CN108865649B - Culture sampler for marine bacteria - Google Patents

Culture sampler for marine bacteria Download PDF

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Publication number
CN108865649B
CN108865649B CN201810433179.8A CN201810433179A CN108865649B CN 108865649 B CN108865649 B CN 108865649B CN 201810433179 A CN201810433179 A CN 201810433179A CN 108865649 B CN108865649 B CN 108865649B
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incubator
cylinder
chamber
sampler
temperature
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CN201810433179.8A
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CN108865649A (en
Inventor
张莉
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Qingdao Agricultural University
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Qingdao Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/04Phase separators; Separation of non fermentable material; Fractionation

Abstract

The present invention relates to the culture samplers for marine bacteria.It includes incubator, the agitating device for simulated flow flowing, sampler and the control device for controlling culture the temperature inside the box, the agitating device is set at the top of incubator, the control device is set in culture box inner wall, the incubator bottom is formed with support, and the sampler is threadedly connected to incubator bottom.The true water environment of marine bacteria existence is simulated by agitating device, the water sample containing marine bacteria in incubator is sampled by sampler, in sampling process, marine bacteria is effectively avoided by sampler and is adhered on the lateral wall of utensil, certain pollution is caused;The temperature in incubator is controlled by control device, requirement when meeting Bacteria Culture to temperature.

