1~4:0.1~1;
Seed layer proportion is mountain sand: decomposed organic matter: ternary compound fertilizer: zinc sulfate: carbendazim: potassium dihydrogen phosphate: ammonium molybdate is 30~60:30~60:1~4:0.1~1:0.1~1.5:0.1~1:0.1~1.
ZL201711312284.8 discloses a kind of configuration method of Dabie Mountain wild bletilla striata nutrition sowing media, and main point At two layers, wherein upper thickness is 2cm, lower thickness 4cm.Overlayer matrix proportion is turf: active carbon volume ratio is 50:1; Underlying substrate is the bark powder fermented: turf: chicken manure: vermiculite volume ratio 3:4:1:2, then carries out matrix sterilizing, uses first Pressure cooker sterilizing 30min, 121 DEG C, is then puddled with carbendazim, nutrient matrix and carbendazim volume ratio 1000:5.
ZL201610587999.3 discloses a kind of bletilla plantation live streaming mating system, and wherein substrate composition is coco bran: rotten Grow soil: the mixing of organic substrate volume ratio 1:1:2 ratio is added the abundant decomposed cow dung of said mixture total weight 10%, turns over repeatedly It is dynamic to mix them thoroughly, culture substrate is irrigated with 1000 times of 50% carbendazim, is put under sunlight and is exposed to the sun 10~15 days, until culture medium After qualitative change is dry, cross the sieve of hole 0.6mm to obtain the final product.
Summary of the invention
The object of the invention is that providing a kind of configuration method of bletilla seed sowing bioactivity seedling medium, not only Bletilla seed sowing germination rate is improved, is provided the foundation for the quick sexual propagation of bletilla, while because it prepares effectively specification, A kind of reference is provided for bletilla industrialized development.
This method mainly includes kind of bed and two layers of seed layer.
1. planting bed substrate: 3~10cm is thick, river sand: carbendazim=1000:5, after mixing, solar exposure 10~15 It;
2. seed layer matrix: 2~5cm is thick, and concrete configuration process is as follows:
A, matrix prepares: by coconut shell flour: loess: northeast turfy soil=8~15:3~5:15~20, after being sufficiently mixed, according to often standing 2kg chicken manure is added in square rice, is sufficiently stirred again;
B, matrix sterilizes: matrix is put into rural area cauldron, adds water that just to surface is flooded, after boiling is dry, matrix is turned over one time, It adds water to again and floods the water surface, after boiling is dry, matrix is divided in cement flooring;By every cubic metre plus 2kg after matrix is cooling It is stirred and evenly mixed after phosphoric acid diamines;
C, microbial bacterial agent and sieving is added: bacillus megaterium is added according to weight ratio 1000:1 in the matrix after sterilizing, is filled Point mix after cross aperture be 6mm sieve to obtain the final product.
Compared with existing bletilla seedling medium, the working mechanism and innovative point of this method are:
Compared with existing bletilla seed sowing seedling medium, this method is not only the mixing such as coconut shell flour, Nutrition Soil, but is being gone out Diammonium phosphate (DAP) and bacillus megaterium are added after bacterium, so that matrix has bioactivity, improves the sprouting of bletilla seed sowing Rate;Meanwhile the sterilizing of this matrix adds water boiling using cauldron, facilitates worker's practical operation, reduces job costs.
Compared with prior art, the present invention has following advantages and good effect:
1. Diammonium phosphate (DAP) and bacillus megaterium is added in the present invention in matrix, makes matrix enhancement bioactivity, improve white And seed sowing germination rate;
2. the present invention carries out matrix sterilizing using cauldron cooking process, facilitate worker's practical operation, while also reducing job costs.
Detailed description of the invention
Fig. 1 is boiling cauldron picture;
Fig. 2 is matrix cleaning sieving picture;
Fig. 3 is the stand-by picture of medium in tray;
Fig. 4 is the substantially all greening picture of bletilla seed;
Fig. 5 is that bletilla seed shows true leaf picture.
Specific embodiment
It is described in detail with reference to the accompanying drawings and examples:
Such as Fig. 1,2,3,4,5.
One, embodiment 1
1, bed substrate is planted:
River sand: carbendazim 1000:5, after mixing solar exposure 10-15 days.
2, seed layer matrix:
1) matrix prepares: by coconut shell flour: loess: northeast turfy soil is 8:3:15, after being sufficiently mixed, is added according to every cubic metre 2kg chicken manure, is sufficiently stirred again;
2) matrix sterilizes: matrix being put into rural area cauldron first, adds water that just to surface is flooded, after boiling is dry, matrix is turned over It one time, adds water to flood the water surface again, after boiling is dry, matrix is divided in cement flooring;Every cubic metre is pressed after matrix is cooling It is stirred and evenly mixed after adding 2kg phosphoric acid diamines;
3) microbial bacterial agent and sieving is added: bacillus megaterium is added according to weight ratio 1000:1 in the matrix after sterilizing, is filled Point mix after cross aperture be 6mm sieve to obtain the final product.
3, kind of a bed substrate is spread in flat plate, with a thickness of 3cm, hugged with bamboo chip it is flat, then by ready seed layer In matrix tiling to kind of bed, with a thickness of 2cm, hugged with bamboo chip flat;
4, the bletilla seed handled well is sowed on seedlings nursing plate.
After 10 days, bletilla seed is substantially all existing green, and germination rate 90-98% goes out true leaf in 20-25 days.
Two, embodiment 2
1, bed substrate is planted:
River sand: carbendazim 1000:5, after mixing solar exposure 10-15 days;
2, seed layer matrix:
1) matrix prepares: by coconut shell flour: loess: northeast turfy soil is 15:4:15, after being sufficiently mixed, is added according to every cubic metre 2kg chicken manure, is sufficiently stirred again;
2) matrix sterilizes: matrix being put into rural area cauldron first, adds water that just to surface is flooded, after boiling is dry, matrix is turned over It one time, adds water to flood the water surface again, after boiling is dry, matrix is divided in cement flooring, it is cooling to matrix, by every cubic metre It is stirred and evenly mixed after adding 2kg phosphoric acid diamines;
3) microbial bacterial agent and sieving is added: bacillus megaterium is added according to weight ratio 1000:1 in the matrix after sterilizing, is filled Divide after mixing the sieve for crossing that aperture is 6mm;
3, kind of a bed substrate is spread in flat plate, with a thickness of 3cm, is hugged with bamboo chip flat: again by ready seed layer matrix It tiles onto kind of bed, with a thickness of 2cm, is hugged with bamboo chip flat;
4, the bletilla seed handled well is sowed on seedlings nursing plate:
After 10 days, bletilla seed is substantially all existing green, and germination rate 95-98% goes out true leaf in 20-25 days.

