CN108855589A - A kind of magnetic separating device of biological sample - Google Patents
A kind of magnetic separating device of biological sample Download PDFInfo
- Publication number
- CN108855589A CN108855589A CN201810601592.0A CN201810601592A CN108855589A CN 108855589 A CN108855589 A CN 108855589A CN 201810601592 A CN201810601592 A CN 201810601592A CN 108855589 A CN108855589 A CN 108855589A
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- China
- Prior art keywords
- wall
- plate
- biological sample
- welded
- bolted
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Abstract
The invention belongs to biological treating equipment technical fields, especially a kind of magnetic separating device of biological sample, it is complicated for structure design comparison, higher operating costs, it and is open, easily cause the cross contamination between sample, step is many and diverse, and Magneto separate effect is bad, toxic reagent can be contacted, it is easy to pollute problem to experimenter and ambient enviroment, now propose following scheme, including cabinet, Magneto separate plate has been bolted in the bottom of box inner wall, the bottom of box outer wall is welded with supporting leg, and supporting leg number is two, link block has been bolted in the supporting leg bottom outer wall, and two link blocks are symmetrical.The present invention can the convenient and quick separation and purifying for efficiently completing biological sample, but also effectively avoid the cross contamination between sample, avoid some cumbersome procedures, alleviate the work load of people, and the present apparatus makes that pollution will not be generated in operation.
Description
Technical field
The present invention relates to biological treating equipment technical field more particularly to a kind of magnetic separating devices of biological sample.
Background technique
Currently, magnetic separation technique is a kind of technology that substance is carried out to magnetic field processing, the application of the technology has been penetrated into
Every field, the technology are the difference using element or component magnetic susceptibility, and substance is carried out magnetic field processing by external magnetic field,
Biochemical research and clinical application field, extract nucleic acid, protein etc. from the biological samples such as animal, plant, microorganism
Particular organisms component is the basis of one of routine techniques and follow-up study and experiment.Needed for being isolated in traditional slave sample
Component needs the process of the relatively complicated complexity such as grinding, mixed dissolution, centrifugation.For the existing maturation of these step existing markets
Product, but be only for single step, and separating effect is bad, will affect follow-up test.
Through retrieving, Chinese patent grant number is CN200880017869.X.Patent, disclose a kind of magnetic separating device,
Including body part, there is a surface and for providing the magnetized portion in magnetic field, the body part can stand on branch by the surface
On support face；And sample vessel retaining portion, it is used to keep at least one sample vessel.It is had the following disadvantages in above-mentioned patent：
Its structure design comparison is complicated, higher operating costs, and be it is open, easily cause the cross contamination between sample, step is numerous
It is miscellaneous, and Magneto separate effect is bad, can contact toxic reagent, is easy to pollute experimenter and ambient enviroment.And it is above-mentioned
Patent not can solve problems, therefore, need the magnetic separating device for designing a kind of biological sample.
Summary of the invention
Complicated, the higher operating costs based on structure design comparison, and be it is open, easily cause the intersection between sample dirty
Dye, step is many and diverse, and Magneto separate effect is bad, can contact toxic reagent, is easy to cause dirt to experimenter and ambient enviroment
Problem Technical problem is contaminated, the invention proposes a kind of magnetic separating devices of biological sample.
A kind of magnetic separating device of biological sample proposed by the present invention, including cabinet, the bottom of box inner wall pass through spiral shell
It tethers and is connected to Magneto separate plate, the bottom of box outer wall is welded with supporting leg, and supporting leg number is two, the supporting leg bottom
Link block has been bolted in portion's outer wall, and two link blocks are symmetrical, and the top of the box outer wall is bolted
There is sealing cover, cabinet side outer wall is welded with support plate, and the first electricity has been bolted in the support plate top exterior walls
Machine, one end outer wall of the first motor output shaft is welded with connecting rod, and the output shaft of first motor passes through outside cabinet side
Wall, the connecting rod are welded with extension sleeve far from one end outer wall of first motor, and extension sleeve side outer wall passes through spiral shell
It tethers and is connected to the second motor, the extension sleeve is socketed with rotating bar, the rotating bar surrounding far from one end outer wall of connecting rod
Outer wall is welded with the equidistant stirring rod being distributed in a ring, and the stirring rod is bolted far from one end outer wall of rotating bar
There is agitating paddle, and agitating paddle cross section is ellipse, cabinet two sides inner wall is welded with the first striker plate, the cabinet two
Side inner wall has been bolted fixed disk, and fixed disk inner wall is welded with the second striker plate, outside second striker plate two sides
Wall is provided with conveying runner, and the fixed disk top exterior walls are provided with material guide hole, and material guide hole is connected with conveying runner, described to lead
Material hole inner wall is plugged with passage, and flow sensor has been bolted inside the passage.