Description

Culture sampler for marine bacteria
Technical field
The present invention relates to the culture samplers for marine bacteria.
Background technique
When marine bacteria is sampled culture, Bacteria Culture work can be generated due to containing more impurity in water sampling Certain interference, such as solid impurity, fiber, water plant, algae and heavy metal etc., it is assumed that the water sample comprising above-mentioned object directly into It if row marine bacteria is cultivated, can not only result in blockage to culture apparatus, increase cleaning difficulty, it is also quite conceivable to influence culture effect Fruit increases culture difficulty.
Summary of the invention
Present invention seek to address that the above problem, provides the culture sampler for marine bacteria, pass through stirring dress Set simulation marine bacteria existence true water environment, by sampler to the water sample containing marine bacteria in incubator into Row sampling, in sampling process, marine bacteria is effectively avoided by sampler and is adhered on the lateral wall of utensil, is caused Certain pollution;The temperature in incubator is controlled by control device, requirement when meeting Bacteria Culture to temperature passes through filtering Case is filtered water plant, algae and few fibers in advance, and by fraction solids ocean contamination precipitation, is made by the way that device is precipitated Heavy metal is arranged on precipitating inside pipe wall different location by type difference, and the coverage values of deposition are directly displayed by display screen, Facilitating user has one adequately to understand water sample;The technical scheme adopted is as follows:
For the culture sampler of marine bacteria, including incubator, the agitating device for simulated flow flowing, sampling Device and control device for controlling culture the temperature inside the box, the agitating device are set at the top of incubator, and the control fills It installs and is placed in culture box inner wall, the incubator bottom is formed with support, and the sampler is threadedly connected to incubator Bottom.
Based on the above technical solution, the agitating device includes bottom plate, mounting plate, turntable, motor and agitator arm, The bottom plate unloading is connected at the top of incubator, and the mounting plate is fixed on bottom plate vertically, and the turntable is set and the company of rotation It is connected to mounting plate side, the motor is connected with turntable, and is set to the other side of mounting plate;It is fixed to be equipped at the top of the mounting plate Position block, the locating piece are rotationally connected on mounting plate, and are located at the surface of turntable;The agitator arm is threaded onto locating piece In, and be fixed on turntable by sliding block, and agitator arm and sliding block are rotatablely connected.
Based on the above technical solution, the sampler includes shell, sealing ring, connecting rod, the first spring and shelves Bar, the shell is threadedly connected to incubator bottom, and is connected with incubator;Lateral partition is equipped in the shell, Enclosure interior cavity is divided into upper plenum and lower cavity by partition, and the partition is equipped with for being connected to the logical of upper plenum and lower cavity Hole, the connecting rod are threaded onto partition, and the sealing ring is fixed at the top of connecting rod, and sealing ring overlapping edges are in case top; The sealing ring is less than or equal to the inner surface of incubator bottom, and first spring pocket is loaded on connecting rod, and is located at lower cavity It is interior;The lever is fixed in connecting rod end, and the height under the lever free state is higher than the bottom surface of support.
Based on the above technical solution, the control device includes cylinder body, temperature-sensitive module, cylinder and reset spring, The cylinder body is fixed on the inner sidewall of incubator and is located at the gas vent of incubator, be equipped in the cylinder body first chamber, Second chamber, the first release channel and the second release channel, first release channel and the second release channel with the first chamber Room is connected, and one end of the first chamber of the cylinder body is provided with temperature-sensitive module, and the other end is connected with second chamber;Described Two chambers are connected with the gas vent on incubator box body, and the temperature-sensitive module includes temperature susceptibility contact terminal, temperature-sensitive shell and diaphragm, described Temperature-sensitive shell is fixed on the port of cylinder body first chamber, and diaphragm is set in temperature-sensitive shell, and outside is connected with temperature susceptibility contact terminal;The diaphragm and column Body one end is connected, and the cylinder is threaded onto the first chamber and second chamber of cylinder body, and the other end of cylinder forms bulge, The bulge is located in second chamber, and the port diameter of second chamber is greater than the port diameter of first chamber, the reset Spring is located in second chamber, and its both ends is inconsistent with the inner sidewall of the bulge of cylinder and cylinder body respectively.