Claims (1)

1. a kind of configuration method of bletilla seed sowing bioactivity seedling medium, it is characterised in that:
1. planting bed substrate: 3~10cm is thick, river sand: carbendazim=1000:5, after mixing, solar exposure 10~15 days;
2. seed layer matrix: 2~5cm is thick, and concrete configuration process is as follows:
A, matrix prepares: by coconut shell flour: loess: northeast turfy soil=8~15:3~5:15~20, after being sufficiently mixed, according to often standing 2kg chicken manure is added in square rice, is sufficiently stirred again;
B, matrix sterilizes: matrix is put into rural area cauldron, adds water that just to surface is flooded, after boiling is dry, matrix is turned over one time, It adds water to again and floods the water surface, after boiling is dry, matrix is divided in cement flooring;By every cubic metre plus 2kg after matrix is cooling It is stirred and evenly mixed after phosphoric acid diamines;
C, microbial bacterial agent and sieving is added: bacillus megaterium is added according to weight ratio 1000:1 in the matrix after sterilizing, is filled Point mix after cross aperture be 6mm sieve to obtain the final product.
CN201811080080.0A 2018-09-17 2018-09-17 A kind of bletilla seed sowing bioactivity seedling medium configuration method Pending CN109006352A (en)

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CN201811080080.0A CN109006352A (en) 2018-09-17 2018-09-17 A kind of bletilla seed sowing bioactivity seedling medium configuration method

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109452127A (en) * 2019-01-07 2019-03-12 云南省农业科学院热带亚热带经济作物研究所 It is a kind of improve Fructus Aurantii emergence rate seedling medium and its application
CN109496816A (en) * 2019-01-07 2019-03-22 云南省农业科学院热带亚热带经济作物研究所 A kind of method for culturing seedlings improving Fructus Aurantii emergence rate
CN109496816B (en) * 2019-01-07 2021-06-01 云南省农业科学院热带亚热带经济作物研究所 Seedling raising method for improving emergence rate of fructus aurantii

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05148115A (en) * 1991-07-08 1993-06-15 Sanko Kk Simple process for sowing, sprouting and transplantation of orchid
CN104996138A (en) * 2015-07-07 2015-10-28 贵州国成生态种苗场 Method for shortening seedling raising time limit of uncaria rhynchophylla seeds
CN105110825A (en) * 2015-07-23 2015-12-02 云南省烟草公司普洱市公司 Organic light seedling medium, and preparation method thereof
CN107056434A (en) * 2017-03-28 2017-08-18 云南恩润生物科技发展有限公司 Bletilla striata seeds direct sowing and seedling matrix and preparation method thereof
CN108029490A (en) * 2018-01-19 2018-05-15 遵义琦琅生物技术咨询有限公司 A kind of bletilla striata seeds direct sowing and seedling matrix and its method for culturing seedlings

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05148115A (en) * 1991-07-08 1993-06-15 Sanko Kk Simple process for sowing, sprouting and transplantation of orchid
CN104996138A (en) * 2015-07-07 2015-10-28 贵州国成生态种苗场 Method for shortening seedling raising time limit of uncaria rhynchophylla seeds
CN105110825A (en) * 2015-07-23 2015-12-02 云南省烟草公司普洱市公司 Organic light seedling medium, and preparation method thereof
CN107056434A (en) * 2017-03-28 2017-08-18 云南恩润生物科技发展有限公司 Bletilla striata seeds direct sowing and seedling matrix and preparation method thereof
CN108029490A (en) * 2018-01-19 2018-05-15 遵义琦琅生物技术咨询有限公司 A kind of bletilla striata seeds direct sowing and seedling matrix and its method for culturing seedlings

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109452127A (en) * 2019-01-07 2019-03-12 云南省农业科学院热带亚热带经济作物研究所 It is a kind of improve Fructus Aurantii emergence rate seedling medium and its application
CN109496816A (en) * 2019-01-07 2019-03-22 云南省农业科学院热带亚热带经济作物研究所 A kind of method for culturing seedlings improving Fructus Aurantii emergence rate
CN109496816B (en) * 2019-01-07 2021-06-01 云南省农业科学院热带亚热带经济作物研究所 Seedling raising method for improving emergence rate of fructus aurantii

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