Preferably, fluid plate, and fluid plate covering and Magneto separate has been bolted in the Magneto separate plate top exterior walls
In the top exterior walls of plate, fluid plate side inner wall is provided with injection port, and fluid plate side inner wall is provided with outlet, outlet and sample introduction
Mouth is located at the two sides inner wall of fluid plate, and fluid plate bottom interior wall is provided with flow channel for liquids, and outlet and injection port pass through liquid
Runner is mutually connected, and flow channel for liquids is three branch roads, and fluid plate top exterior walls are provided with the hole equidistantly distributed, and hole is uniform
It is distributed in the side of flow channel for liquids, Magneto separate plate top exterior walls are provided with the magnetic patch of protrusion, the specification of magnetic patch and the specification of hole
Match, passage is plugged in hole.
Preferably, the link block bottom outer wall is provided with universal wheel, and universal wheel middle position is fixedly connected with service brake,
Service brake one end outer wall is welded with foot-operated.
Preferably, the sealing cover is located at the surface of feed pipe, and sealing cover surrounding outer wall is provided with seal groove, charging
The equal adhesive seal item of pipe surrounding outer wall, sealing strip clamping with seal groove inside.
Preferably, described rotating bar one end outer wall is welded with brush plate, and outer wall stickup in brush plate side has bristle firmly.
Preferably, the middle position of the first striker plate bottom outer wall is provided with the second opening, and the second open interior is inserted
It is connected to discharge nozzle, discharge nozzle is internally provided with control valve.
Preferably, processor has been bolted in cabinet side outer wall, shape between processor and flow sensor
At electric connection.
Preferably, the top of the box outer wall is provided with the first opening, and is welded with feed pipe in the first opening.
Preferably, the agitating paddle outer wall is provided with through-hole, and the diameter of through-hole is all identical.
Preferably, groove is provided with inside the supporting leg, and groove top and bottom interior wall are welded with damping spring.
Having the beneficial effect that in the present invention：
1, by the fluid plate of setting, can the convenient and quick separation and purifying for efficiently completing biological sample, be suitable for automatic
Change biology, medicine detection, fields such as molecular diagnosis, and very easy to use, but also effectively avoids between sample
Cross contamination has biggish application prospect.
2, by the flow channel for liquids of setting, because flow channel for liquids is that three branch roads are more conducive to being sufficiently mixed for reagent,
Make Magneto separate better effect, avoids the need for these mistakes that people need the relatively complicated complexity such as grinding, mixed dissolution, centrifugation
Journey alleviates the work load of people, reduces the labor intensity of people.
3, it by the agitating paddle of setting, can say that nano particle is quickly sufficiently mixed with biological sample reagent, save big
The time is measured, accelerates the Magneto separate of biological sample, and easy to operate.
4, by the Magneto separate plate of setting, the generation of toxic reagent such case can be contacted by avoiding traditional separation method,
And the present apparatus makes that pollution will not be generated in operation, avoids the infection of experimenter and the pollution to ambient enviroment, improves
The efficiency of sample process.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the magnetic separating device of biological sample proposed by the present invention；
Fig. 2 is a kind of fluid plate structural schematic diagram of the magnetic separating device of biological sample proposed by the present invention；
Fig. 3 is a kind of Magneto separate plate structural schematic diagram of the magnetic separating device of biological sample proposed by the present invention；
Fig. 4 is a kind of fixed disk structural schematic diagram of the magnetic separating device of biological sample proposed by the present invention；
Fig. 5 is a kind of agitating paddle structural schematic diagram of the magnetic separating device of biological sample proposed by the present invention.