Based on the above technical solution, further include baffle, be connected by rod piece with the bulge of cylinder, and rod piece It is threaded onto reset spring;The baffle is located at the gas vent of incubator, and has a diameter larger than the diameter of gas vent.
It based on the above technical solution, further include Rose Box for impurity screening, the Rose Box and incubator It is connected, the Rose Box includes end cap, storage box and support tube, and the end cap is detachably connected with storage box, and on end cap Equipped with inlet and outlet;The inlet and outlet is connected with storage box respectively, and import is connected with extraneous initial water sample, out Mouth is connected with incubator;The venthole for filtering is formed on the support tube side wall, the support tube is located at storage box Interior and be connected with end cap by connector, the internal cavities of the support tube are connected with outlet.
Based on the above technical solution, copper wire is wound on the support tube lateral wall.
Based on the above technical solution, the rotation center of circle of the locating piece and the center of circle of turntable are in same straight line On.
Based on the above technical solution, at least provided with upper and lower two arc shaped hooks on the agitator arm.
It based on the above technical solution, further include precipitation device for heavy metal to be precipitated, the precipitation device packet Include sediment tube, magnet, lamp, optical fiber, photosensitive probe, electronic circuit and display screen, the sediment tube be set to Rose Box with In the pipeline that incubator is connected, the sediment tube is located on magnet, and described optical fiber one end is connect with lamp, and light is led to On the measuring point of sediment tube setting, and the optical signal across sediment tube is received by photosensitive probe, turned through electronic circuit amplification, A/D Processing is changed, shows corresponding numerical value on a display screen.
The present invention has the advantage that the true water environment of marine bacteria existence is simulated by agitating device, by taking Sampling device is sampled the water sample containing marine bacteria in incubator, effective by sampler in sampling process It avoids marine bacteria to be adhered on the lateral wall of utensil, causes certain pollution;It is controlled in incubator by control device Temperature, requirement when meeting Bacteria Culture to temperature are filtered water plant, algae and few fibers by Rose Box in advance, And by fraction solids ocean contamination precipitation, heavy metal is made to be arranged in precipitating inside pipe wall difference by type difference by the way that device is precipitated On position, and the coverage values of deposition are directly displayed by display screen, facilitating user has one adequately to understand water sample;It is whole A culture apparatus is reasonable in design, easy to use, practical.
Detailed description of the invention
Fig. 1: structural schematic diagram of the invention;
Fig. 2: the structural schematic diagram of control device of the present invention;
Fig. 3: the structural schematic diagram of sampler of the present invention.
Specific embodiment
Present invention will be further explained below with reference to the attached drawings and examples:
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected;It can To be mechanical connection, it is also possible to be electrically connected;It can be directly connected, it can also be indirectly connected through an intermediary.For this For the those of ordinary skill in field, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
As shown in Figure 1 to Figure 3, the culture sampler for marine bacteria of the present embodiment, including incubator 1, be used for Agitating device 2, sampler 3 and the control device 4 for controlling culture the temperature inside the box of simulated flow flowing, the stirring dress It sets 2 and is set to 1 top of incubator, the control device 4 is set on 1 inner sidewall of incubator, and 1 bottom of incubator is formed with Support, the sampler 3 are threadedly connected to 1 bottom of incubator.
Preferably, be set forth below a kind of specific agitating device, the agitating device 2 include bottom plate 2-1, mounting plate 2-2, Turntable 2-3, motor and agitator arm 2-5, the bottom plate 2-1 unloading are connected to 1 top of incubator, and the mounting plate 2-2 is solid vertically Be connected on bottom plate 2-1, the turntable 2-3 sets and is rotationally connected with the side mounting plate 2-2, the motor (not shown) with Turntable 2-3 is connected, and is set to the other side of mounting plate 2-2;Locating piece 2-6, the positioning are equipped at the top of the mounting plate 2-2 Block 2-6 is rotationally connected on mounting plate 2-2, and is located at the surface of turntable 2-3;The agitator arm 2-5 is threaded onto locating piece 2-6 In, and be fixed on turntable 2-3 by sliding block 2-7, and agitator arm 2-5 and sliding block 2-7 is rotatablely connected.
Preferably, be set forth below a kind of specific sampler, the sampler 3 include shell 3-1, sealing ring 3-2, Connecting rod 3-3, the first spring 3-4 and lever 3-5, the shell 3-1 is threadedly connected to 1 bottom of incubator, and is connected with incubator 1 It is logical;Lateral partition 3-6 is equipped in the shell 3-1, shell 3-1 internal cavities are divided into upper plenum 3-11 and lower sky by partition Chamber 3-12, the partition 3-6 are equipped with the through-hole 3-61 for being connected to upper plenum and lower cavity, the connecting rod 3-3 be threaded onto every In plate 3-6, the sealing ring 3-2 is fixed at the top of connecting rod 3-3, and sealing ring 3-2 overlapping edges are at the top of shell 3-1;It is described Sealing ring 3-2 is less than or equal to the inner surface of 1 bottom of incubator, and the first spring 3-4 is set on connecting rod 3-3, and is located at In lower cavity 3-12;The lever 3-5 is fixed in the end connecting rod 3-3, and the height under the lever 3-5 free state is higher than support Bottom surface.
Lever is resisted by sampling bottle or probe tube, and lever is regular upwards, so that sealing ring is separated with shell, sample Liquid is flowed downwardly into sampling bottle or probe tube by through-hole, and after sampling, sampling bottle or probe tube are separated with lever, Sealing ring is fallen after rise under the action of the first spring to the top of shell, and sample liquid is stopped to continue to flow out, convenient and efficient.
By rotating drainpipe, in cylinder and blocking portion it is inconsistent, and blocking portion is jacked up upwards, so that close Seal is separated with inner cylinder, and waste liquid is flowed downwardly into drainpipe by through-hole, reversely rotates drainpipe, cylinder and stop portions From sealing ring is fallen after rise under the action of the spring to the top of inner cylinder, and waste liquid is stopped to continue to flow out, and thus controls releasing for waste liquid It puts, it is convenient and efficient.
Preferably, a kind of specific control device is set forth below, the control device 4 includes cylinder body 4-1, temperature-sensitive module 4- 2, cylinder 4-3 and reset spring 4-4, the cylinder body 4-1 are fixed in the gas vent on the inner sidewall of incubator 1 and being located at incubator It is equipped with first chamber 4-11, second chamber 4-12, the first release channel 4-13 and second at 1-1, in the cylinder body 4-1 and discharges and leads to Road 4-14, the first release channel 4-13 and the second release channel 4-14 are connected with first chamber 4-11, the cylinder body One end of the first chamber 4-11 of 4-1 is provided with temperature-sensitive module 4-2, and the other end is connected with second chamber 4-12;Described second Chamber 4-12 is connected with the gas vent 1-1 on incubator box body, and the temperature-sensitive module 4-2 includes temperature susceptibility contact terminal 4-21, temperature-sensitive Shell 4-22 and diaphragm 4-23, the temperature-sensitive shell 4-22 are fixed on the port of cylinder body 4-1 first chamber 4-11, set in temperature-sensitive shell 4-22 Diaphragm 4-23, outside are connected with temperature susceptibility contact terminal 4-21;The diaphragm 4-23 is connected with the one end cylinder 4-3, and the cylinder 4-3 is installed In the first chamber 4-11 and second chamber 4-12 of cylinder body 4-1, and the other end of cylinder 4-3 forms bulge 4-31, described Bulge 4-31 is located in second chamber 4-12, and the port diameter of second chamber 4-12 is straight greater than the port of first chamber 4-11 Diameter, the reset spring 4-4 are located in second chamber 4-12, and its both ends respectively with the bulge 4-31 and cylinder body of cylinder 4-3 The inner sidewall of 4-1 is inconsistent.