In figure：1 cabinet, 2 Magneto separate plates, 3 supporting legs, 4 damping springs, 5 universal wheels, 6 fluid plates, 7 injection ports, 8 service brakes,
It is 9 link blocks, 10 grooves, 11 processors, 12 support plates, 13 first motors, 14 connecting rods, 15 second motors, 16 feed pipes, 17 close
Capping, 18 extension sleeves, 19 rotating bars, 20 brush plates, 21 discharge nozzles, 22 first striker plates, 23 fixed disks, 24 flow sensings
Device, 25 passages, 26 holes, 27 flow channel for liquids, 28 outlets, 29 magnetic patch, 30 second striker plates, 31 conveying runners, 32 guides
Hole, 33 stirring rods, 34 through-holes, 35 agitating paddles.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Referring to Fig.1-5, a kind of magnetic separating device of biological sample, including cabinet 1,1 bottom interior wall of cabinet are connected by bolt
It is connected to Magneto separate plate 2,1 bottom outer wall of cabinet is welded with supporting leg 3, and 3 number of supporting leg is two, 3 bottom outer wall of supporting leg
Link block 9 is bolted, and two link blocks 9 are symmetrically, sealing cover has been bolted in 1 top exterior walls of cabinet
17,1 side outer wall of cabinet is welded with support plate 12, and 12 top exterior walls of support plate have been bolted first motor 13, and first
One end outer wall of 13 output shaft of motor is welded with connecting rod 14, and the output shaft of first motor 13 passes through 1 side outer wall of cabinet, even
Extension bar 14 is welded with extension sleeve 18 far from one end outer wall of first motor 13, and 18 side outer wall of extension sleeve is bolted
There is the second motor 15, extension sleeve 18 is socketed with rotating bar 19,19 surrounding outer wall of rotating bar far from one end outer wall of connecting rod 14
It is welded with the equidistant stirring rod 33 being distributed in a ring, stirring rod 33 has been bolted far from one end outer wall of rotating bar 19
Agitating paddle 35, and 35 cross section of agitating paddle is ellipse, 1 two sides inner wall of cabinet is welded with the first striker plate 22,1 two sides of cabinet
Fixed disk 23 has been bolted in inner wall, and 23 inner wall of fixed disk is welded with the second striker plate 30,30 two sides of the second striker plate
Outer wall is provided with conveying runner 31, and 23 top exterior walls of fixed disk are provided with material guide hole 32, and material guide hole 32 connects with conveying runner 31
Logical, 32 inner wall of material guide hole is plugged with passage 25, and flow sensor 24 has been bolted inside passage 25.
In the present invention, fluid plate 6, and the covering of fluid plate 6 and Magneto separate has been bolted in 2 top exterior walls of Magneto separate plate
In the top exterior walls of plate 2,6 side inner wall of fluid plate is provided with injection port 7, and 6 side inner wall of fluid plate is provided with outlet 28, outlet
28 are located at the two sides inner wall of fluid plate 6 with injection port 7, and 6 bottom interior wall of fluid plate is provided with flow channel for liquids 27, outlet 7 with
Injection port 7 is mutually connected by flow channel for liquids 27, and flow channel for liquids 27 is three branch roads, and 6 top exterior walls of fluid plate are provided with equidistant
The hole 26 of distribution, hole 26 are uniformly distributed in the side of flow channel for liquids 27, and 2 top exterior walls of Magneto separate plate are provided with protrusion
Magnetic patch 29, the specification of magnetic patch 29 and the specification of hole 26 match, and passage 25 is plugged in hole 26.
9 bottom outer wall of link block is provided with universal wheel 5, and 5 middle position of universal wheel is fixedly connected with service brake 8, service brake 8 one
Outer wall is held to be welded with foot-operated, sealing cover 17 is located at the surface of feed pipe 16, and 17 surrounding outer wall of sealing cover is provided with seal groove,
The equal adhesive seal item of 16 surrounding outer wall of feed pipe, with inside seal groove, 19 one end outer wall of rotating bar welds hairiness for sealing strip clamping
Brush board 20, and the stickup of 20 side outer wall of brush plate has bristle firmly, the middle position of 22 bottom outer wall of the first striker plate is provided with second
Opening, and the second open interior is plugged with discharge nozzle 21, discharge nozzle 21 is internally provided with control valve.