The temperature in incubator is detected by temperature susceptibility contact terminal, when temperature reaches certain value, temperature susceptibility contact terminal transmits temperature To temperature-sensitive shell, temperature-sensitive shell expanded by heating is stretched, exceptionally straight so as to cause diaphragm stretching, and then drives gas vent of the cylinder to incubator Side is mobile, and cylinder is connected with baffle, when cylinder is mobile, drives baffle mobile, the unobstructed release of the gas vent of incubator at this time, High-temperature gas in incubator is discharged by gas vent to the external world, and when temperature is when within preset value, temperature-sensitive shell is shunk, and diaphragm is received Contracting restores to the original state, and by the double action with reset spring, drives cylinder mobile, baffle restores initial position, the row of incubator Stomata is blocked.
Preferably, further include baffle 4-5, be connected by rod piece 4-51 with the bulge 4-31 of cylinder 4-3, and rod piece 4- 51 are threaded onto reset spring 4-4;The baffle 4-5 is located at the gas vent 1-1 of incubator 1, and has a diameter larger than gas vent The diameter of 1-1.
It preferably, further include Rose Box 5 for impurity screening, the Rose Box 5 is connected with incubator 1, the mistake Filter box 5 includes end cap 5-1, storage box 5-2 and support tube 5-3, and the end cap 5-1 and storage box 5-2 is detachably connected, and end cap 5-1 is equipped with inlet and outlet;The inlet and outlet is connected with storage box 5-2 respectively, and import and extraneous initial water sample It is connected, outlet is connected with incubator;The venthole 5-31 for filtering is formed on the support tube 5-3 side wall, it is described Support tube 5-3 is located in storage box 5-2 and is connected by connector with end cap 5-1, the internal cavities of the support tube 5-3 and out Mouth is connected.
Preferably, it is wound with copper wire on the support tube 5-3 lateral wall, the tightness by adjusting copper wire winding controlled Filter gap.
Preferably, the rotation center of circle of the locating piece 2-6 and the center of circle of turntable 2-3 are on same straight line.
Further, on the agitator arm 2-5 at least provided with upper and lower two arc shaped hook 2-51, by multi-level arc shaped hook, The water flow mobility status that ocean different depth waters can be simulated, so that culture environment is truer.
Further, further include precipitation device 6 for heavy metal to be precipitated, the precipitation device 6 include sediment tube 6-1, Magnet 6-2, lamp 6-3, optical fiber 6-4, photosensitive probe 6-5, electronic circuit 6-6 and display screen 6-7, the sediment tube 6-1 are set It is placed in the pipeline that Rose Box 3 is connected with incubator 1, the sediment tube 6-1 is located on magnet 6-2, the optical fiber 6-4 One end is connect with lamp 6-3, light is led on the measuring point of sediment tube 6-1 setting, and is received by photosensitive probe 6-5 across precipitating The optical signal of pipe shows corresponding numerical value through electronic circuit 6-6 amplification, A/D conversion process on display screen 6-7.
It is offered on the bottom plate for the movable through slot of agitator arm, it is close that the through slot is equipped with the malleable elasticity of tool Sealer (not shown), the agitator arm are threaded onto sealing film, and seal film with agitator arm activity.
It is rotated by motor driven turntable, turntable drives agitator arm to rotate by sliding block, and agitator arm is threaded onto due to one end In locating piece, so agitator arm becomes straight reciprocating motion from rotary motion, so that the arc shaped hook of agitator arm is pressed in incubator It is moved according to certain track, to imitate water flow flowing.
The true water environment that marine bacteria existence is simulated by agitating device, by sampler to containing in incubator There is the water sample of marine bacteria to be sampled, in sampling process, marine bacteria is effectively avoided by sampler and is adhered to On the lateral wall of utensil, certain pollution is caused;The temperature in incubator is controlled by control device, when meeting Bacteria Culture pair The requirement of temperature is filtered water plant, algae and few fibers in advance by Rose Box, and fraction solids ocean impurity is sunk It forms sediment, by the way that device is precipitated heavy metal is arranged on precipitating inside pipe wall different location by type difference, and straight by display screen The coverage values for connecing display deposition, facilitating user has one adequately to understand water sample.
The present invention is described by way of example above, but the present invention is not limited to above-mentioned specific embodiment, all to be based on Any changes or modifications that the present invention is done are fallen within the scope of the claimed invention.

Claims (8)

1. being used for the culture sampler of marine bacteria, which is characterized in that including incubator (1), for simulated flow flowing Agitating device (2), sampler (3) and the control device (4) for controlling culture the temperature inside the box, the agitating device (2) set It is placed at the top of incubator (1), the control device (4) is set on incubator (1) inner sidewall, incubator (1) bottom shape At there is a support, the sampler (3) is threadedly connected to incubator (1) bottom, the agitating device (2) include bottom plate (2-1), Mounting plate (2-2), turntable (2-3), motor and agitator arm (2-5), bottom plate (2-1) unloading are connected at the top of incubator (1), The mounting plate (2-2) is fixed in vertically on bottom plate (2-1), and the turntable (2-3) is set and is rotationally connected with mounting plate (2-2) Side, the motor are connected with turntable (2-3), and are set to the other side of mounting plate (2-2);At the top of the mounting plate (2-2) Equipped with locating piece (2-6), the locating piece (2-6) is rotationally connected on mounting plate (2-2), and positioned at turntable (2-3) just on Side;The agitator arm (2-5) is threaded onto locating piece (2-6), and is fixed on turntable (2-3) by sliding block (2-7), and stirs Bar (2-5) and sliding block (2-7) are rotatablely connected.
2. the culture sampler according to claim 1 for marine bacteria, it is characterised in that: the sampler It (3) include shell (3-1), sealing ring (3-2), connecting rod (3-3), the first spring (3-4) and lever (3-5), the shell (3-1) It is threadedly connected to incubator (1) bottom, and is connected with incubator (1);Lateral partition (3- is equipped in the shell (3-1) 6), shell (3-1) internal cavities are divided into upper plenum (3-11) and lower cavity (3-12) by partition, set on the partition (3-6) There is the through-hole (3-61) for being connected to upper plenum and lower cavity, the connecting rod (3-3) is threaded onto partition (3-6), the sealing Circle (3-2) is fixed at the top of connecting rod (3-3), and sealing ring (3-2) overlapping edges are at the top of shell (3-1);Sealing ring (the 3- 2) it is less than or equal to the inner surface of incubator (1) bottom, first spring (3-4) is set on connecting rod (3-3), and under being located at In cavity (3-12);The lever (3-5) is fixed in the end connecting rod (3-3), and the height under lever (3-5) free state is high In the bottom surface of support.
3. the culture sampler according to claim 1 for marine bacteria, it is characterised in that: the control device It (4) include cylinder body (4-1), temperature-sensitive module (4-2), cylinder (4-3) and reset spring (4-4), the cylinder body (4-1) is fixed in training It supports on the inner sidewall of case (1) and is located at the gas vent (1-1) of incubator, first chamber (4- is equipped in the cylinder body (4-1) 11), second chamber (4-12), the first release channel (4-13) and the second release channel (4-14), the first release channel (4- 13) it is connected with first chamber (4-11) with the second release channel (4-14), the first chamber (4-11) of the cylinder body (4-1) One end be provided with temperature-sensitive module (4-2), the other end is connected with second chamber (4-12);The second chamber (4-12) and training The gas vent (1-1) supported on box body is connected, and the temperature-sensitive module (4-2) includes temperature susceptibility contact terminal (4-21), temperature-sensitive shell (4- 22) and diaphragm (4-23), the temperature-sensitive shell (4-22) are fixed on the port of cylinder body (4-1) first chamber (4-11), temperature-sensitive shell (4- 22) it is set diaphragm (4-23) in, outside is connected with temperature susceptibility contact terminal (4-21);The diaphragm (4-23) is connected with the one end cylinder (4-3), The cylinder (4-3) is threaded onto the first chamber (4-11) and second chamber (4-12) of cylinder body (4-1), and cylinder (4-3) The other end forms bulge (4-31), and the bulge (4-31) is located in second chamber (4-12), and second chamber (4-12) Port diameter be greater than the port diameter of first chamber (4-11), it is interior that the reset spring (4-4) is located at second chamber (4-12), And its both ends is inconsistent with the inner sidewall of the bulge (4-31) of cylinder (4-3) and cylinder body (4-1) respectively.
4. the culture sampler according to claim 3 for marine bacteria, it is characterised in that: further include baffle (4- 5), it is connected by rod piece (4-51) with the bulge (4-31) of cylinder (4-3), and rod piece (4-51) is threaded onto reset spring In (4-4);The baffle (4-5) is located at the gas vent (1-1) of incubator (1), and has a diameter larger than the straight of gas vent (1-1) Diameter.
5. the culture sampler according to claim 1 for marine bacteria, it is characterised in that: further include for filtering The Rose Box (5) of impurity, the Rose Box (5) are connected with incubator (1), and the Rose Box (5) includes end cap (5-1), holds Case (5-2) and support tube (5-3) are set, the end cap (5-1) is detachably connected with storage box (5-2), and end cap (5-1) is equipped with Inlet and outlet;The inlet and outlet is connected with storage box (5-2) respectively, and import is connected with extraneous initial water sample, Outlet is connected with incubator;The venthole (5-31) for filtering, the support are formed on support tube (5-3) side wall Cylinder (5-3) is located in storage box (5-2) and is connected by connector with end cap (5-1), the internal cavities of the support tube (5-3) It is connected with outlet.
6. the culture sampler according to claim 5 for marine bacteria, it is characterised in that: the support tube (5- 3) copper wire is wound on lateral wall.
7. the culture sampler according to claim 1 for marine bacteria, it is characterised in that: the locating piece (2- 6) center of circle in the rotation center of circle and turntable (2-3) is on same straight line.
8. the culture sampler according to claim 1 for marine bacteria, it is characterised in that: the agitator arm (2- 5) at least provided with upper and lower two arc shaped hooks (2-51) on.
CN201810433179.8A 2018-05-08 2018-05-08 Culture sampler for marine bacteria Active CN108865649B (en)

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CN112718032A (en) * 2020-12-28 2021-04-30 广州方笑生物科技有限公司 Reagent adds machine for biology

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN204022836U (en) * 2014-09-03 2014-12-17 周赞虎 Microbial cultivation device
CN206909366U (en) * 2017-07-25 2018-01-23 温州市洞头区水产科学技术研究所 A kind of marine climate for sargassum fusifome simulates culture apparatus
CN207210403U (en) * 2017-09-07 2018-04-10 北京双龙阿姆斯科技有限公司 A kind of culture apparatus

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN204022836U (en) * 2014-09-03 2014-12-17 周赞虎 Microbial cultivation device
CN206909366U (en) * 2017-07-25 2018-01-23 温州市洞头区水产科学技术研究所 A kind of marine climate for sargassum fusifome simulates culture apparatus
CN207210403U (en) * 2017-09-07 2018-04-10 北京双龙阿姆斯科技有限公司 A kind of culture apparatus

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