Processor 11, and 11 model ARM9TDMI of processor, processor 11 has been bolted in 1 side outer wall of cabinet
Be electrically connected between flow sensor 24,1 top exterior walls of cabinet are provided with the first opening, and be welded in the first opening into
Expects pipe 16,35 outer wall of agitating paddle is provided with through-hole 34, and the diameter of through-hole 34 is all identical, groove 10 is provided with inside supporting leg 3, and recessed
10 top and bottom inner wall of slot is welded with damping spring 4.
In use, staff opens sealing cover 17, sample reagent and nano particle are directed into cabinet 1 from feed pipe 16
Inside starts first motor 13, and first motor 13 rotates, and connecting rod 14 is driven to be rotated, and starts the second motor 15, the second electricity
Machine 15 makes rotating bar 19 stretch in extension sleeve 18, since extension sleeve 18 is connected with rotating bar 19, rotating bar
19 rotations drive extension sleeve 18 to rotate, and therefore, rotating bar 19 drives agitating paddle 35 that can carry out Telescopic rotating to sample reagent
It is adequately mixed with nano particle, the brush plate 20 of 19 one end of rotating bar can be mixed to 1 inner wall of cabinet is pasted onto
It closes liquid to be stirred again, the mixing after being sufficiently stirred flows into discharge nozzle 21 by the first striker plate 22, opens control valve, mixes
It closes liquid to flow into fixed disk 23 by discharge nozzle 21, the mixed liquor in fixed disk 23 is by conveying runner 31 out of deflector hole 32
Outflow, the mixed liquor flowed out out of material guide hole 32 are flowed into passage 25, are entered in fluid plate 6 by passage 25, are mixed
Liquid is flowed into Magneto separate plate 2 by hole 26 by flow channel for liquids 27, will after Magneto separate is carried out in Magneto separate plate 2
Biological sample is eluted from magnetic nanoparticle, and eluent is collected to the sample for obtaining separator well after the outflow of outlet 28
This.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of magnetic separating device of biological sample, including cabinet（1）, which is characterized in that the cabinet（1）Bottom interior wall passes through
It has been bolted Magneto separate plate（2）, the cabinet（1）Bottom outer wall is welded with supporting leg（3）, and supporting leg（3）Number is two
It is a, the supporting leg（3）Link block has been bolted in bottom outer wall（9）, and two link blocks（9）Symmetrically, described
Cabinet（1）Sealing cover has been bolted in top exterior walls（17）, the cabinet（1）Side outer wall is welded with support plate（12）,
The support plate（12）First motor has been bolted in top exterior walls（13）, the first motor（13）One end of output shaft
Outer wall is welded with connecting rod（14）, and first motor（13）Output shaft pass through cabinet（1）Side outer wall, the connecting rod（14）
Far from first motor（13）One end outer wall be welded with extension sleeve（18）, the extension sleeve（18）Side outer wall passes through bolt
It is connected with the second motor（15）, the extension sleeve（18）Far from connecting rod（14）One end outer wall be socketed with rotating bar（19）,
The rotating bar（19）Surrounding outer wall is welded with the equidistant stirring rod being distributed in a ring（33）, the stirring rod（33）It is separate to turn
Lever（19）One end outer wall agitating paddle has been bolted（35）, and agitating paddle（35）Cross section is ellipse, the case
Body（1）Two sides inner wall is welded with the first striker plate（22）, the cabinet（1）Fixed disk has been bolted in two sides inner wall
（23）, and fixed disk（23）Inner wall is welded with the second striker plate（30）, second striker plate（30）Two sides outer wall is provided with defeated
Stream road（31）, the fixed disk（23）Top exterior walls are provided with material guide hole（32）, and material guide hole（32）With conveying runner（31）Phase
It connects, the material guide hole（32）Inner wall is plugged with passage（25）, the passage（25）Flow has been bolted in inside
Sensor（24）.
2. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the Magneto separate plate（2）
Fluid plate has been bolted in top exterior walls（6）, and fluid plate（6）Covering and Magneto separate plate（2）Top exterior walls on, fluid
Plate（6）Side inner wall is provided with injection port（7）, fluid plate（6）Side inner wall is provided with outlet（28）, outlet（28）With injection port
（7）It is located at fluid plate（6）Two sides inner wall, fluid plate（6）Bottom interior wall is provided with flow channel for liquids（27）, outlet（7）With
Injection port（7）Pass through flow channel for liquids（27）It mutually connects, flow channel for liquids（27）For three branch roads, fluid plate（6）Top exterior walls are opened
There is the hole equidistantly distributed（26）, hole（26）Uniformly it is distributed in flow channel for liquids（27）Side, Magneto separate plate（2）Top
Outer wall is provided with the magnetic patch of protrusion（29）, magnetic patch（29）Specification and hole（26）Specification match, passage（25）Grafting
In hole（26）It is interior.
3. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the link block（9）Bottom
Portion's outer wall is provided with universal wheel（5）, and universal wheel（5）Middle position is fixedly connected with service brake（8）, service brake（8）The weldering of one end outer wall
It is connected to foot-operated.
4. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the sealing cover（17）Position
In feed pipe（16）Surface, and sealing cover（17）Surrounding outer wall is provided with seal groove, feed pipe（16）Surrounding outer wall is viscous
Paste sealing strip, sealing strip clamping with seal groove inside.
5. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the rotating bar（19）One
End outer wall is welded with brush plate（20）, and brush plate（20）Side outer wall is pasted bristle firmly.
6. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that first striker plate
（22）The middle position of bottom outer wall is provided with the second opening, and the second open interior is plugged with discharge nozzle（21）, discharge nozzle（21）
It is internally provided with control valve.
7. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the cabinet（1）Side
Processor has been bolted in outer wall（11）, processor（11）With flow sensor（24）Between be electrically connected.
8. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the cabinet（1）Top
Outer wall is provided with the first opening, and is welded with feed pipe in the first opening（16）.
9. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the agitating paddle（35）Outside
Wall is provided with through-hole（34）, and through-hole（34）Diameter it is all identical.
10. a kind of magnetic separating device of biological sample according to claim 1, which is characterized in that the supporting leg（3）It is interior
Portion is provided with groove（10）, and groove（10）Top and bottom inner wall is welded with damping spring（4）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810601592.0A CN108855589B (en) | 2018-06-12 | 2018-06-12 | Magnetic separation device for biological samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810601592.0A CN108855589B (en) | 2018-06-12 | 2018-06-12 | Magnetic separation device for biological samples |
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| Publication Number | Publication Date |
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| CN108855589A true CN108855589A (en) | 2018-11-23 |
| CN108855589B CN108855589B (en) | 2020-01-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810601592.0A Active CN108855589B (en) | 2018-06-12 | 2018-06-12 | Magnetic separation device for biological samples |
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| CN207238254U (en) * | 2017-11-26 | 2018-04-17 | 鸿泰（清远）铝业有限公司 | A kind of magnetic material sorting equipment with Separation by vibration effect |
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| UA14724U (en) * | 2005-12-20 | 2006-05-15 | Mykola Frantsovych Rozhkivskyi | Rozhkivskyiæs concentrated-feed aggregate |
| CN205761753U (en) * | 2016-05-28 | 2016-12-07 | 贵州中伟投资集团有限公司 | A kind of glass production material deironing device |
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Effective date of registration: 20191225 Address after: 225316 building 27, No. 801, Jiankang Avenue, Taizhou City, Jiangsu Province (Yaocheng) Applicant after: Jiangsu Yao Hai biopharmaceutical Co., Ltd. Address before: 450046 Room 516, Building 2, Zheng Shang Muhua Square, Mingli Road, Zhengdong New District, Zhengzhou City, Henan Province Applicant before: Zhengzhou Zhi Zhi Zhi Electronic Technology Co., Ltd. Applicant before: Zhengzhou Institute of Aeronautical Industry Management |
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